AbstractHemolymph proteins of the Douglas-fir cone moth,Barbara colfaxiana(Kft.), in the year of pupation (year P) and the next year (year P + 1) were studied by visual examination and analysis of digitized images of isoelectric focussing patterns. Differences in these proteins between light and dark individuals in year P and P + 1 were not related to dormancy duration but reflected the extent of pharate-adult development. Hormone applications were more effective in changing hemolymph proteins of light insects than those of dark ones. Changes in the hemolymph proteins of light insects in year P + 1 were not very obvious by visual examination of gels but were readily detectable by digital image analysis and showed that development occurred in these insects through June-September leading to visible pigmentation differences observable in relation to dormancy commitments of these insects.