Degree Of Relevance Research Articles

Carnivore salarymen and herbivore males: Kimi no Na wa and the shift to the omnivorous person

ABSTRACTConcerns of gender equality and fluidity has been a long‐standing issue in contemporary society for decades, however, recently, the pressing issue surrounding media representations of gender has come to the forefront. The rise of feminist studies over the last few decades has led to a cornucopia of research on manga and anime in relation to femininity, but fewer examine masculinity or gender fluidity. This paper will expand the study of both femininity and masculinity but will mainly focus on masculinity, in the form of the Carnivore Woman and the Herbivore Man, with an argument towards the “Omnivorous Person.” The omnivorous person can be thought of as an extension and paralleling to gender fluidity. As a highly accessible medium, through fantasy and metaphor, anime and manga have the ability to display a collective consciousness of Japanese society. To further elaborate on these new gender trends within Japanese popular media, a textual analysis of the anime film Kimi no Na wa (2016) will be used as exemplar. It is impossible to conduct any in‐depth study that is truly representative of a genre, especially considering the magnitude and vastness of manga and anime, however, selecting a text that is “popular” can suppose some degree of cultural relevance.

Navigating the Digital Landscape: The Role of Influencer Marketing in Boosting Sales

This research paper specifically tries to analyse the interconnectivity of digital marketing and influencer marketing, especially about sales. The author’s purpose is to identify the effective promotion tactics and engagement of the consumer using influencer marketing. The following secondary research method was used; a comprehensive search of already published sources was conducted and several case studies were used to gather data analysing the effectiveness of influencer marketing in changing consumer buying behaviour and sales results. Some studies show that the level of the message and the degree of campaign relevance to brand values affect the effectiveness of the campaign. Finally, the research finds a new driving force in influencer marketing, but at the same time, brands face many issues with evaluation and credibility to influence sales.

3-Diazopiperidine-2,4-diones as Convenient Precursors to Rare Polysubstituted Spiro Bis-β,γ-lactams and 2-Oxopyrrolidine-3-carboxylic Acid Derivatives via the Thermally Promoted Wolff Rearrangement.

Herein, we describe a diastereoselective and straightforward synthetic approach to polysubstituted spirocyclic bis-lactams bearing both β- and γ-lactam cores with diverse substitution patterns. The method developed is based on a microwave-assisted Wolff rearrangement/Staudinger [2 + 2] cycloaddition sequence involving 3-diazopiperidine-2,4-diones and imines. The corresponding reaction tolerates a wide range of functionalities in both substrates, giving the target bis-lactams in generally high yields. The synthetic utility of the thermally promoted Wolff rearrangement has been extended to the interaction of 3-diazopiperidine-2,4-dione-derived ketenes with various nucleophiles leading to derivatives of 2-oxopyrrolidine-3-carboxylic acids. Our findings potentially pave an avenue for designing new compounds with a high degree of medicinal relevance by means of the processes studied.

Chromium supplementation and type 2 diabetes mellitus: an extensive systematic review.

Diabetes is a global public health concern with increasing prevalence worldwide. Chromium (Cr), a trace element found in soil, water, and food, has been proposed to have a possible positive effect in glucose metabolism and diabetes mellitus prevention. However, the relationship between trivalent chromium [Cr(III)] exposure, mainly through the consumption of diet supplements, and type 2 diabetes mellitus (T2DM) remains controversial. An extensive systematic review of the current literature on randomized controlled studies (RCTs) was conducted from 1 January 2000, to January 2024 using the databases PubMed, Scopus, ScienceDirect, and Cochrane, with specific keywords and inclusion as well as exclusion criteria. After close screening of the research studies retrieved from the mentioned websites was conducted, the most related studies were included in the final systematic review. The studies were evaluated for the degree of relevance, quality, and risk bias, using appropriate quality assessment tools. Several of the included RCT studies reported possible benefits of Cr(III) supplementation, mainly in the form of chromium picolinate (CrPic), chromium yeast (CY), chromium chloride (CrCl3), and chromium nicotinate (CrN). The dosage of chromium was between 50 and 1000μg/day and it was consumed from 2 to 6months. Glycemic control markers, including FPG, insulin, HbA1C, and HOMA-IR levels, significantly decrease following chromium supplementation, mainly in studies with a longer intervention period. Supplementing with chromium (Cr) indicated that could significantly improve lipid profile by raising high-density lipoprotein and lowering triglyceride and total cholesterol while having little effect on low-density lipoprotein. However, most research findings include significant limitations, such as inconsistent dosage and type of chromium, formulation of supplements, and study duration. Further well-designed and high-quality research is needed to fully understand the role of chromium dietary supplementation and the potential risks related to its mechanisms of action, type, and dose, in the prevention and treatment of type 2 diabetes mellitus.

University-based academic programs in addiction studies in the regions of Australia and Aotearoa New Zealand: An overview.

Current trends in the addiction field reflect a significant emphasis on the workforce development and education. There are already some data about university-based addiction studies programs, but not much from Australasia. The aim is to provide an overview and describe the academic programs for addiction professionals in Australia and Aotearoa NZ. The research was conducted in 2017 and updated in 2023. Firstly, university websites were searched using pre-defined keywords, followed by a content analysis of the identified programs. The data were analysed and interpreted by using descriptive statistics. We found 21 universities in Australia (13) and Aotearoa NZ (8) where 46 single programs are provided. There are three bachelor programs, nine masters, and the majority of degrees include (post)graduate certificates and diplomas. No doctorate programs are identified. The taught courses provide comprehensive coverage of the addiction field topics. Twelve programs state clearly that there is clinical practice/internship included. Application to most programs requires completion of a relevant degree and in some cases possible clinical experience. In comparison to educational options in other regions, we observe a trend towards preparing university graduates for the workforce, thereby expanding the range of programs at lower levels. Most programs possibly represent clinically oriented education primarily specialising in addictions, and graduate programs in addictions for professionals with other disciplinary bases. Great emphasis is given to the quality standards of education, and also to relationship between education and labour market. Findings help opening opportunities to collaborate globally.

Perceptions of undertaking a higher degree alongside dental specialty training: A cross-sectional survey of UK dental specialty trainees.

The curricula for UK dental specialty training have recently been under review and until 2024, completion of a research component during training in Dental Public Health, Oral Microbiology and Orthodontics has been mandatory (with an alternative route for Orthodontics involving the submission of two scientific papers for those trainees not wishing to undertake a higher degree). Anecdotally, some trainees in other dental specialties choose to undertake higher degrees alongside specialty training. The aims were to investigate how many dental specialty registrars study for higher degrees alongside specialty training, and whether undertaking a higher degree alongside specialty training has an impact on completion of training, research skills, research experience, patient care and career opportunities. This was a cross-sectional study design, involving the distribution of an online, anonymous questionnaire-based survey to UK dental specialty registrars in November and December 2022. In total, 38 questionnaires were completed, representing a 7.7% response rate of the entire dental specialty registrar cohort in the UK and 42% of those who received it. Most respondents (76.3%) were either studying or had completed a clinically relevant higher degree prior to specialty training. Most respondents (76.3%) reported that the higher degree increased career opportunities and gave them additional skills. Dental specialty trainees who responded to this survey perceived the higher degree to be beneficial in terms of preparing for exams, gaining skills in critical appraisal and for increasing future career opportunities.

Impact of CCR5Δ32 on the risk of infection, Staphylococcus aureus carriage, and plasma concentrations of chemokines in Danish blood donors

The CC chemokine receptor 5 (CCR5) is a suggested receptor for Staphylococcus aureus leukotoxin ED. Homozygosity for the Δ32 deletion (CCR5Δ32) protects against human immunodeficiency virus infection and possibly also against leukotoxin ED. We examined the impact of CCR5Δ32 on the susceptibility to S.aureus infection, all-cause infections, and S.aureus nasal carriage, respectively, and on the concentrations of circulating chemokines in blood donors. We included 95,406 participants from the Danish Blood Donor Study (DBDS) genotyped for >650,000 single nucleotide polymorphisms. The CCR5Δ32 (rs333, MAF: 0.12) was imputed from a reference panel and validated. Infectious outcomes were identified by diagnosis codes and redeemed prescription of antibiotics in national health registers. Data on S.aureus nasal carriage and forty-seven inflammatory biomarkers were available for 6721 and 7811 participants, respectively. Cox, logistic, and linear regression models adjusted for relevant confounders were used to explore said associations. During more than 700,000 person-years of observation, we found that CCR5Δ32 was associated with neither an increased risk of redeemed dicloxacillin, hospital-treated S.aureus-associated infection (replicated in 345,996 Icelanders), redeemed antibiotics, all-cause infection, and nor with S.aureus nasal carriage. We discovered an association between CCR5Δ32 and elevated CCL4 concentrations, which were 1.26-fold higher in Δ32-heterozygotes (95%-CI: 1.23-1.30) and 2.64-fold higher in Δ32-homozygotes (95%-CI: 2.41-2.90) compared with wildtype homozygotes. Conversely, concentrations of CCL2, CXCL-10, and CCL11 were slightly lower among Δ32-heterozygotes. Results from this CCR5Δ32 high-prevalent cohort do not support the idea that CCR5Δ32 affects the risk of S.aureus carriage or infection to any relevant degree, in this northern European context. CCL4 was the main chemokine affected by CCR5Δ32 and was observed in higher concentration among Δ32-carriers. This study cannot rule out that S.aureus is a previous driver of CCR5Δ32 selection. The Health Research Fund of Central Denmark Region, Aarhus University, Danish Administrative Regions, Bio- and Genome Bank Denmark, Danish Blood Donor Research Foundation, Aase & Ejnar Danielsens Foundation, Højmosegård Grant, National Institute of Allergy and Infectious Diseases, and A.P. Møller Foundation for the Advancement of Medical Science.

Research on the Linkage between the Professional Degree of Master of Intellectual Property and Professional Qualifications

With the vigorous development of the intellectual property (IP) industry, the national IP talent pool has exceeded 700,000 individuals, posing higher demands on the cultivation of specialized talents. In 2022, the Academic Degrees Committee of the State Council and the Ministry of Education introduced a new professional degree category for IP, but in its initial stages, it confronted challenges such as imbalanced curriculum systems, inadequate teaching resources, and restricted enrollment in professional qualifications. Simultaneously, the connections between the IP professional degree and national unified legal professional qualifications, patent attorney qualifications, IP professionals, and other professional certifications point to a high degree of consistency and mutual relevance in talent cultivation objectives. The professional degree education must reference the content of professional qualification certifications to ensure that talents meet industry demands. Additionally, the professional degree education exerts a positive influence on the professional entry qualification certification, elevating the overall standards within the professional field.

Testing Whether Gravity Acts as a Quantum Entity When Measured.

A defining signature of classical systems is "in principle measurability" without disturbance: a feature manifestly violated by quantum systems. We describe a multi-interferometer experimental setup that can, in principle, reveal the nonclassicality of a spatial superposition-sourced gravitational field if an irreducible disturbance is caused by a measurement of gravity. While one interferometer sources the field, the others are used to measure the gravitational field created by the superposition. This requires neither any specific form of nonclassical gravity, nor the generation of entanglement between any relevant degrees of freedom at any stage, thus distinguishing it from the experiments proposed so far. This test, when added to the recent entanglement-witness based proposals, enlarges the domain of quantum postulates being tested for gravity. Moreover, the proposed test yields a signature of quantum measurement induced disturbance for any finite rate of decoherence, and is device independent.

Digital Storytelling as a Method of Achieving Visual Literacy

The research aims to explore the pedagogical benefits of the digital storytelling tool on the acquisition of visual literacy among higher education learners, as well as their personal attitudes during the process of completion of the specific task. Two main questions were asked: “Does digital storytelling support the development of key skills related to visual literacy?” and “Do the learners feel sufficiently engaged and motivated in the specific pedagogical tool of digital storytelling?” A qualitative approach was used using the method of direct observation and a survey with free-text answers. As in our case, Digital story is intended for students studying audiovisual disciplines in higher education, we have significantly raised the performance and assessment criteria so as to be adequate to the level of professional training to be covered by the relevant academic degree. The subjective evaluations of the students clearly showed a positive attitude towards the learning process, and the objective teaching observations brought out the presence of achievements in an upward direction. The important teaching conclusions are that, in a pedagogical aspect, Digital story fulfilled its purpose – to assist in a light and fun way to master audiovisual technical and creative skills at a high professional level, to engage learners with course content, to have a lasting impact on higher order visual literacy skills, to effectively develop visual thinking.

GENERALIZED PASCAL’S PYRAMIDS AND COMBINATORIAL INFORMATION RETRIEVAL PROBLEMS

The work studies combinatorial objects of pyramidal structure. It proposes fundamental relations for generalizations of Pascal’s triangle and pyramid. It considers problems of finding new ways of constructing information relevance indices and offers a way of constructing such an index with the help of Pascal’s generalized pyramids. Using structures of a type of Pascal’s generalized pyramids with weight factors allow construction of an index that reflects a degree of relevance of keywords, as well as makes it possible to allocate subsets of keywords with specific properties. The offered methods of the combinatorial analysis of hierarchical structures in information retrieval problems can be used in creating and analyzing knowledge databases.

Design of an Educational Psychology Research Material Sharing Platform Based on Fuzzy Clustering Algorithm

In the interdisciplinary field of educational psychology, efficiently managing and accessing research materials remains a formidable challenge due to the vast and varied nature of available data. This paper introduces an innovative platform engineered to streamline the organization and dissemination of educational psychology research materials by harnessing the power of fuzzy clustering algorithms for automated content categorization. Motivated by the imperative to enhance the accessibility and discoverability of scholarly resources, our methodology employs the Fuzzy C-Means (FCM) algorithm, adept at accommodating the intrinsic ambiguity and overlap prevalent in educational psychology topics. The process initiates with a comprehensive preprocessing of diverse research materials, including scholarly articles, reports, and datasets, succeeded by meticulous feature extraction to distill pivotal themes and concepts. Subsequent application of the FCM algorithm facilitates the intuitive grouping of materials into clusters, each with varying degrees of relevance, thereby mirroring the multidimensional essence of the domain. Rigorous experimental validation of our approach underscores its efficacy in augmenting search precision and markedly diminishing the time scholars expend in locating pertinent materials. The platform’s user-centric interface further simplifies the exploration of educational psychology research, offering scholars a streamlined mechanism to access and engage with an extensive repository of resources. Through this study, we demonstrate a significant stride towards overcoming the hurdles in research material categorization and retrieval, heralding a new era of efficiency and accessibility for the educational psychology academic community on a global scale.

Learner training for phonetic transcription of typical and/or disordered speech: A scoping review.

Phonetic transcription is a core skill of speech and language therapists/pathologists (SLT/Ps) for clinical assessment of speech sounds and/or errors, and linguists for investigation of phonetic phenomena in various languages; hence, it is included in the curriculum of the corresponding degree programme. Many experts and course instructors have discussed their opinions on different aspects of phonetic transcription teaching and/or reported their own training programmes. However, no review has systematically summarized the types of expert recommendations and training methodology reported in the literature. Such information is important for mapping the knowledge gaps, refining current teaching and planning further research. To systematically summarize (1) the materials and procedures for delivering and evaluating phonetic transcription training programmes, and (2) the opinions from experts in phonetic transcription teaching regarding but not limited to the content, rationale(s), format and structure, and timing of teaching phonetic transcription of typical speech and/or disordered speech reported in the literature. A scoping review was carried out by following the Joanna Briggs Institute methodology. PubMed, EBSCOhost and Web of Science were searched and citation searching of included papers was completed. The included papers were divided into training programme reports, of which data (e.g., type and number of speech stimuli used, type and number of learners, outcome measures) were charted, and expert opinion papers, analysed using content analysis. A total of 565 studies were retrieved. After excluding duplicates and irrelevant papers and merging two sources that reported the same training, a total of 23 sources on training programmes and six opinion papers were analysed. Most training were for English and for SLT/P students, with some for the linguistics students. There are variations in the training content (with phonetic transcription practice as the main procedure), delivery mode (some presented via websites or computer software), stimuli (audio recordings of typical adults and children with speech disorders were most used), feedback (mostly immediate feedback using answer keys) and outcome measures (mainly transcription accuracy of learners and user opinions). Content analysis of opinion papers determined five main categories: rationale for training; aspects of the delivery of training; transcription; problem areas noted; and strategies/resources. Implications and considerations for teaching are discussed, along with recommendations for research in the design of evidence-based training. The findings can contribute to the development of general guidelines about phonetic transcription training in educational programmes and the establishment of pre-registration competencies. What is already known on this subject Phonetic transcription is a core skill of SLT/Ps and linguists for clinical speech assessment and research, and it is an important component in the curriculum of the relevant degree programmes. Many experts and course instructors have discussed their opinions regarding phonetic transcription teaching and/or reported their own training programmes in various sources such as books and book chapters, research papers and conference proceedings. What this paper adds to the existing knowledge The paper reports a scoping review that systematically summarized the materials and procedures for delivering and evaluating phonetic transcription programmes and experts' opinions and recommendations for phonetic transcription teaching, reported in the literature. The data from these two types of sources allowed an integrated discussion of the various aspects of phonetic transcription training, such as content, format and structure, materials and timing of teaching and the evaluation of training efficacy. What are the potential or actual clinical implications of this work? The data synthesized and discussed provide a foundation for a review of current practice in phonetic transcription teaching in SLT education. The findings can contribute to the development of general guidelines about phonetic transcription training in educational programmes and the establishment of pre-registration competencies.

A Deep Learning-Driven Sampling Technique to Explore the Phase Space of an RNA Stem-Loop.

The folding and unfolding of RNA stem-loops are critical biological processes; however, their computational studies are often hampered by the ruggedness of their folding landscape, necessitating long simulation times at the atomistic scale. Here, we adapted DeepDriveMD (DDMD), an advanced deep learning-driven sampling technique originally developed for protein folding, to address the challenges of RNA stem-loop folding. Although tempering- and order parameter-based techniques are commonly used for similar rare-event problems, the computational costs or the need for a priori knowledge about the system often present a challenge in their effective use. DDMD overcomes these challenges by adaptively learning from an ensemble of running MD simulations using generic contact maps as the raw input. DeepDriveMD enables on-the-fly learning of a low-dimensional latent representation and guides the simulation toward the undersampled regions while optimizing the resources to explore the relevant parts of the phase space. We showed that DDMD estimates the free energy landscape of the RNA stem-loop reasonably well at room temperature. Our simulation framework runs at a constant temperature without external biasing potential, hence preserving the information on transition rates, with a computational cost much lower than that of the simulations performed with external biasing potentials. We also introduced a reweighting strategy for obtaining unbiased free energy surfaces and presented a qualitative analysis of the latent space. This analysis showed that the latent space captures the relevant slow degrees of freedom for the RNA folding problem of interest. Finally, throughout the manuscript, we outlined how different parameters are selected and optimized to adapt DDMD for this system. We believe this compendium of decision-making processes will help new users adapt this technique for the rare-event sampling problems of their interest.

Some Developments and Vulnerabilities in the Child Rights Protection System under the Umbrella of the European Family

Bearing in mind that the European Economic Community was originally a purely economic construction, there is no consistent and relevant degree of conventionality in the relationship between the EU, on the one hand, and the family, in all its configuration, on the other. However, indirectly, through its own policies, the Union has shaped certain aspects of the concept of family, the free movement of European citizens and their family members being a case in point. In this study we aim to determine to what extent we can speak of the existence of the concept of the European family and to present the European standards that circumscribe the child in a family unit, including the adaptation of some "classic" rights of EU law to best suit the specific interests and needs of the child. Last but not least, we aim to present some vulnerabilities in the European system of protection of children's rights, caused either by inconsistency of the European legislator or by a lack of refinement in the interpretation of legal provisions relating to fundamental rights. Keywords: Child; Freedom of movement; Citizenship; Family member

Precession, nutation, and dynamic trajectory of magnetic rod in axisymmetric magnetic field

Precession and nutation in dynamical systems have been of long-standing research interest. Manifesting in systems as diverse as spinning tops to celestial bodies like planets, understanding these motions offers a window into the intricate dance of forces governing such systems. One example of a previously unexplored system exhibiting precession and nutation is that of a magnetic rod in an axisymmetric magnetic field. This project aims to study this system through qualitative, quantitative, and experimental means. A theoretical model was formulated using Newton's Second Law and the torque equation, accounting for energy dissipation. One unique aspect of this system compared to other systems exhibiting precession and nutation is the presence of the non-linear magnetic force and torque, which were modelled by considering both magnets as current-carrying cylinders. Experimentally, the dynamic trajectory and relevant degrees of freedom of the rod were measured using image binarization and ellipse fitting. This was used for a systematic investigation into how the nutation, precession and petaloid-like trajectories are affected by the number of magnets, initial angular velocity and other relevant parameters. Theoretical predictions and experimental results show a good agreement. This work has various engineering applications such as centrifuge design.

Going Underground: Demographics, Services, and Best Practices Endorsed by Practitioners Providing Support for Naturalistic Psychedelic Use

ABSTRACT Psychedelic-assisted therapy (PAT) has shown preliminary efficacy for psychiatric and physical health conditions. Although some people report naturalistic psychedelic use with so-called “underground” practitioners, little is known about PAT that occurs outside of controlled clinical settings or perspectives of these practitioners. We conducted an anonymous online survey of individuals who reported providing psychedelic support services (e.g. trip sitting and/or preparatory/follow-up psychotherapy) in naturalistic settings. We investigated demographics, including education and licensing, details about services provided, and reported client outcomes. Among 107 participants, 40.2% held a full or in-progress license and 44.9% had not obtained a relevant graduate degree. Almost all participants reported pre-screening clients before treatment, offering preparation, integration, and trip-sitting services, and most employed a range of therapeutic modalities, centering primarily on non-directive approaches. Participants reported that clients most commonly consumed psilocybin, and treated numerous conditions, primarily aligning with indications targeted in psychedelic clinical research. Perceptions of clients’ symptom changes were largely positive, although a small proportion reported worsened personality disorder symptoms. Further research delineating client and practitioner perspectives of naturalistic PAT services is warranted, and such work may shed light on the benefits and risks specific to naturalistic PAT as well as inform best practices for practitioners.

Problem of Low-Frequency Motion Cueing Along Roll on Flight Simulators

An innovative approach to improve the fidelity of low-frequency lateral motion cueing within full-flight simulators for non-maneuvering aircraft is presented. On the basis of the perception peculiarities by the human vestibular system of movement along the roll, the problem of low-frequency motion cueing of lateral movement by motion systems of flight simulators was formulated and solved. The critical challenge of simulating the set of angular motion cues that pilots perceive during flights is decided. This research highlights two key outcomes. The fourth-order low-pass filter effectively extracts low-frequency motion cues from aircraft motion kinematic parameters, enhancing simulation accuracy. Secondly, proposed method significantly expands the range of simulated motion cues while ensuring their synchronization with high-frequency motion cues along relevant degrees of freedom. This formulation of the problem increases the range of simulated motion cues to ±0,3nz, which practically corresponds to the range of simulated motion cues of a transport aircraft, and thus increases the quality of motion cueing. The implementation of the developed method on the An-72TK-200 full flight simulator confirmed its effectiveness. In conclusion, this study introduces a promising methodology that enhances the quality of motion cueing, thus rendering flight simulations more realistic and beneficial for both pilots and aerospace engineering researchers in the real of non-maneuvering aircraft flight simulators.

An enhanced Deep-Learning empowered Threat-Hunting Framework for software-defined Internet of Things

The Software-Defined Networking (SDN) powered Internet of Things (IoT) offers a global perspective of the network and facilitates control and access of IoT devices using a centralized high-level network approach called Software Defined-IoT (SD-IoT). However, this integration and high flow of data generated by IoT devices raises serious security issues in the centralized control intelligence of SD-IoT. Motivated by the aforementioned challenges, we present a new Deep-Learning empowered Threat Hunting Framework named DLTHF to protect SD-IoT data and detect (binary and multi-vector) attack vectors. First, an automated unsupervised feature extraction module is designed that combines data perturbation-driven encoding and normalization-driven scaling with the proposed Long Short-Term Memory Contractive Sparse AutoEncoder (LSTMCSAE) method to filter and transform dataset values into the protected format. Second, using the encoded data, a novel Threat Detection System (TDS) using Multi-head Self-attention-based Bidirectional Recurrent Neural Networks (MhSaBiGRNN) is designed to detect cyber threats and their types. In particular, a unique TDS strategy is developed in which each time instances is analyzed and allocated a self-learned weight based on the degree of relevance. Further, we also design a deployment architecture for DLTHF in the SD-IoT network. The framework is rigorously evaluated on two new SD-IoT data sources to show its effectiveness.

First investigation of RH gene polymorphism in patients with sickle cell disease and associated blood donors in Cameroon, Central Africa.

Although genetic polymorphism of the RH blood group system is well known in sub-Saharan Africa, national/regional specificities still remain to be described precisely. For the first time in Cameroon, Central Africa, and in order to better characterize the molecular basis driving RH phenotype variability, as well as to identify the main antigens that may be potentially responsible for alloimmunization, we sought 1) to study the RH genes in a cohort of 109 patients with sickle cell disease; 2) to study the same genes in the corresponding donors whose red blood cells (RBCs) were transfused to the patients (108 donors in 98 patients); 3) to predict RH phenotype on the basis of the molecular data and compare the results with serologic testing; and 4) to identify retrospectively patients at risk for alloimmunization. In order to generate an exhaustive dataset, the RH genes of all patient and donor samples were systematically investigated 1) by quantitative multiplex PCR of short fluorescent fragments (QMPSF) for characterization of RHD gene zygosity and potential structural variants (SVs), and 2) by Sanger sequencing for identification of single nucleotide variants (SNVs). Subsequent to molecular analysis, the genotypes and RH phenotype were deduced and predicted, respectively, from reference databases. In a total of 217 Cameroonian individuals, as many as 24 and up to 22 variant alleles were identified in the RHD and RHCE genes, respectively, in addition to the reference alleles. Interestingly, 65 patients with SCD (66.3%) were assumed to be exposed to one or more undesirable RH antigen(s) with varying degrees of clinical relevance. Beyond the comprehensive report of the nature and distribution of RH variant alleles in a subset of Cameroonian patients treated by transfusion therapy, this work highlights the need for an extensive review of current practice, including routine serologic typing procedures, preferably in the near future.