Poyang lake (PYL), as China's largest freshwater lake, has been suffering from increasing total phosphorus (TP) pollution associated with rapid basinal socio-economic development. However, anthropogenic phosphorus stressors were rarely examined in PYL basin due to its large-scale and complex river-lake connection water system, hampering phosphorus pollution control efforts. In this study, water pollution stress from multiple anthropogenic activities is quantitatively examined in PYL basin based on a newly developed framework coupling grey water footprint (GWF) analysis with the SPARROW model. Results show that the phosphorus source-sink process in PYL basin has been well simulated by SPARROW model quantifying an overall TP delivery rate of 0.39, with catchments closer to PYL showing higher delivery rate of phosphorus (up to over 0.8). The GWF analysis demonstrates that anthropogenic phosphorus sources have imposed much higher pollution stress on PYL than its inflowing rivers, with twelve catchments nearby PYL identified as critical source areas of TP contamination. Agricultural farming, livestock & poultry production, and urban household are recognized as the dominant anthropogenic stressors burdening water environment of PYL. Based on these, policy recommendations are provided for advancing control of the phosphorus pollution stressors. The methodology is effective in refined examination of water pollution sources, which is expected to be applied in other watersheds providing informative diagnosis of water issues especially in lakes.