Crops In sub-Saharan Africa Research Articles

Using cross-country datasets for association mapping in Arachis hypogaea L.

Groundnut (Arachis hypogaea L.) is one of the most important climate-resilient oil crops in sub-Saharan Africa. There is a significant yield gap for groundnut in Africa because of poor soil fertility, low agricultural inputs, biotic and abiotic stresses. Cross-country evaluations of promising breeding lines can facilitate the varietal development process. The objective of our study was to characterize popular test environments in Uganda (Serere and Nakabango) and Malawi (Chitala and Chitedze) and identify genotypes with stable superior yields for potential future release. Phenotypic data were generated for 192 breeding lines for yield-related traits, while genotypic data were generated using skim-sequencing. We observed significant variation (p<0.001; p<0.01; p<0.05) across genotypes for all yield-related traits: days to flowering (DTF), pod yield (PY), shelling percentage, 100-seed weight, and grain yield within and across locations. Nakabango, Chitedze, and Serere were clustered as one mega-environment with the top five most stable genotypes being ICGV-SM 01709, ICGV-SM 15575, ICGV-SM 90704, ICGV-SM 15576, and ICGV-SM 03710, all Virginia types. Population structure analysis clustered the genotypes in three distinct groups based on market classes. Eight and four marker-trait associations (MTAs) were recorded for DTF and PY, respectively. One of the MTAs for DTF was co-localized within an uncharacterized protein on chromosome 13, while another one (TRv2Chr.11_3476885) was consistent across the two countries. Future studies will need to further characterize the candidate genes as well as confirm the stability of superior genotypes across seasons before recommending them for release.

Improving Okra Production by Poultry Manure Application in Highland Acid Oxisols of Dschang, Western Cameroon

Problem Statement: Low yields of crops in Sub-saharan Africa are often associated with low soil fertility. However, due to high cost and negative environmental impact of chemical fertilizers, using cheap and readily available organic manures such as poultry manure (PM) has become indispensable. Aim: To evaluate the effect of different rates of PM on soil fertility and the performance of Okra (Abelmoschus esculentus) in the Cameroon Western Highlands. Methodology: The study was carried out in the field and in the laboratory. The experimental plot (191.25 m2) in the field was designed in a randomized complete block design with six treatments and three replications: 0 t ha-1 of PM (To), 3 t ha-1 of PM (T1), 6 t ha-1 of PM (T2), 9 t ha-1 of PM (T3), 12 t ha-1 of PM (T4), and 250 kg of NPK 12-14-19 (T5). Soil samples were analyzed in the laboratory by standard procedures before and after treatment. Results: Treatment T0 had a clay loam texture, acidic pH (5.4), relatively high organic carbon content (1.92%), moderate total nitrogen (0.33%) and moderate available phosphorus (36.07 mg kg-1). The exchangeable complex revealed high K+ (1.02 cmol kg-1), low Ca2+ (2.60 cmol kg-1) and Mg2+ (1.04 cmol kg-1), average Na+ (0.33 cmol kg-1). After treatment, soil pHH2O, available phosphorus and exchangeable K, Ca, and Mg increased after harvest whereas Na decreased for all the treatments. The effect of the treatments on growth parameters was such that T3&gt;T4&gt;T1&gt;T5&gt;T2&gt;T0. The global trend of yield parameters was such that T3&gt;T4&gt;T5&gt;T1&gt;T0&gt;T2. Economically, treatments T3, T4, and T5 were profitable and recommendable for popularization, with a benefit-to-cost-ratio (BCR) &gt;2. The most economically viable treatment was T3 with a profit rate (PR) of 601.66% and a BCR value of 7.02, while T2 was the least economically viable treatment with a negative PR of -32.14% and a BCR of -33.67. Conclusion: Farmers in Western highlands can produce okra profitably and sustainably using PM at a rate of 9 tha-1f.

Bambara groundnut as a food, nutritional and income security crop in SubSaharan Africa

An over-reliance on the three major global food staples; maize, wheat, and rice, to continue feeding the world is a dangerous approach. Bambara groundnut originated in West-Africa, but was adopted in many African countries, and is produced almost exclusively by small-scale farmers. It has good levels of nutrients, particularly protein, carbohydrates and fat, as well as lysine, in such a balanced manner that it has been termed a “complete food”. The long duration of cooking (hard-to-cook) of bambara groundnut, and the low number of uses in food preparation (in contrast to groundnut) are also factors responsible for lower levels of production and utilization compared to other legumes. Bambara groundnuts have more than 20% protein and can be used as a meat supplement or replacer. It also has high levels of iron and zinc. Landraces with dark-coloured seeds such as red or black, generally contain higher levels of nutrients and minerals than the cream-white or plain-white coloured landraces. The iron content of red bambara seeds was found to be double that of cream-coloured seeds. Antinutrients such as phytic acid can reduce the bioavailability of iron and zinc, but dehulling of the seed can reduce antinutrients significantly. Bambara groundnut has significant potential as a food security crop, but concerted efforts should be made by governments to market the crop, and to develop products and new uses for the seed and the plant. Plant breeding efforts can improve yield and cooking quality. This paper presents progress on the industrial application for food and nutritional security and genetic improvement of bambara groundnut through breeding.

Evaluating Mechanisms of Soil Microbiome Suppression of Striga Infection in Sorghum.

The root parasitic weed Striga hermonthica has a devastating effect on sorghum and other cereal crops in Sub-Saharan Africa. Available Striga management strategies are rarely sufficient or not widely accessible or affordable. Identification of soil- or plant-associated microorganisms that interfere in the Striga infection cycle holds potential for development of complementary biological control measures. Such inoculants should be preferably based on microbes native to the regions of their application. We developed a method to assess microbiome-based soil suppressiveness to Striga with a minimal amount of field-collected soil. We previously used this method to identify the mechanisms of microbe-mediated suppression of Striga infection and to test individual microbial strains. Here, we present protocols to assess the functional potential of the soil microbiome and individual bacterial taxa that adversely affect Striga parasitism in sorghum via three major known suppression mechanisms. These methods can be further extended to other Striga hosts and other root parasitic weeds. Key features • This protocol provides a detailed description of the methods used in Kawa et al. [1]. • This protocol is optimized to assess soil suppressiveness to Striga infection by using natural field-collected soil and the same soil sterilized by gamma-radiation. • This protocol is optimized to test bacterial (and not fungal) isolates. • This protocol can be easily extended to other host-parasite-microbiome systems.

African Cultivated, Wild and Weedy Rice (Oryza spp.): Anticipating Further Genomic Studies.

Rice is a staple crop in sub-Saharan Africa, and it is mostly produced by Asian cultivars of Oryza sativa that were introduced to the continent around the fifteenth or sixteenth century. O. glaberrima, the native African rice, has also been planted due to its valuable traits of insect and drought tolerance. Due to competition and resistance evolution, weedy rice has evolved from O. sativa and O. glaberrima, posing an increasing threat to rice production. This paper provides an overview of current knowledge on the introduction and domestication history of cultivated rice in Africa, as well as the genetic properties of African weedy rice that invades paddy fields. Recent developments in genome sequencing have made it possible to uncover findings about O. glaberrima's population structure, stress resilience genes, and domestication bottleneck. Future rice genomic research in Africa should prioritize producing more high-quality reference genomes, quantifying the impact of crop-wild hybridization, elucidating weed adaptation mechanisms through resequencing, and establishing a connection between genomic variation and stress tolerance phenotypes to accelerate breeding efforts.

Maize yields have stagnated in sub-Sahara Africa: a possible transgenic solution to weed, pathogen and insect constraints.

Despite major breeding efforts by various national and international agencies, yields for the ~40 million hectares of maize, the major food crop in sub-Saharan Africa, have stagnated at <2 tons/ha/year for the past decade, one-third the global average. Breeders have succeeded in breeding increased yield with a modicum of tolerance to some single-weed or pathogen stresses. There has been minimal adoption of these varieties because introgressing polygenic yield and tolerance traits into locally adapted material is very challenging. Multiple traits to deal with pests (weeds, pathogens, and insects) are needed for farmer acceptance, because African fields typically encounter multiple pest constraints. Also, maize has no inherent resistance to some of these pest constraints, rendering them intractable to traditional breeding. The proposed solution is to simultaneously engineer multiple traits into one genetic locus. The dominantly inherited multi-pest resistance trait single locus can be bred simply into locally adapted, elite high-yielding material, and would be valuable for farmers, vastly increasing maize yields, and allowing for more than regional maize sufficiency. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Plant-parasitic nematodes associated with maize, and their relationship with soil properties in southern and southwestern Ethiopia

Summary Maize is an important cereal crop in sub-Saharan Africa (SSA). Plant-parasitic nematodes significantly impact maize production. However, research on maize nematodes in SSA is limited. The current study aimed to assess the occurrences and densities of nematodes associated with maize and to assess the correlation of some soil physicochemical properties with nematodes in southern and southwestern Ethiopia. One hundred and eighty-six soil and root samples were collected from 62 maize fields (31 from Hawassa Zuria and 31 from Mana districts). Eleven plant-parasitic nematode genera were identified. The most important genera of plant-parasitic nematodes identified were Meloidogyne and Pratylenchus, followed by Helicotylenchus and Tylenchorhynchus. The nematodes Pratylenchus and Criconemoides were significantly and positively associated with pH, soil organic carbon (SOC) and soil organic matter (SOM). Additionally, Tylenchorhynchus, Pratylenchus and Rotylenchulus were significantly positively correlated with soil available phosphorus (SAP). However, Criconemoides and Meloidogyne had a strong negative correlation with soil bulk density (SBD). This study provides useful baseline information on maize-associated nematodes, and will be useful for agricultural policy makers, private farmers, non-governmental organisations and agricultural extension workers enabling them to establish sustainable maize nematode management strategies.

Farming practices, varietal preferences, and land suitability analyses for yam production in Eastern D.R. Congo: implications for breeding initiatives and food sovereignty

Yam (Dioscorea spp.) is a key tuber crop in sub-Saharan Africa (SSA) with huge potential for poverty alleviation, food sovereignty, and nutrition security. Exploiting its full potential requires that factors holding it down are understood and mitigated. This study, conducted between May and July 2022 and 2023, assessed yam farming practices, varietal preferences, and land suitability in the Eastern Democratic Republic of Congo (DRC). We interviewed 765 smallholder farmers within four agro-ecological zones (AEZ) to assess the sociocultural, agronomic, varietal, and biophysical factors affecting yam production. Land suitability analyses were conducted using the Analytic Hierarchy Process (AHP) to identify areas suitable to extensive yam production and to cluster environments that could optimize yam varietal selection and testing. Results showed that yam is mainly cultivated by women in eastern DRC (70%). The seed delivery system is informal, relying primarily on farmer-saved seeds and farmer-seed exchanges (74.9%). Soil depletion (68.3%), limited access to high-quality seeds (54.5%), youth disengagement in yam value chain (50.3%), insect pests (17.9%), and short tuber shelf-life (65.8%) were, respectively, the main ecological, agronomic, sociocultural, biological, and tuber quality factors hindering yam production in eastern DRC. However, the perceived importance of most factors significantly varied with farmer gender and age categories. A multitude of varietal traits was used to assess yam varieties in eastern DRC, of which the tuber taste (59%) was the most valued trait regardless of gender and age categories, though it had highest scores among middle-aged adult women. Land suitability analyses discriminated five clusters; the most significant part of the region falling under suitable (27%), highly suitable (24%), and very highly suitable classes (37%). We further discussed how breeding initiatives for delivering yam varieties, suiting local producers’ and end-users’ needs, could unlock the crop’s potential for enhancing food security and wealth creation in eastern DRC. The land suitability map from this study is a valuable decision-making tool in defining priority areas for extensive yam production and varietal selection and testing. This study provided valuable insights on factors affecting yam production and suggested yam breeding as a backbone of a holistic approach seeking to address challenges faced in unlocking the potential for yam production in eastern DRC.

Mehlich 3 as an indicator of grain nutrient concentration for five cereals in sub-Saharan Africa

Context or ProblemSoil testing for available nutrients is an important tool to determine fertilizer rates, however many standard methods test the availability of a single nutrient only. In contrast, Mehlich 3 (M3) is a multi-element test for predicting crop yield responses to the addition of macro and micronutrients. However, the M3 test has rarely been validated against crop nutrient concentrations, which limits its application for dietary improvement studies in sub-Saharan Africa. Objective or Research QuestionThe primary objective was to test how well the M3 nutrient concentrations corresponds to grain nutrient concentrations as an indicator of plant nutrient status and grain quality. A secondary objective was to compare the performance of the M3 test with other extraction tests. MethodsThis study used 1096 paired soil and crop samples of five cereals: maize, rice, sorghum, teff and wheat, covering a broad range of soil types and soil properties in Ethiopia and Malawi (e.g., pH 4.5 - 8.8; Olsen P < 1 - 280 ppm). The samples were selected from a larger collection based on “high” or “low” grain nutrient concentrations in the crop, and the respective soil available nutrients were measured with M3 and other extraction tests: CaCl2 (P, K, Mg, Mn), Ca(NO3)2 (K and Mg), Olsen P, sequential extraction (S), and DTPA (Mn, Fe and Zn). ResultsThe M3 concentrations followed the trend of the “high” and “low” grain concentrations in nearly all nutrients and crops, and this was statistically significant in teff and wheat for all nutrients. The results were best for macronutrients, and slightly less good for micronutrients, probably because the concentration of micronutrients in the selected soil samples was generally quite low. Compared to the other multi-element extractant (CaCl2), the M3 test corresponded better to grain concentrations of K and Mg, and equally well to Olsen P, sequential extraction (S), and DTPA predictions of P, S, Zn and Fe, respectively. M3 extracted much greater concentrations than the other tests, and this was more pronounced in alkaline soils. ConclusionsGiven that the M3 test corresponded well to grain nutrient concentrations across a range of soils and crops in sub-Saharan Africa (SSA), we conclude that it can be considered a universal test for plant nutrients. We also proposed thresholds for M3 values, defining below optimum, optimum and above optimum soil fertility status. Implications or SignificanceThese results validate the use of the M3 test to assess soil fertility and develop fertilizer recommendations for improved produce quality to enhance diets in SSA.

Economical Assessment of NSPRI Parabolic Shaped Solar Dryer for Cowpea Flour Production

Cowpea (Vigna unguiculata) is a crucial staple crop in sub-Saharan Africa, providing essential nutrients but facing challenges in storage and processing. This study presents an economic assessment of the NSPRI Parabolic Shaped Solar Dryer (PSSD) for cowpea flour production, focusing on its potential impact on food processing and economic development. Cowpea, a vital legume in sub-Saharan Africa, offers nutritional benefits but faces challenges in storage and processing due to traditional drying methods. The research explores the utilization of solar drying technology to enhance cowpea preservation and value addition. The study area, Stop Hunger Women Cooperative Investment and Credit Society Limited in Ibadan, Nigeria, served as the research site. The NSPRI PSSD, with its innovative design optimized for solar radiation absorption, provided an efficient drying environment for cowpeas. Economic analysis, including Return on Investment (ROI) of 64%, Benefit-Cost Ratio (BCR) of 1.64, and Payback Period (PBP) of approximately one year and seven months, was determined to assess the viability of bean flour production using the solar dryer. The study underscores the economic viability of adopting solar drying technology for food processing, offering benefits such as reduced post-harvest losses, improved food quality, and potential employment generation. These findings provide valuable insights for stakeholders seeking sustainable solutions to enhance food security and economic growth in agricultural communities.

Recent advances in the population biology and management of maize foliar fungal pathogens Exserohilum turcicum, Cercospora zeina and Bipolaris maydis in Africa.

Maize is the most widely cultivated and major security crop in sub-Saharan Africa. Three foliar diseases threaten maize production on the continent, namely northern leaf blight, gray leaf spot, and southern corn leaf blight. These are caused by the fungi Exserohilum turcicum, Cercospora zeina, and Bipolaris maydis, respectively. Yield losses of more than 10% can occur if these pathogens are diagnosed inaccurately or managed ineffectively. Here, we review recent advances in understanding the population biology and management of the three pathogens, which are present in Africa and thrive under similar environmental conditions during a single growing season. To effectively manage these pathogens, there is an increasing adoption of breeding for resistance at the small-scale level combined with cultural practices. Fungicide usage in African cropping systems is limited due to high costs and avoidance of chemical control. Currently, there is limited knowledge available on the population biology and genetics of these pathogens in Africa. The evolutionary potential of these pathogens to overcome host resistance has not been fully established. There is a need to conduct large-scale sampling of isolates to study their diversity and trace their migration patterns across the continent.

Optimizing seeding rates for irrigated bread wheat (Triticum aestivum L.) varieties in the lowland area of Jawi, northwestern Ethiopia

Bread wheat (Triticum aestivum L.) is one of the main food security crops in sub-Saharan Africa. The Ethiopian government is putting a great effort into increasing the production of bread wheat under various production systems to narrow the gap between demand and supply. There is no agronomic recommendation for wheat production produced under irrigation conditions. Thus, this study was initiated to identify the optimum seeding rates of different wheat varieties for the highest-yield and profitability in the lowland area of northwestern Ethiopia under irrigation conditions in 2020/2021. The experiment was laid out using a randomized complete block design with three replications. The treatments are five levels of seeding rates (100, 125, 150, 175 and 200 kg ha-1) and three bread wheat varieties (Fentale-2, Kakaba and Ogelcho). Wheat yield and yield component data were statistically subjected to analysis of variance using SAS-JMP-16 software. Results revealed that the performance of wheat varieties in the lowland area of Jawi was promising irrespective of their yield differences. The study showed the highest number of total and effective tillers per unit area; and biomass and grain yield were recorded when varieties Fentale-2, Kakaba and Ogelcho were planted at a seed rate of 200 kg ha-1, 175 kg ha-1 and 200 kg ha-1, respectively.The same varieties gave a grain yield of 3.99 t ha-1, 3.47 t ha-1 and 3.44 t ha-1, respectively. On the other side, maximum net benefit and marginal rate of return were obtained when the variety Fentale-2 was planted at a seeding rate of 200 kg ha-1 (NB = 65,778.8 ETB ha-1 and MRR = 224 %) followed by the same variety at the seed rate 175 kg ha-1 (NB = 63,402.4 ETB ha-1 and MRR = 297 %). Thus, it can be concluded that planting variety Fentale-2 with 200 kg ha-1 seed rate under irrigation condition is the promising agronomic practice in lowland agro-ecology of Jawi area. However, further research is also recommended by considering the appropriate planting time and the irrigation water requirement.

Water sustainability of sub-Saharan African cotton industry: evidence from Mali

The world's cotton industry plays a significant role in the global economy, with cotton being used in around 50% of all clothing, household items, and other products. However, the production of cotton is also known to have a significant impact on the environment, particularly freshwater resources. Sub-Saharan Africa is particularly vulnerable, with 40% of the world's water-deprived people living in the region. Cotton is a vital cash crop in sub-Saharan Africa, serving as the main source of livelihood for over 2 million rural families, but its production is also water-intensive, requiring around 20,000 litters of water per kg of cotton yield. In addition, cotton farming in the region is largely dependent on rain, but countries are looking to expand their production and acreage through increased irrigation, putting further pressure on already stretched water resources. This essay investigates the sustainability of sub-Saharan Africa's cotton industry, using Mali as a case study. The region can increase its output significantly, but this must be done sustainably to avoid exacerbating the negative impact on freshwater resources. The study also discusses the water crisis in sub-Saharan Africa, cotton farming in Mali and the region, and the potential consequences of increased cotton production on families and the environment. Mali, currently the highest cotton producer on the continent is a convenient case study reflecting conditions in other sub-Saharan countries. By studying the scientific literature on the progression of cotton production and attendant problems in Mali and some other West African countries, I hope to inform policymakers in the region about some proven ways to improve cotton yield and processing while leaving water resources pristine. The plan centres on producing cotton in an eco-friendly manner through the adoption of organic cotton, and GM cotton and irrigation in suitable areas. At the same time, there is a need to reduce the grey water footprint by reducing chemical usage and treating effluents before discharge.

The potential of provitamin A maize ( Zea mays L.) biofortification in sub-Saharan Africa: Current status, opportunities, challenges and perspectives: A review

Abstract Maize is a potential crop for improving food and nutritional security for over 300 million people in sub-Saharan Africa (SSA). It is the most preferred cereal crop in SSA because of its diversified uses, taste and higher grain yield potential compared to other cereals. However, overdependence on maize-based diets without vitamin A supplementation is associated with the highest rates of vitamin A deficiency (VAD) in SSA. Vitamin A deficiency mostly affects children under the age of 5 years, pregnant and lactating women, accounting for the worst disease burden, and infant mortality in SSA. To date provitamin A maize breeding programmes of HarvestPlus partnered with CIMMYT and IITA, private seed companies and national plant breeding programmes developed at least 70 elite varieties to curb VAD in SSA. This review summarizes the potential of provitamin A maize in SSA focusing on (1) reasons why maize is a good choice for provitamin A biofortification, (2) the prevalence of vitamin A deficiency in SSA, (3) the Global Hunger Index (GHI) and extent of hidden hunger, (4) possible interventions to curb vitamin A deficiency in SSA and their applicability, (5) status of the provitamin A maize varieties released, (6) level of adoption and consumer perception of provitamin A maize-based food, (7) the impact of climate change in maize productivity in SSA and (8) the breeding methods for developing provitamin A maize-resilient genotypes to mitigate the effects of climate change in SSA.

Hydroxamic acids: New players in the multifactorial mechanisms of maize resistance to Striga hermonthica

Striga hermonthica is the most widespread and destructive plant parasite infesting maize and other major crops in sub-Saharan Africa where it causes severe yield losses and threatens food security. Several tolerant maize lines supporting reduced S. hermonthica emergence have been deployed. However, the molecular bases of such resistance are yet poorly understood. Based on a time course comparative gene expression analysis between susceptible and resistant maize lines we have confirmed resistance mechanisms known to be activated upon plant parasite infestation and identified potential novel players worth further investigation e.g. iron homeostasis and mitochondrial respiration-related genes. Most intriguingly, we show a previously unknown strategy of maize post-attachment resistance based on DIMBOA accumulation in S. hermonthica-infested maize roots. S. hermonthica infestation triggers positive regulation of gene expression in the hydroxamic acid (HA) pathway culminating with an accumulation of benzoxazinoids (BX), known for their antifeedant, insecticidal, antimicrobial, and allelopathic activities. We demonstrate that HA root content is positively correlated with S. hermonthica resistance in the resistant parent and its progenies and in unrelated maize lines. Downregulation of HA genes causes increased susceptibility to S. hermonthica infestation in loss-of-function maize mutants. While the mechanism of BX action in parasitic plant resistance is yet to be uncovered, the potential of this discovery for developing effective control and breeding strategies is enormous.

SEGS-1 a cassava genomic sequence increases the severity of African cassava mosaic virus infection in Arabidopsis thaliana.

Cassava is a major crop in Sub-Saharan Africa, where it is grown primarily by smallholder farmers. Cassava production is constrained by Cassava mosaic disease (CMD), which is caused by a complex of cassava mosaic begomoviruses (CMBs). A previous study showed that SEGS-1 (sequences enhancing geminivirus symptoms), which occurs in the cassava genome and as episomes during viral infection, enhances CMD symptoms and breaks resistance in cassava. We report here that SEGS-1 also increases viral disease severity in Arabidopsis thaliana plants that are co-inoculated with African cassava mosaic virus (ACMV) and SEGS-1 sequences. Viral disease was also enhanced in Arabidopsis plants carrying a SEGS-1 transgene when inoculated with ACMV alone. Unlike cassava, no SEGS-1 episomal DNA was detected in the transgenic Arabidopsis plants during ACMV infection. Studies using Nicotiana tabacum suspension cells showed that co-transfection of SEGS-1 sequences with an ACMV replicon increases viral DNA accumulation in the absence of viral movement. Together, these results demonstrated that SEGS-1 can function in a heterologous host to increase disease severity. Moreover, SEGS-1 is active in a host genomic context, indicating that SEGS-1 episomes are not required for disease enhancement.

Correlate the cyanogenic potential and dry matter content of cassava roots and leaves grown in different environments

Cassava (Manihot esculenta Crantz) is an essential stable food crop in Sub-Saharan Africa commonly consumed amongst the low-income communities in Africa. Though cassava roots and leaf have vast economic and commercial benefits, it produces cyanogenic glycosides, which are toxic and most often responsible for the bitter taste of some cassava cultivars. The study evaluates the cassava roots and leaves’ cyanogenic potential and dry matter content of the Genetic Gain Assessment trial grown in a different environment. It establishes the association between the cyanogenic potential (CNP) and the roots and leaves dry matter (DM). Genetic Gain Assessment (GGA) cassava genotypes (N = 400) selected for the Uniform Yield Trial (UYT) breeding stage were planted under IVS (Dry season in Inland Valley Hydromorphic area) and Upland (rain-fed conditions) in two locations of IITA Research Farms, namely; Ibadan (IVS and Upland) and Mokwa (Upland) in Nigeria. The CNP content of cassava leaves in IVS, Mokwa, and Upland ranged from 3.39 to 272.16 mg/100 g, 4.28 to 228.72 mg/100 g, and 13.13 to 127.39 mg/100 g, respectively. However, the respective CNP range in root samples across IVS, Mokwa, and Upland was 0.76–76.31 mg/100 g, 0.94–136.53 mg/100 g, and 2.37–47.11 mg/100 g. Also, the mean ± SD of DM content of leaves were 27.97 ± 3.01%, 28.81 ± 4.01%, and 13.65 ± 3.69%, respectively, in IVS, Mokwa, and Upland, while the root samples had mean ± SD of DM content of 38.09 ± 4.80%, 32.69 ± ,5.93% and 24.63 ± 5.07% respectively. Furthermore, location and genotype had a highly significant effect (p < 0.001) on the CNP and DM of roots and leaves. Also, linear regressions were established between CNP and DM of root and leaf with regression equation; DM-Root = 1.1999*DM-Leaf (r = 0.956) and CNP-Root = 0.29006*CNP-Leaf (r = 0.54). The relationship between the DM (root and leaf) and CNP (root and leaf) could serve as a valuable “inter-prediction” tool for these parameters.

Gendered perceptions and adaptations to climate change in Ghana: what factors influence the choice of an adaptation strategy?

Climate change poses a significant threat to various sectors, including agriculture, affecting men and women unevenly. Although gender-based perceptions of climate change have been studied, there remains a gap in understanding how these perceptions influence the adoption of adaptation strategies among men and women smallholder farmers in the production of leguminous crops in sub-Saharan Africa. This study investigated the gender differences in the adoption of climate change adaptation strategies among bean and cowpea farmers in Ghana. The findings revealed that socioeconomic and institutional factors significantly influenced the choice of adaptation strategies, with notable differences between men and women. Higher levels of education, farming experience, marital status, access to credit, and education determined the choice of adaptation strategies. On the other hand, women farmers, despite having lower levels of formal education, showed a higher utilization of extension services, possibly due to targeted efforts to reach out to more women farmers. Larger households were less likely to adopt mixed cropping and changing cropping patterns, while married individuals were less likely to use crop rotation. Training and access to credit significantly increased the likelihood of adopting crop rotation, changing cropping patterns, and using improved seeds. The study also found that [f]armers perceptions of the impacts of dry spells and delayed onset of rains influenced the use of climate change adaptation strategies. Furthermore, farmers who participated in climate change planning were more likely to use diverse adaptation strategies, underscoring the importance of a locally focused, inclusive planning process. However, gender differences were observed in the determinants of the use of these strategies. For instance, while access to extension services was found to be more influential for women, men’s decisions were more influenced by their marital status, access to credit, and education. Policy makers and local institutions need to encourage and facilitate farmers’ involvement in climate change planning processes to enable designing of effective, context-relevant, inclusive, and sustainable climate change adaptation strategies. Distinct differences in the factors underlying the use of adaptation strategies by men and women demand creation of and implementation of gender-sensitive programs that effectively reach and benefit both women and women.

Small Molecules with Thiourea Skeleton Induce Ethylene Response in Arabidopsis.

Ethylene is the only gaseous plant hormone that regulates several aspects of plant growth, from seedling morphogenesis to fruit ripening and organ senescence. Ethylene also stimulates the germination of Striga hermonthica, a root parasitic weed that severely damages crops in sub-Saharan Africa. Thus, ethylene response stimulants can be used as weed and crop control agents. Ethylene and ethephon, an ethylene-releasing compound, are currently used as ethylene response inducers. However, since ethylene is a gas, which limits its practical application, we targeted the development of a solid ethylene response inducer that could overcome this disadvantage. We performed chemical screening using Arabidopsis thaliana "triple response" as an indicator of ethylene response. After screening, we selected a compound with a thiourea skeleton and named it ZKT1. We then synthesized various derivatives of ZKT1 and evaluated their ethylene-like activities in Arabidopsis. Some derivatives showed considerably higher activity than ZKT1, and their activity was comparable to that of 1-aminocyclopropane-1-carboxylate. Mode of action analysis using chemical inhibitors and ethylene signaling mutants revealed that ZKT1 derivatives activate the ethylene signaling pathway through interactions with its upstream components. These thiourea derivatives can potentially be potent crop-controlling chemicals.

Examining the contribution of an underutilized food source, Bambara Groundnut, in improving protein intake in Sub-Saharan Africa

IntroductionBambara groundnut (BGN) is an underutilised, indigenous crop in sub-Saharan Africa. As an underutilised crop the nutritional contribution it can make is not well researched and documented. This study aims to better understand the nutritional profile and protein quality of BGN and the potential of this legume to combat food and nutritional security in rural and malnourished communities.MethodsNutritional analysis, including proximate analysis, minerals, total fat and amino acid profile of BGN from three different regions in the Mpumalanga (MP) province of South Africa (SA), were determined. The protein quality was evaluated with various methods including CSEAA, IEAA, PDCAAS and DIAAS and compared with other pulses and legumes in the diet.ResultsBGN consists of - 18,9% carbohydrates, 9,4% protein and 3% fat as consumed. The protein quality is lower than some other commonly consumed pulses but can still be valuable as a complementary protein in the current traditional diet of the region.DiscussionThere is the potential that BGN, together with other commonly consumed plant-based foods (e.g. maize) form a complete protein, that can contribute to protein intake and alleviate the burden of protein-energy malnutrition, specifically in developing countries.