Cultivar IAC Research Articles

Responsiveness of Arabica coffee cultivars to skeleton pruning in a low‐altitude region

AbstractSkeleton pruning is a type of pruning that, by cutting lateral branches (plagiotropic), allows the reinvigoration of coffee plants through the renewal of productive branches. The crop response to this management may be affected by several factors, such as the trees age, crop practices, and, mainly, the genotype. This study aimed to identify the Arabica coffee cultivars with greatest responsiveness to skeleton pruning and desirable productive and qualitative characteristics in a low‐altitude region. The experiment was conducted in northeastern São Paulo state, Brazil, at an altitude of 565 m. The experimental design was randomized blocks with four replicates, and the treatments consisted of 17 short‐stature Arabica coffee cultivars. On average, the responsiveness of the cultivars to pruning was 32% in the first year and 14% in the second year. The cultivars IAC Catuaí SH3, IAC Ouro Verde, IAC Obatã 4739, Tupi IAC 1669‐33, Catiguá MG1, Oeiras MG 6851, Pau‐Brasil MG1, Sacramento MG1, IPR 99, IPR 103, and Sabiá Tardio were responsive to skeleton pruning. Among these, Catiguá, Oeiras, Sacramento, and Sabiá stood out with pruning responsiveness above 45%. The cultivars Obatã IAC 4739, Tupi IAC 1669‐33, and Catuaí SH3 stood out in terms of yield, with an average between 40 and 50 bags ha−1. In addition to high yields, the cultivar Obatã IAC 4739 obtained grains with higher weight and sieve 17. Skeleton pruning was found to be genotype dependent and emerged as a viable alternative for revitalizing Arabica coffee cultivars in low‐altitude regions.

Biomass yield and chemical composition of the cassava plant over two vegetative growth cycles

The objective of this work was to study the biomass production and chemical composition of the different parts of the cassava plant during the first and second vegetative cycles, with the aim of providing data that can contribute to the understanding of the response of cassava cultivars to different harvest ages. The experimental design consisted of randomized blocks in split plot scheme. Plots were represented by cultivars IAC 90 and IAC 118-95 and subplots by crop age (2, 4, 6, 8, 10, 12, 14, 16, and 18 months after planting). Our results showed that the ‘IAC 90’ allocated a higher proportion of assimilates to the leaves, stem and planted cutting than the ‘IAC 118-95’, which was more efficient in allocating dry matter to the storage roots. Storage roots showed an increase of more than 50% in starch content 14 months after planting. The cultivar IAC 118-95 is distinguished by the higher harvest index, allowing earlier harvesting, with possible valorisation of the leaves as industrial raw material. Variables showed different degrees of inter-relationships amongst themselves. Total plant fresh matter and dry matter yields were positively correlated with growth parameters and root starch for both cultivars. Harvest time and cultivar are key factors that should be considered to increase profits in the cassava agro-industrial chain.

Application of Bacillus spp. Phosphate-Solubilizing Bacteria Improves Common Bean Production Compared to Conventional Fertilization.

The use of phosphate-solubilizing bacteria (PSB) can be a sustainable strategy to increase phosphorus availability and promote satisfactory crop yields. The objective of this study was to evaluate whether inoculation with PSB in common bean increases (i) growth, (ii) nutrition, (iii) yield, and (iv) grain quality, and (v) reduces the chemical phosphorus application dose to obtain maximum yields. The experiment was conducted in an Oxisol using a randomized block design in a 4 × 4 factorial scheme, with four replicates, using the cultivar IAC 2051. The first factor was four doses of P2O5 (0, 20, 40 and 60 kg ha-1), and the second factor was four doses of PSB (0, 100, 200 and 300 mL ha-1). For leaf area and leaf chlorophyll content, the association of PSB inoculation with a P2O5 dose of 40 kg ha-1 promoted the best conditions for the common bean. P2O5 application increased yield by 79 kg ha-1 for each 10 kg ha-1 added. PSB inoculation at a dose of 192 mL ha-1 promoted P export of 15.3 kg ha-1, and the PSB dose of 159 mL ha-1 increased yield by 389 kg ha-1 (12%) compared to the control. Grain quality remained within the standards required by the consumer market, being little affected by the treatments. Improvements in common bean growth and nutritional and physiological status promoted by P2O5 application and PSB were essential in increasing yield, so these are sustainable production strategies.

What are the impacts of water deficit, cultivars, and agricultural years on the dynamics of nutrient uptake by common bean? Part I: n, P, and K

Knowing the effect of water deficit intensity on the nutrient uptake dynamics by common bean cultivars with contrasting growth habits in different years is necessary to appraise and recommend more specific fertilization management practices. A field study was carried out in the Southeast of Brazil for two years aiming to explain and compare the accumulation and extraction of the macronutrients nitrogen (N), phosphorus (P), and potassium (K) from two common bean cultivars with contrasting growth habits as a function of water deficit severity. The common bean cultivars IAC Imperador and IPR Campos Gerais, with determinate and indeterminate growth habits, respectively, were evaluated. Both cultivars were submitted to three irrigation management practices: severe water deficit (54% of the crop evapotranspiration – ETc), moderate water deficit (77% of ETc), and no water deficit (100% of ETc). Overall, the severe water deficit reduced N, P, and K accumulation in the common bean cultivars by 26, 37, and 23%, respectively. Under no water deficit, IPR Campos Gerais showed higher accumulation of biomass and nutrients N (27%) and P (42%) compared to IAC Imperador, in addition to higher interannual variability in the accumulation of nutrients. The maximum daily demand for nutrients N, P, and K of IAC Imperador was anticipated in 9, 13 and 11 days, respectively, compared to IPR Campos Gerais. Irrigation management, cultivar, and climate of the agricultural year are important factors in decision-making for the most adequate establishment of fertilization with N, P, and K in common bean.

Increased hormetic dose of glyphosate causes oxidative stress and reduces yield in common bean

Glyphosate can alter several physiological and biochemical processes and even plant nutrient absorption. This study aimed to verify whether biochemical and non-biochemical variables of the antioxidant complex are affected by low doses, considered hormetic, of glyphosate herbicide. Two experiments were conducted in the field, one in the winter and the other in the wet season, with the early cycle common bean cultivar IAC Imperador. The experimental design was in a randomized block, consisting of applying low doses of glyphosate (0.0, 1.8, 7.2, 12, 36, 54, and 108 g a.e. ha–1) in the phenological stage V4, with four replicates. Environmental conditions, such as air temperature, interfered in the early cycle common bean response to low doses of glyphosate. Lower doses of glyphosate did not alter the gas exchange of bean plants. However, the increase in the glyphosate doses reduced the net CO2 assimilation rate, stomatal conductance, and transpiration rate while increasing the SOD and CAT activity, and MDA and proline content. In the winter season, the dose 7.2 g a.e. ha–1 increased bean productivity, while the dose 36 g a.e. ha–1 induced oxidative stress in bean plants and reduced productivity. In the wet season, doses 36 and 54 g a.e. ha–1 increased bean productivity, and the dose 108 g a.e. ha–1 decreased grain yield. Thus, the more favorable climatic conditions in the wet season enhance plant metabolism, subsequently increasing plant tolerance to higher glyphosate doses.

Marker-assisted introgression of wild chromosome segments conferring resistance to fungal foliar diseases into peanut (Arachis hypogaea L.).

Fungal foliar diseases can severely affect the productivity of the peanut crop worldwide. Late leaf spot is the most frequent disease and a major problem of the crop in Brazil and many other tropical countries. Only partial resistance to fungal diseases has been found in cultivated peanut, but high resistances have been described on the secondary gene pool. To overcome the known compatibility barriers for the use of wild species in peanut breeding programs, we used an induced allotetraploid (Arachis stenosperma × A. magna)4x, as a donor parent, in a successive backcrossing scheme with the high-yielding Brazilian cultivar IAC OL 4. We used microsatellite markers associated with late leaf spot and rust resistance for foreground selection and high-throughput SNP genotyping for background selection. With these tools, we developed agronomically adapted lines with high cultivated genome recovery, high-yield potential, and wild chromosome segments from both A. stenosperma and A. magna conferring high resistance to late leaf spot and rust. These segments include the four previously identified as having QTLs (quantitative trait loci) for resistance to both diseases, which could be confirmed here, and at least four additional QTLs identified by using mapping populations on four generations. The introgression germplasm developed here will extend the useful genetic diversity of the primary gene pool by providing novel wild resistance genes against these two destructive peanut diseases.

Response of early-cycle common bean cultivars with carioca, black and speckled grains to top-dressing nitrogen fertilization

The aim was to evaluate the agronomic and qualitative attributes of early-cycle common bean cultivars with different grains types grains in response to top-dressing nitrogen (N) doses. The experiment was carried out using a randomized block design, in a split-plot scheme, with 4 replicates. The plots consisted of the cultivars IAC Nuance, IAC 1849 Polaco and IAC Veloz, with speckled, Carioca and black grains, respectively. The subplots were formed by N doses applied as top-dressing: 0 kg ha-1, 60 kg ha-1 (applied in the stage of third trifoliate leaf), 120 kg ha-1 (1/2 applied at third trifoliate leaf stage + 1/2 applied at the floral bud stage) and 180 kg ha-1 (1/3 applied at the first trifoliate leaf stage + 1/3 applied at the third trifoliate leaf stage + 1/3 applied at the floral bud stage). IAC Veloz stood out for grain yield, showing the highest grain yield in the lowest N doses, being classified as efficient to the use of N. The cultivars IAC Nuance and IAC 1849 Polaco reached maximum yields with 155 and 163 kg ha-1 of N. The IAC Nuance was the most responsive, increasing grain yield by up to 25.3% due to nitrogen fertilization. Increasing N doses applied as top-dressing increased the sieve yield and crude protein content of the common bean cultivars, with IAC Nuance standing out. The cultivars showed good grain quality, and IAC 1849 Polaco and IAC Veloz had the shortest cooking time and IAC Veloz also had the fastest hydration.

Irrigation management of common bean cultivars with contrasting growth habits

A study was undertaken comparing the water requirements of two common bean ( Phaseolus vulgaris L.) cultivars to generate specific recommendations aimed at optimizing water use. To accomplish this work, the agronomic performance, responsiveness to water and water productivity of these two common bean cultivars of determinate and indeterminate growth habits were identified. The 2-year experiment was carried out during the winter growing season in the southeast of Brazil. Cultivars IAC Imperador, with an early season of determinate growth habit, and IPR Campos Gerais, having a mid-season of indeterminate growth habit, were subjected to five irrigation levels (54, 70, 77, 100, and 132 % of the crop evapotranspiration). Water deficit affected agronomic performance, reducing plant height (by up to 29 %), leaf area index (by up to 40 %), soil cover fraction (by up to 28 %), and grain yield (GY - by up to 31 %), in both cultivars. In contrast, excess water was more detrimental to cultivar IAC Imperador. Cultivar IPR Campos Gerais produced 18 % more than GY, showing superior water productivity and response to irrigation depth than IAC Imperador. Out of all the variables evaluated, the soil cover fraction correlated the most with grain yield in both common bean cultivars during the 2-year study. In other words, cover fraction evaluation in common bean allows for estimating crop production potential, which helps producers and technicians in their decision making regarding management practices. Thus, a cultivar directly affects water use in common bean production, thereby suggesting the need for a or water conservation strategy and sustainability of irrigated common bean production.

Yield response to row spacing, irrigation and varieties of peanut in the semiarid region of Minas Gerais, Brazil

Studies on the management of peanut crops in the semiarid region of Minas Gerais are scarce. Therefore, the objective was to evaluate the agronomic performance of four peanut cultivars under different irrigation systems and row spacing in northern Minas Gerais. The cultivars IAC 503, Runner IAC 886, IAC Tatu ST, and Crioula were grown at row spacing of 0.5, 0.7, and 0.9 m and irrigated or not irrigated. The following variables were evaluated: Total number of pods, number of commercial pods, number of seeds, mass of one hundred grains, grain yield index, oil content, pod yield, grain yield and oil yield. After analysis of variance, the average values were compared using the Scott-Knott method with a probability of 5%. The spacing of 0.5 m promoted higher productivity in pods, grains, and oil in both systems. Under irrigation, IAC 503 and IAC 886 were the most productive, while the cultivars did not differ under non-irrigated cultivation. Finally, irrigation resulted in an increase in grain yield of up to 50% compared to non-irrigated cultivation.

UFSM XIKA FW: sugarcane cultivar for southern Brazil

In Brazil, in view of the different soil and climatic conditions of the sugarcane-producing regions, a strategy of creating small breeding programs for the crop is being used. Since 2008, the sugarcane breeding program of the Federal University of Santa Maria (UFSM), has been developing new, better adapted and higher-yielding sugarcane cultivars for the climate conditions of the southern region of Brazil. Cultivar UFSM XIKA FW was developed at the Laboratories of Agroclimatology and Genetic Breeding and Plant Production of the UFSM, Campus Frederico Westphalen. It is the first mutation-induced sugarcane cultivar that was protected by the Ministry of Agriculture, Livestock and Food Supply, Brazil. The genotype used for mutation induction was cultivar IAC 87-3396, due to its good performance characteristics in the southern region. The mutation mechanism was induced in 2011, by immersing individual buds in a solution of the chemical mutagenic agent methyl methane sulfonate. The best genotypes were selected and tested in plant cane in 2013 and 2018 and in ratoon cane trials in 2014 and 2015. The new cultivar has a higher sugar production potential, and the mean yield of four years was 8.2 t ha-1 higher than that of the control, indicating a high yield potential.

Competitive ability of bean cultivars with Urochloa plantaginea

The objective of this work was to evaluate the competitive ability of carioca bean cultivars BRS FC104, BRS FC402, IAC Imperador, IAC Milênio, IPR Tangará, and SCS Riqueza in the presence of Alexandergrass (Urochloa plantaginea) in different proportions of plants in association. The experiments were carried out in a greenhouse in a completely randomized design with four replications. The treatments were arranged according to the proportions of beans and Alexandergrass plants: 100:0, 75:25, 50:50, 25:75, and 0:100 or 40:00, 30:10, 20:20, 10:30, and 0:40 plants per pot. The competitiveness of the species was analyzed using diagrams applied to replacement experiments and also through relative competitiveness indices. At 30 days after crop emergence, leaf area (LA), height (HP), diameter (DP), and shoot dry mass (SM) of the plant shoots were measured, as well as variables related to the physiology of the species. There was competition among carioca bean cultivars in the presence of Alexandergrass regardless of the proportion of plants, causing decreases in DP, LA and SM. Basically, there is competition for the same environmental resources between beans and Alexandergrass. Highlights: There is competition between bean and Alexandergrass, for the same environmental resources; There is varietal difference - IAC Imperador and SCS 205 Riqueza are the most competitive ones; The escape to competition is the best approach to guarantee yield in Alexandergrass-infested fields.

Potential parents of carioca bean for use in breeding aiming resistance to Colletotrichum lindemuthianum

AbstractCarioca common bean (Phaseolus vulgaris L.) is the most produced and consumed in Brazil. The production of carioca bean is affected by fungal diseases such as anthracnose, caused by the fungus Colletotrichum lindemuthianum. Most sources resistant to anthracnose, used in crosses by breeding programs are not derived from the commercial group or are poorly productive and less adapted. Thus, the objective of this study was to characterize 40 carioca bean cultivars already recommended in Brazil for their reaction to C. lindemuthianum races 65, 73, 81, and 89. The cultivars and the resistant control, BRS Esplendor, were inoculated in a greenhouse with two different experiments for each race. Seedlings with fully expanded primary leaves were inoculated with a suspension of each pathogen race and assessed after 12 d. Among the 40 cultivars assessed, 12.5, 2.5, 12.5, and 15% resistant to one, two, three, and four races of C. lindemuthianum, respectively. The cultivars IAC Formoso, IAC‐Carioca Pyatã, IAC‐Apuã, VC 15, BRS Notável, and IPR Campos Gerais were resistant to the four races, constituting parental sources of resistance to the pathogen. In regions that favor the occurrence of anthracnose in common bean, cultivars with certain levels of resistance should be selected. Therefore, the important information obtained will be made available to growers and those offering technical assistance to growers.

PARAMETERS AND GENETIC VARIABILITY IN BLACK BEANS F5 FAMILIES

The objective of this work was to contrast expectations of the segregating generations F2, F3, F4, and F5 with homozygous genetic constitutions, to determine the genetic parameters, dissimilarities through multivariate models, and the associations between characters of agronomic importance for the breeding of black beans. The experiment was conducted in the 2017/2018 crop season in the county of Tenente Portela – RS, Rio Grande do Sul, Brazil. The experimental design used was that of augmented blocks, with the cultivars IAC Imperador, IPR Tangará, BRS Estilo composing the common treatments, considered as pure lines, and 131 lineages of the F5 segregating generation, composing the unusual treatments, arranged in three blocks. After the characterization of the 134 genotypes, it was possible to verify that through Singh's relative contribution, the character of number of seeds per plant was the most decisive for the genetic variability of this study. The use of biometric methods allows it to be possible to evidence patterns in relation to the genotype clusters, through the use of standardized average Euclidean distance patterns, Tocher's optimization method, UPGMA clustering method, and Artificial Neural Networks with unsupervised learning. With the results obtained, it was possible to verify the existence of genetic variability among the studied populations, allowing the cluster analysis to delimit

Comparison of chemical and nutritional compositions between aromatic and non-aromatic rice from Brazil and effect of planting time on bioactive compounds

The objective of this work was to investigate and characterize new rice cultivars still little explored and never reported in the literature but of nutritional and economic potential. For this, the Brazilian cultivars IAC 500 (aromatic) and IAC 2157 (non-aromatic) were analyzed and compared regarding their proximate composition, amylose content, fatty acid composition, vitamin E content, and glycemic and insulinemic responses. An additional goal of the work was to analyze the influence of the planting month on the contents of minerals, phytosterols, and free phenolic compounds from the cultivars planted under the same conditions at four different months of the year. This work presents the first report of the chemical characterization of the Brazilian aromatic rice IAC 500, that present higher ash, protein, lipid, α-tocopherol, and unsaturated fatty acid levels compared with the Brazilian non-aromatic rice IAC 2157. The planting time quantitatively influenced the bioactive compounds, but there was no consensus regarding the best planting time to maximize the content of the analyzed compounds. The main finding of the study is that the ingestion of aromatic rice resulted in a lower insulinemic response compared to non-aromatic rice. Future research is needed to investigate the observed difference in pancreatic insulin release after consumption of aromatic rice.

What are the impacts of water deficit, cultivars, and years on the dynamics of nutrient uptake by common bean? Part II: Ca, Mg, and S

We aimed to explain and compare Ca, Mg, and S uptake dynamics by two common bean cultivars with contrasting growth habits as a function of water deficit severity in two years to generate more specific information for their fertilization management. A field study was set up using the cultivars IAC Imperador, with a determinate growth habit and early cycle, and IPR Campos Gerais, with an indeterminate growth habit and intermediate cycle. These cultivars were maintained under three irrigation levels, that is, severe (54% ETc), moderate (77% ETc), and no water deficit (100% ETc). The absorption order was Ca > Mg > S, regardless of the treatment. For the IAC Imperador, the Ca uptake ranged from 72 to 127 kg ha−1, Mg from 17.2 to 39.1 kg ha−1 and S from 7.9 to 16.5 kg ha−1, while for the IPR Campos Gerais it ranged from 92 to 156 kg ha−1, 21.0 to 36.5 kg ha−1 and 14.6 to 33.7 kg ha−1, respectively. Overall, severe water deficit reduced Ca, Mg, and S absorption by more than 25%. The IPR Campos Gerais presents, on average, 64% higher S accumulation, while the highest Ca and Mg accumulation order depends on the year. Compared to the IAC Imperador, this cultivar showed a later S absorption peak (up to +23 days), and higher interannual variability in the growth and nutrient absorption due to its indeterminate growth habit. These differences evidence the importance of specific fertilizer management practices for Ca, Mg and S in common bean, assisting in optimizing nutrient use.

Physiological potential and health of peanut seed treated with ozone

Peanut seed can be infected by pathogen diseases that adversely affect the oil crop’s value chain. Ozone gas (O3) can be an alternative for the control of fungal diseases due to its high oxidizing potential. In this study, we investigated the effects of O3 exposure time on physiological and health characteristics of seed from three peanut cultivars. Seed from peanut cultivars Granoleico, IAC OL3, and IAC 503 were analyzed for their health (blotter test) and physiological potential (germination, first count, and seedling length) after exposure to O3 for 0, 1, 8, and 16 h. Peanut seed physiological quality and health differed among cultivars. Exposure of seed from peanut cultivars IAC OL3, IAC 503, and Granoleico to O3 at a rate of 600 mg h-1 did not affect the development of normal seedlings as measured by the first count and germination, but seedling length increased, decreased, or was not affected in cultivars Granoleico, IAC 503, and IAC OL3, respectively. Also, O3 did not eradicate fungal infections in peanut seed treated with O3 at a rate of 600 mg h-1 for up to 16 h, but the incidence of the fungus Rhizopus sp. in seed from cultivars IAC OL3, Granoleico, and IAC 503 increased, decreased or was not affected, respectively, after 8 h.

Estimate and Temporal Monitoring of Height and Diameter of the Canopy of Recently Transplanted Coffee by a Remotely Piloted Aircraft System

Digital agriculture is fundamental to potential improvements in the field by optimizing processes and providing intelligent decision making. This study aims to calculate the height and canopy diameter of recently transplanted coffee plants over three periods of crop development using aerial images, verify statistical differences between field measurements and aerial images, estimate linear equations between field data and aerial images, and monitor the temporal profile of the growth and development of the cultivar understudy in the field based on information extracted from aerial images through a Remotely Piloted Aircraft System (RPAS). The study area comprises a recently transplanted five-month-old Coffea arabica L. cultivar IAC J10 with information of height and crown diameter collected in the field and aerial images obtained by RPAS. As a result, it was possible to calculate the height and diameter of the canopy of coffee plants by aerial images obtained by RPAS. The linear estimation equation for height and crown diameter was determined with satisfactory results by coefficients R and R2 and performance metrics MAE, RMSE, and regression residuals, and it was possible to monitor the temporal profile of the height of the coffee cultivar in the field based on aerial images.

Soil enzymatic activity under coffee cultivation with different water regimes associated to liming and intercropped brachiaria

ABSTRACT: This research evaluated the effects of coffee cultivation with two different water regimes associated or not with liming and the presence/absence of brachiaria as intercrop on the activities of the soil enzymes β-glucosidase, arylsulfatase and acid phosphatase. The study was carried out at the experimental farm of Embrapa Cerrados, using the cultivar IAC 144 (Coffea arabica L.), under a clayey dystrophic Cerrado Oxisol. Two water regimes (WR) were considered, WR1 with irrigation shifts throughout the year and WR3 with controlled water stress, for about 70 days, in the dry season. In each water regime, effects of lime application (with/without) and the presence/absence of brachiaria cultivated between the lines of coffee plants were evaluated. The activities of the enzymes β-glucosidase, arylsulfatase and acid phosphatase were evaluated during the rainy and dry seasons. Liming and intercropped brachiaria positively affected the activities of the three enzymes assessed in this study at varying degrees, depending on season and/or the WR. Our findings evidenced that intercropped brachiaria in coffee rows was the factor that most positively impacted soil enzymes activities.

Número de sementes em frutos e frequência de híbridos obtidos de cruzamentos com a tangerina IAC 2019 Maria

Abstract The Citrus Breeding Program of the Citriculture Center at the Agronomic Institute (IAC) has developed a mandarin cultivar IAC 2019Maria from the crossing between Murcott IAC tangor (Citrus reticulata x Citrus sinensis) and Pera IAC sweet orange (C. sinensis). The present study aimed to assess the number of seeds in fruits of IAC 2019Maria mandarin and to identify zygotic embryos and triploid plants in the crossings between IAC 2019Maria mandarin, Pera IAC sweet orange, and Ponkan mandarin (C. reticulata Blanco), in addition to IAC 2019Maria mandarin in open and self-pollination. IAC 2019Maria flowers were self-pollinated, pollinated with pollen from Pera sweet orange and Ponkan mandarin, and had no pollination. The embryos were identified using microsatellite molecular markers and ploidy was assessed by flow cytometry. The results of the treatment with no pollination suggest the variety does not produce parthenocarpic fruits. The genotyping results showed that 100% of the populations consist of zygotic embryos, suggesting that IAC 2019Maria mandarin is a plant with low polyembryony. The ploidy analysis of the hybrids allowed identifying a triploid plant from an aborted seed from the crossing with Pera sweet orange and two tetraploids, one from the crossing with Pera sweet orange and one from self-pollination.

Phytochemical evaluation of lipoxygenase-free soybean genotypes for human consumption

Abstract The present study aimed to screen genotypes for the presence/absence of lipoxygenases and to evaluate the phytochemical characteristics of lipoxygenase-free genotypes. Progenies derived from the cross between the cultivars IAC PL-2 (with lipoxygenases) and BRS 213 (lipoxygenase-free) generated 114 inbred lines in the F6/7 generation, evaluated by the qualitative colorimetric method, to verify the presence/absence of lipoxygenase isoenzymes L-1, L-2 and L-3. Eight triple-null (lipoxygenase-free) lines were confirmed by visible spectrophotometry. Progenitors and triple-null lines IAC 10-0003-1, IAC 10-0010-1, IAC 10-0010-2, IAC 10-0012, IAC 10-0043, IAC 10-0046, IAC 10-0107-1 and IAC 10-0115-2 were analyzed to assess their contents of protein, oil, phytic acid, tannins, total phenols and total flavonoids. Analyses showed significant differences in protein (from 38.80% to 41.89%) and oil (from 18.86% to 22.88%), consistent with the values observed in commercial soybean cultivars. The new triple-null lines, compared to the BRS 213 standard, showed lower contents of soluble condensed tannins; lower phytic acid in line IAC 10-0043 and greater amount of total phenols in line IAC 10-0115-2; whereas, the lines IAC 10-0010-1, IAC 10-0010-2, IAC 10-0043, IAC 10-0046 e IAC 10-0107-1 stood out with higher values of total flavonoids. The new lines will be potentially useful parents for genetic breeding and for a multiline strategy, aiming at greater phenotypic stability of lipoxygenase-free soybeans for human consumption.