Dairy Donkeys Research Articles

Amiata donkey body conformation, udder characteristics, and their relationship with milk yield and quality

To date, no selective actions have been taken to improve milk traits in dairy donkeys, and the characteristics of the udder are not well defined in relation to the productive characteristics. This study aimed at increasing knowledge on Amiata dairy donkey body conformation, udder traits, and their relationship with milk yield and quality. Morphological, udder, and teat measurements and milk evaluations of 45 pluriparous jennies were carried out. The average wither height of the jennies was 126 cm and the chest girth was 148 cm; a large standard deviation of some body measurements was found. Forty-nine percent of the animals showed a moderately developed udder, while most of the jennies had symmetrical half-udders (96%) and the intermammary cleft was clearly visible in 53% of subjects. Correlation analysis indicated that bigger animals tend to have bigger udders, higher teat diameter, and greater distance between teat tips. A positive correlation between the teat length and the milk fat was found ( p < 0.01), which suggests that jennies with longer teats have a better ability to release milk fat. The results of this paper may be useful to define the characteristics of the milking device and address selective choices of the animals.

Impact of Variability Factors on Hair Cortisol, Blood Count and Milk Production of Donkeys.

The increased interest in donkeys because of their milk has led to changes in their farm management. Little is known about the effect of the farming systems on donkey health and welfare. Measuring hair cortisol concentrations is an emerging method to assess stress in animals. To the best of our knowledge, no cortisol assessment has been done on dairy donkeys; similarly, only a few studies have investigated donkey haematological values. The aim of this study was to evaluate the effects of the lactation phase, parity and season on blood parameters, milk yield and quality and hair cortisol in dairy donkeys. Individual samples of milk, blood and mane hair were taken from twenty jennies at 1, 6 and 10 months after parturition. Higher values of hair cortisol were found in the first sampling, suggesting temporary stress during the peri-parturition. The parity influenced the number of blood cells, which was lower in the pluriparous jennies. The season affected milk quality and mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration. The latters might represent the adaptation to the environmental conditions. This study contributes to a better understanding of the biochemical processes occurring in lactating jennies, and to their physiological and wellbeing status.

Effects of the Physiological Status and Diet on Blood Metabolic Parameters in Amiata Dairy Donkeys.

Simple SummaryThis study investigated the physiological responses of donkeys feeding two different diets during maintenance, pregnancy and lactation. To investigate how the metabolic state influences dairy production, we also assessed the relationships between the blood metabolic profile and milk quality. We found that pregnancy and the first months of lactation led to lower feed intake and increases in blood non-esterified fatty acids (NEFAs), which was linked to the difficulty that the jennies have in meeting the higher energy needs. The intake of the concentrate in the diet was associated with the increase in blood glucose, both during maintenance and lactation. Higher NEFA were related to lower de novo synthesized milk fatty acids, whereas higher plasma B-HBA were related to higher somatic cell count. This paper contributes to the knowledge of physiological responses of jennies during milk production phases and provides information for donkey milk producers.Body weight changes and blood metabolic parameters in jennies feeding two different diets and in three physiological statuses were investigated (maintenance vs. pregnancy; maintenance vs. lactation). The relationships between blood metabolic profile and milk quality were also evaluated. Fourteen jennies were allocated to two groups (1: pregnant/lactating; 2: non-pregnant, non–lactating). Pregnant jennies and maintenance jennies (during the first 10-week measurement period) fed a diet consisted of ad libitum grass hay (diet 1); lactating jennies and maintenance jennies (during the last 10-week measurement period) fed ad libitum grass hay plus 2 kg/head/day of concentrate (diet 2). Blood sampling was performed on the jennies of both groups; individual milk samples were also collected during the first 70 days in milk. Higher blood NEFA (p < 0.05) were found in pregnant compared to maintenance jennies (diet 1) (68 vs. 37 μmol/L). Lactating jennies showed higher (p < 0.01) average blood NEFA (268 vs. 26 μmol/L) and glucose (66 vs. 55 mg/dL) compared to the maintenance (diet 2). Blood glucose was positively correlated to milk fat (p < 0.05), while negative significant correlations between de novo milk fatty acids and NEFAs were observed. Positive correlations between plasma B-HBA and somatic cell count (p < 0.01) were also found.

Cyathostomin fecal egg count and milk quality in dairy donkeys.

This study was performed to assess the possible relationship between gastrointestinal strongylid infections and milk quantity and quality in donkeys used for milk production. Individual fecal and milk samples were collected from 16 multiparous lactating jennies of Amiatina breed at their 6th month of lactation. Statistical analysis was carried out between strongylid eggs per gram of faeces (EPG) and milk parameters. All animals were found to shed cyathostomin eggs, with EPG ranging from 150 to 1900. A higher milk production was observed in animals showing an EPG< 250, but this result was statistically not significant. Conversely, significant higher concentrations of milk urea and pH values, and lower concentrations of milk lactose, were observed in jennies with an EPG >1000. In conclusion, results from this study suggest that cyathostomin EPG values > 1000 may affect milk quality in dairy donkeys. Considering the novelty of these findings, more data needs to be collected to determine EPG thresholds to be used as a good marker for parasite infections influencing milk performance and quality in donkeys.

Nutritional Supplements Containing Cardus mariano, Eucalyptus globulus, Gentiana lutea, Urtica urens, and Mallotus philippinensis Extracts Are Effective in Reducing Egg Shedding in Dairy Jennies (Equus asinus) Naturally Infected by Cyathostomins.

The increasing levels of anthelmintic resistance together with the restrictions in the use of drugs in food producing animals have enforced the search for sustainable alternative approaches for parasite control. The current study aimed to investigate the safety and the efficacy of a commercially available phytotherapic formulation against gastrointestinal strongyles in donkeys. Twenty-two Ragusana jennies (2.6 ± 0.5 years old) were assigned to two equal groups. One group was treated with two doses of a phytotherapic supplement Paraxitebio® containing Cardus mariano, Eucalyptus globulus, Gentiana lutea, Urtica urens, and Mallotus philippinensis, 14 days apart (Group A). One group was used as negative control (Group B). Individual fecal samples were collected at the beginning of the study (T−1), and after 7, 14, and 28 days (T7, T14, T28). Blood samples were collected on T−1 and T28 in order to assess changes in donkeys' hematological profile. After the initial rise in EPG values observed on T7, Group A showed a significant EPG decrease with lower eggs per gram (EPG) count compared to Group B on T28 and an overall fecal egg count reduction of 56.9% on the same time-point. Hematological parameters were within the normal physiological ranges for enrolled donkeys. However, significant differences in the values of RBCs, Hb, MCHC, MCV, WBCs, eosinophils, and basophils were recorded between groups after phytotherapic treatments, with Group A showing a general improvement in the hemogram picture. The phytotherapic supplement used in the current study was helpful in controlling intestinal parasites allowing a significant reduction in the fecal egg count 28 days after treatment. Further studies are needed to better explore the specific mode of action of the plant-derived formulation herein tested as well as to encourage their use as tool for the control of equine strongylosis under multimodal integrated approach in dairy donkey farms.

In Vitro Inhibiting Effects of Three Fungal Species on Eggs of Donkey Gastrointestinal Strongyles.

Recently, donkeys have gained popularity mainly due to the use of donkey milk by the cosmetic industry and for human consumption. Gastrointestinal strongyles (GIS) are considered a potential cause of disease and reduced production in infected donkeys. European laws limit the use of anthelmintic drugs for the control of GIS in dairy donkey farms, thus the need to develop alternative control methods. This study aimed to test the in vitro inhibiting effects of three chitin degrading fungi (Scopulariopsis brevicaulis, Metarhizium anisopliae, and Beauveria bassiana) on the hatch and viability of donkey GIS eggs by using the egg hatch test, and to compare their activity to that of Pochonia chlamydosporia. About 150 eggs were added to 0.5 mL of sterile saline solution containing about 1.4 × 108 spores of each fungal species or with 0.5 mL of sterile saline solution only (untreated controls). After incubation, the percentage of egg hatch reduction was calculated, and data were statistically analyzed. All fungi were able to significantly reduce (p < 0.05) the hatch of GIS eggs compared to the untreated controls. Further studies that aim to investigate the efficiency of these fungi in reducing donkey GIS eggs in contaminated environments are encouraged.

A Review of the Appropriate Nutrition Welfare Criteria of Dairy Donkeys: Nutritional Requirements, Farm Management Requirements and Animal-Based Indicators.

Simple SummaryThe increase in dairy donkey farms in Europe, as a consequence of the increasing scientific interest in donkey milk for paediatric nutrition, has led to the need for a better understanding of the welfare of dairy donkeys. Taking into account the Animal Welfare Indicator’s (AWIN) welfare assessment protocol for donkeys, the aim of this review has been to obtain insight into good feeding welfare principles, in order to identify and discuss the nutritional requirements, the farm management requirements and the animal-based indicators that may be used to achieve an overall assessment of the appropriate nutrition welfare criteria of dairy donkeys.Data are available in the scientific literature concerning the quality and usefulness of donkey milk for human consumption. However, there is a lack of studies related to the understanding of the welfare of dairy donkeys. The only attempt, at a European Union level, to assess the welfare of donkeys is that of the Animal Welfare Indicator’s (AWIN) welfare assessment protocol for donkeys, where the appropriate nutrition welfare criteria have been assessed, but only through the evaluation of the body condition score. However, several other indicators that take into account the importance of good feeding welfare principles should be considered for the correct management of dairy donkeys. Therefore, it is hoped that this review of the available scientific literature will be useful to help establish a set of appropriate welfare requirements and indicators for the management of dairy donkeys. The review is aimed at identifying and discussing other requirements and indicators, such as nutritional requirements, farm management requirements and animal-based indicators, which may be important for the correct assessment of the appropriate nutrition welfare criteria and to establish best practices for the feeding of dairy donkeys.

In Vitro Anthelmintic Activity of Saponins Derived from Medicago spp. Plants against Donkey Gastrointestinal Nematodes.

With the aim to find new effective natural compounds for the control of nematodes, the in vitro anthelminthic properties of purified 1% saponins showing different chemical compositions and derived from Medicago sativa (MS), Medicago arborea (MA), Medicago polymorpha cultivar ‘Santiago’ (MPS), M. polymorpha cultivar ‘Anglona’ (MPA), and 1% prosapogenins from M. sativa (MSp), were evaluated and compared. As a source of nematode eggs, pooled fresh fecal samples taken from dairy donkeys naturally infected by gastrointestinal nematodes were used. From fecal samples, eggs were recovered, suspended in deionized water, and used immediately in the bioassay (egg hatch test). The activity of the tested compounds was compared to positive (0.1% thiabendazole) and negative (deionized water and 1% DMSO) controls. All experiments were repeated in triplicate and the obtained data were statistically analyzed. All the tested plant compounds caused a significant (p < 0.05) inhibition of nematode egg hatching (>80%). Moreover, all saponins and prosapogenins showed in vitro anthelmintic properties statistically comparable to that of the reference drug (p < 0.05), except for MPS extract. Obtained results showed that the different Medicago saponins evaluated in this study possess high anthelmintic properties against gastrointestinal nematodes of dairy donkeys, although to a different extent depending on their composition.

A multi-approach study of the performance of dairy donkey during lactation: preliminary results

Donkey milk is known for some nutritional and nutraceutical peculiarities compared to the milks traditionally used for human nutrition. Moreover although the number of studies on donkey milk production has increased the asinine species still remains little investigated. This is the first study providing a multiple assessment approach in order to extend the knowledge on the production of donkey milk, on the haematological and milk cytological parameters during the whole span of lactation. Furthermore, this study characterised the LYZ and OXT genes. Twenty two individual milk and blood samples from Amiatina donkey were taken at one, six and ten months after parturition. Milk total proteins and caseins and ash were significantly higher at the 1 month of lactation while the urea was lower (1.88% 0.95%, 0.41% and 26.08 mg/mL respectively). Whereas lactose and fat did not significantly changed and showed average values of 6.84 ± 0.145% and 0.67 ± 0.546% respectively. pH and titratable acidity were respectively higher (7.20) and lower (0.10 g/l of lactic acid) at 10th month. The mean values of somatic cell count/mL and of milk macrophages were low with maximum value at tenth month. The haematological parameters were stable during lactation except for mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration and platelet count with maximum at sixth month (respectively 20.22 pg, 36.1 g/dl, 281.16 x 109/l). This study also provides for the first time a characterisation of the LYZ gene in the Amiata donkey and describes a first polymorphism at the promoter level.HighlightsThis study extends the knowledge on the production of donkey milk, on the haematological and milk cytological parameters during the whole span of lactation.The results may have economic, health and social impacts since donkey milk is primarily targeted at sensitive consumers.Lysozyme polymorphisms could have association with anti-bacterial activity in milk and the inflammatory response in the mammary gland.

The development of guidelines to improve dairy donkey management and welfare

ABSTRACTDonkey milk is a valuable product for babies suffering from multiple-allergies and cosmetic production; therefore, new dairy donkey farms are opening around Europe. Little information is available for farmers on sustainable production of donkey milk, including animal welfare, milk production, and processing. Targeted dissemination of information on appropriate animal management would assist dairy donkey farmers in preventing welfare problems. This research project aims to develop guidelines on good practice principles for sustainable donkey milk production. Different steps were followed to develop the guidelines:identification of key issues for dairy donkey welfare, analysing the results of previous project and the available scientific literature;systematic review research to select promising solutions for each issue included in the guidelines;stakeholder consultation, in order to increase scientific soundness and to enhance their acceptability throughout the sector;guidelines drafting and revisions by stakeholders;5. guidelines launch.The guidelines ‘Dairy donkeys: good practice principles for sustainable donkey milk production’ were launched in December 2017. They include suggestions derived from scientific literature and/or reported by internationally recognised experts. The guidelines provide clear and helpful advice on good animal management practices for anyone interested in donkey milk production. They comprise the following chapters: ‘Responsibilities’, ‘Feed and water’, ‘Housing and Management’, ‘Donkey health care’, ‘Humane killing’, ‘Appropriate behaviour’, and ‘Milking procedures’. The guidelines, translated in different languages (Italian, Spanish, Portuguese, French, Greek and Chinese Mandarin) are freely available online.HighlightsThe guidelines “Dairy donkeys: good practice principles for sustainable donkey milk production” are freely available online in six languagesThe guidelines provide clear and helpful advice on good animal management practices for anyone interested in donkey milk productionThe guidelines include suggestions derived from scientific literature and/or reported by internationally recognised experts

Survey of biochemical and oxidative profile in donkey foals suckled with one natural and one semi-artificial technique.

Dairy donkey milking procedures require separating foals from their dams for a few hours a day. Artificial suckling in this species is a good technique for improving milk production and foal welfare. The aim of the work is to compare the effect of two different diets on donkey foals when separated from jennies for milking procedures with and without a milk replacer. Forty newborn Martina Franca donkey foals were subdivided into two experimental groups. Both groups were separated from their respective dams from 8.00to 20.00to allow the jennies to be milked. During the separation, all the foals had access ad libitum to water, hay and feed. During the separation period, one group had the availability of a mechanical milk replacer dispenser, so foals were partially artificially suckled (AS), while the other group had no milk replacer available, and so were totally naturally suckled (NS). The AS group had milk replacer availability until 120±7d of life. Both groups were naturally weaned at 168±7d. Blood samples were collected weekly starting from birth until two wks after weaning (i.e. at 182d), from all the foals included in the trial. Almost all the analytes were influenced by suckling technique and age of foals. Alanine-aminotransferase, aspartate-aminotransferase, alkaline phosphatase, NEFA, lipid hydroperoxides, serum proteins showed the greatest differences between the two experimental groups. Separating foals from their dams for 12hdaily for 24 weeks does not lead to pathological subclinical and metabolic conditions, thus confirming the high rusticity and resistance of the donkey.

Evaluation of different habituation protocols for training dairy jennies to the milking parlor: Effect on milk yield, behavior, heart rate and salivary cortisol

The aim of the trial was to apply different habituation protocols for machine milking to never machine-milked donkeys observing the effect on behavior, heart rate, salivary cortisol concentration and milk yield. Sixty lactating dairy donkeys were studied; the animals were divided into three groups of 20 each to evaluate whether a habituation protocol for dairy donkeys could help ensure good animal welfare and production. None of the animals had been subjected to mechanical milking procedures and had never entered the milking parlor. The trial lasted 15 days (two sessions per day), for a total of 30 experimental sessions: 9 days of training sessions for Groups A and B, and 6 days of milking sessions for all three Groups. Behavioral patterns, heart rate (HR) and salivary cortisol were measured during each experimental session. Two groups (i.e. A and B) received two different pre-milking habituation handling protocols; as the control group, Group C received no habituation and was directly milked in the milking parlor. For the first 3 days (phase 1; sessions 1–6), Groups A and B both passed through the milking parlor while the milking machinery was switched off. In the next 6 days (from Days 4–9, sessions 7–18), Group A donkeys received a “less intense” treatment, involving 2 days passing through the milking parlor where the machinery was switched on during phase 2 (sessions 7–10) and, for the next 4 days being stopped in the milking stall (phase 3; sessions 11–18). Meanwhile, the donkeys in Group B, on all 6 days, were confined in the milking stall, and their udders were brought into contact with the milking cluster, which was turned on at the time. Subsequently, all groups involved in the trial were machine-milked in the milking parlor for 6 days (phase 4; sessions 19–30). Donkeys that received the pre-milking habituation handling (Groups A and B) showed less reactive behaviors (kicks and steps) and incoming stops when milking started compared to the control group (P < 0.01). Differences in HR were observed particularly between the control and the other groups (P < 0.05). Results suggest that a pre-milking habituation protocol providing short contact with the milking cluster (as in Group B) can result in better response from the animals with less reactive behavior, lower heart rate increase and higher milk production.

Evaluation of levels of Essential Elements and Heavy Metals in Milks of Dairy Donkeys, Goats and Sheep in Turkey

Evaluation of levels of Essential Elements and Heavy Metals in Milks of Dairy Donkeys, Goats and Sheep in Turkey

Microbiological safety and quality of Italian donkey milk

AbstractConsidering that few data are currently available about donkey milk microbiological quality and given the spread of food‐borne outbreaks strongly linked with the consumption of raw milk, the aim of the present study was to investigate the microbiological quality and the food safety of Italian donkey milk. Total viable count (TVC) and coagulase‐positive Staphylococcus were evaluated to verify the process hygiene criteria for donkey's milk. The presence of Salmonella spp., Listeria monocytogenes, Cronobacter sakazakii, E. coli O157, and Campylobacter spp. were evaluated to estimate its food safety criteria. The study revealed that the TVC values were compliant with European legislation. Salmonella spp., Listeria monocytogenes, Coagulase‐positive Staphylococcus and Cronobacter sakazakii were not detected. However, the presence of E. coli O157 and Campylobacter coli was observed, highlighting an important health‐related issue, underlining the need to implement an adequate control plan by performing intensive and continuous monitoring in order to guarantee human health.Practical applicationsDonkey's milk is considered a “pharmafood” due to its nutritional, nutraceutical, and functional properties. Indeed, it has been configured as a valid alternative foodstuff for children affected by cow's milk protein allergy, atopic, and inflammatory diseases due to its distinct chemical composition and consequently particular nutritional properties. However, undesirable pathogens responsible for food‐borne diseases harmful to humans have been detected. High hygiene standards should be followed along raw milk chain to avoid potential hazardous pathogenic bacteria spreading to consumers and microbiological parameters and criteria need to be established in raw donkey milk for human consumption. Specific sanitary control plans are required for authorized dairy donkey farms.

Effect of farming system on donkey milk composition.

Donkey milk is considered as a functional food for sensitive consumers, such as children who are allergic to cow milk. No information is available regarding the effect of farming systems on the quality of donkey milk. The present study aimed to evaluate the effect of the farming system and lactation stage on donkey milk with respect to gross composition, as well as fat-soluble vitamins and fatty acids (FA). Individual milk samples were collected from lactating jennies (n = 53) on the six of the largest farms located in North West Italy. The performance of lactating jennies, herd characteristics and feeding strategies were recorded at each milk sampling. The gross composition of the milk, along with the fat-soluble vitamin content, differed in accordance with the farming system. The lactation stage had limited effects on milk quality. A higher milk fat content corresponded to a higher amount of fresh herbage proportion in the diet, with the highest polyunsaturated fatty acid (PUFA), C18:1c9, C18:3n-3, n-3 FA, retinol and α-tocopherol content and the lowest concentrations of the FA that are less favorable for human health in the milk of animals fed on only forage diets. Extensive farming of dairy donkeys increased the fat content and fat-soluble vitamin concentration of milk and also altered the FA composition to a more favorable profile for human nutrition. © 2017 Society of Chemical Industry.

Effects of management practices on the welfare of dairy donkeys and risk factors associated with signs of hoof neglect.

This Research Paper aimed to investigate donkey welfare in dairy husbandry systems and to identify the potential factors affecting it at animal level. In 2015, twelve dairy donkey farms (19–170 donkeys per farm, mean = 55 ± 48), distributed throughout Italy, were visited. On each farm, the Animal Welfare Indicators (AWIN) welfare assessment protocol for donkeys was used by two trained assessors to evaluate the welfare of animals for a total of 257 donkeys assessed. The protocol includes animal-based indicators that were entered in a digitalised system. Prevalence of different scores at individual, farm and category level were calculated. Farmers were asked to fill out a questionnaire including information regarding the management of donkeys and their final destination. Answers to the questionnaire were then considered as effects in the risk factor analysis whereas the scores of the animal-based indicators were considered as response variables. Most of the donkeys (80·2%) enjoyed a good nutritional status (BCS = 3). 18·7% of donkeys showed signs of hoof neglect such as overgrowth and/or incorrect trimming (Min = 0% Max = 54·5%). Belonging to a given farm or production group influenced many of the welfare indicators. The absence of pasture affected the likelihood of having skin lesions, alopecia, low BCS scores and a less positive emotional state. Lack of routine veterinary visits (P&lt; 0·001) and having neglected hooves (P&lt; 0·001) affected the likelihood of being thin (BCS &lt; 3). Belonging to specific production groups, lack of access to pasture and showing an avoidance reaction to an approaching human (AD) resulted in risk factors associated with a higher prevalence of signs of hoof neglect. Our results support the idea that lack of knowledge of proper donkey care among owners was behind many welfare issues found.

A Preliminary Assessment of HTST Processing on Donkey Milk.

Due to increasing attention from consumers on non-bovine milk types, and to the increase in the number of small dairy donkey farms in Italy, farmers require more advanced and reliable processing devices, in order to guarantee a safe product of high quality. To this aim, a new small-scale High-Temperature Short-Time (HTST) pasteurizer (72 °C for 15 s), prototyped by the authors, was tested on donkey milk. The efficacy of the HTST device was tested on raw donkey milk microflora by enumeration of total aerobic bacteria, Enterobacteriaceae and Bacillus cereus. The biochemical quality was assessed by determining the protein profile by monodimensional electrophoresis and by measuring lysozyme activity. The HTST apparatus was able to reduce the total bacteria count, and to completely eradicate Enterobacteriaceae. Bacillus cereus, when present, was decreased with low efficiency. Changes in the protein profile were observed in milk pasteurized in accordance with both processes, although HTST seemed to limit casein degradation. Lysozyme activity was not substantially affected in comparison to raw donkey milk. In conclusion, a tailored small-volume HTST device could be safely applied to pasteurize donkey milk in on-farm pasteurization processes on small dairy donkey farms.

Management Practices and Milk Production in Dairy Donkey Farms Distributed Over the Italian Territory

AbstractLimited information is available about the actual management characteristics of dairy donkeys in Southern Europe. The aim of the present study is to describe animal management of dairy donkey farms in Italy. Twelve farmers were asked to answer a questionnaire on the management of their animals and their farms distributed over the Italian territory. Six farms grouped their animals in paddocks according to the production characteristics (e.g. lactating, dry, stallions); three farms housed the stallions in single boxes. Most of the visited farms were family run and the number of animals cared for by a single person varied from five to 103 animals. All the farms but one performed mechanical milking with a modified goat milkmaid. Vaccinations were regularly performed only on two farms. All the foals received colostrum and suckled from their own mothers. Foals were nursed by their mother until 6-12 months old. During the separation period before milking, foals were usually (83%) housed in paddocks near their mothers with the possibility of visual and/or tactile contact, however such separations could be for up to 12 hours (17%). Even though the assessed sample was small, considerable differences were seen between farms, likely due to lack of uniform information available for the farmers. The adoption of scientific based procedures is suggested in order to improve both animal welfare and milk quality.

Dairy donkey: an alternative building layout

In Italy, donkey dairies are growing in popularity to produce an alternative milk source for human infants. The use of donkey milk is not limited to the paediatric field, being used in geriatric nutrition and in the cosmetic industry. In 2010, the Department of Veterinary Science, University of Pisa, was involved in a project supported by the Tuscany Region Rural Development Fund 2007- 2013 dealing with the creation of a donkey milk productive chain. The project has as its main partner the Bandite di Scarlino, an agro-forestry complex of considerable size. It covers approximately 9000 hectares, which fall in Scarlino, Castiglione della Pescaia, Follonica and Gavorrano municipalities, under Tuscany regional control. The so-called FILAMI project (Filiera Latte Asino Amiatino, acronym of the goal of the project in Italian) had among its goals helping preserve an indigenous breed of donkeys called Amiatina. Salimei (2011) assessed that the presence of the foal during milking does not affect milk ejection in jennies (the female donkey) adapted to the milking procedure, but when foals are not present the milking routine is more manageable for the animals and for optimal milk harvest; in accordance, in the present farming system the jenny has been kept separately from the foal during the milking session. To this purpose, the stable, the milking parlour and the paddocks must be well organised with a proper milking routine. Milking facilities must be designed for the milk treatment and storage at low temperature or for freezing. Currently, the milk undergoes a pasteurisation cycle for human consumption, or directed to the cosmetic industry. Moreover, the milking facilities should fulfil the creation of a milk collection centre in order to play an important role between the dairy donkey farms and the dairy industry. In this paper, the technical and technological aspects of the donkey stable, paddocks, milking parlour, mobile milking unit, and processing plants have been described and the main issues addressed.

Amiata Donkey Milk Chain: Animal Health Evaluation and Milk Quality.

This study presents an investigation of Amiata donkey health and quality of milk for human consumption. Thirty-one lactating dairy jennies were examined. The following samples were collected: faecal samples from the rectum of animals for parasitological examination; cervical swabs for the detection of bacteria causing reproductive disorders; and blood samples for serological diagnosis of main zoonotic (Brucella spp., Leptospira spp.) and donkey abortion agents (Brucella spp., Leptospira spp., Salmonella abortus equi, Equine viral arterithis virus, Equine herpesvirus type 1). In addition, individual milk samples were collected and analysed for mastitis-causing pathogens and milk quality. Regarding animal health, we detected a high prevalence of strongyle parasites in donkeys. It is very important to tackle parasitic diseases correctly. Selective control programmes are preferable in order to reduce anthelmintic drug use. For dairy donkeys, withdrawal periods from anthelmintic drugs need to be carefully managed, in accordance with EU and national regulations. The isolation of Staphylococcus aureus in milk highlights the importance of preventing contamination during milking, by adopting appropriate hygiene and safety practices at a farm level. Lysozyme activity was high compared to cow’s milk, contributing to the inhibitory activity against certain bacteria. Donkey milk was characterised by high lactose content, low caseins, low fat, higher levels of unsaturated fatty acids compared to ruminant milks. Unsaturated fatty acids and omega 3 fatty acids in particular have become known for their beneficial health effect, which is favourable for human diet. These characteristics make it suitable for infants and children affected by food intolerance/allergies to bovine milk proteins and multiple food allergies as well as for adults with dyslipidemias. It is also recommended to prevent cardiovascular diseases.