Dairy Units Research Articles

Estrategia colectiva de pequeñas unidades económicas lecheras que operan en un mercado oligopsónico

This article addresses a problem in the Mexican dairy industry and involves stake-holders upon whom the operation of this sector depends. I am referring to the small dairy units, which have long been in a vulnerable situation due to their operating in an oligopsonistic market where the processors hold dominance. A lack of technology, resources, training, business knowledge, project entrepreneurship, along with isolat-ed operation, exacerbates the situation. Since the late 20th century, cooperation has emerged as a strategy for small and medium-sized economic units to survive in a chal-lenging market and to improve their well-being. The work aims to highlight a model of business cooperation between dairy units. By pooling resources and efforts, produc-tive development is strengthened with the goal of achieving survival and in order to contribute to regional development. The case study of Cooperative Prolea in Acatic, Jalisco is used

Training needs of tribal youth and constraints faced by tribal youth in choosing agricultural enterprises

Youth is that vibrant time in a person’s life when they can channel their vigour and energy into influencing the course of an entire country. The Ministry of Human Resource Development (1985) considers “youth group” in India as the person under the age group of 15 to 35 years. Tribal youth refer to the youth community belonging to the age group of 15-35 years and have commonality in terms of name, culture, dialect, territory and taboos. Identifying the training needs is the first step of any training programme for a specific target group. The training for tribal youth could serve as an effective tool for developing positive attitude with improved confidence and greater willingness towards agricultural enterprises. This further could help in development of training modules in various agricultural enterprises. It was observed that the overall Training Need Index (TNI) of the study area was 0.48, which was under medium training needs. Within that paddy crop production had 0.46 and Dairy farming had 0.51 index values. It was also observed that only one training need area had high level of training need i.e., selection of variety (0.63) and very high training need level i.e., Health management of dairy animal (0.81). The important constraints reported by the tribal youth were lack of participation in Panchayat Raj System, absence of dairy unit, problems in identification of animal diseases, inadequate irrigation facilities, low rate of milk, etc.

Integrated Farming System for a Sustainable Livelihood

This paper discusses about the success story of farmer practicing Integrated Farming System (IFS) for sustainable livelihood. Present study was conducted during 2018-19 in Bayyaram village of Bayyaram mandal in Khammam district of Telangana State. A case study method was followed to document the success of IFS farmer. Mr. Vidyasagar hails from Bayyaram village of Bayyaram mandal in Khammam district of Telangana State. He adopted different IFS components such as Horticulture, Dairy, Poultry, Piggery and Fishery units. He closely supervised multiple enterprise units and followed new technologies in IFS to get sustainable livelihood. He used new methods of cultivation in IFS and believed that diversification with various components of farming systems results in desired profitability for IFS farmers. Hence, integration of different components with higher input recycling increased farm productivity of different farm components and also he was able to provide employment opportunities to other farmers through IFS.

Emergence and Distribution of Diverse Serovars of Non-Typhoidal Salmonellae in Mangalore, Coastal Karnataka: A 12-year Experience

Background and Aim: Non-Typhoidal Salmonellae (NTS) is emerging as a major health problem globally. They cause foodborne infections, especially gastroenteritis as well as extraintestinal manifestations. As this is a zoonotic disease, certain animal sources such as poultry, egg, meat and dairy products are implicated. Fruits and vegetables too contaminated by manure of infected animal excreta are possible vehicles of transmission of this disease. The present report analyzes the spectrum of NTS with seroprofiling, isolated in our institute for the last 12 years. Materials and Methods: Fresh fecal samples from patients with acute gastroenteritis and blood samples from cases of bacteremia were processed according to the standard protocol. Nonlactose fermenting colonies resembling Salmonella were subjected to biochemical characterization and antimicrobial susceptibility testing followed by serotyping. Results: One hundred fifty-two NTS strains were isolated from feces and 13 from blood. Serotyping of these strains revealed that the most common NTS species was Salmonella Typhimurium (44.8%), followed by Salmonella Oslo (15.2%) and Salmonella Weltevreden (11.5%). Many rare strains were also encountered in our study. Conclusion: As many of the NTS species are biochemically similar, serotyping of NTS should be made mandatory to know the burden of the disease and to detect the emergence of new serovars. Emergence of multidrug resistance in NTS strains is a major concern nowadays. It is very essential to adopt strict quality measures in poultry, fish, dairy, and meat processing units as they are widely distributed in animals. In addition, strategies should be formulated by health education and public awareness programs to sensitize the population regarding this menace.

Effect of Phosphorus and Zinc on Fodder Yield and Quality of Leguminous Fodder: Berseem (Trifolium alexandrinum L.)

The contribution of the livestock sector to the Indian Economy is 4.11% of its total GDP and it is nearly 25.6% for total agricultural GDP. Small and marginal farmers are mainly attached to the mixed farming of field crops and dairy animals. Farmers get regular income from their dairy unit and also use dairy animals as economic security. Though India comes under the highest livestock populated countries, the production of milk is lower than expected because of improper feeding quality. Through the last two decades, the demand and price of milk both increase gradually, and farmers are not getting that much profit because of their dependency on ready cattle food. The gross profit after the selling of milk is nearly equal to the feeding expenditure. For the use of paddy straw as dry fodder, the quality and quantity of milk production are decreased. So, green fodder is the best option for livestock as it contains adequate nutrients as well as crude fibre. Among the fodder and forage crops, berseem is the second maximum cultivated fodder crop which possesses a 2 million ha area of fodder crops in India. There are several advantages of berseem as a fodder crop such as a short-duration crop, multi-cut nature, the capability of higher biomass production, the presence of a good amount of crude protein, cellulose, total digestible nutrients and succulent nature. Though Phosphorus and zinc play an antagonistic effect on each other, both are required for the growth and development of berseem crops. From cellular respiration to enzymatic reactions, phosphorus is essential for completing several metabolic activities. Zinc is related to yield and fodder quality by completing plant metabolisms like carbohydrates and auxin production. Adequate management practice is required to maintain the concentration of both nutrients at an optimum level. In Indian soil, zinc is deficient and phosphorus is mostly present in organic form. So, the use of biofertilizers will be effective to convert those complex nutrients form into simple and available for the plant.

Učinkovitost sustava automatske detekcije tjeranja u junica smeđeg goveda kada se rabi seksirano sjeme na velikoj farmi mliječnih goveda

The identification of cows and heifers in heat and their timely artificial insemination (AI) is an important issue for large dairy units. The objective of the research was to study the efficiency of insemination of oestrous Brown Swiss heifers using am automated heat detection system (AHD) compared to heat detection by visual observation (VO). The AHD system application increased the fertility of heifers by 8.9% when using conventional semen (unsexed) and by 14.4% using sexed semen. The number of services per conception when using sexed semen decreased from 3.7±1.08 with VO to 2.4±0.68 with AHD. Two-factor analysis of variance showed that an 89% effect of managerial decisions such as the detection of heifers in heat by means of AHD and the use of sexed semen on the efficiency of insemination. This study showed that professional breeders and veterinarians should pay particular attention to AI management when making these managerial decisions in large dairy units.

Softened Water and Steam Boiler Water Condensate Quality used in the Milk Production Line of a Private Dairy Unit Located in Bechar Province (South-West of Algeria): Physicochemical and Bacteriological Analysis

Background: This study aimed at assessing the physicochemical and bacteriological parameters of the water at the softener outlet and the steam boiler water condensate of a dairy unit located in Bechar province (South-West of Algeria). Methods: Some physicochemical and bacteriological parameters were analyzed based on standard methods given by the American Public Health Association (APHA). Result: The obtained results showed that the parameters: pH, EC, salinity, TA, TAC, sulfate, nitrate, and fluoride ions of most analyzed water samples at the softener outlet (SW) were in agreement with national regulations. However, the steam boiler water condensate samples (BW) were out of specification except for TA, TAC, nitrate, and fluoride ions. The SW and BW samples were qualified as very hard exceeding the standard set at 15°f and 10,5 to 11,5°f, respectively, with high salinity and TDS values. The bacteriological analysis showed that most of the steam boiler water condensate samples complied with national regulations with the absence of fecal coliforms, knowing that the BW5 and BW8 samples were contaminated with spores of sulfite-reducing Clostridia at a load of 3 and 1 spore/50 mL, respectively. However, an average of 6/10 (i.e. 60%) of the softened water samples were contaminated with the opportunistic species ‘Pseudomonas aeruginosa’, while the SW5, SW8 and SW10 samples were contaminated with spores of sulfite-reducing Clostridia (1 to 11 spores/50 mL). All samples were free from Salmonella spp. A high TH of the water at the softener outlet reveals a malfunction of the external treatment (softening). On the one hand, boiler scaling that may occur can reduce the steam flow (low efficiency of the heat treatment), which contributes to poor pasteurization of the milk, especially in the presence of bacterial contaminants, and on the other hand, increase the energy expenditure.

Trend and future perspective of milk production in Karnataka

The present study was conducted to understand the present and future perspective of milk production in Karnataka and to develop a suitable model for prediction of milk production in the state. The present study is having advantage as the past trends of milk production in the state have been impressive with growth, contributing significantly to overall milk production in the country. The Auto-Regressive Integrated Moving Average(ARIMA) and Artificial Neural Network (ANN) models were applied to the data sets of different dairy units for modeling and forecasting milk production in the state. The milk production of different dairy units has indicated a significant and positive trend over the years (Mann Kendal and Sen’s estimator). The purpose of the study was based on the accuracy criteria to identify the best ARIMA and ANN models for forecasting. It was found that the ARIMA model predicted cows, goats, and total milk production with better R-Square, MAPE and percentage prediction error compared to ANN models. ANN model performed better in predicting the buffalo’s milk yield compared to the ARIMA model. The best-identified models were used for out-sample forecast.

PSX-B-12 Effect of Locust Bean gum on Gene Expression in cow Blood

Abstract Locust bean gum (LBG) is a galactomannan extracted from the seeds for use as a food thickener and stabilizer and is claimed to have applications in the biopharmaceutical field. The possible immunomodulatory effects of LBG have been studied in cell lines. The objective of this study was to evaluate the effect of LBG on the expression of genes associated with innate and adaptive immune responses in cow blood. Blood was collected from (n=3) clinically healthy Holstein-Friesian cows from the North Carolina A&T State University Dairy Unit. Whole blood (1 mL) from three cows was treated individually with 10 ng/mL of LBG (Sigma-Aldrich St. Louis, MO) or maintained in PBS, incubated at 37ºC for 30 minutes. Total RNA was extracted, reverse transcribed, and real-time PCR was carried out using the RT2 Profiler human Innate & Adaptive Immune Responses PCR Array containing 84 genes, as recommended by the manufacturer (Qiagen). The Livak method was used to calculate fold change (FC >2 considered significant). The analysis showed that 40 genes out of 84 genes were affected by treatment with LBG. Among 40 genes, 4 were upregulated, and 17 genes were downregulated. The highest up-regulated and down-regulated genes following exposure to LBG were Metaxin 1(MX1) and Recombination activation gene 1 (RAG1) respectively. The MX1 gene, which encodes a protein located on the outer membrane of mitochondria, is a component of the mitochondrial protein translocation apparatus. The RAG-1 gene is involved in the activation of immunoglobulin V-D-J recombination and immune homeostasis. Thus, gums may have bioactive roles in cow blood. The observed differential effects seen have implications for the function of the molecules examined possible epigenetic regulation of V(D)J recombination and potential for dietary modulation of animal health using gums and dietary fiber and warrant further study.

Gyan Fresh: Digital Transformation of Dairy Business with Resilience and Technology Innovation

Gyan Dairy, headed by the Agarwal brothers, was a privately held subsidiary of C. P. Milk, started as a small dairy unit in 2007. Gyan Dairy became one of the largest regional players in the state of Uttar Pradesh (India). The Indian government imposed the most strict and longest lockdown in 2019 to tackle the COVID-19 pandemic. This raised severe challenges for businesses, especially the dairy industry, which relies heavily on the movement of people and vehicles to ensure the delivery of products to consumers. Gyan dairy launched Gyan Fresh app to integrate the app-based home delivery business model for its dairy products. This case discusses the story of Gyan Fresh, which enabled self-orders and brought disruptive changes in the Indian dairy market and consumer behaviour. Furthermore, the case discusses the challenges and the next steps to expand the business, retain consumers and streamline the distribution channels.

Energy efficiency of integrated crop-livestock-fish farming system for lowland irrigated ecology of eastern region, India

An integrated farming system (IFS) model was developed and assessed at ICAR-Research Complex for Eastern Region, Patna during 2015–19. The aim of the study was to analyze energy input-output relationship of the IFS model. The IFS model comprised of field crops, vegetables, fruits, green fodder, cattle, fish, duck and vermicomposting components. The analysis revealed total energy input in the IFS model as 112.62 GGJ and total energy output as 211.12 GJ. Energy use efficiency of the IFS model was analyzed to be 1.87, net energy gain recorded as 98.5 GJ. The direct and indirect energy inputs were estimated to be 14.06 GJ and 98.56 GJ, respectively while renewable and non-renewable energy inputs were estimated to be 85.04 and 27.58 GJ, respectively. Further, the total energy input in dairy unit was estimated to be 70.3 GJ of which 68.3 GJ was provided through feed, which accounted for 97% of total energy input in dairy unit, and 60% of the total energy input in IFS model. Furthermore, energy input in the forms of labour, fossil fuel, electricity, fertilizers and machinery was required maximum in the field crops while water energy input was required maximum in fish pond. Results showed that individual farming of fish, duck and cattle is not viable in terms of energy use efficiency. However, the IFS model as a whole was found to be energy efficient and can be adopted in lowland irrigated ecologies of the eastern region of the country.

Glycerol as a partial replacement for lactose in milk replacer for young dairy calves

Glycerol (glycerin) is increasingly available from biodiesel manufacture and edible oil refining and it has been used successfully in diets for chickens, pigs, and adult cattle; however, less information is available on its nutritional value in young calves. Our objective was to determine the effects on calf growth and health when glycerol replaced a portion of lactose in milk replacer. Holstein calves (12 male, 12 female) born at the University of Illinois dairy unit were assigned alternately to 1 of 2 treatments (24 calves total): control milk replacer or milk replacer supplemented with 15% glycerol in replacement of lactose. The experimental base milk replacer contained greater protein, fat, minerals, and vitamins so that when glycerol was added, the composition would be the same as that of the control, except that glycerol replaced some lactose. Calves were housed in individual hutches bedded with straw, and water was freely available. Starter was offered beginning on d 36. The amount of milk replacer offered was reduced by half on d 43, and calves were weaned at d 49. Calves were fed milk replacers twice daily from d 3 of life. Milk replacers contained 28% protein (all from whey proteins), 2.6% lysine, and 15% fat. Control milk replacer contained 40% lactose, and the glycerol milk replacer contained 25% lactose. Both replacers were reconstituted to 15% solids. Glycerol (liquid) was added to reconstituted base milk replacer at each feeding. During wk 1, milk replacers were fed at a rate of 0.25 Mcal/kg of metabolic body weight (BW) (about 1.5% of BW daily as powder, or approximately 675 g/d) and from wk 2 to 6 at 0.30 Mcal/kg of metabolic BW (about 2% of BW daily, or approximately 900 to 1,200 g/d). Measurements of BW and stature were made weekly through d 56. Calf BW and average daily gain through d 35 (0.66 vs. 0.65 kg/d for controls and glycerol, respectively) did not differ significantly between treatments. Stature measurements (withers height, body length, heart girth) and measures of health (fecal scores, medical treatments) did not differ between treatments. Under the conditions of this experiment, glycerol was an acceptable replacement for at least 37.5% of the total lactose in milk replacer (15% of the formula) if economically favorable.

Traditional Livestock System and Improvement of Cow’s Hygienic Milk Quality Sold in N’Djamena, Chad

Unlike most Sahelian towns, the production and selling of milk in N’Djamena is generally made in a traditional way and causes a serious hygiene and public health problem. This survey aims to improve the hygienic quality of milk by reducing contamination at critical points in the milk production chain. The approach is based on using suitable, cleaning and disinfection equipment’s of milking, collection and storage. The results showed contamination had significantly decreased by comparing the situation before and after interventions. The average of total mesophilic flora felt from 3106 to 104, total coliforms from 6.8103 to 3.9101; thermotolerant coliforms from 2101 to 0.6101 and Staphylococcus from 1.07102 to 1.5101. The pH increased from 6.55 to 6.6 and the dornic acidity from 20 to 21. The investment cost is 15 FCFA/day in the village with 146 liters of milk sold at 275 FCFA per unit. It is 12 FCFA/day per dairy unit for 324.6 liters sold at 533.33 FCFA per unit. The model for improving the hygienic quality of milk sold in N’Djamena improved the physico-chemical qualities of milk and significantly reduced the rate of microbial contamination. The general hygiene improvement and especially personal hygiene quality was weak because it is very difficult to change traditional behavior, but the boil milk action contributed to reduce the initial contamination.

Factors Affecting Machinations at Dairy Farms : An Appraisal from Economic Perspective

This study was undertaken to get a comprehensive idea about the favourable and unfavourable factors for adoption of dairy automation/ machinations as perceived by dairy farmers, and study the economic impact of semi-automatic milking machine for small, medium and large dairy unit using the analysis of total cost and monetary benefits. The study was carried out in north Indian states, 30 commercial dairy farmers were selected constituting a total of 150 respondents practicing commercial dairy farming under survey method of investigation. Results revealed that time saving in dairy farm operations, drudgery reduction, and maintenance of hygiene and quality of milk and milk products were perceived as top three favourable factors for adoption of dairy automation or machination. The high initial investment required for dairy automation, no extra milk price for hygiene and quality products through the adoption of dairy automation in the market, and high cost of equipment and less subsidy provided by the government were perceived as top three favourable factors for adoption of dairy automation or machination. The study indicated that adoption score of the respondents were not significantly correlated with age. It implies that age, education, family size land holding and experiences of dairy farming does not affect the adoption of dairy automation technologies. The additional monetary benefits apart from this economic benefit’s other benefits such as clean and hygiene milk, health care and management in economic terms were calculated about 43800, 39,600, and 64,000 per year for small, medium, and large dairy unit, respectively

MOLECULAR CHARACTERIZATION OF ISOLATED LACTIC ACID BACTERIA FROM DIFFERENT TRADITIONAL DAIRY PRODUCTS OF TRIBES IN THE FARS PROVINCE, IRAN

Various traditional dairy products could be considered as an abundant source for Isolation/collection of new lactic acid bacteria (LAB) with unique characteristics. The present researchaimed to investigate the morphological, biochemical and genotypic characterization of dominant lactic acid bacteria that were isolated from traditional dairy products in Iranian tribes . A total of 75 samples of traditional yogurt, doogh from dairy units in Fars province were randomly collected. Isolation of lactic acid bacteria, biochemical and genotypic identification were conducted. Totally, 157 LAB isolates were selected. Cocci and rod shape LAB were 53.50% and 46.49%, respectively. Biochemical tests showed the occurrence of 40.6% of the strains were Streptococcus, 2.25% of the strains were enterococci and 20.3% of the strains were lactococci. Most isolated lactobacilli were related to Lactobacillus plantarum and Lactobacillus bulgaricus. Genetically the presence of the following species was verified: Lactococcus lactis subsp. cremoris, Lactobacillus plantarum, Streptococcus thermophilus, Lactobacillus fermentum, Lactococcus lactis subsp. lactis, Lactobacillus bulgaricus, Lactobacillus helveticus, and Lactobacillus casei. The current study showed that thestrains which were isolated from traditional dairy products were not only appropriatefor use as starter adjuncts or cultures, but also they may provide a valuable gene pool for research and production of commercial starters with specific traits.

Characteristics of kappa-casein gene polymorphism in cows of Swiss breed and their productive qualities at a large dairy

Kappa-casein is a fraction of the main milk protein, which determines the technological properties of milk and the quality of dairy products, especially cheese and fermented milk products. The study tested the distribution of kappa-casein gene genotype polymorphism (CSN3) in mothers and daughters of the Swiss breed cows, taking into account their milk productivity qualities. The study was performed on 111 cows of the Swiss breed on a large dairy. The observation group I included 51 cow-mothers of the Swiss breed; group II – 60 cow-daughters of the Swiss breed. In a study of the polymorphism of the kappa-casein gene in cow-mothers and cow-daughters of the Swiss breed, the dominance of allele B (0.73 and 0.70) was found, which was registered more than two times more often than allele A. The incidence of genotypes AA – AB – BB in cow-mothers of the Swiss breed was as follows: 5.9% – 43.1% – 51.0% with indicators of observed heterozygosity 0.43 and expected heterozygosity 0.40. In cow-daughters of the Swiss breed the frequency of the BB genotype was the same, but, genotype АА was registered almost twice as often, with lower indicators of observed heterozygosity and smaller increases of indicators of expected heterozygosity than in cow-mothers. Cow-mothers of the Swiss breed with the AA genotype, in comparison with the AB genotypes, had higher milk yields during lactation (by 43%), fat production (by 86.9%), ratio of fat to protein (by 40.5%). Also note, the cow-mothers of the Swiss breed with the AA genotype, in comparison with the BB genotypes, had reliably high rates of fat to protein (by 35.8%) and only a trend to higher fat production; fat and protein. The production of fat in dairy cows-daughters with the BB genotype was higher than with the AA genotype, but only at the level of tendency. The correlation analysis showed that the AA genotype of the kappa-casein gene in cow-mothers of the Swiss breed was associated with the duration of lactation, indicators of milk yield during lactation, fat production, fat content and protein in milk with registration of reliable and positive coefficients of correlation. The genotypes in cow-daughters did not have a reliable correlation with the duration of lactation and fat or protein content in milk. The results of the kappa-casein gene polymorphism study indicated that the AA genotype of cow-mothers of the Swiss breed was associated with higher productive milk qualities which should be taken into account when forming a highly productive herd of cattle of the Swiss breed under intensiveoperative technology at a large-scale dairy unit.

Effect of dairy unit operations on immunoglobulins, colour, rheology and microbiological characteristics of goat milk

Raw goat milk was processed by low-temperature long-time (LTLT) and high-temperature short-time pasteurisation, boiling, skimming, microfiltration, and concentration to 25% solids to investigate the effects on composition, rheology, immunoglobulins, lactoferrin and microbial quality. All processing interventions had significant effect on fat, protein and ash content. pH of goat milk was decreased by heating and concentration. Skimming had lesser reduction of immunoglobulin G (IgG), immunoglobulin A (IgA), and lactoferrin (LF), compared with those by microfiltration and heat treatments. Levels of IgG and lactoferrin were the lowest after LTLT pasteurisation, while IgA was the least after boiling. Skimming and microfiltration reduced L∗ values; heating increased b∗ values. Heating increased rennet gelation time and reduced coagulum strength. Microbiologically, heated and microfiltered goat milk were comparable, but the latter had reduced total bacteria and coliform counts. Heat stability maxima were at pH 6.8–7.0 for raw and heated goat milk; peak values were different.

Dairy entrepreneurship development in North East India: A case study of Friendship Dairy and Agro Product Unit of Manipur

India has been the leading producer and consumer of dairy products worldwide since 1998 with sustained growth in the availability of milk and milk products. Dairy activities form an essential part of the rural Indian economy, serving as a critical source of employment and income. The dairy market in India reached a value of INR 10,527 billion in 2019. As of 2020, approximately 4.2 per cent of India’s gross domestic product is due to dairy production. The present study was performed at Friendship Dairy and Agro Product Unit of Manipur to examine the product availability and performance and also to investigate the marketing strategies and analyze the marketing channels. Both primary and secondary data were collected for the study The major products of Friendship Dairy are pasteurized toned milk, pasteurized curd, ghee and paneer which are available in different packs and sizes. Highest net sales (Rs. 2,78,78,928.00) and profit (Rs. 5,04,442.00) of Friendship Dairy were recorded in 2018-19. Two prominent marketing channels were identified in the study and marketing channel comprising of Producer – Distributor – Retailer – Consumer was found to be dominant through which nearly 78 per cent of the milk and milk products were moved around the Imphal City. The firm has effective marketing strategies with respect to products, price and promotions, which may help them in running the enterprise successfully for a longer period of time.

Differential Expression of Cow Innate and Adaptive Responses Genes in Response to Eugenol

Abstract Dietary phytochemicals have both nutritional and health benefits for farm animals. Research on the immunomodulatory effects of phytochemicals may aid in developing novel therapeutic agents and provide insights into the regulation of gene expression. Eugenol (4-allyl-2-methoxyphenyl) is the active ingredient in clove oil that has been studied for its immunomodulatory/anti-inflammatory effects. The objective of this study was to evaluate the effect of eugenol on the expression of genes associated with the cow’s innate and adaptive immune responses. Blood was collected from (n = 3) clinically healthy Holstein-Friesian cows from the North Carolina A&T State University Dairy Unit. One milliliter of whole blood from three cows was treated individually with 10 ng/mL of Eugenol (Sigma-Aldrich St. Louis, MO), or maintained in PBS, incubated at 37ºC for 30 minutes. Total RNA was extracted, reverse transcribed, and real-time PCR was carried out using the RT2 Profiler™ human Innate & Adaptive Immune Responses PCR Array containing 84 genes, as recommended by the manufacturer (Qiagen). The Livak method was used to calculate fold change (FC >2 considered significant). The analysis showed that 25 genes out of 84 genes were affected by treatment with eugenol. Among 25 genes, 19 were upregulated, and 2 genes were downregulated. The highest up-regulated and down-regulated genes following exposure to eugenol was IL23A and Interferon Regulatory Factor 7 (IRF7), respectively. The upregulation of the IL-23A gene expression by exogenous eugenol may be important in the production of pro-inflammatory cytokines and warrants further studies to investigate the mechanism involved. Interferon Regulatory Factor 7 is a critical regulator of type I interferon production and plays an important role in innate immune responses. The observed transcriptional expression of IL23A and IRF7 by eugenol provides an insight into immune modulation in cow blood.

Оценка мясной продуктивности бычков голштинской породы в условиях Нижнего Поволжья

Aim. To study the influence of linear affiliation on the meat productivity of Holstein gobies. Materials and Methods. The experimental part of the work was carried out in 2019-2020 on the territory of a dairy unit for milk production, “Donskoe” farm enterprise, LLC, Kalachevsky district, the Volgograd region. For the experiment, 3 groups of 10 heads were formed according to the principle of analogues of purebred Holstein bulls from three lines: Reflection Sovering, Vis Back Ideal, and Montvik Chieftain. During the scientific and economic experiment, the technology of keeping animals used in beef cattle breeding was used. Classical and modern zootechnical methods are used in the process of research. The obtained research materials were processed by the method of variation statistics, taking into account the reliability criterion according to the Student, using the Microsoft Excel software package. Results. Studies have established that in order to achieve the desired parameters of the live weight gain of bull calves, it is necessary to drink them with whole milk. During 2.5 months, the experimental animals consumed 198 liters of whole milk per head. To obtain high average daily gains in fat mass from the 6th day of life, starter feed and finely crushed grain were introduced into the diet of steers. The norms of nutrients were determined depending on the planned growth and live weight. The results of changes in absolute indicators of live weight of calves with age showed that calves belonging to the line of Reflection Sovering 198998, with intensive cultivation, grow much faster than their peers from other lines. The results of the control slaughter show that at the age of 13 months, it is possible to obtain sufficiently full-bodied carcasses from animals. The largest carcass weight was observed in the Reflection Sovering 198998 bull calves (240.5 kg), which is 7.7 and 19.1 kg or 3.2 and 8.2% more than in the peers from other groups. When deboning carcasses, it was found that the bulls of the Reflection Sovering 198998 line also exceeded the pulp mass by 4.0 and 14.9 kg or 2.04 and 7.59% (P ≥ 0.99), and the pulp yield by 0.7 and 0.8%. Thus, it is established that the belonging of bull calves to different lines is related to the change in the composition of the carcass, the ratio between muscle, bone and adipose tissue. Conclusion. Scientific and economic experience shows that high indicators of meat productivity can be obtained from German-bred Holstein steers of various linear varieties, which can serve as a necessary reserve for increasing the level of beef production.