Cross-site Comparison Research Articles

Ecosystem Structure and Function across Western Dryland Ecosystems: A Cross-Site Comparison of Semiarid Ecosystem Types in Colorado and Wyoming

Ecosystem Structure and Function across Western Dryland Ecosystems: A Cross-Site Comparison of Semiarid Ecosystem Types in Colorado and Wyoming

Using OPM-MEG in contrasting magnetic environments

Magnetoencephalography (MEG) has been revolutionised by optically pumped magnetometers (OPMs). “OPM-MEG” offers higher sensitivity, better spatial resolution, and lower cost than conventional instrumentation based on superconducting quantum interference devices (SQUIDs). Moreover, because OPMs are small, lightweight, and portable they offer the possibility of lifespan compliance and (with control of background field) motion robustness, dramatically expanding the range of MEG applications. However, OPM-MEG remains nascent technology; it places stringent requirements on magnetic shielding, and whilst a number of viable systems exist, most are custom made and there have been no cross-site investigations showing the reliability of data. In this paper, we undertake the first cross-site OPM-MEG comparison, using near identical commercial systems scanning the same participant. The two sites are deliberately contrasting, with different magnetic environments: a “green field” campus university site with an OPM-optimised shielded room (low interference) and a city centre hospital site with a “standard” (non-optimised) MSR (higher interference). We show that despite a 20-fold difference in background field, and a 30-fold difference in low frequency interference, using dynamic field control and software-based suppression of interference we can generate comparable noise floors at both sites. In human data recorded during a visuo-motor task and a face processing paradigm, we were able to generate similar data, with source localisation showing that brain regions could be pinpointed with just ∼10 mm spatial discrepancy and temporal correlations of > 80%. Overall, our study demonstrates that, with appropriate field control, OPM-MEG systems can be sited even in city centre hospital locations. The methods presented pave the way for wider deployment of OPM-MEG.

Hydrogeomorphic Differences between Proximate Rivers Affect Use by Large Predatory Fishes

Resource managers can benefit from understanding how hydrology, geomorphology, and vegetation type affect fish populations and expectations of fishing quality at varying spatiotemporal scales. We used electrofishing to examine abundance and distribution of predatory fishes in 2 proximate rivers in southwest Florida for 4 years. We conducted a cross-site comparison to quantify relationships between habitat (e.g., geomorphological features, shore types, in-water structure), water conditions, and abundances of large predatory fishes. We found abundances of Centropomus undecimalis (Common Snook; euryhaline species) and Lepisosteus platyrhinchus (Florida Gar; obligate air breather) were comparable between the Peace and Myakka rivers, and low abundance of Micropterus floridanus (Florida Bass) was associated with low dissolved oxygen in the Myakka River. The lowest dissolved oxygen occurred just downstream of large in-stream marshes, where conditions become hypoxic following seasonal vegetation die-offs. Understanding how habitat availability and water conditions favor fishes within a waterbody will guide how interventions to improve fishing quality (e.g., stocking fish) can be successful or if angler expectations need to be adjusted.

A cross-site comparison of online review manipulation using Benford’s law

There is a growing concern that online reviews are targets of systematic manipulation, and manipulated reviews serve purposes other than informing consumers. In this article, we report a cross-site comparison of the aggregate-level manipulation using Benford’s law to detect anomalies. Benford’s law states that digits in naturally occurring data follow a logarithmic distribution. Deviation from such distribution is considered as a sign of systematic manipulation. We empirically examine word-count distributions of reviews on a Chinese food delivery service platform (FDS), Dianping, Yelp, and Amazon. Our empirical analysis suggests, in general, word counts of online review contents do not obey Benford’s law, although Benford’s law holds among high-quality reviews. Deviation from Benford’s law is larger in emerging markets compared with mature online marketplaces. Further analyses reveal that positive reviews, especially positive and extreme reviews, exhibit more deviation from Benford’s law.

Open-access platform to synthesize knowledge of ape conservation across sites.

Despite the large body of literature on ape conservation, much of the data needed for evidence-based conservation decision-making is still not readily accessible and standardized, rendering cross-site comparison difficult. To support knowledge synthesis and to complement the IUCN SSC Ape Populations, Environments and Surveys database, we created the A.P.E.S. Wiki (https://apeswiki.eva.mpg.de), an open-access platform providing site-level information on ape conservation status and context. The aim of this Wiki is to provide information and data about geographical ape locations, to curate information on individuals and organizations active in ape research and conservation, and to act as a tool to support collaboration between conservation practitioners, scientists, and other stakeholders. To illustrate the process and benefits of knowledge synthesis, we used the momentum of the update of the conservation action plan for western chimpanzees (Pan troglodytes verus) and began with this critically endangered taxon. First, we gathered information on 59 sites in West Africa from scientific publications, reports, and online sources. Information was compiled in a standardized format and can thus be summarized using a web scraping approach. We then asked experts working at those sites to review and complement the information (20 sites have been reviewed to date). We demonstrate the utility of the information available through the Wiki, for example, for studying species distribution. Importantly, as an open-access platform and based on the well-known wiki layout, the A.P.E.S. Wiki can contribute to direct and interactive information sharing and promote the efforts invested by the ape research and conservation community. The Section on Great Apes and the Section on Small Apes of the IUCN SSC Primate Specialist Group will guide and support the expansion of the platform to all small and great ape taxa. Similar collaborative efforts can contribute to extending knowledge synthesis to all nonhuman primate species.

A French-Greek Cross-Site Comparison Study of the Use of Automatic Video Analyses for the Assessment of Autonomy in Dementia Patients.

Background: At present, the assessment of autonomy in daily living activities, one of the key symptoms in Alzheimer’s disease (AD), involves clinical rating scales. Methods: In total, 109 participants were included. In particular, 11 participants during a pre-test in Nice, France, and 98 participants (27 AD, 38 mild cognitive impairment—MCI—and 33 healthy controls—HC) in Thessaloniki, Greece, carried out a standardized scenario consisting of several instrumental activities of daily living (IADLs), such as making a phone call or preparing a pillbox while being recorded. Data were processed by a platform of video signal analysis in order to extract kinematic parameters, detecting activities undertaken by the participant. Results: The video analysis data can be used to assess IADL task quality and provide clinicians with objective measurements of the patients’ performance. Furthermore, it reveals that the HC statistically significantly outperformed the MCI, which had better performance compared to the AD participants. Conclusions: Accurate activity recognition data for the analyses of the performance on IADL activities were obtained.

Do farmers care about pollinators? A cross-site comparison of farmers’ perceptions, knowledge, and management practices for pollinator-dependent crops

ABSTRACT Pollinator conservation has become a key challenge to achieve sustainable agricultural landscapes and safeguard food supplies. Considering the potential negative effects of pollinator decline, international efforts have been developed to promote agri-environmental measures and pollinator-friendly management practices. However, little effort has been devoted to farmers’ perceptions and knowledge about pollinators, or to farmers’ role in enhancing pollination. We administered 376 face-to-face questionnaires in four areas of Spain with different dominant pollinator-dependent crops, to assess the factors behind farmers’ perceptions, knowledge, and practices adopted to promote pollination. Overall, 92.7% of the respondents recognized that pollinator insects are necessary for crop production, and 73.4% perceived pollinator decline in their farms. We found that farmers had moderate knowledge about pollinators (6.1 ± 1.8, on a 1–10 scale). The most applied practices to promote pollinators were reducing insecticide spraying (53.2% of respondents), diversifying crops (42.8%), and increasing fallow fields (39.1%). Factors such as education, age, concern about the pollinator crisis, and professional dedication to agriculture strongly influenced farmers’ knowledge and current application of pollinator-friendly practices. Implications of our results for the ongoing reform of the Common Agricultural Policy are discussed, highlighting the need to increase engagement and trust of farmers through communication and technical assistance.

Exploring land-use histories of tree-crop landscapes: a cross-site comparison in the Mediterranean Basin

Agroforestry landscapes in the Mediterranean Basin have emerged in a co-evolution between humans and nature and provide numerous ecosystem services to society. Tree crops are iconic elements of these landscapes and have frequently been managed in a sustainable way over centuries, shaping multifunctional landscapes and local people’s cultural identities. However, many Mediterranean tree-crop landscapes are undergoing substantial land-use changes, threatening important ecosystem services as a result. The overarching goal of this study is to explore common and diverging patterns of land-use change across different tree crops (oaks, chestnuts, olives) and contrasting landscapes in the Mediterranean Basin over a 200-year period. Specifically, we aim to: (1) describe the dominant land-use change processes across these three crop types using three exemplary sites per crop; and (2) identify and classify the main drivers that determine these landscapes’ land change histories. We find a general acceleration of landscape dynamics and identify expansion, continuity, polarisation, intensification, abandonment and renaissance as dominant processes. Although each landscape history is contextualised, we observe a general trend from multifunctional tree-crop landscapes (expansion) towards intensification or abandonment in the last 70 years. The landscapes of the southern fringe of the Mediterranean Basin show predominant trends towards intensification, while the northern landscapes evolve towards abandonment. The driving forces identified are diverse and interrelated, comprising sets of socio-cultural, political, technical, economic and natural factors. We offer some key lessons for sustainable landscape management in highlighting the undervalued potential of tree crops, the inherent complexity of landscapes, the interdependencies of drivers and the importance of economic and socio-cultural driving forces.

Do executive functions mediate the link between socioeconomic status and numeracy skills? A cross-site comparison of Hong Kong and the United Kingdom

In the fields of education, sociology, and economics, there is a long-standing connection between socioeconomic status (SES) and school outcomes in a wide variety of cultural settings, but these studies have yet to examine the possible mediating effects of domain-general cognitive factors such as executive functions (EFs). Addressing this gap and building on evidence for links between EFs and numeracy, the current cross-cultural study used a large sample (N = 835) of 9- to 16-year-old children from Hong Kong and the United Kingdom to examine the independence and interplay of SES and EFs as predictors of numeracy skills. Our analyses yielded three key findings, namely that (a) EFs consistently predicted numeracy skills across sites and genders, (b) associations between SES and EFs differed by site and gender, and (c) associations between numeracy skills and SES/EFs differed by site and gender. Together with previous findings, our results suggest culture-specific associations among SES, EFs, and numeracy, indicating that cultural insights may enable impactful shifts in public policy to narrow the achievement gap between children from affluent and disadvantaged families.

Impacts of different radiometric calibration methods on the retrievals of sun-induced chlorophyll fluorescence and its relation to productivity for continuous field measurements

Sun-induced chlorophyll fluorescence (SIF), now observed from space on a global scale, has been shown to be a powerful proxy for photosynthetic activity. Long-term in situ field canopy SIF measurements are improving, enabling better understanding of SIF signal and support to satellite missions. Since SIF retrievals rely on the absolute irradiance radiance measurements, accurate outdoor radiometric calibration is necessary to provide accurate radiance data, especially for long-term continuous measurements on forest stands. However, standard laboratory calibration methods are not practical to be performed for outdoor long-term measurements, and several in-field alternative methods are generally used, which may result in some uncertainties. Here we evaluate the effects of different radiometric calibration methods on SIF retrievals and its relation with gross primary productivity (GPP) at the canopy level. Three widely used methods are used, i.e., well-established laboratory methods with integrating sphere, laboratory-calibrated reference spectrometer (ASD FieldSpec Pro), and a light calibration source with a white reference panel. Our results indicate that different radiometric calibrations have marginal effects on vegetation indices but have significant effects on the SIF absolute value, and have slight effects on diurnal and seasonal patterns of SIF. Moreover, the relationships between GPP and SIF retrieved using different calibration coefficients are similar at both diurnal and seasonal scales, but with different regression slopes. Therefore, we recommend that a standard laboratory radiometric calibration is conducted using an integrating sphere before installation in the field and a regular in-field calibration with a well-calibrated spectrometer and white reference, especially for long-term observation above a forest canopy. Our findings have strong implications for the ongoing and future ground canopy SIF measurements and suggest the need for a consistent radiometric calibration method, especially for cross-site comparison studies.

The multiple meanings of prosperity and poverty: a cross-site comparison from Tanzania

ABSTRACT Assets are important to local definitions of poverty and wealth in rural Africa. Yet their use in asset indices can miss locally valued change. We present data from 17 villages across Tanzania to explore differences in the meaning of wealth and poverty across the country. Despite limitations in our site selection we found considerable diversity that makes a single asset index difficult to compile. Current abbreviated asset indices risk counting assets that do not matter locally.

Interpreting the oral condition in medieval European populations from a bioarchaeological perspective

Interpretation of dental ‘health’ in archaeologically derived skeletal assemblages is challenging due to the lack of patient histories, clearly understood pathological processes, broad etiologies, and cultural perceptions of health. Furthermore, the language used in description of pathological conditions of the oral cavity condition is not consistent across researchers thereby resulting in challenging cross-site comparison. Standardization of terms and description is necessary as proposed by Pilloud and Fancher (2018). This paper demonstrates the challenges associated with cross-site comparisons through an attempt to place medieval Transylvanian Székely peoples’ oral status within a larger medieval cultural and biological framework. To do this, first, a review of medieval perceptions of dental health and treatment is provided. Next, a total of 90 individuals recovered from two medieval Székely cemeteries were analyzed for age, sex, and pathological conditions of the oral cavity. The results of the analysis were then compared to other medieval skeletal assemblages reporting on dental ‘health’. Finally, a discussion of how the Székely compare to other medieval sites and the challenges faced are presented thus supporting Pilloud and Fancher’s (2018) call for standardization.

STEM education centers: catalyzing the improvement of undergraduate STEM education

BackgroundWith the remarkable attention being paid to STEM education nationally, with the growing engagement of universities and colleges in STEM education reform, and with the rise of STEM education centers, SECs, assisting universities as they strive to achieve these reforms, this research provides insight into the roles of six SECs. Through a multi-dimensional cross-site comparison, we provide a lens into the ways in which SECs function on their campuses, illuminating possibilities for those seeking to strengthen undergraduate STEM education.ResultsSECs play an important networking role on their campuses, where they inform and unify institutional efforts, serving to elevate their visibility and importance both internally and externally. Through their scholarship, SECs contribute to the knowledge base and provide funding, which add resources and incentives for the implementation of evidence-based instructional practices (EBIPs) and STEM education research. SECs augment these efforts with the assessment and evaluation of learning outcomes and curricular innovations. Additionally, SECs act as an internal resource for faculty and instructors providing programs and training to foster the application of EBIPs in STEM courses. Several SECs provide the infrastructure for broader impact activities, and act as an external funding resource.ConclusionsSTEM education centers make key contributions to their institutional environments. While the individual roles of these SECs on their campuses are distinctly unique, an in-depth look across six SECs reveals common areas of focus that allow these centers to enhance the undergraduate teaching and learning experience. Our results suggest that the ability of SECs to link STEM education research with teaching and learning initiatives provides a breadth of impact and attention across organizational levels. The analysis describes the ways in which these centers support institutional goals for undergraduate STEM education and relates these to areas of national priority. This research was carried out as part of a broader study, which informs the organizers of NSEC, the network of STEM education centers.

Steering operational synergies in terrestrial observation networks: opportunity for advancing Earth system dynamics modelling

Abstract. Advancing our understanding of Earth system dynamics (ESD) depends on the development of models and other analytical tools that apply physical, biological, and chemical data. This ambition to increase understanding and develop models of ESD based on site observations was the stimulus for creating the networks of Long-Term Ecological Research (LTER), Critical Zone Observatories (CZOs), and others. We organized a survey, the results of which identified pressing gaps in data availability from these networks, in particular for the future development and evaluation of models that represent ESD processes, and provide insights for improvement in both data collection and model integration. From this survey overview of data applications in the context of LTER and CZO research, we identified three challenges: (1) widen application of terrestrial observation network data in Earth system modelling, (2) develop integrated Earth system models that incorporate process representation and data of multiple disciplines, and (3) identify complementarity in measured variables and spatial extent, and promoting synergies in the existing observational networks. These challenges lead to perspectives and recommendations for an improved dialogue between the observation networks and the ESD modelling community, including co-location of sites in the existing networks and further formalizing these recommendations among these communities. Developing these synergies will enable cross-site and cross-network comparison and synthesis studies, which will help produce insights around organizing principles, classifications, and general rules of coupling processes with environmental conditions.

Dimensions of Landscape Stewardship across Europe: Landscape Values, Place Attachment, Awareness, and Personal Responsibility

Improved perceptions towards landscape stewardship, at the local level, could help achieve more sustainable futures. However, little research has been done on the dimensions of landscape stewardship underlying such perceptions. Here we look at the perception of landscape values, place attachment, awareness of the adverse consequences human action might have on landscapes, and ascription of personal responsibility across Europe as well as how these dimensions are connected and influenced by personal capabilities and socio-cultural contexts. We conducted a cross-site comparison study, in six European municipalities, using a survey to capture residents’ levels of awareness, responsibility, and attachment as derived from a set of statements. Respondents were also asked to indicate the values they perceive in the local landscape from a given list. The data was analysed by combining frequency analysis, factor analysis, and contingency tables. In our sample of 726 respondents, stronger awareness was related to stronger ascription of personal responsibility, but a connection to place attachment was not clear. Perception of multiple landscape values was related to stronger awareness, responsibility, and place attachment. Meanwhile, awareness and responsibility were influenced by respondents’ occupation, levels of income and education, and socio-cultural context, whereas place attachment was linked to their relationship to the local area. We conclude that enhancing commitment towards landscape stewardship, at the local level, requires efforts focused on making environmental education more universal, implementing green options accessible to everyone, and people experientially engaging more actively with their local landscapes.

Reducing losses but failing to sequester carbon in soils – the case of Conservation Agriculture and Integrated Soil Fertility Management in the humid tropical agro-ecosystem of Western Kenya

Agriculture is a global contributor to greenhouse gas emissions, causing climate change. Soil organic carbon (SOC) sequestration is seen as a pathway to climate change mitigation. But, long-term data on the actual contribution of tropical soils to SOC sequestration are largely absent. To contribute to filling this knowledge gap, we measured SOC in the top 15cm over 12 years in two agronomic long-term trials in Western Kenya. These trials include various levels – from absence to full adoption – of two widely promoted sustainable agricultural management practices: Integrated Soil Fertility Management (ISFM; i.e. improved varieties, mineral fertilizer and organic matter/manure incorporation) and Conservation Agriculture (CA; improved varieties, mineral fertilizer, zero-tillage and crop residues retention). None of the tested ISFM and CA treatments turned out successful in sequestering SOC long-term. Instead, SOC decreased significantly over time in the vast majority of treatments. Expressed as annual averages, losses ranged between 0.11 and 0.37tCha−1 yr−1 in the CA long-term trial and 0.21 and 0.96tCha−1 yr−1 in the ISFM long-term trial. Long-term application of mineral N and P fertilizer did not mitigate SOC losses in both trials. Adopting zero-tillage and residue retention alone (as part of CA) could avoid SOC losses of on average 0.13tCha−1 yr−1, while this was 0.26tCha−1 yr−1 in response to mere inclusion of manure as part of ISFM. However, cross-site comparison disclosed that initial SOC levels of the two trials were different, probably as a result of varying land use history. Such initial soil status was responsible for the bulk of the SOC losses and less so the various tested agronomic management practices. This means, while ISFM and CA in the humid tropical agro-ecosystem of Western Kenya contribute to climate change mitigation by reducing SOC losses, they do not help offsetting anthropogenic greenhouse gas emissions elsewhere.

Participatory mapping of landscape values in a Pan-European perspective

Human–nature interactions are reflected in the values people assign to landscapes. These values shape our understanding and actions as landscape co-creators, and need to be taken into account to achieve an integrated management of the landscape that involves civil society. The aim of this research was to increase the current knowledge on the most and least common landscape values perceived by local stakeholders, the patterns in the spatial distribution of values, and their connection to different socio-economic backgrounds and landscape characteristics across Europe. The research consisted of a cross-site comparison study on how landscape values are perceived in six areas of Europe using Public Participation GIS surveys. Answers were analysed combining contingency tables, spatial autocorrelation and bivariate correlation methods, kernel densities, land cover ratios, and viewshed analyses. Results were discussed in the light of findings derived from other European participatory mapping studies. We identified shared patterns in the perception of landscape values across Europe. Recreation, aesthetics, and social fulfilment were the most common values. Landscape values showed common spatial patterns mainly related to accessibility and the presence of water, settlements, and cultural heritage. However, respondents in each study site had their own preferences connected to the intrinsic characteristics of the local landscape and culture. The results encourage land planners and researchers to approach landscape values in relation to socio-cultural and bio-physical land characteristics comprehensibly, acknowledging the complexity in the relationship between people’s perception and the landscape, to foster more effective and inclusive landscape management strategies.

Merging science into community adaptation planning processes: a cross-site comparison of four distinct areas of the Lower Mekong Basin

Climate change is increasingly affecting rural areas worldwide. The Lower Mekong Basin (LMB) is at particular risk due to heat stress, changing rainfall patterns, rising sea levels, and more frequent and extreme climatic events. It is imperative that local-level adaptation plans are developed in a manner that builds resilience to these growing threats. Strategies for developing adaptation plans tend to comprise predominantly science-led or predominantly community-led processes. This study examines an approach that balances inputs from both processes in characterizing community vulnerability as a component of the adaptation planning workflow. Evaluation sites are located within four distinct sub-regions of the LMB: the Vietnam Mekong Delta, the Annamite Mountains of Lao PDR, the Cambodia central lowlands, and the mid-elevation forests of northern Thailand. Our results indicate that by merging science-based data with community-level perspective, knowledge gaps from both sides are filled and a more comprehensive understanding of vulnerability is factored into adaptation planning.

Modeling gross primary production of paddy rice cropland through analyses of data from CO2 eddy flux tower sites and MODIS images

Accurate information on the gross primary production (GPP) of paddy rice cropland is critical for assessing and monitoring rice growing conditions. The eddy co-variance technique was used to measure net ecosystem exchange (NEE) of CO2 between paddy rice croplands and the atmosphere, and the resultant NEE data then partitioned into GPP (GPPEC) and ecosystem respiration. In this study, we first used the GPPEC data from four paddy rice flux tower sites in South Korea, Japan and the USA to evaluate the biophysical performance of three vegetation indices: Normalized Difference Vegetation Index (NDVI); Enhanced Vegetation Index (EVI), and Land Surface Water Index (LSWI) in terms of phenology (crop growing seasons) and GPPEC, which are derived from images taken by Moderate Resolution Imaging Spectroradiometer (MODIS) sensors. We also ran the Vegetation Photosynthesis Model (VPM), which is driven by EVI, LSWI, photosynthetically active radiation (PAR) and air temperature, to estimate GPP over multiple years at these four sites (GPPVPM). The 14 site-years of simulations show that the seasonal dynamics of GPPVPM successfully tracked the seasonal dynamics of GPPEC (R2>0.88 or higher). The cross-site comparison also shows that GPPVPM agreed reasonably well with the variations of GPPEC across both years and sites. The simulation results clearly demonstrate the potential of the VPM model and MODIS images for estimating GPP of paddy rice croplands in the monsoon climates of South Korea and Japan and the Mediterranean climate in California, USA. The application of VPM to regional simulations in the near future may provide crucial GPP data to support the studies of food security and cropland carbon cycle around the world.

Two-Site Evaluation of the Repeatability and Precision of an Automated Dual-Column Hydrogen/Deuterium Exchange Mass Spectrometry Platform.

Hydrogen/deuterium exchange coupled with mass spectrometry (HDX-MS) is an information-rich biophysical method for the characterization of protein dynamics. Successful applications of differential HDX-MS include the characterization of protein-ligand binding. A single differential HDX-MS data set (protein ± ligand) is often comprised of more than 40 individual HDX-MS experiments. To eliminate laborious manual processing of samples, and to minimize random and gross errors, automated systems for HDX-MS analysis have become routine in many laboratories. However, an automated system, while less prone to random errors introduced by human operators, may have systematic errors that go unnoticed without proper detection. Although the application of automated (and manual) HDX-MS has become common, there are only a handful of studies reporting the systematic evaluation of the performance of HDX-MS experiments, and no reports have been published describing a cross-site comparison of HDX-MS experiments. Here, we describe an automated HDX-MS platform that operates with a parallel, two-trap, two-column configuration that has been installed in two remote laboratories. To understand the performance of the system both within and between laboratories, we have designed and completed a test-retest repeatability study for differential HDX-MS experiments implemented at each of two laboratories, one in Florida and the other in Spain. This study provided sufficient data to do both within and between laboratory variability assessments. Initial results revealed a systematic run-order effect within one of the two systems. Therefore, the study was repeated, and this time the conclusion was that the experimental conditions were successfully replicated with minimal systematic error.