Degree Of Environmental Stress Research Articles

Comparative analyses of functional traits based on metabolome and economic traits variation of Bletilla striata: Contribution of intercropping.

The intercropping practice has been regarded as a practical land-use selection to improve the management benefits of Bletilla striata plantations. The reports about the variety of economic and functional traits of Bletilla pseudobulb under intercropping systems were limited. The present study investigated the variation of economic and functional traits of Bletilla pseudobulb under different intercropping systems (the deep-rooted intercropping system: B. striata - Cyclocarya paliurus, CB; and the shallow-rooted intercropping system: B. striata - Phyllostachys edulis, PB). The functional traits were analyzed through non-targeted metabolomics based on GC-MS. The results indicated that the PB intercropping system significantly decreased the yield of Bletilla pseudobulb while significantly increasing the total phenol and flavonoids compared with the control (CK). However, there were no significant differences in all economic traits between CB and CK. The functional traits among CB, PB, and CK were separated and exhibited significant differences. Under different intercropping systems, B. striata may adopt different functional strategies in response to interspecific competition. The functional node metabolites (D-galactose, cellobiose, raffinose, D-fructose, maltose, and D-ribose) were up-regulated in CB, while the functional node metabolites (L-valine, L-leucine, L-isoleucine, methionine, L-lysine, serine, D-glucose, cellobiose, trehalose, maltose, D-ribose, palatinose, raffinose, xylobiose, L-rhamnose, melezitose, and maltotriose) were up-regulated in PB. The correlation between economic and functional traits depends on the degree of environmental stress. Artificial neural network models (ANNs) accurately predicted the variation in economic traits via the combination of functional node metabolites in PB. The correlation analysis of environmental factors indicated that Ns (including TN, NH4 +-, and NO3 --), SRI (solar radiation intensity), and SOC were the main factors that affected the economic traits (yield, total phenol, and total flavonoids). TN, SRI, and SOC were the main factors affecting the functional traits of the Bletilla pseudobulb. These findings strengthen our understanding of the variation of economic and functional traits of Bletilla pseudobulb under intercropping and clarify the main limiting environmental factors under B. striata intercropping systems.

Accumulations of technogenic emissions in the snow cover of the north-west in the Kurgan region

The study of the accumulation and spatial distribution of ecotoxicants on agricultural lands is one of the important aspects in solving the problem related to the environmental safety of animal feed and food products obtained. In this regard, there is a need to assess the content of pollutants in the chain atmosphere – snow cover. The purpose of the research was to assess the level of contamination of the snow cover of agricultural enterprises in the areas of the north-west of the Kurgan region exposed to man-made impacts. In the north-western part of the Kurgan region three variants of territories were identifi ed that diff er in the degree of environmental stress. The fi rst territory is experiencing the load from the nearby powerful South Ural industrial agglomeration, subject to transboundary man–made pollution, spreading both by water and aerogenically (Kataysky area); the second territory is the subject to transboundary man-made pollution by enterprises of the Chelyabinsk region, and is the location of facilities for the storage and destruction of chemical weapons (Shchuchansky area); the third territory is the territory of relative ecological well–being (Shadrinsky and Kargapolsky areas). In the areas of the north-west of the Kurgan region, the zonal nature of technogenic pollution of snow cover and soils has been revealed. Pollution of the studied territories occurs through the introduction of technogenic aerosols, as well as the influence of local sources. It was revealed that nitrites, nitrates, ammonium nitrogen, manganese make up the largest share in the association of pollution of the studied territories, while sulfates, chlorides and zinc make up the smallest share. In the snow cover, the highest concentration of heavy metals was detected in the fi rst and second territories. The third territory is subject to contamination by the studied elements to a lesser extent.

Environmental protection measures in mineral resource development: case study of a gold-bearing deposit in the Russian Far East.

In the Russian mining industry, the recent social and economic processes inevitably affect environmental safety and the social security of all those affected by mining. Thus, this study aimed to evaluate the technogenic impacts of a mining company on the environment. Measures were developed and implemented to ensure ecological safety and social security during a mineral resource development project in the southern part of the Russian Far East. This study analysed global experiences in this regard and carried out field research with the aim of establishing an inventory of plants and animals (terrestrial and aquatic), showing that technogenesis produced new specific landforms, e.g. quarries and dumps that replaced natural landforms. The main ecologically negative impacts of the mining operations in the region were the movement of mountain masses, changes in forms of erosion, and destruction of mountain ranges with the formation of dispersed clastic fractions of large specific surface areas, which determine exomorphodynamic processes, e.g. deflation, suffusion, and landslides. A general assessment of the biota status and natural water quality within the boundaries of influence of the developed deposit was presented, and a set of measures was recommended for environmental protection and ensuring the rational use of natural resources during mining operations. Moreover, the necessity of creating effective mining and environmental monitoring systems was supported. A 'Map of the Ecological State of Gold Mining Development in the Albazino Territory' was compiled for the first time, pinpointing areas undergoing various degrees of environmental stress. Changes in the forested areas within the territory of the mining allotment were forecast using the forest cover of the study area as the baseline.

Habitat‐complexity regulates the intensity of facilitation along an environmental stress gradient

Positive interactions between foundation species and their associated species are expected to be influenced by the degree of environmental stress as well as trait variations of the species involved. However, there is scarce empirical evidence regarding how these two factors interact and shape the intensity of facilitation. To test how facilitation varies with stress, a colonization experiment using artificial algal units that varied in a functional trait (morphological complexity) was conducted at different intertidal height levels. The isolated effects of shore‐height and host‐complexity are as expected: the number of species and overall abundance of epifauna decreased with lower complexity host units and greater exposure to environmental stress. However, in contrast to the initially proposed model, based on the stress gradient hypothesis, the magnitude of positive interactions increased at lower tidal heights (those levels with, presumably, milder environmental conditions). An additional predation exclusion experiment ruled out the effects of predation pressure as a major factor driving the structure of mobile epifaunal assemblages in these intertidal habitats. Thus, further studies are needed to identify the factors that explain the patterns of facilitation intensity found in the present study.

Биохимический состав печени и мышц карповых рыб

Disorders in the liver are often accompanied by changes in its lipid composition. And since the liver performs many functions in the body, indicators of liver metabolic processes can reflect the living conditions and General condition of the fish body. The article provides information about some biochemical parameters of the liver and muscles of bream caught in the waters of the Volga Delta in different years. It seems that the causes of changes in the accumulation of lipids in the liver and muscles may be different factors (fish species, season, physiological state). Ultimately, the lipid content is determined by a number of processes. The complex of physiological and biochemical parameters proposed by the authors can be used to assess the degree of environmental stress on cyprinid fish.

Seasonality and Small Spatial-Scale Variation of Chlorophyll a Fluorescence in Bryophyte Syntrichia ruralis [Hedw.] in Semi-Arid Sandy Grassland, Hungary.

Bryophytes face challenges due to global climate change which is leading to in-depth research in monitoring and studying their photosynthetic activity. The aim of this preliminary experiment was to study the seasonal variation trend in the chlorophyll a fluorescence parameters, Fv/Fm (ratio of variable to maximum fluorescence), photochemical fluorescence quenching (qP), photochemical quantum yield of photosystem II (ΦPS II), fluorescence quenching (qN), and non-photochemical quenching (NPQ), in the moss cushions of Syntrichia ruralis [Hedw.] collected from semi-arid sandy dunes for two slopes i.e., north-east (NE) and south-west (SW) direction. Our results showed a seasonal and small-spatial scale variation trend in all chlorophyll fluorescence parameters. These variations are due to different seasonal conditions referring to different degrees of environmental stress. ΦPS II and qP values were maximum in winter and in spring seasons while Fv/Fm, NPQ and qN were maximum in summer. Based on the different exposition of dunes, the SW slope showed increased values of the effective quantum yield of PS II and qP in comparison to the NE slope due to the optimal microclimate conditions for their expansion. These results may refer to the future changing in diversification and coverage of the Syntrichia species in semi-arid sandy grassland due to more effective metabolism in the beneficial microclimatic conditions.

The Growth Characteristics of Three Terrestrial Plants Cultivated with Biogas Slurry as a Hydroponic Medium

Biogas slurry is a traditional high-quality organic liquid fertilizer. Three terrestrial plants with high economic value, fu gui cai, yang xin cai and strawberry, were selected for hydroponic cultivation using an optimal dilution of biogas slurry. The results of the experiments showed that strawberries could bloom and bear fruit and fu gui cai and yang xin cai grew well. However, in comparison with the control groups planted in soil, all three crops were subject to a certain degree of environmental stress, as shown by changes in growth, biomass indicators, physiological indicators and morphological indicators. The leaves and stems of yang cai and fu gui cai were tested for several types of heavy metals, and all met the requirements for human consumption. It is feasible to cultivate these three plants with biogas slurry. The results of this study can help guide hydroponic production practices and have practical significance and reference value.

Genetic rescue insights from population- and family-level hybridization effects in brook trout

Although hybridization can be used as a tool for genetic rescue, it can also generate outbreeding depression and reduce local adaptation. Improved understanding of these processes is required to better inform conservation decisions for threatened populations. Few studies, however, investigate how multiple factors influence hybridization effects. We investigated how effective population sizes (Ne), geographic distance, genetic divergence (QST, FST), and environmental stress influence hybridization effects among eight highly divergent brook trout (Salvelinus fontinalis) populations varying in Ne (range 44–589). In a common garden, we compared three fitness-related traits among hybrid and non-hybrid crosses. Contrary to expectations, we found little evidence of outbreeding depression; instead, hybridization effects were mostly neutral (60/66 non-hybrid vs. hybrid comparisons) with some support for heterosis (6/66). When controlling for maternal-family effects, several factors influenced fitness-related traits but cumulatively explained little variance in relative hybrid fitness (0–6.4%). For instance, when hybridized dams came from small Ne populations, relative fitness increased for some traits (length at hatch), suggesting heterosis, yet decreased at other traits (survival to hatch), suggesting outbreeding depression. Trait inconsistencies in relative hybrid fitness were also observed under different degrees of environmental stress. Results also differed when family variance in hybridization effects was unaccounted for. Collectively, our results suggest that, under certain conditions, current guidelines on genetic rescue and associated outbreeding risks might be too conservative. The occurrence of genetic rescue and outbreeding depression, nonetheless, remain difficult to predict because families and individual traits can express different effects from hybridization within populations.

On the association between stature sexual dimorphism and the nutritional status of men and women in the long run

height variations across cohorts are a proxy of the evolution of living conditions and, specifically, of the nutritional status of a given population. However, the interpretation of the changes in stature sexual dimorphism are controversial. to test the association between nutritional status and the changes in height differentials between men and women (sexual dimorphism) in the long run (19th and 20th centuries). three data sources containing measured adult heights are used, namely: - Data from previous works. - Data from health examination surveys in OECD countries. - Data from the NCD RisC Factor Collaboration project. Two indicators are analyzed: absolute sexual dimorphism (men's height minus women's height) and the ratio of sexual dimorphism (men's height divided by women's height). a secular trend of sexual dimorphism is evidenced over the second half of the 20th century coinciding with the substantial improvement of environmental factors that determine the net nutritional status. Among cohorts born at the end of the 20th century in non-marginal environments, the average sexual dimorphism was found to be 13.69 cm -absolute- and 1.084 -ratio-. In comparison with these modern figures of sexual dimorphism, those found among cohorts born during the 19th century are abnormally low, especially during periods of worsening of living conditions. if properly addressed, sexual dimorphism has the ability to report on the degree of environmental stress and its impact on the nutritional status and its differentials across specific groups of the population both cross-sectionally and over time.

Regional Differentiation and Classification for Carrying Constraints on the Resources and Environment of China

Abstract: Research on the relationship between national resource constraint-region types and environmental carrying capacity is essential for the continued development of Chinese industrialization and urbanization. Thus, utilizing a series of key indexes including the per-capita potential of available land resources, the per-capita potential of available water resources, the degree of environmental stress, and the degree of ecological restriction, a step-by-step, integrated measuring method is presented here to understand the constrained carrying elements of water and land resources as well as environment and ecology. Spatial differences are analyzed and area types classified at the county level across China. Results reveal that: (1) Almost 90% of China is strongly constrained by both resources and the environment, while nearly 50% of national territory is strongly constrained by two elements, especially in areas of intensive population and industry to the east of the Helan-Longmen Mountain line; (2) Dens...

Spatial fuzzy clustering approach to characterize flood risk in urban storm water drainage systems

Increasing environmental stress of drainage systems leads to frequent occurrence of urban flooding, which generates significant adverse economic, social, and environmental impacts. In this study, spatial fuzzy clustering approach (SFCA) is developed to estimate possible flood risks in storm water drainage systems and address complexity and uncertainties of flood risk assessment. The proposed approach is capable of gaining insights into system behavior by exploring spatial patterns of flood risk. The corresponding algorithm is utilized to divide a drainage system into various clusters to reflect flood risk levels along the network space. Application to a sample drainage system demonstrates that it provides an appropriate technique to determine spatial distribution of flood risks. Results reveal that areas with high, moderate, and low risk identified by SFCA correspond to a certain degree of environmental stress. The findings of this study can serve as a preliminary basis to guide managers in their evaluation of flood risks in various drainage management scenarios.

Associações de Foraminíferos em Resposta a Variações Ambientais da Laguna de Aveiro – Portugal

This study is based on the characterization of the sedimentary environment in selected sites, located in Aveiro canals and salt-pans, in the eastern-central and northern part of Aveiro Lagoon. Results of physicochemical parameters measured in water and sediment, as well as grain size, geochemical and microfaunal (benthic foraminifera) data are analyzed. The determinants factors of spatial and seasonal variability of living and dead foraminiferal assemblages were analyzed in two sampling periods, late summer and late winter, in order to study the response of these organisms to the impact caused by pollution and sazonal variation of physicochemical parameters. Biotic and abiotic results are analyzed to discriminate sites with different degree of environmental stress. The conditions of salinity, temperature, pH and Eh in the studied sites vary spatially and seasonally. The sediment in these locations is generally poorly oxygenated, suboxic or even anoxic a few millimeters below the surface. Some of the sites are affected by chemicals derived from industrial effluents and urban contaminants. The most polluted areas by heavy metals such as As, Cr, Cu and Zn are Porto de Salreu, Largo do Laranjo and some locals in the city of Aveiro. Foraminiferal assemblages in the studied sites integrate common lagoonal and estuarine euryhaline and eurythermic species, most of which tolerant to the oxygen reduction. The size of the living foraminiferal communities is reduced and their structure affected by increasing concentrations of heavy metals such as As, Cr, Cu and Zn, as well as by the high variability of environmental parameters such as low Eh, related to depressed levels of oxygen in the sediment, leading to the death of many species. The results also indicate a greater tolerance of some opportunistic species to the Pb enrichment, since the environment offer them abundance and quality of food.

Benthic foraminiferal assemblages in active volcanic area of the Azores Islands (North Atlantic Ocean)

The present study shows the preliminary micropalaeontological results obtained from surface samples collected during an Eurofleet marine geological survey in the Terceira offshore (Azores Archipelago, Portugal) carried out in September 2011.Benthic foraminiferal assemblages were studied in order to obtain a sea-floor ecological characterisation in natural stressed environments like active volcanic area of the Azores Islands. Sediments were collected at water depths ranging between 260 and 410 m and consist of blackish, sand-sized glass shards, in which highly vesicularto scoriaceous clasts prevail on fluidal striated fragments. All samples were stained with Rose Bengal to determine living and dead assemblages.The dominance of living specimens suggests that the first generation of foraminiferal colonisers was found. Diversity index (α-Fisher index), Faunal density and Foraminiferal Abnormality Index (FAI) were calculated to assess the structure of the assemblage and the degree of environmental stress. Living and dead assemblages are verysimilar; they are very scarce but sufficiently diversified. The living benthic assemblage is dominated by epi/shallow infaunal species Angulogerina angulosa and typical infaunal taxa like Bolivina spp.Subordinately other epifaunal (Ehrenbergina bradyi, Lenticulina spp., Quinqueloculina seminula, Stomatorbina concentrica) and agglutinated species (Ammoglobigerina globigeriniformis, Eggerelloides scabrus, Haplophragmoides canariensis, Spiroplectinella wrightii, Textularia spp.) are also recorded. Significant frequencies of specimens showing signs of test decalcification were found. These test alterations could be due to hydrothermal fluids, since similar features were recorded in foraminiferal specimens coming from other volcanic substrates in the Aeolian Arc (Tyrrhenian Sea). The dominance of living and dead A. angulosa specimens allows us to consider this species as opportunistic pioneer taxon able to colonise stressed environments. This is confirmed also by the highest values of FAI (>2%), recorded in most of the samples, indicating a persisting environmental stress. Angulogerina angulosa and Bolivina spp. are the species showing more abundant deformed specimens (irregular development of chambers and aberrant tests). On the basis of our observations, the recolonisation process and the spatial distribution patterns are strongly influenced by the sea bottom high hydrodynamic regime which is responsible for volcanic deposit transport and depositional events. These conditions may be reflected on the relationship between benthic foraminifers and substrate features, which are mainly restricted to grain size, morphology and texture of volcanic shards.

Responses of five Mediterranean halophytes to seasonal changes in environmental conditions.

In their natural habitats, different mechanisms may contribute to the tolerance of halophytes to high soil salinity and other abiotic stresses, but their relative contribution and ecological relevance, for a given species, remain largely unknown. We studied the responses to changing environmental conditions of five halophytes (Sarcocornia fruticosa, Inula crithmoides, Plantago crassifolia, Juncus maritimus and J. acutus) in a Mediterranean salt marsh, from summer 2009 to autumn 2010. A principal component analysis was used to correlate soil and climatic data with changes in the plants' contents of chemical markers associated with stress responses: ions, osmolytes, malondialdehyde (MDA, a marker of oxidative stress) and antioxidant systems. Stress tolerance in S. fruticosa, I. crithmoides and P. crassifolia (all succulent dicots) seemed to depend mostly on the transport of ions to aerial parts and the biosynthesis of specific osmolytes, whereas both Juncus species (monocots) were able to avoid accumulation of toxic ions, maintaining relatively high K(+)/Na(+) ratios. For the most salt-tolerant taxa (S. fruticosa and I. crithmoides), seasonal variations of Na(+), Cl(-), K(+) and glycine betaine, their major osmolyte, did not correlate with environmental parameters associated with salt or water stress, suggesting that their tolerance mechanisms are constitutive and relatively independent of external conditions, although they could be mediated by changes in the subcellular compartmentalization of ions and compatible osmolytes. Proline levels were too low in all the species to possibly have any effect on osmotic adjustment. However-except for P. crassifolia-proline may play a role in stress tolerance based on its 'osmoprotectant' functions. No correlation was observed between the degree of environmental stress and the levels of MDA or enzymatic and non-enzymatic antioxidants, indicating that the investigated halophytes are not subjected to oxidative stress under natural conditions and do not, therefore, need to activate antioxidant defence mechanisms.

Reconsidering locomotor habits and life style of the Balearic insular giant rodent Hypnomys Bate, 1918 from the allometry of the limb long bones

The genus Hypnomys Bate, 1918 includes some endemic Neogene chronospecies from Mallorca and Menorca, evolved in insularity conditions from the Lower Pliocene to the human arrival at the archipelago. The study of the allometric proportions (functional length and sagittal diameter) of the limbs’ long bones of Hypnomys eliomyoides Agusti, 1980 (Lower Pleistocene of Menorca), Hypnomys sp. (Pleistocene of Menorca), Hypnomys onicensis (Reumer, 1994) (Upper Pliocene-Lower Pleistocene of Mallorca) and Hypnomys morpheus Bate, 1918 (Upper Pleistocene of Mallorca) only indicates small differences with the garden dormouse (Eliomys quercinus Linnaeus, 1776) and other mainland rodents and insectivores. The study about the locomotion type by means of Similarity Matrix of Bou indicates that 1) Hypnomys spp. presents the greater similarities with the arboreal, jumping and gliding species; 2) the jumping and digging life style of Hypnomys spp. are more developed (25%) than in E. quercinus; 3) the walking locomotion of Hypnomys spp. is only a 7% greater than in E. quercinus; and 4) the degree of similarity with the gliding locomotion of Hypnomys sp. is greater (17%) than in E. quercinus. Some of this data indicates that Hypnomys spp. was better adapted to the arboreal life than E. quercinus, due to the relationship between the jumping and gliding capacities with the arboreal locomotion. The greater similarity of Hypnomys spp. with the walking locomotion does not necessarily involve more terrestrial habits. Our interpretation contrasts with earlier conclusions of Hypnomys life style (Hypnomys cf. onicensis and Hypnomys morpheus) where the terrestrial locomotion was overestimated in front of the arboreal locomotion. The locomotion type of Hypnomys would be related with the presence of important forest masses on the Balearic Islands during the Neogene, a different degree of environmental stress from that of the mainland ecosystems and a probable expansion of the ecological niche occupied by these species.

Physiological differences explain the co‐existence of different regeneration strategies in Mediterranean ecosystems

• Fire and drought are selective driving forces in Mediterranean plants, and thus their ability to resprout or recruit after these disturbances is of paramount importance. The contrast in regeneration niche between resprouters and seeders, and each group's different root characteristics, suggest that they are subjected to different degrees of environmental stress and, consequently, to different evolutionary forces. • We compared leaf traits, xylem traits related to hydraulic efficiency and vulnerability to cavitation, and the physiological response to an imposed drought between seedlings of resprouters and seedlings of seeders. We used 12 species co-existing in Mediterranean basin ecosystems. • Major differences were found in the xylem architecture and leaf traits, and in the response to drought conditions. Seeders were more efficient at transporting water to leaves but formed, in turn, a safer xylem network. They also presented higher photosynthesis and transpiration rates, and earlier stomatal closure with drought, but good leaf dehydration tolerance. • Seeders and resprouters can be considered two syndromes whose different functional characteristics are related to water availability and drought responses. These characteristics, together with their differences in rooting habits, account for their distinct regeneration niches and, thus, their co-existence.

Environmental-dependent proline accumulation in plants living on gypsum soils

Biosynthesis of proline—or other compatible solutes—is a conserved response of all organisms to different abiotic stress conditions leading to cellular dehydration. However, the biological relevance of this reaction for plant stress tolerance mechanisms remains largely unknown, since there are very few available data on proline levels in stress-tolerant plants under natural conditions. The aim of this work was to establish the relationship between proline levels and different environmental stress factors in plants living on gypsum soils. During the 2-year study (2009–2010), soil parameters and climatic data were monitored, and proline contents were determined, in six successive samplings, in ten taxa present in selected experimental plots, three in a gypsum area and one in a semiarid zone, both located in the province of Valencia, in south-east Spain. Mean proline values varied significantly between species; however, seasonal variations within species were in many cases even wider, with the most extreme differences registered in Helianthemum syriacum (almost 30 μmol g−1 of DW in summer 2009, as compared to ca. 0.5 in spring, in one of the plots of the gypsum zone). Higher proline contents in plants were generally observed under lower soil humidity conditions, especially in the 2009 summer sampling preceded by a severe drought period. Our results clearly show a positive correlation between the degree of environmental stress and the proline level in most of the taxa included in this study, supporting a functional role of proline in stress tolerance mechanisms of plants adapted to gypsum. However, the main trigger of proline biosynthesis in this type of habitat, as in arid or semiarid zones, is water deficit, while the component of ‘salt stress’ due to the presence of gypsum in the soil only plays a secondary role.

Effects of diking on the biological performance of the black mangrove (Avicennia germinans L.) in an Atlantic mangrove forest

The effects of diking on the photosynthetic rate, gross glucose production, biomass, and nutrient levels of the black mangrove Avicennia germinans L., were investigated during the major dry, the major wet and the intermediate season in completely diked mangrove areas, and compared to partially diked and non-diked biotopes. In the major dry and major wet seasons, the photosynthetic rate and gross glucose production were two to five times lower in the completely diked sites compared to the partially diked and non-diked habitats. The average dry weights of branches, flowers and leaves did not differ significantly between sites. The same held true for propagules from the major dry and major wet season. However, propagules from the transition period collected in the non-diked and partially diked biotopes yielded appreciable amounts of dry matter, while plants in the completely diked forests did not carry any propagules. No statistically significant differences were found among nutrient content in the leaves of plants from the different habitats. Our results suggest black mangrove growing in diked habitats may suffer a certain degree of environmental stress, which may affect important biological processes, such as photosynthesis, gross glucose production, and propagule formation.

Do interrelationships among benthic components mirror disturbance levels?

The hypothesis tested in this study is that changes in benthic ecosystem components interrelationships may mirror the degree of environmental stress in the Mediterranean coastal lagoons. Multivariate matrices deriving from four benthic components (macrophytes, zoobenthos, epibenthic decapods and demersal fish) from four lagoonal stations along a well-defined disturbance gradient were compared by means of second-stage non-metric multidimensional scaling (MDS). The resulting inter-matrix distances were used as a proxy for the identification of the degree of disturbance. The approach followed is novel in that it uses information from higher levels of the biological organization by taking into account more than a single benthic component, thus representing broad categories of functional groups. The second-stage MDS plots depict differences between inter-component distances in the sampling stations according to the degree of disturbance they experience and the BIOENV analysis demonstrates that certain components are correlated with the environmental variables at a higher degree in the most disturbed stations.

The fitness costs of developmental canalization and plasticity

Organisms are capable of an astonishing repertoire of phenotypic responses to the environment, and these often define important adaptive solutions to heterogeneous and unpredictable conditions. The terms 'phenotypic plasticity' and 'canalization' indicate whether environmental variation has a large or small effect on the phenotype. The evolution of canalization and plasticity is influenced by optimizing selection-targeting traits within environments, but inherent fitness costs of plasticity may also be important. We present a meta-analysis of 27 studies (of 16 species of plant and 7 animals) that have measured selection on the degree of plasticity independent of the characters expressed within environments. Costs of plasticity and canalization were equally frequent and usually mild; large costs were observed only in studies with low sample size. We tested the importance of several covariates, but only the degree of environmental stress was marginally positively related to the cost of plasticity. These findings suggest that costs of plasticity are often weak, and may influence phenotypic evolution only under stressful conditions.