Crop Production Research Articles

Confirmation of synthetic auxin herbicide resistance in a green pigweed (Amaranthus powellii) population from Ontario, Canada

Abstract Following the application of MCPA/MCPB at 1.7 kg ae ha−1 at a field site near Dresden, ON, Canada, poor control (<50% visible control) of green pigweed (Amaranthus powellii S. Watson) was observed. Amaranthus powellii is a common weed in Ontario crop production, and its evolution of resistance to synthetic auxin herbicides (SAHs) could pose a risk to crop yields. The suspected resistant A. powellii population (R) was used in dose–response and field experiments to determine resistance to SAHs. The objective of these studies was to determine whether this population of A. powellii is resistant to MCPA and cross-resistant to other SAHs. The GR50 (herbicide dose that causes a 50% reduction in plant aboveground biomass) values were determined by fitting plant dry weight data, obtained following application with seven SAHs, to a four-parameter log-logistic equation and were compared between the suspected-resistant (R) population and a known susceptible (S) population of A. powellii. The field trial was conducted in 2017, 2018, 2019, and 2021 in corn (Zea mays L.) and consisted of 11 postemergence SAH treatments. The GR50 values differed between the R and S populations following application with MCPA, aminocyclopyrachlor, dichlorprop-p, and mecoprop, resulting in resistance factors of 4.4, 3.0, 2.5, and 2.4, respectively. In the field study, dicamba and MCPA ester controlled A. powellii 84% and 30%, respectively, at 8 wk after treatment application (WAA). The control of Amaranthus powellii with all SAHs applied POST in corn was poor (<90% visible control) at 8 WAA. Both studies confirmed resistance to SAHs in this population of A. powellii, which will create limitations for farmers aiming to control this weed.

Reinforcing Nitrogen Nutrition Through Partial Substitution with Organic Nitrogen Enhances the Properties of Natural Rubber

The partial substitution of chemical fertilizer with organic fertilizer is a crucial practice for enhancing crop production and quality, although its impact on natural rubber has rarely been explored. In this study, a two-year field experiment was conducted to investigate the impact of different nitrogen application rates and varying proportions of organic nitrogen substitution on dry rubber yield, nitrogen nutrition, and natural rubber properties. Regarding nitrogen application, the control treatment received no nitrogen amendment, while the low-nitrogen treatment was amended with 138 g·tree−1·year−1 of nitrogen. The medium-nitrogen treatment received 276 g·tree−1·year−1 of nitrogen, and the high-nitrogen treatment received 552 g·tree−1·year−1 of nitrogen. In addition, the low-organic-nitrogen substitution treatment and medium-organic-nitrogen substitution treatment were amended with 276 g·tree−1·year−1 of nitrogen each. The results demonstrated that the 50% organic nitrogen substitution treatment resulted in the highest dry rubber yield across all sampling periods, ranging from 46.43 to 94.65 g·tree−1. Additionally, this treatment exhibited superior soil total nitrogen (1067.69 mg·kg−1), available nitrogen (84.06 mg·kg−1), and nitrogen content in roots (1.08%), leaves (3.25%), fresh rubber latex (0.27%), and raw natural rubber (0.44%) compared with other treatments. In terms of the physical properties of natural rubber, the 50% organic nitrogen substitution treatment resulted in advantages in the weight-average molecular weight (1.57 × 106 g·mol−1), number-average molecular weight (0.36 × 106 g·mol−1), plasticity retention index (97.35%), Wallace plasticity (40.25), and Mooney viscosity (81.40). For mechanical properties, natural rubber from the substitution treatment exhibited higher tensile strength (19.84 MPa), greater elongation at break (834.75%), and increased tear strength (31.07 N·mm−1). Overall, the substitution of 50% chemical nitrogen fertilizer with organic nitrogen fertilizer improved nitrogen nutrition in rubber trees by introducing organic nitrogen input, resulting in remarkable enhancements in natural rubber properties. Therefore, the incorporation of organic fertilizer as a substitution for 50% of chemical fertilizer is demonstrated as an effective strategy for improving both the yield and properties of natural rubber.

Confirmation of glyphosate-resistant Palmer amaranth (Amaranthus palmeri) populations in New York and responses to alternative chemistries

Abstract Palmer amaranth (Amaranthus palmeri S. Watson, AMAPA) is one of the most troublesome weeds in North America due to its rapid growth rate, substantial seed production, competitiveness and the evolution of herbicide-resistant populations. Though frequently encountered in the South, Midwest, and Mid-Atlantic regions of the United States, A. palmeri was recently identified in soybean [Glycine max (L.) Merr.] fields in Genesee, Orange, and Steuben counties, NY, where glyphosate was the primary herbicide for in-crop weed control. This research, conducted in 2023, aimed to (1) describe the dose response of three putative resistant NY A. palmeri populations to glyphosate, (2) determine their mechanisms of resistance, and (3) assess their sensitivity to other postemergence herbicides commonly used in NY crop production systems. Based on the effective dose necessary to reduce aboveground biomass by 50% (ED50), the NY populations were 42 to 67 times more resistant to glyphosate compared with a glyphosate-susceptible population. Additionally, the NY populations had elevated EPSPS gene copy numbers ranging from 25 to 135 located within extrachromosomal circular DNA (eccDNA). Label rate applications of Weed Science Society of America (WSSA) Group 2 herbicides killed up to 42% of the NY populations of A. palmeri. Some variability was observed among populations in response to WSSA Group 5 and 27 herbicides. All populations were effectively controlled by labeled rates of herbicides belonging to WSSA Groups 4, 10, 14, and 22. Additional research is warranted to confirm whether NY populations have evolved multiple resistance to herbicides within other WSSA groups and to develop effective A. palmeri management strategies suitable for NY crop production.

Agricultural Sector of Ulyanovsk Region (1966-1970): A Focus on Crop Production

This article provides a comprehensive analysis of the modernization processes in the crop production sector of the Ulyanovsk region during the Eighth Five-Year Plan. The research draws on unpublished documents sourced from the State Archive of the Ulyanovsk Region and the State Archive of Contemporary History of the Ulyanovsk Region, a significant portion of which is introduced into scholarly discourse for the first time, alongside statistical materials and periodicals. The study examines the efforts of party organs and farm leadership aimed at intensifying the development of crop production. It addresses the authorities’ personnel policies regarding the expansion of vocational and technical education institutions, the organization of training courses for specialists in mass professions, and the enhancement of their qualifications to meet agricultural needs. The dynamics of crop production development from 1966 to 1970 are analyzed, evaluating the achievements of regional farms in producing key agricultural products. The author concludes that, despite facing problematic farms and a slight reduction in areas allocated for grain crops, the authorities in the Ulyanovsk region managed not only to meet but also to exceed the planned targets for yield, total harvest, and state procurement volumes of primary crop products during this period.

Biochar affects soil properties over 1 m depth in an alkaline soil of north China Plain

Biochar has been shown to enhance soil quality and agricultural yields. Previous studies about biochar's effect on soil properties mainly concentrated on the top 30 cm layer but less on the subsoils. Given the subsoil's active role in climate change mitigation and its significance for nutrient cycling and crop productivity, understanding biochar's effects at depth is crucial. This study explored the responses of soil organic carbon (SOC), total nitrogen, available potassium, available phosphorus, pH, and electrical conductivity to different doses of biochar addition (0, 10, 20, and 30 Mg/ha) over the 1 m depth of alkaline soil. Additionally, the impact of biochar on soil microbial community was assessed in the top 20 cm. Results demonstrated that biochar addition can increase SOC and improve soil properties in deep soil horizons. Specifically, a 30 Mg/ha biochar addition increased SOC by 1.2–10.1 Mg C/ha in the 10–40 cm layer and by 3 Mg C/ha in the 60–80 cm depth over two years. Additionally, biochar addition at this rate increased total nitrogen by 0.2–0.3 g N/kg in the 10–40 cm depth and elevated available potassium across the 1 m profile, with a maximum increment of 313 mg/kg in the surface 10 cm and a minimum of 97 mg/kg in the 40–60 cm depth. While biochar application did not increase available phosphorus, it resulted in a minor decrease in soil pH (<0.7 units) and a slight increase in electrical conductivity. Moreover, biochar addition did not significantly alter the soil microbial community. Our findings underscore the importance of considering subsoils when evaluating biochar's impact on soil properties. We suggest that subsoils should be considered when estimating the potential of cropland management for increasing soil carbon sequestration and improving soil conditions.

A FINANCIAL EVALUATION OF WHEAT PRODUCTION PROJECTS UNDER CENTER PIVOT IRRIGATION SYSTEMS IN ANBAR GOVERNORATE

The research aims to identify the financial feasibility of cultivating the wheat crop under modern irrigation systems in desert areas and to clarify the impact of the capacity of the systems in those projects in achieving the financial feasibility of their establishment, using the economic evaluation criteria for the projects. The research included a study of 60 projects in Anbar Governorate for the 2020-2021 production season. It has been shown that projects that use several systems, despite their high investment costs, can recover the money invested in them within a relatively short period. The projects that use modern irrigation systems covering 60 donums were among the best projects that achieved technical efficiency in the productivity of the wheat crop, and the average net income reached the cash in the sample is about 1.3 billion dinars, while the average net income per donum in the research sample is about 189 thousand dinars. The sample was not able to achieve accounting profits, The research recommended the need to use electric power systems as an alternative to relying on electric power generators, as the average price of solar power systems in the Iraqi market is currently within reach.

Examining the impact of water scarcity on agricultural output in the Gulf Cooperation Council (GCC) region

AbstractThis research paper examines the impact of water scarcity on agricultural output in the Gulf Cooperation Council (GCC) region. It highlights the increasing global water scarcity and its consequences, including the prevalence of water‐stressed areas and the adverse effects on human health and economic sectors. The paper focuses on the GCC region, known for its water scarcity and desertification, exacerbated by climate change and pollution. The study aims to investigate the relationship between water stress levels and agricultural productivity, evaluating the effects of freshwater withdrawals, fertilizer consumption, and agricultural methane emissions on crop production. Additionally, it explores the connection between agricultural output and net national income per capita. By addressing these research questions, the study contributes to the understanding of agricultural water scarcity and its implications for global food security and sustainability. The paper utilizes data from the World Bank to analyze crop production in the GCC countries from 2001 to 2020, shedding light on the influence of water scarcity on agricultural landscapes. The research highlights the efforts made by GCC countries to enhance food security, including strategic investments in agricultural lands and bilateral agreements. It also discusses the challenges faced by specific countries within the region, such as Saudi Arabia, the United Arab Emirates (UAE), and Kuwait, in terms of agricultural production and water constraints. Overall, the paper provides insights into the complexities of agricultural water scarcity and its effects on the GCC region, emphasizing the need for sustainable solutions to ensure food security and economic stability.

Assessment of sowing environment for wheat cultivars and evaluation of growing degree days for phenological stages in the Northwest Himalayan region of Himachal Pradesh

BackgroundThe date of sowing is a non-monetary input factor that plays a crucial role in field crop management. Based on the sowing time, management practices of crop production affect crop growth, development, and yield. Under varied environmental conditions, this factor may be useful in addressing low yields and combating/escaping stresses either due to diseases or due to any unfavourable environment.ResultsA field experiment was conducted during the rabi season of 2017–18 and 2018–19 to study the accumulated growing degree days (GDD) and performance of six wheat cultivars (HS 562, HD 2967, HD 3086, HI 1544, MACS 6222 and WR 544) under four sowing dates (5th November, 25th November, 15th December and 5th January). The study revealed that 5th November is the best date for sowing resulting in higher pooled grain yield of 4.85 Mg ha−1 and 3.94 Mg ha−1 at Bajaura and Malan, respectively. Significant reductions in the grain yield and yield attributes were observed when the date of sowing was set beyond 5th November. Among the cultivars tested, HS 562 gave a significantly higher pooled grain yield of 4.46 and 3.75 Mg ha−1 at Bajaura and Malan, respectively. Pooled data on physiological maturity revealed that variety HS 562 took the highest calendar days (168 days) and GDD (1573.42 °C days) to attain physiological maturity at Bajaura while at Malan HI 1544 and HD 3086 took the highest calendar days (156 and 155 days, respectively) and GDD (2055.41 and 2051.81 °C days, respectively) to attain their physiological maturity.ConclusionFrom the study, it can be concluded that the early date of sowing provides the crop with ample duration to accumulate growing degree days resulting in a higher accumulation of photosynthates resulting in significantly higher yield attributes and grain yield. Therefore, 5th November is recommended as the best time for sowing of wheat crop whereas the sowing must not be delayed beyond 25th November. HS 562 was superior among the cultivars and is recommended for Malan and Bajaura regions for attaining higher yield.

Functions of exogenous strigolactone application and strigolactone biosynthesis genes GhMAX3/GhMAX4b in response to drought tolerance in cotton (Gossypium hirsutum L.)

BackgroundDrought stress markedly constrains plant growth and diminishes crop productivity. Strigolactones (SLs) exert a beneficial influence on plant resilience to drought conditions. Nevertheless, the specific function of SLs in modulating cotton’s response to drought stress remains to be elucidated.ResultsIn this study, we assess the impact of exogenous SL (rac-GR24) administration at various concentrations (0, 1, 5, 10, 20 µM) on cotton growth during drought stress. The findings reveal that cotton seedlings treated with 5 µM exogenous SL exhibit optimal mitigation of growth suppression induced by drought stress. Treatment with 5 µM exogenous SL under drought stress conditions enhances drought tolerance in cotton seedlings by augmenting photosynthetic efficiency, facilitating stomatal closure, diminishing reactive oxygen species (ROS) generation, alleviating membrane lipid peroxidation, enhancing the activity of antioxidant enzymes, elevating the levels of osmoregulatory compounds, and upregulating the expression of drought-responsive genes. The suppression of cotton SL biosynthesis genes, MORE AXILLARY GROWTH 3 (GhMAX3) and GhMAX4b, impairs the drought tolerance of cotton. Conversely, overexpression of GhMAX3 and GhMAX4b in respective Arabidopsis mutants ameliorates the drought-sensitive phenotype in these mutants.ConclusionThese observations underscore that SLs significantly bolster cotton’s resistance to drought stress.

Isolation and Identification of Multi-Traits PGPR for Sustainable Crop Productivity Under Salinity Stress

High salinity poses a significant threat to arable land globally and contributes to desertification. Growth-promoting rhizobacteria assist plants in mitigating abiotic stresses and enhancing crop productivity through the production of siderophores, exopolysaccharides (EPS), solubilisation of phosphate, indole-3-acetic acid (IAA), and other secondary metabolites. This study aimed to isolate, identify, and characterise bacteria that exhibit robust growth-promoting properties. A total of 64 bacterial isolates from the rhizosphere of Miscanthus sinensis were evaluated for plant growth-promoting (PGP) traits, including IAA, EPS, siderophores, and solubilisation of phosphate. Among them, five isolates were selected as plant growth-promoting rhizobacteria (PGPR) based on their PGP features and identified via 16S rRNA sequencing: Enterococcus mundtii strain INJ1 (OR122486), Lysinibacillus fusiformis strain INJ2 (OR122488), Lysinibacillus sphaericus strain MIIA20 (OR122490), Pseudomonas qingdaonensis strain BD1 (OR122487), and Pseudomonas qingdaonensis strain MIA20 (OR122489), all documented in NCBI GenBank. BD1 demonstrated a higher production of superoxide dismutase (SOD) (17.93 U/mg mL), catalase (CAT) (91.17 U/mg mL), and glutathione (GSH) (0.18 U/mg mL), along with higher concentrations of IAA (31.69 µg/mL) and salicylic acid (SA) (14.08 ng/mL). These isolates also produced significant quantities of amino and organic acids. BD1 exhibited superior PGP traits compared to other isolates. Furthermore, the NaCl tolerance of these bacterial isolates was assessed by measuring their growth at concentrations ranging from 0 to 200 mM at 8-h intervals. Optical density (OD) measurements indicated that BD1 and INJ2 displayed significant tolerance to salt stress. The utilisation of these isolates, which enhances plant growth and PGP traits under salt stress, may improve plant development under saline conditions.

Optimizing Productivity and Economics of Maize + Legumes Intercropping: A Study on the Impact of Natural and Organic Farming Practices

Background: Excessive use of chemical fertilizers in a cropping system adversely affects the soil health and crop productivity. To ensure food security, nutritional balance and environmental sustainability, a shift towards eco-friendly organic farming based agricultural systems was imperative. But it also poses challenges for small farmers who owe a small number of animals and they need to purchase bulk organic manures. Zero Budget Natural Farming (ZBNF) emerges as an alternate and advocate for low-input, climate-resilient practices which include beejamrit, jeevamrit, achhadana and whapasa. With the aim of increasing the production of the crops per unit area in the present study, different intervals (14, 21 and 28 days) of jeevamrit under natural farming plots and matka khad (30 days interval) under organic farming plots were tested in different maize based intercropping systems. Methods: A field experiment was conducted during kharif 2020 and 2021 at the Zero Budget Natural Farm (ZBNF), Department of Organic Agriculture and Natural Farming, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur. The experiment was comprised of thirteen treatments which were tested in randomized block design with three replications. Result: Among different treatments significantly higher values of yield attributes and yield of maize were recorded under maize + lobia and jeevamrit spray at 14 days interval during kharif 2020 and maize + soybean and jeevamrit spray at 14 days interval during kharif 2021. In case of economics, higher gross returns, net returns and benefit cost ratio were recorded under maize + soybean and jeevamrit spray at 14 days interval during kharif 2020, whereas during kharif 2021, maize + soybean and matka khad spray at 30 days interval resulted in the highest returns.

Short‐term effects of food waste composts on physicochemical soil quality and horticultural crop production

AbstractAimComposts made from food waste will soon become more widespread on the market thanks to the upcoming enforcement of the legal obligation to sort biowaste. Our experiment aims at improving knowledge on the short‐term effects of these composts on soils’ physicochemical properties and vegetable crops.MethodsThree composts with contrasting characteristics were tested: a 100% v/v green waste compost (C1), and two composts composed of 50% v/v food waste and 50% v/v green waste, one prepared directly on the soil (C2) and the other from a competing producer who have the French NFU 44‐051 (AFNOR NF U 44‐051, 2006) certification for an organic amendment (C3). They were applied at a rate of 100 t ha−1 (dry matter) on two cropped soils with contrasting textures. Soil‐and‐compost mixes and compost‐free soil were planted with lettuce, radish, and potato.ResultsSeventy‐four days after planting, composts improved some soil physicochemical properties. The compost‐amended soils had better saturated hydraulic conductivity (Ks, 1 10−3–2.5 10−3 cm s−1) than the compost‐free soil (0.5 10−3 cm s−1), and water‐stable aggregates were higher than the initial value in C3 soil, equal to it in C2 soil, and lower in C1 soil. pH, total nitrogen, and organic carbon increased in all compost‐amended soils. Food waste compost stimulated crop production. The yields (dry matter) of all three crops were two to three times higher in the two soils amended with food waste compost compared to unamended soil, whereas they decreased almost two times in the soil amended with green waste compost due to nitrogen immobilization. Trace metals (particularly Pb and Cd) added by the composts, although present in edible parts of the plants, did not exceed the European rules for trace metals.ConclusionThus, food waste composts have positive effects on soils and vegetable crops, and the higher their organic matter content, the higher these positive effects.

Impacts of long-term different fertilization regimes on microbial utilization of straw-derived carbon in greenhouse vegetable soils: insights from its ecophysiological roles and temperature responses

As the largest organic carbon input in the agroecosystems, crop residues can increase soil carbon sequestration and crop production in greenhouse vegetable fields (GVFs). However, the soil microbiological mechanisms driving straw decomposition in GVFs under different incubation temperatures and fertilization treatments are not clear. Thus, soil samples were collected from a long-term field experiment included chemical fertilizer application alone (CF), 2/4 fertilizer N+2/4 organic fertilizer N (CM), 2/4 fertilizer N+1/4 organic fertilizer N+1/4 straw N (CMS), 2/4 fertilizer N+2/4 straw N (CS), and incubated with 13C-labeled straw at different temperatures (15, 25, and 35°C) for 60 days. Organic-amended treatments (CM, CMS, and CS), especially CMS treatment, increased soil bacterial Alpha diversity before and after straw addition. Straw decomposition process was dominated by soil Proteobacteria, Actinobacteria, and Firmicutes for each treatments. The effect of incubation temperature on soil microbial community composition was higher than that of fertilization treatments. Soil Alphaproteobacteria and Actinomycetia were the most predominant class involved in straw decomposition. Gammaproteobacteria (Pseudomonas, Steroidobacter, Acidibacter, and Arenimonas) were the unique and predominant class involved in straw decomposition at medium and high temperatures as well as in the straw-amended treatments. Organic-amended treatments, especially straw-amended treatments, increased the relative abundance of glycosyl transferases (GT) and auxiliary activities (AA). Alphaproteobacteria, Actinomycetia, and Gammaproteobacteria had higher relative contribution to carbohydrase genes. In summary, the long-term organic-amended treatments altered the structure of soil microbial communities and increased soil bacterial diversity, with the CMS having a greater potential to enhance resistance to external environmental changes. Soil Alphaproteobacteria and Actinomycetia were responsible for the dominance of straw decomposition, and Gammaproteobacteria may be responsible for the acceleration of straw decomposition. Fertilization treatments promote straw decomposition by increasing the abundance of indicator bacterial groups involved in straw decomposition, which is important for isolating key microbial species involved in straw decomposition under global warming.

A distributed simulation-optimization framework for many-objective water resources allocation in canal-well combined irrigation district under diverse supply and demand scenarios

To address the issues of both water resources allocation and sustainable management in agriculture areas with rising food demand, a simulation-optimization framework based on Flopy and Pymoo was proposed and developed for canal-well combined irrigation districts. The proposed framework first solved the many-objective water resources allocation problem which integrates groundwater simulation, crop production, and farmer income modules to quantitatively reveal the various trade-offs and synergies by using NSGA-III algorithm. The Entropy-TOPSIS method was then applied to recommend proper water allocation schemes. The proposed framework was further tested in Baojixia irrigation district considering various water supply and crop demand scenarios based on Copula-based uncertainty analysis. The Key findings are as follows: (1) the proposed framework could effectively optimize conjunctive water resources allocation problems of both surface water and groundwater; (2) low supply combined with high demand (p=0.17) is more likely to occur than high supply with high demand (p=0.02); (3) increased crop demand and restricted surface water negatively impact both water productivity and groundwater sustainability; and (4) the cumulative groundwater drawdown of recommend schemes is 36.9 % and 6.5 % higher under low to medium supply scenarios, while water productivity of recommend schemes decreases 28.2 % and 9.7 % with high and medium demand. This framework could provide useful insights for sustainable agricultural water management in canal-well combined irrigation district with various uncertainties in supply and demand scenarios.

The crop mined phosphorus nutrition via modifying root traits and rhizosphere micro‐food web to meet the increased growth demand under elevated CO2

AbstractElevated CO2 (eCO2) stimulates productivity and nutrient demand of crops. Thus, comprehensively understanding the crop phosphorus (P) acquisition strategy is critical for sustaining agriculture to combat climate changes. Here, wheat (Triticum aestivum L) was planted in field in the eCO2 (550 µmol mol−1) and ambient CO2 (aCO2, 415 µmol mol−1) environments. We assessed the soil P fractions, root morphological and physiological traits and multitrophic microbiota [including arbuscular mycorrhizal fungi (AMF), alkaline phosphomonoesterase (ALP)‐producing bacteria, protozoa, and bacterivorous and fungivorous nematodes] in the rhizosphere and their trophic interactions at jointing stage of wheat. Compared with aCO2, significant 20.2% higher shoot biomass and 26.8% total P accumulation of wheat occurred under eCO2. The eCO2 promoted wheat root length and AMF hyphal biomass, and increased the concentration of organic acid anions and the ALP activity, which was accompanied by significant decreases in calcium‐bound inorganic P (Ca‐Pi) (by 16.7%) and moderately labile organic P (by 26.5%) and an increase in available P (by 14.4%) in the rhizosphere soil. The eCO2 also increased the growth of ALP‐producing bacteria, protozoa, and bacterivorous and fungivorous nematodes in the rhizosphere, governed their diversity and community composition. In addition, the eCO2 strengthened the trophic interactions of microbiota in rhizosphere; specifically, the eCO2 promoted the associations between protozoa and ALP‐producing bacteria, between protozoa and AMF, whereas decreased the associations between ALP‐producing bacteria and nematodes. Our findings highlighted the important role of root traits and multitrophic interactions among microbiota in modulating crop P‐acquisition strategies, which could advance our understanding about optimal P management in agriculture systems under global climate changes.

Effects of salt stress on the fruit and yield quality of some selected tomato (&lt;i&gt;Lycopersicum esculentum&lt;/i&gt; L.) varieties.

The growth, production, and nutritional characteristics of crops are impacted by abiotic factors such as salinity stress. The study aimed to assess the impact of salt stress on the fruit and yield characteristics of three distinct tomato cultivars, namely Rio, Dan syria, and UTC. Salt solutions with varying concentrations of NaCl were created by dissolving NaCl in deionized water. Four treatments were established, consisting of solutions with salt concentrations of 0g/L (control, without salt), 0.5g/L, 1.0g/L, and 1.5g/L. Each treatment was reproduced three times. The NaCl solutions were administered with a frequency of 7 days. The data in the study underwent Analysis of Variance (ANOVA). Rio outperformed the other varieties in terms of fruit quantity, fresh fruit weight, and fruit pericarp thickness under high salt stress, closely followed by UTC variety. The study also found that Dan syria performed better than the other varieties in terms of fresh and dry weight of shoot, as well as fresh weight of root, under mild and extreme salinity stress. However, Rio performed better than the other varieties in terms of dry weight of root under both mild and extreme salinity stress. The salt tolerance index of the Rio, UTC, and Dan Syria varieties under extreme salinity stress are 46.79%, 20.64%, and 34.70% respectively. This indicated that the Rio variety is the most salt-tolerant of the three types, with Dan Syria variety coming close.

Comparative Metabolome and Transcriptome Analyses Reveal Molecular Mechanisms Involved in the Responses of Two Carex rigescens Varieties to Salt Stress

Salt stress severely inhibits crop growth and production. The native turfgrass species Carex rigescens in northern China, exhibits extraordinary tolerance to multiple abiotic stresses. However, little is known about its specific metabolites and pathways under salt stress. To explore the molecular metabolic mechanisms under salt stress, we conducted metabolome analysis combined with transcriptome analysis of two varieties of Carex rigescens with differing salt tolerances: salt-sensitive Lvping NO.1 and salt-tolerant Lvping NO.2. After 5 days of salt treatment, 114 and 131 differentially abundant metabolites (DAMs) were found in Lvping NO.1 and Lvping NO.2, respectively. Among them, six amino acids involved in the amino acid biosynthesis pathway, namely, valine, phenylalanine, isoleucine, tryptophan, threonine, and serine, were accumulated after treatment. Furthermore, most DAMs related to phenylalanine biosynthesis, metabolism, and phenylpropanoid biosynthesis increased under salt stress in both varieties. The expression profiles of metabolism-associated genes were consistent with the metabolic profiles. However, genes including HCT, β-glucosidases, and F5H, and metabolite 4-hydroxycinnamic acid, of the two varieties may account for the differences in salt tolerance. Our study provides new insights into the mechanisms underlying salt tolerance in Carex rigescens and reveals potential metabolites and genes to improve crop resilience to saline environments.

Quantifying the rainfall variability effects on crop growth and production in the intensified annual forage - winter wheat rotation systems in a semiarid region of China

Replacing summer fallow period (July to September, SF) with annual short-season forages in the traditional fallow-winter wheat (Triticum aestivum L.) system may maintain grain yield and improve productivity in the semi-arid environments. But the uneven and variability rainfall led to instable productivity of the annual forage–winter wheat cropping system. The aims of this study were to 1) quantifying rainfall variability effects on annual forage–winter wheat system crop growing process and productivity; 2) determine the optimal annual forage–winter wheat production system that will response better to future climate change. A four-year (2016–2020) field experiment was conducted to investigate the impact of replacing summer fallow period with annual forages including oat (FO, Avena sativa L.), soybean (SB, Glycine max L.), and vetch (FV, Vicia sativa L.) on plant height (H), leaf area index (LAI), and above-ground biomass (AByield) growth index dynamics under three different levels of rainfall manipulation i.e. 30 % of ambient rainfall exclusion (R-30 %), natural rainfall (CK), and 30 % of ambient rainfall increase (R+30 %). Additionally, we assessed the correlations between forage and winter wheat production with growing season precipitation across 12 rainfall scenarios. Average forage biomass values of oat, soybean, and vetch were 5.50, 4.29, and 2.82 t ha−1, respectively during summer fallow period. The average winter wheat grain yield values in SF, FO, SB, and FV were 3.78, 3.12, 4.02, and 3.18 t ha−1, respectively. Integrating oat into fallow period had negative effects on wheat growth and production, and the H, LAI, and AByield for FO were 63.7 %, 50.9 %, and 29.9 % lower than SF in dry year, but the wheat grain yield in SB were 18.2 % and 24.8 % greater than SF in normal and wet years. Across the four growing seasons, the forage and wheat yields were shown to be strongly related to precipitation, and increasing precipitation significantly enhanced the production. In 2016–2017 growing season, LAI of wheat in SF, FO, SB, and FV with R+30 % scenario was increased by 30.2 %, 21.7 %, 32.7 %, and 19.8 % and that with R-30 % scenario decreased by 23.2 %, 17.8 %, 24.7 %, 16.5 % compared CK, respectively. The traditional summer fallow practice had advantage for maintaining stability in wheat gain production, especially under dry years. In consideration of forage and wheat production to rainfall variability, integrating soybean into fallow season may be an efficient option to maintain wheat yield and produce high forage amount under future climate change on the Loess Plateau and similar semi-arid regions.

Hydroponic Lettuce Cultivation with Organic Liquid Fertilizer: Examining Bacterial Inhibition and Phosphate Solubilization

This study explores organic hydroponic cultivation as a sustainable alternative to chemical fertilizers amid global supply challenges. With rising costs and the depletion of conventional nitrogen sources, organic liquid fertilizers are gaining attention for their microbial enrichment processes (MEP) that convert organic matter into plant-accessible nutrients. This experiment focuses on lettuce cultivation using two organic liquid fertilizers, Power Fish and POF vol. 2, in controlled environments. The results show that there are significant differences in plant growth parameters such as leaf number, fresh weight, chlorophyll content, and root length across different fertilizer treatments. Key findings include that effective management practices, including pH control and regular nutrient application, are crucial for maintaining microbial activity and optimizing nutrient uptake efficiency. Additionally, Bacillus sp. and Xanthomonas sp. were isolated from these fertilizers and dual-cultured with rot fungi, Pythium sp. and Fusarium sp. This dual cultivation demonstrated inhibitory activity against these pathogens, showcasing the potential of these microorganisms in promoting biofilm-mediated disease resistance in organic hydroponic systems. The study also emphasizes the phosphate solubilization capabilities of isolated bacteria, essential for sustainable nutrient cycling. Overall, organic hydroponic systems present a promising strategy for sustainable agriculture, reducing dependency on chemical inputs while enhancing crop productivity and resilience to environmental stressors.

Sugar transporter modulates nitrogen-determined tillering and yield formation in rice

Nitrogen (N) fertilizer application ensures crop production and food security worldwide. N-controlled boosting of shoot branching that is also referred as tillering can improve planting density for increasing grain yield of cereals. Here, we report that Sugar Transporter Protein 28 (OsSTP28) as a key regulator of N-responsive tillering and yield formation in rice. N supply inhibits the expression of OsSTP28, resulting in glucose accumulation in the apoplast of tiller buds, which in turn suppresses the expression of a transcriptional inhibitor ORYZA SATIVA HOMEOBOX 15 (OSH15) via an epigenetic mechanism to activate gibberellin 2-oxidases (GA2oxs)-facilitated gibberellin catabolism in shoot base. Thereby, OsSTP28-OSH15-GA2oxs module reduces the level of bioactive gibberellin in shoot base upon increased N supply, and consequently promotes tillering and grain yield. Moreover, we identify an elite allele of OsSTP28 that can effectively promote N-responsive tillering and yield formation, thus representing a valuable breeding target of N use efficiency improvement for agricultural sustainability.