Decreased Tear Production Research Articles

Corneal Epithelial Thickness in Sjogren's Disease: A Pilot Study.

This study was to assess corneal epithelial thickness (CET) in patients with Sjogren's disease (SjD). A retrospective chart review was conducted of SjD patients from September 2021 to January 2022. Patient demographics, unanesthetized Schirmer's test, serologic markers, and symptoms as measured by the Ocular Surface Disease Index (OSDI) were reviewed. Epithelial thickness from both eyes was measured using anterior segment OCT at the central 3mm and concentric 5mm, 7mm, and 9mm zones for the superior, temporal, inferior, and nasal corneal quadrants. Associations between corneal epithelial thickness with patient demographics, clinical characteristics, and symptoms were evaluated using regression models. Fifteen SjD patients (100% female) were included with a mean age of 58.4 years. Patients with Sjogren's disease had a significantly thinner superior corneal epithelium compared to the inferior epithelium (mean 47.7mm vs 53.1mm, p = 0.001). The epithelial thickness mean standard deviation (MSD) was significantly inversely correlated with the unanesthetized Schirmer test (r=-0.39, p = 0.005), suggesting that an overall variability of CET correlates with decreased aqueous tear production. SS-A, SS-B, ANA, and RF positivity were not associated with any measures of CET. This pilot study suggests that there is significant superior versus inferior thinning of corneal epithelium in Sjogren's patients. There was a significant correlation between variability of corneal epithelial thickness and decreased tear production in Sjogren's patients. Further larger studies are needed to understand the relationship of CET with objective and subjective measurements of ocular surface disease.

Topical naltrexone increases aquaporin 5 production in the lacrimal gland and restores tear production in diabetic rats.

Diabetes mellitus is a prevalent disease that is often accompanied by ocular surface abnormalities including delayed epithelial wound healing and decreased corneal sensitivity. The impact of diabetes on the lacrimal functional unit (LFU) and the structures responsible for maintaining tear homeostasis, is not completely known. It has been shown that the Opioid Growth Factor Receptor (OGFr), and its ligand, Opioid Growth Factor (OGF), is dysregulated in the ocular surface of diabetic rats leading to overproduction of the inhibitory growth peptide OGF. The opioid antagonist naltrexone hydrochloride (NTX) blocks the OGF-OGFr pathway, and complete blockade following systemic or topical treatment with NTX restores the rate of re-epithelialization of corneal epithelial wounds, normalizes corneal sensitivity, and reverses dry eye in diabetic animal models. These effects occur rapidly and within days of initiating treatment. The present study was designed to understand mechanisms related to the fast reversal (<5 days) of dry eye by NTX in type 1 diabetes (T1D) by investigating dysregulation of the LFU. The approach involved examination of the morphology of the LFU before and after NTX treatment. Male and female adult Sprague-Dawley rats were rendered hyperglycemic with streptozotocin, and after 6 weeks rats were considered to be a T1D model. Rats received topical NTX twice daily to one eye for 10 days. During the period of treatment, tear production and corneal sensitivity were recorded. On day 11, animals were euthanized and orbital tissues including conjunctiva, eyelids, and lacrimal glands, were removed and processed for histologic examination including immunohistochemistry. Male and female T1D rats had significantly decreased tear production and corneal insensitivity, significantly decreased number and size of lacrimal gland acini, decreased expression of aquaporin-5 (AQP5) protein and decreased goblet cell size. Thus, 10 days of NTX treatment restored tear production and corneal sensitivity to normal values, increased AQP5 expression, and restored the surface area of goblet cells to normal. NTX had no effect on the number of lacrimal gland acini or the number of conjunctival goblet cells. In summary, blockade of the OGF-OGFr pathway with NTX reversed corneal and lacrimal gland complications and restored some components of tear homeostasis confirming the efficacy of topical NTX as a treatment for ocular defects in diabetes.

Prevalence of dry eye disease among IT students in Pakistan.

Dry eye disease (DED) is a common tear film disorder caused by decreased tear production or increased tear evaporation. The increased use of computers in the workplace has brought about the development of ocular health concerns, including dry eye disease. Therefore, this study was designed to determine the prevalence of DED associated with computer work and recommend preventing or reducing its development. This study investigated the prevalence of DED among IT students in Dera Ghazi Khan. A cross-sectional descriptive study was conducted among 327 undergraduate students of the IT department in the district of Dera Ghazi khan over 5months from September 2021 to January 2022. DED was assessed subjectively with Ocular Surface Disease Index. Data were analysed using SPSS version 21. The quantitative analysis was presented as mean and standard deviation, and qualitative variables were introduced in frequency and percentages. The chi-square test (Fisher's exact test) was used to study the significance of associations between dependent and independent variables. The mean age of the participants was 20.25 ± 1.305years (range 17-25). The prevalence of DED among computer programmers was 47.7%. This study showed that gender (p = 0.31), contact lens usage (p = 0.64), and duration of time spent on computer display units (p = 0.47) were not significant determinants of DED among computer programmers. DED is prevalent among IT students, along with different symptoms. However, further studies are required to adequately measure the prevalence of DED during longer exposure time to computer screens and explore environmental risk factors.

Consumption of Limosilactobacillus fermentum Inhibits Corneal Damage and Inflammation in Dry Eye Disease Mouse Model through Regulating the Gut Microbiome.

The present study investigated the effect of orally administered Limosilactobacillus fermentum HY7302 (HY7302) on the relationship between ocular tissue and the microbiome in a corneal injury dry eye mouse model. Specifically, 0.1% benzalkonium chloride (BAC) was applied to the ocular surface for 14 days to induce corneal injury in male Balb/c mice. During the BAC treatment period, HY7302 (1 × 108 CFU/kg/day or 1 × 109 CFU/kg/day) or an omega-3 positive control (400 mg/kg/day) were administered orally (n = eight/group). To examine the signaling pathways affected by the HY7302 treatment, the in vitro effects of HY7302 on the tight junctions and the inflammatory response were investigated in the mouse colon epithelial cell line, CMT-93. BAC exposure decreased tear production, induced ocular inflammation and corneal epithelial detachment, and altered the gut microbiota. However, oral administration of HY7302 restored tear secretion and decreased corneal epithelial detachment in BAC-treated corneal injury mice. Further, HY7302 alleviated corneal inflammation via modulation of matrix metalloproteinase-9 (MMP-9) expression and affeted alterations in gut microbiota composition. These findings suggest that the gut-eye axis interaction between gut microbiota and corneal tissue affects disease severity in corneal injury, and that the alteration of the microbiota by HY7302 could improve eye health by regulating the inflammatory response.

Topical netarsudil for the treatment of primary corneal endothelial degeneration in dogs

This study evaluated the tolerability and efficacy of the topical rho-kinase inhibitor netarsudil for canine primary corneal endothelial degeneration (PCED). Twenty-six eyes of 21 client-owned dogs with PCED were enrolled in a prospective, randomized, vehicle control clinical trial and received topical netarsudil 0.02% (Rhopressa®) or vehicle control twice daily (BID) for the first 4 months. Then, all patients received netarsudil for the next 4 or 8 months. Complete ophthalmic examination, ultrasonic pachymetry, Fourier-domain optical coherence tomography, and in vivo confocal microscopy were performed at baseline and 1, 2, 4, 6, 8 and 12 months. Effect of netarsudil on central corneal thickness (CCT), percentage of cornea with edema, and endothelial cell density (ECD) were evaluated by repeated measures ANOVA. Kaplan–Meier curves and log-rank test were used to compare corneal edema and clinical progression of eyes in netarsudil versus vehicle control groups. All dogs developed conjunctival hyperemia in at least one eye while receiving netarsudil. Unilateral transient reticulated intraepithelial bullae and stromal hemorrhage were observed respectively in 2 dogs in the netarsudil group. Two dogs showed persistently decreased tear production while receiving netarsudil, requiring topical immunomodulatory treatment. No significant differences in CCT, ECD, corneal edema or clinical progression were observed between netarsudil or vehicle treated eyes. When comparing efficacy of topical netarsudil BID and topical ripasudil 0.4% administered four times daily from our previous study, dogs receiving ripasudil had significantly less progression than those receiving netarsudil.

Ameliorative effects of Tagetes erecta Linn. flower against desiccation stress-induced dry eye symptoms in the mice model

BackgroundTagetes erecta Linn, popularly known as Marigold, has various pharmacological effects. It is used as a dietary supplement, especially for the posterior segment of the eye. However, the effect of T. erecta Linn on ocular disorders is still unknown. The purpose of this study was to investigate the effect of oral administration of ethanol extract of T. erecta Linn flower (TE) for dry eye syndrome (DED) in a murine model. MethodsTwenty-four mice were subjected to desiccation stress (DS) to induce DED and subcutaneous injection of scopolamine hydrobromide was administered 4 times a day for 21 days. TE and cyclosporine A (CsA) were administered for an additional 14 days under DS conditions. Mice were randomly divided into four groups: control, TE200, TE400, and CsA. Changes in tear production and corneal fluorescein staining were measured at baseline, after 7 days of DS, and after treatment for 7 and 14 days. ResultsDS significantly decreased tear production and increased corneal fluorescein score; the parameters were significantly reversed in the TE400 (oral administration of 400 mg TE/kg body weight) group. TE markedly improved DS-induced changes including corneal epithelial detachment and lacrimal gland inflammation. The anti-inflammatory effect of TE 400 supplementation was similar to that of CsA. ConclusionsOur findings suggest that oral administration of TE may protect against DS-induced DED via stabilization of the tear film and suppression of inflammation. This study provides an experimental basis for further studies on the potential clinical use of TE in preventing DED.

Rethinking Sjögren Beyond Inflammation: Considering the Role of Nerves in Driving Disease Manifestations.

Sjögren syndrome (SS) is a chronic inflammatory autoimmune disease characterized by destruction of mucosal glands resulting in dry eye and dry mouth. Ocular presentations can be heterogenous in SS with corneal nerves abnormalities that are structural, functional, or both. Some individuals present with corneal hyposensitivity, with a phenotype of decreased tear production and epithelial disruption. Others present with corneal hypersensitivity, with a phenotype of neuropathic pain including light sensitivity and pain out of proportion to signs of tear dysfunction. A similar correlate can be found outside the eye, with dry mouth predominating in some individuals while pain conditions predominate in others. Understanding how nerve status affects SS phenotype is an important first step to improving disease management by targeting nerve abnormalities, as well as inflammation.

How does ocular graft-versus-host disease fit under the dry eye umbrella? A review.

Graft-versus-host disease (GVHD) is a systemic disease that can affect multiple organs as a consequence of an allogeneic haematopoietic stem cell transplant. One organ system that is often affected in GVHD is the eyes. Ocular GVHD (oGVHD) may involve various structures within the eye including the lacrimal glands, eyelids, conjunctiva, cornea, and nasolacrimal ducts, and is a source of morbidity in patients with GVHD. Common presenting features of GVHD overlap with dry eye disease (DED), including decreased tear production, epithelial disruption, and Meibomian gland dysfunction (MGD). In this review, we aim to compare oGVHD and DED to better understand the similarities and differences between the conditions, with a focus on pathophysiology, risk factors, clinical features, and treatments.

Measuring corneal pain in the clinic

DED is a heterogenous disease with a variety of symptoms and signs. One reality of the disease is that its symptoms (e.g., ocular surface pain described as “dryness”, “irritation”, “aching”, and/or “tenderness”) often do not align with its signs (e.g., decreased tear production, tear instability). New data suggest that corneal nerve abnormalities underlie the noted discordance between symptoms and signs. DED symptoms out of proportion to signs, as is often seen co‐morbid with pain conditions such as migraine and fibromyalgia, may indicate that neuropathic mechanisms contribute to symptoms. DED signs out of proportion to symptoms, as is often seen co‐morbid with diabetes mellitus, may indicate that neurotrophic mechanisms are involved. Given this reality, testing for corneal nerve function is an important, but often overlooked, component of the ocular surface examination. This presentation will review strategies to test corneal nerve function in the clinical arena, and discuss treatments that can be used when corneal nerve abnormalities are detected.

Bony Congenital Nasolacrimal Duct Obstruction: A Novel Phenotype of Aplasia of Lacrimal and Major Salivary Glands

PurposeAplasia of lacrimal and salivary gland (ALSG) is a syndromic disorder characterized by aplasia of lacrimal and salivary systems. Reported ophthalmic manifestations of ALSG include aplasia of lacrimal glands, punctal agenesis, lacrimal sac mucocele and membranous congenital nasolacrimal duct obstruction (CNLDO). Bony CNLDO, a rare cause of congenital epiphora, has not been associated with any syndromic disorder. This study investigated the relationship between genetic mutations and bony CNLDO in three Chinese families with ALSG. DesignSingle-center observational case study. ParticipantsThree Chinese families with bony CNLDO including 7 affected and 9 healthy family members. MethodsSlit-lamp ophthalmic examination, comprehensive physical examination, orbital Computed Tomography (CT), cervicofacial Magnetic Resonance Imaging (MRI), audiometry and whole exome sequencing on periphery blood were performed. Variants were cross-referenced with 1000 control genomes and various population databases. Pathologic variants were identified using bioinformatic tools. Main Outcome MeasuresClinical examination, diagnostic imaging, whole exome sequencing and bioinformatic analysis findings. ResultsAffected patients had decreased tear production on Schimer I Test and reduced tear break-up time. Bony CNLDO was observed on computed tomography, showing unilateral or bilateral bony termination at the middle or terminal segment of the nasolacrimal canal. Cervicofacial MRI showed aplasia or absence of lacrimal, parotid and submandibular glands. Physical examination revealed normal ears, digits and facial morphology. Audiometry and dental assessment were conducted on the pediatric patients and yielded normal results. The clinical characteristic of patients aligned with diagnosis of ALSG. Genomic analysis revealed three novel heterozygous missense mutations of Fgf10 gene: c.316T>C, c.327C>G, c.332T>G. The inheritance pattern was autosomal dominant with variable penetrance. These variants were not observed in 1000 control chromosomes and population databases. These variant positions were also shown to be highly conversed across various animal species. Mutated genes and proteins were predicted as “deleterious” with most computational models with a few suggesting they may be “benign”. ConclusionsBony CNLDO was identified as a novel phenotype of ALSG implicated by missense mutations of highly conserved residues in the Fgf10 gene. These cases broadened our knowledge of Fgf10-related phenotypes and prompt clinicians to consider syndromic associations in patients with bony CNLDO.

Investigating the Blink Reflex Abnormality in Dry Eye Patients.

Introduction Dry eye is an ocular surface disorder caused by increased evaporation, decreased tear production, or mixed form. Tears are secreted by the lacrimal gland after lacrimal nerve stimulation connected to the facial nerve.In nerve damage, tear secretion decreases, and dry eye develops. Our aim is to investigate the presence of pathology in the facialtrigeminal nerveand neuronal pathways that provide reflex connections between these nerves by measuring the blink reflex in patients with dry eyes. Methods Schirmer test and tear breakup time were performed. Tear breakup time measurement was repeated three times, and the average of three was accepted. Tear breakup time<10 secondsand Schirmer test <10 mm without local anesthesia were accepted as dry eye. Patients having traumatic corneal pathology, ectatic corneal disease, inflammatory and microbial keratitis, previous ocular surgery, glaucoma, diabetic retinopathy, and chronic neurological diseases were excluded. The control group was randomly formed from 42 eyes of 21 healthy volunteers. Blink reflex was measured in both groups, and the R1 and R2 responses of the two groups were compared. Written consent was obtained from the patient (or legal guardian) so that her medical data could be published. Results There was no significant difference between the two groups in R1 and R2 responses in both eyes. There was no significant difference in terms of gender between the two groups (p=0.100). The mean age in the patient group was significantly higher than in the control group (p<0.000).The mean Schirmer test in the patient group was 8.6±1.1 mm in the right eye and 8.97±1.0 mm in the left eye. Conclusion There was no central pathology observed in terms of reflex blinking in dry eye disease.However, in future studies, brainstem fiesta sequence magnetic resonance imaging (MRI) can be planned to evaluate central pathologies in more detail.

Diminished pupillary light reflexes, elevated third eyelids, and decreased tear production are commonly associated with canine dysautonomia.

To retrospectively describe ocular abnormalities reported in dogs with presumed dysautonomia. 79 dogs with dysautonomia. Medical records from the Kansas State University Veterinary Health Center from 2004 to 2021 were reviewed for dogs with a clinical or histopathologic diagnosis of canine dysautonomia (CD). Ophthalmic exam abnormalities, nonocular clinical signs, and outcomes were recorded. Most dogs (73/79 [92.4%]) with CD exhibited at least 1 ocular abnormality. The most common ocular abnormalities were diminished pupillary light reflexes (PLRs) in 55 of 79 (69.6%) dogs and elevation of the third eyelids in 51 of 79 (64.6%) dogs. Schirmer tear test values were bilaterally decreased in 32 of 56 (57.1%) dogs. Other ocular abnormalities included resting mydriasis, ocular discharge, photophobia, blepharospasm, corneal ulceration, and conjunctival vessel pallor. The most common nonocular clinical signs were vomiting or regurgitation in 69 of 79 (87.3%) and diarrhea in 34 of 79 (43.0%) dogs. Pharmacologic testing with dilute 0.01%, 0.05%, or 0.1% pilocarpine yielded pupillary constriction in 42 of 51 (82.4%) dogs. Thirty-two of 79 (40.5%) dogs survived to discharge. Resolution of ocular abnormalities was variable. Ophthalmic abnormalities such as diminished PLRs, elevation of the third eyelids, and decreased tear production are commonly associated with CD and provide support for its antemortem clinical diagnosis, though dogs with normal PLRs can be diagnosed with the disease. Pharmacologic testing with dilute topical pilocarpine in dogs with clinical signs suggestive of dysautonomia supports a diagnosis of CD. Ophthalmic abnormalities may improve or resolve over time.

Mass cytometry reveals the corneal immune cell changes at single cell level in diabetic mice.

Diabetic ocular complications include sight-threatening consequences and decreased corneal sensitivity, characterized by decreased tear production, corneal sensitivity and delayed corneal epithelial wound healing. The pathogenesis of diabetic corneal disorders remains largely unknown. Growing evidence implies the participation of immune cells in the development of diabetic corneal diseases. Nonetheless, the immunological changes that result in diabetic corneal problems are largely unknown. Mass cytometry by time of flight (CyTOF) was used to investigate immune cell cluster alterations associated with diabetic corneal disorders. CyTOF test was performed on corneal cells at a single level from 21-week-old diabetic (db/db) and non-diabetic (db/m) mice. A panel of 41 immune-related markers monitored different immune cell types in diabetic corneas. To investigate the proportion of each immune cell subpopulation, an unsupervised clustering method was employed, and T-distributed stochastic neighbor embedding was used to visualize the distinctions between different immune cell subsets. Through CyTOF test, we identified 10 immune cell subsets in the corneal tissues. In a novel way, we discovered significant immune alterations in diabetic corneas, including pronounced alterations in T cells and myeloid cell subgroups in diabetic corneas linked to potential biomarkers, including CD103, CCR2, SiglecF, Ly6G, and CD172a. Comprehensive immunological profiling indicated remarkable changes in the immune microenvironment in diabetic corneas, characterized by a notable decrease in CD103+CD8+ tissue-resident memory T (TRM) cells and Tregs, as well as a dramatic increase of γδT cells and subsets of CD11b+Ly6G+ myeloid-derived suppressor cells (MDSCs). CyTOF analysis revealed significant alterations in the immune microenvironment during the development of diabetic corneal complications. This study mapped the immune microenvironment landscape of type 2 diabetic corneas, providing a fundamental understanding of immune-driven diabetic corneal disorders.

Bilateral corneal ulceration in a cat after general anaesthesia

AbstractGeneral anaesthesia is accompanied by a loss of the blink reflex, decreased tear production, inability to close the eye and decreased stability of the tear film. Protection of the eye with corneal lubricant is standard during general anaesthesia, but clear recommendations are missing. Moreover, the anaesthetic event may trigger other factors that increase susceptibility to corneal ulceration. In this case report, we describe a clinical case of bilateral corneal ulceration in a cat following general anaesthesia. We discuss how our practice may be improved, and examine the possibility that feline herpes virus resurgence during veterinary care may be a risk factor for corneal lesions.

Study of correlation between vitamin D3 levels and dry eye.

Dry eye is a prevalent disorder of tear film resulting from either decreased tear production or increased tear evaporation. It is becoming a serious issue due to its disturbing symptoms, which become progressively troublesome affecting the work efficiency of patients and increasing financial burden due to lifelong dependency on eye drops. If not detected early, it can lead to sight-threatening complications. This study aims to explore serum vitamin D3 deficiency as a causative factor of dry eye. The study was conducted in the outpatient department of a tertiary care hospital in India, for a period of two years from September 2018 to September 2020. About 40 patients who had dry eye and 20 controls were enrolled in this study. They were given an Ocular Surface Disease Index (OSDI) questionnaire, examined for signs of dry eye on slit lamp with Schirmer's test and tear film break-up time. All 60 participants were subjected to serum vitamin D3 level laboratory test and its deficiency prevalence was correlated with dry eye and its severity. Serum vitamin D3 deficiency was found to be more prevalent in patients with dry eye. There was no gender predilection or change in prevalence with increasing age. Vitamin D3 level was negatively correlated with OSDI and positively with Schirmer's test 1 and 2 and tear film break-up time (TBUT) scores. Conclusion: The prevalence of vitamin D3 deficiency was not consistently found to be associated with the increasing severity of dry eye.

Aim2 Deficiency Ameliorates Lacrimal Gland Destruction and Corneal Epithelium Defects in an Experimental Dry Eye Model.

Dry eye disease (DED) is a multifactorial disease that is associated with inflammation. Excessive DNA is present in the tear fluid of patients with DED. Absent in melanoma 2 (AIM2) is a key DNA sensor. This study aimed to investigate the role of AIM2 in the pathogenesis of DED. DED was induced by injection of scopolamine (SCOP). Aberrant DNA was detected by cell-free DNA (cfDNA) ELISA and immunostaining. Corneal epithelial defects were assessed by corneal fluorescein staining, zonula occludens-1 immunostaining and TUNEL. Tear production was analyzed by phenol red thread test. Lacrimal gland (LG) histology was evaluated by hematoxylin and eosin staining, and transmission electron microscopy examination. Macrophage infiltration in LG was detected by immunohistochemistry for the macrophage marker F4/80. Gene expression was analyzed by RT-qPCR. Protein production was examined by immunoblot analysis or ELISA. Aim2-/- mice displayed a normal structure and function of LG and cornea under normal conditions. In SCOP-induced DED, wild type (WT) mice showed increased cfDNA in tear fluid, and aberrant accumulations of dsDNA accompanied by increased AIM2 expression in the LG. In SCOP-induced DED, WT mice displayed damaged structures of LG, reduced tear production, and severe corneal epithelium defects, whereas Aim2-/- mice had a better preserved LG structure, less decreased tear production, and improved clinical signs of dry eye. Furthermore, genetic deletion of Aim2 suppressed the increased infiltration of macrophages and inhibited N-GSDMD and IL18 production in the LG of SCOP-induced DED. Aim2 deficiency alleviates ocular surface damage and LG inflammation in SCOP-induced DED.

Expression of Androgen and Estrogen Receptors in the Human Lacrimal Gland

Lacrimal gland dysfunction causes dry eye disease (DED) due to decreased tear production. Aqueous-deficient DED is more prevalent in women, suggesting that sexual dimorphism of the human lacrimal gland could be a potential cause. Sex steroid hormones are a key factor in the development of sexual dimorphism. This study aimed to quantify estrogen receptor (ER) and androgen receptor (AR) expression in the human lacrimal gland and compare it between sexes. RNA was isolated from 35 human lacrimal gland tissue samples collected from 19 cornea donors. AR, ERα, and ERβ mRNA was identified in all samples, and their expression was quantified using qPCR. Immunohistochemical staining was performed on selected samples to evaluate protein expression of the receptors. ERα mRNA expression was significantly higher than the expression of AR and ERβ. No difference in sex steroid hormone (SSH) receptor mRNA expression was observed between sexes, and no correlation was observed with age. If ERα protein expression is found to be concordant with mRNA expression, it should be investigated further as a potential target for hormone therapy of DED. Further research is needed to elucidate the role of sex steroid hormone receptors in sex-related differences of lacrimal gland structure and disease.

INCIDENCE AND RISK FACTORS FOR DRY EYE DISEASE IN A TERTIARY HOSPITAL IN NORTH CENTRAL INDIA

Objectives: Dry eye disease (DED is a severe tear insufficiency condition that affects millions of people worldwide. Dry eye refers to conditions of the tear film brought on by decreased tear production and/or excessive tear evaporation. The study’s objective was to compile data on the incidence of DED and associated risk factors. Methods: The government hospital of Uttar Pradesh (India) served as the study’s site for this cross-sectional and observational study. Clinical observation and the ocular surface disease index survey were used to assess the aim of the study. Results: Patients were screened in total, 652. The incidence of DED was reported in 267 (40.9%) individuals. There were 130 DED patients (48.7%) above the age of 40, followed by those between the ages of 21 and 40 (47.2%). Both genders were equally impacted, with men (50.2%) and women (49.2%). Out of 267 DED patients, 128 (47.9%) had a desk job with a computer. Among the patients, 57 (21.3%) had mild DED, 74 (27.7%) had moderate DED, and 136 (50.9%) had severe DED. The severe DED also showed independent association between the desk job with a computer (Odds Ratio [OR]; 2.782, 95% confidence interval [CI]: 1.694–4.568, p&lt;0.001), cigarette smoking (OR; 1.849, 95% CI: 1.135–3.014, p=0.014), and use of contact lens (OR; 1.972, 95% CI: 1.206–3.223, p=0.007). Conclusion: The frequency of DED is high in Uttar Pradesh. The illness is particularly prevalent in older people, computer-using desk workers, smokers, and contact lens wearers.

Dry eye syndrome treatment with autologous blood serum eye drops: literature review

Ieva Kunickaitė1 1Vilnius University, Faculty of Medicine, Vilnius, Lithuania Abstract Introduction. Dry eye syndrome (DES) is a tear film disorder caused by decreased tear production or instability of the tear film. Prevalence of DES ranges from 5% to 87.5% worldwide. The most common recommendation for the treatment of DES is the use of preservative-free artificial […]

Canine ophthalmic patients with endocrine and metabolic disorders - a review

The eye is particularly sensitive to various pathological processes in the body. Ocular changes are often the first symptoms of serious systemic diseases. Due to hormonal and metabolic disturbances in patients with endocrinopathies and metabolic disorders, secondary ophthalmic diseases can occur. Systemic diseases often cause ocular problems in animal and human patients. Early diagnosis of ophthalmic symptoms can help to identify a primary cause, such as endocrinopathies, and start an effective treatment. In some cases, clinical signs of systemic diseases are poorly documented in veterinary ophthalmology. Cataracts, retinopathy, metabolic disorders and systemic hypertension are common problems in animals with diabetes mellitus. Patients with hyperadrenocorticism may develop complications secondary to hyperlipidaemia and hypertension. Acute blindness due to sudden retinal dege­ne­ration (SARDS) has been associated with pituitary-adrenal axis disorders. Growth hormone disorders can result in secondary ocular complications due to hypertension (corneal infiltrates, decreased tear production and neurological dysfunction). Animals with hyperthyroidism may have ophthalmic problems associated with systemic hypertension and subsequent retinal bleeding or detachment.