Decreased Feed Consumption Research Articles

First detection of D181 genotype of infectious bronchitis in poultry flocks of Morocco

BackgroundThis paper reports the first pathological and molecular characterization of the novel variant of infectious bronchitis virus (IBV) D181 in poultry flocks in Morocco and Africa.MethodsThe study includes six poultry farms, involving three flocks of layers aged between 28 and 67 weeks and three broiler flocks aged 27, 39 and 42 days from different regions of Morocco. In all affected layer flocks, a severe drop in egg production with poor eggshell quality was reported. Necropsy of dead birds was carried out, and samples of trachea, lungs, oviduct, ovaries, and kidneys were fixed in 10% neutral buffered formalin for histopathologic examinations, while other portions were stored at -20 °C for molecular analysis. Real time RT-qPCR for IBV gene group was performed, and IBV variants were identified. Partial S1 gene sequences were amplified by conventional RT-PCR, sequenced, and aligned for phylogenetic and amino acid similarity analysis.ResultsNecropsy of dead birds revealed misshapen and hemorrhagic ovarian follicles with an edematous oviduct and severe reaction in the cecal tonsils. A caseous material accumulation in the sinus was noted in few birds. In contrast, the broiler flocks exhibited respiratory clinical signs such as difficulty in breathing, sneezing, tracheal rales, watery eyes and lethargy, associated with a decrease in feed consumption. Mortality in broiler ranged from 2 to 15%. Histopathological analysis of samples showed a lympho-plasmocytic inflammation in the oviduct, trachea, and lungs. Individual necrosis of epithelial cells, with sloughing of the bronchial epithelium and accumulation of desquamated cells with mucus in the airways, was observed in some birds. Partial S1 gene sequencing and phylogenetic analyses showed that the Moroccan strains were very closely related to D181 strains isolated in Dutch layers and breeders in 2018. Nucleotide sequence identities reached 90.9–95% with the Dutch isolates (strain CK/NL/D181/2018).ConclusionOur sequencing results demonstrate for the first time that the D181 IBV genotype is circulating in Moroccan poultry. These findings justify permanent monitoring of circulating strains in order to appropriately adjust vaccination strategies to align with the evolving field situation.

The Effect of Feeding Formula in Honey Bees (Apis mellifera) Using Flow Hive Technology on Honey Production and Quality at PT Kembang Joyo Sriwijaya

Bee feeding has long been known and applied in honey bee farming. One way of feeding bees is to give sugar syrup mixed with water. This study aims to determine the effect of supplementary feeding on bees that have been formulated on the production and quality of honey produced. This study also applies one of the artificial hive technologies, namely flow hive. This study also observed how the adaptation of bees and the added value of the flow hive using Apis mellifera honey bees which is known for its high production rate and high adaptability. This research was conducted from September to December 2023 at PT Kembang Joyo Sriwijaya, Malang Regency. The experiment was conducted using 3 flow hives with the same feed formula. The study showed good feed consumption in stup 1 and decreased feed consumption in stup 2 and stup 3. Honey obtained in stup 1 was 1.5 kg from 1 flow frame. Laboratory test results showed pH levels of 3.4, moisture content of 18.2 %, reducing sugar levels of 55.44 % and 56.71 % and diastase enzyme activity of 3.18 DN and 3.19 DN. In conclusion that formula feeding did not show high honey production when compared to the time taken to reach harvest and also did not show fulfilling reducing sugar content and diastase enzyme activity test results.

Effect of mineral feed additive on egg productivity of laying hens

Improving the consumption and increasing the efficiency of the use of feed, obtaining maximum egg productivity is ensured by a high level of balanced feeding with various feed additives. Therefore, the research work aimed to study the effect of mineral feed additives on the egg productivity of laying hens of the Loman Brown cross. During the experiment, the control group of laying hens consumed the basic ration (BR) – complete combined feed. In feeding the research group, a mineral selenium-containing feed additive was used with the main diet. Selenium in the feed additive is contained in organic form, selenomethionine and selenocystin. Unlike inorganic forms, selenoamino acids can be transported and metabolized in the body to build amino acids. According to the results of the experiment, it was found that when feeding mineral feed additive, there was an increase in the gross collection of eggs in laying hens of the 2nd group by 12.7 % (Р ≥ 0.01) and a decrease in feed consumption by 10 pcs. eggs by 9.0 %, relative to the control group. In addition, there is a tendency to increase the laying rate per average laying hen by 9.5 % and the laying intensity by 3.9 %. With the additional use of the mineral feed additive by laying hens of the 2nd group, the mass of eggs increases by 5.3 % (Р < 0.05), the height of the yolk by 25.0 % (Р < 0.05), and the yolk index by 0.06 % (P < 0.01) versus the control value. It was established that the use of mineral supplements by laying hens of the 2nd group increases live weight by 5.1 % (Р ≤ 0.05), absolute gain by 12.3 % (Р ≤ 0.01), pre-slaughter live weight by 5.7 % (Р ≤ 0.05), the weight of the semi-cartridge by 4.3 % (Р ≤ 0.05) and the weight of the carcass by 11.5 % (Р ≤ 0.01) against the reference indicator. Under the influence of the mineral feed additive in the 2nd group of laying hens, the survival rate of the flock increased by 3.0 % compared to the control analogs.

Utilization of Marine Fungi as a Feed Additive on the Performance of Local Chickens’ Digestive Organs

This study aimed to evaluate the utilization of marine fungi as feed additives on the performance of local chickens' digestive organs. This study used one hundred and eighty local chickens aged one day which were kept for 10 weeks and divided into five treatment groups and four replications. Chickens consumed treatment diets containing yellow maize, rice bran, soybean meal, fish meal, coconut meal, L-lysine, methionine, CaCO3, and added marine fungi. Chickens were slaughtered at 10 weeks old and digestive organs (liver, ventriculus, and small intestine) were collected and weighed to measure digestive organs' performance. Data were analyzed by analysis of variance by a completely randomized design. An orthogonal contrast test analyzed the significant effects of treatment diets on the parameters. This study showed that marine fungi significantly decreased feed consumption (P<0.05) without any declining digestive organ performance. It is suggested to add marine fungi extract to the local chicken diet as much as a 2 ml/kg ration.

Uji Daya Koksidiostat Ekstrak Temulawak (Curcuma xanthorrhiza) Asal Pulau Timor pada Ayam Buras

The development of livestock business in Indonesia has very profitable business prospects because the demand for animal products continues to grow. One of the livestock sub-sectors that are most in demand is poultry farming, especially free-range chicken. However, the development of poultry farming does not escape the obstacles faced by farmers, namely diseases, coccidiosis is one of them, which is a gastrointestinal protozoan infection caused by Eimeria spp. Coccidiosis management currently uses a coccidiostat, one of which is sulfaquinoxalin. However, sulfaquinoxalin has the disadvantage that it can cause a decrease in eggshell thickness and a decrease in feed consumption. The aim of this research is to find alternatives for the prevention and treatment of Eimeria tenella infection. This study used extracts of temulawak (Curcuma xanthorrhiza) from Timor Island. This study aims to know whether temulawak (Curcuma xanthorrhiza) extract from Timor Island is effective in treating coccidiosis and at what concentration is the most effective. The research methods included the manufacture of temulawak extract, experimental infection, collection of faecal samples, effectiveness testing and macroscopic observation of the cecum from chickens and calculating the score of the cecum lesions. This study used 3 treatment groups with 1 control. The treatment group used graded doses of temulawak (Curcuma xanthorrhiza) extract namely 0.2%, 0.4% and 0.6%, while the positive control used Coxy (©Medion) at a dose of 5 grams per liter of drinking water. The results showed that the temulawak extract (Curcuma xanthorrhiza) from the island of Timor was effective in inhibiting the growth of Eimeria tenella in vivo, with the most effective concentration of 0.2%.

Dietary Effects of Some Plant Extracts on Laying Performance, Egg Quality, and Some Blood Parameters in Laying Hens at Different Cage Densities.

This study was carried out to determine the effects of cage density and anise extract (AE), thyme extract (TE), and black cumin extract (BCE) supplementation in the diet of laying hens on laying performance, egg quality, and some blood parameters. A total of 288 Lohman White commercial hens were blocked according to the location of their cages. The four dietary treatments included a control, basal diet + 250 mg/kg of AE, basal diet + 250 mg/kg of BCE, and basal diet + 250 mg/kg of TE for 12 weeks. The cage density affected egg production (p < 0.05), feed consumption (p < 0.01), and cracked eggs (p < 0.05). Increasing the cage density caused a linear decrease in egg production and feed consumption. Compared to the control, there was a decrease in feed consumption (p < 0.01) in the plant extract groups, and in parallel, egg production decreased. An increased cage density did not affect the egg quality traits except the shell strength. The shell strength, yolk color, yolk index, albumen index, and Haught unit were significantly affected by the plant extracts. The cage density and plant extracts had a significant effect on the serum corticosterone and glucose (p < 0.01). The highest values of corticosterone and glucose were recorded for dietary TE with a cage density of 4 birds/cm2. On the other hand, the lowest values of these parameters were recorded for AE addition with a cage density of 3 birds/cm2. As a result, an increased cage density was associated with stress and depression in the feed consumption and metabolic profiles. Supplemental AE, BCE, and TE improved the laying performance and metabolic profiles.

Determination of the feed additive “Activo” effect on the productivity of lactating sows and young piglets under paratypic factors

The research was carried out at the pig farm of LLC Barkom, village Dublyany of Sambirsky district, Lviv region, and PE “Chernyuk” from Hrushvytsia Persha, Rivne district, Rivne region. According to the principle of analogs, two groups of farrowing sows of the PIC genetic company of the large white breed (2–3 farrowing) of 5 animals each were formed – control and trial. The animals were kept in the same house under the same conditions. The trial group received a standard diet similar to the control group, but the feed additive “Activo” was added to the diet in 0.1 kg/t of finished feed. From the fifth day after birth until weaning, newborn piglets of the trial group were fed pre-starter feed, to which 0.2 kg of the feed additive “Active” was added. The environmental parameters in the houses where the animals were kept differed according to the following leading indicators: the air temperature (°C) in the box with manual control of the supply-exhaust ventilation system in 5-, 14-, and 28-day-old piglets were, respectively, by 3; 2 and 4 °C higher compared to the temperature regime provided by the Big Duchman microclimate system; air movement speed (m/s) lower by 21.4; 33.3 and 8.3 %, respectively; carbon dioxide concentration (ppm) higher in 1.9; 1.8 and 2.1 times, respectively; ammonia concentration (mg/m3) higher in 4.5; 6.1 and 6.1 times, respectively; hydrogen sulfide concentration (mg/m3) higher in 1.8; 4 and 5.4 times, respectively. During the trial period, a probable tendency to decrease feed consumption by sows of the control group by 3.4 % (Р &lt; 0.01) was noted at LLC “Barkom”, and at PE “Chernyuk”, it amounted to 6.22 % (Р &lt; 0.001). At the same time, the loss of live weight in sows of the control group amounted to 10 % at the end of the period at LLC Barcom in the control group; namely, the sows of the group (K) lost an average of 23 kg during the trial period. The sows of the group (E) lost 17 kg. At the PE “Chernyuk” pig farm, the loss of live weight in sows at the end of the period in the control group was 25.8 kg, and in the trial group 19.4 kg, (Р &lt; 0.001), that is 24.8% less compared to the control group. At the pig complex of LLC "Barkom," when applying the feed additive “Activo” to lactating sows, an increase in the live weight of piglets in the trial group from birth to the fifth day after birth was noted by 18.3 %, compared to the control group. A significant increase in the live weight of piglets in the trial group was 17.16 % on the 14th day and 13.76 % (Р &lt; 0.001) on the 28th day of life, respectively. When calculating the average daily growth from birth to weaning (28 days of piglets' life), the difference was 18.54 % (Р &lt; 0.001). Piglets born from sows of the control group at the PE “Chernyuk” pig complex that did not consume the trial feed additive had a slightly lower live weight at birth by 9.4 % compared to the trial group. On the fifth day of life, the live weight of the piglets in the trial group was significantly greater than 30.2 % (Р &lt; 0.001). The tendency to increase the piglet's live weight on the 14th and 28th day of life was observed in the trial group by 20.1 % (Р &lt; 0.01) and 14.1 % (Р &lt; 0.001), respectively, in the control group. When calculating the average daily weight gain from birth to weaning (28 days of piglets' life), the difference was 15.15 % (Р &lt; 0.001).

PSX-18 Determining the Optimal Macroenvironment Temperature to Improve Lactating Sow and Piglet Productivity

Abstract Pre-weaned piglets prefer higher temperatures than lactating sows. To address this, swine producers use heating lamps or pads in combination with maintaining farrowing room macroenvironment temperatures at the upper end of the thermoneutral zone (24-25℃) of the lactating sow. This may inadvertently cause thermal stress for lactating sows resulting in decreased feed consumption, and ultimately, reduced milk production and litter growth. Therefore, the objective was to determine the sow level macroenvironment temperature that would improve both sow and litter productivity. In two repetitions, 37 lactating sows (parity = 3.1 ± 1.7) and their litters (d 1 litter size = 12.0 ± 1.0) were housed within 1 of 3 farrowing rooms set to different macroenvironment temperatures: LOW (n = 12 sows and litters; 15.0 ± 0.4℃), MID (n = 13 sows and litters; 19.3 ± 0.2℃), and HIGH (n = 12 sows and litters; 24.6 ± 0.8℃). Lactating sows and their litters were on trial from the onset of farrowing until weaning (19.8 ± 0.9 d of age). Individual farrowing crate temperatures were recorded throughout the trial in 0.14 s intervals using thermocouples mounted at sow head height. Piglets were provided a 0.34 x 1.52 m heating pad with a verified steady state of 35.0℃. Sow body weight (BW) was recorded at 24 h post-farrowing and at weaning, and piglet weights were measured on d 1, 7, 14 and at weaning to determine average daily BW gain (ADG). Liveborn piglet mortality was recorded throughout the trial. Sow feed intake (FI) was recorded daily from d 1 post-farrowing until weaning. The sow was considered the experimental unit for sow level measures and the litter was considered the experimental unit for piglet measures. Data were analyzed using PROC GLIMMIX in SAS 9.4 and using GLM in JMP 15. Regression models were used to determine the optimal macroenvironment temperature to improve sow FI and piglet ADG. Sow FI was greater (P &amp;lt; 0.01) for LOW (7.91 ± 0.38 kg) and MID (7.32 ± 0.37 kg) versus HIGH (6.37 ± 0.39 kg) sows, but similar for LOW and MID sows. Piglet ADG was greater from d 1 to weaning (P &amp;lt; 0.01) for LOW (0.25 ± 0.01 kg) and MID (0.26 ± 0.01 kg) versus HIGH (0.21 ± 0.01 kg) piglets, but similar for LOW and MID piglets. Pre-weaning mortality was similar (P = 0.32; 15.6%) among all treatments. A quadratic effect was observed (P &amp;lt; 0.01) where sow FI was maximized at a macroenvironment temperature of 17.2℃. A cubic effect was observed (P &amp;lt; 0.01) where piglet ADG was greatest at 17.0℃. In summary, these data have implications towards housing lactating sows and their litters at lower macroenvironment temperatures to improve growth performance.

Effects of Virgin Coconut Oil-Enriched Diet on Immune and Antioxidant Enzymatic Activity, Fat and Vitellogenin Contents in Newly Emerged and Forager Bees (Apis mellifera L.) Reared in Cages.

Searching for artificial diets positively affecting the survival, immune and antioxidant systems of honey bees is one of main challenges occurring in beekeeping. Among nutrients, lipids play a significant role in insect nutrition as structural components in cell membranes, energy sources and reserves, and are involved in many physiological processes. In this context, the aim of this work was to investigate the effect of 0.5% and 1% coconut oil-enriched diet administration on newly emerged and forager bees survival rate, feed intake, immune system, antioxidant system and both fat and vitellogenin content. In newly emerged bees, supplementation with 1% coconut oil determined a decrease in feed consumption, an increase in survival rate from the 3rd to 14th day of feeding, a short-term decrease in phenoloxidase activity, an increase in body fat and no differences in vitellogenin content. Conversely, supplementation with 0.5% coconut oil determined an increase in survival rate from the 3rd to 15th day of feeding and an increase in fat content in the long term (i.e., 20 days). Regarding the forager bee diet, enrichment with 0.5% and 1% coconut oil only determined an increase in fat content. Therefore, supplementation with coconut oil in honey bee diets at low percentages (0.5 and 1%) determines fat gain. Further investigations to evaluate the use of such supplement foods to prevent the fat loss of weak families during winter are desirable.

Penambahan ekstrak buah Mengkudu (Morinda citrifolia) dalam air minum terhadap performa broiler

Mengkudu fruit contains antioxidant substances to increase endurance, increase appetite, and reduce fat. This study aims to determine the effect of using noni fruit extract (Morinda Citrifolia) to improve broiler performance. The study used a completely randomized design (CRD) with 4 treatments and 4 replicates of 5 birds each. Treatments based on the concentration of mengkudu extract (EM) consisted of P0: control, P1: 10% EM, P2 12.5% EM, and P3: 15% EM. The treatment was given when the DOC was 1 day old until 28 days old. The research parameters were feed consumption, body weight gain, and feed conversion. The results showed that the use of noni extract in drinking water had no effect on broiler performance, body weight gain, and feed conversion. The use of mengkudu extract in drinking water from the level of 10 to 15% decreased feed consumption, increased body weight gain, and decreased broiler feed conversion significantly compared to the control. The addition of mengkudu extract in drinking water up to 15% improved broiler performance.

The effects of ad - libitum consumption of chitosan oligosaccharide with drinking water on laying quails

The aim of this study was to determine the effects of chitosan oligosaccharide on feed conversion ratio, some egg quality parameters, and some blood parameters in Japanise quails. Chitosan oligosaccharide (ChitO) was added to the drinking water of quails. A total of 192 (7 – 8 weeks old) Japanese quails (Coturnix coturnix Japonica) were divided into one control and 3 treatment groups, each consisting of 48 quails. Each group was divided into 6 replicates. While no additive was added to the control group, ChitO was added to the drinking water of the first, second, and third groups at the levels of 0.015, 0.030 and 0.045 g / L, respectively. They were given water and feed ad libitum. At the end of the study, no significant differences were observed between the control and treatment groups, except in their feed consumption and egg shell weight. In the first treatment group (given 0.015 g / L ChitO), it was observed that feed consumption decreased significantly compared to the control group; however, the difference between the other treatment groups was insignificant. The egg shell weight of the second treatment group (0.030 g / L ChitO) was found to be significantly higher (p &lt; 0.05) than that of the control group. In general, consumption of chitosan oligosaccharide with drinking water tended to decrease feed consumption, without adversely affecting feed efficiency, and tended to increase eggshell weight.

The European Green Deal improves the sustainability of food systems but has uneven economic impacts on consumers and farmers

The European Green Deal aims notably to achieve a fair, healthy, and environmentally friendly food system in the European Union. We develop a partial equilibrium economic model to assess the market and non-market impacts of the three main levers of the Green Deal targeting the food chain: reducing the use of chemical inputs in agriculture, decreasing post-harvest losses, and shifting toward healthier average diets containing lower quantities of animal-based products. Substantially improving the climate, biodiversity, and nutrition performance of the European food system requires jointly using the three levers. This allows a 20% reduction in greenhouse gas emissions of food consumption and a 40–50% decrease in biodiversity damage. Consumers win economically thanks to lower food expenditures. Livestock producers lose through quantity and price declines. Impacts on revenues of food/feed field crop producers are positive only when the increase in food consumption products outweighs the decrease in feed consumption.

INFLUENCE OF COUMARIN ON PRODUCTIVITY, IMMUNITY AND ANTIOXIDANT STATUS OF HEALTHY BROILERS

Background. Coumarins are natural compounds (secondary metabolites of plant origin) that have a wide range of biological activity, mainly due to their ability to interact with various enzymes and receptors in living organisms. The relevance of their use in feeding poultry is obvious, in view of the active search for alternatives to feed antibiotics.&#x0D; The aim of the study was to evaluate the effect of coumarin on the productivity and antioxidant status of broiler chickens.&#x0D; Materials and methods. Objects of study: broiler chickens of the Arbor Acres cross, 7-hydroxycoumarin. The experiment was carried out on 180 heads of 7-day-old broiler chickens (n=45). Control - the main diet (OR), 1 experimental – OR + coumarin (at a dose of 1 mg/kg of feed/day, 2 experimental – OR + coumarin at a dose of 2 mg/kg of feed / day, 3 experimental – OR + coumarin in at a dose of 3 mg/kg feed/day. Hematological parameters (number and type of leukocytes) were taken into account on an automatic hematological analyzer URIT-2900 Vet Plus (URIT Medical Electronic Group Co., Ltd, China). Biochemical analysis of blood serum was performed on an automatic biochemical analyzer CS-T240 (Dirui Industrial Co., Ltd, China).&#x0D; Results. The inclusion of 7-hydroxycoumarin in the diet contributed to an increase in live weight in the experimental groups by 8,6–19,4%; (by 9,2–21,1%), slaughter yield (up to 4,76%), against the background of a decrease in feed consumption per 1 kg of live weight gain (by 3,5–15,7%). showed a decrease in total protein, albumin, ALT (groups 1 and 2 (p≤0,05)), bilirubin (by 38,3–68,6%), cholesterol (in group 3 by 16,4% (p≤0,05)), triglycerides (groups 1 and 2, p≤0,05), urea (41,8–65,1%; p≤0,05), against the background of elevated iron levels (groups 1 and 2, p≤0,05). Depending on the dose of 7-hydroxycoumarin, a decrease in the number of leukocytes (group 1, p≤0,05), neutrophils (groups 1 and 2, p≤0,05), monocytes, eosinophils and basophils (p≤ 0,05). Antioxidant indicators were characterized by a decrease in the level of malondialdehyde (by 63,7–77,3%), superoxide dismutase activity (22,4–71,5%) and an increase in catalase activity (by 24,3–46,1%).&#x0D; Conclusion. Thus, the inclusion of 7-hydroxycoumarin (2 mg/kg feed/day) in the diet contributed to an increase in the live weight of broiler chickens, feed intake, slaughter yield, against the background of a lower feed consumption per 1 kg of live weight gain; had a positive effect on the parameters of the liver (ALT, total bilirubin), lipid and nitrogen metabolism (triglycerides, urea), the antioxidant status of the body of broiler chickens.

Effect of supplementing cellulase, carnitine and lemuru fish oil in feed on chemical composition of Muscovy duck meat

Muscovy duck in Indonesia has good potential to be developed as a meat producer, but currently its production and performance are still relatively low. additional feed is needed to increase it, such as cellulase enzymes, L-carnitine, and lemuru fish oil. where this combination, enzymes can increase nutrient digestibility, fish oil which is rich in unsaturated fatty acids and L-carnitine as a metabolic facilitator needed to oxidize long chain fatty acids. This research was conducted to investigate the effect of supplementing cellulase, carnitine and fish oil in feed on performance, EPA and DHA composition of Muscovy duck meat. In total, 120 Muscovy ducks in grower period were allocated to 4 dietary treatments with 5 replicates of 6 ducks. The treatments consisted of T0 = basal ration, T1 = T0 supplemented with 0.1% cellulase, T2 = T1 supplemented with L-carnitine 40 ppm, and T3 = T2 supplemented with 4% lemuru fish oil. The dietary treatments were fed for 90 days. The results of study showed that the effects of supplementing fish oil and L-carnitine in rations containing cellulase enzyme have a significant value (P<0.01) on the performance (feed consumption, daily body weight gain, feed conversion and IOFC) of male Muscovy duck grower phase and have a significant value (P<0.01) on EPA and DHA level in Muscovy duck meat. The supplementation can decrease feed consumption, meanwhile it increase daily body weight gain, improve feed conversion ratio, increase IOFC value, EPA and DHA level.

Fish Meal Substitution with Black Soldier Fly Larva on Growth Performance of Female Grower Quail

The study aimed to evaluate fish meal substitution with black soldier fly larvae supplemented with 2% Trichoderma culture on female grower performance and carcass traits quail. The materials used were 200 two-week-old female quails with an average body weight of 51.5 ± 7.3 grams. The study used a completely randomized design with five treatments and four replications. The treatments were: BSF0 (100% fish meal), BSF25 (75% fish meal + 25% BSF meal), BSF50 (50% fish meal + 50% BSF meal), BSF75 (25% fish meal + 75% BSF meal) ), BSF100 (100% BSF meal). The BSF meal used was added with 2% Trichoderma culture. The rations were iso-calorie and iso-protein, formulated from yellow corn, soybean meal, fish meal, BSF meal, mineral mix, and Trichoderma culture, with metabolic energy of 2,900 kcal/kg and 24% protein. The data were analyzed using variance analysis to determine if it had a significant effect, then continued with Duncan's Multiple Range Test. The results showed that the treatments were insignificant to the FCR, percentages carcass, and ovaries. Nevertheless, significant to (p&lt;0.05) feed intake, live weight, average daily gain and abdominal fat. The substitution of fish meal with BSF + 2% Trichoderma culture was more than 75% decreased feed consumption which caused a decrease in quail's life weight. According to the carcass traits, the BSF + Trichoderma sp 2% could be used up to 100%. However, based on quail performance, it can be concluded that BSF + 2% Trichoderma sp can be used up to 75% in quail rations without harmful effects.

Improved biological value of eggs due to the addition of pomegranate seed oil to laying-hen diets.

In this study, the effects of the addition of pomegranate seed oil (PSO) at different levels (0, 0.5, 1, and 1.5 ) to laying-hen rations on performance values, egg quality criteria, egg shelf life, some enzyme activity, and the fatty acid composition of yolks were investigated. In the study, 96 Lohman LSL laying hens at 64weeks of age were used. The trial consisted of four groups, each containing 24 hens. Chickens were given feed and water ad libitum during the 8-week experiment. The first group was the control group and was fed with a basal diet, while the other groups were fed with feeds with 0.5, 1.0, and 1.5 PSO added to the basal feed, respectively. The lowest feed consumption and the highest egg weight were determined in the 1 PSO group. The highest feed conversion ratio, the lowest eggshell weight, and the shell-breaking strength were determined in the 0.5 PSO group. It was determined that the egg yolk malondialdehyde (MDA) value in the groups to which pomegranate seed oil was added was significantly lower than the control group on the 28th day of storage. The lowest glutation (GSH) and catalase values were found in the control group, and the highest total antioxidant capacity (TAC) was found in the 1 PSO group. It was determined that the addition of PSO to the diet significantly increased the rate of saturated fatty acids (SEFA), conjugated linoleic acid (CLA), and conjugated linolenic acids (CLnA) in yolk. The results showed that the addition of 1 pomegranate seed oil to the feeds decreased feed consumption, increased egg weight, and positively affected the shelf life of the egg. In short, the addition of PSO had a positive effect on shelf life, and it increased punicic acid and CLA levels without reducing egg quality.

Campylobacter hepaticus in the Production Environment and Stagnant Water as a Potential Source of C. hepaticus Causing Spotty Liver Disease in Free-Range Laying Hens in Georgia, United States.

Spotty liver disease (SLD) has emerged as an important cause of disease in egg-producing flocks in countries such as the United Kingdom and Australia and has emerged in the United States. The organisms implicated in SLD include Campylobacter hepaticus and, more recently, Campylobacter bilis. These organisms have been found to cause focal lesions on the livers of infected birds. Campylobacter hepaticus infection results in reduced egg production, decreased feed consumption resulting in reduced egg size, and increased mortality of highly valuable hens. In the fall of 2021, birds from two flocks (A and B) of organic pasture-raised laying hens were submitted to the Poultry Diagnostic Research Center at the University of Georgia with a history suspicious of SLD. Postmortem examination of Flock A found 5/6 hens had small multifocal lesions on the liver and were PCR positive for C. hepaticus from pooled swab analysis of samples of the liver and gall bladder. Necropsy of Flock B found 6/7 submitted birds had spotty liver lesions. In pooled bile swabs, 2/7 hens from Flock B were also PCR positive for C. hepaticus. A follow-up visit to Flock A was scheduled 5 days later, as well as a visit to a flock where SLD has not been reported (Flock C), which was used as a comparative control. Samples of the liver, spleen, cecal tonsil, ceca, blood, and gall bladder were collected from six hens per house. Additionally, feed, water nipples, and environmental water (stagnant water outside the house) were collected from the affected farm and the control farm. To detect the organism, all samples collected were subjected to direct plating on blood agar and enrichment in Preston broth with incubation under microaerophilic conditions. After multiple phases of bacterial culture purification from all samples, single bacterial cultures displaying characteristics of C. hepaticus were tested by PCR to confirm identity. From Flock A, liver, ceca, cecal tonsils, gall bladder, and environmental water were PCR positive for C. hepaticus. No positive samples were detected in Flock C. After another follow-up visit, 10 wk later, Flock A was PCR positive for C. hepaticus from gall bladder bile and feces and one environmental water sample displayed a weak positive reaction for C. hepaticus. Flock C was PCR negative for C. hepaticus. To gain more knowledge about C. hepaticus prevalence, a survey of 6 layer hens from 12 different layer hen flocks between the ages of 7 to 80 wk, raised in different housing systems, were tested for C. hepaticus. The 12 layer hen flocks were culture and PCR negative for C. hepaticus. Currently, there are no approved treatments for C. hepaticus and no vaccine is available. The results of this study suggest that C. hepaticus may be endemic in some areas of the United States, and free-range laying hens may be exposed from the environment/stagnant water in areas where they range.

Yumurta Tavuklarında Sıcaklık Stresinin Verim ve Üretkenlik Üzerine Etkileri ile Alınacak Önlemler

High temperatures in hot regions and summer months impair the yield and productivity of laying hens. In this study, it is aimed to give detailed information about the measures to be taken to reduce the losses to the effects of heat stress on the yield and productivity of laying hens. Heat stress is one of the most challenging problems that affect all parameters of production performance and productivity, leading to high mortality rates and significant economic losses in commercial laying hens’ production due to inhibition of immune responses. It manifests itself with neurological symptoms such as high body temperature, hot-dry skin, paralysis, headache and loss of consciousness. It causes death as a result of heat cramps and stroke. In addition, heat stress disrupts the reproductive hormones of female-male laying hens. It causes infertility in male by reducing the number and movement of viable sperm. The effect of heat stress depends on age, sex, body weight, relative humidity and length of stay at high temperature. The temperature should not exceed 24°C for optimum egg yield and quality, and 27°C for welfare and productivity. Above this temperature, rapid breathing, the problem of not emitting heat from their bodies to the environment, decrease in feed consumption and live weight gain begin to be seen in chickens. At high temperatures, 34-35°C, egg production decreases by approximately 30% and feed intake decreases by 30-50%. As a result, measures such as feed management against heat stress, adding heat stress reducing additives to feed and water, climatic environmental control in shelters, early life conditioning and genetic selection of breeds with increased capacity of coping with heat stress conditions can be taken.

Diet-Independent Positive Effects of a Multi-Species Probiotic on the Growth Performance and Resistance against Vibrio parahaemolyticus in White Leg Shrimp.

Probiotic feed additives can support the gut health of shrimp and thereby improve performance, production efficiency and disease resistance. Two experiments in white leg shrimp aimed to investigate the effects of a multi-species probiotic feed supplement (AquaStar®, 3 g/kg feed, Biomin GmbH, Getzersdorf, Austria) in feed formulations with different marine meal levels (32% and 15%) on growth performance and resistance against Vibrio parahaemolyticus. Juvenile shrimp were stocked in a recirculating aquaculture tank system at a density of 20 shrimp/46.8 L and were fed diets with and without the probiotic supplementation for 8 weeks. Afterwards, a bath immersion with V. parahaemolyticus was performed and mortality was observed over a period of 14 days. Independent of the diet formulation, probiotic supplementation significantly improved the survival rate of the shrimp and the specific growth rate while decreasing feed consumption and feed conversion ratio when compared to the control (p ≤ 0.042). After the Vibrio immersion challenge, mortality was significantly decreased by 13.33% with probiotic supplementation in the high marine meal diet experiment (p = 0.042) and numerically decreased by 11.67% in the low marine meal diet experiment (p = 0.133). Overall, the results suggest that the beneficial effects of the probiotic can occur independently of the diet formulation.

НОВЫЙ ОТЕЧЕСТВЕННЫЙ АДСОРБЕНТ В БРОЙЛЕРНОМ ПТИЦЕВОДСТВЕ

As our research showed, the use of the new mycotoxin adsorbent SynergySorb®Detox-myco developed by Belarusian scientists, made it possible to reduce the toxic load on the body of broiler chickens. It was found that the introduction of the feed additive SynergySorb®Detox-myco into the diet of broiler chickens of the “Ross-308” cross in various concentrations contributed to an increase in the average live weight of broilers – by 0.1-1.3%, average daily gains – by 0.5-1.1%, livestock safety – 95.0-100% with a decrease in feed consumption per unit of production – by 1.7%. The achieved results make it possible to recommend the adsorbent SynergySorb Detox-myco for wide appli-cation in poultry farming for the prevention of mycotoxicoses and losses in poultry production.