As an indispensable part of rhizosphere microorganisms, fungi participate in the processes of soil and rhizosphere element cycle and the degradation of pollutants in soil through self metabolism, and have huge potential in protecting tomato from abiotic and biological stresses. Tomato is one of the main varieties in the global trade of vegetables and fruits. In recent years, the planting area of tomato has continued to expand. The simple and intensive planting combined with high temperature and wet environmental conditions has facilitated the growth and breeding of diseases and pests, which has led to a sharp decline in tomato production. In addition, excessive use of chemicals and fertilizers has led to environmental pollution, which has led to a decline in tomato quality, and has caused huge losses to China’s vegetable economy. In this experiment, the rhizosphere soil of several common tomato diseases was used as research materials, and ITS sequencing was carried out for rhizosphere soil fungi by using high-throughput sequencing analysis. The operational taxon units (OTUs), alpha diversity, beat diversity and community structure were analyzed.