Culture-based Model Research Articles

Agricultural Landscape Model Based on the Culture of the Indigenous People of Tana Toraja, South Sulawesi

Abstract The interaction between humans and their environment forms cultural landscape. One of the cultural landscape in Indonesia is Tana Toraja. The Tana Toraja indigenous people’s way of life refers to the Aluk Todolo religion which regulates the relationships of between human-human, human-God (Puang Matua), and human-environment. The regulation influences their environment, settlement, and agriculture management. Agriculture in Tana Toraja is managed by the community not only for the economic sector but also to ensure food security and preserve traditional cultural activities. This study aims to identify and analyze the elements that make up the agricultural landscape of the Tana Toraja indigenous people, as well as develop a local culture-based agricultural landscape model. The method using in this research was spatial descriptive method by tracing customary manuscripts (literature review), interviewing traditional leaders, and field observations. This research has identified the elements that make up the Tana Toraja agricultural landscape were hills, rivers and springs, forests, rice fields, dryland fields, gardens, fish ponds (gusean), yards, and cattle pens. Integrated agriculture that is carried out based on local customs is paddy-fish integrative farming, rice-livestock integrative farming, agroforestry, and crop-livestock integrative farming. The form of integrated agriculture can be seen at the macro and micro levels and consists of spatial concepts, vegetation concepts, and circulation concepts. At the macro level, which consists of four landform hierarchies in the settlement landscape, which indicates their philosophy of life, they are successively a sacred place (hill), their ancestral home, the general settlement and agricultural areas, and development area for their subsequent offspring.

Implementation of the Angkola Culture-Based Experiential Learning Model in the Technological Era

Implementation of the Angkola Culture-Based Experiential Learning Model in the Technological Era

Culture-based agricultural development model in dragen village, Jaten subdistrict, Karanganyar regency

Background: Agriculture plays an important role in a country's economic development. However, the agricultural sector often faces challenges in sustainable development. This research aims to identify the potential and factors causing agricultural problems and analyse structural transformation in Dagen Village, Karanganyar Regency. Methods: The research method used a descriptive approach with data collection through interviews and literature studies. Results: The results showed that Dagen Village has great agricultural potential, but the lack of community interest in the agricultural sector and farmers' lack of knowledge about modern agricultural techniques are the main obstacles in utilising this potential. In addition, structural transformation is also occurring in Dagen Village, where there is a shift from the traditional agricultural sector to the modern economic sector. Findings: The importance of paying attention to cultural aspects in agricultural development and structural transformation is also highlighted in this study, as culture plays a role in shaping people's mindset and behaviour towards agriculture and economic development. Conclusion: Thus, this research provides insight into the importance of integration between aspects of culture, agriculture, and economic development in the context of rural development.

Effectiveness of the Scientific Inquiry Learning Model Based on Local Malay Cultural Wisdom on Student Learning Interests

The aim to be achieved through research is to see the effect of implementing the Malay Culture-based scientific inquiry learning model on increasing student interest in learning. This study employed a quantitative approach and included 55 students in their third semester, divided into two sample groups. The experimental class (Class A) consisted of 27 students, while the control class (Class B) comprised 28 students.. This research was conducted in the context of the Hydrology course. Data was collected through tests and analyzed using IBM SPSS 23. After implementing the scientific inquiry learning model based on Malay culture and conventional learning, the average pretest score for student interest in learning in the experimental class was 59.77 and after being given treatment using the scientific inquiry learning model, The student's posttest score was 80.55. The results of the independent sample-test showed a significant value of 0.000, where the value was <0.05. Thus, it can be deduced that the use of the Malay Culture-based scientific inquiry learning model has a positive impact in increasing students' interest in learning.

The Relation Between Mathematics Philosophy And Culture-Based Learning Model

The aim of this study is to study the correlation of mathematical philosophy with cultural-based learning models. The method of research used is the method of library research, i.e. conducting studies related to mathematical philosophy, philosophical mathematics learning, culture-based mathematic learning, the connection of mathematically philosopher with ethnomatematics, the relationship of mathematic philosophies with cultural-based learning models. The results of the research have been obtained that the philosofy of maths, the model of learning based on culture, and ethnomathematics have close connections in the context of understanding and teaching of Mathematics. Mathematical philosophy deals with the foundations of mathematical epistemology and ontology, while a culture-based learning model emphasizes the integration of values and cultural contexts in mathematics learning. Ethnomatematics refers to the study of mathematical ways of thinking in a particular culture. By understanding the cultures, traditions, and world views of a particular society, ethnomathematics can help develop more culturally relevant and meaningful learning models. Therefore, the interrelationship between mathematical philosophy, culture-based learning models, and ethnomathematics can create a more holistic and contextual approach to mathematics learning.
 Keywords: Mathematical Philosophy, Cultural Based Learning Model, Ethnomathematics

Character values education model based on School Culture of the Muhammadiyah Leading Primary School in Lemahdadi Yogyakarta

Cases of delinquency start from elementary school age, such as cases of bullying, smoking at an early age, lack of good manners, and various other types of child delinquency. If early childhood delinquency is not immediately addressed, it will have an impact on children's delinquency in adolescence. The aim of this research is to analyze the character values education model based on Muhammadiyah school culture at the Muhammadiyah Lemahdadi Flagship Elementary School, as well as the usefulness of this character values education model. The research method uses descriptive qualitative, primary data for this research was obtained from the school principal, two Ismuba teachers, ten students, and the head of administration. The data processing process using research methods is carried out simultaneously, then continuous data verification is carried out until the problem formulation is answered. The results of the research show that the school culture-based character values education model is reflected in a series of cultures at Muhammadiyah Lemahdadi's flagship primary school, which interprets the combination of strengthening religious values, leadership development, involvement in physical activities, and developing business skills, with the aim of forming good character. strong, good morals, and students' readiness to face future challenges.

Abstract P3060: Modeling Immune Checkpoint Inhibitor Associated Myocarditis In Vitro

Introduction: Immune checkpoint inhibitor-associated myocarditis (ICI myocarditis) is a severe side effect of treating malignancies with immune checkpoint inhibitors. Patients suffering from ICI myocarditis often display an extraordinarily high mortality rate. Establishing a clinically relevant model to address the lack of identifiable risk factors, screening tests, or effective treatments is critically urgent. Hypothesis: Human ICI myocarditis can be accurately modeled in vitro. Methods and Results: We present the first cell culture-based model of ICI myocarditis. We use a novel method to co-culture human iPSC-derived cardiomyocytes (hiPSC-CM), human peripheral blood mononuclear cells, and immune checkpoint inhibitors to recapitulate critical aspects of clinical ICI myocarditis. Using this platform, we show rapid, significant cardiomyocyte necrosis after model initiation. We observe substantial disruption to hiPSC-CM cell morphology and sarcomere structure, and we use powerful multi-electrode array technology to define contractile functions and electrophysiological changes, demonstrating the critical similarities between this cell-based ICI myocarditis model and patient myocarditis. Our model also reveals an increased risk of arrhythmia, recapitulating, in vitro , the primary cause of death in patients suffering from ICI myocarditis. Finally, we demonstrate that this hiPSC-CM-based “model in a dish” can be rescued pharmacologically and identify specific leukocyte populations required for myocarditis development. In summary, we present the first in vitro model of ICI myocarditis and demonstrate its scalability, validity, patient relevance, and reproducibility. Utilizing the model can aid in accomplishing precision medicine objectives and holds significant potential for preventing and treating ICI myocarditis.

Level of Readiness for Implementation of the Independent Curriculum in Senior High Schools in Depok City, West Java

The Independent Curriculum is a curriculum with diverse intra-curricular learning where the content will be optimized so that students have enough time to explore concepts and strengthen competencies. The purpose of this study was to identify and obtain information about the level of readiness of education units in Depok City in implementing the independent curriculum. The research method used is the descriptive qualitative method, which describes the subject the situation, and data obtained during observations and questions so that it becomes useful information and is easily understood by readers. The results of this study indicate that the level of readiness for implementing an independent curriculum in schools in high school education units in Depok City, West Java, is still not optimal. Two of the three schools have not implemented an independent curriculum, although the school has received socialization and understanding related to the independent curriculum. The key to the success of implementing an independent curriculum is good collaboration between school principals and teachers. In addition, the principal as a leader must be able to change the mindset of the Human Resources in the school to want to make changes so that the independent curriculum can be applied. Likewise, in the project of strengthening the profile of Pancasila students, it is still limited and has not led to a local culture-based learning model

Batak Culture-Based Learning Model: Character Building and Improving Learning Outcomes in Pancasila and Citizenship Education

Batak Culture-Based Learning Model: Character Building and Improving Learning Outcomes in Pancasila and Citizenship Education

Pengembangan Model Pembelajaran Inkuiri-Seni Berbasis Google Art and Culture untuk Siswa Sma

Appreciation learning has not taken place optimally with its delivery focusing more on understanding theory, involving less active student participation and using limited learning resources. Based on these conditions, it is considered necessary to develop an appreciation learning model, especially the appreciation of painting works using a more effective learning model. This development research aims to produce an inquiry-art learning model based on Google Arts and Culture. This research uses Research and Development (RnD) research approach. The subjects of this study were grade XI high school students in Kebumen Regency. Data collection techniques are carried out by observation, interviews and using questionnaires. The collected data was analyzed with a combination of qualitative
 
 
 
 
 
 
 
 
 
 
 
 
 
 Misdemeanors, Restorative Justice, Criminality
 
 
 
 
 
 
 
 
 
 
 
 
 
 and quantitative. The resulting product is in the form of a learning implementation plan, learning model syntax, and student activity sheets. The results of the study obtained showed that the development of learning models applied to grade XI high school students showed that students were more enthusiastic and actively involved in the learning process, compared to previous learning. The ability of students to respond in discussion activities and through painting appreciation worksheets is proven to be good, so that the Google Arts and Culture-based inquiry-art learning model is feasible to be applied in appreciation learning in schools.

The French Cultural Tourism: Exploring the Harmonious Coexistence of Tradition and Modernization

Cultural tourism has become the doctrine of tourism development in many countries. Various local commodities have been transformed into tourist attractions to satisfy the curiosity of tourists. Unfortunately, cultural tourism in many destinations tends to be exclusive, where its activities focus on presenting artificial cultural attractions that are packaged for tourists. This article describes the best practice of how France has succeeded in developing a culture-based tourism sector amidst the demands of modernization and industrialization. Through a qualitative descriptive approach, data and information were collected from observation, literacy studies, and interviews. The authors conducted a thematic analysis to portray a culture-based French tourism model. The analysis results conclude that French tourism is firmly supported by a wealth of culture and traditions that have long been rooted in people's lives.

(149) PD.SPHERE, a Novel Three-dimension Cellular Model for Peyronie’s Disease via ‘Microgravity’ Condition: The New Era of in vitro Cell Culture-based Model for PD

Abstract Introduction Peyronie's disease (PD) is a common problem that affects 4-13% of the male population and results in a significant decline in patient and partner quality of life. Currently, in vitro monolayer fibroblast culture and animal models are actively used to study pathogenesis and treatments. However, both models are not a great pathophysiological representative for human PD due to the lack of cellular architecture and limited insight into the PD mechanism. Objective We aimed to develop a three-dimensional (3D) cellular model to reveal the PD pathogenesis mechanism and perhaps use this model as a treatment screening tool. Methods Human PD fibroblasts isolated from PD patients during surgery were cultured in various non-adhesive culture plates under the 'microgravity' condition. Complete media (DMEM+10% FBS+2mM L-glutamine) with and without TGF-β supplement was used to optimize the conditions for the PD.SPHERE, a 3D cell culture model of PD fibroblasts. Molecular probes against nucleus, actin, vimentin (VIM) and smooth muscle actin (SMA) were used to study the morphology and expression of molecular markers. Confocal-microscopic analyses were performed to obtain the morphological snapshots of and molecular data of the PD.SPHERE. Results We successfully developed a 3D cell culture (spheroid) model from human PD fibroblasts with our defined conditions. The PD spheroids were formed individually in each vessel of a 96-well plate coated with a non-adhesive substance. A PD.SPHERE was made in each well in a 96-well plate from three different individuals. Moreover, the size and circularity of spheroids were consistent across the plate. Although TGF-β supplemented condition significantly increased the cell division capability of the PD fibroblasts in the monolayer model, this supplementation was not necessary for our 3D model since TGF-β did not significantly increase the size and circularity of the 3D model. Despite the spheroids' increases in cell density over the culturing period, TGF-β did not affect the cell density in the 3D-cellular structure. Throughout the PD.SPHERE architecture, it also expressed SMA and VIM, the intracellular skeletal markers. Conclusions In conclusion, our model is consistent, reproducible and morphologically relevant to a PD plaque. Our model also demonstrates that we have the potential to produce high throughput treatment screening platforms for PD. Additionally, the PD.SPHERE platform is personalized and customizable to the specific individual; thus, our model indicated the potential to develop this platform for personalized treatment planning. However, signature gene expression and physiological and immunological information (i.e., collagen production, extracellular matrix organization, cellular expression of tight junctions, chemokines, and cytokines) should be further collected to verify the pathophysiological, immunological and treatment response for this model. Disclosure No

Evaluation of Imtasie Health Education Model Based on Kaili Culture to Improve Mother's Behavior in Exclusive Breasting

Evaluating the health education model on exclusive breastfeeding behavior as a basis for the success of the target of achieving exclusive breastfeeding through the implementation of a tested culture-based health education model. This research is quantitative. A total of 84 people who were divided into two groups, namely 42 people in the intervention group and 42 people in the control group were taken purposively according to the inclusion criteria. The intervention group received IMTASIE health education. Evaluate the health education model quantitatively with a quasi-experimental pre-test and post-test with a control group. Data was collected during the pre-test, intervention, and post-test. All data is collected by coaching nurses. The main research variables include myths, culture, knowledge, attitudes, practices and behavior of exclusive breastfeeding. The results of the quantitative study of the developed IMTASIE model had a significant effect on the practice of breastfeeding for 1 month with a p value of 0.001 (<0.05). There is a significant effect with a p value = 0.001 from the interventions carried out by nurses on increasing the practice of exclusive breastfeeding in the first 1 month of life. IMTASIE and its tools can be used in nursing care services individually or in groups and can be carried out in the community or at puskesmas in improving special group nursing care services and improving the quality of life of mothers and babies at an age prone to exclusive breastfeeding due to the influence of myths, culture and knowledge from family.

MP05-16 THE DEVELOPMENT OF A THREE-DIMENSIONAL KIDNEY SPHEROID MODEL TO STUDY CALCIUM-BASED CRYSTAL FORMATION

You have accessJournal of UrologyCME1 Apr 2023MP05-16 THE DEVELOPMENT OF A THREE-DIMENSIONAL KIDNEY SPHEROID MODEL TO STUDY CALCIUM-BASED CRYSTAL FORMATION John Chmiel, Wongsakorn Kiattiburut, Aaron Kwong, Priyanka Prabhu, Hassan Razvi, Leonard Luyt, Jeremy Burton, and Jennifer Bjazevic John ChmielJohn Chmiel More articles by this author , Wongsakorn KiattiburutWongsakorn Kiattiburut More articles by this author , Aaron KwongAaron Kwong More articles by this author , Priyanka PrabhuPriyanka Prabhu More articles by this author , Hassan RazviHassan Razvi More articles by this author , Leonard LuytLeonard Luyt More articles by this author , Jeremy BurtonJeremy Burton More articles by this author , and Jennifer BjazevicJennifer Bjazevic More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003216.16AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: An estimated 10% of North Americans suffer from nephrolithiasis. With rising incidence rates, there is a strong need for models to study the pathophysiology of stone disease. In vitro cell culture models are essential for mechanistic and high-throughput analysis of stone formation and drug treatment; however, there are no suitable models that adequately represent the pathophysiology of stone disease. Advancements in cell culture have given rise to three-dimensional cysts, which are hollow spheroid structures that have apical to basal polarization. These culture systems have similar physiochemical environments to in vivo models and can be cultured for the extended times required for stone formation. Here, we developed a novel 3D kidney cyst model that can generate calcium-based kidney stones. METHODS: Madin-Darby canine kidney (MDCK) cells were cultured in suspension in growth factor-reduced Matrigel to generate hollow spheroid cysts. Crystal formation was induced by culturing cysts in a custom crystal-forming media for two weeks. Immunofluorescent staining was used for characterizing and visualizing the three-dimensional structure. Alendronate-fluorescein, a fluorescently labelled bisphosphonate that specifically binds to calcium-containing crystals, was used along with plain polarized light to visualize the crystal structures. RESULTS: Stains for cellular components and structures demonstrated that the luminal spheroids were successfully formed under the culture conditions. The spheroids also have basal-to-apical polarization suggesting that crystals will form on the inside lumen of the hollow cyst. Birefringent structures resembling calcium oxalate dihydrate and calcium phosphate crystals were observed embedded along the apical face of the spheroid and protruding into the lumen. Alendronate-fluorescein staining confirmed the presence of calcium-containing crystals in the lumen of the cyst. CONCLUSIONS: We have demonstrated that hollow MDCK spheroids cultured with crystal-forming media can generate calcium-containing crystals in the spheroid lumen. This model is the first in vitro tissue culture-based model to reliably develop calcium-based crystals and can be used to investigate their pathogenesis or to perform high-throughput drug screening. Furthermore, it paves the way for personalized medicine by building the foundation for a cell system where renal tissue can be harvested and cultured to identify individual intrinsic risk factors that might contribute to stone development. Source of Funding: Canadian Institutes of Health Research (CIHR) CGS-D, The Northeastern Section of the American Urological Association (NSAUA), Lawson Health Research © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e49 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information John Chmiel More articles by this author Wongsakorn Kiattiburut More articles by this author Aaron Kwong More articles by this author Priyanka Prabhu More articles by this author Hassan Razvi More articles by this author Leonard Luyt More articles by this author Jeremy Burton More articles by this author Jennifer Bjazevic More articles by this author Expand All Advertisement PDF downloadLoading ...

Improving The Organizational Culture of Key Stakeholders Involved in Value Management Process to Achieve Optimum Value in Malaysian Construction

Value Management (VM) is necessary for all construction projects to achieve value for money. There is a need to improve the organizational culture of key stakeholders involved in VM process to achieve optimum value in Malaysian construction projects. This paper is part of a larger study aiming to develop a culture-based organizational model for key stakeholders involved in VM process for achieving optimum value in Malaysian construction projects. This study utilizes a systematic literature review synthesis method to evaluate the trend, identify the gaps and recommend future approach in order to improve the organizational culture of key stakeholders involved in VM process that focuses on achieving optimum value in VM. The results include analyzing current VM status quo in Malaysian construction industry, determining the challenges faced in the VM process of generating innovative ideas, and exploring the project value creation to achieve optimum value. Upon introducing the background problem, the paper deliberates the synthesized literature reviews on VM, key stakeholders, VM process and project performance with VM. A cross-analyses discussion contributes towards formulating the culture-based organizational model for VM practice in Malaysia. The results are expected to improve the integrated project delivery performance of Malaysian construction industry stakeholders by understanding critical VM success factors and weaknesses of VM key stakeholders’ team in generating innovation ideas for optimum project delivery value.

Transformation of Investment Model Implementation in Tourism Industrialization Based on Local Wisdom in Badung Regency

Tourism undergoes a cycle of change that affects the culture-based Balinese tourism model by strengthening local wisdom. Tourism can run optimally if it has guarantees and cultural resilience. In addition to functioning as a tourist attraction, tourism is considered a medium to reap large profits, so tourism activities often shift cultural values and change people's lifestyles. For this reason, to control the influence of globalization on industrialization investment in tourism which has begun to follow the changes in the civilization of the industrial revolution 4.0, Badung Regency has become the rule model for the transformation of the implementation of culture-based tourism investment. This research study is descriptive-analytical, a type of research that does not stop at mere descriptions but also comes to an effort to study the characteristics of solving cases of tourism investment problems using qualitative research methods. The results of this study indicate that the people of Badung Regency's understanding of the importance of local wisdom in applying a culture-based investment model is very high. So investment activities in the tourism sector emphasize aspects of the national economy and preserve local wisdom. In addition, applying a culture-based investment model with strengthening local wisdom requires good synergy between the Government, Indigenous Village communities, tourism businesses, and investors to truly develop the concept of sustainable cultural tourism in Bali.

A Conceptual Model of Culture-Based English Learning Materials in Indonesia

The purpose of this research was to create a culture-based English learning model for use in junior high schools. This study was part of a larger research project that used a research and development (R&D) design. In the first year, the project aims to create a conceptual model of culture-based English learning materials. This study was conducted at Muhammadiyah 1 Junior High School in Ponorogo, East Java province, Indonesia. Muhammadiyah is one of the biggest Islamic non-governmental organizations in Indonesia. We chose this school due to its easy access and low motivation levels of its students in learning English. Sample selection was conducted using a multi-stage sampling technique. The data were collected using questionnaires, document analysis, and interviews, and triangulated using expert judgment. The results of the data analysis led to the production of a conceptual model of English learning material which contains core competencies, basic competencies, themes, or materials of English for junior high school students. The result of the interviews with the students and teachers during need analysis indicated that it is necessary to include local culture in English learning materials to increase students’ engagement and knowledge about their culture. The results of the product try-out showed that students are motivated because they are familiar with the culture-based learning materials. As such, this conceptual model will be the basis to develop similar modules of English learning materials for junior high school students.

Local Culture-Based Music Game Model for Early Childhood Education

Many music experts and educators have researched to see the positive effects of several types of music. Music turns out to be able to affect the intellectual development of children as well as make children wise to socialize. Many facts were revealed from this research. Among them, there is an exciting relationship between music and human intelligence. This study uses a qualitative methodology, with the research object being PIAUD IAIMNU Metro Lampung students. The results of this study indicate that playing music can increase a child's intelligence level because it includes sensitivity to mastery of rhythm, tone patterns, rhythms, tempos, instruments, and musical expressions to be able to sing a song or play it. Learning or playing in early childhood has several advantages, including being able to hone memory because children will recognize and try to memorize the tones of the music.

Synthetic mimetics assigned a major role to IFNAR2 in type I interferon signaling.

Type I interferons (IFNs) are potent inhibitors of viral replication. Here, we reformatted the natural murine and human type I interferon-α/β receptors IFNAR1 and IFNAR2 into fully synthetic biological switches. The transmembrane and intracellular domains of natural IFNAR1 and IFNAR2 were conserved, whereas the extracellular domains were exchanged by nanobodies directed against the fluorescent proteins Green fluorescent protein (GFP) and mCherry. Using this approach, multimeric single-binding GFP-mCherry ligands induced synthetic IFNAR1/IFNAR2 receptor complexes and initiated STAT1/2 mediated signal transduction via Jak1 and Tyk2. Homodimeric GFP and mCherry ligands showed that IFNAR2 but not IFNAR1 homodimers were sufficient to induce STAT1/2 signaling. Transcriptome analysis revealed that synthetic murine type I IFN signaling was highly comparable to IFNα4 signaling. Moreover, replication of vesicular stomatitis virus (VSV) in a cell culture-based viral infection model using MC57 cells was significantly inhibited after stimulation with synthetic ligands. Using intracellular deletion variants and point mutations, Y510 and Y335 in murine IFNAR2 were verified as unique phosphorylation sites for STAT1/2 activation, whereas the other tyrosine residues in IFNAR1 and IFNAR2 were not involved in STAT1/2 phosphorylation. Comparative analysis of synthetic human IFNARs supports this finding. In summary, our data showed that synthetic type I IFN signal transduction is originating from IFNAR2 rather than IFNAR1.

Ceiling culture of human mature white adipocytes with a browning agent: A novel approach to induce transdifferentiation into beige adipocytes.

Excess and dysfunctional adipose tissue plays an important role in metabolic diseases, including obesity, atherosclerosis and type 2 diabetes mellitus. In mammals, adipose tissue is categorized into two types: white and brown. Adult brown tissue is mainly composed of beige adipocytes, which dispose of stored energy as heat and have become increasingly popular as a therapeutic target for obesity. However, there is still a paucity of cell models that allow transdifferentiation of mature white adipocytes into beige adipocytes, as seen in vivo. Here, we describe a novel, ceiling culture-based model of human mature white adipocytes, which transdifferentiate into beige adipocytes under the mechanical force and hypoxia of ceiling culture. We also show that the use of rosiglitazone and rapamycin can modulate transdifferentiation, up and down regulating expression of beige adipocyte-specific genes, respectively. Rosiglitazone additionally facilitated the upregulation of fatty acid lipolysis and oxidation genes. Finally, these beige adipocytes derived from dedifferentiated adipocytes exhibited a progenitor-specific phenotype, with higher expression of mature adipocyte-specific genes than adipocyte-derived stem cells. Overall, we report a novel approach to conveniently cultivate beige adipocytes from white adipocytes in vitro, suitable for mechanistic studies of adipose biology and development of cell and drug therapies in the future.