Orchid Cultivation Research Articles

Community Economic Empowerment in the Ngoro Village PKK group through Orchid Cultivation

Orchids are a type of diverse and beautiful flower plant found throughout the world, including Indonesia. Orchids naturally thrive in forest environments or areas with abundant moisture and organic nutrients. Considering the decreasing area of forest, it is important to take immediate action to preserve and protect various orchid species that are threatened with extinction. The Ngoro Village PKK group together with Damar Farm held orchid planting training which highlighted the potential for cultivating quality orchid plants. The aim of this effort is to provide education to the local community and supervise orchid cultivation as a means of earning a living for the family. The approach taken is through practice and question and answer sessions. Participants included sub-district heads and women from the PKK group in Ngoro Village. This material provides an overview of orchids, including the characteristics, potential, and techniques for propagating and cultivating these plants. The results of these efforts resulted in a significant increase in participants' understanding of orchid plants. There are two types of orchids that are commonly used for training: pigeon orchids (Dendrobium crumenatum) and ground orchids (Spathoglottis plicata). This orchid is famous for its ease of reproduction by separating young plants. Coconut fiber is an orchid planting medium that is often found in this area.

PEMASARAN DAN BUDI DAYA BUNGA ANGGREK DI PAMULANG TANGERANG SELATAN

The orchid cultivation and marketing industry in South Tangerang has shown significant development within the horticultural sector. This study explores the general conditions of orchid cultivation, including environmental factors conducive to plant growth and common cultivation techniques. Additionally, the study delves into aspects of orchid marketing, covering strategies employed in marketing local orchid products and addressing challenges in both local and export markets. In the context of technology, the research highlights the role of modern technology in orchid cultivation development, from growth monitoring to harvest processing systems. Key constraints in the industry are also discussed, alongside case studies of successful farmers or companies navigating challenges and leveraging opportunities in the orchid cultivation and marketing industry. The findings aim to provide valuable insights for further development in the orchid industry in South Tangerang, serving as a foundation for sustainable policies and best practices to advance the sector

Pesticide contamination pattern of surface water in an urban–agricultural mediterranean watershed (Wadi Guenniche, Bizerte Lagoon, Northern Tunisia)

Two years of monthly sampling and hydrological monitoring were performed at the outlet of a Mediterranean watershed in northern Tunisia to determine the contents of 469 pesticide active ingredients and metabolites in water and evaluate their behavior. Wadi Guenniche is a tributary of the Bizerte coastal lagoon, with a watershed area of 86 km2, which exhibits pluvial cereal, legume, and orchid cultivation and irrigated market gardening. Twenty-nine pesticide active ingredients and 2 metabolites were detected in water. Twenty-four pesticide active ingredients were authorized for use in Tunisia. Among them, 14 had never been mentioned in previous farmer surveys. Five herbicides and their metabolites were the most frequently detected: aminomethylphosphonic acid (AMPA) (100%), glyphosate (94%), simazine (94%), 2,4-D (70%), and deisopropylatrazine (DIA) (47%). The detection frequency and concentration range suggested that the phytosanitary pressure and resulting water contamination are close to those on the northern Mediterranean shore. These results, in addition to characterizing the pollution state, emphasized the need for additional studies on the use and fate of pesticides on the southern shore of the Mediterranean Sea, particularly in Tunisia.

IoT revolutionizes orchid cultivation in Indonesia with precision and efficiency

The Orchidaceae family is highly valued in ornamental, perfume, medical, and cosmetic industries, but cultivation often fails due to human error. This research aims to design an IoT-integrated automation system to monitor and control key parameters such as air temperature, humidity, and moss moisture for orchid cultivation. Using the research and development method, we observed plant needs, reviewed relevant literature, designed an effective system, and tested sensor accuracy and system reliability. Results showed the DHT-22 sensor had a minimal error in temperature (0.9%) and humidity (2.1%) readings, and the soil moisture sensor had a 3.65% error. The system maintained optimal conditions, and the Blynk app effectively displayed data. This innovation reduces human error and enhances orchid cultivation efficiency, with potential applications in other sensitive plant systems. Highlight: Error Reduction: IoT system minimizes human error in orchid cultivation. Sensor Accuracy: DHT-22 sensors show minimal temperature (0.9%) and humidity (2.1%) errors. Optimal Conditions: Maintains air humidity 40%-80%RH and moss moisture 50%-80%RH. Keyword: Orchidaceae, IoT automation, sensor accuracy, environmental control, orchid cultivation

The Impact of Music on Orchid plants Growth in Polyhouse Environments

Nature and music, two seemingly disparate elements of human experience, are deeply interconnected in ways that resonate across cultures and time. Both evoke emotions, stir the soul, and inspire creativity, offering profound insights into the human condition and our relationship with the world around us. In essence, nature and music are two expressions of the same universal impulse—the longing to connect with something greater than ourselves, to find beauty and meaning in the world around us. Whether we're listening to the songs of birds at dawn or the symphonies of Beethoven, we are reminded of our place in the vast tapestry of creation, where every note, every sound, is a testament to the wonder and mystery of existence. By carefully considering the environmental factors and designing experiments that account for them, researchers can gain a better understanding of how music influences plant growth and optimize its use in agricultural and horticultural practices. This research paper investigates the potential effects of music on orchid growth within polyhouse environments. Orchids are renowned for their delicate beauty and are cultivated worldwide, often in controlled environments like polyhouses. While studies have explored the influence of various environmental factors on orchid growth, the impact of music remains relatively unexplored. This study aims to fill this gap by examining how exposure to music may affect the growth, development, and overall health of orchids in polyhouse conditions. The research employs a combination of experimental methods, including controlled trials, measurements of plant growth parameters, and analysis of physiological indicators. Additionally, the study considers factors such as the type of music, frequency, volume, and duration of exposure. The findings of this research contribute to our understanding of the complex relationship between plants and their acoustic environment, with potential implications for optimizing orchid cultivation practices in polyhouse settings.

Maximizing Zygopetalum maculatum orchid yield through calcium nutrition: Unveiling calcium content and dynamics across different plant parts at different growth stages of the orchid

This study explores the pivotal yet often overlooked role of calcium (Ca) in Zygopetalum maculatum orchid cultivation. Six different treatments, featuring graded doses of Ca (ranging from 0 to 100 mg Ca L−1 nutrient solution), were used to assess the impact of Ca on growth, development, and physiological responses of the orchids. Notably, the Ca untreated control treatment (Ca0) exhibited a rapid decline in potting media pH (became acidic in reaction with time), whereas under Ca100 treatment potting media pH declined slowly and remained near neutral. Dehydrogenase activity in the potting media significantly increased under Ca100 treatment. Significant improvements in vegetative growth parameters, like, bulb length, bulb width, and leaf characteristics, were evident under Ca100 treatment compared to the Ca0 treatment. The economic yield, measured by the number of flower spikes and various floral attributes (i.e. number of florets per spike, spike length, florets size), displayed a remarkable enhancement under Ca100 treatment. The Ca100 treatment produced 100 % more number of flower spikes as compared to Ca0 treatment. The number of new shoot initiation, and overall flower biomass also substantially increased under Ca100 treatment. Ca content analysis in different plant parts revealed presence of elevated Ca concentrations in leaves, back bulbs, new bulbs, and roots under Ca100 treatment. The Ca content in back bulbs and roots decreased after flowering phase of the orchid, but the Ca levels in leaves and new bulbs remained statistically similar during both pre and post-flowering phases.These findings underscore the positive influence of Ca application on Zygopetalum maculatum orchids for enhancing its vegetative growth and economic yield. The study contributes valuable insights into Ca dynamics in different plant parts of the orchid, and recommends a 100 mg L−1 Ca application at 7-day intervals for improving Zygopetalum maculatum cultivation practices.

The Effect Of Shoot Cuttings And Vermiwash Concentration On Orchid Growth (Onchidium sphacelatum Lindl.) Aeroponically

The Oncidium sphacelatum orchid, commonly propagated vegetatively through pseudobulb separation, presents opportunities for further research into propagation methods and organic fertilizer application. This study, conducted in Batu City, aimed to explore the interaction between vermiwash concentration and shoot number on aeroponically cultivated Orchid sphacelatum Lind cuttings, as well as individual treatment effects. Employing a factorial RKLT design with shoot number (one or two) and vermiwash concentration (100, 200, or 300 ml/L) as factors, the study evaluated plant height, leaf count, stem length, shoot length, root count, and root length as variables. Data analysis involved ANOVA and BNJ tests. Results revealed that cuttings with two shoots and a 200 ml/L vermiwash concentration promoted optimal leaf growth. The number of shoots influenced shoot length, with two shoots demonstrating superior growth compared to single shoot cuttings. Interestingly, vermiwash concentration did not significantly impact shoot growth parameters, indicating that its effects may be limited in this context. This study sheds light on the potential benefits of using vermiwash in orchid cultivation, particularly in enhancing leaf growth when combined with multiple shoot cuttings. However, further investigation is needed to understand the nuanced effects of vermiwash on other growth aspects and its interaction with different propagation methods.Overall, the findings contribute to the optimization of orchid propagation techniques, providing valuable insights for orchid growers seeking to enhance plant growth and productivity sustainably. Further research could delve deeper into the mechanisms underlying the observed effects and explore additional variables to refine cultivation practices for Oncidium sphacelatum orchids.

Pemberdayaan Santri Melalui Pendampingan Budidaya Anggrek Di Pondok Pesantren Nurul Qur’an, Desa Kebulusan, Kabupaten Kebumen

The Nurul Qur'an Islamic Boarding School, which is located 5 KM from the city center of Kebumen Regency, has great economic development potential because of its strategic location. In order to increase the independence of students, it is necessary to make efforts to provide students with skills. One of the efforts made is to provide orchid cultivation training to students. The location of Islamic boarding schools close to the city center provides opportunities for students to develop ornamental orchid cultivation. The high interest of city residents in ornamental plants is the main reason for choosing orchids as the focus of development. Orchids are considered to have high aesthetic value and stable sales value, making them the right choice to develop. This service aims to provide motivation, knowledge and skills to students regarding orchid cultivation, with the hope of creating student independence. The method applied involves providing materials related to repotting, plant maintenance including fertilizing, watering, and inducing orchid flowering. Discussion sessions, making demonstration plots, and assistance with orchid cultivation practices are also an integral part of the implementation method. The results of this service show an increase in the students' competence in orchid maintenance and they are able to form an orchid agribusiness forum which is managed independently by the students. Thus, this service not only provides benefits in terms of improving practical skills, but also opens up new economic opportunities for students at the Nurul Qur'an Islamic Boarding School.

Effectiveness of Bottle Size Variation on the Growth of Dendrobium Striaenopsis Orchid Plantlets

The production of Dendrobium striaenopsis orchid cultivation in Indonesia has many obstacles including limited seed provision, low seed quality and cultivation techniques that have not been done well, while orchid enthusiasts always increase every year. Propagation of orchid plants by conventional means requires a long time to obtain a large number of plant saplings, therefore alternative cultivation using tissue culture techniques is needed. In vitro tissue culture techniques are widely used as a fast and efficient way to produce D. striaenopsis orchid plants, but not infrequently there are factors that affect the production of orchids, one of which is bottle size. The purpose of this study is to determine the effect of the effectiveness of bottle size variations on the growth of D. striaenopsis plantlets after comparing the difference in bottle volume and knowing the optimum size of bottles used for D. striaenopsis plantlet growth. The study was conducted from May to August 2023 in the Laboratory CV. Candi Orchid, Semarang City. The method used was the observation and experimental method after which it was continued with the One Way Anova test and further test T. In this study using Vacin and Went medium with a size of 60 ml by providing treatment variations in bottle sizes of 250 ml, 500 ml and 750 ml with 4 repeats. The result of this study is that a 750 ml bottle used in the tissue culture technique of D. striaenopsis Orchid is effective in use even though the growth of plantlets does not occur a significant difference, this is due to environmental factors, namely light intensity and duration of irradiation

Identification of Viruses Infecting Phalaenopsis Orchids Using Nanopore Sequencing and Development of an RT-RPA-CRISPR/Cas12a for Rapid Visual Detection of Nerine Latent Virus.

Phalaenopsis orchids are one of the most popular ornamental plants. More than thirty orchid viruses have been reported, and virus-infected Phalaenopsis orchids significantly lose their commercial value. Therefore, the development of improved viral disease detection methods could be useful for quality control in orchid cultivation. In this study, we first utilized the MinION, a portable sequencing device based on Oxford Nanopore Technologies (ONT) to rapidly detect plant viruses in Phalaenopsis orchids. Nanopore sequencing revealed the presence of three plant viruses in Phalaenopsis orchids: odontoglossum ringspot virus, cymbidium mosaic virus, and nerine latent virus (NeLV). Furthermore, for the first time, we detected NeLV infection in Phalaenopsis orchids using nanopore sequencing and developed the reverse transcription-recombinase polymerase amplification (RT-RPA)-CRISPR/Cas12a method for rapid, instrument-flexible, and accurate diagnosis. The developed RT-RPA-CRISPR/Cas12a technique can confirm NeLV infection in less than 20 min and exhibits no cross-reactivity with other viruses. To determine the sensitivity of RT-RPA-CRISPR/Cas12a for NeLV, we compared it with RT-PCR using serially diluted transcripts and found a detection limit of 10 zg/μL, which is approximately 1000-fold more sensitive. Taken together, the ONT platform offers an efficient strategy for monitoring plant viral pathogens, and the RT-RPA-CRISPR/Cas12a method has great potential as a useful tool for the rapid and sensitive diagnosis of NeLV.

Advances in Orchid Biology: Biotechnological Achievements, Translational Success, and Commercial Outcomes

Orchids constitute the largest and most diverse group of flowering plants and are classified in the family Orchidaceae. Exhibiting significance as the most exotic and ubiquitous flowering plant, the cultivation of orchids on a commercial level is gaining momentum worldwide. In addition to its ornamental and aesthetic value, the orchid industry has successfully generated employment for people in developing countries. Recent advances in biotechnological interventions in orchids have substantially contributed to the development of exotic varieties with novel traits, not to forget the inputs of traditional plant breeding methods and tissue culture approaches. In addition, the scientific developments in orchid biology have remarkably bridged the knowledge gaps in areas of orchid classification, phytochemistry, and cultivation strategies. This has facilitated the commercialization of novel varieties, opening new avenues in the orchid industry, and their global marketing as cut flowers and artificially propagated plants. Orchids constitute the first floriculture crops that revolutionized the orchid industry; however, they also hold several challenges in the natural propagation and conservation of several species that are on the verge of extinction. International organizations like CITES have come forward to address challenges associated with illegal global trade and indiscriminate use of orchid varieties, aiming for conservation and legal commercial goals. This thematic review is one-of-a-kind in providing comprehensive insights into the emerging momentum of orchid biology and how its globalization projects to considerably impact the orchid industry in the coming times. However, it is imperative to understand the challenges in the cultivation and conservation of orchid varieties and ensure legislative guidelines both on domestic and global levels to ensure a multipronged approach to the conservation and commercialization of orchids.

Effect of Benzyl Amino Purine (BAP) on the Leaf Growth of Vanda limbata Blume Orchid In Vivo

<p><em>Vanda limbata</em> is a natural orchid with slow growth and is often taken directly from the forest in large quantities, threatening its sustainability. Ex-situ preservation efforts are needed for this orchid, one of which is through cultivation with the addition of Benzyl Amino Purin (BAP), known to increase shoot growth. This research aims to analyze the effect of BAP and determine its optimal concentration on the growth of <em>V. limbata</em> orchids. This research used a single-factor completely randomized design in BAP concentrations of 0, 25 and 50 mg l<sup>-1</sup> with 5 replications. The method used was spraying BAP solution once a week on all parts of adult <em>V. limbata</em> orchids aged ±2 years. The research was conducted for 3 months in the experimental garden with parameters observed in the form of the number of new leaves, elongation of old leaves, the length of new leaves, increase in the width of old leaves, the width of new leaves, stomatal density and the number of roots. The results showed that the most effective BAP concentration was 50 mg l<sup>-1</sup> for the number of new leaves which increased by 200%, the length of old leaves which increased by 168.66% and the length of new leaves which increased by 800%, but resulted in less stomatal density than the control. BAP can increase the growth of <em>V. limbata</em> orchid leaves. This research can be a recommendation for ex-situ conservation efforts that have the potential to be developed as a means of orchid cultivation.</p>

Improved use of mycorrhizal fungi for upscaling of orchid cultivation and population restoration of endangered orchids in China

Improved use of mycorrhizal fungi for upscaling of orchid cultivation and population restoration of endangered orchids in China

Managemen Produksi Dan Distribusi Tanaman Anggrek di DD Orchid Nursery

Orchid plants are one of the ornamental plants that are widely known to the public in Indonesia and abroad. This type of plant was originally an epirite plant attached to other plants, but it produces very beautiful flowers. since the last decade, plant business activities have played a significant role in the center of economic growth. Batu City is a center for orchid production and marketing in East Java which is a priority for orchid plant development because orchid cultivation has added value when compared to ornamental plant businesses in general. The service method carried out is in the form of orchid cultivation training which includes: Making orchid plant packaging, orchid marketing strategy, customer segmentation, and evaluation method. Marketing agency involved in the distribution of official orchid plants that serve consumers such as hobbyists, collectors, and retailers around the Batu area. The quality of orchids can be seen from the stem of the orchid plant, the height of the orchid plant and the type of orchid plant. The selling price that occurs is basically through bargaining. determining the price of orchids in Batu is close to a free market where every farmer is free to determine the price as long as it does not deviate from the company. The payment system can be done in cash or transfer. Marketing of orchids involves several marketing institutions, namely retailers and collectors. Management of production and distribution of export quality orchids can be achieved with the support and methods that must be carried out such as good packaging of orchids, sophisticated marketing strategies, targeted customer segmentation and collaboration between institutions

Optimizing Accounting Practices for Financial Management in Orchid Cultivation: A Comprehensive Approach for Farmers in Dadaprejo Junrejo Village, Batu City

Founded in 2007, DD Orchid in Dadaprejo, Junrejo District, Batu City, is a notable player in orchid cultivation, offering a diverse range of orchids at various price points. Despite its success, the organization faces challenges such as irregular bookkeeping, disorganized stock management, and a lack of basic bookkeeping training for collaborating farmers. This empirical research investigates the impact of proposed solutions on enhancing operational efficiency. The study, spanning a 2-hectare property with advanced watering systems and involving 80-100 plasma-assisted farmers, addresses these challenges through strategic interventions. Firstly, it explores the impact of elevating human resource quality within DD Orchid. Secondly, the implementation of disciplined administrative and financial practices, including regular financial statements, is examined for its effect on overall management. Thirdly, the study evaluates the impact of optimizing orchid code management. Lastly, the research investigates the effectiveness of providing training to plasma-assisted farmers on creating simple financial reports. The anticipated results aim to provide empirical evidence on the effectiveness of these solutions in streamlining administrative, financial, bookkeeping, and human resource management within DD Orchid and among its collaborating farmers.

Analisis Strategi Pemasaran Tanaman Hias Anggrek di Tangerang Selatan: Peluang dan Tantangan bagi Produsen Lokal

This study aims to analyze the marketing strategy of orchid ornamental plants in South Tangerang, especially in the context of opportunities and challenges faced by local producers. Conducted in December 2023, this study took objects from 10 housewives cultivating orchid ornamental plants in South Tangerang City. The method used is descriptive qualitative, by conducting in-depth interviews and observations of local producers to understand the marketing strategies they use, as well as identifying opportunities and challenges faced in marketing their products. The results showed that local producers used a variety of marketing strategies, including local exhibitions, online sales, and cooperation with local florists. However, there are several challenges faced, including limited market access, price competition with imported products, and lack of understanding of digital marketing. In-depth discussions are conducted to evaluate effective marketing strategies and overcome the challenges faced. The implications of this research highlight the importance of developing innovative and adaptive marketing strategies for local producers in the face of increasingly fierce market competition. Recommendations include improving market access through cooperation between local producers, training on digital marketing, and improving local product branding to increase competitiveness in local and international markets. Thus, this research contributes to strengthening the local orchid cultivation sector in South Tangerang.

IOT Implementation Energy consumption for Indoor Orchid Cultivation based on WeMos

Orchids are epiphytic plants with high popularity. Orchid is a plant that can process its own nutrients with the help of other media. Parameters that need to be monitored include air temperature, air humidity, and soil moisture. These parameters greatly affect plant growth. Plants can grow well if humidity, temperature, and light intensity are met properly. Good orchid humidity ranges from 50 – 80%. Humidity should not be too high at night and not too low during the day. In addition to humidity, the temperature where you plant orchids must also be maintained. The ideal temperature for orchids is daytime temperatures between 27-30 degrees Celsius and night temperatures between 21-24 degrees Celsius. Based on these problems, a Design and Development Tool for Monitoring Soil Temperature and Moisture on the Growth of Indoor Orchid Plants is Based on WeMos with Indoor Energy. In this design, the results of sensor readings will be displayed in the form of data which will be accessed via the web server on WeMos. To read the temperature, air humidity, and soil moisture, several sensors are used, namely: DHT 22, soil moisture sensor, and a relay connected to a mini water pump using an indoor cultivation method where the energy source uses UV waves by LED light. From the results of the implementation and testing, the growth period will be obtained more faster than the normal cultivation. hopefully, this research will help people who care for orchid plants to be able to monitor their orchid plants efficiently in indoor conditions so that these orchid plants can always be in the best conditions of temperature, light, and soil moisture.

Growth-promoting microorganisms in the development of orchid seedlings of Phalaenopsis, Cymbidium, and Dendrobium genera

The demand for sustainable agricultural production systems is increasing, and using growth-promoting microorganisms in plants has stood out because it decreases or even replaces chemical fertilizer use, reducing production costs. This study aimed to evaluate the response of some microorganisms applied to the seedlings of primary orchids cultivated in Brazil (Phalaenopsis sp. 'Taisuco Swan', Cymbidium atropurpureum, and Dendrobium secundum). The experimental design was completely randomized. There were seven treatments (absence of microorganisms – control, Trichoderma sp. in sodium alginate, Trichoderma sp. in clay, Trichoderma sp. in sodium alginate and clay, Trichoderma sp. in a liquid medium, Azospirillum brasilense + Bacillus subtilis in a liquid medium, and Bacillus pumilus in a liquid medium), four replications, and three plants per plot. The seedlings were grown in a greenhouse and evaluated 190 days after microorganism inoculation. The evaluation of morpho-physiological characteristics differed according to the particularities of each genus. The Bacillus pumilus and Azospirillum brasilense + Bacillus subtilis rhizobacteria in a liquid medium for Phalaenopsis sp. 'Taisuco Swan' and the Trichoderma sp. fungus in a liquid medium for Cymbidium atropurpureum increased seedling growth and development. Azospirillum brasilense + Bacillus subtilis in a liquid medium for the Dendrobium secundum orchid promoted more root biomass. Using beneficial microorganisms in orchid cultivation is promising, and seedling growth and development depend on their inoculation and the morpho-physiological characteristics of each plant.

MANAJEMEN RANTAI NILAI KOMODITAS ANGGREK DI PT ANUGERAH ANGGREK NUSANTARA KABUPATEN KEDIRI

The value chain for the development of orchid commodities at PT Anugerah Anggrek Nusantara has several obstacles, namely the agro-climate that does not support orchid cultivation, low technological innovation, and limited stock availability so that consumer demand cannot be fulfilled optimally. This research was conducted from August to December 2022 at PT Anugerah Anggrek Nusantara Kediri Regency, East Java. Sources of data used, namely primary data through observation, interviews, and documentation, while secondary data through literature studies. The results of the study show that value chain management at PT Anugerah Anggrek Nusantara is carried out with several activities including, procurement of raw material inputs, production processes, and marketing. Procurement of production inputs depends on the activities of the production stages obtained from offline and online stores. Production activities at PT Anugerah Anggrek Nusantara consist of pollination, tissue culture, acclimatization, repotting, maintenance, harvesting and marketing. Marketing activities at this company apply the marketing strategy of the 7P marketing mix, namely product, price, promotion, place, people, process, and physical evidence.

Suggesting a prospect for melatonin-mediated orchid flowering under extreme conditions

Abiotic stress is a major constraint in the successful growth and development of ornamental plants under severe conditions. Since antiquity, melatonin homeostasis has been an effective contrivance in the strategies of abiotic stress tolerance in plants, although it is completely neglected in orchids, the champagne of flowers. Orchids with exceptionally long vegetative phase face a number of stresses and, therefore, stress alleviation has been a hot topic in orchid cultivation. The in-vitro flowering is a useful method for early identification of hybrids in crop improvement programs. We successfully achieved in-vitro leafless flowering in three Cymbidium orchids (C. sinense, C. goeringii and C. ensifolium), which are elite flowers in terms of versatile flower shapes, esthetic preference and price. Transcriptomic analysis was performed to compare the leafless flowers with leaves to identify genes related to tryptophan pathway and melatonin biosynthesis. More than 50 differentially expressed genes (DEGs) were identified in each species with contrasting expressions in the leafless flowers and leaves. The important melatonin pathway genes included serotonin N-acetyltransferase (SNAT), caffeic acid 3-O-methyltransferase (CAMT) and tryptophan decarboxylase (TDC). Moreover, clustering analysis pointed out the significant abiotic stress tolerance-related genes. This opening report, thus, provides a robust cradle to idealize the melatonin as an effective abiotic stress regulator to aid the long journey of vegetative growth and then flowering in precious orchids.