Crimson Seedless Research Articles

Influence of Different Rootstocks on Vine Growth, Cane Storage, and Yield in Crimson Seedless Grapevine

Influence of Different Rootstocks on Vine Growth, Cane Storage, and Yield in Crimson Seedless Grapevine

Enhancing Red Table Grape Coloration Using Tsikoudia: A Novel and Sustainable Approach.

Achieving optimal coloration in red table grapes, especially in warm-climate regions, presents significant challenges due to high temperatures that inhibit anthocyanin biosynthesis. Conventional methods to enhance grape coloration, including the use of abscisic acid (ABA), ethephon, foliar nutrient supplementation, and viticultural practices like cluster trimming and girdling, have limitations related to cost, regulatory restrictions, and potential adverse effects on grapes quality. This study proposes the application of tsikoudia, a traditional Greek alcoholic beverage, as a novel, sustainable, and cost-effective alternative to conventional practices. Tsikoudia, applied during the veraison stage, significantly improved the coloration of 'Crimson Seedless' and 'Red Globe' grapes by enhancing anthocyanin accumulation and altering color parameters. Specifically, lightness (L*), chroma (C*), and hue angle (h), measured using the CIE-Lab color system, were reduced, while the Color Index for Red Grapes (CIRG) was increased. Additionally, total anthocyanin content, determined through spectrophotometric analysis, also showed an increase. These changes indicate a more intense red coloration. This research highlights the effectiveness of tsikoudia in improving grape coloration and contributes to the development of more sustainable viticultural practices.

Leaf Retention Affects Photosynthetic Activity, Leaf Area Index, Yield and Quality of Crimson Seedless Grapes

Leaf Retention Affects Photosynthetic Activity, Leaf Area Index, Yield and Quality of Crimson Seedless Grapes

Development of an efficient molecular-marker assisted selection strategy for berry color in grapevine

Marker-assisted selection (MAS) represents one of the most effective strategies to reduce costs and time in plant breeding programs. MAS benefits from the identification of markers in strong linkage disequilibrium (LD) with causative genes and from the development of fast, scalable, and reproducible screening techniques. In grape, berry color is a valuable trait, highly related to the sensory characteristics of fresh grape and wine quality.The present work aims to develop a MAS strategy to select skin color by High-Resolution Melting (HRM), a ‘close-tube’ method capable of detecting polymorphisms within PCR-amplified DNA fragments. To identify molecular markers associated with the berry skin color phenotypic trait, the total genome of 3 white-berry and 3 red-berry accessions were sequenced using Illumina technology and aligned to the reference genome PN40024 v4. The sequencing results revealed the presence of three highly polymorphic regions on chromosome 2 between 15 Kbp and 17 Kbp for red berry genotypes. HRM analyses were carried out on a total of 95 genotypes, 70 of which comprised a berry color segregating population: [‘Italia’ (white berry) x cv ‘Crimson Seedless’ (red berry)] and 25 genotypes belonging to a commercial grapevine germplasm collection consisting of 11 red and 14 white berry accessions. The HRM profiles of the three regions tested strongly correlated with the phenotypic trait of color.The results of this work represent an effective, fast, and reliable genotyping strategy that enables the discrimination of genotypes based on the presence or absence of berry pigmentation.

Improving yield quality and coloration by using canopy management effect on microclimate within Crimson Seedless grapevines

Improving yield quality and coloration by using canopy management effect on microclimate within Crimson Seedless grapevines

Effect of Chitosan and Calcium Gluconate on Fruit Quality and Storability of Crimson Seedless Grape Cultivar.

Effect of Chitosan and Calcium Gluconate on Fruit Quality and Storability of Crimson Seedless Grape Cultivar.

Exogenous Melatonin Treatments Maintain Berry Quality and Bioactive Compounds of Minimally Processed Table Grapes (‘Crimson Seedless’) During Cold Storage

Exogenous Melatonin Treatments Maintain Berry Quality and Bioactive Compounds of Minimally Processed Table Grapes (‘Crimson Seedless’) During Cold Storage

Entomofauna presente en refugios vegetales en un campo de vid var. Crimson Seedless en Ica-Perú

The use of chemical pesticides to control pests also affects beneficial insects, so it is necessary to implement mechanisms that allow them to be protected and promote their development. In the present investigation, three plant shelters were installed to identify their entomofauna and determine the plant species with the potential to host biological controllers of grapevine pests. The study was carried out in the 2020-21 growing season, with seven plant species to make up three plant shelters (A, B, and C) that were installed on the edges of a vineyard of the Crimson Seedless variety in Ica, Peru. In total, 1,209 insects were reported, in six orders, nine families, and sixteen species; the most abundant were pollinators, followed by phytophagous, predators, and some parasitoids. There were no statistical differences between the shelters in terms of the abundance of parasitoids and pollinators, but there were in predators and phytophagous specifically between shelters A and B with C. Shelters A and B, which included the fennel Foeniculum vulgare Mill., stood out for having a larger population of predators, including Chrysoperla externa (Hagen), the main controller of the “vine mealybug” (Planococcus spp.). The importance of plant shelters was demonstrated and their implementation in vineyards is recommended to promote biological control and contribute to integrated pest management in this crop. Keywords: biodiversity, natural enemies, conservation biological control.

Improving Water Use Efficiency, Yield, and Fruit Quality of Crimson Seedless Grapevines under Drought Stress

Drought stress is a group of abiotic stresses that affects plant growth and yield production. A field experiment over two successive seasons (2021–2022 and 2022–2023) in sand soil was conducted to investigate the integration effect of deficit irrigation, soil amendment “hundzsoil”, and the spraying of proline on the water use efficiency (WUE), yield, and fruit quality of 8-year-old Crimson seedless table grapes. Four application rates of soil amendment (0, 2, 4, and 6 kg hundzsoil /vine) were added during the dormancy period, and four irrigation levels at 125, 100, 75, and 60% of the field capacity were applied just before flowering until harvest. Proline at two levels (0 and 500 ppm) was applied as a foliar spray. Parameters such as bud fertility, weight of 100 berries, juice volume, and cluster number were positively affected by irrigation at 75% FC along with applying hundzsoil at 2 and 4 kg/vine under proline spray in both seasons. Irrigation at the 125% FC level with a 6 kg hundzsoil application under proline spray resulted in the highest yield, berries number, cluster length, cluster weight, and total anthocyanin in both seasons. The TSS/acidity ratio was significantly and positively affected by deficit irrigation (60% FC level) under hundzsoil at a rate of 4 kg alongside proline spray. Reducing irrigation to 60% FC without hundzsoil and proline spray negatively affected numerous growth parameters and the yield. However, irrigation at 60% FC alongside 6 kg of hundzsoil and proline showed the highest IWUE in both seasons. Proline spray was a key factor in conserving water used for irrigation. This study recommends using deficit irrigation alongside hundzsoil application under proline spray as an adequate strategy for water use efficiency and improving the yield and fruit quality of Crimson seedless grapevines cultivated in sand soil.

Pre- and postharvest chitosan coatings extend the physicochemical and bioactive qualities of minimally processed 'Crimson Seedless' grapes during cold storage.

Food marketers desire residue-free fresh grapes although grapes have a short postharvest life. This study was performed to determine the influences of pre- and/or postharvest chitosan (Ch) coatings on postharvest quality of minimally processed (stem-detached) organic 'Crimson Seedless' berries. Berries were sorted as: (a) control (untreated berries); (b) preharvest Ch (dipping the clusters on the vine into 1% Ch 10 days before harvest at 20% soluble solid content (SSC)); (c) postharvest Ch (dipping the stem-detached berries into 1% Ch); and (d) pre + postharvest Ch. Berries were stored in 12 × 15 cm rigid polypropylene cups for up to 42 days at 1.0 ± 0.5 °C. Pre- and/or postharvest Ch coating reduced weight loss during storage. Pre- + postharvest Ch was the best treatment for restricting polygalacturonase (PG) activity, extending the visual quality, color features (L*, C and h°), skin rupture force, biochemical (SSC, titratable acidity, maturity index and pH) and bioactive (total phenol content, antioxidant activity) features. Pre- or postharvest Ch was also significantly effective in maintaining many quality features. Pre- and/or postharvest 1% Ch coatings effectively maintained the quality of minimally processed grape berries of organically produced 'Crimson Seedless' grapes by delaying weight loss and PG activity and keeping the postharvest physical, biochemical and bioactive features for 42-day cold storage at 1.0 ± 0.5 °C. The combined use of pre- and postharvest Ch found to be more effective than single treatment. Thus, pre- + postharvest 1% Ch coating could be recommended as an ecofriendly sustainable methodology for extending the postharvest quality of minimally processed fresh grapes. © 2024 The Author(s). Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Study on Quality of Verjuice Affected by Grape Types, Treatments and Storage at Ambient Conditions

To improve the quality of grape berries and bunches, berry thinning is adopted. In this practice, a sizeable quantity of thinned berries is destroyed despite having valuable contents. Thinned berries are utilized to produce verjuice in different countries. To assess the effect of grape varieties and ingredients on verjuice quality, the present study was conducted at ICAR-National Research Centre for Grapes, Pune during the fruiting season of 2022-2023. Thinned grape berries (6-8 mm diameter) from four varieties namely Manjari Naveen, Crimson Seedless, Thompson Seedless and Manjari Kishmish were utilized with three treatments as the addition of 0.49 g NaCl salt/100 mL, 3 mL olive oil/100 mL and combination of 0.49 g NaCl salt and 3 mL olive oil/100 mL. Physico-chemical parameters of prepared verjuice types were observed initially as well as 15, 30 and 45 days of storage at ambient conditions. At the initial stage, among the varieties, treatments and their interactions showed non-significant differences for TSS, acidity and pH. Among the different verjuice types, except Crimson Seedless with salt and olive oil, were recorded with maximum TSS. The pH values Were ranged from 2.35 to 2.57 while the maximum recorded titratable acidity was 3.07% whereas the lowest acidity was 2.43%. Verjuice storage at ambient conditions resulted in reduced TSS, acidity and pH. Verjuice prepared from Manjari Kishmish showed superiority in all sensory parameters while verjuice from Thompson Seedless was scored minimum. Results recorded in the present study revealed that the quality of verjuice was acceptable and need to study the combinations of different type grapes to improve verjuice quality and pesticide degradation in the process.

Varietal suitability of grapes in different agro-climatic regions of India

Grape cultivation, in India, is dependent on a narrow range of table grape varieties, with more than 70 per cent cultivated area in Maharashtra alone, followed by Karnataka and Tamil Nadu. Therefore, to increase the scope of area extension under grape cultivation, the suitability of six coloured grape varieties was assessed for yield, quality and phenological parameters at four locations, i.e., Pune (Maharashtra), Arabhavi (Karnataka), Rajendranagar (Telangana) and Theni (Tamil Nadu) during 2019-2022. From the study, it was revealed that the ‘Manjari Shyama’ and ‘Red Globe’ yielded the maximum (13.7 kg/vine) as compared to the rest of the varieties. Variety ‘Manjari Shyama’ was found stable at all four locations for yield, bunch weight, 100-berry weight, berry diameter, TSS, acidity, sugar: acid ratio and days to veraison with the regression coefficient 1.22, 1.11, 1.01, 1.13, 1.35, 1.44, 0.88 and 0.79, respectively. The yield and bunch weight in ‘Red Globe’ was found to be more expressive under the favourable environment as compared to ‘Fantasy Seedless’ (5.59 kg/vine), ‘Nanasaheb Purple Seedless’ (6.13 kg/vine) and ‘Crimson Seedless’ (7.63 kg/vine), which were found lower.

Influence of mixed and single infection of grapevine leafroll-associated viruses and viral load on berry quality.

Grapevine leafroll disease is a viral disease that affects grapevines (Vitis vinifera L.) and has a severe economic impact on viticulture. In this study, the effect of grapevine leafroll-associated viruses (GLRaV) on berry quality was investigated in clones of cultivar cv. Crimson Seedless table grapes infected with GLRaV. RT-PCR confirmed the identity of the clones: clone 3236, infected only with GLRaV-3 (termed single); clone 3215, infected with GLRaV-3, GLRaV-4 strain 9 and grapevine virus A (termed mixed); and a viral free clone of the same genetic background of the infected clones (termed control). The berry quality indices of size, sugar, acidity and anthocyanin content were measured at harvest maturity. RT-qPCR was used to determine the viral load. The study was repeated over 2year. A two-way, multivariate analysis of variance was applied with clone and year as independent variables and the measured berry quality parameters as a dependent variable. All dependent variables were significantly affected by viral infection (Wilks, λ, (2,33)=0.033895, P-value<0.001), while only titratable acidity was affected by year. The average berry dry mass decreased (P-value<0.001). The water content of both infected clones was greater than that of the control (P-value<0.001). Both infected clones displayed reduced sugar content as a fraction of the berry dry mass (P-value<0.001). The anthocyanin and the phenol content of the infected clones were significantly reduced compared with the control clone (P<0.001, P<0.05, clone 3236 and clone 3215, respectively). Finally, the viral load was highly variable, and no quantitative relationship between viral load and berry composition was found.

The Effects of Pre-Harvest Melatonin Applications on Phytochemical Properties of Crimson Seedless Grape Variety (V. vinifera L.)

Foliar melatonin applications are crucial for grape quality as they can enhance skin color development, increase antioxidant capacity and nutritional value of grapes. The effectiveness of preharvest melatonin applications may change depending on fruit species, variety, application time, and dose. In the current study, it was utilized from various doses of melatonin application, including 0, 0.25, 0.50, and 1 mmol l-1 for improving the phytochemical attributes of Crimson Seedless table grape variety. The results of the principal component analysis showed that different doses of foliar melatonin application had different effects on the yield and biochemical attributes of grape variety. But particularly, 1 mmol l-1 and 0.50 mmol l-1 of melatonin doses had significant effects on total phenolic compounds content and antioxidant capacity from the phytochemical properties of Crimson Seedless table grape variety.

Evaluation of Different Table Grape Varieties in Response to Growth and Physiological Traits during Foundation Bud Pruning

Grape (Vitis vinifera L.) was one of the first fruits cultivated by human since the dawn of civilization, the fermented product of grapes, wine has probably been an important way of consuming grapes. It is one of the commercially important sub-tropical fruit crop of peninsular India. It is the world’s most important fruit in terms of total production and economic stand point. The present research investigates the growth and physiological parameters like pruned biomass, cane length, cane diameter, leaf area, leaf area index, matured canes and chlorophyll content in different table grape varieties over a two year experimentation period (2021-22 and 2022-23) at Horticulture Research and Extension Centre, Tidagundi (Vijayapur), Karnataka. The experiment consisted of 10 treatments (V1 - Red Globe, V2 - Fantasy Seedless, V3 - Crimson Seedless, V 4 - Manjari Shyama (A-18/3), V 5 – Nana saheb Purple Seedless, V 6 - Sharad Seedless (Check), V 7 - Merbein Seedless, V 8 - 2A-Clone, V 9– Manjari Kishmish (Kishmish Rozavis White) V10 - Thompson Seedless (Check)) laid out in randomized block design with 4 replications. The results highlight the highest fresh weight (2.23 kg/vine), dry weight (1.38 kg/vine), maximum number of canes (53.31), mature canes (48.04), fruitful canes (43.52) per vine, highest leaf area (200.11 cm2) and leaf area index (3.91) were noted in Thompson Seedless. The maximum cane length (75.90 cm) was recorded in the variety Crimson Seedless. The maximum cane diameter (9.80 mm) was recorded in Red Globe. Manjari Kishmish recorded the maximum chlorophyll content of the leaf (42.92).

Calosidade do solo de mudas de uva enxertadas como uma característica positiva para as mudanças climáticas

Abstract Nowadays, some relative warming temperatures related to climate change may be provided at the grafting time. Therefore, this study was conducted during two seasons (2018-2019) to study the effect of three callusing method (callusing room, callusing soil, callusing tunnel) and four grafting date (15 Jan., 1Feb., 15 Feb., 1 Mar.) for early (Flame seedless), medium (Thompson seedless) and late (Crimson seedless) grape varieties on grafted grape cuttings as short methods for transplant production. The results indicated that, the early grapes variety achieved higher grafting success on 1st Feb. grafting date as well as the late grape variety in callusing room and callusing soil methods. Also, Callusing soil achieved grafted success by 72.9%, 68.55% and 77.94% compared to callusing tunnel 37.3%, 45.9% and 55% for Flame seedless, Thompson seedless and Crimson seedless, respectively as mean of both seasons. High grafting success resulted from the high content of indole and sugars, along with low phenol content before callusing stage, as well as high indole and low sugars of grafts partner after callusing stage. while, higher phenols was accumulated in rootstock after callusing stage. There is no antagonistic effect between grafts partners. Callusing soil may be considered as an eco-friendly, sustainable and cheaper alternative tool for callusing of grafts cuttings.

Rootstock impact on quality attributes and shelf life of crimson seedless grapes

Rootstock impact on quality attributes and shelf life of crimson seedless grapes

Viticultural Climate Indexes and Their Role in The Prediction of Anthocyanins and Other Flavonoids Content in Seedless Table Grapes

Background: Viticulture bioclimatic indexes like the Heliothermal Index (HI), Cool Night Index (CI), and Dryness Index (DI), can be used to assess the influence of climate on grapes’ quality. Methods: HI, CI, and DI + total seasonal irrigation were utilized to assess the effect of climate on the flavonoids content and composition of two Vitis vinifera seedless varieties, ‘Summer Royal’ and ‘Crimson Seedless’, both grown in Apulia (Southern Italy). Results: The flavonoids content was significantly affected by variety and climate conditions on the base of HI, CI, and DI + total seasonal irrigation. Factor analysis applied to climate indexes and flavonoids showed that anthocyanins and flavonols were negatively and positively correlated to CI in both varieties, respectively. Additionally, warmer night temperatures determined higher fla-van-3-ols. HI increase promoted anthocyanins, flavonols, and flavan-3-ols content in Crimson Seedless, whilst it induced an opposite trend in Summer Royal. Finally, DI + total seasonal irrigation showed to be positively linked to flavonols content and negatively linked to anthocyanins content just in the case of Crimson Seedless. Significant regression models were also determined between climate indexes and productive parameters (i.e., yield, TSS, TA, pH, bunch, and berry weight). Conclusions: Climate indexes HI, CI, and DI + total seasonal irrigation showed an effect on quality grape parameters like flavonoids and contributed to building predictive models when new climatic zones are going to be evaluated for the production of table grapes.

The Effects of Gibberellic Acid and Cane Girdling of Applications on Crimson Seedless Grape Variety of Cluster and Berry Characteristics

‘Crimson Seedless’ grape variety is a late maturity and small berry size. Berry size is one of the most important quality parameters of table grapes. This trial was carried out in the Crimson Seedless (Vitis vinifera L.) grower vineyard in Sarıgöl (dadağlı) locality of Manisa province in the 2022 vegetation period. A randomized complete block design was used with vines as blocks and clusters as the experimental unit’s four treatments and four replications. Vine bunches of Crimson Seedless grape variety, ten years old, were sprayed extensively with a GA3 (Gibberellic Acid) solution (20 mg L-1) on the 25th of April 10th of May, and 10th of June/2022, while girdling (G) was applied from removing 3 mm diameter bark all around from 10 cm below the top of the vine trunk at fruit set stage. Control (C) only water was sprayed. This study aimed to reveal gibberellic acid and cane girdling applications on Crimson Seedless cluster and berry characteristics. Harvest (oBrix 19) was done on the 10th of October/2022 and after that, the cluster weight (g), cluster length (cm), cluster width (cm), berry weight (g), berry length (mm), berry diameter (mm), berry length/berry diameter, total soluble solid, total acidity and maturity index were determined in fresh fruit samples randomly taken from each vine. It was found that all applications generally had statistically (P

Basal defoliation, salicylic acid and cyanocobalamin to ameliorate the physiological and biochemical characteristics of flood-irrigated ‘Crimson Seedless’ grapevines in a semi-arid Mediterranean climate

ABSTRACT There is a high retail demand for ‘Crimson Seedless’ grape. Cluster shape, berry size, colour, and sugar contents influence the overall fruit quality and marketability. In many commercial vineyards of flood-irrigated clay soils under warm and humid semi-arid climates, adequate irrigation may lead to an enhanced fruit set that could potentially be associated with restricted berry growth, compact clusters, and poor berry colour and taste. To assess the role of some agronomic practices that may influence the canopy microclimate, and affect primary and secondary metabolites, seven treatments arranged in a randomised complete block design (RCBD) system with three replicates each (three vines per replicate) were applied as follow; the control (T1), 5-leaf basal defoliation at pre-bloom (BDPB) (T2), BDPB + foliar spray (FS) of 200 mg · L–1 salicylic acid (SA) (T3), BDPB + FS of 20 mg · L–1 cyanocobalamin (CCA) (T4), 5-leaf basal defoliation at full bloom (BDFB) (T5), BDFP + SA (T6) and BDFB + CCA (T7). Foliar applications were applied at 1) 2 weeks after the beginning of vegetative growth, 2) full bloom and 3) veraison stages. The analysis of variance (ANOVA), the principle component analysis (PCA) and the two-way hierarchical cluster analysis (HCA) indicated that BDPB (T2) generally has a better effect than that of BDFB (T5). The most pronounced effect on vegetative growth (shoot length and leaf area), photosynthesis activity (leaf chlorophyll and carbohydrate contents), fruit weight and dimensions, and total yield was observed in the case of T3, followed by T6, whereas concerning berry firmness, colour (anthocyanins) and sensory characteristics (soluble solids: acids ratio, total sugars and phenols), the most pronounced effect was observed in the case of T4, followed by T7.