Cross-infection In Dentistry Research Articles

Enhancing Dental Alginate with Syzygium aromaticum, Zingiber officinale and Green Silver Nanoparticles: A Nature-Enhanced Approach for Superior Infection Control.

The study addresses the challenge of cross-infection in dentistry, focusing on improving disinfection protocols for dental hydrocolloid gel materials. This research aimed to incorporate chlorhexidine, natural plant extracts, and green-synthesized silver nanoparticles (AgNPs) into dental alginate to enhance safety and hygiene standards in dental practices. Conventional dental alginate served as the control, with experimental groups including alginate modified with 0.2% chlorhexidine (CHX-group) and alginate incorporating AgNPs synthesized from Syzygium aromaticum (SA) and Zingiber officinale (ZO) extracts (SA + AgNPs and ZO + AgNPs groups). Plant extracts were analyzed via GC/MS to confirm their composition. UV-visible spectroscopy, SEM, and EDX characterized the synthesized AgNPs. Antimicrobial efficacy against Candida albicans, Streptococcus mutans, and methicillin-resistant and methicillin-sensitive Staphylococcus aureus was evaluated using agar well diffusion assays. The dimensional accuracy of alginate impressions was assessed according to ISO 21563:2021 standards. Chemical analysis of SA and ZO extracts identified 60 and 43 active compounds, respectively, supporting their use in AgNP synthesis. UV-visible spectroscopy, SEM, and EDX confirmed the formation of spherical AgNPs using SA and ZO extracts. Modified groups showed inhibitory activity against Streptococcus mutans and methicillin-resistant (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA), in contrast to the unmodified control. Both AgNP-modified groups demonstrated efficacy comparable to the CHX-group against MRSA and MSSA, with SA + AgNP showing superior performance against C. albicans. The dimensional accuracy of all groups was within clinically acceptable ranges as reported in the literature (0.027-0.083 mm). CHX, silver nitrate, and green-synthesized AgNPs present promising options for developing self-disinfecting alginate impression materials. Utilizing plant extracts in AgNP synthesis offers a safe, efficient, and synergistic approach between metal ions and phytotherapeutic agents. This approach could potentially enhance disinfection efficacy without compromising material performance, improving dental safety and hygiene.

Pyrosequencing analysis of bacterial community changes in dental unit waterlines after chlorogenic acid treatment.

The contamination of dental unit waterlines (DUWLs) poses a significant risk of cross-infection in dentistry. Although chemical disinfectants have been effective in reducing number of bacteria, they do have limitations. This study aimed to investigate the potential of chlorogenic acid, a natural substance with broadspectrum antibacterial properties, for treating DUWLs. Over a period of three months, we analyzed the microbial communities in 149 DUWLs samples collected from 5 dental units using high-throughput pyrophosphate sequencing. The results revealed that chlorogenic acid treatment had a significant impact on the microbial community profile in the DUWLs, with the most significant changes occurring within the first 15 days and stabilization observed in the last 30 days. The predominant genera detected in the samples were Bacteroides, Lactobacillus, Streptococcus, Methylobacterium, and Phreatobacter. Additionally, the relative abundance of certain beneficial bacteria, such as Alloprevotella, Roseburia, and Blautia, increased, while the presence of opportunistic pathogens like Mycobacteria significantly decreased. The functional prediction analysis using the KEGG database indicated a decrease in the pathogenicity of the bacterial community in the DUWLs following chlorogenic acid treatment. This study introduces a novel approach for the prevention and treatment of infections associated with dental care.

EFFECT OF IMMERSION TYPE III GYPSUM IN SODIUM HYPOCHLORITE AND POVIDONE-IODINE SOLUTIONS ON THE NUMBER OF CANDIDA ALBICANS COLONIES

Introduction: Cross-infection is the transfer of microorganisms that can occur from individual to individual or to object. Candida albicans is one of the infectious pathogens that can cause cross-infection in dentistry. One example is the manufacture of working models of gypsum material, which can cause cross-infection from patients to dentists or technicians, so it is necessary to control infection by means of disinfection of type III gypsum material work models. Objective: To determine the effectiveness of soaking type III Gypsum in a 0.5% Sodium hypochlorite and 2% Povidone Iodine solution against the growth of Candida albicans. Methods: A laboratory experiment with a total of 21 samples, consisting of 7 samples for each group. The sample is made of a type III gypsum plate measuring 2 x 1 x 0.5 cm. The sample consisted of 3 groups: treatment 1, treatment 2, and treatment 3. The study used the diffusion method. Gypsum plate type III was contaminated with Candida albicans and then immersed in a solution of sterile distilled water (C), 0.5% sodium hypochlorite (P1), and 2% povidone-iodine (P2). The soaking results were planted in SDA media, incubated for 2x24 hours, and then counted using a colony counter. The data were analyzed using Kruskal- Wallis and Mann-Whitney post hoc analyses. Results: In the Kruskal- Wallis analysis of significance (p), between each group, there was a significant difference (p<0.05). The Mann-Whitney post hoc analysis test showed a correlation between group K and P1 and P2 (p<0.05). Conclusion: 0.5% Sodium Hypochlorite and 2% Povidone Iodine solutions are effective as disinfectants using the immersion method in Type III Gypsum against the growth of Candida albicans.

Cross Infection in Dentistry and the Dental Aerosols –A Potential Health Hazard

Many hazards are often associated with different occupations and known as occupational hazards. Dentistry is also one of those professions having many occupational risks. Such hazards are broadly categorized into chemical, biological, physical, mechanical and psychological factors. Cross infection is one of such hazards faced by the health care providers’ particularly dental workers. Cross infection is the transmission of infectious agents between patient and staff in clinical environment. Control of cross infection has remained the major concern of the dental community for decades. Various vectors are involved in cross infections in Dentistry including patients, environment (water, air), instruments etc. Particularly among the environmental factors, airborne route is one of the major routes of cross contamination and so aerosols produced in dental environment carry greatest pathogenic potential. Production of aerosols and splatters occur because of many procedures carried out in mouth including crown preparation, ultrasonic scaling, caries excavation etc. Patients, dental instruments and dental unit waterlines (DUWL) are the important sources of contaminated aerosol production in dental operatory. Use of personal Protective equipments, regular use of pre-procedural mouth rinses and high evacuation devices are few of the suggested methods to reduce risk of cross contamination especially through aerosols and splatters in the dental offices. The related articles were searched through PubMed and Google Scholar.

STERILIZATION & CROSS INFECTION IN DENTISTRY

Objectives: This study aims to assess the knowledge, perception and attitudeof patients towards sterilization and cross Infection and to assess the effects of education onperception and behavior towards sterilization and cross infection. Cross infection control ismost important in dentistry as it is considered one of the main reasons of rapid spread ofinfectious diseases now a days. Setting: Major public sector hospital of Punjab Pakistan, PunjabDental Hospital Lahore. Material & Method: We surveyed patients in OPDs of Punjab DentalHospital Lahore. Survey questions covered demographics as well as 15 questions related tosterilization and cross infection in dentistry. Questionnaires were given to educated peopleand interviews were taken from uneducated or less educated people. Data was collected andanalyzed. Results: patients have some knowledge about sterilization and cross infection buttheir concepts need a lot of improvement. Patients know that microbial contamination occursbut they are unaware of the diseases that are transmitted. Similarly on other perspective thereis discrepancy in knowledge and practice, as they do not take it seriously or they are unawareof the consequences of cross infection or long term effects of HIV, Hepatitis B & C. Disposableinstruments are very important for controlling cross infection. Only 60% people said they wouldprefer disposable instruments over reusable instruments when option is given to them. Mostpeople are reluctant to be treated with disposable instruments due to extra cost on treatment.92% were well aware of significance of hand washing of dentist before and after dental checkup,but when they go through dental examination they are either careless or hesitant to ask dentistto wash hands. Conclusion: Besides the knowledge there are other factors like patientsattitude and concerns also play vital role in preventing infection transmission in them. We haveto educate them about infectious diseases and their root of transmission and their long termeffects on health.

PATIENTS PERCEPTION

Objectives: This study aims to assess the knowledge, perception and attitudeof patients towards sterilization and cross Infection and to assess the effects of education onperception and behavior towards sterilization and cross infection. Cross infection control ismost important in dentistry as it is considered one of the main reasons of rapid spread ofinfectious diseases now a days. Setting: Major public sector hospital of Punjab Pakistan, PunjabDental Hospital Lahore. Material & Method: We surveyed patients in OPDs of Punjab DentalHospital Lahore. Survey questions covered demographics as well as 15 questions related tosterilization and cross infection in dentistry. Questionnaires were given to educated peopleand interviews were taken from uneducated or less educated people. Data was collected andanalyzed. Results: patients have some knowledge about sterilization and cross infection buttheir concepts need a lot of improvement. Patients know that microbial contamination occursbut they are unaware of the diseases that are transmitted. Similarly on other perspective thereis discrepancy in knowledge and practice, as they do not take it seriously or they are unawareof the consequences of cross infection or long term effects of HIV, Hepatitis B & C. Disposableinstruments are very important for controlling cross infection. Only 60% people said they wouldprefer disposable instruments over reusable instruments when option is given to them. Mostpeople are reluctant to be treated with disposable instruments due to extra cost on treatment.92% were well aware of significance of hand washing of dentist before and after dental checkup,but when they go through dental examination they are either careless or hesitant to ask dentistto wash hands. Conclusion: Besides the knowledge there are other factors like patientsattitude and concerns also play vital role in preventing infection transmission in them. We haveto educate them about infectious diseases and their root of transmission and their long termeffects on health.

Healthcare-associated viral and bacterial infections in dentistry

Infection prevention in dentistry is an important topic that has gained more interest in recent years and guidelines for the prevention of cross-transmission are common practice in many countries. However, little is known about the real risks of cross-transmission, specifically in the dental healthcare setting. This paper evaluated the literature to determine the risk of cross-transmission and infection of viruses and bacteria that are of particular relevance in the dental practice environment. Facts from the literature on HSV, VZV, HIV, Hepatitis B, C and D viruses, Mycobacterium spp., Pseudomonas spp., Legionella spp. and multi-resistant bacteria are presented. There is evidence that Hepatitis B virus is a real threat for cross-infection in dentistry. Data for the transmission of, and infection with, other viruses or bacteria in dental practice are scarce. However, a number of cases are probably not acknowledged by patients, healthcare workers and authorities. Furthermore, cross-transmission in dentistry is under-reported in the literature. For the above reasons, the real risks of cross-transmission are likely to be higher. There is therefore a need for prospective longitudinal research in this area, to determine the real risks of cross-infection in dentistry. This will assist the adoption of effective hygiene procedures in dental practice.

Public perception of cross-infection control in dentistry.

Since the advent of HIV/AIDS at the beginning of the 1980s, concern has generated considerable impetus for change in cross-infection control procedures in dentistry. This process has been hastened partly by media coverage which, in tending to favour sensation over rational discourse, has played a not inconsiderable role in shaping public understanding and expectations. This study aimed to investigate public perceptions of cross-infection control in dentistry in Australia using a postal follow-up to the 1995 National Dental Telephone Interview Survey. The postal survey response rate was 85.2 per cent. Concerns about the procedures used by their dentist to sterilize instruments were reported by 13.3 per cent of respondents overall, and this was greater among non-health-card-holders, individuals who mainly spoke a language other than English in the home, and those who reported a non-routine dental visiting pattern. Avoidance or delaying of dental visits due to the perceived cross-infection risk was reported by an overall 3.6 per cent of people, and this was higher among females and those who expressed concern about cross-infection control. The profession has a responsibility to ensure that information on the measures which have been taken to reduce the risk of cross-infection in dentistry is disseminated as widely and as clearly as possible so that undue public concern and avoidance of dental care are minimized.

Role of ultrasonic cleaning in control of cross-infection in dentistry

The dye/paper method of mapping ultrasound fields has been used to demonstrate the non-uniform distribution of energy density and cavitational activity in an ultrasonic cleaning bath. It is also shown that removal of a layer of artificial contaminant from a surface proceeds at rates which vary considerably with position because of standing waves set up by reflections in the walls of the bath and at the liquid surface. In view of the use of such baths as part of the process of controlling cross-infection in dentistry, it is suggested that manual cleaning of reusable instruments may be necessary as well.

The prevention of cross-infection. Progress in the West Midlands.

A questionnaire was sent to a group of general dental practitioners in the West Midlands, one year after they had participated in a previous survey and a year following publication of the British Dental Association's 'Guide to blood-borne viruses and the control of cross-infection in dentistry'. Results show that awareness of the need to safeguard patients and staff from the risks of cross-infection continues to grow and that procedures undertaken to reduce the possibility of cross-infection continue to be improved.

The control of cross-infection in dentistry.

The control of cross-infection in dentistry.

Cross Infection in Dentistry - Report of O.M.I.G. Workshop 1987: How Far is it Realistic to go With Precautions Against Cross-Infection in Dentistry?

(1988). Cross Infection in Dentistry—Report of O.M.I.G. Workshop 1987: How Far is it Realistic to go With Precautions Against Cross-Infection in Dentistry? Microbial Ecology in Health and Disease: Vol. 1, No. 2, pp. 139-145.

Cross Infection in Dentistry—Report of O.M.I.G. Workshop 1987: How Far is it Realistic to go With Precautions Against Cross-Infection in Dentistry?

Cross Infection in Dentistry—Report of O.M.I.G. Workshop 1987: How Far is it Realistic to go With Precautions Against Cross-Infection in Dentistry?

The problems of cross-infection in dentistry.

The problems of cross-infection in dentistry.

The prevention of cross-infection in dentistry

The prevention of cross-infection in dentistry

The prevention of cross-infection in dentistry.

The prevention of cross-infection in dentistry.

Cross-infection in dentistry with particular reference to oral surgery and periodontics

There is evidence that the dentist, his assistant and patients are at risk from aerosol and particulate matter created during prophylaxis and oral and periodontal surgical procedures. Methods of reducing these hazards are discussed.

Cross-infection in dentistry

Punica granatum has been used for many years in folk medicine due to several purposes. The aim of the present study was to evaluate the effect of methanolic extract of Punica granatum peel (MEPGP) against Streptococcus mutans, Staphylococcus aureus, Streptococcus salivarius, Streptococcus sanguinis, Staphylococcus epidermidis, Actynomyces viscosus, Lactobacillus acidophilus and Candida albicans.In this in vitro study, the mentioned oral organisms were cultured in blood agar and mueller-hinton media and then paper disks containing MEPGP at concentrations of 4 mg/ml, 8 mg/ml and 12 mg/ml were inserted on medias. The antimicrobial activity was evaluated by agar disk diffusion method. The effects of three different concentrations of MEPGP against microorganisms were compared using one-way ANOVA and Tukey tests.All concentrations of MEPGP had antibacterial activity against S. aureus and S. epidermidis. Only at concentration of 8 mg/ml and 12 mg/ml MEPGP was effective against L. acidophilus, S. mutans and S. salivarius. Furthermore; no concentrations of MEPGP inhibited A. viscosus and C. albicans.This study suggests that MEPGP might be used as an antibacterial agent in controlling oral infections.