Decrease In Na Research Articles

Suratentorial Tümör Cerrahisinde %20 Mannitol ve %3 Hipertonik Salin Kullanımlarının Beyin Relaksasyonu, Hemodinamik Parametreler, Serum Osmolaritesi ve Elektrolitleri Üzerine Etkilerinin Değerlendirilmesi

Objective: Different drugs or methods can be applied to provide brain relaxation in supratentorial tumor surgery. In our study, we aimed to evaluate effects of 20% Mannitol and 3% Hypertonic Saline (HS) used during supratentorial tumor surgery on brain relaxation, hemodynamic parameters, urine flow, fluid volume, pulse pressure variability (PPV), pleth variability index (PVI), serum osmolarity and electrolytes. Methods: The study included 60 patients aged 18-65 years, ASA I-II class, who were scheduled to undergo supratentorial tumor surgery. General anesthesia was administered with sevoflurane and remifentanil to achieve a state entropy of 40-60. Patients were allocated into two groups, with Group Mannitol receiving 20% mannitol (2.5 mL kg-1) and Group Hypertonic Saline receiving 3% HS (2.5 mL kg-1). In all patients, PPV and PVI values were planned to be ≤13%. After the dura was opened, brain relaxation scores were evaluated. Perioperative hemodynamic variables, blood gases, PVI, PPV values were recorded. Na+, K+ values, urine volume and administered fluid were recorded at 30, 45 and 60 minutes and 2-3 hours after the end of the infusion. Preoperative and postoperative BUN, creatinine and osmolarity values were recorded. Results: No statistical difference was found between demographic characteristics, brain relaxation and hemodynamic data. In HS group, PVI and PPV values were higher than Mannitol group from the beginning of infusion. In mannitol group, urine and administered fluid amounts were higher. Decrease in Na+ values was detected with Mannitol and increase with HS. It was determined that BUN and creatinine values were similar, serum osmolarity did not change at postoperative period in mannitol group and increased HS. Average intensive care and length of hospital stay were similar. Conclusion: In supratentorial tumor surgery, effective brain relaxation was achieved with Mannitol and HS, without affect to hemodynamics. Pulse pressure variability and PVI decreased with Mannitol. Blood gases, Na+, K+, BUN creatinine, osmolarity levels did not show serious changes. It was concluded that HS could be an alternative to mannitol in supratentorial surgery. Keywords: Hypertonic saline,mannitol, serum osmolarity, supratentorial tumor surgery

Influence of Anticholinesterase Drugs on Activity and Properties of Na+,K+-ATPase in Rat Erythrocytes under Stress Caused by Intense Physical Exercise.

We studied the effect of intramuscular injection of physostigmine and neostigmine on Na+,K+-ATPase activity in erythrocytes of rats subjected to intense physical exercise. Both anticholinesterase drugs had a significant effect on the development of the stress response, which was expressed in a decrease in the manifestation of its individual components such as the concentration of ascorbic acid in the adrenal glands, stress-related erythrocyte polycythemia, and LPO indicators. Anticholinesterase drugs reverse the stress-induced decrease in Na+,K+-ATPase activity, as well as changes in its magnesium-dependent properties. There were no changes in the activity of the studied enzyme in the erythrocyte ghosts. We associate the observed differences with the correction of the functions of the cholinergic components of the hypothalamic-pituitary-adrenal axis leading to the development of a hypoergic type stress reaction.

Effects of Kinetin and Gibberellic Acid on Growth and Ion Transport of Rice Under Cadmium Stress

An experiment was conducted to analyze the effects of kinetin and gibberellic acid on growth, and ion transport of rice (Oryza sativa var. BRRI-80) under cadmium stress. Exogenous application of both kinetin (2 µM) and gibberellic acid (1 µM) alleviated the negative effects of cadmium (Cd) on growth parameters by enhancing the length of both root and shoot, and biomass of Cd stressed rice plants grown on sand culture. Kinetin and GA3 caused decrease in Na+ accumulation while increasing K+ accumulation in both root and shoot. Accumulation of NO3- and PO43- were improved under cadmium stress by kinetin and GA3 in cadmium stressed rice plants. In addition to this, kinetin and GA3 reduced Cd accumulation in the root and shoot of cadmium stressed rice plants. Both kinetin and gibberellic acid were found effective in improving relative water contents in shoots of cadmium stressed rice plants. Bangladesh J. Bot. 53(2): 209-216, 2024 (June)

Age and composition of perovskite in ultramafic lamprophyres from the Zima alkaline-ultramafic carbonatite complex, the southern margin of the Siberian craton: Petrogenetic implications

This paper presents data on the age and trace element composition of perovskites from dykes of ultramafic lamprophyres (aillikites) of the Zima alkaline-ultramafic carbonatite complex (Bolshaya Tagna and Bushkanay) located within the Urik-Iya graben, Eastern Sayan region, southern margin of the Siberian craton. The studied samples exhibit similar textural and structural features but differ slightly in the mineral composition of the groundmass. They have a porphyritic structure, a massive texture, and consist of olivine macrocrystals embedded in a fully crystallized groundmass composed of perovskite, apatite, spinel, phlogopite, garnet, carbonates, clinopyroxene and other minerals. The macrocrystals quantity varies between 40 and 50 vol%. With the exception of a single sample from the Bushkanay dyke, olivine is entirely replaced by serpentine and/or talc.Perovskites from aillikites of the Bolshaya Tagna intrusion exhibit crystals with normal zoning showing a decrease in Na, REE, and Nb contents with center-to-rim increasing Ca content. In contrast, minerals from the aillikites of the Bushkanay dyke demonstrate reverse zoning, with an increase in Na, REE, and Sr and decrease in Ca contents from the center to the rim. We suggest that during crystallization of perovskites, the magma parental to the aillikites of the Bolshaya Tagna intrusion and the Bushkanay dyke had nearly similar trace element composition, but after crystallization of the cores of perovskite crystals each melt portion evolved independently. The samples from the Bushkanay dyke show an increase in fO2 and the residual magma enrichment in REE, Na, and Sr as evidenced by their elevated contents at the rims of perovskite grains. In the Bolshaya Tagna aillikites after crystallization of perovskite cores, the melt was depleted in REE, Na, and Nb.Based on U-Pb dating of perovskites, the age of aillikites from the Bolshaya Tagna intrusion is 583–654 Ma. Perovskite from aillikites of the Bushkanay dyke is relatively young, with an age of 575 ± 39 Ma. The obtained ages are consistent with the age of formation of Neoproterozoic alkaline-ultramafic carbonatite complexes of the Siberian craton and other occurrences of aillikites in a response to extension of the Rodinia lithosphere.

The impacts of pullulan soaking on radish seed germination and seedling growth under salt stress.

Pullulan can not only provide a source of organic carbon but also has excellent properties. However, current research is mostly limited to the physical properties of the high-molecular-weight components of pullulan, and little is known about the application of its low-molecular-weight components. This study was designed to explore the impact of presoaking of radish seeds in a pullulan solution on seed germination and subsequent seedling growth under salt stress conditions. Pullulan soaking was found to enhance the germination rates of radish seeds subjected to salt stress, while also enhancing the aboveground growth of radish seedlings. Pullulan soaking resulted in increases in chlorophyll, soluble protein, and soluble sugar concentrations in the leaves of these seedlings, together with greater peroxidase activity and root activity as well as decreases in Na+ and malondialdehyde concentrations. This provides an important reference for the application of pullulan in plant protection.

Foliar-applied silicate potassium modulates growth, phytochemical, and physiological traits in Cichorium intybus L. under salinity stress

One of the major problems endangering plant growth and productivity worldwide is salt stress. This study aimed to assess the effects of potassium silicate (K2O3Si) on the physical, biochemical, and morphological characteristics of chicory (Cichorium intybus L.) under various levels of salinity stress. The plants were treated with K2O3Si at concentrations of 0, 1, 2, and 3 mM and cultivated under different salt stress conditions (0, 80, 160, and 240 mM NaCl). The findings revealed that salt stress led to decreased root and shoot dry weights, Fv/Fm ratio, chlorophyll a, b, and total chlorophyll, as well as inulin contents. However, foliar exposure to K2O3Si at all salinity levels resulted in improvements in the measured traits. As salinity levels increased, there was a corresponding increase in the accumulation of sodium ions (Na+) and a sharp reduction in potassium ions (K +) in the shoot. Nonetheless, treatment with K2O3Si caused a decrease in Na + accumulation and an improvement in K+ content under all salinity levels. Carotenoid content increased under 80 mM salinity stress, but decreased with higher salinity levels. Application of K2O3Si at all levels resulted in increased carotenoid content under salinity stress conditions. The content of MDA increased significantly with increasing salinity stress, particularly at 240 mM. However, foliar spraying with K2O3Si significantly decreased MDA content at all salinity levels. Salinity stress up to 160 mM increased the total phenol, flavonoid, and anthocyanin contents, while 240 mM NaCl decreased the biosynthesis of phytochemicals. Additionally, the use of K2O3Si increased the content of total phenol, flavonoid, and anthocyanin at all salt levels. Foliar application of K2O3Si increased the tolerance of chicory plants to salinity stress by reducing MDA and increasing phenolic compounds and potassium content. These results suggest that exogenous K2O3Si can be a practical strategy to improve the growth and yield of chicory plants exposed to saline environments.

Effects of Heat Stress on the Laying Performance, Egg Quality, and Physiological Response of Laying Hens.

As high temperature and relative humidity (RH) are the main environmental factors causing heat stress, the temperature-humidity index (THI) serves as an indicator of heat stress in livestock animals. This study aimed to determine the effects of heat stress on the laying performance, physiological responses, egg quality, and blood profile of laying hens by subjecting them to environmental conditions with varying THI levels (68-85) for 28 days. The indicators of laying performance, such as feed intake (-30%) and egg production rate (-11%), significantly decreased in the hens exposed to severe heat stress (33 °C, 66% RH) compared to those exposed to thermoneutral conditions (21 °C, 68% RH). Moreover, severe heat stress reduced the egg yolk color, eggshell thickness and strength, and Haugh units of the eggs produced by the laying hens. Furthermore, a significant increase in serum K+ and a decrease in Na+ levels were observed in the hens subjected to severe heat stress compared with those under thermoneutral conditions. Our results indicate that heat stress alters the physiological responses and metabolism of laying hens, resulting in a lower egg quality and production rate.

Tannic acid ameliorates the hazards effect of beryllium induced neuro-alterations and oxidative stress in adult male rats.

Tannic acid (TA) is one of the most consumed and famous polyphenols with a widespread attention in the medical field according to its unique structural, pharmaceutical, physicochemical, antioxidant and other biological features. A rare study was conducted on the hazard effect of beryllium (Be) on the central nervous system. This study aims to show the ability of beryllium to cross the blood brain barrier. Demonstrate the effect of beryllium and tannic acid separately or with each other on brain ions (Na+, K+, Ca++) and on norepinephrine, dopamine, serotonin, finally on the glutathione and malondialdehyde. Seventy-two rats were divided into four groups as control, Be, TA, and Be+TA where Be was injected intraperitoneally as 1mg/Kg b. wt, TA was orally administrated as 5% in aquas solution. The administration of beryllium showed its ability to cross the blood brain barrier and accumulated in cortex > cerebellum>hypothalamus also, a significant increase in Na+, Ca++ cooperated with a significant decrease in K+ ions content was observed. Norepinephrine, dopamine, and serotonin showed a general significant decrease in their content joined with a significant decrease in glutathione (GSH) and elevation in malondialdehydes (MDA) because of Be intoxication. On the other hands the daily oral administration of tannic acid showed a general significant decrease in Na+, Ca++ ions content parallel with a significant increase K+ also, a non-significant change in the three measured neurotransmitters was noticed. Tannic acid showed a mitigation effect against Be intoxication which may regarded to the tannic acid antioxidant, chelating effect.

ATP-dependent ion-transport systems functioning in immunocompetent cells of men with erectile dysfunction due to combat trauma

Background. The study of the problem of physical and mental health of people who have survived combat trauma is a component of a wide field of research on the problem of stress, which manifests itself at all levels of the organization of the body. Combat injuries, in particular those of the areas of pelvis and genitourinary system, stressful events, lead to the development of erectile dysfunction (ED). In recent decades, a sufficient number of scientific facts have been accumulated, which confirm the significant influence of stress factors on the reduction of sexual desire and sexual activity. Therefore, determination of Ca2+,Mg2+- and Na+,K+-ATPase activity on a convenient model like peripheral blood lymphocytes add complexity to the understanding of the development of the pathophysiological and pathobiochemical mechanisms of the body, the result of which is the development of ED. Materials and methods. The research was conducted on peripheral blood lymphocytes of men injured as a result of combat operations (shrapnel and bullet wounds) in the Russian-Ukrainian war and treated at the Military Medical Clinical Center of the Western Region. The research group of men with combat injuries was divided into two subgroups: men aged 20–39 years (subgroup 1) and men aged 40–53 years (subgroup 2). The control group consisted of 48 practically healthy men without complaints of sexual dysfunction or cardiac, neurological or endocrinological pathology. Among the men of the control group were 30 men aged 20–39 years (subgroup 3) and 18 men aged 40–53 years (subgroup 4). Results. It has been shown that in the peripheral blood lymphocytes of men injured as a result of hostilities, there is a decrease in Na+,K+-ATPase activity and Ca2+,Mg2+-ATPase activity of the plasma membrane and endoplasmic reticulum, which leads to overloading of the cytosol with Na+ and Ca2+ ions, respectively, which is characteristic of pathological processes. Conclusion. Erectile dysfunction due to combat trauma is accompanied by a decrease in both Ca2+,Mg2+-ATPase activity of the plasma membrane and Ca2+,Mg2+-ATPase activity of endoplasmic reticulum of blood lymphocytes. As the age of patients with disorders of sexual function increases, the decrease in Ca2+,Mg2+-ATPase activities becomes more expressed. In men with erectile dysfunction due to combat trauma, the activity of Na+,K+-ATPase is also inhibited. According to the ROC curve, Ca2+,Mg2+-ATPase activity of the endoplasmic reticulum in blood lymphocytes is a potential biomarker of erectile dysfunction.

Mechanisms of oxidative response during biodegradation of malathion by S. oneidensis MR-1

Malathion, an extensively used organophosphorus pesticide, poses a high potential risk of toxicity to humans and the environment. Shewanella (S.) oneidensis MR-1 has been proposed as a strain with excellent bioremediation capabilities, capable of efficiently removing a wide range of hard-to-degrade pollutants. However, the physiological and biochemical response of S. oneidensis MR-1 to malathion is unknown. Therefore, this study aimed to examine how S. oneidensis MR-1 responds physiologically and biochemically to malathion while also investigating the biodegradation properties of the pesticide. The results showed that the 7-day degradation rates of S. oneidensis MR-1 were 84.1, 91.6, and 94.0% at malathion concentrations of 10, 20, and 30 mg/L, respectively. As the concentration of malathion increased, superoxide dismutase and catalase activities were inhibited, leading to a significant rise in malondialdehyde content. This outcome can be attributed to the excessive production of reactive oxygen species (ROS) triggered by malathion stress. In addition, ROS production stimulates the secretion of soluble polysaccharides, which alleviates oxidative stress caused by malathion. Malathion-induced oxidative damage further exacerbated the changes in the cellular properties of S. oneidensis MR-1. During the initial stages of degradation, the cell density and total intracellular protein increased significantly with increasing malathion exposure. This can be attributed to the remarkable resistance of S. oneidensis MR-1 to malathion. Based on scanning electron microscopy observations, continuous exposure to contaminants led to a reduction in biomass and protein content, resulting in reduced cell activity and ultimately leading to cell rupture. In addition, this was accompanied by a decrease in Na+/K+- ATPase and Ca2+/Mg2+-ATPase levels, suggesting that malathion-mediated oxidative stress interfered with energy metabolism in S. oneidensis MR-1. The findings of this study provide new insights into the environmental risks associated with organophosphorus pesticides, specifically malathion, and their potential for bioremediation.Graphical

Physiological responses of oxidative damage, genotoxicity and hematological parameters of the toxic effect of neonicotinoid-thiamethoxam in Oreochromis niloticus

The purpose of investigation assessed the impacts of neonicotinoid thiamethoxam (TMX) at sublethal concentrations in hematological profile and renal function of Oreochromis niloticus. In the experiment, fish were exposed to TMX in four groups (0, 50, 100 and 150 ppm) for 7 days. At the end of the experiment, biochemical analysis of blood samples showed that the parameters indicating renal function showed a significant increase in serum enzymes ALT, AST, ALP and metabolites (BUN, urea, uric acid, creatinine and cortisol) concentrations, while albumin concentration decreased in a dose-dependent manner compared to the control group. In parallel with the decrease in Na+, K+ and Ca+2 in blood ion levels, there was a significant decrease in the activity of Na+/K+ ATPase, Ca+2 ATPase and AChE enzyme, levels of GSH and HSP70 in kidney tissue in TMX groups compared to the control group. It was determined that the toxic effect of TMX caused a significant increase in TBARS, PC, 8-OHdG levels, respectively. In conclusion, our study shows that TMX causes dose-dependent toxic effects, with knock-on effects on physiological processes regarding the hematological profile and renal function of O. niloticus.

Pemetrexed alleviates piglet diarrhea by blocking the interaction between porcine epidemic diarrhea virus nucleocapsid protein and Ezrin.

Porcine epidemic diarrhea virus (PEDV) is an enteric coronavirus that causes high mortality in piglets, thus posing a serious threat to the world pig industry. Porcine epidemic diarrhea (PED) is related to the imbalance of sodium absorption by small intestinal epithelial cells; however, the etiology of sodium imbalanced diarrhea caused by PEDV remains unclear. Herein, we first proved that PEDV can cause a significant decrease in Na+/H+ exchanger 3 (NHE3) expression on the cell membrane, in a viral dose-dependent manner. Further study showed that the PEDV nucleocapsid (N) protein participates in the regulation of NHE3 activity through interacting with Ezrin. Flame atomic absorption spectroscopy results indicated a serious imbalance in Na+ concentration inside and outside cells following overexpression of PEDV N. Meanwhile, molecular docking technology identified that the small molecule drug Pemetrexed acts on the PEDV N-Ezrin interaction region. It was confirmed that Pemetrexed can alleviate the imbalanced Na+ concentration in IPEC-J2 cells and the diarrhea symptoms of Rongchang pigs caused by PEDV infection. Overall, our data suggest that the interaction between PEDV N and Ezrin reduces the level of phosphorylated Ezrin, resulting in a decrease in the amount of NHE3 protein on the cell membrane. This leads to an imbalance of intracellular and extracellular Na+, which causes diarrhea symptoms in piglets. Pemetrexed is effective in relieving diarrhea caused by PEDV. Our results provide a reference to screen for anti-PEDV targets and to develop drugs to prevent PED.IMPORTANCEPorcine epidemic diarrhea (PED) has caused significant economic losses to the pig industry since its initial outbreak, and the pathogenic mechanism of porcine epidemic diarrhea virus (PEDV) is still under investigation. Herein, we found that the PEDV nucleocapsid protein interacts with Ezrin to regulate Na+/H+ exchanger 3 activity. In addition, we screened out Pemetrexed, a small molecule drug, which can effectively alleviate pig diarrhea caused by PEDV. These results provide support for further exploration of the pathogenesis of PEDV and the development of drugs to prevent PED.

Effect of replacing B2O3 with Dy2O3 on the structural, physical, and radiation shielding properties of sodium boroaluminate glass

AbstractIn this work, some transparent aluminum sodium borate-based glasses containing Dy2O3 have been prepared using the melt quenching method. The incorporation of Dy3+ ions in the glass network leads to increase and decrease the density and molar volume, respectively. The structural properties are investigated by XRD and FTIR spectroscopy. It is revealed that addition of Dy2O3 causes a conversion of boron coordination from BO4 to BO3, which indicates the increase in the number of non-bridging oxygen. Therefore, the optical band gap is found to decrease as the Dy3+ ions content increases. The temperature dependences of dielectric constant and AC conductivity are studied at different frequencies. The electric conductivity and dielectric parameters decrease with increasing Dy3+ content due to the decrease in Na+ ions mobility due to the blocking effect of Dy3+ cations in the glass network. The shielding factors have been evaluated for the prepared glasses with the help of Phy-X program. The maximum linear attenuation coefficient (LAC) is found at 0.284 MeV and varied between 0.125 and 0.140 cm−1. The results revealed that the incorporation of Dy2O3 into the glasses has a substantial effect on the Zeff.. The value of the Zeff for the D1 sample, which does not include any Dy2O3, stays relatively the same, ranging around 7.51. We found that the rate of reduction in Zeff was significantly high when the energy of the photons is smaller than 0.826 MeV. From the Zeff data, we found that the addition of Dy2O3 to the glasses improves both their capacity to absorb and their capability to scatter ionizing radiation.

Herbal medicine (Oryeongsan) for fluid and sodium balance in renal cortex of spontaneously hypertensive rats

BackgroundHerbal medicine Oryeongsan (ORS), also known as Wulingsan in Chinese, has been used for the treatment of impaired body fluid balance. However, the mechanisms involved are not clearly defined. The purpose of the present study was to identify the actions of ORS on the renal excretory function and blood pressure (BP) and to define the mechanisms involved in association with renin-angiotensin system (RAS) and natriuretic peptide system (NPS) in spontaneously hypertensive rats (SHR), an animal model of human essential hypertension. MethodsChanges in urine volume (UV), excretion of electrolytes including Na+ (urinary excretion of Na+ (UNaV)) were measured. RT-PCR was performed to trace the changes in expression of RAS, NPS and sodium (Na+)-hydrogen (H+) exchanger 3 (NHE3) in the renal cortex. ResultsIn the SHR treated with vehicle (SHR-V) group, UV and UNaV were suppressed and the Na+ balance was maintained at the higher levels leading to an increase in BP compared to WKY-V group. These were accompanied by an increase in NHE3 expression with an accentuation of angiotensin I converting enzyme-angiotensin II type 1 (ACE-AT1) receptor and concurrent suppression of angiotensin II type 2 (AT2) receptor/ACE2-Mas receptor expression in the renal cortex. Chronic treatment with ORS increased UV and UNaV, and decreased the Na+ and water balance with a decrease in BP in the ORS-treated SHR-ORS group compared to SHR-V. These were accompanied by a decrease in NHE3 expression with a suppression of ACE-AT1 receptor and concurrent accentuation of AT2/ACE2-Mas receptor. ConclusionThe present study shows that ORS reduced BP with a decrease in Na+ and water retention by a suppression of NHE3 expression via modulation of RAS and NPS in SHR. The present study provides pharmacological rationale for the treatment of hypertension with ORS in SHR.

A novel PGPR strain homologous to Beijerinckia fluminensis induces biochemical and molecular changes involved in Arabidopsis thaliana salt tolerance

The use of PGPR is widely accepted as a promising tool for a more sustainable agricultural production and improved plant abiotic stress resistance. This study tested the ability of PVr_9, a novel bacterial strain, homologous to Beijerinckia fluminensis, to increase salt stress tolerance in A. thaliana. In vitro plantlets inoculated with PVr_9 and treated with 150 mM NaCl showed a reduction in primary root growth inhibition compared to uninoculated ones, and a leaf area significantly less affected by salt. Furthermore, salt-stressed PVr_9-inoculated plants had low ROS and 8-oxo-dG, osmolytes, and ABA content along with a modulation in antioxidant enzymatic activities. A significant decrease in Na+ in the leaves and a corresponding increase in the roots were also observed in salt-stressed inoculated plants. SOS1, NHX1 genes involved in plant salt tolerance, were up-regulated in PVr_9-inoculated plants, while different MYB genes involved in salt stress signal response were down-regulated in both roots and shoots. Thus, PVr_9 was able to increase salt tolerance in A. thaliana, thereby suggesting a role in ion homeostasis by reducing salt stress rather than inhibiting total Na+ uptake. These results showed a possible molecular mechanism of crosstalk between PVr_9 and plant roots to enhance salt tolerance, and highlighted this bacterium as a promising PGPR for field applications on agronomical crops.

Characteristics, geochronology, and formation conditions of W mineralization and its relationship with Au mineralization in the Zhaishang gold deposit, West Qinling Orogen, China

The West Qinling Orogen is an important Au-W ore belt; however, the spatio-temporal and genetic relationships of W and Au ores remain unclear. This study takes the Zhaishang deposit, one of the largest Au-W deposits in the West Qinling Orogen, to trace their connections. The geological observation reveals that high-grade W ores are commonly characterized by extensional hydrothermal veins. In contrast, high-grade Au ores are hosted by breccia zones, which cut the W ore-controlling structures, even though the W and Au ores overlap locally. The mineral assemblages of W ores associated with carbonatization and silicification consist of scheelite and pyrite with few polymetallic sulfides, in contrast to the abundance of polymetallic sulfides and native gold in Au ores linked with silicification. Textural and in situ trace element analyses reveal that the scheelites show a decrease in Na, U, Mo, Nd, and calculated Eu anomaly values and an increase of Th concentrations and Ce anomaly values from early- to late-generation scheelites. This finding, in conjunction with mineral assemblages and previous thermodynamic modeling, suggests a decrease in salinity and oxygen fugacity (fO2) of the oxidized ore-forming fluids of W mineralization, as opposed to the reduced fluids of Au mineralization. The plots of scheelite at Zhaishang in the ternary LREE-MREE-HREE diagram are compatible with those of W deposits related to magmatic-hydrothermal fluids. In situ U-Pb dating of scheelite from W ores constrains the precise timing of W mineralization at ca. 227.9 ± 2.3 and 225.6 ± 3.0 Ma in a collisional orogenic setting. The unequivocal crosscutting relationship, contrasting mineral assemblages, and physiochemical conditions suggest that the W and Au mineralization at Zhaishang are due to two separate mineralization events. The identification of overlapped W and Au mineralization events at Zhaishang is crucial for guiding the regional exploration of W and Au deposits.

Micro-meso-macroscale correlation mechanism of red-bed soft rocks failure within static water based on energy analysis

The purpose of the present study is to fundamentally investigate the micro-meso-macroscale correlation mechanism of red-bed soft rocks failure within static water, based on energy analysis. Through a series of experimental studies, changes in the micro-meso-macroscale characteristics of the rocks were characterised by measuring ion concentrations in and pH of water, the pore structure and crack propagation. Based on the experimental measurements, theoretical modelling of micro-meso-macroscale energy conservation was carried out to determine the micro-meso-macroscale correlation mechanism of red-bed soft rocks failure. The results show that the microscale dominant effect of red-bed soft rocks is determined as chemical effects. The failure mechanism of red-bed soft rocks under static water saturation involves chemical, physical and mechanical dominant effects at the micro-meso-macroscale. It was demonstrated that the dominant chemical effect of clay minerals and water comprises a series of chemical energy changes in the microscale water–rock interface. This can induce the energy changes in both mesophysical and macromechanical levels. During the first month after the water saturation, there was a significant decrease in Na+ and K+ concentrations in aqueous solution, whereas there was little change in Ca+ and Mg2+ concentrations. The scanning electron microscope (SEM) images indicated an obvious change in microstructure of the red-bed soft rocks after 3 months of water saturation. A decrease in pore number and increase in total pore surface area could be seen after 6 months of water saturation. Furthermore, the theoretical results suggest that chemical effects at the microscale dominate the softening process of the rocks. During the water–rock interaction, nearly 80% of the energy dissipation results from the microscale change, and the meso-macroscale change accounts for the remaining 20%. As a uniform measure of material evolution, energy can correlate the micro-meso-macro dominant effect of red-bed soft rocks softening appropriately.

Volatile cinnamaldehyde induces systemic salt tolerance in the roots of rice (Oryza sativa).

Cinnamaldehyde (CA) is a volatile plant secondary metabolite that exhibits strong anti-pathogenic activities. Nonetheless, less is known about the effect of CA on plant tolerance to abiotic stresses. In this study, we delineated the effects of CA fumigation on rice roots (Oryza Sativa L cv. TNG67) under salinity stress (200 mM NaCl). Our result showed that CA vapor significantly alleviated salinity-induced ROS accumulation and cell death. This CA-induced alleviation appears to be mediated primarily by the upregulation of proline metabolism genes, the rapid proline accumulation, and the decrease of Na+ /K+ ratio as early as 3 h after NaCl treatment. Of note, the activities of peroxidase (POD; EC 1.11.1.7) isozymes a and b were decreased by CA fumigation, and the activities of catalase (CAT; EC 1.11.1.6) and superoxide dismutase (SOD; EC 1.15.1.1) were not significantly affected. Our findings suggest that CA vapor might be useful for priming rice roots to withstand salinity stress, which is more prevalent due to the ongoing global climate change. To the best of our knowledge, this is the first study to show modulation of macro- and micro-elements as well as antioxidative factors after CA fumigation of salinity-stressed rice roots.

Organic Amendments Improved the Productivity and Bio-Fortification of Fine Rice by Improving Physiological Responses and Nutrient Homeostasis under Salinity Stress.

Salinity stress (SS) is major abiotic stress that is seriously limiting crop production across the globe. The application of organic amendments (OA) mitigate the effects of salinity and improves soil health and crop production on a sustainable basis. However, limited studies are conducted to determine the impact of farmyard manure (FYM) and press mud (PM) on the performance of rice crop. Therefore, we performed this study to determine the impacts of FYM and PM on the growth, physiological and biochemical attributes, yield, and grain bio-fortification of rice crop under SS. The experiment was comprised of SS levels; control, 6 and 12 dS m-1 SS and OA; control, FYM: 5%, press mud 5% and combination of FYM (5%) + PM (5%). Soil salinity imposed deleterious impacts on the growth, yield, and grain quality of rice, however, OA appreciably offset the deleterious impacts of SS and improved the growth, yield, and grain bio-fortification of rice crop. The combined application of FYM + PM improved the growth and yield of rice through an increase in chlorophyll contents, leaf water contents, anti-oxidant activities (ascorbate peroxidise: APX; catalase: CAT, peroxidise: POD and ascorbic acid: AsA), K+ accumulation and decrease in Na+/K+ ratio, electrolyte leakage, malondialdehyde (MDA), hydrogen peroxide (H2O2), Na+ accumulation. Moreover, the combined application of FYM + PM significantly improved the grain protein (5.84% and 12.90%), grain iron (40.95% and 42.37%), and grain zinc contents (36.81% and 50.93%) at 6 and 12 dS m-1 SS. Therefore, this study suggested that the application of FYM and PM augmented the growth, yield, physiology, biochemistry, and grain bio-fortification of rice and proved to be a good practice for better rice production in salt-affected soils.

Comparative Effects of Phyllantus Amarus on the Serum Electrolyte Level of Hypertensive and Normotensive Male Wistar Rats

Highlights: It discusses effects of phyllantus amarus on serum electrolyte which results in an imbalance. The reducing effect oh sodium and chloride levels implicate a new chance in managing hypertension. Abstract: Background. High blood pressure, also known as hypertension, is one of the most well-known major risk factors for cardiovascular disease (CVD) and stroke. Aims. This present study tried to compare the effect of P.amarus leaves on normotensive and hypertensive male Wistar rats. Materials and method. 15 male Wistar rats were divided into three groups of five rats each (Control group, Control + Extract group, and Hypertensive+ Extract group). One of the groups of rats (group 3) was induced with hypertension using 0.5mg/kg for 5 days of Dexamethasone. Then, the normal/control group and the hypertensive were treated with 200mg/kg of aqueous Extract of Phyllantus amarus (AEPA) for 28 days. Fasting venous blood samples were collected for the measurements of serum electrolytes. Serum potassium, sodium, and chlorine levels were measured by the ion-selective electrode method. Results. In this experiment, for the normotensive and hypertensive treatment groups respectively, there was an increase in K⁺ level and decrease in K⁺ level compared to the control rats, decrease in Na⁺ level, and decrease in Na⁺ level. Also, a decrease in Clˉ of normotensive rats while there was no change in the Clˉ level of hypertensive group when compared to control group. The p values for all results were found to be non-significant. Phyllantus amarus has an effect on serum electrolyte imbalance. Its reducing effect on sodium and chloride levels implies that it can be used to manage hypertension, thereby reducing the risk of developing cardiovascular diseases.