Cysteine Solution Research Articles

Study on removal of NO from flue gas by ferrous cysteine solution and analysis of reaction process

Wet denitrification technology has been extensively researched for its benefits in synergistically treating pollutants. As a representative complexing agent, ferrous cysteine (Fe2+(CyS)2) solution in the complex absorption method has attracted the attention of researchers. In this study, the influence of c(Fe2+):c(CySH) ratio, complexation time, initial pH value, and temperature on the NO removal efficiency and reaction process of Fe2+(CyS)2 in a bubbling reactor were investigated. The results highlighted the significance of the initial pH of the Fe2+(CyS)2 solution in denitrification efficiency, with higher pH leading to improved NO removal. The denitrification efficiency showed a volcano-like variation with increasing temperature and was not much affected by NO inlet concentration and Fe2+(CyS)2 solution complexation time. Under optimal conditions, the denitrification efficiency remained above 80 % for 3 h of continuous reaction, peaking at 98.4 %. Calculations of NO absorption capacity indicated the regeneration of Fe2+(CyS)2 solutions. Testing with iodometric and bromine-ferrous titration methods revealed that the reaction product of cysteine is cystine, maintaining material balance before and after the reaction. Experimental results validate Fe2+(CyS)2 as an effective and stable denitrification method, laying the foundation for potential industrial application.

PRODUCTOS ALTERNATIVOS EN LA DESINFESTACIÓN IN VITRO EN EXPLANTES DE PORTAINJERTO DUKE-7 E IDENTIFICACIÓN DE MICROORGANISMOS ENDÓFITOS

<p><strong>Background. </strong>The vegetative material for <em>in vitro</em> establishment of avocado explants is from the field, for this reason, the proportion of contamination, oxidation and mortality of explants is elevated, therefore we are looking for alternatives to decreased. <strong>Objective. </strong>To evaluate concentrations of alternative products for disinfestation in avocado explants cv Duke 7, and decrease oxidation with the addition of L-cysteine in the <em>in vitro</em> establishment. <strong>Methodology. </strong>The products used were: peroxyacetic acid (AP), chlorine dioxide (DC), and quaternary ammonium salts (ACS), in three concentrations. 54 explants were used in each treatment, and placed in culture medium for establishment, evaluating contamination (%) and oxidation (%). Two weeks later, 50% of viable explants from each treatment were immersed in L-cysteine (100 mgL<sup>-1</sup>) and placed in a culture medium for multiplication, the rest was placed a in multiplication medium containing L-cysteine; only oxidation was evaluated (%). Contaminated explants were isolated, identified, and characterized fungi and bacteria. <strong>Results. </strong>The best results of the disinfestation were with ACS with 3.7% contamination, without oxidation. Regarding the use of L-cysteine (100 mgL<sup>-1</sup>), the lowest percentage of oxidation (47%) was obtained when immersed in a cysteine solution. Three genera of fungi were identified: <em>Aspergillus</em> (3), <em>Penicillium</em> (1) and <em>Cladosporium</em> (1), and <em>Agrobacterium</em> as a bacterium. <strong>Implications. </strong>With the proposed methodology, we can continue with the following stages of <em>in vitro</em> culture, to establish methodologies for budding, growth, and rooting of avocado clonal rootstocks. <strong>Conclusions</strong>. The use of ACS and cysteine in solution reduces both contamination and oxidation of explants, but it is necessary to modify concentrations and exposure times in the disinfestation protocol, in addition to identifying even the genus the microorganisms found.</p>

Effect of direct plasma treatment and plasma‐treated liquid on cutaneous squamous cell carcinoma: A proof‐of‐concept study

AbstractThis study investigates the efficacy of cold atmospheric plasma (CAP) in treating squamous cell carcinoma (SCC), a highly metastatic cancer. To assess the anticancer activity, two CAP treatment methods—direct treatment and indirect treatment (plasma‐treated N‐acetyl cysteine solution)—were used. Results indicate that both methods selectively target SCC cells, inducing oxidative and nitrosative stress, leading to decreased metabolic activity and increased apoptosis. Furthermore, a critical feature of cancer cells, metastatic behavior is also inhibited by CAP treatment. Coculture experiments demonstrate the effectiveness of CAP treatment in selectively inactivating SCC cells while leaving healthy cells unaffected. These findings suggest the potential use of CAP as a selective and effective treatment for SCC.

Application of antioxidants and hot water treatments to improve shelf life of fresh-cut eggplants (Solanum melongena L.) during storage

The objective of this study was to evaluate the effect of antioxidant treatments on the conservation of cut eggplants (Solanum melongena L.), which was carried out in two stages. Initially, the effect of citric acid (CA), ascorbic acid (AA) and cysteine (Cys) solution at 0.5 and 1% on sensory attributes (general appearance and browning), and color parameters during storage were evaluated. Immersion in 1% AA was considered the best antioxidant since it maintained visual quality for 6 days. Subsequently, hot water dipping (HWD) treatments followed by the 1% AA solution were evaluated and optimized through the Response Surface Methodology (RSM). Sensory attributes, color parameters, respiration rate (RR), phenolic compounds (PhC), antioxidant capacity, as well as the activity of polyphenol oxidase (PPO) and peroxidase (POD) were assessed during cold storage. The results showed that HWD at 50 °C, 60 s and 1% AA was the optimal combination to control enzymatic browning and extend its fresh quality for up to 8 days. Furthermore, that combination of treatments reduced the PPO and POD activities and increased the PhC compared to the control (untreated), not finding significant differences between them in antioxidant capacity and RR. Therefore, the application of this combination would be the most appropriate to preserve the quality of the fresh-cut eggplants for 8 days of storage at 4 °C.

Cysteine as an Alternative Eco-Friendly Corrosion Inhibitor for Absorption-Based Carbon Capture Plants.

Inorganic corrosion inhibitors are commonly applied to mitigate severe corrosion in absorption-based carbon capture plants. They are, however, not environmentally friendly, posing a health risk, harming the environment, and making chemical handling and disposal costly. Therefore, this study evaluated the corrosion inhibition performance of an amino acid, namely cysteine, with the aim of providing an eco-friendly alternative to the commercial inorganic corrosion inhibitors. Electrochemical and weight loss corrosion measurements showed that cysteine was effective in protecting carbon steel at all process operating conditions. At 80 °C, a 500 ppm cysteine could provide up to 83% and 99% inhibition efficiency under static and dynamic flow conditions, respectively. Its inhibition efficiency could be improved when the cysteine concentration, solution temperature, and flow condition were altered. Cysteine was an anodic corrosion inhibitor and underwent spontaneous, endothermic, and combined physical and chemical adsorption that followed the Langmuir adsorption isotherm model. The quantum chemical analysis indicated that cysteine had a high reactivity with metal surfaces due to its low energy gap and high dipole moment. The EDX analysis revealed a significant sulphur content on the metal substrate, indicating that cysteine's mercapto group played an integral role in forming an effective adsorption layer on the metal interface.

Enhanced corrosion resistance and biofunctionality of Zn–Al layered double hydroxide coating on micro-arc oxidized ZK60 Mg alloy via ion exchange

In this work, zinc-aluminum layered double hydroxides (Zn–Al LDHs) were prepared on the surface of micro-arc oxidized ZK60 magnesium (Mg) alloy by a hydrotreatment in a Zn(NO3)2 solution. Scanning electron microscopy, X-ray diffraction (XRD), electrochemical impedance spectroscopy (EIS), laser scanning confocal microscopy and Fourier-transform infrared spectroscopy (FT-IR) were applied to analyze the coating characteristics. Ion exchange experiments were performed on the optimized LDHscoatings with 0.1 M methionine (Met) and cysteine (Cys) solution for 4 h at 60 °C. Methionine and cysteine were successfully inserted into the interlayer structure of LDHs. The LDHs-Met and LDHs-Cys coatings are distributed in a petal shape by hexagonal nanosheets, and their distributions on the surface of the MAO are dense and uniform. The porosity, surface roughness, hydrophilicity, and corrosion resistance of LDHs-Met and LDHs-Cys coatings are increased compared to MAO and MAO/LDHs coatings. The amino acids in the LDHs could play an influential corrosion inhibition role, improving the corrosion resistance of the MAO effectively. The ion exchange layer has a more substantial protective effect on the substrate and better biocompatibility than MAO and MAO/LDHs coatings.

Influence of a transient spark plasma discharge on producing high molecular masses of chemical products from l-cysteine

Cold atmospheric pressure plasmas are considered a forthcoming method in many research areas. Plasma modification of biomolecules has received much attention in addition to plasma-treated biomaterials. Hence, in this work, we operated a transient spark plasma (TSP) discharge to study its effect on the l-cysteine chemical structure. the TSP was configured in a pin-to-ring electrode arrangement and flowed by Ar gas. We also investigated the effect of two chemicals; dimethyl sulfoxide (DMSO) and hydrogen peroxide (H2O2) by the bubbling method to show how they can change the creation of new chemical bioproducts. Ultraviolet–Visible absorption spectroscopy, Fourier transform infrared spectroscopy and Liquid chromatography–mass spectroscopy were used to investigate any changes in chemical bonds of cysteine structure and to depict the generation of new biomolecules. Based on the displayed results plasma-generated reactive species had a great role in the chemical structure of the cysteine. Entering DMSO and H2O2 into the plasma caused the creation of new products and the heaviest biomolecule was produced by the simultaneous addition of DMSO and H2O2. The results also predicted that some chemical products and amino acids with a higher value molecular masse produced from the polymerization process of cysteine solution. The strong oxidation process is responsible for the heavy chemical compounds.

Viability of seeds of two varieties of Coffea arabica L. using different pretreatments in the tetrazolium test

This research attempted to determine the efficacy of the tetrazolium test in the evaluation of the seed viability of two varieties of Coffea arabica L. (‘Castillo’ and ‘Cenicafé’). The fruits were obtained from crops located in the municipalities of Salazar de las Palmas and Arboledas (Norte de Santander - Colombia). The test was carried out with embryos manually extracted from the seeds using tweezers. Three pretreatments were established: distilled water, sodium hypochlorite (2.5 %), sucrose (10 %), and a control (no pretreatment). Embryos were placed in a cysteine solution (0.5 %) to prevent oxidation, then immersed in tetrazolium solutions with concentrations of 0.035 %, 0.075 %, and 0.1 % for a period of 6, 9, and 12 hours in darkness. The results of the viability test were validated with seed germination, using the wet paper towel method in darkness. The best viability percentages were found with the application of sodium hypochlorite (NaClO 2.5 %), with a high correlation with the germination percentage. The use of pretreatments improved the efficiency of the viability test and allowed the use of low concentrations of the reagent (0.035 %), giving the farmer a quick and less expensive alternative to determine germination capacity.

Simultaneous determination of nereistoxin insecticides in foods of animal origins by combining pH-dependent reversible partitioning with hydrophilic interaction chromatography-mass spectrometry

Although nereistoxin insecticides (NIs) are banned for animal husbandry operations, they are still used because of their high insecticidal activities. Therefore, a reliable residue analysis method for the simultaneous detection of cartap, bensultap, thiocyclam, and nereistoxin in foods of animal origins, including beef, pork, chicken, milk, and eggs, was developed using hydrophilic interaction liquid chromatography–mass spectrometry (HILIC–LC–MS/MS). The NIs were extracted with an acidic cysteine and formate buffer solution and hydrolyzed to nereistoxin. The molarity and pH of the buffer were optimized at 20 mM and 3, respectively, to keep the pH of the extracts at 4–5. pH-dependent acid–base partitioning coupled with salting-out-assisted liquid–liquid extraction using acetonitrile was performed for purification and for the direct introduction of the extracts to LC. The optimal pH values were 5 and 9 for the acid–base partitioning. Nereistoxin quantitation was achieved with consistent column retention (RSD < 0.6%) and a high degree of separation (N > 106). The matrix-dependent method limit of quantitation was 2 μg nereistoxin/kg, and the calibration curve showed good linearity (R2 > 0.998). The recovery efficiencies were in the range of 89.2–109.9% with relative standard deviations less than 10%, and matrix effects did not exceed ± 10%, which satisfied the criteria outlined in the European SANTE/12682/2019 guidelines.

Corrosion in Tinplate Cans Used for Food Storage, Part 3: Effects of Cysteine, NaCl, and Tomatoes on Corrosion of Tin, Iron, and Tinplate

Tinplate cans used to contain foodstuff are fabricated from steel coated with a thin layer of tin and then overcoated with a thin polymeric layer. Previous papers in this series investigated the corrosion behavior of tinplate cans containing chopped tomatoes or one of the chemical components found in cans of chopped tomatoes. The amino acid cysteine was found to be an aggressive agent that can cause corrosion and blackening of the can headspace region. In this study, the corrosion phenomena associated with tinplate cans were investigated by studying the behavior, individually, of uncoated pure tin, iron, and tinplate in chopped tomato, NaCl, and cysteine solutions using electrochemistry, optical microscopy, x-ray diffraction, scanning electron microscopy, and Raman spectroscopy. Black corrosion products, identified as sulfides, formed when the tin, iron, and tinplate specimens were exposed to cysteine solution at three different pH values. The black corrosion products were also found on both iron and tinplate when the samples were immersed in tomato solution. These results indicate that blackening on bare iron and tinplate could form easily from the decomposition of chemical compounds in heated tomatoes.

Bioconjugated technetium carbonyls by transmetalation reaction with zinc derivatives

The transmetalation reaction between zinc dithiocarbamates functionalized with organic groups and the cation fac-[99mTc(H2O)3(CO)3]+ has been studied as a new strategy to bind biomolecules to this radionuclide for preparing radiopharmaceuticals with high molar activity. All complexes were obtained in high yields by heating at moderate temperatures and without subsequent purification. The chemical identity was ascertained by HPLC comparison with the homologous rhenium complexes. Stability studies in cysteine solution and serum have shown a good stability of the coordination set fac-[99mTc(CO)3(SS)(P)]. Preliminary biological studies of the radiocomplex functionalized with D-(+)-glucosamine with carcinoma cells have been performed.

Fabrication of a sensitive colorimetric nanosensor for determination of cysteine in human serum and urine samples based on magnetic-sulfur, nitrogen graphene quantum dots as a selective platform and Au nanoparticles

A novel colorimetric nanosensor is reported for the selective and sensitive determination of cysteine using magnetic-sulfur, nitrogen graphene quantum dots (Fe3O4/S, N-GQDs), and gold nanoparticles (Au NPs). Thus, S, N-GQDs was firstly immobilized on Fe3O4 nanoparticles through its magnetization in the presence of Fe3+ in the alkali solution. The prepared Fe3O4/S, N-GQDs were dispersed in cysteine solution resulting in its quick adsorption on the surface of the Fe3O4/S, N-GQDs through hydrogen bonding interaction. Then, Au NPs solution was added to this mixture that after a short time, the color of Au NPs changed from red to blue, the intensity of surface plasmon resonance peak of Au NPs at 530 nm decreased, and a new peak at a higher wavelength of 680 nm appeared. The effective parameters on cysteine quantification were optimized via response surface methodology using the central composite design. Under optimum conditions, the absorbance ratio (A680/A530) has a linear proportionality with cysteine concentration in the range of 0.04–1.20 μmol L−1 with a limit of detection of 0.009 μmol L−1. The fabrication of the reported nanosensor is simple, fast, and is capable of efficient quantification of ultra traces of cysteine in human serum and urine real samples.

Ultrasound responsive carbon monoxide releasing micelle

Carbon monoxide (CO), an endogenously produced gasotransmitter, has shown various therapeutic effects in previous studies. In this work, we developed an ultrasound responsive micelle for localized CO delivery. The micelle is composed of a pluronic shell and a core of a CO releasing molecule, CORM-2. The mechanism is based on the ultrasound response of pluronics, and the reaction between CORM-2 and certain biomolecules, e.g. cysteine. The latter allows CO release without significantly breaking the micelles. In a 3.5 mM cysteine solution, the micelles released low level of CO, indicating effective encapsulation of CORM-2. Treatment with a low intensity, non-focused ultrasound led to four times as much CO as the sample without ultrasonication, which is close to that of unencapsulated CORM-2. Significantly reduced proliferation of prostate cancer cells (PC-3) was observed 24 h after the PC-3 cells were treated with the CORM-2 micelles followed by ultrasound activation.

Black phosphorus quantum dots are useful oxidase mimics for colorimetric determination of biothiols.

Black phosphorus quantum dots (BP QDs) with small size are synthesized usingan easy to operate thermal method. It was found that BP QDs possess oxidase-mimicking activity. They can catalyze the oxidation of thesubstrate 3,3',5,5'-tetramethylbenzidine to produce a blue-colored product even in the absence of hydrogen peroxide. Active oxygen species are proved to be involved in the reaction through the experiments of radical scavenging and electron spin resonance. Biothiols including reduced glutathione and cysteine inactivate the oxidase-mimicking activity of BP QDs, concomitant to the fading of the blue solution. Thisprovides the base fora colorimetric method for the determination of glutathione and cysteine. The decreased absorbance at 652nm displays linear response to the concentrations of glutathione ranging from 0.1 to 5.0μmolL-1, and cysteine from 0.1 to 10.0μmolL-1. The detection limits are 0.02μmolL-1 and 0.03μmolL-1 for glutathione and cysteine, respectively. Successive determinations of 1.0μmolL-1 glutathione and 5.0μmolL-1 cysteine solution give relative standard deviations of 0.8% and 1.7% (n = 11), respectively. As a preliminary application, the practicability of the method was evaluated by the determination of glutathione in pharmaceutical preparations. This work not only discovers a useful oxidase mimics but also sets up a reliable platform based on BP QDs in colorimetric detection. Graphical abstract Schematic representation of colorimetric determination for biothiols through inactivating oxidase mimetic-like catalytic activity of black phosphorus quantum dots(BP QDs) on the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB)with dissolved oxygento produce its blue oxidized product(oxTMB).

Plug-and-Play Continuous Gas Flow Assembly of Cysteine-Inserted AuCu Nanobimetals for Folate-Receptor-Targeted Chemo-Phototherapy.

Conjugatable nanobimetals exhibiting broadband light absorption for use as phototherapeutic platforms were assembled via a plug-and-play continuous gas flow route. Electrically produced AuCu nanobunches (NBs) under nitrogen gas flow were directly injected into cysteine (cys) solution through gas pressurization to mechanically spray the solution (AuCu into cys droplets). The sprayed droplets were then exposed to 185 nm UV light (higher photon energy [6.2 eV] than the work functions of Au [5.1 eV] and Cu [4.7 eV]) to initiate photoionization of AuCu NBs for subsequent electrostatic reaction with the SH- group of cys to form cys-inserted AuCu (AuCu-cys) platforms in a single-pass gas stream. These platforms exhibited broadband light absorption spectra because of hybridized interparticle plasmonic coupling and could be conjugated to folic acid (FA) when dispersed in FA solution to form highly dispersible, biocompatible, and cancer-targetable AuCu-cys-FA. This material was suitable for use in targeted phototherapy of folate-receptor (FR)-rich cancers via FR-mediated endocytosis, and loading doxorubicin (DOX) into AuCu-cys-FA (i.e., AuCu-cys-DOXFA) facilitated chemo-phototherapy because of photoresponsive anticancer drug release upon induction of hyperthermia.

System for mercury preconcentration in natural waters based on a polymer inclusion membrane incorporating an ionic liquid.

In this study, we have evaluated two different ionic liquids (IL) as extractants based on the same cation (trioctylmethylammonium) but bearing the anion thiosalicylate (TOMATS) or salicylate (TOMAS). Both IL have been incorporated as carriers in polymer inclusion membranes (PIMs), and mercury (Hg) has been preconcentrated using a special device. Results show that among the tested IL, TOMATS has given better results. A PIM made of 50% cellulose triacetate, 30% TOMATS and 20% nitrophenyl octyl ether as a plasticizer enabled the effective transport of Hg to a 10-3M cysteine solution used as a stripping phase. This novel and simple PIM-device system allows the transport of Hg at low concentration levels in different types of natural waters such as rivers, groundwater and seawater without any previous treatment. Since no matrix effect was observed on Hg transport efficiency with different waters, this newly developed PIM-system could be used as a global detection system for this metal. The effect of biofilm growth on the surface of PIMs has been investigated for the first time, and no significant differences on Hg transport have been found when using a fresh PIM and a PIM deployed for 7 days in a pond.

Native-MS Analysis of Monoclonal Antibody Conjugates by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.

Antibody-drug conjugates (ADCs) are an important class of therapeutic molecule currently being used to treat HER2-positive metastatic breast cancer, relapsed or refractory Hodgkin lymphoma, systemic anaplastic large cell lymphoma, relapsed or refractory B-cell precursor acute lymphoblastic leukemia, and acute myeloid leukemia. An ADC typically consists of a small molecule or peptide-based cytotoxic moiety covalently linked, via lysine or cysteine residues, to a monoclonal antibody (mAb) scaffold. Mass spectrometric (MS) characterization of these molecules affords highly accurate molecular weight (MW) and drug-to-antibody ratio (DAR) determination and is typically performed using orthogonal acceleration time-of-flight (oa-ToF) analyzers and more recently, Orbitrap instruments. Herein we describe for the first time the use of a 15 T solariX Fourier transform ion cyclotron mass spectrometer to characterize an IgG1 mAb molecule conjugated with biotin via native lysine and cysteine residues, under native-MS and solution conditions. The cysteine-biotin conjugates remained fully intact, demonstrating the ability of the FT-ICR to maintain the noncovalent interactions and efficiently transmit labile protein complexes. Native-MS was acquired and is displayed in magnitude mode using a symmetric Hann apodization function. Baseline separation is achieved on all covalent biotin additions, for each charge state, for both the lysine- and cysteine-biotin conjugates. Average DAR values obtained by native-MS for the lysine conjugate are compared to those derived by denaturing reversed phase liquid chromatography using an oa-ToF MS system (1.56 ± 0.02 versus 2.24 ± 0.02 for the 5 equivalent and 3.99 ± 0.09 versus 4.43 ± 0.01 for the 10 equivalent, respectively). Increased DAR value accuracy can be obtained for the higher biotin-load when using standard ESI conditions as opposed to nanoESI native-MS conditions.

Bactericidal efficacies of nebulized non-thermal atmospheric plasma-treated liquids

Objectives. Antimicrobial activities of non-thermal atmospheric plasma-treated liquids on various bacterial and fungal strains in their planktonic and biofilm forms have been widely reported. Since most of the plasma-treated liquids are water based, they might be washed off certain surfaces and they cannot be applied for the infections of respiratory tract. In the present study we have tested antimicrobial activities of plasma-treated N-acetyl cysteine solution (NAC), phosphate buffered saline (PBS) solution and deionized water when nebulized over planktonic forms of E. coli and S. aureus . Methods. Antimicrobial activities of nebulized plasma-treated liquids were evaluated with zone of inhibition test and colony counting assay. Moreover pH of NAC, PBS solution and deionized water were measured before, after plasma treatment and during nebulization since low pH is well known consequence observed in plasma-treated liquids. Results. Our results have revealed that pH of plasma-treated NAC, PBS solution and DIW decreases after plasma treatment consistent with previous reports and does not change during nebulization. Moreover, antimicrobial activity assessment indicates that nebulized plasma-treated NAC shows the strongest antimicrobial activity, which leads complete inactivation of bacteria for 10 3 to 10 6 CFU/ml initial bacterial load and 5-log reduction for 10 7 CFU/ml initial bacterial load on both E. coli and S. aureus . Conclusions. Plasma-treated liquids could retain their antimicrobial activity during nebulization and nebulization could be considered as a future alternative method for delivery of plasma-treated liquids for respiratory tract infections.

Chelator-free radiolabeling of dextran with 68Ga for PET studies

The possibility of 68Ga-dextran complex formation was analyzed in an HEPES-buffered (pH = 4.5) as well as in an alkaline (pH = 9) solution. The result outlined that the alkaline solution was more efficient to achieve a radiolabeling efficiency of 99% for a 5 mg ml−1 dextran solution (10 min, 90 °C). 68Ga-dextran was stable 88, 71 and 86% after 2 h at storage and toward cysteine solution and human serum, respectively. The highest ROI derived %ID g−1 was found within the lungs, followed by the blood, liver, and bladder. Furthermore, a significant uptake was observed in the cartilage tissue. 68Ga-dextran excreted more by the urinary and less by the biliary pathways.

Photobiomodulation therapy in the modulation of inflammatory mediators and bradykinin receptors in an experimental model of acute osteoarthritis.

The objective of this study was to evaluate the effects of photobiomodulation therapy (PBMT) on inflammatory indicators, i.e., inflammatory mediators (TNF-α and CINC-1), and pain characterized by hyperalgesia and B1 and B2 receptor activation at 6, 24, and 48h after papain-induced osteoarthritis (OA) in rats. Fifty-four rats were subjected to hyperalgesia evaluations and then divided randomly into three groups-a control group and two groups OA and OA PBMT group by using laser parameters at wavelength (808nm), output power (50mW), energy per point (4 Joules), power density (1.78W/cm2), laser beam (0.028cm2), and energy density (144J/cm2)-the induction of osteoarthritis was then performed with 20-μl injections of a 4% papain solution dissolved in 10μl of saline solution, to which 10μl of cysteine solution (0.03M). The statistical analysis was performed using two-way ANOVA with Bonferroni's post hoc test for comparisons between the 6, 24, and 48h and team points within each group, and between the control, injury, and PBMT groups, and p < 0.05 was considered to indicate a significant difference. The hyperalgesia was evaluated at 6, 24, and 48h after the injury. PBMT at a wavelength of 808nm and doses of 4J, administered afterward, promotes increase at the threshold of pressure stimulus at 6, 24, and 48h after application and promote cytokine attenuation levels (TNF and CINC-1) and bradykinin receptor (B1 and B2) along the experimental period. We conclude that photobiomodulation therapy was able to promote the reduction of proinflammatory cytokines such as TNF-α and CINC-1, to reduce the gene and protein expression of the bradykinin receptor (B1 and B2), as well as increasing the stimulus response threshold of pressure in an experimental model of acute osteoarthritis.