Days Of Experimental Infection Research Articles

Ameliorative effects of propolis and wheat germ oil on acute toxoplasmosis in experimentally infected mice are associated with reduction in parasite burden and restoration of histopathological changes in the brain, uterus, and kidney.

Toxoplasmosis continues to be a prevalent parasitic zoonosis with a global distribution. This disease is caused by an intracellular parasite known as Toxoplasma gondii, and the development of effective novel drug targets to combat it is imperative. There is limited information available on the potential advantages of wheat germ oil (WGO) and propolis, both individually and in combination, against the acute phase of toxoplasmosis. In this study, acute toxoplasmosis was induced in Swiss albino mice, followed by the treatment of infected animals with WGO and propolis, either separately or in combination. After 10 days of experimental infection and treatment, mice from all groups were sacrificed, and their brains, uteri, and kidneys were excised for histopathological assessment. Additionally, the average parasite load in the brain was determined through parasitological assessment, and quantification of the parasite was performed using Real-Time Polymerase Chain Reaction targeting gene amplification. Remarkably, the study found that treating infected animals with wheat germ oil and propolis significantly reduced the parasite load compared to the control group that was infected but not treated. Moreover, the group treated with a combination of wheat germ oil and propolis exhibited a markedly greater reduction in parasitic load compared to the other groups. Similarly, the combination treatment effectively restored the histopathological changes observed in the brain, uterus, and kidney, and the scoring of these reported lesions confirmed these findings. In summary, the present results reveal intriguing insights into the potential therapeutic benefits of wheat germ oil and propolis in the treatment of acute toxoplasmosis.

Effect of Aflatoxin B1 on farmed Cyprinus carpio in conjunction with bacterial infection

Aflatoxin B1 (AFB1) is an immunosuppressive plant-origin toxin that reduces disease resistance of fish. A total number of 360 fries of the common carp, Cyprinus carpio(0.50 g/fish b.w.) were subdivided into 6 groups (G1-G6). Fish were fed on an AFB1-contaminated diet at a dose of 0.5 and 1.0 mg/kg fish feed in G2 and G3, respectively. AFB1-contaminated diet plus nucleotides (NT) supplementation (at 0.2% instead of normal corn) were G5 and G6, respectively. Fish in G1 were fed on a diet free from AFB1 and NT while in G4, fish were fed on a diet free from AFB1 and supplemented with NT. Growth performance and liver enzymes (ALT, AST, and ALP) were adversely impacted by feeding on a contaminated diet and NT supplementation could ameliorate such withdraws. Anti-inflammatory IL-10 was increased in groups fed on contaminated diet indicating that inflammation had taken place. Feeding on AFB1-contaminated diets resulted in degenerative changes (hepatic and spleen tissues), NT-supplementation could relieve such changes (low dose of AFB1) however impacts of the high dose of AFB1 were severe to a degree that NT-supplementation could not effectively diminish it. Regardless of feeding on the contaminated diet, C. carpio supplemented with NT could resist Aeromonas hydrophila infection even those fed on contaminated diets, while un-supplemented groups showed higher mortality rates. A. hydrophila could isolate even after 35 days of experimental infection in C. carpio fed on AFB1-contaminated diet (G2 and G3). It concluded that NT supplementation could ameliorate the adverse health impacts associated with feeding diet contaminated with AFB1 in C. carpio.

Serological diagnosis of equine infectious anemia in horses, donkeys and mules using an ELISA with a gp45 synthetic peptide as antigen

Equine infectious anemia (EIA) is a disease caused by a Lentivirus that is currently controlled exclusively by identification of seropositive animals. In most countries, including Brazil, the official diagnostic test for EIA is the agar gel immunodiffusion test (AGID). Although this assay has a high specificity it can produce false negative reactions or equivocal results due to weak precipitation lines, especially in samples from donkeys, mules or newly infected equids. In this pioneering study, it was used overlapping synthetic peptide pools to map and identify a consensus, widely recognised antibody epitope within env encoding the EIAV envelope proteins. A 20-mer soluble peptide encompassing this epitope (pgp45) was then synthesized and tested in an indirect ELISA test. Using a panel of 859 EIA positive and negative equid serum samples, the pgp45 ELISA had 96.1% concordance, 98.6% sensitivity and 95.6% specificity respectively, when compared to AGID. The sensitivity and specificity of the pgp45 ELISA was also >90% when tested in individual equid species including horses (Equus caballus), donkeys (Equus asinus) and mules (Equus caballus x Equus asinus). Moreover, in a horse experimentally infected with the pathogenic Wyoming EIAV strain viral-specific antibodies were detected at 10 days post-infection (dpi) whereas in AGID no specific antibody was detected until 18 days of experimental infection. This peptide can now be used as an antigen in serological tests, especially for rapid screening of large numbers of equids, where it may contribute significantly in the control of EIA, especially at sites with high populations of donkeys and mules.

Bartonella henselae transmission by blood transfusion in mice.

Bartonella spp. are neglected fastidious Gram-negative bacilli. We isolated Bartonella henselae from 1.2% of 500 studied blood donors and demonstrated that the bacteria remain viable in red blood cell units after 35 days of experimental infection. Now, we aim to evaluate the possibility of B. henselae transmission by blood transfusion in a mouse model. Eight BALB/c mice were intraperitoneal inoculated with a 30 µL of suspension with 10(4) CFU/mL of B. henselae and a second group of eight mice were inoculated with saline solution and used as control. After 96 hours of inoculation, the animals were euthanized. We collected blood and tissue samples from skin, liver, and spleen. Thirty microliters of blood from four Bartonella-inoculated animals were transfused into a new group (n = 4). Another group received blood from the control animals. B. henselae infection was investigated by conventional and nested polymerase chain reaction (PCR). Blood samples from all 24 mice were negative by molecular tests though half of the tissue samples were positive by nested PCR in the intraperitoneal Bartonella-investigated animals. Tissues from two of the four mice that received blood transfusions from Bartonella-inoculated animals were also nested PCR positives. Transmission of B. henselae by transfusion is possible in mice even when donor animals have undetectable bloodstream infection. The impact of human Bartonella sp. transmission through blood transfusion recipients must be evaluated.

Early and late B cell immune responses in lethal and self-cured rodent malaria

ICR mice have heterogeneous susceptibility to lethal Plasmodium yoelii yoelii 17XL from the first days of experimental infection as evidenced by the different parasitemia levels and clinical outcomes. This mouse model has revealed specific immune responses on peripheral blood correlating with the infection fate of the animals. To search for immune-markers linked to parasitemia we examined B lymphocytes in organs of the immune system as key effectors of rodent immunity against malaria. To determine changes in immune cellularity fostered by the different prognostic parasitemia we examined B cell subsets in low (<15%) and high (>50%) parasitized mice during the first days of the infection. In the case of surviving mice, we studied the preservation of memory immune response 500 days after the primary P. yoelii challenge. Correlating with the parasitemia level, it was observed an increase in total cellularity of spleen during the first week of infection which remained after 16 months of the infection in surviving animals. B cell subsets were also modified across the different infection fates. Subpopulation as follicular B cells and B-1 cells proportions behaved differently depending on the parasitemia kinetics. In addition, peritoneal cavity cells proliferated in response to high parasitemia. More significantly, P. yoelii -specific memory B cells remained in the spleen 500 days after the primo-infection. This study demonstrates that B cell kinetics is influenced by the different parasitemia courses which are naturally developed within a same strain of untreated mice. We show that high levels of parasitemia at the beginning of infection promote an extremely fast and exacerbate response of several cell populations in spleen and peritoneal cavity that, in addition, do not follow the kinetics observed in peripheral blood. Furthermore, our results describe the longest persistence of memory B cells long time upon a single malaria infection in mice.

Detection of Trichinella spiralis DNA in mouse faeces during the early stage of infection

Trichinella spiralis is an etiological agent of trichinellosis, a parasitic disease present worldwide, affecting both animals and humans. The infection starts from the intestinal stage which usually lasts a few weeks and develops into the muscle phase when larvae enter the muscle cells and create a nurse cell-larva-complex. In animals, the infection is asymptomatic even if a high number of larvae is ingested. The aim of this work was to develop a sensitive test to monitor the early phase of trichinellosis in laboratory animals. T. spiralis DNA presence was assayed in faeces of mice during the first 21 days of experimental infection. The target gene fragment of the mitochondrial ATP6 synthase F0 subunit was amplified by PCR from faecal samples of mice infected with 50, 250 or 500 larvae per mouse (lpm). Trichinella DNA was detected in 45% (116) of the examined faecal samples from days 1 to 21 post infection (p.i.). The time of appearance of the parasite DNA in the faeces and the percentage of PCR positive results were dose dependent. In collective samples consisting of faeces from 3 mice average 80%, 83% and 90% of PCR positive results were obtained for 50, 250 and 500 lpm doses, respectively, throughout 21 days of the observation period. In individual mice faeces DNA was detected on days 2–19 and 9–18 p.i. for 250 lpm and 50 lpm doses, respectively. For both infective doses the highest probability of DNA detection was on day 11 p.i. Since the developed test is simple and non-invasive, it can be useful for monitoring the intestinal phase of Trichinella sp. infection in laboratory animals.

Giardiosis in camels (Camelus dromedarius)

Giardiosis in camels (<i>Camelus dromedarius</i>)

Early hepatic cytokine mRNA expression in experimental rat fasciolosis

We studied the development of the cellular response, particularly with respect to Th1 and Th2 cytokine mRNA levels, in rat liver during the first 14 days of experimental infection with Fasciola hepatica. We analysed the panel of cytokines involved in initiation of the inflammatory and immune response. The levels of various mRNAs, particularly those primarily associated with the acute inflammatory response, and those commonly associated with T-cell proliferation and differentiation, were assessed by reverse transcription-polymerase chain reaction (RT-PCR) in liver samples. We also investigated the immune and inflammatory mediators balance in the liver, draining lymph node and spleen, by RT-competitive PCR quantification of mRNA levels for IL-4, IL-10 and IFN-γ. Our data provide the first evidence that, in the early phase of infection, the inflammatory response in the liver of infected animals is transiently depressed or delayed. A Th0 profile was initially observed in the liver and hepatic lymph node, which developed into a Th2 profile 2 weeks after infection in the liver only. In the spleen, cytokine down-regulation was initiated and maintained during this period, suggesting that the parasite acts differently locally and in the periphery.

STP and Tip are essential for herpesvirus saimiri oncogenicity.

Mutant forms of herpesvirus saimiri (HVS) subgroup C strain 488 with deletions in either STP-C488 or Tip were constructed. The transforming potentials of the HVS mutants were tested in cell culture and in common marmosets. Parental HVS subgroup C strain 488 immortalized common marmoset T lymphocytes in vitro to interleukin-2-independent growth, but neither of the deletion mutants produced such growth transformation. Wild-type HVS produced fatal lymphoma within 19 to 20 days of experimental infection of common marmosets, while HVS deltaSTP-C488 and HVS deltaTip were nononcogenic. Virus was repeatedly isolated from the peripheral blood of marmosets infected with mutant virus for more than 5 months. These results demonstrate that STP-C488 and Tip are not required for replication or persistence, but each is essential for transformation in cell culture and for lymphoma induction in common marmosets.

Dynamics of the interaction between the parasitic isopod, Anilocra pomacentri, and the coral reef fish, Chromis nitida

Field and laboratory investigations into the effect of the parasitic isopod Anilocra pomacentri (Cymothoidae) on the population dynamics of the reef fish Chromis nitida (Pomacentridae) were carried out at Heron Island, Great Barrier Reef, Australia. Fish carried a single adult parasite just posterior and dorsal to the eye either to the right or to the left of the midline. The adult parasite was overdispersed among fish on patch reefs (dispersion factor, k = 0.69). Sequential field observations on a single cohort of fish showed that parasites significantly depressed growth, reproduction, and survivorship. The von Bertalanffy growth coefficients (a measure of somatic growth) were 0.10 for parasitized fish compared with 0.17 for non-parasitized fish. Female fish carrying the parasite produced only 12% of the number of eggs produced by non-parasitized fish of the same size. In the field, the mortality of infected juvenile C. nitida (LCF 15-30 mm) was estimated to be at least 88% in the first 70 days after recruitment of the fish. The mortality of uninfected recruits over the same period was 66%. In laboratory trials, the mortality associated with the infection of juvenile fish by larval parasites ranged from 78% for small fish (mean LCF 15.0 mm) to 28% for larger fish (mean LCF 24.9 mm) within 4 days of experimental infection. This is one of the few studies that evaluates the effect of a parasite on a population of fish in the field.