Daily Intake Research Articles

Comparison of High n-3 PUFA Levels and Cyclic Heat Stress Effects on Carcass Characteristics, Meat Quality, and Oxidative Stability of Breast Meat of Broilers Fed Low- and High-Antioxidant Diets

This study investigated the individual and combined effects of a high dietary n-3 PUFA intake and cyclic heat stress (HS) on the carcass characteristics, meat quality, and oxidative stability of broiler breast meat and the potential of antioxidant supplementation (vitamins E, C, and selenium) to mitigate these effects. A total of 192 one-day-old male Ross 308 broilers were randomly assigned to 24 pens within two controlled environment chambers and fed with the following diets: a basal diet low in antioxidants according to NRC recommendations (NRC group), a basal diet according to Aviagen recommendations additionally supplemented with 200 IU/kg vitamin E, 250 mg/kg vitamin C, and 0.15 mg/kg selenium (HAOX group), and these two diets further supplemented with 5% linseed oil (NRC N-3 and HAOX N-3 groups). On day 22, the broilers were exposed to the following two environmental conditions: thermoneutral (TN, 21 °C) or cyclic HS (HS, 34 ± 1 °C, 7 h/d) in a 2 × 2 × 2 factorial design. A high intake of n-3 PUFAs significantly decreased growth performance, dressing percentage, and breast yield, while the incidence of pale, soft, and exudative (PSE) meat characteristics and malondialdehyde (MDA) levels increased. Cyclic HS reduced body weight (BW) and average daily feed intake (ADFI), but had limited effects on meat quality. No interactions between n-3 PUFAs and HS were observed for any measurements. High antioxidant supplementation increased breast yield, improved meat quality, and reduced oxidative stress, as evidenced by an enhanced antioxidant activity and lower MDA levels. In conclusion, n-3 PUFAs had a negative effect on both the carcass characteristics and meat quality of broilers, while HS primarily affected only carcass characteristics, with neither stressor having severe adverse effects. High levels of antioxidants could mitigate the negative effects of dietary- and heat-induced oxidative stress by enhancing the oxidative stability of broiler meat.

Ciprofloxacin Concentrations in Food Could Select for Quinolone Resistance in Klebsiella pneumoniae: An In Vivo Study in Galleria mellonella

Background: The use of antimicrobials to treat food animals leaves antimicrobial residues in foodstuffs. The World Health Organization (WHO) defines the acceptable daily intakes (ADIs) of these residues as the dose of these antimicrobials that is safe for an average human to consume on a daily basis. We hypothesized that the lowest dose of ciprofloxacin classified as safe by the WHO could select for ciprofloxacin-resistant strains of Klebsiella pneumoniae in a Galleria mellonella model. Objectives: We aimed to evaluate if the consumption of peri-ADI doses of ciprofloxacin could select for ciprofloxacin-resistant (Ser464Phe, GyrB, ciprofloxacin MIC of 4 µg/mL) compared to -susceptible (isogenic, ciprofloxacin MIC of 0.047 µg/mL) strains of K. pneumoniae in a Galleria mellonella model. Results: A significant increase was seen in the proportion of resistance for the 1× ADI and 1/10th ADI concentrations on day 2 compared to the positive control. Methods: A model of K. pneumoniae infection in G. mellonella larvae was used for the experiment. The larvae were inoculated with K. pneumoniae followed by 10× ADI, 1× ADI, 1/10th ADI, 1/100th ADI, and 1/1000th ADI doses of ciprofloxacin. The isolation of K. pneumoniae colonies was then performed on selective agar plates with and without ciprofloxacin (1 µg/mL). The proportion of colonies with ciprofloxacin resistance was then calculated for each group at 24 and 48 h. Conclusions: We found that, at 48 h, there was an enrichment of K. pneumoniae colonies with ciprofloxacin resistance in the larvae receiving 1× ADI and 1/10th ADI concentrations of ciprofloxacin. These results suggest that the ciprofloxacin MSCselect for K. pneumoniae in this model is 1/10th of the acceptable daily concentration (ADI) dose of ciprofloxacin, which is equivalent to 0.239 ng/µL.

Implementation of Guidelines for Food and Meals in Kindergartens: Perspectives from Norwegian Kindergarten Staff

AbstractEstablishing dietary food habits in the early years is crucial. Kindergarten is a significant public health arena that can foster healthy eating habits regardless of socioeconomic status. In Norway, 93.4% of children aged 1–5 years attend kindergarten, and since most of their daily food intake is in kindergarten, it is important to make healthy food available. National guidelines on food and meals in kindergartens encourage kindergartens to engage in food and meal practices that promote health, well-being, development, and learning. Despite the guidelines, food and meal practices in kindergartens are highly varied, and implementation of the guidelines is lacking. To understand the challenges in implementing the guidelines, we conducted semi-structured interviews with sixteen staff representing sixteen kindergartens throughout Norway. Both public and private kindergartens were included. All interviews were recorded and transcribed verbatim, and content analysis was performed. The analysis identified three primary themes reflecting staff perceptions of challenges in implementing the national guidelines: priority and structural work with food and meals, pedagogical approach to food and meals, and external expectations and social pressure regarding food and meals. The themes were seen in light of the Consolidated Framework for Implementation Research in the discussion. While external conditions, including municipal prioritization, external expectations, and social pressure, play significant roles, our findings suggest that the most substantial influences on implementation are internal factors, particularly leadership and individual factors.

How to improve sustainability of nutrient dense diets for children and adolescents: an exemplary assessment in Germany.

The global climate crisis requires a paradigm shift in dietary concepts, respecting the needs of children. A global reference diet has been suggested by the EAT-Lancet Commission. On this basis, the detailed "Planetary Health Diet Index" (PHDI) has been proposed. The objective of this assessment is (1) to apply the PHDI to the Food-Based Dietary Guidelines, the so-called Optimized Mixed Diet (OMD) for children and adolescents in Germany in its original composition and (2) to check how the planetary value of the OMD could be improved by modifying food selection within meals while keeping the high nutrient densities of the guideline diet. The PHDI specifies 16 food groups and their proportion of total daily energy intake. The PHDI of the original OMD was calculated by assigning the foods of the 7-day menu to the PHDI food groups in order to score them. In this way, it became apparent which food groups had the potential to improve the sustainability. The diet was then updated by either reducing or increasing individual foods from these food groups in the meals and deriving the resulting PHDI. The nutrient densities of the original and updated daily OMD were calculated. The original diet obtained a PHDI score of 68.24 points, representing 45.5% of the theoretical maximum of 150 points. The following food groups achieved 9.9 to 10 out of 10 points: fruits, total vegetables, fish&seafood, vegetable oils, chicken (and substitutes). Conversely, food groups receiving a zero score included tubers&potatoes, dairy, red meat, animal fat, and added sugars. The updated diet resulted in increased consumption of 'nuts&peanuts', 'legumes', 'green vegetables', 'whole grains', and decreased consumption of 'tubers&potatoes' and 'red meat'. Overall, the PHDI increased from 68.24 to 81.51 points with the updated OMD, reflecting a 13.27% increase compared to the original diet. The nutrient densities were not significantly affected, but even slightly increased for most nutrients. The PHDI was applied to demonstrate how the sustainability of the guideline diet for children and adolescents in Germany could be improved through changes in individual food groups that can be easily implemented in practice while maintaining high nutrient densities and acceptability for children. NA.

Influence of milling processing on mineral and proximate composition of maize products

AbstractMaize and maize products are major staples consumed worldwide and contain dietary nutrients essential for human nutrition. Processing methods influence the safety and nutritional quality of maize products. This study investigated the effect of different milling processing factors on the mineral and proximate composition of maize products. The study also estimated the contribution of maize products to daily mineral intake. An atomic absorption spectrophotometer was used to determine the mineral content of samples. Proximate composition was determined using methods of the Association of Official Analytical Chemists. A multiple 24‐h dietary recall was used to estimate daily mineral intake of respondents who consume maize products in a cross‐sectional study. The results obtained showed that milling methods influenced the mineral and proximate compositions of the maize products. Mineral content in the milled maize samples followed the order P > Mg > Zn > Fe. The wet milling method retained the highest Fe and P contents, whereas the dried milled sample showed an increased Mg content. The estimated daily mineral intakes of participants were below the World Health Organization recommended standard limits. The respondents were at risk of deficiency of the minerals Fe and Mg. Public health education on sufficient dietary intake of these essential minerals should be intensified.

Correlation between intestinal microbiota and urolithin metabolism in a human walnut dietary intervention.

This study is to investigate the relationship between the intestinal microbiota and urine levels of the ellagic acid-derived polyphenols, the urolithins, in a cohort of subjects following a three-week walnut dietary intervention. We longitudinally collected fecal and urine samples from 39 subjects before and after walnut consumption (2oz per day for 21days). 16S RNA gene sequencing was performed on fecal DNA to study the association between microbiota composition and the levels of nine urolithin metabolites, which were measured using UHPLC/Q-TOF-MS/MS. Fecal microbial composition was found to be significantly different between pre- and post-walnut intervention (beta diversity, FDR-p = 0.018; alpha diversity, p = 0.018). Roseburia, Rothia, Parasutterella, Lachnospiraceae UCG-004, Butyricicoccus, Bilophila, Eubacterium eligens, Lachnospiraceae UCG-001, Gordonibacter, Paraprevotella, Lachnospira, Ruminococcus torques, and Sutterella were identified as the 13 most significantly enriched genera after daily intake of walnuts. We observed 26 genera that were significantly associated with 7 urolithin metabolites, with 22 genera positively correlating after walnut supplementation (FDR-p ≤ 0.05). PICRUSt analysis showed that several inferred KEGG orthologs were associated with 4 urolithin metabolites after walnut intake. In this study, we found that walnut supplementation altered urolithin metabolites, which associates with specific changes in bacterial taxa and inferred functional contents.

Occurrence of per- and polyfluoroalkyl substances in wheat, maize, rice, and soybean from chinese major grain producing regions.

Dietary exposure to per- and polyfluoroalkyl substances (PFAS) has been a concern for many years. Previous studies demonstrated that foods such as meat, milk, fish, vegetables, and tuber crops could be potential sources of human exposure to PFAS. However, research on PFAS contamination in grains remains limited. Therefore, this study conducted a comprehensive investigation of PFAS contamination in raw grains from major grain-producing regions in China. Among all grain species, soybeans exhibited the highest Σ16PFAS level (1.01ng/g dw), followed by rice (0.570ng/g dw), wheat (0.542ng/g dw), and maize (0.245ng/g dw). Short-chain perfluoroalkyl carboxylates (PFCAs) displayed higher detection frequencies compared to their long-chain homologues and perfluoroalkyl sulfonates (PFSAs). Perfluorobutanoic acid (PFBA) and perfluorooctanoic acid (PFOA) were dominant in wheat, maize, and soybean samples, while sodium p-perfluorous nonenoxybenzenesulfonate (OBS) was predominant in rice. OBS contributed approximately 70% to Σ16PFAS concentrations in rice. The concentration difference of OBS was not significant between indica rice and japonica rice, however, a disparity was observed between early indica rice and mid-late indica rice, suggesting that the growing period potentially affects OBS level in rice grains. Compared to other regions, east China displayed relatively higher PFAS concentrations in grain samples. This finding is consistent with previous studies and may be attributed to the intensive industrial and human activities. The estimated daily intake (EDI) of Σ16PFAS in grains from this study ranged from 0.0829 to 3.32ng/kg bw/day. The dietary health risks associated with PFOA in wheat and OBS in rice warrant further attention.

Four-Week GLP Immunotoxicity Assessment of Lactoferrin Alpha Produced by Komagataella phaffii in Sprague-Dawley Rats.

Oral toxicity and toxicokinetic properties of human lactoferrin (LF) alpha produced in Komagataella phaffii (effera™) were investigated in adult Sprague-Dawley rats over a 28-day period under good laboratory practice conditions. Main study dosing used groups of 10 rats/sex/dose, and a secondary study evaluating toxicokinetic parameters used 6 rats/sex/dose. The vehicle control group received sodium citrate buffer, test groups received daily doses of 200, 600, and 2000mg of effera™ per kg body weight, and the comparative control group received 2000mg bovine LF (bLF)/kg body weight per day. T-cell-dependent antibody response against keyhole limpet hemocyanin and immunophenotyping of the spleen were performed as measures of immunotoxicity. Clinical observations, body weight, hematology, coagulation, clinical chemistry, urinalysis, immunotoxicity, gross necropsy, and histopathology were assessed. Toxicokinetic parameters were analyzed as an indication of LF bioavailability, and anti-LF antibody assays were conducted to detect antibodies produced against LF to measure immunogenicity. No treatment related toxicologically significant changes were observed. Based on the absence of toxicologically relevant changes, effera™ is well tolerated in rats at doses up to 2000mg rhLF/kg/day, an amount ∼400 times that of the estimated daily intake at the 90th percentile proposed for human adult use.

Characteristics, prediction, and risk assessment of phthalates, organophosphate esters, and polycyclic aromatic hydrocarbons in vegetables from plastic greenhouses of Northeast China.

We investigated the contaminations of phthalates (PAEs), organophosphate esters (OPEs), and polycyclic aromatic hydrocarbons (PAHs) in the vegetables and their corresponding soils from 26 plastic greenhouses of Northeast China. PAEs, OPEs, and PAHs in the edible portion of vegetables were in the range of 2620-21800, 115-852, and 32.4-602 ng/g, while the levels of these chemicals in the greenhouse soils were 5770-18800, 196-935, and 109-1600 ng/g, respectively. PAEs are the main organic pollutants in greenhouses, which were 1-2 orders of magnitude higher than that of OPEs and PAHs. Leafy vegetables showed the highest contamination level, which is ∼1-3 times that of root and fruit vegetables. Bioaccumulation factors (BAFs) of chemicals are significantly negatively correlated with their physicochemical properties, e.g., octanol-water partition coefficient and organic carbon partition coefficient. The partition-limited model can accurately predict the contamination level of greenhouse vegetables to a certain extent based on the chemical's concentration in the corresponding soil. We assessed the hazard quotients of target compounds through daily intake of greenhouse vegetables, and found a low risk for di(2-ethylhexyl) phthalate. This research emphasized the potential dietary exposure risks caused by greenhouse leafy vegetables, and proposed a method for evaluating the risk of greenhouse vegetables through soil monitoring.

Unhealthy Alcohol Use: Screening and Behavioral Counseling Interventions.

Despite the increase in daily alcohol intake in recent decades and the implementation of national health screenings, effective management strategies for alcohol consumption remain outdated. This review evaluates intervention studies on screening and behavioral counseling for unhealthy alcohol use, with the aim of enhancing the effectiveness of interventions and improving health outcomes. On the basis of the GRADE (Grading of Recommendations Assessment, Development, and Evaluation)- ADOLOPMENT framework, systematic reviews and randomized controlled trials were examined to investigate the effectiveness of screening and counseling interventions in reducing unhealthy alcohol use. Five key questions were generated, and an evaluation and quality assessment of existing systematic reviews and new evidence related to each key question were conducted. Updating the U.S. Preventive Services Task Force and Cochrane 2018 reviews, we identified five new randomized trials that evaluated screening and counseling interventions for unhealthy alcohol use. For Key Question 2, the sensitivity and specificity of the new screening studies were consistent with those of prior research. Brief interventions were confirmed to reduce alcohol use (Key Question 4a), although additional research is required for a wider array of health outcomes. One study highlighted the benefits of counseling interventions for newborn health indicators in pregnant women (Key Question 4b). No new evidence was found regarding the harms of screening (Key Question 3) or alcohol use reduction interventions (Key Question 5). This review supports the continued use of brief interventions to reduce alcohol consumption in highrisk groups and highlights the need for culturally tailored research in Korea.

Biochar co-compost increases the productivity of Brassica napus by improving antioxidant activities and soil health and reducing lead uptake

Lead (Pb) is a serious toxic metal without any beneficial role in the biological system. Biochar (BC) has emerged as an excellent soil amendment to mitigate Pb toxicity. The impact of BC co-compost (BCC) in mitigating the toxic impacts of Pb has not been studied yet. Therefore, this study aimed to evaluate the potential of BC and BCC in improving the growth, physiological, and biochemical traits of Brassica napus and soil properties and reducing health risks (HR). The study was comprised of different Pb concentrations (control and 100 mg kg-1) and organic amendments (control, BC, compost, and BCC). The results indicated that Pb stress reduced the growth, photosynthetic pigments, seed yield, and oil contents by increasing hydrogen peroxide (H2O2) production and Pb uptake and accumulation in plant tissues and decreasing photosynthetic pigment and nutrient availability. The application of BCC alleviated the adverse impacts of Pb and improved seed production (40.24%) and oil yield (11.06%) by increasing chlorophyll a (43.18%) and chlorophyll b (25.58%) synthesis, relative water content (23.89%), total soluble protein (TSP: 23.14%), free amino acids (FAA: 26.47%), proline (30.98%), APX (40.90%), CAT (32.79%), POD (24.93%), and SOD (33.30%) activity. Biochar co-compost-mediated increase in seed and oil yield was also linked with a reduced accumulation of Pb in plant parts and soil Pb availability and improved the soil-available phosphorus, potassium, total nitrogen, soil organic carbon (SOC), and microbial biomass carbon (MBC). Furthermore, BCC also reduced the bioaccumulation concentration, daily metal intake, hazard index, and target hazard quotient. In conclusion, application of BCC can increase the growth, yield, and oil contents of Brassica napus by improving the physiological and biochemical traits and soil properties and reducing the Pb uptake.

Comparative Effect of Feeding Maiwa and Pearl Millet as Dietary Energy Source for Broiler Chicken

This study compared the effects of feeding broiler chickens, maiwa and pearl millet as dietary energy sources. The effectiveness, performance, and carcass features of broiler chickens given varying amounts of maiwa and pearl millet as dietary energy sources were evaluated in a feeding trial/experiment. The experiment used 200 day-old broiler chickens. The experiment was divided into two phases: starter and finisher. Five experimental meals containing 20 and 23% crude proteins were created for each phase. For T1, T2, T3, T4, and T5, five experimental diets were developed with different inclusion levels of 0, 25, 50, 75, and 100% respectively. In a randomized completely block design (RCBD), each treatment was duplicated four times, with ten birds each replication. Feed and water were provided ad libitum from 2nd to the 9th week of age. The result of the experiment on percentage composition of the experimental diets indicated that crude protein and metabolizable energy are within the range adequate for raising broiler chickens. The daily feed intake values (53.80-57.05g; 120.30-124.35g; 82.79-85.35g) and the daily weight gain values (29.95-33.82g; 41.77-44.66g; 34.22-35.88g) obtained from the performance of broiler chickens revealed non-significant differences among the treatments in the three (3) phases. The dietary treatments had no effect on the carcass and visceral organ parameters of broiler chickens, with the exception of caecal weight and small intestine length, which differed significantly (P≤0.01), with values ranging from 0.41%-0.72% and 160.85-191.33cm, respectively. Thus, the study comes to the conclusion that broiler chickens can safely be fed maiwa and pearl millet along with other items as nutritional energy sources.

Abstract 4129709: Aspirin-Nanoparticle for Dual Therapies: Targeted Anti-Inflammatory and Prolonged Anti-Platelet Effects for Atherosclerosis

Background: The current unmet needs for aspirin usage in atherosclerosis lie in its short half-life and narrow indication for anti-platelet effects. Daily aspirin intake is mandatory, and the anti-inflammatory effects of aspirin for atherosclerosis have not successfully translated to clinical practice. Nanoparticles remain in circulation for 2-3 days, with a large portion being cleared by splenic monocytes, which are known to inherently target inflamed sites. Hypothesis: By altering the pharmacokinetics of aspirin through loading into nanoparticles, aspirin-nanoparticles can exert prolonged anti-platelet effects and target atherosclerosis sites via monocyte carriers for anti-inflammatory effects, resulting in dual therapies. Methods: Splenic monocytes were loaded with aspirin-liposomes and co-cultured with endothelial cells or platelets to examine the interactions between them using high-resolution time-series microscopy. Prolonged anti-platelet effects and targeted anti-inflammatory effects of aspirin were validated in intact mice and hindlimb ischemia models, respectively. Furthermore, the dual therapies of aspirin-liposomes were validated in an atherosclerotic mouse model created by partial carotid ligation and a western diet in apoE gene knock-out mice. Results: When splenic monocytes were loaded with aspirin-liposomes, they emitted extracellular vesicles (EVs) loaded with aspirin towards endothelial cells or platelets. As inflamed cells upregulate caveolin expression, they uptake an increased amount of transferred EVs compared to non-inflamed cells. Additionally, aspirin-liposomes showed prolonged circulation time and increased splenic accumulation compared to aspirin itself, resulting in prolonged anti-platelet effects (>7 days) and targeted anti-inflammatory effects at inflamed sites. Compared to the daily oral aspirin group in the atherosclerosis model, the weekly intravenous aspirin-liposome group showed superior therapeutic effects, including attenuated systemic and local inflammation and patent lumen in atherosclerotic sites. Conclusion: Aspirin-nanoparticles can exert prolonged anti-platelet effects combined with targeted anti-inflammatory effects, resulting in superior therapeutic effects on atherosclerosis.

Food security and nutritional vulnerability in Comoros: The impact of Russia-Ukraine conflict.

The Sustainable Development Goal 2 aims to eradicate hunger. However, many small island developing states (SIDS) face challenges in achieving this goal due to their reliance on the global market and susceptibility to crises. This paper focuses on Comoros, one of the least studied SIDS located in the Indian Ocean, to examine its food security and nutritional vulnerability. The findings reveal that Comoros experiences limited access to sufficient and nutritious diets, which are excessive in fat and deficient in key micronutrients. This situation is similar to other SIDS in the Pacific and the Caribbean, which also exhibit the convergence of the malnutrition triple burden. This paper further assesses the impact of changing food prices during the initial year of the Russia-Ukraine conflict on food security and nutrition in Comoros, serving as a case study for SIDS. Using microsimulations, we estimate the effects on food access, sufficiency, and adequacy at the household level. The results indicate a decline in food access and purchasing power for all households, particularly the poorer and rural. Additionally, food sufficiency has markedly decreased, leading to an additional 5,000 households (3.3% of all households) experiencing inadequate daily calorie intake. The study also highlights a reduction in the consumption of organ meats, fruits, and vegetables, resulting in decreased daily iron and folate intakes. This is particularly concerning given the high prevalence of anemia among women in Comoros and other SIDS. To address these challenges, policies promoting the consumption of fresh, nutrient-rich foods with low fat content are crucial to mitigate the malnutrition triple burden and curb the increasing trend of non-communicable diseases in these countries.

Impact of fruits and vegetables consumption on metabolic health: a case–control study

Abstract Background According to the World Health Organization (WHO), it is recommended to consume more than 400 grams of (fruits + vegetables) per day to enhance healthful lifestyle and to lower the incidence of metabolic illnesses. Their low glycemic index helps prevent spikes in blood sugar, consequently controlling hunger. Our aim was to study the reflection of the intake of fruits and that of vegetables on metabolic risks in obese children. Results A case–control research enrolled 90 Egyptian children (44 obese and 46 normal weight control children) aged from 5 to 11 years. Thirty-three out of 46 controls (71.7%) consumed vegetables on a daily basis compared to only 27 (61.4%) among cases. As regards fruits, the daily consumption was frequented more among the obese group 25 (58.1%) and less frequented among the control group 20 (43.5%). No significant associations were found between vegetables/fruits’ consumption and the specified metabolic markers of dyslipidemia and hyperglycemia. Conclusion The role of fruits and vegetables is still debatable. In our study, the daily intake of fruits and vegetables was not significantly associated with changes in blood sugar or lipid levels.

Hazardous alcohol consumption is associated with an increased occurrence of falls among people with HIV in the PROSPER-HIV Study.

Falls are a significant public health concern, particularly among older adults and people with HIV (PWH). This study examines the association between alcohol consumption and falls in PWH. The PROSPER-HIV study recruited PWH from four US sites. Participants were categorized based on Alcohol Use Disorders Identification Test Consumption (AUDIT-C) scores: none, non-hazardous, and hazardous drinking. Data collection included demographics, medical history (i.e., comorbidities, treated hypertension, eGFR), alcohol consumption using AUDIT-C, daily alcohol recall in grams, and self-reported falls over the previous year. Physical performance was measured using the Short Performance Physical Battery (SPPB). Statistical analyses included Pearson's correlation and Poisson regression models to estimate fall prevalence ratios (PR), adjusting for confounders (SPPB, comorbidities, treated hypertension, and eGFR). The study included 315 PWH, aged 52 ± 12 years, with 78% male participants. Thirty-three percent were classified as non-drinking, 50% non-hazardous, and 17% hazardous drinking. Poisson regression showed a significantly higher risk of falls (PR: 2.12, 95% CI: 1.11-4.03) and recurrent falls (PR: 3.54, 95% CI: 1.21-10.3) among hazardous drinking compared to non-hazardous drinking, even after adjusting for confounders. The PR for falls per daily intake in grams was not statistically significant. There is a significant association between hazardous alcohol consumption and increased fall risk in PWH using AUDIT-C, but not when accessing recall of alcohol consumption in grams.

Feeding chicory silage, but not Se-yeast or a single injection of inorganic Se, affects metabolism, fat in milk, and type I immunity in transition ewes

In the study, we assessed the effect on performance and health of a single injection of inorganic Se prepartum or feeding chicory silage and organic Se supplementation during the peripartum in ewes. Approximately one month before lambing, 45 pregnant Polypay ewes were moved into single pens and randomly assigned to 5 groups to be fed either grass or chicory silage and supplemented or not with 3.6 mg Se/day as selenium yeast or given a single prepartum injection of Na-selenite. Daily dry matter intake (DMI), water intake, milk production and components, blood metabolic, immune and inflammatory parameters, and blood micromineral levels were measured. DMI was lower in ewes fed chicory silage, although no statistical differences in milk yield were observed. Very few differences were observed in milk components, except fat %, which was higher among ewes fed chicory silage. The type of silage had a significant effect on the fatty acid profile of the milk, with the milk from ewes fed chicory having a higher proportion of unsaturated fatty acids and overall improved health indices compared to the milk from ewes fed grass silage. Blood NEFA and BHBA were higher in ewes fed chicory vs. grass silage. Neither silage type nor Se supplementation had a strong effect on most of the parameters associated with immune or inflammatory function, except for the liver enzymes GGT and GOT, which were lower, and a larger type I/type II ratio immune response measured by the DxD2 assay among ewes fed chicory vs. grass silage. No effects on parasite fecal egg counts were observed. Supplementation of ewes with Se-yeast resulted in higher blood levels of Se, whereas the one-time prepartum injection had no significant effect on whole blood Se levels. Feeding chicory silage and supplementing Se during the transition period had a minimal impact on ewe performance and health.

Antibiotic Residues in Milk and Milk-Based Products Served in Kuwait Hospitals: Multi-Hazard Risk Assessment

Antimicrobial resistance (AMR) poses a significant global health challenge affecting food safety and development. Residues of antibiotics in food from animal sources, particularly milk, contribute to the development and spread of AMR, alter intestinal microbiota, and potentially lead to allergies, serious health conditions, and environmental and technological problems within the dairy industry. Therefore, this study investigated the residue levels of veterinary drugs from β-lactam antibiotics and tetracyclines in milk and milk products and assessed human health risks. Two hundred milk and milk product samples (pasteurized milk, sterile milk, soft white cheese, and processed cheese, 50 each) were collected from different hospitals in the State of Kuwait and screened for antibiotic residues using a microbial inhibition assay (Delvotest SP-NT) and high-performance liquid chromatography (HPLC). Delvotest SP-NT and HPLC analyses showed that 30, 28, 26, and 24% of the pasteurized milk, sterilized milk, white soft cheese, and processed cheese samples tested positive for antibiotic residues. Forty-eight milk and cheese samples were confirmed as positive by both methods, and six samples initially found to be negative by Delvotest SP-NT were confirmed as positive by HPLC. Multi-antibiotic residues were detected in five samples by using HPLC. The kappa coefficient (0.921; p < 0.0001) revealed complete concordance between the HPLC and Delvotest SP-NT results. Ampicillin was the most abundant residue in the positive samples (31.48%), ranging from 2.44 to 3.89 μg/L, with an overall mean concentration of 3.492 ± 0.094 μg/L, followed by tetracycline and oxytetracycline (27.78% each), ranging from 54.13 to 220.3 μg/L and from 41.55 to 160.7 μg/L, with mean concentrations of 129.477 ± 14.22 and 91.86 ± 9.92 μg/L, respectively. The amoxicillin levels in the samples (12/54; 22.22%) ranged from 3.11 to 5.5 μg/L, with an overall mean concentration of 3.685 ± 0.186 μg/L. The maximum concentrations of ampicillin, amoxicillin, and tetracycline were detected in processed cheese with mean concentrations of 3.89 ± 0.28 µg/L, 3.95 ± 0.15 µg/L, and 170.3 ± 0.27 µg/L, respectively. Pasteurized milk contained the maximum concentrations of oxytetracycline, with a mean concentration of 120.45 ± 0.25 µg/L. The tetracycline residues exceeded the standard maximum residue limits (MRLs; 100 µg/L) in 6% of both pasteurized and sterilized milk samples, and in 4% of processed cheese. Additionally, the oxytetracycline levels in pasteurized milk (6%) and amoxicillin levels in processed cheese (2%) were higher than the permitted MRLs (100 µg/L and 4 µg/L, respectively). Furthermore, the antibiotic residues detected in 12.5% (25/200) of the samples were close to standard permissible MRL limits for ampicillin (5%), amoxicillin and oxytetracycline (3% each), and tetracycline (1.5%). Hazard quotients, which compare the standard acceptable daily intake (ADI) to the estimated daily exposure (EDI), indicated that the overall risk associated with antibiotic residues in these dairy products is low. The EDI was lower than the ADI for the tested antibiotics, indicating an elevated safety margin. While the overall hazard quotients are low, the potential for the development of antibiotic resistance due to long-term exposure to low levels of antibiotics should be considered. Hence, strict regulations and enforcement are necessary to prevent excessive residue levels and to promote responsible antibiotic use in dairy production. Regular monitoring of antibiotic residues in dairy products is essential for ensuring consumer safety.

Estimated human intake of endogenous and exogenous hormones from beef in the United States.

Endogenous and exogenous hormones may be present in beef. Human consumption of hormones has been linked to adverse health effects. To estimate daily intake of hormonal growth promotants (HGP) from beef consumed by the US population. We combined self-reported beef consumption information from a nationally-representative survey with concentrations of 12 HGP measured in 397 samples of retail beef/fat purchased in California. We defined typical, high, and maximum intake scenarios assuming self-reported consumed beef contained the mean, 95th percentile, and maximum concentrations of each HGP, respectively. We estimated distributions of usual (i.e., long-term) daily intake and short-term daily intake (µg/kg/day). We calculated the hazard quotient (HQ), or ratio of estimated intake to the World Health Organization's acceptable daily intake (ADI) for the HGP. The highest estimated HQs were found for melengestrol acetate (MGA). For usual daily intake under the typical intake scenario, no HQ exceeded 0.02 (0.00047 µg MGA/kg/day). Under the maximum intake scenario, the highest HQ was 0.29 (0.0087 µg MGA/kg/day), corresponding to the 99th percentile of intake among young boys (ages 1-5). The highest short-term intake estimates for MGA under the maximum intake scenario were the 99th percentile of intake among young girls and boys, which equaled (HQ = 1.00) or exceeded (HQ = 1.29) the ADI for MGA, respectively. Hormonal growth promotants (HGP) are used to increase beef production and have been linked to adverse reproductive effects. We estimated daily intake of MGA and several other HGP using US nationally-representative beef consumption data collected between 2015-2018 and HGP concentrations in retail beef. Estimated intake was highest for young children, but estimates were generally very low compared to current health-based intake limits. However, these limits are typically based on studies in adult animals, and further study of potential adverse effects during sensitive developmental periods, such as in early life, may be warranted to ensure recommended intake limits are health-protective.

Calcium plus vitamin D supplementation during pregnancy has no impact on postpartum transient longitudinal changes in hip geometry in adolescent mothers: a secondary analysis of a randomised controlled trial.

We have previously demonstrated that calcium plus vitamin D supplementation during adolescent pregnancy reduces the magnitude of transient postpartum bone mass loss. In the present post hoc analysis, we further investigated the effect of calcium plus vitamin D supplementation during pregnancy in hip geometry throughout one year postpartum in Brazilian adolescents with low daily calcium intake (∼600 mg/d). Pregnant adolescents (14-19 years) were randomly assigned to receive calcium (600 mg/d) plus vitamin D3 (200 μg/d) or a placebo from 26 weeks of gestation until parturition. Dual-energy X-ray absorptiometry images were obtained at 5 (n 30 and 26 for calcium plus vitamin D and placebo, respectively), 20 (n 26 and 21) and 56 (n 18 and 12) weeks postpartum, and hip geometry parameters were analysed by Advanced Hip Assessment software. The effects of the intervention, time point and their interaction were assessed using repeated-measures mixed-effects models. No significant intervention effects or intervention × time interactions were observed on hip geometry parameters (P > 0·05). Time effects were observed in cross-sectional area, cross-sectional moment of inertia and section modulus parameters with decreases from the 5th to the 20th week postpartum followed by recovery from the 20th to the 56th week (P < 0·05). Our findings indicate that the postpartum period is associated with transient changes in the hip geometry of lactating adolescent mothers, regardless of the low calcium intake and the supplementation offered during pregnancy, suggesting that a physiological adaptation of these adolescents to low calcium intake is at play.