Milk Replacer Research Articles

Effect of pre-farrowing hygiene routine (sub-standard vs. optimal) and creep feeding regime (dry pelleted starter diet vs. liquid mixture of milk replacer and starter diet) on post-weaning intestinal parameters and growth to slaughter in pigs.

The objective was to evaluate the effect of providing dry pelleted starter diet (DPS) or a liquid mixture of milk replacer and starter diet (LMR+S) to suckling pigs housed in farrowing pens of sub-standard or optimal hygiene conditions on pig growth to slaughter, and post-weaning (PW) intestinal parameters. On day (d) 107 of gestation, 87 sows were randomly allocated to one of four treatments in a 2×2 factorial arrangement. The factors were creep feeding (DPS or LMR+S) and pre-farrowing hygiene routine (SUB-STANDARD or OPTIMAL). Pigs were provided with DPS (manually) from d11 to weaning (at d28±1.2 of age) or LMR+S using an automatic liquid feeding system from d4 to weaning. The SUB-STANDARD hygiene routine (pens washed and dried for ~18h, sows not washed or disinfected) and the OPTIMAL hygiene routine (pens pre-soaked, detergent applied, washed, dried for 3 days, chlorocresol-based disinfectant applied, dried for 3 more days and sows washed and disinfected with Virkon) were used to obtain SUB-STANDARD or OPTIMAL hygiene conditions, respectively in farrowing rooms prior to entry of sows. Microbiome analysis was performed on fecal samples from 8 focal pigs/treatment, before weaning and at d21 and d114 PW. On d4 PW, 10 pigs/treatment were euthanized to collect intestinal tissue and digesta samples for histological, enzyme activity and microbiome analysis. Feeding LMR+S to pigs born into the OPTIMAL hygiene increased total dry matter intake compared to all of the other groups (P ≤ 0.05) and increased weaning weight compared to DPS feeding under both OPTIMAL and SUB-STANDARD hygiene conditions (P ≤ 0.05). Pigs from OPTIMAL farrowing pens had lower clinical cases of disease, diarrhoea prevalence and were slaughtered 3.8 days earlier than those from SUB-STANDARD farrowing pens (P ≤ 0.05). Suckling piglet mortality was reduced with LMR+S (P ≤ 0.05). On d4 PW, jejunal and ileal villus height were increased by OPTIMAL hygiene and ileal sucrase activity was increased by LMR+S (P ≤ 0.05). On d4 PW, LMR+S-fed pigs from OPTIMAL farrowing pens had lower relative abundance of Clostridium_P in the jejunum. In conclusion, the OPTIMAL hygiene routine increased pre-weaning LMR+S feed intake, reduced clinical cases of disease, improved intestinal structure and reduced the weaning to slaughter duration, while LMR+S feeding increased weaning weight, intestinal maturity and reduced pre-weaning mortality.

COMPARISON OF HAND-REARED PRONGHORN (ANTILOCAPRA AMERICANA) MILK REPLACER COMPOSITIONS AND GROWTH RATES.

Hand-rearing protocols for nondomestic ungulates used for research, zoological parks, and reintroduction to the wild are evaluated on the basis of implementation practicality, gastrointestinal disturbances, survival to weaning, and growth rates compared with those of dam-raised individuals. Although species-specific protocols utilizing milk replacers formulated for nondomestic ungulates have been reported, no such protocols have been published for pronghorn (Antilocapra americana). Five pronghorn fawns were fed Day One 30/40 Black Tail Deer plus Lactobacillus acidophilus probiotics four times per day with Day One Ultra Boost (all products from Fox Valley Animal Nutrition, Inc, Huntley, IL 60142, USA) added at one feeding per day and weighed twice per week for up to the first 16 wk of life. All fawns survived beyond weaning and four out of five fawns experienced a combined total of seven bouts of diarrhea. Based on a linear mixed-effects model, the growth rate of pronghorn fawns was 178 g/d (SE, 14.8; 95% CI, 149-207). The observed growth rate was greater than or similar to that of individuals fed evaporated milk five times per day, a dam-raised singlet, and a singular set of dam-raised twins. Despite small sample sizes, this protocol shows promise as a method for hand-rearing pronghorn at growth rates comparable to those of dam-raised fawns.

Assessing Compliance: Violations of WHO Code in Breast Milk Substitute Marketing, Ecuador.

Breast milk substitute (BMS) marketing significantly influences global infant feeding practices. Ecuador, like many countries, seeks to regulate these promotions under the WHO's International Code of Marketing of Breast-Milk Substitutes. This cross-sectional analysis assessed BMS marketing compliance with WHO's Code in Ecuador. Surveys were conducted with mothers (n = 330) and healthcare professionals (n = 66), complemented by observations at health facilities (n = 33) and retail outlets (n = 44). Media monitoring and product labelling evaluations were also conducted. The study revealed widespread exposure to BMS marketing outside health facilities (91.21% of mothers). Promotional activities targeted healthcare professionals, with significant interactions involving free supplies (26.09%) and gifts (21.74%). Retail outlets prominently displayed BMS promotions, often featuring discounts (95%). Compliance with labelling criteria was notably low, particularly concerning nutrition and health claims (39%). TV emerged as the dominant platform for BMS advertising, with 2884 ads aired over 16 h and 24 min, totalling $1,876,915.50 in expenditures. Digital platforms also featured BMS ads, with significant engagement on social media (533,845 interactions). This study reveals widespread violations of the WHO Code in Ecuador, emphasizing the need for stronger regulations and targeted education for healthcare professionals and the public to protect infant health and promote breastfeeding. Despite existing regulations, the pervasive advertising and substantial investment in BMS marketing across various media underscore significant enforcement gaps. To effectively safeguard maternal and child health, Ecuador must fully incorporate and rigorously enforce all Code recommendations within its national legislation.

Enhancing the Efficiency and Sustainability of Producing Curcumin‐Infused Plant‐Based Milk Alternatives With a Two‐in‐One Post pH‐Driven Processing Strategy

ABSTRACTIncreasing food sustainability and health benefits is essential to meet the demands of a growing global population while minimizing environmental impact. This study used a green two‐in‐one post pH‐driven processing strategy to develop a sustainable and healthy plant‐based milk alternative. It achieved both extraction and encapsulation in one step by directly incorporating the health‐promoting curcumin from turmeric into soymilk. A high processing efficiency was observed, 94.2% ± 1.6%, with a high extraction efficiency of 96.4% ± 0.5%. Using raw turmeric instead of a purified curcumin significantly enhanced the sustainability in the use of raw materials, for example, reducing the CO2‐eq emissions by 22 times and energy use by 10 times, even with a very small percentage of curcumin (∼0.03 wt%) in the formulation. This strategy underscores the importance of using raw materials and minimizing processing steps to develop more sustainable foods. Additionally, the incorporation of curcumin was found to impart a yellow color to soymilk. No significant changes were observed in other physicochemical properties like particle size, zeta potential, and melting behavior, as most curcumin molecules were encapsulated within the lipid phase of soymilk. The curcumin‐infused soymilk powders also maintained excellent storage stability for 1 month under freezing temperature.

Modulation of the Plasma Lipidomic Profile in Piglets Fed Polar Lipid-Rich Diets

Background: Polar lipids from dairy are novel sources of energy that may replace other dietary lipids and impact plasma lipidomic profiles in piglets. This study evaluated the impact of feeding diets rich in polar lipids on the plasma lipidome of piglets during the weaning period. Material and Methods: Weaned male piglets (n = 240; 21 days of age; 6.3 ± 0.5 kg of BW) were blocked by initial weight and distributed into 48 pens of five animals each in a complete randomized block design with a 2 × 3 factorial arrangement of treatments as follows: a plant-based diet rich in neutral lipids from soybeans (24 pens; SD) or a polar lipid-rich diet by-product of cheese making (24 pens; PD) from weaning until the 21st day of the nursery phase. Within each diet group, animals received one of three milk replacers (MR; 0.5 L/d/animal) for the first 7 days after weaning: (1) commercial MR containing animal and coconut lipids (CO); (2) polar lipid-based MR (PO); or (3) soybean lipids-based MR (SO). Results: The PD diet group increased the plasma concentrations of sphingolipids, phospholipids, and cholesterol esters, but did not impact the concentrations of glycerolipids (GLs). Both the PO and CO milk replacers increased the plasma concentrations of ceramide, acyl-chain phosphatidyl choline, and cholesterol esters. The plasma concentrations of GLs containing 18-carbon fatty acids such as 18:0, 18:1, 18:2, and 18:3, were higher in SD, whereas GLs containing 16:0 and 20:3 were higher in PD. Conclusions: In summary, the diet lipid type significantly modulated the plasma lipid composition in piglets 7 days after weaning. The dietary inclusion of polar lipids in diets for growing pigs can modulate the plasma lipidomic profile, relative to plant-based diets rich in soybean lipids. Cost may be a major consideration when using these lipids in pig diets. Their health benefits need to be further characterized in other models of stress and inflammation.

A multicriteria analysis of meat and milk alternatives from nutritional, health, environmental, and cost perspectives

Reducing meat and dairy intake has been identified as a necessary strategy for mitigating the high environmental impacts food systems are currently having on climate change, biodiversity loss associated with land-use changes, and freshwater use. Having a choice of dedicated meat and milk replacements available to consumers can help in the transition toward more plant-based diets, but concerns about nutritional and health impacts and high costs can impede uptake. Here, we conduct a multicriteria assessment of 24 meat and milk alternatives that integrates nutritional, health, environmental, and cost analyses with a focus on high-income countries. Unprocessed plant-based foods such as peas, soybeans, and beans performed best in our assessment across all domains. In comparison, processed plant-based products such as veggie burgers, traditional meat replacements such as tempeh, and plant milks were associated with less climate benefits and greater costs than unprocessed foods but still offered substantial environmental, health, and nutritional benefits compared to animal products. Our findings suggest that a range of food products exist that when replacing meat and dairy in current diets would have multiple benefits, including reductions in nutritional imbalances, dietary risks and mortality, environmental resource use and pollution, and when choosing unprocessed foods over processed ones also diet costs. The findings provide support for public policies and business initiatives aimed at increasing their uptake.

Opportunities and challenges of plant proteins as functional ingredients for food production

Consumer interest in meat and dairy alternatives drives demand for plant-based protein ingredients. While soy and gluten dominate the market, there is a trend to explore alternative crops for functional ingredient production. The multitude of ingredients poses challenges for food manufacturers in selecting the right protein. We investigated 61 commercially available protein ingredients from various sources, categorizing them based on their protein content into protein-rich flours (protein content less than 50%), protein concentrates (protein content between 50% and 80%), and protein isolates (protein content higher than 80%). Methionine, cysteine, and lysine were decisive for the amino acid score, which even varied between ingredients produced from the same raw material. Such differences were also observed in the protein solubility profiles, characterized by their raw material-specific protein pattern. By focusing on soy, pea, and fava bean ingredients, the broad spectra of emulsifying and foaming properties were illustrated. These ranged from non-emulsifying and non-foaming to high emulsifying capacities of 737 mL/g ingredient and foaming activities of 2,278%, accompanied by a foam stability of 100%. Additionally, we demonstrated that the functionality of ingredients obtained from different batches could vary by up to 24% relative SD. Protein solubility, powder wettability, color, and particle size were determined as key properties for the differentiation of soy, pea, and fava bean protein ingredients by principal component analysis. In our study conclusion, we propose essential measures for overcoming challenges in protein ingredient production and utilization to realize their full potential in fostering sustainable and innovative plant-based food production.

Performance, nutrient digestibilities, and metabolic profiles of Holstein calves fed whole milk or milk replacer at two feeding levels.

The macronutrient composition of common milk replacers (MR) largely differs from bovine whole milk (WM). These differences are more nutritionally relevant when calves receive higher milk allowances. Therefore, the present study compared fresh WM with a conventional MR fed at 2 level of feeding allowances evaluating growth performance, apparent total-tract nutrient digestibilities, nitrogen (N) balance, and serum metabolite profiles in calves. Forty-eight newborn Holstein male calves (1.96 ± 0.99 d of age; 45.0 ± 4.37 kg BW) were blocked by arrival date and age. Calves in each block were then randomly assigned to the 4 treatments (n = 12/treatment): MR or WM at a high (9.0 L/d) or low feeding allowance (4.5 L/d) fed 3 times daily. Calves were weaned between wk 6 and 10 following the introduction of the starter feed in wk 6. The study ended 13 wk after the arrival at the facility. Blood and body weights were collected on arrival, and weekly thereafter. Total 24h-collection periods of feces and urine was performed at wk 2, 4, 5, 7, 9, 11, and 13. Remarkably, no interactions were observed between liquid feed and level of supply. Calves fed MR had higher ADG during the pre-weaning phase than to calves fed WM. However, calves fed WM had an increased growth rate post-weaning, resulting in no difference in BW at the end of the study. Calves fed WM had a higher apparent crude fat digestibility during the weaning period. Metabolic N efficiency, expressed as N balance relative to digestible N supply, was higher during the pre-weaning phase, but tended to be lower during the post-weaning phase for calves fed MR as compared with calves fed WM. In the pre-weaning period, serum glucose, BHB, nonesterified fatty acids (NEFA), total protein (TP), urea, albumin, globulin, triglyceride (TG), and cholesterol were influenced by liquid feed source. Carry-over effects of milk source on TG and TP were observed during the post-weaning period. As expected, calves fed a high-milk allowance showed better ADG at pre-weaning and were consistently heavier throughout the study than calves fed a low-milk allowance. Feeding a high-milk allowance did not negatively affect nutrient digestibility during the post-weaning phase. In the pre-weaning phase, milk allowance affected glucose and cholesterol metabolism, whereas TG, TP, albumin, and NEFA concentrations were affected during the post-weaning period. From a single oral dose of indigestible markers (Cr-EDTA, lactulose, and D-mannitol), calves fed a high-milk allowance showed greater urinary recovery of all markers after 6h of collection, but not after 24h. Calves fed a high-milk allowance had a higher proportion of days with lower fecal scores during the pre-weaning phase. In conclusion, the serum metabolite profiles of calves throughout the study were highly influenced by milk source. Higher milk allowance increased growth performance and had no adverse effects on starter feed intake or nutrient digestibility in the post-weaning phase. The multiple effects described for milk source and level of supply were overall independent, presenting no relevant interactions.

Exploring baseline behaviour in group-housed, pre-weaned dairy calves

With increasing public concern for farm animal welfare, understanding their current welfare status is paramount. Animal welfare can be inferred from their behaviour, as behaviour represents the combination of internal and external cues. The aims of this study were to quantify a behavioural baseline for group-housed, pre-weaned dairy calves that were reared under conventional management conditions, and to determine how different internal (i.e. age) and external (i.e. temperature) factors affected this behaviour. Female dairy calves (n=47) were allocated to 1 of 3 pens based on birth date and reared under conventional Irish management conditions; after 3-4 d in individual pens, calves were moved into group pens where they had ad libitum access to water, concentrates, and forage (first barley straw, then hay). Milk replacer (6L/d) was fed through an automatic milk feeder; calves were gradually weaned from day 42 to 84. A 24h period/week of video recording was used for behaviour scoring for 8 consecutive weeks (scan sampling at 10-min intervals). Behaviours included posture (lying or standing) and activity (17 behaviours). Calves were scored for clinical health twice weekly and only healthy calves were used in the analysis (n=39). Behaviour proportions were analysed using generalised linear mixed models. Proportion of time spent lying decreased as calves aged (week 1 vs. 9; percentage mean ± standard deviation; 79.8 ± 4.04 vs. 72.1 ± 6.52%; P=0.004), while time spent ruminating (2.0 ± 2.51 vs. 14.1 ± 8.72%; P<0.001), eating bedding (0.8 ± 1.16 vs. 6.1 ± 4.66%; P<0.001), eating forage (0.9 ± 1.20 vs. 1.8 ± 1.81%; P=0.007), and eating concentrates (0.5 ± 1.15 vs. 2.2 ± 1.72%; P=0.018) increased with age. On days when the minimum shed temperature was <4°C compared to >6°C, calves spent more time lying (75.9 ± 5.27 vs. 72.3 ± 5.78%; P<0.001) and less time eating concentrates (0.8 ± 1.11 vs. 1.4 ± 1.49%; P=0.035), eating forage (0.8 ± 0.91 vs. 1.5 ± 1.57%; P=0.005), eating bedding (2.7 ± 2.87 vs. 4.0 ± 4.78%; P=0.003), and walking (1.5 ± 1.20 vs. 2.0 ± 1.40%; P=0.017), independent of age. These findings provide a normal behaviour baseline for future calf behaviour studies and highlight potential areas of improvement in current, conventional calf rearing practices.

Early-life Clostridium butyricum supplementation improved rumen development and immune by promoting the maturation of intestinal microbiota

This study evaluated the effects of Clostridium butyricum supplementation on rumen fermentation, serum immunoglobulins, and fecal microbiota in dairy calves. Seventy-five 3-days-age calves were divided into five groups (n = 15 per group) receiving 0, 0.2, 0.4, 0.8, and 1.6 g/day of C. butyricum freeze-dried powder (10ˆ10 CFU/g) in milk replacer. Rumen fluid and blood samples were collected at various intervals, and fecal samples from eight calves per group were collected at 3, 28, and 42 days. C. butyricum supplementation showed a negative correlation with rumen pH (P = 0.032) and positive correlations with acetate/propionate (P = 0.007) and acetate + butyrate/propionate (P = 0.022). It had quadratic effects on rumen acetate (P = 0.029) and isobutyrate (P = 0.021). IgA concentration increased with dose (P = 0.002), and dose-time interactions affected serum IgM (P = 0.013) and IgG (P < 0.001). C. butyricum did not influence fecal microbiota diversity but increased the abundance of Helicobacter japonicus, Weissella viridescens, Leuconostoc mesenteroides, Leuconostoc citreum, while negatively correlating with Streptococcus hyointestinalis (P < 0.05). Microbiota functions related to galactose metabolism, and fatty acid degradation decreased with age (P < 0.05), while fatty acid biosynthesis and glycolysis/gluconeogenesis increased (P < 0.05). L. mesenteroides, and L. citreum, and negatively correlated with galactose metabolism and fatty acid degradation but positively with fatty acid biosynthesis (P < 0.01). C. butyricum supplementation variably impacted rumen pH, acetate/propionate, and acetate + butyrate/propionate, particularly on day 42 versus day 60, affecting acetate, butyrate, and isovalerate differently over time. Our findings suggest that early-life C. butyricum supplementation may promote the maturation of immunity and intestinal microbiota of pre-weaning dairy calves, the optimal dose was 0.8 to 1.6 x 10ˆ10 CFU/d.

Administration of a diabetes-specific formula can improve postprandial glycemic control and delay insulin use in gestational diabetes mellitus: a randomized controlled trial from two centers

Background and AimsThere have been limited studies on the application of a diabetes-specific formula in gestational diabetes mellitus (GDM), the role of which has not been well studied. We explored the effect of a diabetes-specific formula on blood glucose levels, insulin use and pregnancy outcomes in GDM patients. MethodsIn this randomized controlled study, 112 GDM patients were randomly assigned to the intervention group (56) and the control group (56). Both groups received individualized dietary counseling. The intervention group consumed a soy-protein-based, high-monounsaturated-fatty-acid, and multi-fiber diabetes-specific formula as milk replacement for breakfast and an extra meal after dinner. All participants were followed up every two weeks until delivery. The blood glucose levels, insulin use and pregnancy outcomes between the groups were compared. ResultsCompared to the control group, the intervention group had significantly lower 2h postprandial blood glucose levels after breakfast (5.84±0.56 vs. 6.15±0.44mmol/L, p=0.008), and exhibited higher postprandial time in range values (83.80% vs. 78.95%, p=0.045). The intervention group used insulin later (33 vs. 28 weeks, p=0.015) and for fewer days (36 vs 78 days, p=0.024), but no differences in the proportion, dose or frequency of insulin use between the groups. There were no significant differences in pregnancy outcomes between the groups. ConclusionsThe diabetes-specific formula significantly decreased postprandial blood glucose levels and improved postprandial glycemic control in GDM patients. Moreover, it delayed the initiation of insulin use and reduced the duration of insulin therapy. Our findings may offer a potential new approach for achieving better personalized blood glucose control in GDM patients. Registration number of Clinical TrialNCT03957603 (registered at ClinicalTrials.gov).

Effect of nonsteroidal anti-inflammatory drugs on neonatal calf diarrhea when administered at a disease alert generated by automated milk feeders.

The objective of this randomized clinical trial was to assess whether early intervention with a nonsteroidal anti-inflammatory drug (NSAID) following a disease alert generated by automated milk feeders could reduce diarrhea severity and improve performance in dairy calves. Seventy-one Holstein calves were enrolled on an automated milk feeder (recorded milk intake and drinking speed) at 3 d of age and received up to 15 L/d (150 g/L) of milk replacer until 35 d of age. An alert that was previously validated as diagnostically accurate to identify calves at risk for diarrhea was used using automated milk feeder data (≤60% rolling dividends in milk intake and/or drinking speed over 2 d). At their first alert, calves were randomly allocated to receive a single subcutaneous injection of meloxicam (Metacam, Boehringer Ingelheim) at a rate of 0.5 mg/kg of body weight (NSAID) or an equal volume of a placebo, saline (CON). Fecal consistency was scored daily, and calves were diagnosed with diarrhea when they had loose feces for ≥2 d or watery feces for ≥1 d. Body weight was recorded at birth and weekly thereafter. A subset of calves (n = 32) were fitted with IceQube pedometers at 3 d of age to measure activity behaviors (lying time and step count). Mixed linear regression models were used to assess the association of study treatment with the duration of diarrhea after the alert; and to evaluate the association of study treatment with milk intake, drinking speed, lying time, overall activity for 5 d following the alert, and ADG for 3 wk following the alert. On average calves triggered an alert at (mean ± SD) 9.3 ± 2.3 d of age and were diagnosed with diarrhea at 9.6 ± 2.1 d of age. Diarrhea duration was similar between treatments (NSAID: 2.85 vs. CON: 2.94 ± 0.37 d) as were feeding behaviors [Milk intake (NSAID: 8.2 vs. CON: 8.1 ± 0.4 L/d) and drinking speed (NSAID: 0.38 vs. CON: 0.37 ± 0.02 min/L)]. Treatment was also not associated with ADG for the 3 wk after the alert (NSAID: 0.97 vs. CON: 0.97 ± 0.06 kg/d). However, calves provided an NSAID had reduced odds of being treated with electrolytes (OR = 0.32, 95% CI: 0.10 - 0.98). In addition, calves provided an NSAID spent less time lying (NSAID: 17.64 vs. CON: 18.17 ± 0.19 h/day) and performed more steps over the 5-d following the alert (NSAID: 789.1 vs. CON: 628.0 steps/d), suggesting that CON calves may have been more lethargic. Overall, providing an NSAID at the time of a diarrhea alert did not affect diarrhea duration, feed intake, or growth. However, providing an NSAID increased activity in the 5 d following the alert, which may have reduced pain and symptoms of lethargy, indicating a milder response to the disease. We suggest that providing an NSAID at the time of diarrhea alert had little benefit on the calf; however, further work is needed to understand behaviors associated with malaise and pain in calves with diarrhea, and the efficacy of NSAID under different management conditions.

Bile salt inclusion rates in milk replacer fed to neonatal calves

Bile salt inclusion rates in milk replacer fed to neonatal calves

Growth performance, rumen development, and sensorial meat quality of goat kids fed extruded and pelleted starters

The aim of this study was to determine the effects of supplementing extruded and pelleted starter feeds on growth performance, rumen development, carcass characteristics, and sensorial meat quality of goat kids fed an all-milk protein replacer. Forty-five new-born French Alpine kids (21 males, 4.93 ± 0.58 kg; and 24 females, 4.40 ± 0.60 kg) were randomly assigned to three treatment diets, milk replacer-only (RO), milk replacer supplemented with extruded starter feed (RE), or milk replacer supplemented with pelleted starter feed (RP) in a 3 x 2 factorial arrangement. After 35 days, the final weights, weight gain, body size measurements, organ weights, and hot and cold carcass weights were greater (p &amp;lt; 0.05) for goat kids in the RO group compared to those in the RE and RP groups. Milk replacer intake was reduced (p = 0.000) with starter supplementation, and intake of the extruded starter was higher (p = 0.001) than that of the pelleted starter. The rumen weight was greater (p = 0.019) in the RE group than in the RO group. Rumen pH was higher (p = 0.006) and acetic acid (p = 0.008) and total VFA (p = 0.012) concentrations were lower in the RO group than in the RE and RP groups. Mesenteric fat content was higher (p = 0.002) in the RO group than in the other groups. Meat odor of the RO group was preferred (p = 0.051) by the sensorial panel. Feeding an RO diet is beneficial for goat kids sold to restaurants. For replacement kids, an extruded starter feed may improve rumen development and facilitate adaptation to post-weaning dry feed consumption.

Risk ranking of mycotoxins in plant-based meat and dairy alternatives under protein transition scenarios

While reducing the consumption of animal-source foods is recommended for planetary and human health, potential emerging food safety risks associated with the transition to dietary patterns featuring plant-based meat (PBMA) and dairy alternatives (PBDA) remain unexplored. We assessed the exposure to mycotoxins and ranked the associated health risks related to the consumption of PBMA and PBDA. We simulated diets by replacing animal-source proteins with their plant-based alternatives. A risk ratio method, based on the hazard quotient (HQ), was applied to rank mycotoxin-related food safety risks. An aggregated dataset containing contamination data of 45 mycotoxins distributed over 182 PBDA and 131 PBMA samples, collected and analyzed in Europe, representing a convenience sample, was used as input in our risk assessment. The highest risk mycotoxins identified for PBMA in the lower bound scenario (LB) were the sum of aflatoxins (sum AF), alternariol monomethyl ether (AME), aflatoxin B1 (AFB1) and alternariol (AOH), with HQs for children of 121.3, 66.8, 24.0 and 4.3, respectively. In the milk substitution model (LB), HQs of 4.9 (sum AF) and 1.4 (AFB1) were calculated for children, indicating a potential food safety risk. Further analysis demonstrated that soy-based meat alternatives are the highest risk food products, in particular for sum AF, AME, AOH and AFB1. With regard to PBDA, highest HQs were found for sum AF in almond and oat drink, and AFB1 in oat drink. This research presents an approach to overcome the lack of data in the assessment of emerging risks associated with the shift to more sustainable dietary patterns.

Effects of amount of lactose in milk replacer on gastrointestinal function of dairy calves

The objective of this study was to evaluate the effects of feeding milk replacer (MR) at different lactose amount while keeping osmolality constant on gastrointestinal function, blood parameters, and inflammation-related mRNA expression in the livers of dairy calves. Fifteen Holstein bull calves were assigned to one of three dietary treatments differing in MR lactose content (L: 38 %, M: 41 %, and H: 46 %). Feeding of the test diets was started at 1 day of age and gradually increased to a maximum feeding rate at 20 days of age (L: 1.16 kg/d, M: 1.21 kg/d, and H: 1.26 kg/d DM). Under these conditions, the lactose dosages for the treatments were 441 g/d, 496 g/d, and 580 g/d, respectively. The MR were prepared to ensure isocaloric and iso-osmotic (451 mOsm/kg) conditions. Fecal scores were recorded daily, and at 14 and 28 days of age, blood and breath samples were collected before and after MR feeding. In addition, feces and urine were collected for 2 consecutive days. Then, the calves were slaughtered to evaluate intestinal permeability and liver mRNA expression. The permeability in the duodenum and ileum was lower in H and M than in L; the permeability in the jejunum was also lower in H than in L. The hepatic mRNA expressions of toll-like receptor-2, IL-1β, and tumor-necrosis factor-α were lower in H and M than L. Nitrogen retention was higher in H than in L, and linear and quadratic increasing trends were observed in tissue ratio of gastrointestinal tract with the increase in lactose amount. Postprandial increase in plasma glucose concentration was smaller and postprandial increase in TG concentration was higher in H than in L. Fecal properties, digestibility, and breath hydrogen concentrations were not affected by treatment. These results indicate that feeding high-lactose MR may increase gastrointestinal weight and decrease permeability in the small intestine of calves.

Isolation of an exopolysaccharide-producing Weissella confusa strain from lettuce and exploring its application as a texture modifying adjunct culture in a soy milk alternative

Consumers often seek healthier options but still desire the familiar eating experience of traditional dairy. Incorporating exopolysaccharide (EPS)-producing cultures into fermented plant-based milk alternatives (PBMAs) offers a promising approach to improving the textural quality of these products. For this, it is essential that the EPS-producing cultures are able to produce EPS in the plant-based substrate. The present study screened 593 plant-derived lactic acid bacteria (LAB) for their ability to produce EPS on a soy milk agar medium. Fifteen LAB isolates (eight Weissella spp. and seven Leuconostoc spp.) exhibited high EPS production. One of the strongest EPS producers was a Weissella confusa strain, and genome sequencing revealed the presence of two potential related EPS genes. To identify the key gene responsible for EPS production in soy milk, 70,000 colonies were screened on soy milk agar and isolated a spontaneous EPS-defective mutant. The mutant (W. confusa dsr1) had a mutation in a putative dextransucrase gene, which could encode the enzyme catalysing the transfer of glucose from sucrose into a growing chain of dextran. The mutation introduced a premature stop codon, disrupting the enzyme production. Another mutant (W. confusa sac) found during this screen had impaired acidification and growth in soy milk, which was linked to a mutation in the sucrose metabolism gene cluster. Soy milk fermentations using the W. confusa wild-type or sac mutant, significantly increased water holding capacity and viscosity. This suggests their potential to enhance EPS production in fermented PBMAs, bringing their texture closer to that of traditional dairy.

Effect of Oat Hay as a Substitute for Alfalfa Hay on the Gut Microbiome and Metabolites of Yak Calves.

To evaluate the impact of different roughages on the intestinal microbiota of yak calves, we fed them oat hay in substitution of alfalfa hay, in addition to milk replacer and starter powder. Twenty-one 45-day-old male yak calves were selected and randomly assigned to three groups: the milk replacer + starter + alfalfa hay group (AH), the milk replacer + starter + oat hay group (OH), and the milk replacer + starter + mixed hay group (AO), in which the alfalfa hay and oat hay were administered in a 1:1 ratio. All calves in the three groups were fed the same milk replacer and an equivalent amount of dry matter. The formal experiment commenced after a 21-day pre-test period and lasted for 120 days. Following the experiment, the contents of the jejunum and colon were collected to investigate the intestinal microbiota and metabolites using 16S rRNA sequencing and LC-MS metabolomics. The result showed that the AO group had greater final body weights overall than the AH group and OH group (p < 0.05). The AH group and OH group had considerably greater feed-to-gain ratios than the AO group (p < 0.05). At the phylum level, the OH group exhibited an increased relative abundance of Bacteroidota and Spirochaetota in the jejunum (p < 0.05). The relative abundance of Actinobacteriota in the colon was increased in the AO group (p < 0.05). At the genus level, the AO group exhibited a decreased abundance of Clostridium sensu_stricto_1 (p < 0.05), and the OH group showed an increased abundance UCG-005 and Alistipes in the jejunum. There were many differential metabolites in the OH group and AO group compared to the AH group, and the different metabolites of the OH group were associated with the metabolic pathways of the nervous system, sensory system, amino acid metabolism, and lipid metabolism in the jejunum and with lipid metabolism, amino acid metabolism, and the nervous system in the colon. In the AO group, these metabolites were associated with the digestive system and the translation and metabolism of cofactors in the jejunum and with the metabolism of cofactors and vitamins in the colon. In summary, it is feasible to replace alfalfa hay with oat hay based on milk replacer and starter. The combination of the two forages enhanced nutrient absorption, improved immune function, maintained the internal homeostasis of yak calves, and was more beneficial to their growth and development.

The role of dairy alternatives in just food system transitions: a scoping review

AbstractAlternatives to dairy products are becoming part of mainstream food culture in Western societies amidst growing concerns for more sustainable and healthy diets, and improved animal welfare. As more citizens opt for plant-based milks, markets have responded with an abundance of alternatives to facilitate the transition. Previous systematic reviews have examined the environmental and health impacts of plant-based products compared with dairy, yet a synthesis of empirical research on the social and environmental justice impacts of dairy alternatives is lacking. This systematic scoping review aims to explore the extent, range, and nature of peer-reviewed studies on dairy alternatives that address their outcomes for justice. Publications were sought through systematic search of two comprehensive electronic databases (Scopus and Web of Science) and screened by two independent reviewers following eligibility criteria. Included studies predominantly focused on consumer identities and values, and the market-led nature of dairy alternatives consumption. Gaps in the literature included impacts on nutritional equity and the innovation and adaptive capabilities of food producers and workers across geographies. We conclude that the heavy-handed regulation of dairy alternative terminology and the market-driven nature of this transition raise questions about injustices in governance and innovation in this sector.

Towards hybrid protein foods: Heat- and acid-induced hybrid gels formed from micellar casein and pea protein

Given the rising demand for more sustainable, cookable dairy alternatives, this research explores the formation and characteristics of heat- and acid-induced gels combining micellar casein and pea protein. Protein dispersions (4 % w/w) of commercial micellar casein isolate and pea protein isolate were prepared and preheated (95°C, 30 min) separately before mixing in varying ratios (75:25 %, 50:50 %, and 25:75 % w/w). After emulsifying with milk fat (3.5 % w/w), the protein mixtures were heated to 80 °C and acidified to pH 5.2 (citric acid). The resultant coagula were pressed, drained, and molded to obtain the final gel. It was observed that adding pea protein led to a higher yield of coagula with more serum retained. As the proportion of pea protein increased, the total solids (TS), protein, and fat content of the gels decreased linearly. The micellar casein gel showed significantly higher hardness, elasticity, and chewiness than the gels containing pea protein. Moreover, the micellar casein gel did not show clear fracture behavior under large deformation, while the gels containing pea protein were more prone to rupture. These textural differences were explained by the changes in gel compositions, protein interactions, and gel microstructure. The composition and textural properties of hybrid gels showed a strong linear relationship with pea protein fractions, showing the possibility of customizing gel properties. Notably, the hybrid gel containing 25 % pea protein exhibited promising characteristics, closely resembling those of the commercial dairy paneer product.