Crop Seeds Research Articles

Optimizing Cereal Crop Performance with Nutrient Priming

The fundamental building blocks for the success of stand establishment for any crop plant are better germination and robust seedlings. Pre-treatment of the seeds is one of the best methods that may possibly improve germination and maintain the uniformity and stability of the seedlings. Primed seeds usually exhibit synchronized and early germination and sometimes greater total germination percentage over a range of environmental conditions. This enhancement has been linked to osmotic correction, metabolic repair during imbibition, and the accumulation of chemicals that promote germination. Nutri-priming is a good alternative to meet plant nutritional requirements. Seed priming with macro or micronutrient (nutripriming) application has been recently done on various agronomic crops including rice, maize and wheat; lead to better germination and establishment. It aims to combine the positive effects of hydro priming with the nutritional aspect of fertilizers. Crop seeds can be primed with either micro or macronutrients, which improves the nutrient content and increases the germination, sprouting, and water uptake efficiency. Primed seeds performed better under diverse environments especially under sub optimal conditions at sowing and resulted in higher yield compared to unprimed seeds. Priming requires specific temperature and time, if it is not followed then the seed can be deteriorated due to embryo protrusion. Due to readily available food during germination, primed seed are better able to complete the process of germination in a short time and cope with environmental stresses including low temperatures. In fact, it may be regarded as a useful method for enhancing crop establishment in regions with unfavourable agro-climatic conditions (rainfed, dry farming, and dry land farming) with increased yield, tolerance to stressful situations, resistance to diseases, crop competitiveness against weeds, and water use efficiency.

Non-Foliar Photosynthesis in Pea (Pisum sativum L.) Plants: Beyond the Leaves to Inside the Seeds

In addition to leaves, photosynthesis can occur in other green plant organs, including developing seeds of many crops. While the majority of studies examining photosynthesis are concentrated on the leaf level, the role of other green tissues in the production of total photoassimilates has been largely overlooked. The present work studies the photosynthetic behavior of leaves and non-foliar (pericarps, coats, and cotyledons) organs of pea (Pisum sativum L.) plants at the middle stage of seed maturation. The Chl a fluorescence transient was examined based on OJIP kinetics using the FluorPen FP 110. A discrepancy was observed between the performance index (PIABS) for foliar and non-foliar plant tissues, with the highest level noted in the leaves. The number of absorbed photons (ABS) and captured energy flow (TRo) per reaction center (RC) were elevated in the non-foliar tissues, which resulted in a faster reduction in QA. Conversely, the energy dissipation flux per RC (DIo/RC and PHI_Do) indicated an increase in the overall dissipation potential of active reaction centers of photosystem II. This phenomenon was attributed to the presence of a higher number of inactive RCs in tissues that had developed under low light intensity. Furthermore, the expression of genes associated with proteins and enzymes that regulate ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) activity was observed, including chaperonins Cpn60α and Cpn60β, RuBisCO activase, as well as phosphoribulokinase. The expression of these genes was found to differ between foliar and non-foliar tissues, indicating that the activation state of RuBisCO may be modified in response to light intensity. Overall, the present study provides insights into the mechanisms by which non-foliar green tissues of plants adapt to efficient light capture and utilization under low light conditions.

MRI-Seed-Wizard: Combining Deep Learning Algorithms with Magnetic Resonance Imaging Enables Advanced Seed Phenotyping.

Evaluation of relevant seed traits is an essential part of most plant breeding and biotechnology programs. There is need for non-destructive, three-dimensional assessment of the morphometry, composition, and internal features of seeds. Here, we introduced a novel tool, MRI-Seed-Wizard, which integrates deep learning algorithms with non-invasive magnetic resonance imaging (MRI) for its use in the new domain - plant MRI. The tool enabled in vivo quantification of 23 grain traits, including volumetric parameters of inner seed structure. Several of these features cannot be assessed using conventional techniques, including X-ray computed tomography. MRI-Seed-Wizard was designed to automate the manual processes of identifying, labeling, and analyzing digital MRI data. We further provide advanced MRI protocols that allow the evaluation of multiple seeds simultaneously to increase throughput. The versatility of MRI-Seed-Wizard in seed phenotyping was demonstrated for wheat (Triticum aestivum) and barley (Hordeum vulgare) grains, and is applicable to a wide range of crop seeds. Thus, artificial intelligence, combined with the most versatile imaging modality - MRI, opens up new perspectives in seed phenotyping and crop improvement.

Effects of priming duration and rhizosphere bacteria metabolite concentration on the germinability of cowpea, soybean, sesame, and okra seeds

Seed priming enhances germination and growth, which are important determinants of crop yield. This study was carried out to assess the effect of priming duration and metabolite concentration on the priming of five (5) crops using the metabolites of five (5) bacterial isolates. The crop seeds were treated in the cold-extracted metabolites of the five isolates at five (5) different priming durations (1, 2, 3, 4, and 5 h) and then in five metabolite concentrations (200, 400, 600, 800, and 1000 mg/L) of the five extracted metabolites at the optimal priming duration determined in the first experiment. Characterization of the cold-extracted metabolites was also carried out using gas-chromatography-mass spectrometry (GC-MS). Results revealed that priming cowpea and soybean seeds for longer durations (< 3 h) could hinder their growth and development. Lower concentrations were observed to be optimal for cowpea and soybean, but for sesame and okra, there was no detectable pattern with metabolite concentration. The GC-MS revealed the presence of some molecules (e.g. hexadecanoic acid) that have shown plant growth promotion potential in other studies. This study showed that seeds with large endosperms, such as those of cowpea and soybean, are more prone to the harmful effects of treatment for longer durations. Further experiments should be undertaken to isolate and purify the bioactive moieties for further studies and application.

The Study of the Process of Supplying Seeds of Row Crops Using a Vacuum Seed-Placing Unit

Introduction. The performance of precision seeders depends on many factors, including the functionality of their dosing systems, the modernization of which is possible only on the basis of patterns, which allows forecasting the indicators of single seed placing into a furrow.Aim of the Study. The study is aimed at analyzing the influence of the diameter of the suction holes of seed-placing units and the rarefaction created in them on the characteristics of placing seeds of the main row crops.Materials and Methods. The results of the study were obtained based on a series of experiments on sowing corn and sunflower seeds using an MS-8 seeder, the operation principle of which can be considered typical for vacuum seed-placing units.Results. There have been obtained empirical dependences of the particular features of zero and group placing seeds of such row crops as corn and sunflower on the area of the suction holes and the rarefaction in the vacuum chamber.Discussion and Conclusion. The quantitative estimates obtained may differ for seeds of the same crop, but having different technological properties. The general factor is that the frequency of missing seeds is proportional to the suction force magnitude in the working plane of the metering element, regardless of its area. At the same time, the probability of double seed placing depends on the area of the metering ports with a constant suction force, a decrease in their diameter leads to a decrease in the frequency of double seed placing. The data obtained allow considering joint variations in the magnitude of the rarefaction in the vacuum chamber and the diameter of the suction holes as effective tools for improving the seed placing quality.

Sesame, an Underutilized Oil Seed Crop: Breeding Achievements and Future Challenges.

Sesame seeds and their edible oil are highly nutritious and rich in mono- and polyunsaturated fatty acids. Bioactive compounds such as sterols, tocopherols, and sesamol provide significant medicinal benefits. The high oil content (50%) and favorable mono- and polyunsaturated fatty acid balance, as well as resilience to water stress, make sesame a promising candidate crop for global agricultural expansion. However, sesame production faces challenges such as low yields, poor response to agricultural inputs, and losses due to capsule dehiscence. To enhance yield, traits like determinate growth, dwarfism, a high harvest index, non-shattering capsules, disease resistance, and photoperiod sensitivity are needed. These traits can be achieved through variation or induced mutation breeding. Crossbreeding methods often result in unwanted genetic changes. The gene editing CRISPR/Cas9 technology has the potential to suppress detrimental alleles and improve the fatty acid profile by inhibiting polyunsaturated fatty acid biosynthesis. Even though sesame is an orphan crop, it has entered the genomic era, with available sequences assisting molecular breeding efforts. This progress aids in associating single-nucleotide polymorphisms (SNPs) and simple sequence repeats (SSR) with key economic traits, as well as identifying genes related to adaptability, oil production, fatty acid synthesis, and photosynthesis. Additionally, transcriptomic research can reveal genes involved in abiotic stress responses and adaptation to diverse climates. The mapping of quantitative trait loci (QTL) can identify loci linked to key traits such as capsule size, seed count per capsule, and capsule number per plant. This article reviews recent advances in sesame breeding, discusses ongoing challenges, and explores potential strategies for future improvement. Hence, integrating advanced genomic tools and breeding strategies provides promising ways to enhance sesame production to meet global demands.

Understanding the Significance of Raffinose Family Oligosaccharides in Seed Physiology

Seed vigour and longevity are pivotal agronomic traits with profound implications for crop yield, food security, and the global economy. Seed longevity, often defined by the duration of seed viability, is crucial for effective gene bank management, influencing seed regeneration cycles and ensuring the long-term conservation of plant genetic resources. The inevitable process of seed deterioration, driven by a complex network of biochemical reactions, leads to altered metabolism and damage to critical cellular components such as membranes, DNA, mitochondria, proteins, and the antioxidative defence system. In recent research, Raffinose family oligosaccharides (RFOs) have emerged as key contributors to enhancing seed vigour and longevity. These sugars perform multiple functions in plants, including tolerance to abiotic and biotic stresses, regulation of seed germination, and maintenance of desiccation tolerance, all of which are essential for overall seed health. Studies suggest that RFOs significantly bolster seed vigour and longevity through mechanisms such as cytoplasmic vitrification, water replacement, and osmoprotection in dried seeds. These oligosaccharides are particularly abundant in the seeds of leguminous crops and are also found in roots and specialized storage organs. The glassy state formed by RFOs is vital for protecting cells from oxidative damage caused by reactive oxygen species (ROS), enhancing the stability of enzymes, and preventing deleterious conformational changes in proteins. Furthermore, delaying the degradation of RFOs has been shown to inhibit premature germination, underscoring their critical role in early seedling development and successful crop establishment.

Finger Millet (<i>Eleusine Coracana</i> (L.) Gaertn) Production Status, Challenges, and Seed Source in Ethiopia; A Review

Similar to other cereal crops finger millet is being produced in a large volume in Ethiopia, as they are the principal staple food crop. Finger millet is considered as underutilized cereal crop which is commonly grown in the northern, west-northern, and western parts of the country and it is considered as a crop which is important to overcome malnutrition. Regions including Tigray, Amhara, Oromiya, Benishangul-Gumuz, Southern Nation and Nationalities Peoples (SNNP) and Gambela are considered as the potential regions for the production of finger millet in Ethiopia. Relatively, it is considered as drought tolerant as compared to other major cereals such as rice, wheat, and barley. In addition to this finger millet is considered as a highly resilient crop which can grow in different weather conditions, including drought, floods, and marginal soils. In Ethiopia it is commonly grown for the preparation of local foods like ‘injera’ and porridge and local drink such as ‘areki’ or ‘tella’. About 26 varieties of finger millet are cultivated in main season in the country. In Ethiopia, there are a limited source of cereal crop seeds which includes, farmer’s own saved seeds and government seed distributing companies. In this review the status of production, seed source and nutritional importance of finger millet in Ethiopia is presented.

Analysis of Supply Chain Management Against Operations of PT Benih Citra Asia

PT Benih Citra Asia is a company that operates in industry in agriculture, in particular industry seeds of food and horticultural crops which are the result of plant breeding (Plant Breeding). This research aims to analyze the effect of implementation of Supply Chain Management (SCM) on the operations of PT Benih Citra Asia. This research uses qualitative methods by conducting in-depth interviews and direct observation as data collection techniques. The research results show that implementation of Supply Chain Management at PT Benih Citra Asia has had a significant positive impact on the company's operational efficiency. Supply Chain Management helps optimize the flow of goods and information throughout the supply chain, which contributes to smooth production processes and responsiveness to market changes. Good collaboration between departments, especially between the Plant and IT divisions, is very important to ensure that product information is always up to date and accurate. Apart from that, this research also found that raw material inventory management that is difficult to predict and fluctuations in customer demand are the main challenges in Supply Chain Management. To overcome this problem, closer collaboration with suppliers and customers is required as well as the use of advanced information technology to improve visibility and coordination between departments. Overall, this research confirms that Supply Chain Management is a key factor in improving the efficiency and operations of PT Benih Citra Asia. Cross-departmental collaboration and effective communication are critical elements in successful implementation of Supply Chain Management.

Biomass Accumulation, Soil Fertility and Energy Use in Seed Crop Systems of Velvet Bean (Mucuna Pruriens L.)

ABSTRACT Seed crop production systems of velvet bean (Mucuna pruriens L.) were assessed in two field studies for ecosystem services. At flowering stage, long duration genotype Arka Shubra was significantly superior in biomass accumulation (14.61 t ha−1), while 100% recommended NPK + 15 t FYM ha−1 registered significantly higher biomass production (11.74 t ha−1). Biomass availability at harvest was higher in long duration genotypes (7.48–7.75 t ha−1) and the average biomass availability of 6.45 t ha−1 at harvest gives scope for including velvet bean as component crop in cropping systems/fallows. The average soil carbon stocks were estimated at 22.8 t ha−1 at the end of 2 yrs and 12.4 t ha−1 at the end of one crop season. Soil nutrient availability was optimum except for soil test P in pre-, standing and post crop growth periods. Highly significant relations (R 2 = 0.949) were observed between nodule number and available N at harvest. The N availability was more in long duration genotypes (209–215 kg N ha−1) over pre-experimental status. Nutrient addition improved the available N status by 40–185 kg ha−1 over pre-experimental status and control. The nutrient uptake was 4–10% higher in Arka Shubra over other long duration genotypes and 100–200% higher over medium/short duration genotypes at flowering. The recyclable biomass and uptake of NPK are more in long duration genotypes implying huge nutrient recycling potential of the system. Overall, seed crop systems of velvet bean are efficient in resource use and energy use (>2.5 EUE) and ideal components for sustainable land management.

Enhancing Seed Access and Climate Resilience: A Farmer-Based Seed Multiplication Scheme in a Legume-Sorghum Double Cropping System in Mieso District Ethiopia

Sorghum is a major food security crop in Ethiopia. Sorghum landraces are particularly crucial in crop-livestock mixed farming systems in drylands, but due to the longer growing periods (6-8 months) needed for the landraces, the crop faces recurrent drought events associated with delayed rain, dry spells, and drought during critical stages. The landraces are normally grown combining a short and erratic rainy season (March to May) and a main rainy season (July to September). To address such circumstances, early-maturing improved sorghum varieties that are planted in July and mature within 3-4 months have been developed. Moreover, a sustainable cropping system involving legume-early maturing sorghum double cropping has been developed as an alternative to the traditional long-cycle sorghum production to help mitigate crop failure. The legume fits into the short rainy months. However, access to seeds of both component crops could be a challenge. The objective of this study therefore was to establish and assess the feasibility of a farmer-based seed multiplication (FBSM) scheme in enhancing the supply of quality seeds of legumes and sorghum and contributing to climate adaptation. Two activities were implemented in 2015 and 2016 in Mieso district, West Hararghe, Ethiopia. The first activity involved multiplying seeds of two early-maturing and drought-tolerant improved sorghum varieties (Dekeba and Melkam) using a FBSM scheme. The second activity was integrating this scheme into a legume-early maturing sorghum double cropping system. The results of the study show that the FBSM scheme provides much-needed support to smallholder sorghum farmers by offering resources for multiplying and marketing improved sorghum seeds, ensuring seed security, and improving access to quality seeds. The scheme aligns well with the legume-early maturing sorghum double cropping system, thereby enhancing access to compatible seeds and promoting a sustainable cropping system in the region. The scheme presents a viable solution to seed access for smallholder farmers in Ethiopia to enhance seed supply, income, and climate resilience, offering valuable insights for policymakers and stakeholders

Leveraging the potential of charred archaeological seeds for reconstructing the history of date palm

The analysis of seeds from archaeobotanical assemblages is essential for understanding the history of crop cultivation. However, the majority of these seeds are typically found charred, a condition that not only degrades DNA, which hinders genetic studies, but also distorts their morphological features, which may bias comparisons with uncharred modern samples. While the effects of charring on several other crop seeds or fruits are well-documented, date palm seeds remain largely unexamined, limiting our ability to use charred seeds effectively to document the agrobiodiversity dynamic of this crop.In this study, we assessed the morphological changes induced by the charring of 1375 Phoenix seeds under varied conditions, including charring temperatures of 200–600 °C, exposure durations from 10 to 120 min, and oxidizing/reducing conditions. By comparing charred samples with a modern reference collection of 6991 seeds, we evaluated the extent to which charring affects the ability to discriminate between groups of interest, particularly between wild and domesticated specimens.Our study identified a significant shrinkage in Phoenix seeds, up to 25%, as a result of charring, with the extent of deformation influenced by the conditions of exposure. The shrinkage displayed an isometric pattern, keeping the proportions between seed dimensions consistent, thereby validating size ratios as a dependable metric for studying Phoenix agrobiodiversity even when dealing with charred material. Moreover, seed outlines stay predominantly unchanged, further endorsing their utility in morphometric studies. Using our results to examine 13 charred seeds from Shahi Tump and Miri Qalat, two Protohistoric settlements (5th-3rd millennia BCE) located in the Kech-Makran district of southwestern Pakistan, we deduced a predominance of wild resource utilization over the cultivation of date palms.This research sheds light on the impact of charring on seed morphology and underscores its potential in differentiating between species and groups. It moreover confirms the value of charred seeds as a crucial resource for unraveling the complex history of crop cultivation, offering a detailed framework for future studies in this domain.

Characteristics of Soil Selenium-cadmium Migration and Accumulation and Its Bioeffectiveness in Typical Geological High Background Area

The prevalence of selenium-cadmium （Se-Cd） symbiosis in soils of geologically high background areas directly affects the safe utilization of Se-rich land resources. To investigate the migration and accumulation characteristics and bio-effectiveness of Se-Cd in the soil-crop system in typical geological high background areas of Southwest China and to realize the safe use of natural Se-rich land resources in geological high background areas, we collected 84 samples of agricultural crops （maize） and their supporting root systems and analyzed the Se-Cd content and physicochemical properties. Se-Cd accumulation characteristics, influencing factors, and bio-effectiveness of the soil-crop system were evaluated using geostatistics, bioenrichment factors, and geographic detectors. The results showed that the Se-Cd content in the study area was significantly higher than the background value of the soil in the whole country and in Yunnan Province. Influenced by the geological background, secondary enrichment in the process of soil formation, and agricultural activities, the accumulation and enrichment characteristics of Se in the root soil varied from no enrichment to slightly enriched, and the occurrence form was dominated by the residue state. The accumulation index of soil Cd was mainly in the medium pollution level, and the occurrence form was mainly in the residual state and the combined state of iron and manganese. The Se-enrichment rate of crop seeds reached 98.8% （DB 50/T 524-2013 standard）, and the average value of bioconcentration factor was 5.8%. The exceeding rate of Cd content in crop seeds was only 1.19% （GB 2762-2022 standard）, and the average value of Cd bioconcentration factor was 2.11%, so the ecological risk of heavy metal Cd in crop seeds was relatively low. In the Se-Cd symbiosis area under geological background, the weak alkaline environment of the soil could effectively reduce the bioavailability of Cd in crop seeds, and the Se-rich soil could inhibit the uptake of Cd by the crops to a certain extent. Correlation analysis showed that the migration and accumulation of Se and Cd from soil to crop seeds in the soil-crop system were affected by the elemental accumulation pattern and the physical and chemical properties （pH） of the soil, and at the same time, there was a certain synergistic-antagonistic effect between Se and Cd in the soil-crop system. Correlation analysis showed that the migration and accumulation of Se and Cd from soil to crop seeds in the soil-crop system was influenced by the occurrence of elements, soil physicochemical properties （pH）, and other factors, and there was also a certain synergistic-antagonistic interaction between Se and Cd in the soil-crop system.

Complete Mechanization of Oil Seed Crop Cultivation to Augment the Potentiality of Yellow Revolution in India: A Review

This study explores how mechanization could support oil seed production in India in line with the self-sufficiency objectives of the yellow revolution. The text highlights the limitations, such as low yields and reliance on imports, and highlights how mechanization can increase productivity, profitability, and resource usage. Particular mechanized techniques for different stages such as field preparation, sowing, weed management, and harvesting are explored with an emphasis on groundnut, Castor, Sunflower, Rapeseed, Soybean and sesame cultivation. This review paper promotes broader adoption of mechanization as a crucial tactic to accomplish sustainable growth in oil seed production and reduce dependence on imports.

Melatonin Priming Promotes Crop Seed Germination and Seedling Establishment Under Flooding Stress by Mediating ABA, GA, and ROS Cascades.

Both seed germination and subsequent seedling establishment are key checkpoints during the life cycle of seed plants, yet flooding stress markedly inhibits both processes, leading to economic losses from agricultural production. Here, we report that melatonin (MT) seed priming treatment enhances the performance of seeds from several crops, including soybean, wheat, maize, and alfalfa, under flooding stress. Transcriptome analysis revealed that MT priming promotes seed germination and seedling establishment associated with changes in abscisic acid (ABA), gibberellin (GA), and reactive oxygen species (ROS) biosynthesis and signaling pathways. Real-time quantitative RT-PCR (qRT-PCR) analysis confirmed that MT priming increases the expression levels of GA biosynthesis genes, ABA catabolism genes, and ROS biosynthesis genes while decreasing the expression of positive ABA regulatory genes. Further, measurements of ABA and GA concentrations are consistent with these trends. Following MT priming, quantification of ROS metabolism-related enzyme activities and the concentrations of H2O2 and superoxide anions (O2 -) after MT priming were consistent with the results of transcriptome analysis and qRT-PCR. Finally, exogenous application of GA, fluridone (an ABA biosynthesis inhibitor), or H2O2 partially rescued the poor germination of non-primed seeds under flooding stress. Collectively, this study uncovers the application and molecular mechanisms underlying MT priming in modulating crop seed vigor under flooding stress.

Peanut Rhizosphere Achromobacter xylosoxidans Inhibits Aspergillus flavus Development and Aflatoxin Synthesis by Inducing Apoptosis through Targeting the Cell Membrane.

Contamination of crop seeds and feed with Aspergillus flavus and its associated aflatoxins presents a significant threat to human and animal health due to their hepatotoxic and carcinogenic properties. To address this challenge, researchers have screened for potential biological control agents in peanut soil and pods. This study identified a promising candidate, a strain of the nonpigmented bacterium, Achromobacter xylosoxidans ZJS2-1, isolated from the peanut rhizosphere in Zhejiang Province, China, exhibiting notable antifungal and antiaflatoxin activities. Further investigations demonstrated that ZJS2-1 active substances (ZAS) effectively inhibited growth at a MIC of 60 μL/mL and nearly suppressed AFB1 production by 99%. Metabolomic analysis revealed that ZAS significantly affected metabolites involved in cell wall and membrane biosynthesis, leading to compromised cellular integrity and induced apoptosis in A. flavus through the release of cytochrome c. Notably, ZAS targeted SrbA, a key transcription factor involved in ergosterol biosynthesis and cell membrane integrity, highlighting its crucial role in ZJS2-1's biocontrol mechanism. Moreover, infection of crop seeds and plant wilt caused by A. flavus can be efficiently alleviated by ZAS. Additionally, ZJS2-1 and ZAS demonstrated significant inhibitory effects on various Aspergillus species, with inhibition rates ranging from 80 to 99%. These findings highlight the potential of ZJS2-1 as a biocontrol agent against Aspergillus species, offering a promising solution to enhance food safety and protect human health.

CDR1, a DUF946 domain containing protein, positively regulates cadmium tolerance in Arabidopsis thaliana by maintaining the stability of OPT3 protein

Industrial and agricultural production processes lead to the accumulation of cadmium (Cd) in soil, resulting in crops absorb Cd from contaminated soil and then transfer it to human body through the food chain, posing a serious threat to human health. Thus, it is necessary to explore novel genes and mechanisms involved in regulating Cd tolerance and detoxification in plants. Here, we found that CDR1, a DUF946 domain containing protein, localizes to the plasma membrane and positively regulates Cd stress tolerance. The cdr1 mutants exhibited Cd sensitivity, accumulated excessive Cd in the seeds and roots, but decreased in leaves. However, CDR1-OE transgenic plants not only showed Cd tolerance but also significantly reduced Cd in seeds and roots. Additionally, both in vitro and in vivo assays demonstrated an interaction between CDR1 and OPT3. Cell free protein degradation and OPT3 protein level determination assays indicated that CDR1 could maintain the stability of OPT3 protein. Moreover, genetic phenotype analysis and Cd content determination showed that CDR1 regulates Cd stress tolerance and affect the distribution of Cd in plants by maintaining the stability of OPT3 protein. Our discoveries provide a key candidate gene for directional breeding to reduce Cd accumulation in edible seeds of crops.

Effects of priming duration and rhizosphere bacteria metabolite concentration on the germinability of cowpea, soybean, sesame, and okra seeds.

Seed priming enhances germination and growth, which are important determinants of crop yield. This study was carried out to assess the effect of priming duration and metabolite concentration on the priming of five (5) different crops, using the metabolites of five (5) bacterial isolates. The crop seeds were treated in the cold-extracted metabolites of the five isolates at five (5) different priming durations (1, 2, 3, 4, and 5 h) and then in five metabolite concentrations (200, 400, 600, 800, and 1000 mg/L) of the five extracted metabolites at the optimal priming duration determined in the first experiment. Characterization of the cold-extracted metabolites was also carried out using gas-chromatography-mass spectrometry (GC-MS). Results revealed that priming cowpea and soybean for longer durations (< 3 h) could hinder their growth and development. Lower concentrations were observed to be optimal for cowpea and soybean, but for sesame and okra, there was no detectable pattern with metabolite concentration. The GC-MS revealed the presence of some molecules (e.g. hexadecanoic acid) that have shown plant growth promotion potential in other studies. This study showed that seeds with large endosperm, such as, cowpea and soybean, are more prone to the deleterious effects of treatment for longer durations. Further experiments should be carried out to isolate and purify the bioactive moieties for further studies and onward application.

Cover Crop Species Selection, Seeding Rate, and Termination Timing Impacts on Semi-Arid Cotton Production

By improving soil properties, cover crops can reduce wind erosion and sand damage to emerging cotton (Gossypium hirsutum L.) plants. However, on the Texas High Plains, questions regarding cover crop water use and management factors that affect cotton lint yield are common and limit conservation adoption by regional producers. Studies were conducted near Lamesa, TX, USA, in 2017–2020 to evaluate cover crop species selection, seeding rate, and termination timing on cover crop biomass production and cotton yield in conventional and no-tillage systems. The no-till systems included two cover crop species, rye (Secale cereale L.) and wheat (Triticum aestivum L.) and were compared to a conventional tillage system. The cover crops were planted at two seeding rates, 34 and 68 kg ha−1, and each plot was split into two termination timings: optimum, six to eight weeks prior to the planting of cotton, and late, which was two weeks after the optimum termination. Herbage mass was greater in the rye than the wheat cover crop in three of the four years tested, while the 68 kg ha−1 seeding rate was greater than the low seeding rate in only one of four years for both rye and wheat. The later termination timing produced more herbage mass than the optimum in all four years. Treatments did not affect cotton plant populations and had a variable effect on yield. In general, cover crop biomass production did not reduce lint production compared to the conventional system.

Prevalence and Diversity of Aphid-Vectored Yellow Dwarf Viruses in Oregon Perennial Grass Seed Crops

Oregon produces 70% of the world's cool-season grass seed, many species of which are susceptible to yellow dwarf viruses (YDVs). This virus complex has numerous defined virus species vectored by multiple aphid species. The diversity of YDVs associated with Oregon grass seed production has not been determined and is needed to provide insight to develop a risk prediction framework and improve management guidelines to mitigate disease severity. Commercial grass seed fields ( n = 57) across the Willamette Valley and central and eastern Oregon were surveyed across 2021 and 2022 to determine the incidence and diversity of YDVs associated with crop species (perennial ryegrass, tall fescue, and Kentucky bluegrass) and the abundance and incidence of YDVs associated with aphid vectors. Virus incidence and diversity were determined using multiple previously published endpoint reverse-transcriptase multiplexes specific for YDV genera and species. Across commercial grass seed crop hosts and growing seasons, 82% of fields had at least one plant sample detection of a luteovirus-type YDV, and 65% had at least one detection of a polerovirus-type YDV. Both perennial ryegrass and tall fescue crops hosted diverse YDV populations, which was especially apparent during the spring sampling season, when similarly diverse YDV communities were found associated with alate aphids. Only polerovirus-type YDVs were detected in Kentucky bluegrass, reflecting a variable landscape compared to Oregon's western Willamette Valley. This study provides a current understanding of the spatial composition and diversity of the aphid–YDV virus–vector system in grasses grown for seed in Oregon. [Formula: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 “No Rights Reserved” license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2024.