Damage Spots Research Articles

Drone photogrammetry as a tool for modern conflict archaeology: a case study of a Second World War armament bunker in Bavaria

ABSTRACT Within this paper, a case study on the application of drone photogrammetry at the Second World War armament bunker Weingut I is presented. The aim of the project was the mapping of the preserved remains in advance to the installation of a memorial site. In particular, the preserved concrete arch and one of the ventilation shafts were mapped by this method in high quality. Therefore, even small details of the building structure as well as several damage spots needing remediation became visible. One important advantage of drone prospecting is an enhanced occupational safety and a cost and time reduction as no further manual resources are necessary. In areas without finalized clearing for Unexploded Ordnance, other manual survey method would be especially dangerous. By comparing the photogrammetric model of the bunker Weingut I with corresponding ALS Digital Surface Models (DSM) of varying resolution, the increase in information is shown.

Evaluating a Novel Approach to Detect the Vertical Structure of Insect Damage in Trees Using Multispectral and Three-Dimensional Data from Drone Imagery in the Northern Rocky Mountains, USA

Remote sensing is a well-established tool for detecting forest disturbances. The increased availability of uncrewed aerial systems (drones) and advances in computer algorithms have prompted numerous studies of forest insects using drones. To date, most studies have used height information from three-dimensional (3D) point clouds to segment individual trees and two-dimensional multispectral images to identify tree damage. Here, we describe a novel approach to classifying the multispectral reflectances assigned to the 3D point cloud into damaged and healthy classes, retaining the height information for the assessment of the vertical distribution of damage within a tree. Drone images were acquired in a 27-ha study area in the Northern Rocky Mountains that experienced recent damage from insects and then processed to produce a point cloud. Using the multispectral data assigned to the points on the point cloud (based on depth maps from individual multispectral images), a random forest (RF) classification model was developed, which had an overall accuracy (OA) of 98.6%, and when applied across the study area, it classified 77.0% of the points with probabilities greater than 75.0%. Based on the classified points and segmented trees, we developed and evaluated algorithms to separate healthy from damaged trees. For damaged trees, we identified the damage severity of each tree based on the percentages of red and gray points and identified top-kill based on the length of continuous damage from the treetop. Healthy and damaged trees were separated with a high accuracy (OA: 93.5%). The remaining damaged trees were separated into different damage severities with moderate accuracy (OA: 70.1%), consistent with the accuracies reported in similar studies. A subsequent algorithm identified top-kill on damaged trees with a high accuracy (OA: 91.8%). The damage severity algorithm classified most trees in the study area as healthy (78.3%), and most of the damaged trees in the study area exhibited some amount of top-kill (78.9%). Aggregating tree-level damage metrics to 30 m grid cells revealed several hot spots of damage and severe top-kill across the study area, illustrating the potential of this methodology to integrate with data products from space-based remote sensing platforms such as Landsat. Our results demonstrate the utility of drone-collected data for monitoring the vertical structure of tree damage from forest insects and diseases.

A probabilistic estimation model for seismic physical portfolio loss of a water supply pipeline system

Losses due to hazards are inevitable and numerical simulations for estimations are complex. This study proposes a model for estimating correlated seismic damages and losses of a water supply pipeline system as an alternative for numerical simulations. The common approach in other research shows average damage spots per mesh estimated statistically independent to one another. Spatially distributed lifeline systems, such as water supply pipelines, are interconnected, and seismic spatial variability affects the damages across the region; thus, spatial correlation of damage spots is an important factor in target areas for portfolio loss estimation. Generally, simulations are used to estimate possible losses; however, these assume each damage behaves independently and uncorrelated. This paper assumed that damages per mesh behave in a Poisson distribution to avoid over-dispersion and eliminate negative losses in estimations. The purpose of this study is to obtain a probabilistic portfolio loss model of an extensive water supply area. The proposed model was compared to the numerical simulation data with the correlated Poisson distribution. The application of the Normal To Anything (NORTA) obtained correlations for Poisson Distributions. The proposed probabilistic portfolio loss model, based on the generalized linear model and central limit theory, estimated the possible losses, such as the Probable Maximum Loss (PML, 90% non-exceedance) or Normal Expected Loss (NEL, 50 % non-exceedance). The proposed model can be used in other lifeline systems as well, though additional investigation is needed for confirmation. From the estimations, a seismic physical portfolio loss for the water supply system was presented. The portfolio was made to show possible outcomes for the system. The proposed method was tested and analyzed using an artificial field and a location-based scenario of a water supply pipeline system. This would aid in pre-disaster planning and would require only a few steps and time.

Hot spots of radiation damage from extensive water ionization around metal ions.

Hot spots of radiation damage from extensive water ionization around metal ions.

Regulation of XPC Binding Dynamics and Global Nucleotide Excision Repair by p63 and Vitamin D Receptor.

p63 and the vitamin D receptor (VDR) play important roles in epidermal development and differentiation, but their roles and relationship in the response to ultraviolet (UV) radiation are less clear. Using TERT-immortalized human keratinocytes expressing shRNA targeting p63 in concert with exogenously applied siRNA targeting VDR, we assessed p63 and VDR's separate and combined effect on nucleotide excision repair (NER) of UV-induced 6-4 photoproducts (6-4PP). Knockdown of p63 reduced VDR and XPC expression relative to nontargeting controls, while knockdown of VDR had no effect on p63 and XPC protein expression, though alone it modestly reduced XPC mRNA. Upon UV irradiation through filters with 3 μm pores to create spatially discrete spots of DNA damage, keratinocytes depleted of p63 or VDR exhibited slower removal of 6-4PP than control cells over the first 30 min. Costaining of control cells with antibodies to XPC revealed that XPC accumulated at DNA damage foci, peaking within 15 min and gradually fading over 90 min as NER proceeded. In either p63- or VDR-depleted keratinocytes, XPC overaccumulated at spots of DNA damage so that 50% more XPC was retained at 15 min and 100% more XPC was retained at 30 min than in control cells, suggesting dissociation of XPC after binding was also delayed. Concurrent knockdown of VDR and p63 resulted in similar impairment of 6-4PP repair and XPC overaccumulation but even slower release of XPC from DNA damage sites such that 200% more XPC was retained relative to controls at 30 min post-UV. These results suggest that VDR accounts for some of p63's effects in delaying 6-4PP repair associated with overaccumulation and slower dissociation of XPC, though p63's regulation of basal XPC expression appears to be VDR-independent. The results are consistent with a model where XPC dissociation is an important step during NER and that failure to do so may inhibit subsequent repair steps. This work further links two important regulators of epidermal growth and differentiation to the DNA repair response to UV.

High average power optical properties of silica aerogel thin film

Silica aerogels synthesized by different techniques have been studied for their electrical and thermal insulating properties, mostly in their bulk structures; however, the optical properties of a silica aerogel thin film have remained largely unexplored. Due to their high porosities, silica aerogel thin films may be useful as very low-index optical materials, especially in multilayer coatings for high intensity and high-power lasers, where the large bandgap of silica is particularly valuable. In this paper, silica aerogel thin films were fabricated by spin-coating a silica sol, derived from a two-step acid/base catalyzed technique at an ambient pressure, on fused silica substrates. The films showed very low refractive indices (n) around 1.1 (approximately 72% porosity) and low absorptions between about 6 and 16ppm, lower than plasma-enhanced chemical vapor deposition (PECVD) comparison films. Optical scatterings of the silica aerogel films were measured and found to be comparable to a PECVD silica film and a bare fused silica substrate, with most films showing slightly higher scattering but one film showing lower. The laser-induced damage thresholds (LIDT) of all films were measured using carbon particle contamination, which allows testing over statistically large areas using continuous wave (CW) laser illumination. The LIDT of silica aerogels was similar to that of pure high-density silica and much higher than that of other common high-LIDT films such as hafnia and alumina. Most damage spots on silica aerogel samples only showed slight discoloration at irradiance levels of 150 kW/cm2 (1.5 × 109 W/m2), while similar tantala high reflectivity coatings failed catastrophically at 75 or 86 kW/cm2 (7.5 × 108 or 8.6 × 108 W/m2). Moreover, aerogels performed somewhat better than PECVD silica, where most damage occurred at a lower irradiance level of 100 kW/cm2 (1 × 109 W/m2) with a larger discolored spot.

Comparative study between effects of ginkgo biloba extract and extract loaded on gold nanoparticles on hepatotoxicity induced by potassium bromate.

In human organs, potassium bromate (KBrO3) produces toxicity. The main causes of KBrO3 hepatotoxicity are the formation of reactive oxygen species (ROS) and DNA damage. The purpose of this study is to show how ginkgo biloba extract (GBE) and extract loaded with nanogold particles (GBE@AuNPs) affect hepatotoxicity caused by KBrO3. The rats were separated into eight groups: control (group I), GBE (group II), AuNPs (group III), GBE@AuNPs (group IV), KBrO3 (group V), KBrO3 and GBE (group VI), KBrO3 and AuNPS (group VII), and KBrO3 and GBE@AuNPs (group VIII). KBrO3 generated DNA damage spots in a comet assay, which were associated with increased inflammatory indicators (IL-6), decreased anti-apoptotic Bcl-2, and increased apoptotic markers (Bax and caspase-3). The inflammatory, apoptotic, and ultrastructural alterations in liver tissue produced by KBrO3 were reduced in treated groups VI, VII, or VIII. The hepatotoxic effects of KBrO3 were reduced when GBE, AuNPs, or GBE@AuNPs were used; the particular GBE@AuNPs were the most effective.

Performance Evaluation of Cauliflower Cultivars in Mid-Hills of Nepal for Winter Season Production

Cauliflower (Brassica Oleracea botrytis) is one of the most popular and demanded vegetables in Nepal. Five cultivars: Barkha, Girija, Giewont, Whistler and Snow Mystique were transplanted in the first week of October 2016 and 2017 in the mid-hills (Kathmandu valley, 1300 masl) and grown during the winter season with 60X45 cm spacing. Source seed of Snow Mystique was Takii Seed Japan and the rest were Montsanto Seed, India. Crops were fertilized with 200:120:80N.P.K Kg/ha and 15-ton FYM/ha. Insecticide and fungicide were sprayed one time in the early period for crop establishment. The main objective of this experiment was to find out suitable high yielding and insect pest and disease tolerant hybrid cauliflower cultivars for commercial farming in the mid-hills of Nepal. Crops were evaluated with their vegetative, insect pest and disease, yield and farmers' and consumers' response in two consecutive years. Results showed that among the tested cultivars, Barkha was found to be highly uniform, vigorous, less attacked by insect pests and disease, early harvestable (62.7 days after transplanting), efficient per day yield (487.7 kg/ha), preferable average head weight (1039 g), freshness (4.3) and market preference (4.0) as compared to check variety Snow Mystique. On the other hand, Snow Mystique has only yield efficiency (436.2 kg/ha/day), greater insect damage and leaf spot disease, longer days to harvest (101 days), and freshness (3.8), respectively. Hence, Barkha has been selected and recommended for commercial cultivation in the mid-hills of Nepal.

Transverse and axial resolution of femtosecond laser ablation.

Femtosecond lasers are capable of precise ablation that produces surgical dissections in vivo. The transverse and axial resolutions of the laser damage inside the bulk are important parameters of ablation. The transverse resolution is routinely quantified; but the axial resolution is more difficult to measure and is less commonly performed. Using a 1040-nm, 400-fs pulsed laser, and a 1.4-NA objective, we performed ablation inside agarose and glass, producing clear, and persistent damage spots. Near the ablation threshold of both media, we found that the axial resolution is similar to the transverse resolution. We also ablated neuron cell bodies and fibers in Caenorhabditis elegans and demonstrate submicrometer resolution in both the transverse and axial directions, consistent with our results in agarose and glass. Using simple yet rigorous methods, we define the resolution of laser ablation in transparent media along all directions.

Comprehensive ablation study of near-IR femtosecond laser action on the titanium-based alloy Ti6Al4V: morphological effects and surface structures at low and high fluences

The surface of a titanium-based alloy Ti6Al4V was subjected to modifications by a near-IR femtosecond Ti:Sapphire laser, emitting at 775 nm pulses of 200 fs duration, in single-pulse and multi-pulse regimes, with up to 400 accumulated pulses, and pulse energies ranging from 2.5 to 250 upmu J. The whole range of induced effects is presented, from gentle ablation and pattern occurrence to substantial crater formation. Very observable laser-induced parallel periodic surface structures are reported, appearing both within the damage spot area, with low fluences, and at the peripheries of the craters, with higher fluences—but also on crater walls, and inside the crater structures. Damage threshold fluences ({F}_{mathrm{th}}) and the incubation factor (zeta ) were also determined.Graphic abstract

Image Analysis Reveals That Lenticel Damage Does Not Result in Black Spot Development but Enhances Dehydration in Persea americana Mill. cv. Hass during Prolonged Storage

Black spot corresponds to a physiological disorder of the type of oxidative stress that occurs after the prolonged postharvest storage of Persea americana Mill. cv. Hass fruit. Industry tends to confuse this disorder with pathogen attack (Colletotrichum gloeosporioides), chilling injury, mechanical damage during harvest and transport or lenticel damage. The main objectives of this research were: (i) to develop a method to assess and differentiate lenticel damage and black spot and (ii) to study the correlation between mechanical damage and lenticel damage on the development of black spot. Avocado fruits from different orchards were evaluated at two sampling times using different harvesting systems (conventional and appropriate) and at two times of the day (a.m. or p.m.). Here, we report a method based on image analysis to differentiate and quantify lenticel damage and black spot disorder. In addition, the results show that conventional harvest increased lenticel damage and lenticel damage did not correlate with black spot development but correlated with increased weight loss during prolonged postharvest storage. These results have important commercial implications since the appropriate harvesting of avocado cv. Hass would not only control the incidence of lenticel damage, which would be an advantage in terms of external quality, but also reduce weight loss during transport to distant markets.

515 p63 regulates XPC binding dynamics and global nucleotide excision repair in keratinocytes

The p63 transcription factor, critical for epidermal development, also regulates the response to DNA damage, including global nucleotide excision repair (NER) efficiency. We have reported that vitamin D receptor (VDR) is also required for efficient global NER of ultraviolet radiation (UV)-induced 6-4 pyrimidine-pyrimidone photoproducts and for releasing XPC—the initial ultraviolet radiation (UV)-induced DNA damage recognition sensor—from DNA damage. Since p63 and VDR have been reported to regulate each other, we assessed p63’s effect on XPC binding and dissociation. TERT-immortalized keratinocytes expressing shRNA targeting all isoforms of p63 exhibited reduced levels of VDR relative to non-targeting controls. Upon UV irradiation through 3 μm pores in otherwise opaque filters to create sub-nuclear focal spots of DNA damage, keratinocytes depleted of p63 exhibited slower removal of 6-4 photoproducts than control cells over 90 minutes. Co-staining with antibodies to XPC revealed that XPC rapidly accumulated at DNA damage foci, peaking within 15 minutes and gradually fading over 90 minutes as NER proceeded in control keratinocytes. In p63-depleted keratinocytes, XPC associated with DNA damage with comparable efficiency, but the dissociation of XPC was delayed so that substantially more XPC was retained at 30 minutes than in control cells. These results support a model where XPC dissociation is necessary for normal completion of subsequent NER steps and confirm our previous observations that p63 is important for efficient removal of UV-induced DNA damage. The results recapitulate those in keratinocytes depleted of VDR and are consistent with crosstalk between p63 and VDR in promoting normal dissociation of XPC from damaged DNA such that absence of either p63 or VDR can lead to non-productive binding of XPC and reduced NER. These results further link two important regulators of epidermal growth and differentiation to DNA repair.

Assessment of Machine Learning Algorithms for Modeling the Spatial Distribution of Bark Beetle Infestation

Machine learning algorithms (MLAs) are used to solve complex non-linear and high-dimensional problems. The objective of this study was to identify the MLA that generates an accurate spatial distribution model of bark beetle (Ips typographus L.) infestation spots. We first evaluated the performance of 2 linear (logistic regression, linear discriminant analysis), 4 non-linear (quadratic discriminant analysis, k-nearest neighbors classifier, Gaussian naive Bayes, support vector classification), and 4 decision trees-based MLAs (decision tree classifier, random forest classifier, extra trees classifier, gradient boosting classifier) for the study area (the Horní Planá region, Czech Republic) for the period 2003–2012. Each MLA was trained and tested on all subsets of the 8 explanatory variables (distance to forest damage spots from previous year, distance to spruce forest edge, potential global solar radiation, normalized difference vegetation index, spruce forest age, percentage of spruce, volume of spruce wood per hectare, stocking). The mean phi coefficient of the model generated by extra trees classifier (ETC) MLA with five explanatory variables for the period was significantly greater than that of most forest damage models generated by the other MLAs. The mean true positive rate of the best ETC-based model was 80.4%, and the mean true negative rate was 80.0%. The spatio-temporal simulations of bark beetle-infested forests based on MLAs and GIS tools will facilitate the development and testing of novel forest management strategies for preventing forest damage in general and bark beetle outbreaks in particular.

Vitamin D Receptor Promotes Global Nucleotide Excision Repair by Facilitating XPC Dissociation from Damaged DNA

Vitamin D receptor (VDR) is important for normal DNA repair, although the mechanism by which it acts is unclear. After focal UV irradiation to create subnuclear spots of DNA damage, epidermal keratinocytes from VDR-null mice as well as human epidermal keratinocytes depleted of VDR with small interfering RNA removed pyrimidine-pyrimidone (6-4) photoproducts more slowly than control cells. Costaining with antibodies to XPC, the DNA damage recognition sensor that initiates nucleotide excision repair, showed that XPC rapidly accumulated at spots of damage and gradually faded in control human keratinocytes. In VDR-depleted keratinocytes, XPC associated with DNA damage with comparable efficiency; however, XPC's dissociation dynamics were altered so that significantly more XPC was bound and retained over time than in control cells. The XPF endonuclease, which acts subsequently in nucleotide excision repair, bound and dissociated with comparable kinetics in control and VDR-depleted cells, but the extent of binding was reduced in the latter. These results as well as kinetic modeling of the data suggest that VDR's importance in the repair of UV-induced DNA damage is mediated in part by its ability to facilitate the dissociation of XPC from damaged DNA for the normal recruitment and assembly of other repair proteins to proceed.

Position-Dependent Effect of Guanine Base Damage and Mutations on Telomeric G-Quadruplex and Telomerase Extension.

Telomeres are hot spots for mutagenic oxidative and methylation base damage due to their high guanine content. We used single-molecule fluorescence resonance energy transfer detection and biochemical assays to determine how different positions and types of guanine damage and mutations alter telomeric G-quadruplex structure and telomerase activity. We compared 15 modifications, including 8-oxoguanine (8oxoG), O-6-methylguanine (O6mG), and all three possible point mutations (G to A, T, and C) at the 3' three terminal guanine positions of a telomeric G-quadruplex, which is the critical access point for telomerase. We found that G-quadruplex structural instability was induced in the order C < T < A ≤ 8oxoG < O6mG, with the perturbation caused by O6mG far exceeding the perturbation caused by other base alterations. For all base modifications, the central G position was the most destabilizing among the three terminal guanines. While the structural disruption by 8oxoG and O6mG led to concomitant increases in telomerase binding and extension activity, the structural perturbation by point mutations (A, T, and C) did not, due to disrupted annealing between the telomeric overhang and the telomerase RNA template. Repositioning the same mutations away from the terminal guanines caused both G-quadruplex structural instability and elevated telomerase activity. Our findings demonstrate how a single-base modification drives structural alterations and telomere lengthening in a position-dependent manner. Furthermore, our results suggest a long-term and inheritable effect of telomeric DNA damage that can lead to telomere lengthening, which potentially contributes to oncogenesis.

643 XPC dissociation from damaged DNA and efficient global nucleotide excision repair depend on vitamin D receptor

Vitamin D and its receptor, VDR, together and independently, have been associated with DNA repair, though the mechanism by which they act is unclear. Upon ultraviolet irradiation through 3 mm pores in otherwise opaque filters to create focal spots of DNA damage, epidermal keratinocytes from both VDR-null mice and human keratinocytes depleted of VDR with siRNA exhibited slower removal of 6-4 photoproducts than normal control cells over 90 minutes. Co-staining with antibodies to XPC, the initial UV-induced DNA damage recognition sensor, revealed that XPC rapidly accumulated at DNA damage foci and gradually faded over 90 minutes as nucleotide excision repair proceeded in control human keratinocytes.

Development of a defect recognition algorithm for visual laser-induced damage detection

Laser-induced damage is defined as a permanent detrimental change in the characteristics of an optical element caused by a laser beam. This change can be observed by many different inspection techniques, of which optical and phase imaging microscopic techniques have superior sensitivity. However, such examinations conducted by human operators are relatively slow and subjective—so they cannot be used for online damage monitoring purposes, whereas automatic inspection systems have advantages in terms of sensitivity, reliability, and speed. In this paper we introduce a new method for the computer-aided recognition of damaged sites based on visual images taken from the sample surface by a CCD camera. The evaluation procedure is performed by a computer algorithm, which consists of exact, statistically established steps. It includes noise reduction by considering the statistical behavior of photon noise. Besides, it takes into account the spatial extent of a damage spot by nonlinear image filtering to separate damage-indicating intensity changes from random noise. This mimics the ability of the human eye to distinguish features from their surroundings. The evaluation algorithm is built of computationally less demanding mathematical operations to enable fast execution which is vital for monitoring at high repetition rates. The proposed method was tested on a sizeable dataset of images yielding 98.8% of damage detection efficiency. It was also compared to a generally used visual laser damage detection procedure, which has a success rate of 88.6%. This yields one order of magnitude reduction in the number of undetected damaged sites.

Field cage assessment of feeding damage by Halyomorpha halys on kiwifruit orchards in China

The brown marmorated stink bug (BMSB), Halyomorpha halys (Stal) (Hemiptera: Pentatomidae), is a polyphagous pest of fruit and vegetable crops. Kiwifruit is an economically important fruit, and occasional outbreaks of BMSB have been reported in kiwifruit orchards in China, Italy, and Greece. However, information on the types of damage and damage levels on fruit that BMSB can cause during the growing season are currently lacking. To address this, we conducted field experiments using exclusion cages to investigate feeding damage caused by BMSB to three kiwifruit cultivars [Actinidia chinensis var. deliciosa ‘Hayward’ (green), A. chinensis var. chinensis ‘Nongdajinmi’ (yellow), and A. chinensis var. deliciosa ‘Xuxiang’ (green)]. We also investigated damage levels at different insect and kiwifruit densities on ‘Hayward’. Results indicate both nymphs and adults caused green and white damage spots under the skin of kiwifruit. Green and white spots were found mixed or singly in all three cultivars. The proportion of each type of damage spot varied with the fruit development stage and the cultivar. The incidence of fruit damage was not significantly different among the cultivars (‘Hayward’ 76.9%, ‘Xuxiang’ 82.5%, ‘Nongdajinmi’ 73.5%); however, lower intensity of fruit damage was observed on ‘Nongdajinmi’. The incidence of fruit damage in ‘Hayward’ did not differ significantly among different insect/fruit densities, while intensity of fruit damage increased with increasing ratio of insects to fruit. The intensity of fruit damage significantly increased when four or more insects were introduced to cages when compared with two. These results provide key information on the characteristics of BMSB damage in kiwifruit.

Filament-wound composite pressure vessel inspection based on rotational through-transmission laser ultrasonic propagation imaging

In this paper, we introduce a rotational through-transmission ultrasonic propagation imaging system to inspect the full thickness of the entire cylindrical section of filament-wound composite overwrapped pressure vessels. This system can achieve high signal-to-noise ratio throughout the cylindrical section of pressure vessels by using a rotational scanning method. Based on the advantages of the laser ultrasonic system that can generate and detect ultrasound waves by using a small-diameter laser beam and making an inspection path in a narrow environment, this system directs the laser beam inside the pressure vessel via a mirror to enable the through-transmission mode for vessel inspection. A type-3 pressure vessel was inspected using the system developed, which successfully detected two impact damage spots and showed the filament winding direction clearly. The test results show that the system developed is highly reliable and can replace the current hydrostatic sample test for pressure vessel safety certification.

Measurements of heat flow from surface defects in lithium triborate.

We present what is, to our knowledge, the first measurement of temperature distributions in a nonlinear optic resulting from absorption in a localized surface defect. These measurements were performed on principal cut samples of lithium triborate with damage spots centered on their front surfaces, pumped by a kW-scale continuous-wave laser. The changes in optical-path length associated with this heating were measured with a Mach-Zehnder interferometer, from which the temperature distribution could be inferred. These distributions have sharper features with larger magnitudes than would be expected with bulk-absorption heating. Comparison with both numerical and analytical models is used to qualify the measurements and to estimate the total power absorbed at a given site using this bulk material response. While sensitivity is dependent on the properties of the material of study, we demonstrate measurements of absorption levels of one part in 105.