Leafy Vegetables Research Articles

Associations of vegetable and fruit intake with cognitive function and its decline: Two longitudinal studies

ObjectivesPrevious studies suggested protective associations of vegetables and fruits (VF) intake with cognitive function, but evidence on specific types of VF was insufficient. MethodsThe current study included 4066 participants from 1997 to 2006 in the China Health and Nutrition Survey (CHNS) and 6170 participants from 2013 to 2020 in the Health and Retirement Study (HRS). Dietary intake (using 3-day 24-h dietary recalls in CHNS and food frequency questionnaire in HRS) and cognitive function (using the Telephone Interview for Cognitive Status-Modified, TICS-m) were measured. Linear mixed-effects models were used to estimate the beta coefficients (β) and the 95% confidence intervals (CI) to evaluate the association of VF with cognitive function (z-score) and its decline. ResultsHighest intake of total VF was associated with better cognitive function and slower cognitive decline. Differences in cognitive function z-score between the highest and lowest tertiles of VF consumption were 0.039 (95% CI: 0.002, 0.076) for CHNS and 0.063 (95% CI: 0.026, 0.100) for HRS. The corresponding differences in annual cognitive decline were 0.011 (95% CI: 0.002, 0.021) and 0.012 (95% CI: 0.003, 0.020) units respectively. Vegetables and fruits showed independent associations with cognitive function and its decline. In specific VF subgroups, when comparing the highest to the lowest tertile intake, cruciferous vegetables (β = 0.058, 95% CI: 0.017, 0.100 in CHNS and β = 0.067, 95% CI: 0.032, 0.101 in HRS) and green leafy vegetables (β = 0.036, 95% CI: −0.001, 0.073 in CHNS and β = 0.082, 95% CI: 0.046, 0.117 in HRS) was associated with better cognitive function in both cohorts. Similarly, higher intake of dark-colored vegetables (β = 0.019, 95% CI: 0.008, 0.030 for red/yellow vegetables in CHNS and β = 0.004, 95% CI: 0.001, 0.007 for green leafy vegetables in HRS) were associated with slower cognitive decline in subsequent years. Moreover, rigorous sensitivity analyses confirmed the stability of the results. ConclusionsOur findings support the potential beneficial roles of VF, especially cruciferous vegetables, green leafy vegetables, and red/yellow vegetables, in maintaining cognitive function and slowing cognitive decline in middle-aged and older adults.

Optical, thermal, FTIR, SEM-EDX and 1H NMR analysis of Chenopodium album (Bathua) powder prepared using different drying techniques

Chenopodium album (L.) is an underutilized agricultural crop consumed as a leafy vegetable in Asian countries. Although perishable and cultivated in a specific season, it contains nutrients and phytochemicals in abundance. Preservation by employing drying techniques helps in their utilization in the off-season for better food security. In the present study, Chenopodium album leaves were dried using sun, tray, and freeze drying. The different powder samples were characterized on the basis of their physicochemical, optical, and thermal properties. The water activity of powder samples ranged between 0.29 and 0.56. The highest color retention (L* value 47.21) and lowest particle size (11.5 µm) was observed in freeze dried samples. Fourier-transform infrared spectroscopy and Nuclear magnetic resonance spectra confirmed the presence of several functional groups. Thermal analysis revealed the thermal degradation of powder due heat as depicted by change of state and loss of weight. Surface morphological study indicated that powder samples were flowable and element analysis showed presence of C, O, K, Ca, Mg, P, S element in powders.

Optimizing Sowing Density for Parsley, Cilantro, and Sage in Controlled Environment Production: Balancing Productivity and Plant Quality

Sowing density is a key management practice influencing productivity and quality of leafy greens and culinary herbs grown in controlled environments. However, research-based information on optimal density is limited for many culinary herbs. This greenhouse study aimed to quantify sowing density impacts on biomass output, individual plant growth, and morphological traits in hydroponically produced ‘Giant of Italy’ parsley (Petroselinum crispum), ‘Santo’ cilantro (Coriandrum sativum), and sage (Salvia officinalis). Seedlings were grown in phenolic foam cubes with 1, 5, 10, 15, or 20 seeds per cell, transplanted into an ebb-and-flow hydroponic system in a glass-glazed greenhouse with 23 °C target average daily temperature, 16-hour photoperiod, a target daily light integral of 13 mol·m−2·d−1, and harvested at 16 to 28 d after transplanting depending on species. ‘Giant of Italy’ parsley and ‘Santo’ cilantro fresh weight per cell increased quadratically by 274% (57.3 g) and 305% (19 g), respectively, as sowing density increased from 1 to 15 seeds per cell, then plateaued as density further increased. Sage fresh weight plateaued at 10 seeds per cell with an increase of 225% (29.2 g) compared with 1 seed per cell. Cilantro and sage dry weight per cell plateaued at 14 and 8 seeds per cell, respectively, and parsley dry weight quadratically increased as sowing density rose up to 20 seeds per cell. Although fresh and dry weight increased, individual plant height, stem diameter, and individual plant dry weight exhibited linear or quadratic declines as sowing density increased, indicating higher sowing densities restricted individual plant growth. In summary, as sowing density increased, fresh and dry weight per cell generally increased but individual plant quality decreased. For the greatest fresh and dry weight, 20, 18, and 10 seeds per cell should be sown for parsley, cilantro, and sage, respectively. However, to balance fresh weight and crop quality, our results suggest sowing density (seeds per cell) targets of 16 seeds for parsley, 18 seeds for cilantro, and 10 seeds for sage.

Effects of the Mixed Seeding Rate of Milkvetch on Fertilizer Substrate, Growth, and Carotenoid Levels of Baby Leaf Vegetables in Vertical Indoor Farming

During this study, an indoor experiment was conducted to determine the effect of mixed seeding rates of legumes used as green manure on the substrate fertilizer, growth characteristics, and bioactive compounds of baby leaf vegetables. The mixed seeding treatment was designed for milkvetch (Astragalus sinicus L.), tatsoi (Barassica rapa L.), kale (Brassica oleracea var. sabellica L.), and spinach (Spinacia oleracea L.) using five rates for each. Accordingly, a total of 15 treatments (3 baby leaf species × 5 mixed seeding rates) were constructed using a randomized complete block design with three replications for each treatment. During the baby leaf vegetable harvest, we evaluated the macronutrient levels (nitrogen, phosphorus, and potassium) in the substrate as well as the growth parameters and carotenoid contents. The substrate in the treatment mixed with milkvetch showed significantly higher levels of nitrogen, phosphorus, and potassium compared with those of tatsoi and kale sown alone (P ≤ 0.05). However, there were no significant differences in macronutrients observed in substrate-sown spinach with or without the milkvetch mixture. The growth and carotenoid levels of each baby leafy vegetable sown alone were significantly higher than those of each baby leafy vegetable sown with the mixed seeding treatment (P ≤ 0.05). Sowing the milkvetch–vegetable mixtures did not result in a significant increase in the growth and carotenoid levels of the three baby vegetables. The results showed that planting milkvetch with tatsoi and kale had a significant impact on substrate fertilization. However, regarding short-term vertical indoor farming, the growth and carotenoid responses of the three greens may be different. Nonetheless, we still believe that the combined interactions of legumes can provide long-term benefits by enhancing the biological functionality of the growing medium for balanced indoor agriculture production.

Hydroponic Multilayer Farming Automation with IOT

Abstract: This study introduces a novel high-tech, small-scale hydroponic system utilizing Internet of Things (IoT) technology for cultivating leafy vegetables. It employs low-cost materials and sensors for remote monitoring and process automation, addressing challenges like rural population decline and adverse climate changes. Experimental investigations focused on lettuce cultivation demonstrated the system's ability to maintain optimal environmental parameters, achieving 75% seed germination. Key benefits include easy management, remote monitoring, and suitability for small to medium-sized vegetable growers, enabling sustainable year-round farming of leafy greens indoors.

Non-Pathogenic Pythium Induced the Resistance in Hydroponic Lettuce Against Root Rot Disease

Hy droponics is a common soilless type of culture. It is clean and gives high-yield crop production. Lettuce is the most widely grown leafy vegetable in hydroponics. The Pythium species causes root rot, a serious disease in lettuce. In this study, we investigated an effective elicitor to promote resistance in lettuce after pathogen infection. To evaluate the plant defense gene response profile, the expression stability of five candidate genes was studied using RT-qPCR. The LsPR1 and LsLTC2 gene expression were higher in lettuce leaves treated with non-pathogenic Pythium PyASR23 than in roots compared to untreated plants. To assess the disease, the rate of relative gene expression of LsLTC2, LsPR-1b like, LsERF, LsAOS, and LsDEF was associated with Pythium root rot pathogenesis. As a result, it was revealed that alterations in these gene expressions were a defense response to Pythium infection, which increased after pre-treatment with various elicitors for 24 hours. When the chemically stimulated lettuce was infected with pathogenic PySR31, the expression of these genes increased dramatically. These results suggested that non-pathogenic PyASR23 might be employed as an elicitor in lettuce roots and contribute to disease control by activating plant defense gene expression against the Pythium pathogen.

Wild edible vegetables of ethnic communities of Mizoram (Northeast India): an ethnobotanical study in thrust of marketing potential

BackgroundAssessment of wild edible vegetables (WEVs) from the ethnobotanical approach is a significant key to understanding indigenous knowledge systems. The available literature has revealed a tremendous decline in knowledge of WEVs over the last few decades. The main purpose of this study was to document and analyse the traditional knowledge of WEVs among the two major ethnic groups of Mizoram regarding their use and the diversity associated with the importance of traditional medicines. Secondly, a market survey will be conducted to determine the status of available WEVs.MethodsThis study conducted an ethnobotanical survey among 72 informants through semi-structured interviews and questionnaires besides direct field observations. The documented data were quantitatively analysed using various ethnobotanical indices, including Informant’s consensus factor (ICF), Fidelity level value (Fl), and Direct matrix ranking (DMR). A marketing survey was conducted in the Bara Bazar, Mizoram's most prominent local market. A total of 38 vendor informants were interviewed to observe and collect the price of commonly sold WEVs.ResultsA total of 70 WEVs, distributed in 58 genera under 36 families, were documented and identified. Of these, 33 WEVs were of medicinal importance. Leafy vegetables were documented as the most frequently consumed parts (55.71%). The majority (44.29%) of the plants documented were consumed in fried form. The highest level of agreement among informants for food used categories was observed for plants combined with dry fish (ICF = 1). The Informant’s consensus factor (ICF) of disease categories ranges from 0.75 to 1, with the highest being reported for convulsion (ICF = 1), sleep inducer (ICF = 1), and antiseptic (ICF = 1). Picria fel-terrae was the most preferred plant for hypertension treatment (100% FL). Direct matrix ranking (DMR) indicated that Dysoxylum excelsum was highly utilized by the inhabitant for multipurpose species (DMR = 64). Jaccard similarity index (JI) between the two ethnic groups was revealed at 1.26. Forty-seven WEVs were found to be commercialized in the Bara Bazar market, Aizawl, with a price range from 0.1 to 2.4 USD. Ensete superbum was reported as near threatened per the IUCN Red List of Threatened Species.ConclusionThis work highlighted the importance and rich diversity of WEVs in Mizoram, which are presently used among different age groups for food and medicine. Informants have good knowledge of WEVs, which was shared to a great extent among the inhabitants; this legacy of traditional culture must be conserved. This study further suggests a priority setting for conserving multipurpose WEVs in human-inhabited sites, investigating the recorded species' nutritional properties and pharmacological activities.

Uptake of tire-derived compounds in leafy vegetables and implications for human dietary exposure

IntroductionTire and road wear particles are one of the most abundant types of microplastic entering the environment. The toxicity of tire and road wear particles has been linked to their organic additives and associated transformation products. Tire and road wear particles, and associated tire-derived compounds are introduced to the agricultural environment via atmospheric deposition, irrigation with reclaimed wastewater, and the use of biosolids (treated sewage sludge) as fertilizer. In the agricultural environment, these tire-derived compounds could be taken up by edible plants, leading to human exposure.MethodsSixteen tire-derived compounds were measured in twenty-eight commercial leafy vegetable samples from four countries. Based on the results, the estimated daily intake of these tire-derived compounds was calculated due to leafy vegetable consumption based on local diets under a mean and maximum concentration scenario.ResultsIn commercial leafy vegetables, six tire-derived compounds were detected: benzothiazole (maximum concentration—238 ng/g dry weight), 2-hydroxybenzothiazole (maximum concentration—665 ng/g dry weight), 1,3-diphenylguanidine (maximum concentration—2.1 ng/g dry weight), N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD, maximum concentration—0.4 ng/g dry weight), N-Isopropyl-N-phenyl-4-phenylenediamine (IPPD, maximum concentration—0.1 ng/g dry weight), and N-phenyl-N-cyclohexyl-p-phenylenediamine (CPPD, maximum concentration—0.3 ng/g dry weight). At least one compound was present in 71% of samples analyzed. The estimated daily intake for 1,3-diphenylguanidine ranged from 0.05 ng/person/day in the mean scenario to 4.0 ng/person/day in the maximum scenario; benzothiazole ranged from 12 to 1,296 ng/person/day; 6PPD ranged from 0.06 to 2.6 ng/person/day; IPPD ranged from 0.04 to 1.1 ng/person/day; CPPD ranged from 0.05 to 2.6 ng/person/day.DiscussionStatistical analyses did not reveal correlation between known growth conditions and tire-derived compound concentrations in the leafy vegetable samples. The estimated daily intake via leafy vegetable consumption was generally lower than or comparable to the estimated daily intake via other known sources. However, we show that tire-derived compounds are taken up by foodstuff, and exposure might be higher for other produce. Future studies are needed to uncover pathways of tire-derived compounds from road to food, assess the exposure to transformation products, and investigate the biological effects associated with this exposure.

Effect of Intercropping with Leafy Vegetables on the Growth, Yield and Quality of Gladiolus Under Agro-Climatic Conditions of Prayagraj

Intercropping brings diversity of species in the cropping systems and is considered to make them more resilient against environmental stresses. It can significantly enhance the efficient use of land, water and solar energy besides assuring insurance against failure of one or the other crops. Therefore, an attempt was made to include leafy vegetable crops as intercrops in gladiolus cv. Novalux, for higher productivity and profitability per unit area. The experiment consists of seven treatments with three replications. From the present investigation, it was concluded that the intercrops significantly affected the performance of gladiolus. The intercropping combination T6: Gladiolus + Fenugreek gave the best results in terms of plant height (91.40 cm), number of leaves per plant (18.53), number of shoots per corm (4.93), days to spike initiation (64.33), spike length (73.93 cm), rachis length (49.47 cm), number of florets per spike (12.93), floret size (11.27), vase life (18.53 days), weight of corms (63.67g) and cormlets (6.77g), yield of corms (2385.00kg/ha) and cormlets (225.50kg/ha). The herbage yield per plot was recorded the highest in T3: Fenugreek sole (880.00. g), and intercropping combination of Gladiolus and fenugreek (T6) gave 665.83g. Based on the recent findings, intercropping of fenugreek with gladiolus can be recommended and expanded on a larger scale for additional yield.

Valuation of different organic and inorganic fertilizer combinations on the growth and yield of cabbage

abbage (Brassica oleracea var. capitata) is the most important winter leafy vegetable in the world and belongs to the Cruciferae family. An experiment was conducted at the Horticultural farm of Gauradaha Agriculture Campus, Tribhuwan University, Jhapa, Nepal from 26th December 2022 to 2nd April 2023 to evaluate the different organic and inorganic fertilizer combinations on the growth and yield of cabbage (Brassica oleracea var. capitata L. cv. Green coronet). The experiment was conducted as a randomized block design with 7 treatments replicated 3 times and the treatments are T1: 25-ton FYM/ha, T2: 25-ton PM/ha, T3: 25-ton FYM/ha + NPK (RDF), T4: 25-ton PM/ha + NPK (RDF), T5: 20-ton FYM/ha + 10-ton PM/ha + NPK (RDF), T6: 10-ton FYM/ha + 20-ton PM/ha + NPK (RDF) and T7: control. Significant variation was found among the treatments. The result showed treatment had a significant role in different parameters. T3- The highest number of leaves, T5- the highest (leaf area, weight with roots, net head yield, horizontal head diameter), T4-highest (plant height, gross yield), T6- highest (stem girth, vertical head diameter) was obtained. On the result basis T5 (20-ton FYM/ha + 10-ton PM/ha + NPK (RDF) can be adopted as sustainable crop production. This study not only provides valuable insights into optimizing cabbage production but also underscores the importance of tailored fertilizer combinations for sustainable agriculture. Furthermore, the findings contribute to the broader discourse on enhancing crop yield while minimizing environmental impact. By elucidating the efficacy of specific fertilizer blends, this research paves the way for more efficient and eco-friendly farming practices, thereby promoting food security and environmental sustainability on a global scale.

Domestic (re)infestation risk with the main vector Triatoma infestans increases with surrounding green vegetation and social vulnerability in the Argentine Chaco.

Chagas disease, caused by Trypanosoma cruzi, is still a public health problem in Latin America and in the Southern Cone countries, where Triatoma infestans is the main vector. We evaluated the relationships among the density of green vegetation around rural houses, sociodemographic characteristics, and domestic (re)infestation with T. infestans while accounting for their spatial dependence in the municipality of Pampa del Indio between 2007 and 2016. The study comprised sociodemographic and ecological variables from 734 rural houses with no missing data. Green vegetation density surrounding houses was estimated by the normalized difference vegetation index (NDVI). We used a hierarchical Bayesian logistic regression composed of fixed effects and spatial random effects to estimate domestic infestation risk and quantile regressions to evaluate the association between surrounding NDVI and selected sociodemographic variables. Qom ethnicity and the number of poultry were negatively associated with surrounding NDVI, whereas overcrowding was positively associated with surrounding NDVI. Hierarchical Bayesian models identified that domestic infestation was positively associated with surrounding NDVI, suitable walls for triatomines, and overcrowding over both intervention periods. Preintervention domestic infestation also was positively associated with Qom ethnicity. Models with spatial random effects performed better than models without spatial effects. The former identified geographic areas with a domestic infestation risk not accounted for by fixed-effect variables. Domestic infestation with T. infestans was associated with the density of green vegetation surrounding rural houses and social vulnerability over a decade of sustained vector control interventions. High density of green vegetation surrounding rural houses was associated with households with more vulnerable social conditions. Evaluation of domestic infestation risk should simultaneously consider social, landscape and spatial effects to control for their mutual dependency. Hierarchical Bayesian models provided a proficient methodology to identify areas for targeted triatomine and disease surveillance and control.

Growth response of pak choi (Brassica rapa L.) on different concentrations and intervals of eco-enzyme applications

Vegetable cultivation using eco-enzyme application is one way to improve its production. The study aimed to evaluate the growth response of pak choi using eco-enzyme in several concentrations and its interval. The experiment used a randomized complete block design with two factors (concentrations and interval of eco-enzyme applications). The results showed that the application of eco-enzyme with a concentration of 30 mL L-1 indicated an optimal growth stimulator in pak choi, but it was not significantly different as compared to 50 mL L-1. The application of eco-enzyme twice a week was able to stimulate plant growth including plant height, number of leaves, leaf area, shoot fresh weight, and root fresh weight. Furthermore, there was a linear correlation between leaf area and plant height, leaf number, shoot fresh weight, and root fresh weight that ranged from weak to strong levels. It is recommended to apply eco-enzyme at a concentration of 30 mL L-1 twice a week for optimal pak choi production. Keywords: correlation, leafy vegetable, green olericulture, organic fertilizer, plant booster

Dietary habits and self-reported health outcomes in a cross-sectional survey of health-conscious adults eating a plant-based diet.

Given the growing popularity of plant-based diets, this study investigated the dietary habits and self-reported health outcomes of health-conscious adults consuming plant-based diets. A cross-sectional online survey (n = 315) was distributed to members of Plant-Based Health Professionals UK, a community interest company. Dietary intake was assessed through a food frequency questionnaire. Data were summarised descriptively. Dietary habits among respondents following a whole food plant-based (WFPB) and vegan diet were compared using hypothesis tests. Respondents reported following a WFPB (61%), vegan (28%) and semi plant-based (11%) diet. Median time on current dietary pattern was 5 years. Daily or more frequent consumption was reported for the following foods: fruits 77%, berries 51%, green vegetables 48%, cruciferous vegetables 45%, other vegetables 64%, beans/legumes 41%, whole grains 62%, nuts and all seeds 63%. Consumption of ultra-processed foods and plant-based meat alternatives was low. About 93% of those on a WFPB or vegan diet supplemented with vitamin B12 and 61% with vitamin D. The median body mass index was 22.4 kg/m2. Fifty per cent of participants reported weight loss after adopting a plant-based diet, with a median loss of 6.4 kg. Thirty-five per cent reported reversing or improving an underlying health condition, and 15% were able to stop or reduce prescribed medication use as a result of dietary changes. This study suggests that a well-planned plant-based diet is achievable and sustainable in a community setting and can be associated with health benefits. How to best encourage such sustainable diets among the broad population requires further research.

Vitamin K2 in Health and Disease: A Clinical Perspective

Vitamins are essential organic compounds that vary widely in chemical structure and are vital in small quantities for numerous biochemical and biological functions. They are critical for metabolism, growth, development and maintaining overall health. Vitamins are categorised into two groups: hydrophilic and lipophilic. Vitamin K (VK), a lipophilic vitamin, occurs naturally in two primary forms: phylloquinone (VK1), found in green leafy vegetables and algae, and Menaquinones (VK2), present in certain fermented and animal foods and widely formulated in VK supplements. This review explores the possible factors contributing to VK deficiency, including dietary influences, and discusses the pharmacological and therapeutic potential of supplementary VK2, examining recent global clinical studies on its role in treating diseases such as osteoporosis, osteoarthritis, rheumatoid arthritis, cardiovascular disease, chronic kidney disease, diabetes, neurodegenerative disorders and cancers. The analysis includes a review of published articles from multiple databases, including Scopus, PubMed, Google Scholar, ISI Web of Science and CNKI, focusing on human studies. The findings indicate that VK2 is a versatile vitamin essential for human health and that a broadly positive correlation exists between VK2 supplementation and improved health outcomes. However, clinical data are somewhat inconsistent, highlighting the need for further detailed research into VK2′s metabolic processes, biomarker validation, dose–response relationships, bioavailability and safety. Establishing a Recommended Daily Intake for VK2 could significantly enhance global health.

Regional divergent evolution of vegetation greenness and climatic drivers in the Sahel-Sudan-Guinea region: nonlinearity and explainable machine learning

IntroductionThe vegetation dynamics of the Sahel-Sudan-Guinea region in Africa, one of the largest transition zones between arid and humid zones, is of great significance for understanding regional ecosystem changes. However, a time-unvarying trend based on linear assumption challenges the overall understanding of vegetation greenness evolution and of tracking a complex ecosystem response to climate in the Sahel-Sudan-Guinea region.MethodsThis study first applied the ensemble empirical mode decomposition (EEMD) method to detect the time-varying trends in vegetation greenness based on normalized difference vegetation index (NDVI) data in the region during 2001–2020, and then identified the dominant climatic drivers of NDVI trends by employing explainable machine learning framework.ResultsThe study revealed an overall vegetation greening but a significant nonlinear spatio-temporal evolution characteristic over the region. Trend reversals, i.e., browning-to-greening and greening-to-browning, were dominant in approximately 60% of the study area. The browning-to-greening reversal was primarily observed in the southern Sahel, Congo Basin north of the Equator, and East Africa, with a breakpoint around 2008, while the greening-to-browning reversal was mainly observed in West Africa, with a breakpoint around 2011. The sustained greening primarily took place in northern Sahel, Central African Republic and South Sudan; while sustained browning clustered in central West Africa and Uganda, mainly in agricultural lands. Furthermore, the combination of Random Forest (RF) algorithm and the SHapley Additive exPlanations (SHAP) method could robustly model and reveal the relationships between the observed trends in NDVI and in climatic variables, also detected by applying EEMD. The results suggested that air temperature and precipitation were the most important climatic drivers controlling the NDVI trends across the Sahel-Sudan-Guinea region. The NDVI trends were more likely to have negative correlations with solar radiation and vapor pressure deficit in arid areas, while they could have positive correlations in humid areas. The study also found that large-scale climate changes induced by sea surface temperature (SST) anomalies had strong relationships with trend reversals in vegetation greenness at a sub-continental scale. These findings advanced the understanding of the impacts of climatic drivers on vegetation greenness evolution in the Sahel-Sudan-Guinea region.

Influence of growth stages on the nutritional value of Momordica balsamina leaf powder

The nutritive mineral element concentrations of most African leafy vegetables (ALVs) are influenced by the plant’s growth stages. Momordica balsamina is an ALV in the Cucurbitaceae family, rich with nutraceutical and pharmaceutical properties, available in the plant at different growth stages. A greenhouse study was conducted to determine the responses of the nutritional value of M. balsamina leafy vegetable harvested at different growth stages. Six growth stages, namely, vegetative (reference), bud development, flower initiation, fruit set, fruit development and physiological maturity, served as treatments, with 10 replicates and arranged in RCBD. Treatments had highly significant (P ≤ 0.01) effect on the tested nutritional quality, namely, potassium (K), calcium (Ca), magnesium (Mg), zinc (Zn), iron (Fe) and phosphorus (P). Relative to the reference, K was the highest (1206 mg/L) at flower initiation stage, whereas the highest Ca (127.70 mg/L) and Zn (2.21 mg/L) concentrations occurred at the fruit development stage. At the physiological maturity stage, the highest Mg (25.19 mg/L), P (16.54 mg/L) and Fe (5.49 mg/L) concentrations, were recorded. Noticeably, during early growth stages, P, Fe and Zn were negatively reduced, when compared to K, Ca and Mg, which were increasing at every growth stages. In conclusion, the greatest accumulation of the tested mineral elements was mainly observed as from when the leafy vegetable started setting fruit until physiological maturity stage.

Impact of Plant Layout on Microclimate of Summer Courtyard Space Based on Orthogonal Experimental Design

With the rapid development of urbanization and industrialization, many green spaces have been replaced by urban buildings, resulting in decreased green spaces in courtyard space. Nonetheless, as an enclosed green space integrated with the natural environment, courtyard space plays a vital role in regulating environmental microclimate, so it is necessary to study its microclimate through vegetation greening. Therefore, this study took courtyard spaces in humid and hot areas as an example, and with the help of ENVI-met 5.5.1 software, introduced an orthogonal experimental design to simulate various plant layout models, including tree layout (TL), shrub layout (SL), grass layout (GL), and the interaction of their combined layout, and analyzed the simulation results of temperature, humidity, and wind speed. The results show that first of all, plant layout plays a crucial role in cooling and wind control, and the more uniform the plant layout, the better it is for cooling and ventilation. Secondly, plant layout showed a changing pattern of cooling and wetting in the morning, noon, and afternoon periods. Furthermore, TL had the best cooling and humidifying effect in the morning and midday, and the combined interaction of TL, SL, and GL and of SL and GL significantly affected the wind speed in the courtyard space. During the afternoon, the combined interaction of TL with SL and SL with GL outperformed the single-plant-element type of layout regarding cooling and humidification efficiency. Finally, scattered-form tree layout, single-form shrub layout, and 20% grass layout were the best combinations of plant layout for cooling, humidity reduction, and ventilation. The results provide reference data and an empirical case for the microclimate optimization of summer courtyard spaces.

Associations between maternal dietary intake and nutritional status with fetal growth at 14 to 26 weeks gestation: a cross- sectional study

BackgroundMaternal undernutrition during pregnancy is currently estimated at 23.5% in Africa, which is worrying given the negative impacts of malnutrition on maternal and fetal birth outcomes. The current study aimed at characterizing the associations of maternal dietary intake and nutritional status with fetal growth at 14–26 weeks gestation. It was hypothesized that maternal dietary intake was positively associated with maternal nutritional status and fetal growth both in early and late pregnancy.MethodsThis was a cross-sectional survey of 870 pregnant women in mid-western Uganda conducted in August 2013. Data were collected on women’s dietary intake (indicated by women’s dietary diversity and the diet quality score) and nutritional status (indicated by hemoglobin level and mid-upper arm circumference) at 14–26 weeks gestation. Fetal growth was determined by symphysis-fundal height Z-scores processed using the INTERGROWTH-21st calculator. Associations between maternal dietary intake and nutritional status with fetal growth were determined using correlations and chi-square tests.ResultsOverall, only 25% had adequate dietary diversity and the most utilized food groups were White tubers, roots and starchy vegetables; Pulses, nuts and seeds; Cereals and grains, Dark green leafy vegetables, and Fats and oils. A larger proportion of younger women (15–29 y) were classified as anemic (20.4% versus 4.4%) and underweight (23.7% versus 5.0%) compared to older women (30–43 y). Additionally, women aged 15 to 24 years had significantly lower mean SFH-for-gestation age Z-scores than women 36–43 years (F4, 783 = 3.129; p = 0.014). Consumption of legumes nuts and seeds was associated with reduced risk of anemia while consumption of dairy products (mostly milk) was positively associated with better fetal growth. Surprisingly, low Hb level was positively associated with normal fetal growth (rP = -0.133; p = 0.016) after 20 weeks gestation, possibly indicating normal fetal growth paralleled with physiologically necessary hemodilution.ConclusionsSub-optimal dietary patterns, characterized by limited dietary diversity and low protein intake, are likely to compromise maternal nutrition and fetal growth in limited resource settings. Improving pregnant women’s access to cheaper but nutrient-dense protein sources such as pulses, nuts and dairy products (mostly milk) has potential to improve women’s nutritional status and enhance fetal growth.

Lifestyle habits and examination findings before and during the COVID-19 pandemic: From health checkups in a rural area in Japan.

Aim: Few health checkup studies have reported lifestyle habits and examination results before and during the COVID-19 pandemic. We compared lifestyle habits and examination results surveyed before and during the COVID-19 pandemic. Methods: Five hundred and ten and 396 participants attended the health checkup in 2019 and 2022, respectively. The median age of females was 65 in 2019 and 2022, and that of males was 68 in 2019 and 69 in 2022. We investigated dietary and exercise habits, alcohol consumption, sleeping situation, and examination results, including body mass index (BMI), smell function, and blood examination results before and during the COVID-19 pandemic in a rural area in Japan. Results: An ordinal logistic regression analysis revealed that the intake frequency of vegetables and marine products was significantly less in 2022 than in 2019 after adjusting age and sex; green leafy vegetables (p = .016), fish (p = .002), and other marine products, including squid, shrimp, crabs, and octopus (p = .008). Alcohol consumption amount increased significantly in 2022 than in 2019 in men who drank beer (p = .007) and chuhai (p = .040). Albumin, hematocrit, cholesterols, and uric acid decreased, but serum calcium increased significantly in 2022 than in 2019 after adjusting age and sex. BMI and hemoglobin A1c were not significantly different between 2019 and 2022. A decrease in subjective smell feeling and smell test results was associated with decreased intake frequency of vegetables. Conclusion: Both dietary habits and blood examination results changed significantly during the COVID-19 pandemic in a rural area in Japan.

Design and Application of Bionic Camouflage Materials Simulating Spectral Reflection Characteristics of Plants: A Review

Hyperspectral remote sensing (RS) has rapidly developed in recent years and has been widely used in the military field. This technology not only brings huge opportunities for military reconnaissance but also poses unprecedented challenges to military camouflage, severely complicating the development of plant hyperspectral camouflage materials and technology. In this review, the spectral reflectance characteristics of plants and the application of hyperspectral RS in plant RS and military operations are reviewed. The development status of bionic camouflage materials that simulate the spectral reflection characteristics of plants is analyzed. With the existing hyperspectral camouflage materials and technology, bionic camouflage technology is limited by the inability of bionic materials to accurately imitate the characteristic absorption peaks of green vegetation, low stability and durability, and the large overall material thickness, which complicate actual large-scale application. On this basis, a future development direction and a trend of plant hyperspectral bionic camouflage materials and technology are proposed.