Cumulative Changes Research Articles

Immediate effects of propofol on mood: a randomized comparison of two doses in a cohort with depression.

The intravenous anesthetic propofol is known to induce positive mood effects during routine clinical use, suggesting it might be repurposed as an antidepressant, but also raising concerns about abuse potential. How propofol's acute effects vary by dose and with repeated infusions is unknown. This exploratory analysis aimed to (1) compare the immediate mood effects of propofol administered at two different doses, (2) describe how those mood effects change with repeated infusions, and (3) evaluate whether acute mood improvement predicts later antidepressant response. Twenty-four adults with moderate-to-severe treatment-resistant depression were randomized into two dosing groups. Six low- or high-dose propofol infusions were administered under blinded conditions over a two-week period. Self-reported mood states were recorded before and after each infusion using the Positive and Negative Affect Schedule (PANAS-X). Abuse potential was evaluated with the Drug Effects Questionnaire (DEQ-5). At the first infusion, propofol induced acute improvements in PANAS-X Sadness, Fear, Joviality, and Serenity scales (p < 0.002), independent of dose. Over the series of six infusions, acute changes in Sadness, Fear, and Joviality, but not Serenity, diminished with infusion number (p < 0.002). The DEQ-5 "want more" rating decreased across infusions (p = 0.002). Changes in PANAS-X scales with the first infusion did not predict later improvement in depression severity (p > 0.05). Cumulative changes in mood states observed with repeated infusions suggest that propofolengages adaptive mechanisms in mood circuitry. Subjective responses with repeated infusions do not indicate increasing potential for abuse in this patient population.

The Effect of Vitamin D Supplementation with or without Calcium on Vitamin D Epimer and Metabolites.

A possible role of vitamin D epimers and metabolites in the measurement and response to treatment of vitamin D has been reported recently. Furthermore, the influence of underlying vitamin D receptor (VDR) genetic polymorphisms which have been linked to diseases such as obesity remains unclear. We therefore aimed to examine the influence of vitamin D3 and calcium supplements on vitamin D epimer and metabolite concentrations in subjects with and those without vitamin D receptor (VDR) gene polymorphisms. A total of 277 participants who were part of a randomized intervention trial of vitamin D3 and calcium or a placebo for 6 months had clinical and anthropometric assessments. Blood samples were taken for measurements of vitamin D, epimers and metabolites of vitamin D, four vitamin D receptor gene polymorphism SNPs, namely, BsmI, FokI, TaqI, and ApaI, metabolic and inflammatory markers, and related biochemical variables. Repeated-measures analysis of variance was used to assess the between-group difference in cumulative changes in vitamin D epimers and metabolites at 6 months after adjusting for the presence of the 4 VDR genotypes and allele gene polymorphisms. Overall, 277 participants, with a mean (±SD) age of 41 ± 12 and 204 (74%) of whom were female, were included in the study. We found no statistically significant differences in vitamin D metabolites or (epimers) between male and females or younger subjects compared to those over 40 years of age except in 7C4 BL (p < 0.05). There was a statistically significant difference in 1,25(OH)2D3 concentrations between subjects with and those without genotypes AG and the allele G SNP2_Taql VDR gene polymorphism. Vitamin D3 concentrations were also significantly lower in subjects with the CC SNP3_Apal gene polymorphism compared to those without the CC SNP3 gene. No statistically significant effects were seen on vitamin D epimers and metabolites concentration in response to supplements before or after adjusting for the presence of the 4 VDR genotypes and allele gene polymorphisms. The CC SNP3 gene had statistically significant influence on vitamin D3 levels. Vitamin D and/or calcium supplements, however, had no effects on vitamin D epimer and metabolite concentration before or after adjusting for the presence of the 4 VDR genotypes and alleles.

Association Between Cumulative G-force Exposure and Cervical Spine Degenerative Changes.

Fighter pilots work in a unique environment and are subject to high G-force loads under simultaneous head movements. Neck pain is reported to be a common health problem among fighter pilots leading to time lost flying and flight duty limitations. The present study aimed to find out if differences in early military flight career G-force exposure levels could increase the risk for degenerative changes in the cervical spine. The study population consisted of 56 20-year-old Finnish Air Force male fighter pilot cadets, who underwent MRI of the cervical spine at baseline and after 5 years. During follow-up, the G-force exposure was measured using the individual Fatigue Index (FI) recordings. The FI data were collected from each flight with BAE Hawks and is determined by the number of times certain levels of G-forces are exceeded during the flights. The incidence or progression of each degenerative change was compared to individual FI values using Pearson correlation coefficients. The pilots flew on average 220 (±21) hours with high performance aircraft during follow-up, resulting in an average FI of 1.98 (±0.47). A statistically significant progression was found in intervertebral disc (IVD) degeneration sum score with no correlation to corresponding FI values. A statistically significant increase was also found in the prevalence of IVD herniations with a negative correlation with FI values. Other degenerative cervical spine changes did not progress or did not correlate with corresponding FI values. The prevalence of IVD degeneration and IVD herniations increases in the early phases of fighter pilots' career. Only the incidence of IVD herniations correlated with FI values and the correlation was negative. The negative correlation may be attributed to avoidance behavior due to neck pain, which was not measured in our study, or other unmeasured confounding factors. This was the first study to compare individual G-force exposure levels to high-quality MRI data over a follow-up of several years. Finnish fighter pilots are known to report increasing cervical symptoms during the Hawk training phase, but longer follow-up periods are likely needed to determine the association between G-force exposure levels and cervical degenerative changes.

Study on the Effect of Liquefiable Overburden Foundations of Rockfill Dams Based on a Pore Pressure Model

China’s southwestern region boasts abundant hydropower resources. However, the area is prone to frequent strong earthquakes. The areas surrounding dam sites typically have deep overburden, and the liquefaction of saturated sand foundations by earthquakes poses significant safety risks to the construction of high dams in the southwest. The effects of liquefaction and reinforcing measures on the foundations of rockfill dams on liquefiable overburden under seismic action are currently the subject of somewhat unsystematic investigations. The paper utilizes the total stress and effective stress methods, based on the equivalent linear model, to perform numerical simulations on the overburden foundations of rockfill dams. The study explores how factors such as dam height, overburden thickness, liquefiable layer depth, liquefiable layer thickness, ground motion intensity, and seismic wave characteristics affect the liquefaction of the overburden foundations. Additionally, it examines how rockfill dams impact the dynamic response, considering the liquefaction effects in the overburden. The results show that although the total stress method, which ignores the cumulative evolution of pore pressure during liquefaction, can reveal the basic response trend of the dam, its results in predicting the acceleration response are significantly biased compared to those of the effective stress method, which comprehensively considers the cumulative changes in liquefaction pore pressure. Specifically, when the effect of soil liquefaction is considered, the predicted acceleration response is reduced compared to that when liquefaction is not considered, with the reduction ranging from 4% to 30%; with increases in the thickness and burial depth of the liquefiable layer, the effective stress method considering liquefaction significantly reduces the predicted peak acceleration; the effect of liquefiable soil on the attenuation of the speed response is more sensitive to the low-frequency portion of the seismic wave. The study’s findings are a significant source of reference for the planning and building of rockfill dams on liquefiable overburden.

Allostatic load in rat model: An efficient tool for evaluating and understanding aging.

Aging and age-related diseases are an ever-increasing social and public health problem. Allostatic load (AL) shows great potential as an interdisciplinary tool for assessing the aging of human beings but as yet lacks investigation in animal models which is our study focus at. Here a continuous study of AL was conducted on naturally aging rats. Blood samples were collected from the rats at ages of 5, 8, 14, 18, and 21 months. Dozens of blood biochemical indicators, including serum corticosterone, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, triglycerides, C-reactive protein, interleukin-6, 25-hydroxyvitamin D, free fatty acid, CD3+ T cell count, CD4+/CD3+ T cell ratio, CD8+/CD3+ T cell ratio, and CD3/4/8+ T cell apoptosis, were determined. AL was scored from those indicators, and we found that AL score gradually increased with age. AL can reliably reveal the cumulative and systemic changes in aging. Geriatr Gerontol Int 2024; 24: 1077-1084.

Combined metabolomics and transcriptomics reveal the secondary metabolite networks in different growth stages of Bletilla striata (Thunb.) Reichb.f.

Bletilla striata (Thunb.) Reichb.f. (B. striata) is a traditional Chinese medicinal herb. B. striata polysaccharides (BSP), stilbenes and 2-isobutyl malic acid glucosoxy-benzyl ester compounds are the main active ingredients in B. striata. However, there is limited report on the changes of medicinal components and their biosynthesis regulation mechanisms in the tubers of B. striata at different stages. The tubers of B. striata were collected during the flowering period, fruiting period, and harvest period to determine the total polysaccharide content using the phenol sulfuric acid method. The changes in secondary metabolites in the tubers at these stages were analyzed by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS), and transcriptomics was conducted for further exploration of their biosynthetic pathways. The BSP content gradually increases from the flowering period to the fruiting period as the tubers develop, reaching its peak, but subsequently decreases at harvest time, which may be associated with the germination of B. striata buds in later stage. A total of 294 compounds were identified in this study. Among them, a majority of the compounds, such as 2-isobutyl malate gluconoxy-benzyl ester, exhibited high content during the fruit stage, while stilbenes like coelonin, 3'-O-methylbatatasin III, and blestriarene A accumulated during the harvesting period. The transcriptome data also revealed a substantial number of differentially expressed genes at various stages, providing a partial explanation for the complex changes in metabolites. We observed a correspondence between the expression pattern of GDP-Man biosynthesis-related enzyme genes and cumulative changes in BSP. And identified a positive correlation between 9 transcription factors and genes associated with polysaccharide biosynthesis, while 5 transcription factors were positively correlated with accumulation of 2-isobutyl malate gluconoxy-benzyl ester compounds and 5 transcription factors exhibited negative correlated with stilbene accumulation. It is imperative to determine the appropriate harvesting period based on the specific requirements of different active ingredients and the accumulation patterns of their metabolites. Considering the involvement of multiple transcription factors in the biosynthesis and accumulation of its active ingredients, a comprehensive investigation into the specific regulatory mechanisms that facilitate high-quality cultivation of B. striata is imperative.

Towards an integrative understanding of British Columbia’s Nechako Watershed: Connecting knowledge systems to strengthen understanding of climate change, watershed security, health and well-being

Understanding of upstream and downstream dynamics of continental river basins demands attention to the influence of important tributaries and watersheds. This is exemplified by the 47,200 km2 Nechako Watershed, the second largest sub-watershed of British Columbia’s Fraser River Basin. Although the Nechako (derived from the Indigenous Dakelh word meaning “big river”) is recognised for its ecological, societal and cultural importance, attention to this sub-watershed has often been overshadowed by a focus on the iconic Fraser River. This paper examines insights from a purposeful response to this gap, whereby a team of researchers has worked together to strengthen understanding of cumulative stressors and changes in the Nechako, focusing on climate change and water security, sediment sources and quality, and health and well-being dynamics within the Nechako Watershed. Lessons learned from a decade of this collaboration are presented, reflecting on the Nechako Watershed’s past, present, and future through the lens of a unique case study of interdisciplinary research. Emerging research and knowledge exchange partnerships are highlighted along with growing concerns for the Nechako’s keystone aquatic species including three species of Pacific salmon and the endangered Nechako white sturgeon. Drawing on the natural, social and health sciences, we examine strengths and challenges of connecting research across interrelated watershed security issues ranging from climate change, landcover disturbances (e.g., wildfires, mountain pine beetle outbreaks and forest harvesting), land use changes (e.g., expansion of Vanderhoof’s agricultural belt), and the far-reaching impacts of the damming of the Nechako River mainstem in the 1950’s. Our paper brings necessary attention to these and other influences on waterways, landscapes and communities of the Nechako Watershed, highlighting new research opportunities arising among diverse knowledges and disciplines, and the ongoing collaborative effort required to address emerging challenges for the Nechako and wider Fraser River Basin, with consequences for current and future generations.

Cumulative Changes in Minimum Snow/Ice Extent over Canada and Northern USA for 2000–2023

The Minimum Snow/Ice (MSI) extent is an important climate and environmental indicator related to surface hydrology and freshwater resources. Variations in the MSI extent over the four first-order regions (1-4) included in the Randolph Glacier Inventory (RGI) for Canada and the Northern USA were analyzed in this study using an improved land-water mask to better discriminate landfast ice in the high Arctic coastal zone. The analysis utilized warm season snow/ice probability maps derived from MODIS clear-sky composite imagery at a 250-m spatial resolution. Results showed statistically significant declining trends in the minimum snow/ice extent with the average value of the slope for the entire area (-1,177.7 ± 362.8) km2/yr. The variations in MSI extent are well correlated with surface air temperature. The correlation coefficients are statistically significant, ranging from −0.79 to −0.82. The detailed analysis revealed a reduction of the area with snow/ice probability 100% over the RGI glaciated zones, while areas with lower probability values were increasing in size, which may be interpreted as an indication of general glacier shrinkage over the study area.

Establishment of debris index evaluation criteria and comparison of index effects

This paper discusses the processes which should be taken to establish debris indices that assess the orbital environmental impact of spacecraft or missions in orbit. The first process is establishing a methodology and criteria for evaluating an impact on the orbital environment. This study proposed an assessment method to evaluate the cumulative environmental changes during an evaluation period. It evaluated the impact of an uncontrolled LC mission abandoned in orbit. This study used Near-Earth Orbital Debris Environment Evolutionary Model (NEODEEM), an orbital environmental evolutionary model that Kyushu University and JAXA developed. The second process is the debris index formulation. This study considered four steps to establish the debris index. To reveal the establishing process of a debris index, this study used debris indexes as candidates which consider expected number of fragments generated from an object. The calculated debris index values were compared to the impact on the environment assessed by the evolutionary model to see if there was any correlation. Further, this study conducted numerical simulations of un-controlled large-constellation satellites abandoned in low earth orbit in different scenarios, and of active debris removals of large intact objects by NEODEEM. This paper discusses the applicability of proposed environmental assessment methods and the debris index.

A temporal difference matrix for historical cumulative change detection in time series PolSAR data

To detect historical cumulative changes in land cover, this study introduces a novel time series PolSAR change detection method. The objective is to address the inefficiency of traditional detection methods that use multiple pairwise comparisons, as well as to alleviate false positives and false negatives caused by the insufficient utilization of polarization and spatial context information in previous methods. The proposed method initially constructs a temporal difference matrix for time series PolSAR images and computes the difference image (DI) using the maximum eigenvalue of this matrix to depict the cumulative changes over time. Subsequently, the DI is segmented using an MRF-based image segmentation method with a limited amount of supervised information, yielding the cumulative change binary map. The efficacy of the proposed method is validated using three time series datasets covering different scenes acquired by different sensors, namely GaoFen3, Radarsat2 and Sentinel-1. The experimental results demonstrate that the method effectively characterizes cumulative changes in the time series while simultaneously considering disparities in radar backscatter intensity and polarization correlation. As a result, it significantly reduces erroneous and missed change detections and surpasses the existing methods by achieving the highest Recall, F1-score, IOU, Kappa, and overall accuracy. Furthermore, the DI calculation method serves as a versatile change descriptor applicable not only for capturing cumulative changes in time series but also for describing changes between dual temporal images.

Compounding Effects of Aging and Sex on Cerebrovascular Endothelial Mitochondria

Despite the increasing burden of age-related neurological diseases such as vascular cognitive impairment and dementia or Alzheimer’s disease, the underlying mechanisms of these pathologies are not clear. Nonetheless, structural and functional changes in the microvasculature represent probable pathological aspects of these age-associated diseases. The silent and cumulative changes in endothelial mitochondria with subsequently compromised cerebral vascular function are thought to play a central role in neurological disease development. We tested the hypothesis that mitochondrial morphological changes in endothelial cells facilitate the distinct aging- and sex-related differences in the cerebral microvascular proteome and energetics. Mito-dendra2 (mt-D2) labeled endothelial mitochondria were imaged in vivo via a cranial window using two-photon microscopy at 875 nm in young and old, male and female, mito-Dendra2 mice (YM, YF, OM, and OF, respectively). Mt-D2 associated signal was analyzed in 3D stacks using the Huygens Pro CE 23.04.0p3 object analyzer (160 images, originated from 4-5 mt-D2 mice/groups). We also measured mRNA copy numbers of mitochondrial fission and fusion genes in isolated, cortical microvessels (&lt;70 μm) from YM, YF, OM, and OF C57Bl/6J mice (n=7-11/age/sex) using ddPCR. Kruskal-Wallis test with Dwass-Steel-Critchlow-Fligner post-hoc comparisons were used to compare mt-D2 signal among YM, YF, OM, and OF, whereas Mann-Whitney U test determined changes across age and sex strata. p&lt;0.05 was considered statistically significant. mRNA copy numbers were normalized to housekeeping genes (GAPDH, β-actin, and HPRT-1) and compared with one-way ANOVA based on data distribution. Analysis of four object parameters revealed that object surface was smaller in old vs. young that was significant in YM vs. OM. We found significantly greater corrected lateral width in OM vs. YM, OF vs. YF, and in YF vs. YM. The lateral aspect ratio was significantly greater in YM vs. OM, in YF vs. OF, as well as in YM vs. YF. Accordingly, the axial sphericity of the principal box was significantly greater in OM vs. YM, in OF vs. YF, and in YF vs. YM. Normalized mRNA copy numbers of mitochondrial fission and fusion genes were not statistically different in our samples for DRP-1, Fis-1 or Mfn-1/2, Opa-1, respectively. These results suggest that aging driven morphological differences, such as the shortening of endothelial mt-D2 signal are, at least in part, facilitated by altered fission/fusion protein abundances and/or activity rather than transcriptional changes in our samples. Although we are still analyzing other morphological parameters, by assessing the effect sizes and their physiological relevance, we postulate that these morphological differences contribute to the vulnerability of the aged cerebral microvasculature to injury. AG083567, 3P30GM145498-01, U54GM104940, HL148836. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

THE RELATIONSHIP OF ORAL HEALTH AND QUALITY OF LIFE FOR ELDERLY AT THE TRESNA WERDHA NIRWANA SOCIAL ORGANIZATION PURI SAMARINDA

Introduction: Elderly is the final stage of the aging process, usually followed by cumulative changes in human that create changes in the oral cavity as well. Oral Health plays a crucial role in general health and quality of life. Material and Methods: : This research used analytical observational study with cross-sectional design, using purposive sampling as its sampling method. The respondents were 81 elderly people. This research used oral health Levin questionnaire and Oral Health Impact Profile (OHIP-14). The data from this research were analyzed using Spearman correlation test. Results and Discussions: The correlation between dental and oral health and quality of life showed significant with the p value &lt;0.001 and correlation coefficient R= 0.657. Conclusion: There was a significant correlation between dental and oral health and the quality of life of elderly people in Tresna Werdha Nirwana Puri Samarinda Nursing home.

Stress triggering and future seismic hazards implied by four large earthquakes in the Pamir from 2015 to 2023 revealed by Sentinel-1 radar interferometry

SUMMARY The Mw 6.8 Murghob earthquake is the third earthquake in an Mw 6.4+ sequence occurring in the Pamir initiated by the 2015 Sarez Mw 7.2 earthquake. It is of great significance to investigate their interactions and to assess future seismic hazards in the region. In this paper, we use Sentinel-1 radar interferometric data to retrieve coseismic deformation, invert for the slip distributions of the four events, and then investigate their interactions. The cumulative Coulomb failure stress changes (ΔCFS) suggest that the 2023 Murghob earthquake was promoted by the three prior earthquakes in the sequence. Pre-stress from historical earthquakes is a key factor in explaining the triggering mechanism of the two 2016 Mw 6.4+ earthquakes. Stress loading and unloading effects on major faults in the region indicate that future attention should be paid in (1) the segment of the Sarez-Karakul fault north of the Kokuibel Valley, (2) the segment of the Sarez-Murghab thrust fault west of the Sarez-Karakul fault and (3) the east segments of the Pamir thrust fault system, all with a large positive ΔCFS.

Temperature description and heat extraction optimization of enhanced geothermal system via failure volume ratio

It is difficult to quantitatively measure the temperature characteristics to evaluate the heat extraction in Enhanced Geothermal Systems (EGS). This study aims to define a new evaluation index called failure volume ratio to describe the temperature distribution in rocks and optimize heat extraction. In the established three-dimensional thermal-hydrologic coupling model, the cumulative heat production and production temperature are interpreted and predicted by the proposed index. A set of orthogonal experiments is designed to quantitatively find the key factors affecting EGS. Results show that the failure volume ratio can provide a more rational and concise explanation for changes in the temperature and heat of production during EGS heat extraction. The ratio when the production temperature begins to decrease reflects how much heat has been extracted at the maximum production temperature. In a given model, we only need to calculate the failure volume ratio which reflects both cumulative heat production and production temperature changes, which is useful for a combined system of above-ground power generation and underground heat extraction. Based on the analysis of variance, the injection flow has the greatest impact on EGS. Its optimal value in this model is 80 kgs−1. This study provides a quantitative tool for reservoir evaluation and geothermal system optimization.

Time Thresholds for Using Pressure Reactivity Index in Neuroprognostication for Patients With Severe Traumatic Brain Injury.

Severe traumatic brain injury (sTBI) represents a diffuse, heterogeneous disease where therapeutic targets for optimizing clinical outcome remain unclear. Mean pressure reactivity index (PRx) values have demonstrated associations with clinical outcome in sTBI. However, the retrospective derivation of a mean value diminishes its bedside significance. We evaluated PRx temporal profiles for patients with sTBI and identified time thresholds suggesting optimal neuroprognostication. Patients with sTBI and continuous bolt intracranial pressure monitoring were identified. Outcomes were dichotomized by disposition status ("good outcome" was denoted by home and acute rehabilitation). PRx values were obtained every minute by taking moving correlation coefficients of intracranial pressures and mean arterial pressures. Average PRx trajectories for good and poor outcome groups were calculated by extending the last daily averaged PRx value to day 18. Each patient also had smoothed PRx trajectories that were used to generate "candidate features." These "candidate features" included daily average PRx's, cumulative first-order changes in PRx and cumulative second-order changes in PRx. Changes in sensitivity over time for predicting poor outcome was then evaluated by generating penalized logistic regression models that were derived from the "candidate features" and maximized specificity. Among 33 patients with sTBI, 18 patients achieved good outcome and 15 patients had poor outcome. Average PRx trajectories for the good and poor outcome groups started on day 6 and consistently diverged at day 9. When targeting a specificity >83.3%, an 85% maximum sensitivity for determining poor outcome was achieved at hospital day 6. Subsequent days of PRx monitoring showed diminishing sensitivities. Our findings suggest that in a population of sTBI, PRx sensitivities for predicting poor outcome was maximized at hospital day 6. Additional study is warranted to validate this model in larger populations.

The Coping Strategies and Cumulative Changes in Intensive Care Unit Nurses after Experiencing Professional Grief: A Hermeneutic Phenomenological Study

Background. Compared to nurses in other hospital departments, intensive care unit (ICU) nurses have more frequent exposure to patient deaths, potentially rendering them more susceptible to experiencing professional grief following patient fatalities. Objective. To explore the coping experiences of ICU nurses following their encounter with professional grief. Methods. This study utilized a qualitative research design based on Heideggerian phenomenology. A purposive sample of 18 ICU nurses was selected from six tertiary hospitals in Guangdong Province, China. Individual semistructured interviews were audio-recorded and transcribed verbatim. The transcribed texts were analyzed using interpretative phenomenological analysis. Results. Two main themes emerged: (1) short-term dual coping with professional grief and (2) long-term cumulative changes from professional grief. These themes reflect the dynamic coping processes experienced by ICU nurses amidst frequent encounters with loss and grief during their clinical work. Conclusions. ICU nurses employ both adaptive and maladaptive coping strategies to address professional grief, significantly impacting their personal and professional well-being. It is advisable to offer targeted education and organizational support systems for ICU nurses to promote positive cumulative growth when they repeatedly encounter patient deaths.

Ecosystem CO2 Exchange and Its Environmental Regulation of a Restored Wetland in the Liaohe River Estuary

Coastal wetlands are important carbon sinks， and they contribute to reducing the effects of global warming. This study used the eddy covariance method to detect the CO2 flux in the restoration wetland of the Liaohe River estuary in 2021 and investigate the characteristics of ecosystem CO2 exchange and its environmental control factors. The aim was to assess the carbon source/sink capacity of salt marshes in the restored area and to provide data support and theoretical basis for evaluating the effectiveness of ecological restoration projects. The study revealed "U" curves in spring and autumn， "V" curves in summer， and horizontal lines in winter for the average daily variation curve of net ecosystem CO2 exchange （NEE） in the restored area. Its carbon sink efficiencies were -40.06， -63.62， 2.33， and 34.43 g·m-2 in the spring， summer， autumn， and winter， respectively. In the restored area， the daily cumulative variation in NEE was "V" shaped， and the monthly cumulative changes in NEE， ecosystem respiration （Reco）， and gross primary productivity （GPP） were obviously different. Photosynthetically active radiation （PAR） was an important regulation factor of daytime NEE in the restored area in 2021， and they displayed a rectangular hyperbolic relationship. PAR could explain 53% of the variation in the daytime NEE. Air temperature （Ta） was the main control factor of Reco，night， and there was an exponential relationship between them. When Ta &lt; 5.5 ℃， the temperature sensitivity of ecosystem respiration （Q10） was 2.19， and Ta could explain 42% of the variation in the Reco，night； when Ta ≥ 5.5 ℃， the Q10 was 1.81， and Ta could explain 51% of the variation in the Reco，night. Additionally， there were significant linear negative correlations between NEE and both soil water content （SWC） and vapor pressure deficit （VPD）， whereas NEE was not significantly correlated with soil temperature （Ts） or relative humidity （RH）. In 2021， the restored wetland in the Liaohe River estuary acted as a CO2 sink， and the total net carbon sequestration was -66.89 g·m-2. The restored salt plays a role as an important carbon sink and has long-term carbon sequestration potential.

The shape of the change: Cumulative and incremental changes in daily mood during mobile-app-supported mindfulness training.

Understanding of the exact trajectories of mood improvements during mindfulness practice helps to optimize mindfulness-based interventions. The Mindfulness-to-Meaning model expects mood improvements to be linear, incremental, and cumulative. Our findings align with this expectation. We used multilevel growth curve models to analyze daily changes in positive mood reported by 190 Polish participants during 42 days of a mobile-app-supported, mindfulness-based intervention. The daily positive mood increased among 83.68% of participants. Participants who started the training reported worse mood improved more and faster than participants with better mood at the baseline. Dispositional mindfulness and narcissism - individual difference variables associated with high vs. low emotion regulation ability, respectively - were not associated with mood improvement trajectories. A small group of participants (16.32%) showed a steady decline in positive mood during the intervention. The results underscore the importance of a more comprehensive understanding of individual variability in benefiting from mindfulness-based interventions.

SeedMatchR: identify off-target effects mediated by siRNA seed regions in RNA-seq experiments.

On-target gene knockdown, using siRNA, ideally results from binding fully complementary regions in mRNA transcripts to induce direct cleavage. Off-target siRNA gene knockdown can occur through several modes, one being a seed-mediated mechanism mimicking miRNA gene regulation. Seed-mediated off-target effects occur when the ∼8 nucleotides at the 5' end of the guide strand, called a seed region, bind the 3' untranslated regions of mRNA, causing reduced translation. Experiments using siRNA knockdown paired with RNA-seq can be used to detect siRNA sequences with off-target effects driven by the seed region. However, there are limited computational tools designed specifically for detecting siRNA off-target effects mediated by the seed region in differential gene expression experiments. SeedMatchR is an R package developed to provide users a single, unified resource for detecting and visualizing seed-mediated off-target effects of siRNA using RNA-seq experiments. SeedMatchR is designed to extend current differential expression analysis tools, such as DESeq2, by annotating results with predicted seed matches. Using publicly available data, we demonstrate the ability of SeedMatchR to detect cumulative changes in differential gene expression attributed to siRNA seed region activity. SeedMatchR is available on CRAN. Documentation and example workflows are available through the SeedMatchR GitHub page at https://github.com/tacazares/SeedMatchR.

Impact of different doses of esketamine on the incidence of hypotension in propofol-based sedation for colonoscopy: a randomized controlled trial.

Hypovolemia is common in colonoscopy due to fasting and bowel preparation, and propofol itself can reduce systemic vascular resistance, resulting in relative hypovolemia. Therefore, hypotension is not a rare event during propofol-based sedation for colonoscopy. Our objective was to explore the efficacy of esketamine as a sedative adjuvant in reducing the incidence of hypotension during colonoscopy. This was a prospective randomized trial. The trial was registered with the Chinese Clinical Trial Registry (ID: ChiCTR 2100047032). We included 100 eligible patients who planned to receive a colonoscopy and randomly divided them into 4 groups with 25 patients in each group, which were propofol 2 mg/kg (Group P), propofol 1 mg/kg with esketamine 0.2 mg/kg (Group E1), propofol 1 mg/kg with esketamine 0.3 mg/kg (Group E2), and propofol 1 mg/kg with esketamine 0.4 mg/kg (Group E3). The hemodynamic and respiratory parameters were documented at various times during the procedure, including the patient's entry into the endoscopic room (T0), the induction of sedation (T1), the insertion of the colonoscope (T2), the removal of the colonoscope (T3), and the awakening of the patient (T4). The primary outcome was the incidence of hypotension. Secondary outcomes were cardiovascular side effects other than hypotension, incidence of hypoxia, cumulative changes in cardiovascular and respiratory parameters, total propofol dosage, anesthesia recovery time, and satisfactory levels of both patients and endoscopists. The incidence of hypotension in Group E1 (16%), Group E2 (16%), and Group E3 (12%) was significantly lower than in Group P (60%), with p values 0.003, 0.003, and <0.001 respectively. The cumulative changes in diastolic blood pressure and mean arterial pressure in Groups E1, E2, and E3 were significantly higher than in Group P (p = 0.024, p < 0.001, p = 0.006, respectively). Cumulative changes in systolic blood pressure in Group E3 were significantly higher than those in Group P (p = 0.012). The respiratory-related parameters were not statistically significant. This study showed that the application of 0.4 mg/kg esketamine in propofol-based sedation reduced the incidence of hypotension during colonoscopy while providing satisfactory sedation.