BACKGROUND AND AIM: Accumulating epidemiological evidence indicates that high ambient temperatures increase the risk of death. This study aimed to examine the optimum temperatures in Belgium that correspond to minimum mortality, and quantify the relative risks of mortality from all natural causes, and cause-specific causes due to hot and cold non-optimum temperatures. METHODS: A time-stratified case-crossover design fitted with a conditional quasi-Poisson regression model and distributed lag non-linear models were applied to estimate city-specific temperature-mortality associations in nine main Belgian cities, and a random-effect meta-analysis was conducted to pool the estimates. Over the period 2010-2015, a total of 307,859 deaths from natural causes were included. Separate analyses were performed for cause-specific mortality (ischemic heart disease, cerebrovascular, other cardiovascular, chronic obstructive pulmonary disease, other respiratory, and other natural). Analyses were controlled for relative humidity, holidays, long-term and seasonal trends. RESULTS:Considering a cumulative effect of temperature over 21 days before death, both heat and cold were associated with increased mortality risks from all causes. Relative to the minimum mortality temperature (23.1°C, 86.3th centile of the temperature distribution), the pooled estimate of the mortality risk for cold temperature (at -1.7°C, the 1st centile) was 1.32 (95% CI: 1.21-1.44) and 1.21 (95% CI: 1.08-1.36) for hot temperature (at 31.3°C, the 99th centile). When considering a cumulative effect of temperature over 7 days before death, the effect was lower for cold temperature (1.04, 95% CI: 1.00-1.09) but higher for hot temperature (1.33, 95% CI: 1.25-1.41). Adjustment for air pollutants did not change the results. The shapes of the cause-specific related mortality showed various patterns with a clear effect of heat on mortality due to ischemic heart diseases. CONCLUSIONS:This nationwide study provides a comprehensive picture of the non-linear associations between hot and cold temperatures related mortality from cause-specific and all natural causes, in Belgium. KEYWORDS: mortality,temperature,multi-city analysis,causes of death,dlnm
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