Cultivation Of Edible Mushrooms Research Articles

Loop-mediated isothermal amplification (LAMP) assay coupled with gold nanoparticles for colorimetric detection of Trichoderma spp. in Agaricus bisporus cultivation substrates

One of the significant challenges in organic cultivation of edible mushrooms is the control of invasive Trichoderma species that can hinder the mushroom production and lead to economic losses. Here, we present a novel loop-mediated isothermal amplification (LAMP) assay coupled with gold nanoparticles (AuNPs) for rapid colorimetric detection of Trichoderma spp. The specificity of LAMP primers designed on the tef1 gene was validated in silico and through gel-electrophoresis on Trichoderma harzianum and non-target soil-borne fungal and bacterial strains. LAMP amplification of genomic DNA templates was performed at 65 °C for only 30 min. The results were rapidly visualized in a microplate format within less than 5 min. The assay is based on salt-induced aggregation of AuNPs that is being prevented by the amplicons produced in case of positive LAMP reaction. As the solution color changes from red to violet upon nanoparticle aggregation can be observed with the naked eye, the developed LAMP-AuNPs assay can be easily operated to provide a simple initial screening for the rapid detection of Trichoderma in button mushroom cultivation substrate.

Identification and fungicides sensitivity evaluation of the causal agent of cobweb disease on Lyophyllum decastes in China

BackgroundCobweb disease is a fungal disease that commonly affects the cultivation and production of edible mushrooms, leading to serious yield and economic losses. It is considered a major fungal disease in the realm of edible mushrooms. The symptoms of cobweb disease were found during the cultivation of Lyophyllum decastes. This study aimed to identify the causative pathogen of cobweb disease and evaluate effective fungicides, providing valuable insights for field control and management of L. decastes cobweb disease.ResultsThe causal agent of cobweb disease was isolated from samples infected and identified as Cladobotryum mycophilum based on morphological and cultural characteristics, as well as multi-locus phylogeny analysis (ITS, RPB1, RPB2, and TEF1-α). Pathogenicity tests further confirmed C. mycophilum as the responsible pathogen for this condition. Among the selected fungicides, Prochloraz-manganese chloride complex, Trifloxystrobin, tebuconazole, and Difenoconazole exhibited significant inhibitory effects on the pathogen’s mycelium, with EC50 values of 0.076 µg/mL, 0.173 µg/mL, and 0.364 µg/mL, respectively. These fungicides can serve as references for future field control of cobweb disease in L. decastes.ConclusionThis study is the first report of C. mycophilum as the causing agent of cobweb disease in L. decastes in China. Notably, Prochloraz-manganese chloride complex demonstrated the strongest inhibitory efficacy against C. mycophilum.

Effect of Different LED Light Wavelengths on Production and Quality of Pleurotus ostreatus Grown on Different Commercial Substrates

Artificial lighting, primarily employed in crop production, can also be applied to the cultivation of edible mushrooms to enhance productivity and quality. While UV radiation has predominantly been investigated in post-harvest treatments for edible mushrooms, the utilization of different light wavelengths during the cultivation phase remains largely unexplored for many mushroom species. This study aimed to assess the impact of three different light wavelengths 450 nm (B), 610 nm (R), and a combination of these two wavelengths (R + B) on the productive characteristics and quality of Pleurotus ostreatus, cultivated using three straw-based commercial substrates. It was observed that, except for yield, artificial light influenced mushroom growth. Specifically, the application of R light appeared to promote mycelium growth, whereas B light contributed to increase the diameter of fruiting bodies. Additionally, the concentration of vitamin D2 was higher under both B and R+B light treatments. Interestingly, the light treatments did not affect yield but impacted diameter and various chemical attributes such as EC, total soluble solids, and titratable acidity. In conclusion, exposure to different lighting affected Pleurotus ostreatus physiology and nutritional content.

Isolation and Identification of dsRNA Mycovirus from Cultivated Mushroom Lentinula edodes

Viruses that infect the edible mushroom Lentinula edodes have been documented to exhibit either disease expression or remain asymptomatic. Nonetheless, our knowledge regarding viruses infecting L. edodes, as well as their diversity, remains relatively limited. This research aimed to isolate double-stranded RNA (dsRNA) molecules from 25 economically significant mushrooms using dsRNA extraction and gel electrophoresis techniques. The results revealed the presence of MSU16 dsRNA segments with a size of approximately 10,000 bp in a Lentinula sp. mushroom sample. This substantial dsRNA suggested the possible identification of the virus within the group of Lentinula edodes mycoviruses. Characterization of the MSU16 dsRNA molecule using a 2-step RT-PCR technique, DNA sequencing, and phylogenetic tree analysis based on partial RdRp nucleotide and amino acid sequences indicated a close relationship to other mushroom viruses found in Lentinula sp., with similarity ranging from 39.4 to 64.6 %. Molecular classification of the ITS gene sequence of the dsRNA-detected MSU16 showed a 92 % identity with L. edodes. However, the mushroom MSU16 could not be classified based on the limited LSU gene sequence available in GenBank. This study suggests that the MSU16 dsRNA molecule represents a Lentinula edodes dsRNA mycovirus isolate from Thailand. Our findings contribute valuable information about mycoviral presence in cultivated edible mushrooms. HIGHLIGHTS An approximately 10,000 bp dsRNA segment was successfully isolated from the Lentinula edodes mushroom isolate MSU16. This dsRNA was characterized through a 2-step RT-PCR, DNA sequencing, and phylogenetic analysis, focusing on partial RdRp nucleotide and amino acid sequences. The analysis indicated a close relationship between the isolated dsRNA and other viruses known to infect L. edodes mushrooms. Furthermore, the ITS gene sequence of MSU16 exhibited a high similarity to the corresponding ITS sequences in L. edodes. GRAPHICAL ABSTRACT

Processes For The Transformation Of Fruit And Vegetable Residues For The Production Of Edible Mushrooms

The cultivation of edible mushrooms from fruit and vegetable residues presents itself as a promising solution for the sustainable utilization of these wastes and the production of nutritious food. Fruit and vegetable residues include crop residues, leaves, branches and unsold products, which contain a wide range of nutrients and bioactive compounds. These are used as substrate for the cultivation of edible mushrooms. Edible mushrooms, such as mushrooms, shiitake and pleurotus, are appreciated worldwide for their taste, texture and nutritional properties. They contain protein, fiber, vitamins, minerals and bioactive compounds with antioxidant, anti-cancer and anti-diabetic properties. In addition, the global market for edible mushrooms is experiencing significant growth due to their wide range of applications in the food, pharmaceutical and cosmetic industries. The method of cultivating edible mushrooms from fruit and vegetable waste involves the selection of suitable waste, its preparation by sterilization, addition of nutritional enrichment agents and inoculation of the desired mushroom. After a period of incubation and proper care, the mushrooms can be harvested and used in various food preparations. The cultivation of edible mushrooms by solid fermentation has great advantages, as it is the most efficient way of converting vegetable waste into high-value human food.

Lignocellulosic supplementation of the culture medium for mycelial development and production of phenoloxidase enzymes from Amazonian strains of gasteroid fungi (Basidiomycota)

Strains of gasteroid fungi are capable of oxidizing phenolic components and producing enzymes of great commercial value. However, the difficulty in producing biomass and, consequently, the low levels of expression of enzymes produced by these fungi hinder the advancement of biotechnological studies. A possible alternative may be supplementing the culture medium with lignocellulosic substrates, as observed in the cultivation of edible mushrooms, for example. Thus, the objective of this study was to analyze the effect of supplementing a potato-based culture medium with different concentrations of wheat and rice bran on mycelial development and the production of phenoloxidase enzymes of Amazonian strains of Cyathus and Geastrum. The basidiomas were collected in the Amazon of Pará, and the strains were obtained from mycelial isolation on Potato Dextrose Agar (PDA) medium. The strains were inoculated in semi-solid and liquid potato-based medium containing 10% and 20% rice bran and 10% and 20% wheat bran. For a period of 20 days, mycelial growth was observed in solid form, and for 40 days, the development of the strains in liquid medium was monitored, all incubated at 25°C in the dark. Growth and production of fresh and dry biomass were measured, as well as the production of phenoloxidase, arising from growth in liquid medium. Supplementation using 10% wheat bran was the most promising for the radial growth of four of the six species tested (C. limbatus, G. hirsutum, G. echinulatum, and G. schweinitzii), in addition to being the best treatment for the production of biomass and phenoloxidase enzymes for all species. Considering that wheat bran is easily accessible, this study stimulates basic science, especially in the Amazon, where Funga is still underestimated in terms of diversity and biotechnological potential.

The Metacaspase Gene PoMCA1 Enhances the Mycelial Heat Stress Tolerance and Regulates the Fruiting Body Development of Pleurotus ostreatus

Pleurotus ostreatus is one of the most cultivated edible mushrooms worldwide, of which the fruiting body development is a highly complex process involving the precise genetic regulatory network and suitable environmental factors. Metacaspases play important roles in developmental processes and programmed cell death (PCD) induced by some environmental stress in many organisms. In this study, a type I metacaspase, PoMCA1, was identified via the analysis of the enzyme domain and alignment with homologous metacaspases. PoMCA1 overexpression and RNAi mutants were generated via Agrobacterium tumefaciens-mediated transformation (ATMT) into the P. ostreatus mycelium. The roles of the PoMCA1 gene in heat stress and fruiting body development were examined. The results show that both of the overexpression transformants were more tolerant to heat stress than the wild-type strain, while the opposite phenomenons were found for the two RNAi strains. Compared with the wild-type strain, the overexpression strain OE-7 had faster formation of the fruiting body, while the two RNAi strains produced significantly more primordia and young fruiting bodies, and presented morphological deformities and slower fruiting body development. All of the results suggest that the PoMCA1 gene is involved in the positive regulation of heat stress tolerance and fruiting body development in P. ostreatus.

Label-free comparative proteomic analysis of Pleurotus eryngii grown on sawdust, bagasse, and peanut shell substrates

The white rot fungi Pleurotus eryngii are environmental microorganisms that can effectively break down lignocellulosic biomass. However, understanding of the mechanisms by which P. eryngii is effective in degrading lignocellulose is still limited. This work aimed to examine the extracellular secretory proteins implicated in the breakdown of lignocellulose in P. eryngii and identify degradation tactics across various cultivation substrates. Thus, a comparative analysis of the secretory proteins based on Nanoliquid chromatography combined with tandem mass spectrometry was conducted among P. eryngii cultivated on sawdusts, bagasse, peanut shells, and glucose. In total, 647, 616, 604, and 511 proteins were identified from the four samples, respectively. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis of protein expression differences identified pathways (hydrolytic enzymes, catalytic activity, metabolic processes, cellular processes, and response to stimuli) significantly enriched in proteins associated with lignocellulose degradation in P. eryngii. An integrated analysis of proteome data revealed specifically or differentially expressed genes secreted by P. eryngii in different cultivation substrates. The most prevalent carbohydrate-active enzymes involved in lignocellulose degradation in the secretome of the four samples were laccase (Lac), manganese peroxidase (MnP), aryl alcohol oxidase (AaO), and copper radical oxidase (CRO). Among them, Lac 2 mainly involved in the lignin degradation of sawdust peanut shells, and bagasse by P. eryngii, and Mnp 3 was mainly involved in the degradation of peanut shells. AaO and Lac 4 were mainly involved in glucose substrate defense and oxidative stress. It was found that exogenous addition of sawdust and peanut shells significantly increased lignolytic enzyme abundance. These findings provide insight and guidance for improving agricultural waste resource recovery.In this study, the secretomes of P. eryngii grown on four different carbon sources were compared. The findings revealed the extracellular enzymes implicated in the degradation of lignocellulose, offering avenues for further investigation into the biotransformation mechanisms of P. eryngii biomass and the potential utilization of agricultural wastes. SignificanceThe cost of the substrate for mushroom cultivation has increased as the production of edible fungus has risen year after year. Therefore, the use of these locally available lignocellulosic wastes as substrates offers a cost-cutting option.Further, the overuse of wood for the cultivation of edible mushrooms is also detrimental to the conservation of forest resources or the ecological environment.Consequently, the use of other agricultural wastes as an alternative to sawdust or other woody substrates is a viable approach for cultivating P. eryngii.The distribution of extracellular lignocellulosic degrading enzymes, inferred in the present study could help improve the cultivation efficiency of P. eryngii vis-à-vis managing agricultural waste.

Recent advances on environmentally sustainable valorization of spent mushroom substrate: A review

AbstractCommercial cultivation of edible mushrooms utilizes a large amount of lignocellulosic material as a substrate. After harvest, the leftover substrate, referred to as a spent mushroom substrate (SMS), largely remains biochemically unaltered. In light of the global rise in the number of edible mushroom production facilities, it is pertinent to sustainably manage the by‐products of the mushroom production process, particularly SMS. Following the principles of the circular economy, SMS has shown potential for a variety of applications: SMS can be used as substrate for a new cycle of mushroom cultivation or as animal feed, fertilizer, soil amendment, bioremediation agent or a substrate for renewable energy production such as biogas, bioethanol, biohydrogen, solid biofuel, bio‐crude and bio‐oil. This article summarizes the current state of knowledge in various applications, highlights recent developments in the field, discusses potential barriers and offers suggestions for the long‐term growth of the mushroom industry worldwide.

Exploration of Practical Teaching Issues in the Course of Edible Mushroom Cultivation in Local Applied Universities

Exploration of Practical Teaching Issues in the Course of Edible Mushroom Cultivation in Local Applied Universities

Compatibility Analysis of Mushroom with different Botanicals in In vitro Condition

White button mushroom [Agaricus bisporus (Lange) Imbach] is the most popular cultivated edible mushroom, fetching high price and still dominating in Indian and International market. However, the limiting factor for its successful cultivation is the occurrence of competitor moulds. The present investigations were carried out in the laboratory Department of Plant Pathology, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, Uttar Pradesh. In this experiment different botanical (seed extract) viz., Trachyspermum ammi (Ajwain), Foeniculum vulgare (Saunf), Anethum graveolens (Soa), Trigonella foenumgraecum (Methi) were evaluated to test their compatibility with Agaricus bisporus. The botanicals (seed extract) were evaluated in vitro through poison food technique at 10, 20 and 30% concentrations and 24, 48 & 72 hours of incubation. The maximum radial growth of Agaricus bisporus was observed in T0-Control (Agaricus bisporus) (16.45mm), (17.12 mm) and (17.22 mm) and minimum in T1 - Trachyspermum ammi (Ajwain) (1.46mm), (0.83mm) and (0.60mm) at 10, 20 and 30 percent concentration respectively. Maximum percentage of inhibition was observed in T1 - Trachyspermum ammi (Ajwain) (91.11%), (95.13%) and (96.50%) at 10, 20 and 30 percent concentration respectively.

ՄԱԿՐՈՍԿՈՊԻԿ ՍՆԿԵՐԻ ԿԻՐԱՌՄԱՆ ՀԵՌԱՆԿԱՐՆԵՐԸ ԿՈՍՄԵՏՈԼՈԳԻԱՅՈՒՄ/PERSPECTIVES OF USAGE OF MACROFUNGI IN COSMETOLOGY

Macroscopic fungi, or macromycetes, which belong to the phylum Basidiomycota (class Agaricomycetes) and phylum Ascomycota (class Pezizomycetes), are well-known for their high nutritional value and pharmacological properties. Mycelium and fruiting bodies of wild and cultivated edible and medicinal mushrooms, as natural source of bioactive compounds (β-glucans, terpenoids, phenolics, alkaloids, thiamine, riboflavin, niacin, proteins, melanin, selenium, etc.) with medicinal and cosmetic effects (antiinflammatory, immunomodulatory, antimicrobial, antioxidant, anti-radiation, regenerating, moisturizing, nourishing, etc.) are widely used to develop various biotech products. Presently, edible and medicinal mushrooms from different taxonomic and ecological groups are of great interest in the production of anti-aging cosmetic products. Currently, 19 species of mushrooms , as potential source of cosmetic bioingredients were identified in the territory of Armenia. The study of mushroom resources, the creation and storage of pure cultures of mushroom collections open up wide opportunities for their biotechnological cultivation and the development of mycocosmetology in Armenia.

Interspecies hybridization between Auricularia cornea cv. Yu Muer and Auricularia heimuer cv. Bai Muer through protoplast fusion.

Color variations in cultivated edible mushrooms present novel and potentially valuable alternatives to the research and cultivation industries. We collected, identified, and domesticated a white strain of Auricularia cornea and a white strain of Auricularia heimuer from China. However, due to an unstable phenotype and stricter requirements on environment and management technology, the production and utilization of Auricularia heimuer cv. Bai Muer make slow progress. Outcrossing is an essential means to broaden the intraspecific genetic resources to expand the gene pool and compensate for the limitations of related species hybridization. In this study, interspecies hybridization between Auricularia cornea cv. Yu Muer and Auricularia heimuer cv. Bai Muer was conducted using polyethylene glycol (PEG)-induced double-inactivated protoplast fusion. Apart from the functional complementation of double-inactivated protoplasts, the hybrids were characterized by colony morphology, antagonistic test, primordial morphology, and polymerase chain reaction (PCR) fingerprinting. The results suggested that the hybrids and their parents showed significant differences in their colony morphology. Moreover, positive barrage reactions were observed between each parent and hybrid. Inter-simple sequence repeat (ISSR) and start codon targeted (SCoT) profile analysis of fusants and parents depicted that fusants contained polymorphic bands, which indicated the rearrangement and deletion of deoxyribonucleic acid (DNA) in the fusants. Yellowish-white primordia were obtained from two hybrids. Protoplast fusion may reinforce the genetic potential and provide an ideal alternative for breeding albino Auricularia.

Edible mushrooms – perspectives and considerations

The cultivation of edible mushrooms has been an activity of great ecological, social and economic importance for many years. There is a great diversity of different species of these mushrooms, truffles, chanterelles, pine mushrooms, Termitomyces, with more than 1100 registered species, many of which are of great economic importance not only for export but also for developing countries. The collection and consumption of these mushrooms vary by country, being, for example, extensive and intensive in China and more restricted in South American countries. The ecological value they have should also be highlighted, since some species live in symbiosis with trees, helping the growth of forests and commercial plantations. These fungi contribute to human nutrition supplying micronutrients, polysaccharides, amino acids, fibers and proteins; also, they synthesize active secondary metabolites such as saponins, glycosides, alkaloids, flavonoids, tannins, polyphenols and some other reducing compounds. This review about edible mushrooms addresses the generalities and production, description of several species, some toxic species in Mexico and finally several aspects of these mushrooms’ biotechnology.

Integration of white rot mushroom cultivation to enhance biogas production from oil palm kernel pulp by solid-state digestion

Solid-state fermentation is one of the promising technologies for biogas production because of its low water footprint and solid output which is potentially used in fuel or agricultural applications. Oil palm kernel pulp (OPKP) is a by-product generated from the extraction of palm kernel oil from the mesocarp of the oil palm tree and usually contains a large amount of lignocellulose and moderate protein content, which makes it suitable for use as a mushroom substrate. Cultivation of white rot mushrooms on lignocellulose may enhance its biodegradation by biodelignification. In this study, the incorporation of the cultivation of edible white rot mushrooms, Pluerotus ostreatus and Pleurotus pulmonarius, to enhance biogas production by solid-state digestion was studied. The biological efficiency of mushroom production from the OPKP substrate of P. ostreatus and P. pulmonarius was 49.81% ± 11.28% and 46.94% ± 13.49%, respectively, corresponding to the substrate weight loss of 15.87% and 13.92%. After 30 days, methane yield obtained through the solid-state digestion of P. ostreatus- and P. pulmonarius-treated OPKP substrates was increased to 98.11 mL/gVS (191%) and 101.10 mL/gVS (197%), respectively, compared with the untreated OPKP substrate. In consideration of energy loss during the biological conversion, the calorific values of the OPKP substrate, P. ostreatus-treated OPKP substrate, and P. pulmonarius-treated OPKP substrate were 11.03 ± 0.71 kJ/g, 9.30 ± 0.23 kJ/g, and 8.83 ± 0.70 kJ/g, respectively, while those of the digestion residues of P. ostreatus and P. pulmonarius-treated OPKP substrates were 8.45 ± 0.13 kJ/g and 8.55 ± 0.11 kJ/g, respectively.

Production of oxidative and hydrolytic enzymes from Pleurotus florida and their implication in mushroom cultivation

One of the most economically viable processes for the bioconversion of many types of lignocellulosic wastes is represented by edible mushroom cultivation. Pleurotus florida is one of the prominent economically cultivated mushrooms with varied abilities to utilize lignocellulose as a growth substrate. Thus, the profile of oxidative and hydrolytic enzymes was measured during the submerged and solid-state fermentation at Punjab Agricultural University, Ludhiana, Punjab during the winter (rabi) season of the year 2020–21. The intracellular activity of laccase (10.23 IU/mg), manganese peroxidase (9.98 IU/mg), cellobiohydrolase (5.46 IU/mg) was best obtained in P. florida on the 14th day. The extracellular activity of laccase (15.43 IU/ml), lignin peroxidase (11.21 IU/ml), endoxylanases (9.22 IU/ml)were found to be highest on the 14th day. P. florida had substantial levels of ligninolytic enzymes during substrate colonization but rapidly diminished during fruit body development. On the other hand, P. florida showed relatively low hydrolase activity during substrate colonization. The activity of these enzymes increased when primordia formed. The hydrolytic activities peaked at the mature fruiting body stage. When mushrooms switched to vegetative development, ligninolytic enzyme activity increased again, while hydrolase activity declined. The yield was found to be 58.78% after the enzymatic treatment of paddy straw. These findings suggest that fluctuations in oxidative and hydrolytic enzyme activities are linked to the growth of the fruiting body and the physiological state of mushroom cultivation.

Applications and efficacy of traditional to emerging trends in lacto-fermentation and submerged cultivation of edible mushrooms

Applications and efficacy of traditional to emerging trends in lacto-fermentation and submerged cultivation of edible mushrooms

Effects of Mineral Composition of Different Wood Wastes in Ibadan on Proximate Composition of Lentinus Squarrosulus (Mont.)

Lentinus squarrosulu (Mont.) is one of the cultivated indigenous edible mushrooms that are available in Nigeria. Recently, there has been growing crave to crop wild edible mushroom species that have potential economic values. Mushrooms can be an alternative source of protein especially for commoners in the society. Hence, the objective of this paper is to evaluate the effect of mineral composition of five wood wastes- Triplochiton scleroxylon, Cordia milleni, Vitellaria paradoxa, Pouteria altissima and Anogeissus leiocarpa different wood wastes on L. squarrosulus (Edible Mushroom) cultivation for their consequences on the growth, yield and proximate analysis. Data obtained reveal Carbon/Nitrogen ratio of 11.59, composition of Magnesium (0.035 mg), Potassium (0.053 mEq/l), Manganese (0.0013 mg), Copper (0.00050 g/m3), Iron (0.00275 mol/L), Phosphorus (0.027 mmol/L), Organic carbon (32.10 mg/L C), Organic matter (55.3 t/ha) and total nitrogen (2.77 mg/L) contributed greatly to the high crude protein, fats and ash contents of mushroom cultivated on T. Scleroxylon. However, insignificant contents of sodium (0.2 mg), Calcium (0.2 mmol/L) and Magnesium (0.013 mg) in Pouteria altissima led to the general inadequate performance of L. squarrosulus in yields. The percentage fresh mean weight of L. squarrosulus ranged from 9.13 g – 19.00 g. The highest value was produced by T. scleroxylon followed by P. altissima, V. paradoxa, C. milleni while A. leiocarpa gave the least.

The transcription factor Ste12-like increases the mycelial abiotic stress tolerance and regulates the fruiting body development of Flammulina filiformis.

Flammulina filiformis is one of the most commercially important edible fungi worldwide, with its nutritional value and medicinal properties. It becomes a good model species to study the tolerance of abiotic stress during mycelia growth in edible mushroom cultivation. Transcription factor Ste12 has been reported to be involved in the regulation of stress tolerance and sexual reproduction in fungi. In this study, identification and phylogenetic analysis of ste12-like was performed by bioinformatics methods. Four ste12-like overexpression transformants of F. filiformis were constructed by Agrobacterium tumefaciens-mediated transformation. Phylogenetic analysis showed that Ste12-like contained conserved amino acid sequences. All the overexpression transformants were more tolerant to salt stress, cold stress and oxidative stress than wild-type strains. In the fruiting experiment, the number of fruiting bodies of overexpression transformants increased compared with wild-type strains, but the growth rate of stipes slowed down. It suggested that gene ste12-like was involved in the regulation of abiotic stress tolerance and fruiting body development in F. filiformis.

A comparative study of fatty acid compositions of three cultivated edible mushroom species of Bangladesh

A comparative assessment of three cultivated edible mushroom species namely Bottom mushroom (Agaricus bisporus), Pearl Oyster (Pleurotus ostreatus), Shiitake Mushroom (Lentinul aedodes) on the cumulative fatty acid compositions through fatty acids methyl esters (FAMEs) was devised. About Thirty-six fatty acids were distinguished in the selected mushroom species to interpret descriptive and analytical information by deriving all free fatty acids to their corresponding FAMEs utilizing gas chromatography-mass spectrometry (GC-MS/MS). Cis-linoleic acid was the predominant fatty acid in the fruit bodies of all studied mushrooms. The range of cis-linoleic acid content in the species was 40.33% to 65.59%. Cis-oleic and heptadesenoic acids, respectively, were the other two main fatty acids. More than 32 fatty acids were found and quantified in addition to the three primary fatty acids that are already mentioned. The sequences of Polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA) and saturated fatty acids (SFA) within all saturated and unsaturated fatty acids for three mushroom species were PUFA > MUFA > SFA for Agaricus bisporus and Lentinula edodes while the sequence was PUFA > SFA > MUFA for Pleurotus ostreatus. Lentinula edodes presented the highest fatty acids amounts which were followed by Pleurotus ostreatus and Agaricus bisporus respectively. The fatty acid composition of the mushrooms revealed that the unsaturated fatty acids are present in higher amounts than the saturated fatty acids in the analyzed mushrooms.