Responsive Curcumin Research Articles

Synthesis of curcumin and indocyanine green co-loaded PLLA microparticles via solution-enhanced dispersion using supercritical CO2 for chemo-photothermal therapy of osteosarcoma

The therapy of tumor recurrence resulting from surgical resection of osteosarcoma is still a significant challenge for clinicians. In this study, we designed curcumin (CM) and indocyanine green (ICG) co-loaded poly(L-lactic acid) (PLLA) (PLLA-CM-ICG) composite microparticles, combining photothermal therapy with chemotherapy to enhance osteosarcoma therapy synergistically. PLLA-CM-ICG microparticles of sizes of (838.3 ± 5.4) nm were prepared by solution-enhanced dispersion by a supercritical CO2 process. ICG in the PLLA-CM-ICG microparticles was more stable than free ICG. The PLLA-CM-ICG microparticles displayed pH- and near-infrared (NIR)-responsive CM release behavior. Moreover, NIR irradiation improved the anticancer activity of the PLLA-CM-ICG microparticles at a concentration of 250 μg/mL on MG-63 cells. Therefore, PLLA-CM-ICG microparticles could be employed as promising drug delivery systems for synergic photothermo-chemotherapy in osteosarcoma treatment.

Engineering PDA-coated CM-CS nanoparticles for photothermo-chemotherapy of osteosarcoma and bone regeneration

After an osteosarcoma excision, recurrence and bone defects are significant challenges for clinicians. Combination therapy based on a nanoparticle-based drug delivery system shows great promise in osteosarcoma therapy. In this study, we designed a multifunctional chitosan (CS) nanoparticle, which combined photothermal therapy (PTT) with chemotherapy to synergistically enhance osteosarcoma therapy and bone repair. Curcumin (CM) loaded CS nanoparticles were prepared through ionotropic gelation method and were further functionalized with polydopamine (PDA) coating to form CS-CM-PDA nanoparticles (CCPNPs). The CCPNPs displayed pH- and near-infrared (NIR)- responsive CM release behavior. In vitro anti-cancer results indicated that CCPNPs possessed a long-term stable anti-cancer effect compared to pure CM. The presence of the NIR irradiation combined with CCPNPs resulted in a dramatic decrease in MG-63 osteosarcoma cell viability in 10 min compared with the pure CCPNPs at the concentration of 500 μg/mL. Meanwhile, in vitro study demonstrated that CCPNPs induced MG-63 cell apoptosis via downregulating the expression of Bcl-2 and upregulating the expression levels of Bax and Caspase-3. Besides, CCPNPs could positively induce the proliferation of MC3T3-E1 osteoblast cells in vitro. These results suggested that CCPNPs with bifunctional osteosarcoma therapy and bone repair may be an excellent candidate for local cancer therapy and bone regeneration.

PH-sensitive molecularly imprinted polymer based on graphene oxide for stimuli actuated controlled release of curcumin

A new pH responsive curcumin (CUR) imprinted polymer as drug carrier and selective adsorbent of CUR based on graphene oxide (GO@CUR-MIP) was synthesized via copolymerization of acrylic acid (AA) and acrylated β-cyclodextrin (β-CD) as functional monomers, synthesized cross linker and template of CUR. In this study, a four functionalized cross linker was synthesized using tetraethyl orthosilicate (TEOS) and hydroxyethylmethacrylate (HEMA). Vinyl group modified graphene oxide (GO) was prepared through several step of modification and used as surface of polymerization in order to increase the drug loading capacity of MIP. The products were characterized using Fourier transform infrared spectroscopy (FT-IR), thermo gravimetric analysis (TGA), X-ray powder diffraction (XRD), dynamic light scattering (DLS) and scanning electron microscope (SEM) methods. The batch adsorption experiment showed the high adsorption capacity and selectivity of GO@CUR-MIP nanocomposite toward template even in the presence of other structural analogues. The adsorption mechanism was investigated through adsorption kinetics and adsorption isotherm models. The in-vitro release of CUR from pH responsive GO@CUR-MIP nanocomposite was also evaluated in different pH that confirmed the ability of controlled and sustained release of CUR in response to environmental pH changes as stimulus. The pH responsiveness of the GO@CUR-MIP nanocomposite leaded to well-targeted drug delivery potential of the system while β-CD/CUR inclusion complex improved drug interaction with the nanocomposite. The overall results demonstrated the potential of this novel synthesized system in separation and drug delivery process.