You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Peyronie's Disease1 Apr 2015PD48-04 EFFECTS OF COLLAGENASE TREATMENT ON BIOMECHANICAL PROPERTIES OF PEYRONIE'S PLAQUES Thomas Schmid, Vincent Wang, Elizabeth Shewman, Michael McLane, James Tursi, and Laurence Levine Thomas SchmidThomas Schmid More articles by this author , Vincent WangVincent Wang More articles by this author , Elizabeth ShewmanElizabeth Shewman More articles by this author , Michael McLaneMichael McLane More articles by this author , James TursiJames Tursi More articles by this author , and Laurence LevineLaurence Levine More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.2761AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The purpose of this study was to measure the biomechanical properties of tissues recovered from surgical correction of patients with Peyronie's Disease (PD) and determine if these properties change after enzymatic treatment with a purified bacterial collagenase (AA4500). METHODS Tissue specimens were collected from PD patients after surgery with patients' consent and institutional approval. Specimens were stored frozen in saline. Strips of tunica albuginea from PD plaques were isolated from two orthogonal directions, parallel to (longitudinal strips), and perpendicular to (circumferential strips) the long axis of the penis, and used for tensile testing. Tensile tests were performed on 2 × 12 mm tissue strips. Specimens with mineral foci were detected by x-ray, and excluded from the study. RESULTS The data revealed substantial variation in the biomechanical parameters of different donor tissues. Less variation was evident in adjacent tissue strips taken from the same orientation (longitudinal or circumferential) compared to strips from different PD donors. The circumferential strips supported a higher maximal load and work to maximum load than similar sized longitudinal strips. The strain (amount tissue stretched) was greater for longitudinal than circumferential sections. Additional tissues were injected with clinical concentrations of collagenase AA4500. Strips treated with the collagenase showed a dramatic decrease in maximum load, stiffness, work to maximum load, and maximum stress (see figure). CONCLUSIONS Large variations in tensile properties across multiple PD donor tissues could be minimized by comparing adjacent longitudinal versus circumferential strips from single donors. The injection of collagenase AA4500 into PD plaque tissues caused a dramatic reduction in tensile properties. These experiments are a proof-of-concept that treatment of PD plaques with collagenase in vitro reduces tissue biomechanical properties. Such in vitro testing may help optimize injection protocols for the therapeutic use of AA4500 collagenase in PD patients. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e967 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Thomas Schmid More articles by this author Vincent Wang More articles by this author Elizabeth Shewman More articles by this author Michael McLane More articles by this author James Tursi More articles by this author Laurence Levine More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...
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