Daily Weight Gain Research Articles

Effects of Different Dietary Combinations on Blood Biochemical Indicators and Rumen Microbial Ecology in Wenshan Cattle

With the continuous optimization of feed ingredients in livestock production, barley has garnered significant attention as a potential substitute for corn in feed. This study aims to investigate the effects of replacing part of the corn and soybean meal with barley, wheat bran, and rapeseed meal on Wenshan cattle, focusing on the rumen microbial community, blood physiological and biochemical indicators, and growth traits. Through an intensive feeding experiment with two different dietary ratios, we found that adding barley to the diet significantly reduced the host’s blood lipid concentration and significantly increased the height, body length, heart girth, and average daily weight gain of Wenshan cattle. Analysis of the rumen microbial community structure showed that the addition of barley significantly affected the relative abundance of Firmicutes, Proteobacteria, and Bacteroidetes, with the relative abundance of Spirochaetes being significantly lower than that of the control group (p < 0.05). The dominant bacterial groups mainly included Acinetobacter, Solibacillus, and Lysinibacillus. In summary, this study reveals the potential of different feed ingredient ratios involving barley, wheat bran, and rapeseed meal in the production performance of Wenshan cattle. By regulating blood physiology and improving the rumen micro-ecological structure, it provides new scientific evidence for optimizing livestock and poultry feeding management strategies. Future research will further explore the optimal application ratio of barley under different feeding conditions and its long-term impact on animal health and production performance.

Alteration of Cecal Microbiota by Antimicrobial Peptides Enhances the Rational and Efficient Utilization of Nutrients in Holstein Bulls.

We previously observed that supplementation with antimicrobial peptides facilitated the average daily weight gain, net meat, and carcass weights of Holstein bulls. To expand our knowledge of the possible impact of antimicrobial peptides on cecum microbiota, further investigations were conducted. In this study, 18 castrated Holstein bulls with insignificant weight differences and 10 months of age were split randomly into two groups. The control group (CK) was fed a basic diet, whereas the antimicrobial peptide group (AP) was supplemented with 8 g of antimicrobial peptides for 270 days. After slaughter, metagenomic and metabolomic sequencing analyses were performed on the cecum contents. The results showed significantly higher levels of amylase, cellulase, protease, and lipase in the CK than in the AP group (P ≤ 0.05). The levels of β-glucosidase and xylanase (P ≤ 0.05), and acetic and propionic acids (P ≤ 0.01), were considerably elevated in the AP than in the CK group. The metagenome showed variations between the two groups only at the bacterial level, and 3258 bacteria with differences were annotated. A total of 138 differential abundant genes (P < 0.05) were identified in the CAZyme map, with 65 genes more abundant in the cecum of the AP group and 48 genes more abundant in the cecum of the CK group. Metabolomic analysis identified 68 differentially expressed metabolites. Conjoint analysis of microorganisms and metabolites revealed that Lactobacillus had the greatest impact on metabolites in the AP group and Brumimicrobium in the CK group. The advantageous strains of the AP group Firmicutes bacterium CAG:110 exhibited a strong symbiotic relationship with urodeoxycholic acid and hyodeoxycholic acid. This study identified the classification characteristics, functions, metabolites, and interactions of cecal microbiota with metabolites that contribute to host growth performance. Antimicrobial peptides affect the cecal microorganisms, making the use of nutrients more efficient. The utilization of hemicellulose in the cecum of ruminants may contribute more than cellulose to their production performance.

Impact of fermented wine lees on gut microbiota and metabolic responses in Guanling crossbred cattle

BackgroundThe addition of wine lees to diets can make up for the deficiencies caused by traditional forages in beef cattle farming. However, the effects of different wine lees ratios on average daily weight, gastrointestinal microbial community structure and metabolites in Guanling crossbred cattle have been rarely studied. This study assessed the effects of feeds containing wine lees on weight gain, gastrointestinal microbial community structure, and metabolites in Guanling crossbred cattle and elucidated the metabolic responses induced by wine lees. Eighteen cows were randomly assigned to receive fed concentrate (C group), feed containing 15% wine lees (group A), or feed containing 30% wine lees (group B) for 60 days.ResultsThe average daily weight gain of group A and group B increased by 76.75% and 57.65%, respectively, compared with group C. Microbial community analysis showed that wine lees increased the abundance of Prevotella_1 in the rumen, decreased the abundance of Ruminococcaceae UCG 011 and Lachnospiraceae_FCS020_group in the rumen, and increased the abundance of Tyzzerella_4, Family_Xlll_AD3011_group, Granulicella, and Eisenbergiella in the cecum. Metabolomics analyses showed that wine lees decreased the concentrations of indole-3-ethanol in the rumen, and complexity cecal metabolism. Notably, linoleic acid metabolism was significantly enriched in both the rumen and cecum. Mantel test analyses indicated that the adverse effects of WL were reduced by stimulating the metabolism of linoleic acid, α-linolenic acid, and tryptophan, and these changes were mediated by intestinal microorganisms. The Guanling cattle cecum was enriched for several unfavorable metabolic pathways when wine lees concentrations reached 30%, which increased the likelihood of intestinal lesions.ConclusionThis study shows that WL supplementation alters gut microbiota and metabolic pathways, improving cattle growth and health. Moderate WL levels (15%) enhance gut health and beneficial pathways (e.g., linoleic and alpha-linolenic acid metabolism). However, higher WL inclusion (30%) may activate adverse pathways, raising the risk of intestinal damage. To maximize benefits and minimize risks, WL levels should be carefully managed.

Biological Mechanisms of Aflatoxin B1-Induced Bile Metabolism Abnormalities in Ducklings.

This study investigated the effects and biological mechanisms of aflatoxin B1 (AFB1) on the health and bile metabolism of ducklings. Forty-eight 1-day-old ducklings were randomly assigned to two groups, with six replicates per group. The control group was fed a basic diet, while the AFB1 group received a diet containing 90 µg/kg of AFB1. The experiment lasted for 2 weeks. The results showed that 90 µg/kg AFB1 caused abnormal bile metabolism; damaged liver cell nuclei and mitochondria; and significantly decreased body weight, average daily weight gain, and levels of albumin, total protein, cholesterol, total superoxide dismutase, glutathione peroxidase, and glutathione. It also significantly increased feed conversion efficiency, along with alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bile acids, and malondialdehyde levels. In the liver, the expression levels of CYP7A1, SCD, and other genes were significantly upregulated, while BSEP, FASN, HMGCR, CAT, and other genes were significantly downregulated. In conclusion, AFB1 causes abnormal bile metabolism and impairs the overall health and liver function of ducklings. Its mechanism of action may involve changes in gene expression related to bile acid metabolism, lipid metabolism, oxidative damage, and cancer pathways.

Adding red propolis to the diet of Lacaune lambs: Effects on animal health, ruminal environment, performance, and meat quality

This study determined the influence of red propolis ethanolic extract in lambs' diets on the ruminal environment, animal health, and growth. Two experiments were carried out to achieve these objectives. Pilot study: Twenty-four female Lacaune lambs were divided into four groups, with three replicates and two animals per replicate (pen): T0 (Control), TP1.3, TP2.7, and TP5.3, representing 0, 1.3, 2.7, and 5.3 mL of propolis extract per animal/day, respectively. The highest daily weight gain was observed in lambs that consumed 2.7 mL of propolis extract per day, and they also had the lowest feed conversion rate compared to the control. Lower total leukocyte counts were observed in lambs that consumed propolis extract compared to control due to lower granulocyte and lymphocyte counts. Ceruloplasmin and C-reactive protein levels were lower in the serum of lambs that consumed propolis compared to the control. Lambs that consumed 1.3 and 2.7 mL of propolis extract had lower TBARS levels than the control. The effect of treatment was observed for ruminal pH, which was higher in lambs that consumed propolis extract than in control. A lower concentration of short-chain fatty acids was observed in lambs that consumed propolis than the control due to lower concentrations of acetic and butyric acid. In experiment II, 36 male Lacaune lambs were selected and divided into two groups: MONENSIN (23.45 ppm of monensin per kg/dry matter (DM)) and PROPOLIS (3.8 mL of propolis per animal/day). The animals that consumed propolis extract had greater body weight, weight gain, and average daily gain. These also had a shorter time to reduce methylene blue, thus demonstrating more significant bacterial activity in the rumen. Furthermore, they had lower counts of Escherichia coli, total coliforms, and Enterobacteria. The meat's fatty acid profile in animals fed the red propolis extract showed higher concentrations of monounsaturated fatty acids. In conclusion, adding red propolis extract to the concentrate increased weight gain, average daily gain, and oxidative balance with an antimicrobial and anti-inflammatory effect.

Influence of probiotic strains on growth performance, carcass characteristics and seasonal changes of growing New Zealand white rabbits.

This experiment was designed to explore how different types of probiotics affect the growth, carcass traits, and seasonal variations in growing New Zealand White rabbits (NZW). Two parallel experiments using the same strain of NZW during winter and summer, each alone from 5 to 13 weeks of age. Each experiment uses a total of 125 unsexed rabbits. These rabbits are separated into 5 groups of 25 rabbits each. Each group has five replicates, with five rabbits in every replicate. In each experiment, 1st group acting as the control group did not receive any probiotics. The 2nd was given a dose of 1ml of Bifidobacterium bifidum, the 3rd received a dose of 1ml of Lactobacillus acidophilus, and the 4th was treated with a 1ml blend of both Bifidobacterium bifidum and Lactobacillus acidophilus, and 5th group was treated with 1ml of Saccharomyces cerevisiae. Results indicated that the Bifidobacterium bifidum group had the best live body weight (LBW) values and daily weight gain (DWG). Meanwhile, during summer, the Lactobacillus acidophilus group had the best feed conversion ratio (FCR) and performance index (PI) values. Also, growing rabbits fed Lactobacillus acidophilus and Bifidobacterium bifiduim had significantly increased carcass traits during the summer and winter seasons. Furthermore, seasonal changes indicated that the Bifidobacterium bifiduim group improved LBW, DWG, and PI during summer than winter. So, it could be concluded that using Bifidobacterium bifidum can enhance rabbit growth by improving feed utilization and carcass traits, making it an effective addition to hot weather diets.

Effects of low energy diets supplemented with emulsifier on growth performance, nutrient digestibility, and intestinal morphology of broiler chickens

This study was conducted to investigate the effects of adding an emulsifier to energy-deficient diets on growth performance, intestinal morphology, and nutrient digestibility in broiler chickens. A total of 540 one-day-old male broiler chickens were randomly assigned to a 3 × 3 factorial arrangement of treatments with 4 pens and 15 broiler chickens/pen for 6 wk. Nine treatments consisted of 3 inclusion rates of emulsifier (0, 250, and 500 mg kg-1) and the standard, moderate, and low energy concentrations [45 and 90, 60 and 105, and 75 and 120 kcal apparent metabolizable energy (AME)/kg less for the moderate- and low-energy concentrations than the standard energy requirements during the starter (d 0 to 10), grower (d 10 to 24), and finisher (d 24 to 42) phases, respectively]. The average daily weight gain (ADWG) and average daily feed intake (ADFI) were recorded in the starter, grower, finisher, and total rearing (d 0 to 42) phases and feed conversion ratio (FCR) was calculated. The AME, digestibility of crude protein (CP), lipid, and organic matter (OM) of experimental diets, were determined from d 5 to 10 and from d 35 to 40. Broiler chickens fed moderate- or low-energy diets containing emulsifier showed greater ADWG and LBW, than those fed the standard diets along with lower FCR, compared to the same diets without emulsifier (P < 0.05). Live body weight increased, while both ADFI and FCR decreased linearly and quadratically with an increase in emulsifier inclusion rate in the diets (P < 0.05). Broiler chickens fed moderate-energy diets with 500 mg emulsifier /kg or low-energy diets containing 250 mg emulsifier /kg had greater villus height compared to those fed other diets (P < 0.05). The addition of emulsifiers to diets led to a linear increase in villus height, epithelium height, and the villus height to crypt depth ratio (P < 0.05). In moderate- and low-energy diets, the inclusion of emulsifiers resulted in greater AME and digestibility of lipid and OM (P < 0.05). The AME and digestibility of lipid and OM increased linearly and quadratically with an increase in emulsifier inclusion rate in the diets (P < 0.05). In conclusion, the addition of emulsifier at a rate at least 250 mg kg-1 to moderate- or low-energy diets during the starter, growth, and finisher phases can enhance broiler chicken performance by improving the histological characteristics of small intestine and enhancing lipid digestibility.

Canopy Characteristics of Gamba Grass Cultivars and Their Effects on the Weight Gain of Beef Cattle under Grazing

Gamba grass (Andropogon gayanus Kunth) is a tussock-forming forage species adapted to acid soils of Brazilian savannas and cultivated for grazing pastures. Four decades since its release, Planaltina prevails as the most commercialized cultivar of the species, even though the new cultivar BRS Sarandi could be a better alternative for Gamba-grass-based farms by presenting a greater leaf:stem ratio. The objective of this study was to evaluate the average daily live weight gain (ADG) of Nellore bulls (Bos indicus) for two Gamba grass cultivars—Planaltina and Sarandi. The experiment was conducted in Planaltina, Federal District, Brazil, for 3 years, namely 2018, 2018–2019, and 2020. The experimental design was a completely randomized block design with two treatments and three replicates, each one continuously stocked at three stocking rates (SR)—1.3, 2.6, and 4 young bulls/ha. Canopy height (CH), forage mass (FM), plant-part proportion (green leaf, stem, and dead material), and nutritive value were evaluated. In 2018, mean ADG for Sarandi pastures was greater (0.690 kg/bull/d) than that of Planaltina (0.490 kg/bull/d) (p &lt; 0.10). In the subsequent year (2018–2019), there was no effect of cultivar (p &gt; 0.10), while in 2020 the ADG was again affected by cultivar (p &lt; 0.10), confirming the advantage of Sarandi (0.790 kg/bull/d) over Planaltina (0.650 kg/bull/d). In 2018 and 2020, the percentage of stems for Sarandi was about 3–6 pp less than for Planaltina (p &lt; 0.10). As well as for stems, Sarandi pastures presented a shorter CH in 2028 and 2020 (6–7%) (p &lt; 0.10). The positive high correlation of leaf:stem ratio with ADG (r = 0.70) probably predisposed the superiority of Sarandi over Planaltina. The distinguishing plant-part composition of Sarandi canopy promotes increasing weight gain of beef cattle when compared to cv. Planaltina.

Residual feed intake and gain alters the performance and carcass traits in Nellore cattle.

The residual intake and gain (RIG) aims to select animals that present low feed intake in relation to the expected. This study aimed to evaluate the associations of selection for RIG with performance and carcass traits in Nellore cattle. Initially, residual feed intake (RFI) and residual gain (RG) were determined. From this, the RIG was calculated, and the animals were classified as efficient and inefficient for RIG. The efficient animals for RIG showed higher daily weight gain (DWG), Longissimus muscle area (LMA), and mid-test metabolic body weight (MMBW) than the inefficient ones. No significant correlations were found between subcutaneous fat thickness (SFT), marbling (MAR), LMA, MMBW, and the RIG. Thus, it's concluded that the RIG is a measure that can be used to identify and select animals with higher rates of DWG and LMA without changes in the dry matter intake (DMI), SFT, and MAR. However, this index should still be observed with caution, as it may be dependent on body size. Based on the findings, the selection of animals according to the RIG can be an important factor to generate phenotypic evolution in characteristics such as weight gain and rib eye area without adverse effects on the carcass fat deposition.

Performance, Meat Quality and Gene Expression of Grazing Lambs Supplemented with Macadamia Oil and Vitamin E

Macadamia oil has high concentrations of oleic and palmitoleic fatty acids, which can increase tissue sensitivity to insulin, improving glucose absorption efficiency, and reducing lipogenesis through gene modulation. The objective of this study was to evaluate the effects of macadamia oil associated with vitamin E supplementation on performance, blood parameters, meat quality and sensory characteristics, meat fatty acid profile, and expression of genes associated with lipid metabolism in grazing lambs. The experimental treatments were control diet (Control), Control + 0.1% of body weight of macadamia oil (MO), MO + 745 IU of vitamin E/dry matter (MOVE). Macadamia oil improved feed efficiency, reflecting a lower dry matter intake, as the average daily weight gain did not differ from Control. Meat quality parameters were not affected by macadamia oil or vitamin E supplementation. Supplementation with macadamia oil improved meat appearance, flavor, and overall liking. Supplementation with macadamia oil provided a higher proportion of C18:3 n3 and a lower proportion of CLA. The expression of SREBP-1c, PPAR-α, SCD1, and ELOVL6 genes were not modified with the supplementation of macadamia oil and vitamin E. In conclusion, supplementation with macadamia oil improves feed efficiency and meat quality; and the inclusion of 745 IU of vitamin E/kg of dry matter for grazing lambs reduces 36% of lipid oxidation of the meat.

Effects of including different levels of equal mix of soybean and flaxseed oils in Japanese quail diets on the growth, carcass quality, and blood biomarkers

Corn, the primary ingredient in modern poultry feeds, contains high levels of ω-6 fatty acids but lacks sufficient ω-3 fatty acids, creating an imbalance. Maintaining a balance between ω-6 and ω-3 fatty acids in poultry diets is crucial due to their competition. This study aimed to evaluate the effects of incorporating different concentrations of an equal mix of soybean oil (SO) and flaxseed oil (FO) into quail diets on growth performance, carcass quality, and blood biochemistry. One-week-old Japanese quail birds (n=200) were randomly assigned to four dietary groups, each comprising five replicates with 10 chicks per replicate. Four isonitrogenous/isocaloric basal diets were formulated. Group 1 (control) received a basal diet without SO or FO, while Groups 2-4 received basal diets supplemented with an equal mix of SO+FO at levels of 1.0%, 1.5%, and 2.0%, respectively. The inclusion of oil mixes significantly increased body weight (BW) at five weeks and daily weight gain (DWG) during weeks 3-5 and 1-5. The feed conversion ratio (FCR) improved with the addition of oil mixes throughout the trial period. Supplementing quail diets with oil mixes resulted in reduced serum total cholesterol (TC) and LDL cholesterol, elevated serum HDL cholesterol, and no significant effect on triglycerides (TG) and VLDL cholesterol levels. Quails fed oil-supplemented diets showed lower serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and creatinine levels, while urea and uric acid were significantly affected. Birds fed diets with oil mixes also had increased serum concentrations of immunoglobulin G (IgY), immunoglobulin M (IgM), and superoxide dismutase (SOD). However, serum immunoglobulin A (IgA), malondialdehyde (MDA), and total antioxidant capacity (TAC) levels did not significantly change across experimental groups. Overall, adding up to 2% of the SO and FO mix in growing quail feeds improved growth performance, blood lipid profile, liver and kidney function markers, immune response, and antioxidant defense. The highest level of oil mix (2%) yielded the most beneficial effects.

Effect of supplementation of cactus (Opuntia ficus-indica) cladodes, Acacia saligna, wheat bran and cotton seed cake on feed intake, digestibility, growth and carcass characteristics of goats

The objective of this study was to evaluate the effect of supplementation of cactus (Opuntia ficus-indica) cladodes, Acacia saligna, wheat bran and cotton seed cake on growth, digestibility, intake and carcass characteristics of goats. A randomized complete block design was used in the experiment with 24 yearling central highland goats with an initial body weight of 15.6 - 16.1kg. The same amount of grass hay (GH) + 150 gDM/head/day wheat bran (WB) was given to all animals. The experimental diets consisted of 80 cotton seed cake (CSC) as treatment 1 (T1), 45CSC +160 cactus cladodes (CC) as T2, 45CSC+ 80 Acacia saligna (AS) as T3, and 45CSC+80CC+40AS as T4 (gram dry matter: DM, per day per goat), Data were gathered on the goats' growth, digestibility, intake, and carcass of major organs, edible and nonedible organs. The consumption of dry matter and organic matter was higher in goats fed T2 and T4 than in the T1 group. The DM, organic matter (OM) and crude protein (CP) digestibility, average daily body weight gain and feed conversion efficiency were higher in T4 and T3 goats when compared to T2 goats. Goats fed on T4 had higher hot carcass weight and dressing percentage on slaughter body weight basis than T2, T3, and T1 supplemented goats. Generally, the experimental diets improved goats’ performance in descending order (T4 &gt; T3 &gt;T1 &gt;T2). Supplementation of T4 (replacement of 35 g DM of cotton seed cake per day by 40g of Acacia saligna and 80g of cactus cladodes on dry matter bases) could be recommended to improve goat performance.

Performance and Physiological Responses of Pre-Weaning Peranakan Etawa Goats Fed Milk Replacer with Maggot Flour

Pre-weaning growth is crucial for achieving high weaning weights, which are expected to positively correlate with the overall performance of does/ewes. Milk replacer, designed to meet the nutritional needs of pre-weaning kids, offers a solution to this challenge. This study aimed to analyze and evaluate the impact of incorporating maggot flour into milk replacer on the performance of pre-weaned Peranakan Etawa (PE) crossbreed goats. The parameters observed included milk replacer intake, nutrient consumption, body weight gain, weaning weight, rectal temperature, respiratory rate, and feed cost per gain. The study involved 10 two-week-old local pre-weaned PE goats, each with an average body weight of 5.33 ± 0.93 kg, divided into two treatment groups with five replicates each. The treatments included a commercial milk replacer (P0) and a milk replacer containing maggot flour (P1). Data were analyzed using an independent t-test at a 5% significance level. The results showed that daily body weight gain in pre-weaned PE goats fed milk replacer containing maggot flour did not differ significantly (P0.05) from those fed the commercial milk replacer. The physiological responses of the pre-weaned goats, including rectal temperature and respiratory rate, were similar between treatments and did not negatively affect their physiological status. The lowest feed cost per gain was observed in the group fed the maggot flour-based milk replacer (P1), indicating its potential economic advantage.

Investigating the chemical composition, digestion and fermentation of Alternanthera sessilis red plant and its effect on the performance of fattening lambs.

The present experiment was conducted to evaluate the nutritional value of red Alternanthera sessilis for fattening lambs when they were replaced with alfalfa forage. Forty growing lambs with an average weight of 21.12kg and an age of 5 months were randomly assigned to four experimental treatments. Growth performance, nutrient digestibility, rumen fermentation parameters, protozoa population, blood parameters, and composition of carcass components were evaluated. The results of this experiment showed that the use of Alternanthera sessilis in the diet significantly reduced feed intake, increased the average daily weight gain, and improved the feed conversion ratio compared to the control treatment (P < 0.05). The digestibility of dry matter and protein was significantly increased (P < 0.05), while the digestibility of neutral detergent fiber, acid detergent fiber, and organic matter showed a numerical increase. Diets containing different levels of the Alternanthera sessilis plant did not affect pH, but the increase in the amount of this plant in the diet led to an increase ammonia nitrogen concentration and rumen protozoa population (P < 0.05). The addition of Alternanthera sessilis to the diet significantly reduced the concentration of blood glucose and cholesterol and increased the concentration of blood urea nitrogen (P < 0.05). Except for the weight of the thigh and neck, the effect of experimental treatments on other carcass components was not significant. Overall, the results of this experiment showed that using the Alternanthera sessilis plant in the rations of fattened lambs as a substitute for alfalfa forage not only had no negative effect on the studied parameters but also improved them in some cases. Therefore, Alternanthera sessilis can be used in rations of fattened lambs as a substitute for part of alfalfa forage.

The Impact of Turmeric, Curcuma, and Ginger Feed Additives on the Productivity of Kaur Cattle as a Genetic Resource in Bengkulu

Kaur cattle, a local breed in Bengkulu, represent a valuable genetic resource that requires preservation and effective utilization to enhance livestock production. Turmeric, curcuma, and ginger are plants known for their essential oils and curcumin content, which offer numerous proven benefits. This study aimed to evaluate the impact of these feed additives on the productivity of Kaur cattle. A quantitative research method employing a completely randomized design was used, consisting of four treatments and four replications. The diet comprised wild grass (10% of body weight) and palm kernel meal in an 80:20 ratio. The treatments were as follows: P0 (control: wild grass and palm kernel meal), P1 (wild grass, palm kernel meal, and turmeric powder Curcuma xanthorrhiza Roxb.), P2 (wild grass, palm kernel meal, and curcuma powder Curcuma longa L.), and P3 (wild grass, palm kernel meal, and ginger powder Zingiber officinale Rosc.). The study involved 16 male Kaur cattle aged 1.5-2 years, with an average body weight of 160-180 kg, observed over 60 days. The variables measured included average daily weight gain, forage consumption, total feed consumption, feed efficiency, and cost efficiency. Data were analyzed using a one-way ANOVA. The results showed that the mean daily weight gain (kg/head/day) was P0=0.35±2.57, P1=0.47±2.39, P2=0.50±2.46, and P3=0.49±2.88, with no significant differences between treatments. The mean feed efficiency (%) was P0=0.12±4.44, P1=0.25±4.71, P2=0.27±5.22, and P3=0.26±4.38, also showing no significant differences. The mean cost efficiency for feed use was P0=35,869±7.47, P1=34,281±7.08, P2=33,220±7.39, and P3=35,410±7.21, with no significant differences observed. Overall, the addition of turmeric, curcuma, and ginger powders to the feed did not significantly impact the productivity of Kaur cattle.

Hepatic transcriptomic analysis reveals differential regulation of metabolic and immune pathways in three strains of chickens with distinct growth rates exposed to mixed parasite infections.

During parasite infections, the liver may prioritise immune-related pathways over its metabolic functions. Intestinal infections caused by Ascaridia galli and Heterakis gallinarum impair feed intake, nutrient absorption, and weight gain. Histomonas meleagridis, vectored by H. gallinarum, can also damage liver tissues, potentially impairing liver functions. This study examined the hepatic gene expression in three strains of chickens: Ross-308 (R), Lohmann Brown Plus (LB), and Lohmann Dual (LD), 2weeks after an experimental infection (n = 18) with both A. galli and H. gallinarum or kept as uninfected control (n = 12). Furthermore, H. gallinarum infection led to a co-infection with H. meleagridis. The mixed infections reduced feed intake and the average daily weight gain (P < 0.001). The infections also increased the plasma concentrations of alpha (1)-acid glycoprotein and the antibody titre against H. meleagridis (P = 0.049), with no strain differences (P > 0.05). For host molecular response, 1887 genes were differentially expressed in LD, while 275 and 25 genes were differentially expressed in R and LB, respectively. The up-regulated genes in R and LD were mostly related to inflammatory and adaptive immune responses, while down-regulated genes in LD were involved in metabolic pathways, including gluconeogenesis. Despite performance differences among the strains, worm burdens were similar, but hepatic molecular responses differed significantly. Moreover, there was an indication of a shift in hepatic functions towards immune-related pathways. We, therefore, conclude that the liver shifts its functions from metabolic to immune-related activities in chickens when challenged with mixed parasite species.

Trajectories of daily antipsychotic use and weight gain in people hospitalized for the first episode of psychosis.

We need to better understand the risk factors and predictors of medication-related weight gain to improve metabolic health of individuals with schizophrenia. This study explores how trajectories of antipsychotic medication (AP) use impact body weight early in the course of schizophrenia. We recruited 92 participants with first-episode psychosis (FEP, n=92) during their first psychiatric hospitalization. We prospectively collected weight, body mass index (BMI), metabolic markers, and exact daily medication exposure during 6-week hospitalization. We quantified the trajectory of AP medication changes and AP polypharmacy using a novel approach based on meta-analytical ranking of medications and tested it as a predictor of weight gain together with traditional risk factors. Most people started treatment with risperidone (n=57), followed by olanzapine (n=29). Then, 48% of individuals remained on their first prescribed medication, while 33% of people remained on monotherapy. Almost half of the individuals (39/92) experienced escalation of medications, mostly switch to AP polypharmacy (90%). Only baseline BMI was a predictor of BMI change. Individuals in the top tercile of weight gain, compared to those in the bottom tercile, showed lower follow-up symptoms, a trend for longer prehospitalization antipsychotic treatment, and greater exposure to metabolically problematic medications. Early in the course of illness, during inpatient treatment, baseline BMI is the strongest and earliest predictor of weight gain on APs and is a better predictor than type of medication, polypharmacy, or medication switches. Baseline BMI predicted weight change over a period of weeks, when other traditional predictors demonstrated a much smaller effect.

Comparative study of mash vs pellet feed on growth performance and feed utilization by goat

This study was conducted in Bharatpur-11, Chitwan, Nepal, to evaluate the effects of two feed forms (mash and pellet) on the growth performance and feed utilization in goats. Twenty Boer cross female goats, averaging five months in age, were assigned to either mash or pellet feed treatments, with 10 goats in each group, for a 90-day period, with two weeks of adaptation. The experimental animals were given concentrate feed at 1% of their body weight daily, along with ad libitum access to Napier grass and clean drinking water. Both feed types had a dry matter content of 90% and a crude protein level of 17%. Key parameters assessed included feed and forage intake, body weight changes, and feed wastage. Goats on pelleted feed showed a significantly higher average daily weight gain of 62.00 g/day compared to 26.11 g/day for those on mash feed. Final body weights were also greater in the pellet-fed group (28.88 kg) compared to the mash-fed group (25.04 kg). Additionally, pelleted feed led to higher intake of concentrate and forages and significantly reduced feed wastage, with pellet feed waste being 1.22 g/day versus 27.65 g/day for mash feed. These findings indicate that pelleted feed enhances growth performance, feed intake, and feed efficiency in goats. Therefore, this study indicated that pelleted feed is a more effective option for enhancing goat productivity and reducing feed wastage.

Optimizing feeding frequencies in fish: A meta-analysis and machine learning approach

Feeding is a critical factor in aquaculture, directly impacting cultivation success and profitability. Feeding frequency determines feed intake and regulates fish appetite and nutrient utilization. However, the effects of increased feeding frequency on fish growth and feed conversion ratio (FCR) can vary depending on species, cultivation conditions, feeding rates. This variability can lead to inconsistencies in interpretations, with individual studies often lacking in statistical power. To address this issue, we analyzed data from 145 study cases across 53 publications to quantify the impact of feeding frequency on average daily weight gain (ADG) and FCR. Our meta-analysis revealed that increasing feeding frequency could reduce FCR, but it did not enhance ADG when daily feed amounts were fixed. Only when each feeding reached satiation did increased feeding frequency boost ADG. We found that the relationship between feeding frequency and ADG in fish is quadratic, with most fish species achieving optimal ADG at four feedings per day. Traditional approaches suggest that fish appetite recovery is linked to gastric evacuation time, defining an optimal feeding interval at 80 % gastric evacuation. However, our research indicates that this approach is not suitable for all fish species. Therefore, we developed a Gradient Boosting Machine (GBM) model that predicts FCR (R2 = 0.9303) and ADG (R2 = 0.7249) based on environmental variables, fish characteristics, culture period, and feeding frequency. This study provides a scientific basis for formulating more efficient and sustainable aquaculture feeding strategies, optimizing growth and feed utilization across different fish species.

Blood Parameter Response in Growing Alpine Goat Kids Fed Diets Containing Extruded Flaxseed or Pumpkin Seed Cake

Blood parameters can provide information on the nutritional status of goat kids, which is related to both health and performance. The present study aimed to research whether feeding extruded flaxseed (FS) and pumpkin seed cake (PC), as an alternative protein source in diets, has an effect on the hematological and serum biochemical parameters of goat kids during growth. In the small-scale goat farm, 31 French Alpine goat kids aged 32 days were used for the study. The goat kids were subjected to three different feeding treatments: a mixture containing soybean meal and extruded soybeans (CON), a mixture containing 16% PC (PC-16), and a mixture containing 9% FS (FS-9). They were monitored during the suckling, weaning, and post-weaning growth periods. PC-16 and FS-9 in goat kids’ diets did not result in any changes regarding average daily weight gain. The WBC count was higher in goat kids fed FS-9 and PC-16 compared to CON (9.84 and 9.54 vs. 6.61 × 109 L) diets during the weaning period. GGT activity was lowest in the serum of goat kids fed PC-16 compared to CON post-weaning (38.65 vs. 48.40 U/L). In addition, FS-19 increased GPx compared to kids fed PC-16 post-weaning (809.7 vs. 600.8 U/L). Regarding blood parameters, PC-16 and FS-9 can be used in goat kids’ nutrition as alternative sources of proteins on a small-scale goat farm without compromising goat kids’ growth.