Dark Markings Research Articles

Two new hypogean species of the genus Triplophysa (Osteichthyes, Cypriniformes, Nemacheilidae) from Guizhou Province, Southwest China, with underestimated diversity.

Two new species of the genus Triplophysa from southwestern Guizhou Province, China, are described. These are Triplophysaziyunensis Wu, Luo, Xiao & Zhou, sp. nov. and Triplophysayaluwang Lan, Liu, Zhou & Zhou, sp. nov. from Maoying Town, Ziyun County, Guizhou Province, China. Triplophysaziyunensis Wu, Luo, Xiao & Zhou, sp. nov. is distinguished from other hypogean species of the genus Triplophysa by having a combination of the following characteristics: body naked, scaleless, pigmented markings on surface of body, except ventral; eyes reduced, diameter 2.4-4.9% of head length; pelvic-fin tip extending to anus; tip of pectoral fin not reaching pelvic fin origin; anterior and posterior nostrils closely set, with anterior nostril elongated to a barbel-like tip; tip of outrostral barbel extending backward, not reaching anterior margin of the eye; lateral line complete; posterior chamber of air bladder degenerated; and fin differences. Triplophysayaluwang Lan, Liu, Zhou & Zhou, sp. nov. is distinguished from other hypogean species of the genus Triplophysa by having a combination of the following characteristics: body naked, scaleless, with irregular pale and dark brownish brown markings, except ventrally; eyes reduced, diameter 4.6-6.1% of head length; pelvic-fin tip reaching anus; tip of pectoral fin not reaching to pelvic fin origin; anterior and posterior nostrils closely set, with anterior nostril elongated to a barbel-like tip; tip of outrostral barbel extending backward, not reaching to anterior margin of the eye; lateral line complete; posterior chamber of air bladder degenerated; and fin differences. Mitochondrial Cyt b revealed relatively small genetic differences, 1.4-2.0%, between the two new species and close relatives. Nuclear gene RAG1 indicated that the two new species possessed unique haplotypes with multiple linking mutations. This study emphasizes the importance of utilizing nuclear genes to identify new species in rapidly speciation cave species, with small genetic differences due to mitochondrial introgression occurring interspecies.

A new species of Melanorivulus (Cyprinodontiformes: Rivulidae) from headwaters of the Taquari River basin, Paraguay River basin, central Brazilian Cerrado

ABSTRACT A new species of Melanorivulus is described from the upper Taquari River drainage, upper Paraguay River basin, in the Western Brazilian Cerrado. This species is a member of the Melanorivulus pictus group, endemic to the central Brazilian plateau and adjacent areas. Melanorivulus terena, new species, is endemic to the Paraguay River basin and is possibly closely related to other species endemic to the upper Paraná River basin, such as M. linearis, M. interruptus and M. egens, by the absence of distinctive dark marks on the humeral region in males. The new species is distinguished from all other species of the M. pictus group by a distal margin of unpaired fins and pelvic fin that is orangish red, and by the number of scales in the transversal series. The possible close relationship between the new species and other species recorded in the Paraná River basin may indicate an ancestral interconnection of the headwaters of the Rio Taquari (Paraguay River basin) with the headwaters of the upper Rio Verde and Rio Pardo (Paraná River basin). http://www.zoobank.org/urn:lsid:zoobank.org:pub:8B0027D9-8346-4ADB-AE9E-8C770823B8D9

Integrative taxonomy reveals a new species of Characidium (Characiformes: Crenuchidae) shared by tributaries of upper Tapajós and Xingu river basins, Brazil

AbstractA new species of Characidium shared by adjacent tributaries of the upper portions of the Tapajós and Xingu river basins is described. Characidium varii, new species, can be distinguished from the congeners by having distinctly speckled pattern of colouration, including dark blotches on cheek and ventral surface of head, by having conspicuous dark marks on fins, along with a single row of dentary teeth, isthmus completely covered by scales, 14 circumpeduncular scales, and swimbladder reduced. Molecular data reinforce the validation of the new species. The distribution of C. varii supports the hypothesis of the existence of a faunistic mixing between Tapajós and Xingu river basins, corresponding to the region of the Serra do Cachimbo and surrounding areas, previously proposed in the literature.

A new glassfrog of the genus Centrolene (Amphibia, Centrolenidae) from the Subandean Kutukú Cordillera, eastern Ecuador

We describe a new species of Centrolene from the Subandean Cordillera of Kutukú in southeastern Ecuador. The new species differs from all other glassfrogs by the combination of the following characters: presence of processes of vomers but without vomerine teeth; humeral spines in males; dorsum green with light green dots and without dark marks; dorsal skin with abundant tubercles; all visceral peritonea translucent (except for pericardium); and small body size (snout-vent length 21.5–21.9 mm in adult males). The new species is sister to Centrolene camposi from the Western Cordillera of the Andes of southwestern Ecuador, and together they form a clade with C. condor from the Subandean Cóndor Cordillera in southeastern Ecuador. Our time tree suggests that the new species originated at the end of the Pliocene. In addition, we present new information for C. zarza, expanding its geographic range across the southeastern Andes and the Kutukú and Cóndor cordilleras, amending its definition and diagnosis, and offering new information on its natural history and extinction risk. We also discuss the taxonomic status of Ecuadorian populations reported as C. huilensis and conclude that they are C. muelleri based on their close phylogenetic relationships and morphological similarity to samples of C. muelleri from Peru. Centrolene huilensis is a valid species and not closely related to C. muelleri.

Sinobdella longitubulus, a new species of spiny eel (Pisces, Mastacembelidae) from the Zhu-Jiang Basin, with a note on the type locality of S. sinensis (Bleeker, 1870)

The spiny eel genus Sinobdella belongs to the family Mastacembelidae of the order Synbranchiformes. Kottelat and Lim (1994) utilised Rhynchobdella sinensis as the type species to propose the genus. Currently, it contains a single species widespread in eastern and southern China and northern Vietnam. Sinobdella longitubulus, a new species of spiny eel, is here described from the Xi-Jiang of the Zhu-Jiang Basin in Guangxi Zhuang Autonomous Region, southern China. It differs from the single congeneric species S. sinensis in having a more or less white-brown reticulated pattern on the flank, two tubular anterior nostrils longer than or equal to the rostral appendage, an anal fin heavily mottled with dark brown markings and white spots and bearing a narrow white distal margin; shorter pre-anal length; and fewer abdominal vertebrae. The validity of this new species is corroborated by its monophyly recovered in a COI gene-based phylogenetic analysis and its significant sequence divergence with S. sinensis. A note on the type locality of S. sinensis is also given; its type specimen is possibly from mountain streams of Jiangxi Province, in the lower Chang-Jiang Basin.

A new mountain pitviper of the genus Ovophis Burger in Hoge & Romano-Hoge, 1981 (Serpentes, Viperidae) from Yunnan, China

Based on a molecular phylogenetic analysis and morphological comparison, a new species of mountain pitviper, Ovophis jenkinsisp. nov., is described. The new species was collected in Yingjiang County, Yunnan Province, China. It can be distinguished from congeneric species by the following characters: (1) internasals in contact or separated by one small scale; (2) second supralabial entire and bordering the loreal pit; (3) dorsal scales in 23 (25)–21 (23, 25)–19 (17, 21) rows; (4) 134–142 ventrals; (5) 40–52 pairs of subcaudals; (6) third supralabial larger than fourth in all examined specimens of Ovophis jenkinsisp. nov.; (7) deep orange-brown or dark brownish-grey markings on dorsal head surface; (8) background color of dorsal surface deep orange-brown or dark brownish-grey; (9) both sides of dorsum display dark brown trapezoidal patches; (10) scattered small white spots on dorsal surface of tail.

Bridging the gap: A new species of arboreal Abronia (Squamata: Anguidae) from the Northern Highlands of Chiapas, Mexico.

The mountain forests of Middle America are renowned for their endemic biodiversity, and arboreal alligator lizards (genus Abronia) are high-profile vertebrates endemic to this region. In this work, we describe a new species of arboreal Abronia that is known only from the type locality in the Northern Highlands of Chiapas, Mexico. The new species is diagnosed from all other members of the genus Abronia by the following combination of characters: lack of protuberant or spine-like supra-auricular scales, lack of protuberant or casque-like posterolateral head scales, dorsum of head pale yellow with distinct dark markings, 35-39 transverse dorsal scale rows, lateralmost row of ventral scales enlarged relative to adjacent medial row, and dorsum brown with darker crossbands that are sometimes reduced to rows of spots. We provisionally include the new species in the subgenus Lissabronia based on genomic and morphological evidence, but our results also suggest a close relationship to the subgenus Abaculabronia. The new species is geographically separated from the nearest Lissabronia and Abaculabronia species by the lowland Central Depression of Chiapas. Ongoing habitat loss and other factors imperil the new species, leading us to propose its listing under multiple threatened species frameworks. Because the Northern Highlands have poor coverage of protected areas, we briefly comment on the potential of this new species for stimulating conservation in the region.

고구려 암문토기의 기원 연구

This paper is to examine the origin of dark burnished marks, a distinctive feature of Koguryo Pottery. Dark burnished marks is a decorative pattern in which the surface of pottery with intricate designs using a sturdy tool, It is primarily found in pottery of northern ethnic groups such as Xiongnu, Xianbie, Koguryo, Balhae, and Khitan. In Koguryo, dark burnished marks appeared in the late mid-period when the use of fine clay became widespread. It is characterized by relatively simple pattern motifs like horizontal lines, square shapes, vertical lines, and continuous ring patterns, inscribed in various locations including the body of pottery. It is primarily found in relatively large and medium-sized vessels, such as jar, large jar, steamer, and large bowl. Dark burnished marks is not a decorative technique that originated in Koguryo. It was also found in Xiongnu pottery, which preceded Koguryo, as well as in the Three Yan or Northern Wei dynasty of pottery, which had close relationships with Koguryo. Xiongnu pottery was popular in the northern steppes, including Mongolia, from the 2nd century BC to the 1st century AD. Therefore, it is not directly related to the origin of dark burnished marks in Koguryo pottery. The Xiongnu had a significant influence on the development of Xianbei pottery culture. Dark burnished marks has been confirmed in Xianbei pottery from the 3rd century. It is difficult to establish a direct relationship between the pottery of Koguryo in the middle period and that of the Three Yan. This is due to differences not only in basic forms but also in aspects such as the types of vessels with dark burnished marks, the preferred locations of the application, and the preference for patterns. However, it is reasonable to understand the origin of dark burnished marks on Koguryo pottery in the context of the material culture exchange between Koguryo and the Murong Xianbei, who constantly competed for control of the Liaodong region from the 4th to the early 5th centuries. This is also the case with the introduction of mural tombs in Koguryo. It is likely that dark burnished marks of Koguryo pottery was the result of selective adoption of some elements, such as
 the ark burnished marks technique that has been passed down from the Xiongnu, rather than the formal elements of pottery, in the context of various forms of cultural exchange with the Three Yan.

Species in Disguise: A New Species of Hornshark from Northern Australia (Heterodontiformes: Heterodontidae)

A new species of hornshark is described from northwestern Australia based on six whole specimens and a single egg case. Heterodontus marshallae n. sp. was previously considered to be conspecific with H. zebra from the Western Pacific. The new species differs from H. zebra in the sequence of its NADH2 gene, several morphological characters, egg case morphology and key coloration features. Despite the coloration being similar between H. marshallae n. sp. and H. zebra, i.e., pale background with 22 dark brown bands and saddles, they differ consistently in two key aspects. Firstly, the snout of H. marshallae n. sp. has a dark semicircular bar, usually bifurcated for most of its length vs. a pointed, triangular shaped dark marking in H. zebra. Secondly, H. zebra has a dark bar originating below the posterior gill slits and extending onto anterior pectoral fin, which is absent in H. marshallae n. sp. The Heterodontus marshallae n. sp. is endemic to northwestern Australia and occurs in deeper waters (125–229 m) than H. zebra (0–143 m).

A new species of Andean frog of the genus Phrynopus (Anura: Strabomantidae) from southeastern Peru.

The Cordillera de los Andes is one of the most important regions for biodiversity. Among amphibians, many endemic species of terrestrial-breeding frogs have recently been discovered. Herein we describe Phrynopus sancristobali from the Andes of southeastern Peru based on molecular and morphological data. The new species is known from the ecotone between humid puna and montane forest at 3910 m a.s.l. on the left side of the Apurímac Valley in the Department of Ayacucho. The new species differs from congeners by having dorsum bearing pustules and light brown with dark brown reticulations surrounding the areolas, and coloration consisting of flanks golden brown with gray or dark brown marks, palms pale orange, soles deep orange, toes IV and V dark brown in dorsal and ventral view, belly cream to yellow with gray to light brown marks, and groin and throat deep orange. The snout-vent length (SVL) is 20.7 and 22.2 in two females, and 19.5 mm in one male. According to our phylogeny inferred using Maximum Likelihood with a concatenated dataset of three mitochondrial and two nuclear genes, P. sancristobali is sister taxon of P. apumantarum, recently described from Department Huancavelica. Our description extends the known geographic range of Phrynopus 73 km to the south, and P. sancristobali is the only species in the genus known to occur south of the Mantaro River, whose deep valley is hypothesized to be a biogeographic barrier for high-Andean organisms. The discovery of P. sancristobali confirms the high levels of endemism and beta diversity of Phrynopus in the moist puna grasslands and montane forests of the high Andes of Peru, and suggests that further work will reveal the presence of additional species in southern Peru.

Experimental Analysis on Polypropylene Moulded Part for Performance of Laser Printing

Now a day plastic injection molding is widely used process to manufacture engineering product and consumer goods typically thermoplastic is combined with rubber or another thermoplastic like RM master batch is used to add color to the Molded part also the laser marking on plastic part and their printing cut, faint issue observed. The aim of the study was to Optimize the injection parameters and processing condition for the laser performance on polypropylene part. To achieve enhanced dark laser marking on polypropylene, the process parameter of plastic injection molding and the Raw material master batch mixing parameter successfully prepared this laser–sensitive composite consisted of a high Laser induced carbonization rate.in plastic injection molding use of raw material and mixing of master batch is considerable factor for faint laser marking. Because of laser does not respond well on carbonization added material, it was evident that master batch having the carbon properties is much more responsible for faint laser marking issue on polypropylene material part. The effect of laser beam interaction (Nd: YVO4) with selected operational parameters on the Quality of graphical features obtained on the surface of polypropylene-molded pieces with different surface Textures (variable parameters of the surface layer). Polypropylene test specimens were injection molded using original injection molds Products with variable end parameters determined by the position of the cavity circle can be identified. The layout of the laser function, the beneficiary texture of the molded piece, the molded color and the support of the marking piece allow the evaluation of the graphic symbol performance by means of laser marking of the type of master back in their rendering relationship. Marked evaluation criteria were adopted for the project.

An unusual ‘speckled’ puff adder Bitis arietans (Reptilia: Squamata) from central South Africa

AbstractA young female puff adder (Bitis arietans) with an unusual ‘speckled’ colour pattern was recently found in Bloemfontein, central South Africa. The snake was grey‐brown dorsally with numerous scattered dark markings and very occasional pale flecks, but it lacked the backwards‐pointing, dark‐edged, pale chevrons that characterise this common, widespread and highly venomous species. It also lacked any stripes or bands, but the head was lightly patterned. Although various rare colour morphs have been reported for this species, this is the first record of a puff adder with a ‘speckled’ dorsal pattern.

Description of a new subterranean breeding brown frog (Ranidae: Rana) from Japan

Taxonomic relationship of two forms of Rana tagoi, a brown frog endemic to Japan, is assessed. Based on morphological and bioacoustical comparisons, as well as molecular analysis, we consider the large-form-lineage as true R. tagoi, and describe the small-form-lineage as Rana kyoto sp. nov. The new species and R. tagoi occur sympatrically in many places around the Kinki District, although the former can be distinguished from the latter by smaller body size, more developed dark marking on ventral side of legs, less developed toe webs, and advertisement call with fewer notes and lower dominant frequency. Rana kyoto sp. nov. sympatric with R. tagoi show smaller body size than the allopatric ones, whereas R. tagoi sympatric with the new species show larger body size than the allopatric ones, suggesting that the character displacement of body size occurs between the two species around the type locality of Rana kyoto sp. nov.

Explant analysis of a Discocerv cervical disc: A case study for a ceramic-on-ceramic cervical disc

Explant analyses are key to better understanding the effectiveness of medical implants in replacing natural joints. For the first time, an explanted Discocerv cervical disc was examined. The implant utilised the articulation of a caudal zirconia cup (inferior component) and a cephalic alumina head (superior component). The articulating surface of the superior alumina head had an average surface roughness of 0.016 ± 0.003 μm (Sa) and the articulating surface of the inferior zirconia cup had an average surface roughness of 0.015 ± 0.002 μm (Sa). Both articulating surfaces had negative skewness, indicating the removal of local peaks. The difference between the average surface roughness of the components was not significant (p-value: 0.741). Dark grey marks were observed on both of the articulating surfaces, which were found to be adhered titanium debris that was generated due to component impingement. This titanium debris may explain the small amount of metallosis that was reported at explantation. Some transfer of zirconium to the alumina articulating surface was also seen.

Modeling the Transmission Dynamics of COVID-19 Among Five High Burden African Countries.

BackgroundToday, coronavirus disease-19 has left a permanent dark mark on the history of human beings. The ongoing global pandemic outbreak of COVID-19 has spread to 58 African countries, with over 6.07 million confirmed cases and over 151,412 deaths. The five high burden African countries are South Africa, Morocco, Tunisia, Ethiopia, and Libya, with case fatality rates (CFR) of nearly 0.15%, 0.042%, 0.22%, 0.006%, and 0.086%, respectively. This is why the research aims to adequately understand the transmission dynamics of the virus and its variants in five high-burden African countries.MethodsOur study is a deterministic model, where the population is partitioned into five components on the epidemiological state of the individuals. We presented a year-structured susceptible, infected, mild severs, critical severe, and recover (SIMCR) compartmental model of COVID-19 disease transmission with incidence rate during the pandemic period.ResultsThe number of susceptible individuals increased by 30,711,930 in South Africa, 5,919,837 in Morocco, 3,485,020 in Tunisia, 7,833,642 in Ethiopia, and 2,145,404 in Libya in the next 3 decades with compare to the unvaccinated population and the number of infected individuals decreased by 30,479,271 in South Africa, 19,809,751 in Morocco, 3,456,406 in Tunisia, 7,761,993 in Ethiopia, and 2,125,038 in Libya.ConclusionSIMCR model is used to describe the transmission of COVID-19 among five high-burden African countries. For the next 30 years, we will have around 86 million infected individuals and millions of death only in those five African countries. To reduce those problems, vaccination is the best and most effective mechanism. So vaccinating half of the populations in those countries helps to control and reduce the transmission rate of COVID-19 in Africa for the next 30 years. This leads to preventing 17,212,405 people from becoming infected and millions of deaths being reduced in those five high-burden African countries.

The Refugee, an Anguish of Million

"The partition of India left a dark and ineradicable mark in the history of India, which created painful anguish among millions of people, especially in Bengal and Punjab. The sea change about the independence of India made an inexplicable turn of events in the annals of history. The spirit of brotherhood and the generation of human values collapsed suddenly with the advent of independence in the country. This socio-political aspect touched upon the playwright Asif Currimbhoy in making the play The Refugee. Currimbhoy tried to generate compassion through human suffering in and around Bengal, especially in Calcutta. The present play, The Refugee, draws the parallel between Yassin, the young refugee from East Pakistan, and Sen Gupta, a refugee to India from East Pakistan many years earlier. In the beginning, Yassin is cloudy and undecipherable with his indecisive acts towards the cause. On the other hand, Sen Gupta crossed borders many years earlier as a refugee, and he is now well settled in India with his friendly attitude and hardworking nature. Surprisingly, both are at present unperturbed towards the worsened situation of millions of refugees, who are in the camps and outside of the house of Sen Gupta and who need to be addressed by the concern. This play is about the anguish of millions of refugees. It is an act of resolution of dilemma in irony about the influx of refugees growing in number."

On the occurrence of Scinax kennedyi (Pyburn, 1973) (Anura, Hylidae) in the state of Roraima, northern Brazil

Scinax comprises 129 species of treefrogs divided between the S. ruber and the S. catharinae clades. The S. rostratus group belongs to the S. ruber clade, and comprises ten species characterized by having a dark interorbital triangular mark, tubercle on the heel and by vocalizing in a head-down position. Within this group, S. kennedyi and S. rostratus are similar in that they are the only species that do not possess a row of tubercles on the lower jaw. In spite of their morphological similarity, they can be distinguished from each other by male size and advertisement call traits. Scinax kennedyi was described from eastern Colombia, and occurs from central Colombia to western Venezuela, whereas S. rostratus was described from northern Venezuela, and is thought to occur from Panama to eastern Venezuela, and in the Brazilian state of Pará. A recent study reported a population identified as S. rostratus from the state of Roraima, in northern Brazil. However, this population presented substantial differences in male size and advertisement call in comparison to the described for this species, and apparently it best fits the diagnosis of S. kennedyi instead. In view of this, the present study aimed to clarify the identity of this Brazilian population through a reassessment of its specimens and call recordings, together with the examination of data of S. kennedyi from its type locality, and of S. rostratus from northern Venezuela. Acoustic and morphological analyses revealed that specimens from northern Brazil were indeed misidentified as S. rostratus, and hence they were herein reassigned to S. kennedyi. The study therefore demonstrates the occurrence of S. kennedyi in Brazil, extending its distribution ca. 1,120 km east by south from its type locality, and ca. 844 km east-southeast from its easternmost previous record. With this reassessment, a clearer acoustic diagnosis between S. kennedyi and S. rostratus was provided, as well as further data on fine-scale temporal traits of their calls.

Revisions of Holocnemus and Crossopriza: the spotted-leg clade of Smeringopinae (Araneae, Pholcidae)

The genera Holocnemus Simon, 1873 and Crossopriza Simon, 1893 are revised. Together with Stygopholcus Kratochvíl, 1932 (revised recently) and the newly described genus Maghreba gen. nov., they constitute the spotted-leg clade within the northern clade of Smeringopinae. Males and females in this group are characterized by dark marks on the leg femora and tibiae. The native area of the spotted-leg clade ranges from northern Africa and the Mediterranean to Central Asia and NW India. A morphological cladistic analysis suggests that Holocnemus is paraphyletic while Crossopriza is monophyletic, but morphology seems only partly adequate to resolve phylogenetic relationships convincingly. The genus Holocnemus includes four species, all of which are redescribed: H. pluchei (Scopoli, 1763); H. reini (C. Koch, 1873) comb. nov. (transferred from Pholcus); H. caudatus (Dufour, 1820); and H. hispanicus Wiehle, 1933. The genus Maghreba gen. nov. includes eight species from NW Africa: M. aurouxi (Barrientos, 2019) gen. et comb. nov. (transferred from Holocnemus; redescribed, female newly described) and seven newly described species. The genus Crossopriza includes six previously described species (of which five are redescribed), and 18 newly described species. The Madagascan C. nigrescens Millot, 1946 is synonymized with C. lyoni (Blackwall, 1867). All new species are described on the basis of both sexes.

A new species of the congrid eel genus Ariosoma (Anguilliformes: Congridae) from the south-west coast of India.

A new congrid eel species, Ariosoma maurostigma sp. nov., is described on the basis of 24 specimens collected from the deep-sea trawl by-catch, Kalamukku Fishing Harbour, off Kerala, Arabian Sea. The new species differs from all other congeners in having the following combination of characters: dark mark or spot on the posterodorsal margin of the eyes; dorsal surface of head with two faint darkish bands across the anterior and posterior margin of the eye; origin of the dorsal fin before the pectoral-fin base; short vomerine teeth patch, ST pores 3, 1 median pore and 1 lateral pore on each side just behind the median pore; preanal vertebrae 47-51; precaudal vertebrae 54-57; total vertebrae 136-142; total pores 129-134. The phylogenetic analysis reveals that the new species is closely related to Ariosoma melanospilos and Ariosoma anale, with divergences of 13.8% and 14.9%, respectively.

A new species of Goeldichironomus Fittkau, 1965 from Argentina (Chironomidae, Chironominae).

Goeldichironomus pampeanus n. sp. is described and figured as male, pupa and larva based on reared specimens from Buenos Aires province in Argentina. The species can be separated from its congeners on the combination of the following characters: male without dark markings on thorax and an evenly curved superior volsella projecting mesad; pupa with extensive shagreen on tergites IIVI, sternites with fine shagreen only without transverse bands of points, dorsal setae on tergite IX extending beyond anal lobe and basal ring of thoracic horn kidney-shaped; larva with bifid premandible, mentum with 6 pairs of lateral teeth, anterior pair of ventral tubules simple, labral sclerite 1 intact and labral sclerite 2 consolidated on the inner side.