Crude Nutrient Content Research Articles

Estimation of some Nutrient Values of Quinoa, Chia, Teff, Mung Bean and Buckwheat Seeds for Ruminants by In Vitro Methods

Objective: The aim of this study was to investigate the availability of quinoa, chia, teff, mung bean, and buckwheat as an alternative feed in ruminant nutrition.Material and Methods: The materials were composed of quinoa (white, black, red), chia (white, black), teff, mung beans and buckwheat, which have passed their shelf life due to tear or damage in their package. Fatty acid components as well as enzyme soluble organic matter (ESOM) under in vitro conditions were also determined in addition to crude nutrient contents. Metabolic energy (ME), using the obtained data, was also calculated.Results: It was found out that the crude protein (CP) contents were 14.04-15.38% in quinoa, 19.90-21.73% in chia, 11.98% in teff, 25.20% in mung bean, and 13.72% in buckwheat. Ether extract (EE) contents; however, were found out to be 7.13-7.97%, 30.84-35.75%, 2.46, % 3.48%, and 5.25%. The highest amount of linoleic acid was found in chia (60.71-66.17%). ESOM contents was determined at high level in white quinoa, mung bean, and buckwheat. However, in vitro ME contents were found at the highest level in chia.Conclusion: Quinoa has the potential to be an alternative to traditional cereals during early lactation period in ruminant animals, in which energy need is at its highest level, or as a source of energy in fattening period. Chia has a high potential to be effective in preventing heat stress, especially in dairy cattle. The high linolenic acid content of chia oil may allow the milk fat to be enriched by conjugated linoleic acid (CLA), one of the polyunsaturated fatty acids. It has been determined that teff and buckwheat can be alternatives to other cereals, whereas mung bean, due to its crude protein being high and being rich in minerals is a significant alternative for feed producers who have been in search of new sources of protein recently.

Spraying opened sugar beet pulp silage with oregano essential oil helps to sustain quality and stability

This study was conducted to determine the effects of spraying oregano essential oil (OEO) onto sugar beet pulp silage (SBPS) on silage quality and aerobic stability after opening. A factorial experiment with three replicates of three treatments and four time periods was conducted using laboratory-type plastic silos. The treatments were an untreated control, silage sprayed with 10 ml/75 cm2 OEO, and silage sprayed with 20 ml/75 cm2 OEO. The silages were sampled at 0, 72, 120, and 168 hours after spraying. Temperature, L*, a*, and b* colour values, pH, water-soluble carbohydrates, crude nutrient contents, Fleig score, metabolizable energy (ME) value, lactic acid bacteria (LAB), total live bacteria (TLB), yeast, and mould formation were assessed. Spraying OEO onto SBPS did not affect L*, a*, and b* values, pH, water-soluble carbohydrates, and Fleig score values, but decreased temperature. Spraying OEO onto the silage increased organic matter, ether extract, acid detergent fibre, neutral detergent fibre and acid detergent lignin contents without affecting crude protein, crude fibre, nitrogen free extract, and ME contents. Irrespective of treatment, crude protein, ether extract, acid detergent fibre, acid detergent lignin, nitrogen free extract, Fleig score, and ME contents of silages increased with time after spraying. The OEO spraying reduced LAB, TLB, and yeast contents in silages. In conclusion, OEO spraying onto opened SBPS reduced LAB, TLB, and yeast formation and stopped mould growth up to 72 hours without affecting their nutritional properties, suggesting that OEO could be used to ensure the stability of SBPS.
 Keywords: aerobic stability, mould growth, silage microbiology, nutrients, feeding management

Physical Properties, Crude Nutrient Content, and Nutritive Values of Fish Meals Produced from Overflowed Fishes for Laying Quails

Physical Properties, Crude Nutrient Content, and Nutritive Values of Fish Meals Produced from Overflowed Fishes for Laying Quails

High fibre selection by roe deer (Capreolus capreolus): evidence of ruminal microbiome adaption to seasonal and geographical differences in nutrient composition

Context. The European roe deer owes its ability to digest fibre to its microbiome. This is made up of many different species at different levels of abundance and with different differentiations. In Europe, the roe deer is often classified as a so-called ‘concentrate selector’. This term has often been interpreted by different researchers to mean a selector of either protein- or energy-rich food. According to various studies, this selection behaviour is due to the low abundance of fibre-degrading microorganisms. Aims. The aim of the present study was to determine the concentration of crude nutrients in the rumen of roe deer, with the focus on the fibre fractions, and to show changes among seasons and between habitats. Furthermore, the aim was to find out how far the composition of the ruminal microbiota adapts to these changes. Methods. From 2011 to 2014, we collected the rumens of 245 roe deer in two Bavarian habitat types, a forest and an agricultural habitat. The crude nutrient contents and the size of the total microbiome and the proportions of individual genera were determined in the rumen content. Key results. The average annual concentration of crude fibre in the ingested food is 26–30% and this rises to 38% in certain months. The forest roe deer had the highest proportions of crude fibre in their food and the concentrations of other nutrients were also highly dependent on the season and habitat. Furthermore, the animals also have far less protein in their rumen content than often assumed. The total number of microorganisms in the rumens of the forest deer is significantly higher than in animals living in the agricultural area. The number of microorganisms was highest in the forest roe deer in winter, and in the roe deer from the agricultural area in summer. Clear connections can also be seen between individual groups of microorganisms and particular crude nutrients. The high crude-fibre concentration leads to a high number of fibre-degrading microorganisms, such as, for example, anaerobic fungi or the Ruminococcus flavefaciens. Conclusions. The results showed a high adaptability of the animals to a fibre-rich diet. The microbiome adapts very well to the respective nutrient availabilities. This, in turn, is what allows the roe deer to adapt so readily to diverse habitats and environmental conditions. Implications. Due to the generally high concentrations of fibre, combined with the high numbers of fibre-degraders in the rumen, we suggest that, from now on, we should talk of a roe deer as being a ‘selector’ or ‘browser’ rather than a ‘concentrate selector’.

Effect of Gamma Irradiation on Microbial Decontamination, Crude Nutrient Content, and Mineral Nutrient Composition of Laboratory Animal Diets.

Laboratory animal models are an important part of test design. Certain conditions such as microbial contamination in diets of these models could affect the results of experiments. One of the most important routes that predispose to contamination is generated through feeding of laboratory animals. This study aimed to show the effect of gamma irradiation in reducing bacteria concentrations, crude nutrient content, and concentrations of some minerals and trace elements in laboratory animal diets. Large-sized pellets with 10–15 mm diameter (commonly used for rats and hamsters) and small-sized pellets with 3–5 mm diameter (used for rabbits and guinea pigs) along with skimmed milk powder (SMP) as a food additive were exposed to gamma irradiation with different doses ranging from 3 to 30 kGy. The total microbial contamination and any possible changes in some mineral nutrient composition and the crude nutrient content were determined pre- and post-irradiation. Our data revealed that 25 kGy in pelleted diets and 18 kGy in SKM had superior effects in the reduction of bacterial contamination with little change in crude nutrient content and minerals and trace elements in nutrient requirements of laboratory animals. According to the results, gamma irradiation had minimal effects on crude nutrient content and the concentrations of some minerals and trace elements of laboratory animal diets, and it also eliminated bacterial and fungal contamination load. By using gamma irradiation, this method could yield a favorable outcome in controlling microbial contamination of animal diets.

Determination of polyphenol and crude nutrient content and nutrient digestibility of dried and ensiled white and red grape pomace cultivars

The present study aimed to determine the nutrient and energy content of fresh and ensiled grape pomace (GP) from different grape varieties originating from Germany, and to estimate the feed value of dried white, dried red and ensiled white GP by calculating nutrient digestibility and the content of metabolisable energy (ME) and net energy lactation (NEL) measured in sheep as a ruminant model. GP from red cultivars had higher contents of organic matter (OM), crude protein (CP), ether extract (EE), crude fibre (CF), total phenolic contents (TPC) and ME, whereas the concentrations of ash and sugar were lower than from white cultivars. Compared with untreated GP, ensiled GP had increased concentrations of CP (+19%), ether extract (EE; +23%) and CF (+12%) and a higher ME content (+7%) and markedly decreased concentrations of sugar (−99.6%) and TPC (−48%). The concentrations of dry matter, OM and ash were not different between ensiled and fresh GP. Compared with dried GP, ensiled GP had a higher nutrient digestibility (OM, +32%; CP, +43%; CF, +46%; neutral detergent fibre [NDF], +54%; acid detergent fibre [ADF], +69%) and higher energy values (ME, +16%; NEL, +19%). The digestibility of OM, CP, EE and CF and the energy content were higher for dried red than for dried white GP, whereas the digestibility of NDFOM and ADFOM was lower for dried red than dried white GP. In conclusion, the results show that both red and white GP are suitable dietary sources for enrichment with TPC. Furthermore, compared with drying ensiling of GP improves the feeding value of GP and is a good possibility of preserving the seasonally produced by-product of winemaking for ruminant feeding.

Effects of free air carbon dioxide enrichment and drought stress on the feed value of maize silage fed to sheep at different thermal regimes

Information about the effects of rising atmospheric CO2 concentration and drought on the feed value of maize silage and interactions with the thermal environment during feeding is limited. A free air carbon dioxide enrichment facility was operated in a maize field to generate an elevated CO2 concentration of 550 ppm. Drought was induced by the exclusion of precipitation in one half of all experimental plots. Plants were harvested, chopped and ensiled. In a balance experiment on sheep, the nutrient digestibility was determined for three climatic treatments (temperate, temperature humidity index (THI) 57–63; mild heat, THI 68–71; severe heat, THI 75–80). The CO2 concentration and drought did not alter the crude nutrient content of silage dry matter (DM) or nutrient and organic matter (OM) digestibility. Drought increased the concentration of deoxynivalenol (DON, p < 0.001). The drought-associated increase of DON was reduced by CO2 enrichment (p = 0.003). The lowest digestibility of acid detergent fibre (p = 0.024) and neutral detergent fibre (p = 0.005) was observed during the coldest climate. OM digestibility increased during mild heat (p = 0.023). This study did not indicate considerable alterations of the feed value of maize silage due to increased atmospheric CO2 and drought. Enriched CO2 may decrease DON contaminations during drought. The thermal environment during the balance experiment did not interact with feeding maize silage grown under elevated CO2, but may affect cell wall and OM digestibility.

Genetically modified feeds in animal nutrition 2nd communication: Glufosinate tolerant sugar beets (roots and silage) and maize grains for ruminants and pigs

To analyse substantial equivalence of genetically modified sugar‐beets and maize, in which the glufosinate‐tolerant (Pat) gene is inserted, crude nutrients, the amino acid and the fatty acid profiles as well as the composition of the NDF‐fraction of maize grains were determined and compared with those of the corresponding non‐transgenic cultivars. Due to the genetic manipulation differences in crude nutrient contents including sugar and starch were not detected. The amino acid profile of maize grains was analysed to be the same. Fatty acid profile and composition of cell wall constituents did not show any influences as well. Digestibility of Pat‐sugar‐beets and maize grains for pigs did not demonstrate meaningful differences as compared to the corresponding non‐transgenic cultivars. Digestibility of sugar‐beet roots and sugar‐beet top silage for ruminants proved to be also in the scope of natural variance. As the digestibility of the macro nutrients remained unaffected, the Pat‐gene introduction into both crops did not show an influence on the energetic feeding value. For pigs the ME‐content of Pat‐sugar‐beets was determined to be 14.1 MJ/kg DM versus 13.7 MJ of the non‐transgenic cultivars. ME‐content of Pat‐maize grains was 16.0 MJ/kg DM versus 15.8 MJ for controls. For ruminants the feeding value of Pat‐sugar‐beets was found to be 8.5 MJ NEL/kg DM or 13.2 MJ ME/kg DM, regardless of whether the Pat‐gene was inserted or not. The corresponding energy values of sugar‐beet top silage ranged between 5.2 and 5.5 MJ NEL/kg DM or 8.6 and 9.1 MJ ME/kg DM, with differences considered in the biological range.

Composition and digestibility of untreated and chemically treated animal excreta for ruminants—A review

The paper reports on the crude nutrient content and the mineral profile, as well as the apparent digestibility by ruminants, of faeces of poultry, pigs and cattle, poultry litter and solid matter of poultry, pig and cattle slurries. The second part of the paper deals with chemical treatment of pig and cattle slurry solids with NaOH, KOH and urea. Nutrient and mineral content as well as digestibility of animal excreta are greatly influenced by species, age and type of feeding, the bedding material used and the method of solid-liquid separation of slurries. The digestibility of poultry excreta is higher than that of pig waste. Cattle faeces are unsuitable as feeds even after chemical treatment. In sacco degradability, apparent digestibility, energy content and intake of pig slurry solids were increased by treatment with urea, NaOH and KOH. Level of chemicals used and temperature influenced the effect of treatment. Wet pig-slurry solids may be preserved with urea.

Prediction of metabolizable energy for pigs on the basis of crude nutrients in the feeds

A number of correlation and regression analyses were performed on data from digestibility and N-balance experiments with growing pigs (carried out during the years 1966–1982), in order to investigate whether the metabolizable energy content of pig feeds could be estimated with a reasonable degree of accuracy on the basis of their crude nutrient content (chemical analysis). The data comprise 321 diets varying widely in chemical composition and 331 feedstuff samples including 92 different feedstuff sources. Three or more proximate analyses and usually five or more digestibility and N-balance experiments were conducted with each feed sample. The statistical analyses were performed on average values of proximate analyses and average metabolizable energy values from the repeated digestibility and N-balance experiments. Crude fibre accounted for 74 and 62% of the variation in the digestability of gross energy in diets and feedstuffs, respectively. Stepwise regression analyses showed that metabolizable energy in the diets could be estimated on basis of crude fibre and gross energy with R 2 = 0.75 and CV = 5.2. The best prediction ( R 2 = 0.77 CV = 5.0) was obtained including crude protein, crude fibre, NFE substances and organic matter. A common regression analysis with the intercept fixed at zero showed that metabolizable energy could be estimated on the basis of crude protein, crude fat, crude fibre and NFE substances with R 2 = 0.77 and CV = 5.0. Estimation of metabolizable energy in the individual feedstuffs on basis of crude fibre and organic matter was much poorer ( R 2 = 0.73, CV = 10.5). The best prediction ( R 2 = 0.87, CV = 7.2) was obtained by including crude protein, crude fibre, NFE substances, NDF, gross energy and organic matter. Deleting 31 feedstuff samples of animal origin improved the accuracy of the prediction equation ( R 2 = 0.86, CV = 6.6).

The substitution of protein feed by lysine-supplemented protein-rich wheat during the raisung and laying periods in hens. 6. Report. The effect of graded lysine supplemented on the crude nutritional content of the carcas of laying hens.

In the present work 8 hens taken from each of the 16 experimental groups (90 birds per group) were killed at the end of the trial period (52 weeks). The weight of the organs was determined and bones, the utilizable parts and the residual carcass were analyzed for their crude nutrient content. The experimental birds received rations containing a large proportion of high-protein wheat supplemented with varying levels of lysine. Variations in the lysine supply did not affect the mass of blood, feathers, bones, liver, stomach, heart and ovaries, including ovarian follicles. An analysis of the utilizable parts (flesh, stomach, heart, liver, follicles, fat) for crude nutrients showed that the heavier birds receiving adequate amounts of lysine contained less crude protein and more crude fat than the smaller birds. A positive correlation was found to exist between the crude ash content of these samples (expressed as %) and the levels of lysine supplied during the laying period. All the birds receiving the lysine-deficient ration during the time of rearing or during the laying period contained significantly less crude ash in their bones. Alongside with the crude ash content the phosphorus content of the bones decreased when the birds where fed the diet for laying hens.

Die Substitution von Eiweißfuttermitteln durch lysinergänzten proteinreichen Weizen während der Aufzucht und Legeperiode von Hennen

A large-scale trial was carried out under commercial conditions to investigate to which extent the feeding of rations with varying lysine content to young hens would influence the crude nutrient content of the body fractions and might change the pattern of amino acid composition and the activity of GOT in the blood. Four rations were fed each containing 14.2% crude protein, 626-633 EFh units/kg and 0.59%, 0.61%, 0.54% and 0.46% lysine. The crude protein and crude fat content of the total body and of body fractions were in no case found to be related to the feeding regime. The proportion of crude ash in the "remainder of non-utilizable parts", in "bones", "intestinal and abdominal fat", "ovaries" and "small intestine" decreased with the decreasing lysine content of the rations. The concentrations of free lysine, histidine, arginine, and phenylalanine in the deproteinized blood plasma of the young hens were significantly (a = 0.01) lower in the birds of the lysine deficient group than in the hens of the other groups. Positive regressions were calculated for the lysine content or the content of aspartic acid in the ration and the pattern of free amino acids in blood plasma. Positive and negative linear relationships were found to exist between the concentrations of free lysine, and those of histidine, arginine or threonine, and serine. A close correlation existed between the total amount of essential amino acids in blood plasma and the lysine concentrations of the plasma. Increasing lysine supplementation produced a decline in the N content of the whole blood and blood corpuscles but a rise in the N content of blood plasma. The activity of GOT in the blood of young hens was not found to be useful as an indicator of the quality of dietary proteins.