Cruises In August Research Articles

First record of Pseudoterranova decipiens in sprat (Sprattus sprattus) from the Baltic Sea.

A dispersion of Anisakidae nematodes (particularly Contracaecum osculatum) among marine organisms in the Baltic Sea has been reported over the last decade. This is in line with an increase in the number of grey seal that act as final host for Contracaecum osculatum and Pseudoterranova sp., and are thus indispensable for the completion of their life cycles. Most attention has been paid to zoonotic nematode species, like Pseudoterranova sp., which have been noted in commercially important fish in the area. Little is known about the spread and transmission of Pseudoterranova sp. in the Baltic Sea. The aim of this study was to investigate whether sprat may play a role as a transport host for this Anisakidae. Samples were collected in three areas of the southern Baltic Sea (south and east of Bornholm, Słupsk Farrow and the Gulf of Gdańsk) during a research cruise in August 2019. A visual inspection of the viscera of 556 sprats was conducted. Parasites were identified using anatomomorphological and molecular methods. Nematodes were recorded only in sprat caught southeast of Bornholm (prevalence 2.7%; intensity of infection 1-4; abundance 0.05). Molecular identification revealed the presence of Pseudoterranova decipiens. This is the first report of P. decipiens in sprat from the Baltic Sea. Sprat is likely a transmitter of P. decipiens in the Baltic Sea food web.

Active gas seepage in western Spitsbergen fjords, Svalbard archipelago: spatial extent and geological controls

This study presents the first systematic observations of active gas seepage from the seafloor in the main fjords of western Spitsbergen in the Svalbard archipelago. High-resolution acoustic water column data were acquired throughout two research cruises in August 2015 and June 2021. 883 gas flares have been identified and characterized in Isfjorden, and 115 gas flares in Van Mijenfjorden. The hydroacoustic data indicate active fluid migration into the water column. Interpretation of 1943 km of regional offshore 2D seismic profiles supplemented the water column and existing gas geochemical data by providing geological control on the distribution of source rocks and potential migration pathways for fluids. In the study area, bedrock architecture controls the fluid migration from deep source rocks. Faults, high permeability layers, heavily fractured units and igneous intrusions channel the gas seepage into the water column. The observations of gas seepage presented in this study are an important step towards the assessment of how near-shore seepage impacts upon the carbon budget of Svalbard fjords, which constitute a globally recognized early climate change warning system for the High Arctic.

A river of terrestrial dissolved organic matter in the upper waters of the central Arctic Ocean

The Transpolar Drift (TPD) is a mechanism for the rapid transport of shelf-modified Siberian river waters across the Arctic Ocean and out through Fram Strait. We present results from three trans-Arctic research cruises in August–September 2014–2016 to investigate the distribution and dynamics of dissolved organic matter (DOM) in the upper waters across the North Pole, from Svalbard to the Mackenzie River mouth, and in two important outflow gateways of the Arctic Ocean (Fram Strait and Lancaster Sound). Fluorescence measurements of DOM obtained using in situ sensor and laboratory-based spectroscopic analyses, coupled with parallel factor analysis (PARAFAC) were used to map components of DOM at high spatial resolution. The optical properties showed a completely different composition with a much stronger humic-like signal observed in surface waters in the Makarov Basin than in the adjacent Canada Basin, likely due to the substantial amount of Siberian runoff transported by the TPD. The high levels of humic-like in TPD-influenced surface waters were traced from southern Makarov shelves up to the Lomonosov Ridge near the North Pole. Lower DOM levels were observed in the eastern Amundsen basin waters, likely the result of mixing with terrestrial-poor DOM Atlantic waters. The annual flux of humic-like C2 transported to the Atlantic through Fram Strait was estimated at 1816 ±926 r.u. km3. This study reveals the importance of shelf processes and TPD in supplying humic-like material to the central Arctic Ocean to Fram Strait.

Diversity and biogeography of planktonic diatoms in Svalbard fjords: The role of dispersal and Arctic endemism in phytoplankton community structuring

Understanding the processes that shape the community structure of Arctic phytoplankton is crucial for predicting responses of Arctic ecosystems to the ongoing ocean warming. In particular, little is known about the importance of phytoplankton dispersal by the North Atlantic Current and the prevalence and maintenance of Arctic endemism. We investigated the diversity and biogeography of diatoms from five Svalbard fjords and the Hausgarten observatory (Fram Strait) by combining diatom cultivation and 18S rRNA gene metabarcoding. In total, 50 diatom strains were isolated from the area during the HE492 cruise in August 2017. The strains were identified taxonomically using molecular and morphological approaches, and their biogeographic distribution was mapped using the local metabarcoding dataset and a global compilation of published metabarcoding datasets. Biogeographic analysis was also conducted for the locally most abundant diatom metabarcoding amplicon sequence variants. The biogeographic analyses demonstrated that Arctic diatoms exhibit three general biogeographic distribution types: Arctic, Arctic-temperate, and cosmopolitan. At Hausgarten and in outer Isfjorden on the west coast of Svalbard, the communities were dominated by genotypes with Arctic-temperate and cosmopolitan distribution. Diatom communities in nearby Van Mijenfjorden, inner Isfjorden and Kongsfjorden were dominated by genotypes with Arctic-temperate distribution, and cosmopolitan species were less abundant. The genotypes endemic to the Arctic had lower abundance on the west coast of Svalbard. The two northernmost fjords (Woodfjorden and Wijdefjorden) had a higher abundance of genotypes endemic to the Arctic. Our results demonstrate that the diatom communities in the Svalbard area consist of genotypes endemic to the Arctic, and genotypes with broader biogeographic distribution, all of which are further structured by local environmental gradients. Finer biogeographic patterns observed within Arctic-temperate and cosmopolitan genotypes suggest that certain genotypes can be used as indicators of increasing influence of Atlantic waters on the phytoplankton community structure in the Svalbard area.

Suspended particulate matter drives the spatial segregation of nitrogen turnover along the hyper-turbid Ems estuary

Abstract. Estuaries are nutrient filters and change riverine nutrient loads before they reach coastal oceans. Their morphology have been extensively changed by anthropogenic activities like draining, deepening and dredging to meet economic and social demand, causing significant regime changes like tidal amplifications and in some cases to hyper-turbid conditions. Furthermore, increased nutrient loads, especially nitrogen, mainly by agriculture cause coastal eutrophication. Estuaries can either act as a sink or as a source of nitrate, depending on environmental and geomorphological conditions. These factors vary along an estuary, and change nitrogen turnover in the system. Here, we investigate the factors controlling nitrogen turnover in the hyper-turbid Ems estuary (Northern Germany), which has been strongly impacted by human activities. During two research cruises in August 2014 and June 2020, we measured water column properties, dissolved inorganic nitrogen, dual stable isotopes of nitrate and dissolved nitrous oxide concentration along the estuary. We found that three distinct biogeochemical zones exist along the estuary. A strong fractionation (∼26 ‰) of nitrate stable isotopes points towards nitrate removal via water column denitrification in the hyper-turbid tidal river, driven by anoxic conditions in deeper water layers. In the middle reaches of the estuary nitrification gains importance, turning this section into a net nitrate source. The outer reaches are dominated by mixing, with nitrate uptake in 2020. We find that the overarching control on biogeochemical nitrogen cycling, zonation and nitrous oxide production in the Ems estuary is exerted by suspended particulate matter concentrations and the linked oxygen deficits.

Distribution of Coomassie Blue Stainable Particles in the Pearl River Estuary, China, Insight Into the Nitrogen Cycling in Estuarine System

Distributions of Coomassie Blue stainable particles (CSP), the sources and transports, as well as their implications for nitrogen biogeochemical cycles in the Pearl River estuary (PRE) were investigated during two cruises in August 2016 and January 2017. CSPcolor concentrations (CSP concentration determined spectrophotometrically) were 73.7–685.3 μg BSA eq L–1 [μg Bovine serum albumin (BSA) equivalent liter–1] in August 2016 and 100.6–396.4 μg BSA eq L–1 in January 2017, respectively. CSP concentrations were high in low-salinity waters (<5), and declined from the river to the middle estuary by 80% in the wet season and 55.6% in the dry season, respectively, then increased again in the lower estuary due to high primary production. CSP concentrations were mainly associated with chlorophyll a (Chl a) concentration except for the turbid mixing zone, suggesting that autochthonous phytoplankton production served as the primary source of CSP in the PRE. The concentrations of nitrogen (N) as CSP in the PRE were comparable to the nitrogen content of particulate hydrolysable amino acids (PHAA). Pictures of CSP taken by microscopy and the correlation between composition of PHAA and the ratio of Chl a/CSPcolor showed that CSP were relatively degraded due to delivery of old terrestrial protein to river section and extensive microbial degradation during mixing at the upper and middle parts of the estuary, whereas CSP in lower estuary appeared to be more labile due to higher fresh algal production. The contribution of CSP nitrogen (CSP-N) to the particulate nitrogen (PN) pool was 34.98% in summer and 30.8% in winter. The conservative estimate of CSP-N input flux in the Pearl River Delta was about 6 × 106 mol N d–1. These results suggested that CSP was a significant pool of organic nitrogen in the PRE. The study of CSP composition in terms of nitrogen provides new insight into the roles of CSP on nitrogen biogeochemical processes in the turbid and productive estuarine system.

Indirect estimation of turbulent mixing in western route of Indonesian throughflow

The Indonesian Throughflow (ITF) via its western path conveys mainly North Pacific water origin with Smax thermocline water and Smin intermediate water from its entry portal in Sangihe-Talaud arcs to the main outflow straits in Lombok, Ombai and Timor passage. Along its route, the throughflow water characteristics transforms significantly due to strong diapycnal mixing forced by internal tidal waves interaction along complex topography such as passages, sill, straits, and shallow islands chains. This paper reports a brief estimate of turbulent mixing profiles in Sangihe chains, and Makassar Strait. The CTD dataset are obtained from the year of maritime continent (YMC) Cruise in August 2019 on board the R.V. Baruna Jaya I. The Thorpe method is used to analysis dissipation energy ( ε ) and vertical diffusivity (Kz ) from CTD dataset. It is shown that the highest ε epsilon 5.87 × 10−7 Wkg −1 and Kz 4.42 × 10−3 m2s 1 are found in the Sangihe area. In Labani Channel and Dewakang Sill the averaged vertical diffusivity is much weaker at the order of 10−4 m 2s1. Thus, Sangihe Chains station have the highest values compared to other stations at depth 950-1000 meters.

Spatial distribution and source of biotoxins in phytoplankton from the South China Sea, China

Marine phycotoxins severely threaten ecosystem health and mariculture. This study investigates the spatial distribution and source of diverse phycotoxins in the South China Sea (SCS), during four 2019/2020 cruises. Saxitoxin (STX) and okadaic acid (OA) -groups, azaspiracids, cyclic imines, pectenotoxins (PTX), yessotoxins, and domoic acid (DA) toxins were analyzed in microalgal samples. PTX2 occurred with the highest (93.5%) detection rate (DR) during all cruises, especially in the Pearl River Estuary (PRE) in June 2019. Homo-yessotoxin (hYTX) and DA were found during three cruises in August 2020, and high DR of hYTX (67.7%, 29.3%) and DA (29.0%, 29.3%) in the PRE and Guangdong coast, respectively, in June 2019 and 2020, peaking at concentrations of 777 pg hYTX L-1 and 38514 pg DA L-1. The phycotoxin distribution demonstrated that DA-producing microalgae gathered close to the PRE and Guangdong coast, while hYTX-producing microalgae distributed relatively far offshore. Microalgae producing PTX2- and STX-group toxins were more widely living in the SCS. High-throughput sequencing results suggested that Alexandrium pacificum and Gonyaulax spinifera were responsible for STX-group toxins and hYTX, respectively, while Pseudo-nitzschia cuspidata was the main source of DA. Widely distributed PTX2, hYTX, and DA were reported for the first time in the SCS.

Distribution of Nutrient Salts and Chlorophyll-a in Surface Water along the Gulf of Aden and Arabian Sea Coast, Yemen

The distribution of inorganic nutrient salts (ammonium, nitrite, nitrate, phosphate and silicate) and phytoplankton chlorophyll-a (Chl-a) were investigated in the Gulf of Aden and along the Arabian Sea coast, Yemen. Seventy two surface water samples were collected during cruises in August 2014 and January 2015. The sampled area extends from As Suqayyah in the west of the Gulf of Aden to Hawf in the east. The study showed that the average values of nutrient salts (μg/l) in waters collected in August 2014 and January 2015 were 0.83 and 0.60, 10.98 and 10.03, 16.41 and 14.73, 10.36 and 8.76, and 29.72 and 22.67 for ammonium, nitrite, nitrate, phosphate and silicate, respectively. The average values of Chl-a (mg/m3) in August 2014 and January 2015 were 0.26 and 0.21, respectively. The results showed that nitrate levels were very high but those of ammonium very low. This may reflect the oxidation of ammonia to nitrite and then nitrate, leading to a very favorable ratio of ammonia and nitrate values. The low levels of dissolved inorganic nitrogen (DIN)compounds, (ammonium, nitrite, and nitrate), phosphate, silicate and Chl-a indicated that the southern coast of Yemen is not in a eutrophic condition. The highest nutrient salt values were in the eastern part of the study area, and may have resulted from water originating from the Indian Ocean and upwelling. Statistical analysis to seek correlations between nutrient salts and Chl-a show very good to excellent correlation, which may be due toconstant coastal environment.

Turbulent mixing of water masses in Selayar Slope - Southern Makassar Strait

During the Java-Makassar-Flores (JMF) Cruise in August 2015 onboard the RV GEOMARIN 3, about 56 hydrographic CTD profiles have been acquired in the JMF confluence region of Makassar Indonesian Throughflow (ITF) and seasonal Java-Flores Current, where three intensive 24-h CTD “yoyo” measurement were carried out in Dewakang sill, Selayar slope and Kangean slope. The aim of this paper is to estimates the turbulent mixing value using Thorpe analysis, particularly in the Selayar slope - southern Makassar Strait. It is found that below surface potential density of 24.25 σθ stratification of water masses is dominated by Makassar ITF with salty (34.6 psu) North Pacific thermocline water (NPSW) and less salty (34.42 psu) North Pacific lower-thermocline water (NPIW). However, above 24.25 σθ upper NPSW is drastically dominated by much fresher water (34.17 psu) from Java Sea, where inter-leavings of salinity profiles are obviously seen between surface density of 22.0 σθ and 23.50 σθ. Estimation of turbulent vertical eddy diffusivity in this upper-thermocline are found strong with the order of 10−3 m2/s. It is suggested that energetic internal tidal waves may contribute significantly to the vertical mixing in this complex topographic region.

An advanced sensor platform for acoustic quantification of the ocean twilight zone

The ocean twilight zone (OTZ) is the vast, globe-spanning, layer of water between 200 and 1000 m depth—home to diverse communities of small fishes, cephalopods, crustaceans, and gelatinous organisms. Yet, little is known about the biology, abundance, biomass, and distribution of these organisms. The OTZ is difficult to sample due to a combination of organism patchiness and avoidance, and difficulties capturing fragile species. Recent evidence suggests that the global OTZ biomass may be sufficient to commercially harvest. Furthermore, much of this biomass performs daily vertical migration (DVM) and may play a potentially critical role in regulating Earth’s climate through the export of carbon to the deep ocean. Deep-See, an advanced senor platform, was developed to fill the technological void for characterizing the OTZ. This towed vehicle integrates wide-band, split-beam acoustics (1–500 kHz) with optical, environmental, and eDNA sensors that can address many of the challenges associated with sampling in the OTZ. Data from the inaugural cruise in August 2018 highlight that (i) a surprisingly high abundance of organisms can be found outside the dense sound scattering layers and (ii) the target strength of many organisms that perform DVM changes with the depth, which is critical to estimate biomass.

Presence of high nitrate to phosphate ratio subsurface water in the Tsushima Strait during summer

To identify water with an excess nitrate concentration to phosphate ratio and its potential source, the nutrient concentrations in the Tsushima Strait (TSS) were investigated over ten cruises in August and September 2007–2014, excluding 2010. On the basis of the Redfield ratio, water with an excess nitrate concentration of >1 μM (positive ExNOx water) was identified below the surface mixed layer during four cruises in 2011–2013. Positive ExNOx water was present mainly in less-saline (<34) waters with a density of 22–25 σ θ , and 25–75 m depth. However, in August 2012, positive ExNOx was detected in dense (25–25.5 σ θ ) and deep (50–110 m depth) waters near the salinity maximum, although the salinity during this period was significantly lower than that in other years. The horizontal length of positive ExNOx water was >100 km across the TSS during two cruises in August 2012 and September 2013, respectively. According to multi-regression analysis conducted on the silicate concentration, temperature, and salinity, the silicate concentration was increased in the less-saline subsurface water. The required amount of original freshwater was 108–9 m3 day−1 based on the excess nitrate concentration. This evidence indicates that the positive ExNOx water originated from large river waters such as the Changjiang. Thus, discharged water from the rivers of the East Asia is contributing to the increased N:P ratio in the Tsushima Warm Current, southern Japan Sea.

Analysis of turbulent mixing in Dewakang Sill, Southern Makassar Strait

Dewakang Sill is located in southern Makassar Strait, conveying major path of Indonesian Throughflow (ITF), as a confluence region of different water masses, such as salty Pacific water and fresh Java Sea water. Its depth is about 680 m which blocks the ITF flow below this depth into Flores Sea. This research aimed to estimate turbulent mixing in the Dewakang Sill by applying Thorpe analysis using 24 hours “yoyo” CTD data sets, acquired from MAJAFLOX Cruise in August 2015. The results showed that stratification of water masses is dominated by Pacific water origin. Those are North Pacific Subtropical thermocline and Intermediate water masses. Mean dissipation of turbulent kinetic energy (ϵ) and turbulent vertical diffusivity (Kρ) value in the Dewakang Sill are of O(1.08 × 10−6)Wkg−1, and O(2.84 × 10−4) m2s−1 respectively. High correlation between epsilon and internal waves oscillation suggested that internal tidal waves activities are the major forcing for turbulent mixing in the study area.

Geology and biology of the “Sticky Grounds”, shelf-margin carbonate mounds, and mesophotic ecosystem in the eastern Gulf of Mexico

Shelf-margin carbonate mounds in water depths of 116–135m in the eastern Gulf of Mexico along the central west Florida shelf were investigated using swath bathymetry, side-scan sonar, sub-bottom imaging, rock dredging, and submersible dives. These enigmatic structures, known to fisherman as the “Sticky Grounds”, trend along slope, are 5–15m in relief with base diameters of 5–30m, and suggest widespread potential for mesophotic reef habitat along the west Florida outer continental shelf. Possible origins are sea-level lowstand coral patch reefs, oyster reefs, or perhaps more recent post-lowstand biohermal development. Rock dredging recovered bioeroded carbonate-rock facies comprised of bored and cemented bioclastics. Rock sample components included calcified worm tubes, pelagic sediment, and oysters normally restricted to brackish nearshore areas. Several reef sites were surveyed at the Sticky Grounds during a cruise in August 2010 with the R/V Seward Johnson using the Johnson-Sea-Link II submersible to ground truth the swath-sonar maps and to quantify and characterize the benthic habitats, benthic macrofauna, fish populations, and coral/sponge cover. This study characterizes for the first time this mesophotic reef ecosystem and associated fish populations, and analyzes the interrelationships of the fish assemblages, benthic habitats and invertebrate biota. These highly eroded rock mounds provide extensive hard-bottom habitat for reef invertebrate species as well as essential fish habitat for reef fish and commercially/recreationally important fish species. The extent and significance of associated living resources with these bottom types is particularly important in light of the 2010 Deepwater Horizon oil spill in the northeastern Gulf and the proximity of the Loop Current. Mapping the distribution of these mesophotic-depth ecosystems is important for quantifying essential fish habitat and describing benthic resources. These activities can improve ecosystem management and planning of future oil and gas activities in this outer continental shelf region.

Rosetta Lander – Landing and operations on comet 67P/Churyumov–Gerasimenko

The Rosetta Lander Philae is part of the ESA Rosetta Mission which reached comet 67P/Churyumov–Gerasimenko after a 10 year cruise in August 2014. Since then, Rosetta has been studying both its nucleus and coma with instruments aboard the Orbiter. On November 12th, 2014 the Lander, Philae, was successfully delivered to the surface of the comet and operated for approximately 64h after separation from the mother spacecraft. Since the active cold gas system aboard the Lander as well as the anchoring harpoons did not work, Philae bounced after the first touch-down at the planned landing site “Agilkia”. At the final landing site, “Abydos”, a modified First Scientific Sequence was performed. Due to the unexpectedly low illumination conditions and a lack of anchoring the sequence had to be adapted in order to minimize risk and maximize the scientific output. All ten instruments could be activated at least once, before Philae went into hibernation. In June 2015, the Lander contacted Rosetta again having survived successfully a long hibernation phase.This paper describes the Lander operations around separation, during descent and on the surface of the comet. We also address the partly successful attempts to re-establish contact with the Lander in June/July, when the internal temperature & power received were sufficient for Philae to become active again.

Real-time redox speciation of iron in estuarine and coastal surface waters.

An automated, shipboard-use system was developed for real-time speciation of iron in coastal surface waters. It comprised a towed Fish underway sampler and a modified reverse flow injection analysis system with a liquid waveguide capillary flow cell-spectrophotometric detection device. The detection was based on the reaction between ferrozine and Fe(II). The detection limits of 0.3 and 0.7 nM were achieved for Fe(II) and Fe(II+III), together with their respective dynamic linear ranges of 0.5-250 and 0.9-250 nM. The system was successfully deployed and run consecutively for about 1 week during a cruise in August 2009 to the East China Sea off the Changjiang Estuary. The distribution of operationally defined field dissolvable Fe(II) and Fe(II+III) (expressed as Fea(II) and Fea(II+III)) in these areas was obtained, which showed that both Fea(II) and Fea(II+III) concentrations decreased with salinity when there were relatively high Fea(II) concentrations (up to about 120 nM) near shore. A distinct distribution of Fea(II) to Fea(II+III) ratios was also revealed, with a ratio of 0.58 in the water off Changjiang Estuary and 0.19 in the open ocean.

Seasonal and spatial patterns of picophytoplankton growth, grazing and distribution in the East China Sea

Abstract. Dynamics of picophytoplankton population distribution in the East China Sea (ECS), a marginal sea in the western North Pacific Ocean, were studied during two cruises in August 2009 (summer) and January 2010 (winter). Dilution experiments were conducted during the two cruises to investigate the growth and grazing among picophytoplantkon populations. Comparisons of phytoplankton growth (μ0) and microzooplankton grazing rates (m) on seasonal (summer and winter), spatial (plume, transitional and Kuroshio regions) and vertical (surface and depth of chlorophyll maximum) scales were made. The three picophytoplankton populations occupied different ecological niches and showed different distribution patterns (especially in summer), which is, however, not coincident with their maximum growth rate. The distribution and population transition of picophytoplankton is therefore a result of the balance between growth and grazing mortality. Average growth rates (μ0) for Prochlorococcus (Pro), Synechococcus (Syn) and picoeukaryotes (Peuk) were 0.36, 0.81 and 0.90 d−1 in summer, and 0.46, 0.58 and 0.56 d−1 in winter, respectively. Average grazing mortality rates (m) were 0.46, 0.63 and 0.68 d−1 in summer, and 0.25, 0.22 and 0.23 d−1 in winter for Pro, Syn and Peuk, respectively. The spatial pattern of both growth and grazing mortality rates showed decreasing trends from the inshore to offshore region, indicating a strong influence of the nutrient gradient induced by Yangtze River input. In summer, Pro, Syn and Peuk were dominant in Kuroshio, transitional and plume regions, respectively, while in winter all the three populations tended to thrive in the offshore regions, particularly for Pro and Syn. Vertically, picophytoplankton exhibited the highest abundance at ~ 20 m in summer and at the surface in winter. Both growth rate and grazing mortality were higher at the surface than in the deep chlorophyll maximum (DCM) layer. On average, protist grazing consumed 84, 79 and 74% and 45, 47 and 57% of production for Pro, Syn and Peuk in summer and winter, respectively.

Heat Flow and Fluid Flux in Cascadia's Seismogenic Zone

The Cascadia Subduction Zone in the northeast Pacific has generated a large number of megathrust earthquakes and poses the greatest recognized seismic hazard to the northwestern United States. To learn more about the risks this subduction zone poses, scientists from Washington and Oregon conducted a research cruise in August aboard the R/V Atlantis to collect data that would estimate the thermal structure of the “fully locked zone” of the Cascadia megathrust fault. This locked zone resists the continuous motion of two tectonic plates as they collide, storing compressional energy that produces large megathrust earthquakes when released by the abrupt failure of the locking process.

Inorganic carbon parameters responding to summer hypoxia outside the Changjiang Estuary and the related implications

The eutrophication, hypoxia and coastal acidification are attracting more and more attention. In this study, inorganic carbon parameters, including dissolved inorganic carbon (DIC), total alkalinity (TA) and calculated partial pressure of CO2 (pCO(2)), obtained from a summer cruise in August, 2009, were used to investigate their integrated response to biological processes accompanying the oxygen depletion in the areas off the Changjiang Estuary. According to the observations, the typical hypoxia occurred in the bottom water just outside the Changjiang Estuary with Dissolved Oxygen (DO) lower than 2.00 mg L-1. The biological uptake in the surface water and the decomposition of organic matter in the bottom water were fully coupled with each other. The high concentration of Chl_a (Chl_a = 10.9 mu g L-1) and DO (9.25 mg L-1), profoundly decreased DIC concentration (1828 mu mol kg(-1)) and elevated pH (8.42) was observed in the surface water. The correspondingly increased DIC and depletion of oxygen were observed in the bottom water. The semi-quantitative analysis proved that the locally-produced phytoplankton, determined by primary productivity, was deposited to the bottom and contributed about 76% of total amount of the organic carbon decomposition in the bottom. However, in the bottom hypoxia (DO = 2.05 mg L-1) area observed in the Southern Zhejiang coastal water, the responding patterns of inorganic carbon parameters deviated from the previous one. The expanding of Changjiang Diluted Water (CDW), the adding of Hangzhou Bay water (with high DIC concentration) and Coastal Current together modify the DIC background value in this area, and the local degeneration and upwelling process may also help to offset the local DIC removed by net biological uptake in surface water. In addition, when the mixing occurring in autumn, which may break the summer stratification, the excess release of high DIC in the bottom water to the subsurface water could have an important influence on coastal acidification and the CO2 uptake capacity in this area.

Toxicity and Mutagenicity of Gulf of Mexico Waters During and After the Deepwater Horizon Oil Spill

The Deepwater Horizon oil spill is unparalleled among environmental hydrocarbon releases, because of the tremendous volume of oil, the additional contamination by dispersant, and the oceanic depth at which this release occurred. Here, we present data on general toxicity and mutagenicity of upper water column waters and, to a lesser degree, sediment porewater of the Northeastern Gulf of Mexico (NEGOM) and west Florida shelf (WFS) at the time of the Deepwater Horizon oil spill in 2010 and thereafter. During a research cruise in August 2010, analysis of water collected in the NEGOM indicated that samples of 3 of 14 (21%) stations were toxic to bacteria based on the Microtox assay, 4 of 13 (34%) were toxic to phytoplankton via the QwikLite assay, and 6 of 14 (43%) showed DNA damaging activity using the λ-Microscreen Prophage induction assay. The Microtox and Microscreen assays indicated that the degree of toxicity was correlated to total petroleum hydrocarbon concentration. Long-term monitoring of stations on the NEGOM and the WFS was undertaken by 8 and 6 cruises to these areas, respectively. Microtox toxicity was nearly totally absent by December 2010 in the Northeastern Gulf of Mexico (3 of 8 cruises with one positive station). In contrast, QwikLite toxicity assay yielded positives at each cruise, often at multiple stations or depths, indicating the greater sensitivity of the QwikLite assay to environmental factors. The Microscreen mutagenicity assays indicated that certain water column samples overlying the WFS were mutagenic at least 1.5 years after capping the Macondo well. Similarly, sediment porewater samples taken from 1000, 1200, and 1400 m from the slope off the WFS in June 2011 were also highly genotoxic. Our observations are consistent with a portion of the dispersed oil from the Macondo well area advecting to the southeast and upwelling onto the WFS, although other explanations exist. Organisms in contact with these waters might experience DNA damage that could lead to mutation and heritable alterations to the community pangenome. Such mutagenic interactions might not become apparent in higher organisms for years.