As most outcropping and shallow mineral deposits have been found, new technology is imperative to finding the hidden critical mineral deposits required for the renewable energy transition. One such seismic technique, called ambient noise tomography (ANT), has shown promise in recent years as a low cost and low environmental impact passive method of three-dimensional (3D) imaging of shear-wave velocity structure of the Earth. Over the last twenty years the method has been well-established in academia to image crustal and regional scale geological features but has seldom been used at the mineral exploration deposit-scale. In this paper we show the application of seismic ANT at an IOCG deposit in South Australia under more than 750 m of sedimentary cover. A 100-site survey in a 10 by 10 grid with site spacing of 1 km, using 3-component nodal seismometers with a natural-frequency of 5 Hz, was conducted over a two-week period. Data were inverted to generate a 3D velocity model to a depth of 2 km. When compared to drillholes in the survey area, the tomographic model delineates cover sequence lithologies and the depth of crystalline basement. A velocity anomaly in the basement has characteristics of a potential IOCG deposit and is aligned with a gravity anomaly due to brecciated haematite. The results of the paper indicate that ANT is a useful tool for deep cover mineral exploration that can potentially expedite the discovery of new deposits.