Bacterial vaginosis (BV) is an ecological disorder of the vaginal microbiota that affects millions of women annually, and is associated with numerous adverse health outcomes including pre-term birth and the acquisition of sexually transmitted infections. This study aimed to compare between the different methods (Amsel's, Nugent's, Spiegel's and PAP smears) of BV diagnosis. The results revealed that from the 100 patients It was observed that 48 (48 %) were BV positive, they met three or four of Amsel's criteria . 56(56%) were diagnosed with BV and 45(45%) had normal vaginal flora based on Nugents' score . comparison between Nugent's score and Amsel's criteria for diagnosis of BV showed a sensitivity 81% and specificity 75% . when diagnosis bacterial vaginosis by Spiegel's criteria observed that 24 (24 %) of the 100 cases, were BV positive while 76 (76 %) cases were BV negative while 57 (57 %) cases were BV negative by using pap smear including presence of Bacterail vaginosis with Trichomonas & candida 16 (16%) , Bacterail vaginosis with Trichomonas 20(20%) and Bacterail vaginosis only 7 (7%) in BV positive cases. The comparison between Nugent's score and Pap smears for diagnosis of BV had a sensitivity of 62% and a specificity of 81% .