MICP (microbial induced calcium carbonate precipitation) method shows a huge potential in solving geological and geo-environmental engineering problems, such as liquefaction mitigation, soil remediation, erosion control, slope stabilization and dust control., due to its low-carbon, sustainable and undisturbing character. For the liquefaction mitigation, previous studies showed the remarkable enhancement in cyclic behavior of bio-cemented soils. Little attention has been paid to how soil gradation affects the effectiveness of MICP method in enhancing soil properties, which is one of the key factors of soil mechanical behavior. MICP method has been mainly used in fine sands (mostly <1 mm) with narrow gradation and a certain number of particles smaller than 75 μm. As a result, few papers discussed the application of bio-cementation to soils with larger grains. In addition, the respective contributions of density increase and cementation on cyclic resistance enhancement is still not clear. In this study, a fine sand (0.1–1 mm), a coarser sand (1–5 mm), and a mixture of both, were used, featuring a larger mean size (d50 mm) and uniformity coefficient (Cu) than in most previous studies. In this study, cyclic undrained triaxial tests were performed to analyze the liquefaction potential of loose and dense untreated specimens, and initially loose treated specimens. Results show that sand gradation has a great influence on the cyclic behavior and MICP effectiveness. For all the sands, the MICP treatment gives rise to a significant increase in cyclic resistance and mitigates liquefaction. This enhancement is due to the formation of cementitious precipitates in the pores or on the surface of particles, which creates (1) bridges between particles and/or change in grain characteristics (i.e., size, roughness, angularity), and (2) an increase in density. For sands with various gradations, these two effects differ in magnitude: in the coarsest sand, the effect of density increase appears to be predominant whereas, in the finest and medium sands, the formation of bonds seems to play the major part. These results are important to evaluate the possibility of using this method to improve cyclic resistance in various sands, and to compare the treatment effect with other techniques.
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