When it comes to start-ups-related researches in the past, it seems rare to find ones analyzing characteristics of manufacturing start-ups with the focus on a certain specific region. Then, the purpose of this study is to precisely investigate characteristics and current situation of manufacturing start-ups in Daegu-Gyeongbuk region, using regional level data. To do so, descriptive statistical analysis was conducted first by using micro firm-level data provided by Statistics Korea. And using DID model, the study analyzed job creation effects of manufacturing start-ups of time lag in the region. Then, long-term job creation effects were found in both region and its effects of Daegu region were more distinctive. As a result, implications for policies promoting the growth of manufacturing start-ups in the region are as follows. First, considering long-term job creation effects of manufacturing start-ups, policies should be focused on increasing survival rate of competitive start-ups at early stage other than fostering them in quantity. Second, joint governance of Daegu-Gyeongbuk region showing geographical proximity and similar characteristics of industrial structure is required to handle issues related to promotion of manufacturing start-ups. Third, types of new industry that Daegu-Gyeongbuk region wants to strategically foster are quite different from the fields where regional start-ups are most actively formed. Therefore, industrial base for creation of new industry needs be intensified by promoting manufacturing start-ups in the region. Lastly, regional competence as post BI(business incubator) should be strengthened, fully utilizing industrial agglomeration such as industrial complex within the region.