Creatine Kinase Response Research Articles

Electroencephalogram, creatine kinase, and lactate dehydrogenase responses during preslaughter handling in goats handled by trained and untrained handlers

Electroencephalogram, creatine kinase, and lactate dehydrogenase responses during preslaughter handling in goats handled by trained and untrained handlers

Almond Consumption Modestly Improves Pain Ratings, Muscle Force Production, and Biochemical Markers of Muscle Damage Following Downhill Running in Mildly Overweight, Middle-Aged Adults: A Randomized, Crossover Trial

BackgroundAlmonds promote cardiometabolic health benefits; however, the ergogenic effect of almond supplementation on exercise recovery is less explored. ObjectivesWe evaluated the impacts of raw, shelled, almonds on pain, muscle force production, and biochemical indices of muscle damage and inflammation during recovery from eccentrically biased exercise. MethodsUsing a randomized, crossover design, 26 healthy adults (37 ± 6 y) ran downhill (–10%) for 30 min at a heart rate corresponding to 65%–70% of maximal oxygen consumption followed by 3-d recovery periods after 8-wk adaptations to either ALMOND (2 oz/d) or isocaloric pretzel (CONTROL) feedings. Volunteers consumed the study food immediately following the run and each day during recovery. Fasted blood samples were collected, and pain and muscle function were tested before the downhill run and over 72 h of recovery. ResultsDownhill running elicited moderate muscle damage (Time: P < 0.001; η2 = 0.395) with creatine kinase (CK) peaking after 24 h (CONTROL: Δ + 180% from baseline compared with ALMOND: Δ + 171% from baseline). CK was reduced after 72 h in ALMOND (Δ – 50% from peak; P < 0.05) but not CONTROL (Δ – 33% from peak; P > 0.05). Maximal torque at 120°/s of flexion was greater (Trial: P = 0.004; η2 = 0.315) in ALMOND compared with CONTROL at 24 h (Δ + 12% between trials; P < 0.05) and 72 h (Δ + 9% between trials; P < 0.05) timepoints. Pain during maximal contraction was lower (Trial: P < 0.026; η2 = 0.225) in ALMOND compared with CONTROL after 24 h (Δ – 37% between trials; P < 0.05) and 48 h (Δ – 33% between trials; P < 0.05). No differences (P > 0.05) in vertical jump force, C-reactive protein concentrations, myoglobin concentrations, and total antioxidant capacity were observed between trials. ConclusionsThis study demonstrates that 2.0 oz/d of almonds modestly reduces pain, better maintains muscle strength, and reduces the CK response to eccentric-based exercise. This apparent effect of almond ingestion on exercise recovery has the potential to promote increased exercise adherence, which should be investigated in future studies.This trial was registered at the clinicaltrials.gov as NCT04787718.

Chronic almond nut snacking alleviates perceived muscle soreness following downhill running but does not improve indices of cardiometabolic health in mildly overweight, middle-aged, adults.

As a popular food snack rich in protein, fiber, unsaturated fatty acids, antioxidants and phytonutrients, almond nut consumption is widely associated with improvements in cardiometabolic health. However, limited data exists regarding the role of almond consumption in improving exercise recovery. Accordingly, we aimed to investigate the impact of chronic almond snacking on muscle damage and cardiometabolic health outcomes during acute eccentric exercise recovery in mildly overweight, middle-aged, adults. Using a randomized cross-over design, 25 mildly overweight (BMI: 25.8 ± 3.6 kg/m2), middle-aged (35.1 ± 4.7 y) males (n = 11) and females (n = 14) performed a 30-min downhill treadmill run after 8-weeks of consuming either 57 g/day of whole almonds (ALMOND) or an isocaloric amount (86 g/day) of unsalted pretzels (CONTROL). Muscle soreness (visual analogue scale), muscle function (vertical jump and maximal isokinetic torque) and blood markers of muscle damage (creatine kinase (CK) concentration) and inflammation (c-reactive protein concentration) were measured pre and post (24, 48, and 72 h) exercise. Blood biomarkers of cardiometabolic health (total cholesterol, triglycerides, HDL cholesterol, and LDL cholesterol), body composition and psycho-social assessments of mood (POMS-2 inventory), appetite and well-being were measured pre and post intervention. Downhill running successfully elicited muscle damage, as evidenced by a significant increase in plasma CK concentration, increased perception of muscle soreness, and impaired vertical jump performance (all p < 0.05) during acute recovery. No effect of trial order was observed for any outcome measurement. However, expressed as AUC over the cumulative 72 h recovery period, muscle soreness measured during a physical task (vertical jump) was reduced by ~24% in ALMOND vs. CONTROL (p < 0.05) and translated to an improved maintenance of vertical jump performance (p < 0.05). However, ALMOND did not ameliorate the CK response to exercise or isokinetic torque during leg extension and leg flexion (p > 0.05). No pre-post intervention changes in assessments of cardiometabolic health, body composition, mood state or appetite were observed in ALMOND or CONTROL (all p > 0.05). Chronic almond supplementation alleviates task-specific perceived feelings of muscle soreness during acute recovery from muscle damaging exercise, resulting in the better maintenance of muscle functional capacity. These data suggest that almonds represent a functional food snack to improve exercise tolerance in mildly overweight, middle-aged adults.

The Effect of Massage on the Exhausted Aerobic Exercise-Induced Muscle Damage Indicators in Healthy Young Men

Background: Muscle damage and soreness caused by intense exercise are problems experienced by sedentary people whose suitable solution is an essential topic in research. Objectives: This study aimed to evaluate the effect of massage on the response of recovery creatine kinase (CK) and lactate dehydrogenase (LDH) following exhausted aerobic exercise. Methods: A total of 20 sedentary university male students (26.9 ± 2.7 y, BMI: 23.1 ± 1.6) participated voluntarily in the study. The subjects were randomly divided into two groups of exercise with massage group (mas, n = 10) and a control group (con, n = 10). Subjects run on a treadmill to the point of exhaustion. After that, the group had effleurage and petrissage massage for 20 min. Then, serum CK and LDH were measured, and the results were extracted using analysis of variance with repeated measures and post hoc Bonferroni test at the P &lt; 0.05 level. Results: Both groups significantly increased CK and LDH levels after the exercise session. There was no significant difference between the two groups immediately after the massage intervention (P &gt; 0.05). In addition, there was a significant difference between the two groups 24 and 48 hours after massage in the control (P &gt; 0.05), while there was no significant change in the massage group (P &lt; 0.05). Conclusions: Based on the results, massage intervention prevented the increase of CK and LDH levels at 24 and 48 hours after the exhaustive exercise session, which indicates the beneficial effect of massage.

Fish oil supplementation fails to modulate indices of muscle damage and muscle repair during acute recovery from eccentric exercise in trained young males

ABSTRACT We aimed to investigate the influence of 4-wk of fish oil (FO) supplementation on markers of muscle damage, inflammation, muscle soreness, and muscle function during acute recovery from eccentric exercise in moderately trained males. Sixteen moderately-trained males ingested 5 g/d of FO (n = 8) or soybean oil (placebo) capsules (n = 8) for 4-wk prior to- and 3-d following an acute eccentric exercise bout. Eccentric exercise consisted of 12 sets of isokinetic knee extension and knee flexion. Indices of muscle damage, soreness, function and inflammation were measured at baseline and during exercise recovery. Eccentric exercise elicited an increase in muscle soreness (p < 0.010) and thigh volume (p < 0.001), and reduced peak isometric torque by 31.7 ± 6.9%, (p < 0.05, 95% CI 10.6–52.8) during 3-d of recovery. Blood omega-3 polyunsaturated fatty acid concentration was 14.9 ± 2.4% higher in FO than PLA (p < 0.01, 95% CI 9.8–20.1). However, FO did not ameliorate the cumulative creatine kinase response (expressed as AUC; p = 0.368), inflammation (p = 0.400), muscle soreness (p > 0.140), or muscle function (p > 0.249) following eccentric exercise. FO supplementation confers no clear benefit in terms of ameliorating the degree of muscle damage, or facilitating the muscle repair process, during acute eccentric exercise recovery. These data suggest that FO supplementation does not provide an effective nutritional strategy to promote exercise recovery, at least in moderately-trained young men. Abbreviations: ANOVA: Analysis of variance; AUC: Area under curve; CI: Confidence interval; CK: Creatine kinase; CMJ: Countermovement jump; COX: Cyclooxygenase; CRP: C-reactive protein; DHA: Docosahexaenoic acid; DOMS: Delayed-onset muscle soreness; EIMD: Exercise-induced muscle damage; En%: Energy percent; EPA: Eicosapentaenoic acid; FO: Fish oil; IL-6: Interleukin-6; LDH: Lactate dehydrogenase; LOX: Lipoxygenase; Mb: Myoglobin; mTOR: Mechanistic target of rapamycin; PLA: Placebo; ROM: Range of motion; ROS: Reactive oxygen species; SD: Standard deviation; SEM: Standard error of the mean; TNF-α: Tumour necrosis factor alpha; VAS: Visual analogue scale; Ω3-PUFA: Omega-3 polyunsaturated fatty acids; Ω6-PUFA: Omega-6 polyunsaturated fatty acids Highlights The anti-inflammatory properties of omega-3 polyunsaturated fatty acids, alongside their propensity to incorporate into the muscle phospholipid membrane underpins the idea that fish oil supplementation may attenuate muscle damage and promote muscle repair following eccentric-based exercise. Four weeks of high-dose (5 g/d) fish oil supplementation prior to eccentric exercise failed to attenuate the rise in creatine kinase concentration and muscle soreness during acute exercise recovery in physically-active young men. Future studies are warranted to investigate the efficacy of combining omega-3 polyunsaturated fatty acids with other nutrients (i.e. protein/amino acids) for the promotion of muscle recovery following eccentric-based damaging exercise.

Effects of daily static stretch training over 6 weeks on maximal strength, muscle thickness, contraction properties, and flexibility.

Static stretch training (SST) with long stretching durations seems to be sufficient to increase flexibility, maximum strength (MSt) and muscle thickness (MTh). However, changes in contraction properties and effects on muscle damage remain unclear. Consequently, the objective of the study was to investigate the effects of a 6-week self-performed SST on MSt, MTh, contractile properties, flexibility, and acute response of creatine kinase (CK) 3 days after SST. Forty-four participants were divided into a control (CG, n = 22) and an intervention group (IG, n = 22), who performed a daily SST for 5 min for the lower limb muscle group. While isometric MSt was measured in leg press, MTh was examined via sonography and flexibility by functional tests. Muscle stiffness and contraction time were measured by tensiomyography on the rectus femoris. Additionally, capillary blood samples were taken in the pretest and in the first 3 days after starting SST to measure CK. A significant increase was found for MSt (p < 0.001, η 2 = 0.195) and flexibility in all functional tests (p < 0.001, η 2 > 0.310). Scheffé post hoc test did not show significant differences between the rectus femoris muscle inter- and intragroup comparisons for MTh nor for muscle stiffness and contraction time (p > 0.05, η 2 < 0.100). Moreover, CK was not significantly different between IG and CG with p > 0.05, η 2 = 0.032. In conclusion, the increase in MSt cannot be exclusively explained by muscular hypertrophy or the increased CK-related repair mechanism after acute stretching. Rather, neuronal adaptations have to be considered. Furthermore, daily 5-min SST over 6 weeks does not seem sufficient to change muscle stiffness or contraction time. Increases in flexibility tests could be attributed to a stretch-induced change in the muscle-tendon complex.

Effects of treatment dosage of whole-body cryotherapy upon post-match recovery of endocrine and biochemical markers in elite rugby league players: An experimental study.

The use of whole-body cryotherapy (WBC) for athletic recovery is becoming increasingly popular despite the lack of evidence supporting the dosage parameters in its implementation. The aim of the current study was to investigate the dose-response effects of WBC following match-play in elite rugby league players. We observed endocrine (salivary cortisol and testosterone) and biochemical (creatine kinase) responses following three separate post-match recovery periods in elite rugby league players. Comparisons were made between a single exposure (3 min at -120°C to --135°C) of WBC to two consecutive exposures (2 × 3 min), to a control (no exposure) during the recovery trials. Recovery characteristics were measured 36 h prematch, immediately postmatch, and 60 h postmatch. Cortisol concentrations remained unchanged in its pattern of response during the postmatch recovery periods across all WBC doses. Testosterone concentrations increased significantly (p < 0.0005) at 60 h, in the WBC2 trial. The Testosterone:Cortisol ratio increased significantly (p < 0.0005) at 60 h in the WBC2 trial, while during the WBC0 trial it did not recover to baseline levels. No significant effect on creatine kinase concentration was observed, although a statistical trend was shown in WBC2 for improved reduction of this marker at 60 h. These findings suggest that two, consecutive exposures to WBC immediately following fatiguing rugby league competition appear to stimulate an increase to the anabolic endocrine profile of participants by 60 h post-match, and may reduce the CK concentration. Coaches and athletes should consider the treatment dosage of WBC when used to optimize the desired response following a high-stress environment.

Acute fatigue in endurance athletes: The association between countermovement jump variables and creatine kinase response

Acute fatigue in endurance athletes: The association between countermovement jump variables and creatine kinase response

Individual-Specific Relationship Between External Training and Match Load and Creatine-Kinase Response in Youth National Team Soccer Players.

Previous studies have examined the relationship between external load and creatine-kinase (CK) response at the team level. This study aimed to build individualized CK prediction models for elite youth national team soccer players. The CK response of youth soccer players can be categorized as being sensitive to micromovements (MM), high-velocity (HV) parameters, or the combination of both, measured during training sessions and matches. Prospective cohort study. Level 4. A total of 25 U16-U17 youth national team soccer players were monitored during training sessions and matches using global positioning system (GPS) units. Individual CK values were measured every morning from whole blood. The data set consisted of 57 ± 17 individual datapoints per player. Individual prediction models were used to examine the relationship between external load and consecutive CK changes. Numerous models were built for each player using MM, HV parameters, or the combination of both. The performance of the models was described by the R2 and the root mean square error (RMSE, U/l for CK values). The MM models were superior for 8 players (R2 = 0.68; RMSE = 113 U/l), followed by HV (8 players; R2 = 0.69; RMSE = 88 U/l) and the combined models (2 players; R2 = 0.64; RMSE = 141 U/l). For the remaining 7 players, the R2 of the models was <0.5. The recovery time between efforts was more important in the HV model. Players could be categorized on sensitivity to MM, HV movements, or the combination of both. These findings can be used to individualize postmatch recovery strategies and to optimize weekly training periodization to maximize match performance.

The repetition of semen collection does not affect the physiological and biochemical response to electroejaculation of anesthetized adult and yearling pampas deer (Ozotoceros bezoarticus) males

The aim of this study was to determine if multiple captures, general anesthesia and electroejaculation (EE) modify the heart rate (HR), pulse rate (PR), respiratory rate (RR), rectal temperature (RT), oxygen saturation, and the cortisol, alkaline phosphatase (AP) and creatine kinase (CK) responses to EE in adult and yearling pampas deer (Ozotoceros bezoarticus) males. Individuals were captured, anesthetized and electroejaculated on four occasions, once per season, from June to the following March (first to fourth collection respectively). Rectal temperature, as well as cortisol, AP and CK serum concentrations were determined before and after electroejaculation (BEE and AEE). The HR (P < 0 0.0001), PR (P < 0.0001), RR (P < 0.001), CK (P < 0.001) and cortisol concentration (P = 0.003) increased AEE, while RT (P = 0.004) decreased AEE. Heart rate increased in the first and third collections (P < 0.01), RR increased in the fourth collection (P < 0.001), and the highest values of RT were observed in the third and fourth collections (P < 0.0001). We concluded that repeated handling to collect semen did not have a sustained negative effect on the response to EE, which supports repeated semen collection from the same individuals.

The Food Nutrient Index and Inflammation Are Not Linked to Eccentric Exercise-Induced Muscle Damage

ObjectivesVariation in muscle damage following eccentric exercise is wide-ranging and largely unexplained. This study investigated linkages between eccentric exercise-induced increases in the muscle damage biomarker creatine kinase (CK) and diet quality, body composition and fitness test performance, diagnostic chemistries, oxidative stress, and inflammation.MethodsParticipants included 53 healthy, non-obese male (n = 30) and female adults (n = 23) (20–55 y, BMI < 30 kg/m2) who were not engaged in regular resistance training. After familiarization, participants engaged in a 90-min bout of whole-body eccentric exercise (90-EE) (17 exercises). Changes in muscle soreness (DOMS, 1–10 scale) and damage biomarkers (serum CK, myoglobin), exercise performance, oxidative stress (4-hydroxynonenal or 4HNE), inflammation (CRP, IL-18, IL-16, IL-10), serum cortisol, and diagnostic chemistries were monitored immediately post-90-EE, and then each morning during a 4-d recovery period. Blood samples were collected in an overnight fasted state, with serum CK, myoglobin, cortisol, and diagnostic chemistries measured at a clinical lab. IL-18, IL-6, IL-10, and 4HNE were assayed by ELISA. Participants entered food and beverage intake in 3-d food records with nutrient and flavonoid intake assessed and the Food Nutrient Index (FNI) calculated using eight under-consumed nutrients.ResultsThe 90-EE bout induced significant DOMS and muscle damage, inflammation and oxidative stress, and decreases in strength and anaerobic power. CK increased throughout the 4-d recovery period, with the highest levels measured on the 4th day (range, 60–17,040 U/L; mean ± SD, 1,565 ± 3,132 U/L). CK was correlated with other tissue damage biomarkers including aspartate aminotransferase (r = 0.946), lactate dehydrogenase (r = 0.884), and myoglobin (r = 0.763). Under linear regression analysis with non-tissue damage outcomes including FNI (68.7 ± 15.7), DOMS and serum cortisol emerged as the best predictors of post-90-EE CK variance [R2 = 0.367, F(2,50) = 14.48, p < 0.001].ConclusionsThe CK response to 90-EE varied widely between participants with 37% of the variance related to perceptions of muscle soreness and the stress hormone cortisol, but not diet quality, inflammation, oxidative stress, or fitness test performance.Funding SourcesMegaFood.

Creatine Kinase and Lactate Dehydrogenase Enzymes Response to Lactate Tolerance Exercise Test

PURPOSE: This study aimed to assess alterations in serum creatine kinase (CK) and lactate dehydrogenase (LDH) levels after performing a lactate tolerance exercise test (LTET) in elite male swimmers.METHODS: Fourteen male adolescent swimmers participated in this study. All subjects performed LTET (8×100-meter swimming) with a 1-minute recovery interval between eight trainings. Plasma CK and LDH (markers of muscle damage) levels were measured 30 minute before and 24 hours after the test. A paired t-test was used for statistical analysis of data.RESULTS: Plasma CK and LDH levels increased immediately after LTET as compared to the values 30 minutes prior to exercise (188.91±34.04 vs. 148.83±29.63 mg/dL, p=.029; 318.17±53.89 vs. 272.08±52.93 mg/dL, p=.010, respectively). Both CK and LDH levels displayed a decreasing trend 24 hours post-LTET; however, there was no significant difference immediately after the test.CONCLUSIONS: Plasma CK and LDH levels increased following LTET, which is representative of muscle damage.

Exercise-associated muscle cramps and creatine kinase responses after workload spikes in a professional soccer player: a case study

Purpose.Exercise-associated muscle cramps (EAMC) are characterized by intense pain and involuntary contractions of a single muscle or muscle group. While EAMCs may occur during and after exercise, their precise aetiology remains unknown. However, there are some potential risk factors, as the workload of physical training previously performed. The purpose of this case report was to evaluate the acute:chronic workload ratio (ACWR) and creatine kinase (CK) concentrations in a professional soccer player to verify the potential influence of recent training history on an extreme EAMC episode and subsequent muscle damage.Methods.A 21-year-old professional soccer player (body fat: 6.5%; body mass: 76 kg; height: 1.76 m) who experienced an extreme EAMC episode after the end of an official soccer match was monitored with session rating of perceived exertion before and after the EAMC episode and with post-match CK concentrations.Results.ACWR revealed several spikes on the days before the match, with the highest one observed on the match day. The CK concentrations recorded 35 and 53 hours after the EAMC episode were 262% and 182% higher, respectively, than the maximal CK concentrations recorded during the season (703 U/l).Conclusions.This case report illustrates, for the first time, how workload spikes, monitored with ACWR, preceded an extreme EAMC episode that was followed by an exacerbated muscle damage response. Some insights are provided in this case report for practitioners working in professional soccer to help them better manage similar cases.

The Impact of 120 Minutes of Soccer-Specific Exercise on Recovery

ABSTRACT Purpose: The extra-time (ET) period of soccer is competed during fixture congested schedules with often limited recovery time between matches. The aim of this study was to assess muscle damage recovery following 90- and 120-min (i.e., incorporation of ET) of simulated soccer match-play. Methods: Twelve semiprofessional soccer players completed 90 and 120-min treadmill-based soccer-specific exercise in a counterbalanced order. Creatine kinase (CK), creatinine, urea, aspartate aminotransferase, perceived muscle soreness, pain pressure threshold, reactive strength index, countermovement jump height, and isokinetic strength assessments of eccentric knee flexors at 60, 180 and 270 deg‧s−1 were taken at baseline and immediately-, 24, 48 and 72-hr post-exercise to assess recovery. Results: No significant between-trial interactions except for CK were found. Pairwise comparisons detected a 53% increase in CK at 24-hr (455 ± 29 μ∙L−1) following 120-min of simulated match-play vs. the corresponding post 90-min time-point (299 ± 29 μ∙L−1; p < .01). The 120-min trial caused a 58% higher CK response at 72-hr (244 ± 25 μ∙L−1) vs. post 90-min comparisons (154 ± 29 μ∙L−1; p = .02). No interaction effects were detected for any other recovery variables. Creatine kinase and perceived muscle soreness remained elevated up to 72-hr in both trials (p < .01). Conclusions: These data indicate that 120 min of simulated soccer match-play delays the time-course of CK recovery up to 72-hr post-match. However, 120 min of simulated soccer has no additional impact on functional recovery and perceived muscle soreness vs. 90 min. Recovery should be investigated following 90- and 120-min of actual match-play.Trial registration The study was pre-registered on the Open Science Framework (DOI: 10.17605/OSF.IO/VGU6T Date: 10/06/2019).

Individual-based Creatine Kinase Reference Values in Response to Soccer Match-play.

The aim of the present study was to determine the creatine kinase reference limits for professional soccer players based on their own normal post-match response. The creatine kinase concentration was analyzed in response to official matches in 25 players throughout a 3-year period. Samples were obtained between 36-43 hours following 70 professional soccer matches and corresponded to 19.1±12.1 [range: 6-49] samples per player. Absolute reference limits were calculated as 2.5th and 97.5th percentile of the samples collected. Creatine kinase values were also represented as a percentage change from the individual's season mean and represented by 90th, 95th and 97.5th percentiles. The absolute reference limits for creatine kinase concentration calculated as 97.5th and 2.5th percentiles were 1480 U.L-1 and 115.8 U.L-1, respectively. The percentage change from the individual's season mean was 97.45±35.92% and players were in the 90th, 95th and 97.5th percentiles when the percentages of these differences were 50.01, 66.7, and 71.34% higher than player's season mean response, respectively. The data allowed us to determine whether the creatine kinase response is typical or if it is indicative of a higher than normal creatine kinase elevation and could be used as a practical guide for detection of muscle overload, following professional soccer match-play.

Running Performance of High-Level Soccer Player Positions Induces Significant Muscle Damage and Fatigue Up to 24 h Postgame.

This study aimed to determine the impact of a soccer game on the creatine kinase (Ck) response and recovery and the specific Global Positioning System (GPS)-accelerometry-derived performance analysis during matches and comparing playing positions. A sample composed of 118 observations of 24 professional soccer teams of the Brazil League Serie A was recruited and classified according to playing positions, i.e., Left/Right Defenders (D = 30, age: 25.2 ± 5.8 years, height: 187 ± 5.5 cm, weight: 80 ± 5.8 kg), Offensive Midfielders (OM = 44, age: 25.1 ± 0.2 years, height: 177 ± 0.3 cm, weight: 73 ± 1.2 kg), Forwards (F = 9, age: 25.1 ± 0.2 years, height: 176.9 ± 4.3 cm, weight: 74.5 ± 2.1 kg), Left/Right Wingers (M = 23, age: 24.5 ± 0.5 years, height: 175 ± 1.1 cm, weight: 74 ± 4.4 kg), and Strikers (S = 12, age: 28 ± 0.2 years, height: 184 ± 1.0 cm, weight: 80 ± 1.4 kg). Blood Ck concentration was measured pre-, immediately post-, and 24 h postgame, and the GPS-accelerometry parameters were assessed during games. Findings demonstrated that Ck concentrations were higher at all postgame moments when compared with pregame, with incomplete recovery markers being identified up to 24 h after the game (range: 402–835 U/L). Moreover, Midfielders (108.6 ± 5.6 m/min) and Forwards (109.1 ± 8.3 m/min) had a higher relative distance vs. other positions (100.9 ± 10.1 m/min). Strikers [8.2 (8.1, 9.05) load/min] and Defenders [8.45 (8, 8.8) load/min] demonstrated lower load/min than Wingers [9.5 (9.2, 9.8) load/min], Midfielders [10.6 (9.9, 11.67) load/min], and Forwards [11 (10.65, 11, 15) load/min]. These results could be used to adopt specific training programs and recovery strategies after match according to the playing positions.

Salivary And Plasmatic Creatine Kinase And Lactate Dehydrogenase Responses Following High-intensity Continuous Exercise

Salivary And Plasmatic Creatine Kinase And Lactate Dehydrogenase Responses Following High-intensity Continuous Exercise

Effect of one-week supplementation of Citrulline-malate, L-arginine and their combination on CK, LDH and CRP levels in male wrestlers following simulated wrestling test

Background: The aim of this research was to evaluate the effect of one-week supplementation of citrulline-malate, L-arginine and their combination on aerobic and anaerobic powers, CK, LDH and CRP levels in male wrestlers. Methods: In this experimental study, 40 male wrestlers (aged 19-25 years; weight 60-90 kg; body fat percent 12-20%) in a randomized and double-blind design were divided in four equal citrulline-malate (Cit; 4g/days), L-arginine (Arg; 4g/days), their combination (2g/days Arg + 2g/days Cit) and placebo (dextrose; P) group. Before and after supplementation one week period, all subjects were participated in a competition like wrestling. Each test consisted of two 3 min periods of arm-cranking at maximal executable cadence against resistance of 0.1 kg ∙ kg−1 body mass interspersed with 30-s recovery periods between consecutive tests. Blood samples were obtained in the 4 phases: one hour before and 5 min after two competitions like wrestling protocols. Creatine kinase (CK), C-reactive protein (CRP) and lactate dehydrogenase (LDH) were analyzed. Change any of the parameters (mean and standard deviation) of four stages repeated ANOVA and post hoc tests. The significance level was set at p&lt;0.05. Results: Based on the present results, the Cit and Arg + Cit supplementation attenuated the exercise-induced increase in response of CK (p&lt;0.05). However, the Arg supplementation had not any significant effect on the exercise-induced increase response of serum CK. Also, the Arg, Cit and Arg + Cit supplementation had not any significant effect on the exercise-induced increase response of total serum LDH and CRP. Conclusion: Based on the present results, it can be concluded that the one week citrulline-malate with L-arginine supplementation is more effective than the citrulline-malate or L-arginine supplementation to overcome the exercise-induced adverse responses in some muscle injury parameters. Therefore, combination of citrulline-malate and L-arginine supplementation is recommended to reduce exercise-induced adverse consequences.

The time course of recovery of indirect markers of exercise-induced muscle damage induced by multi- and single-joint exercises

Strength training is performed using multi-joint (MJ) or single-joint (SJ) exercises; however, it is not clear whether different time of recovery is necessary between the two types of exercise. The aim of the present study was to compare the time course of recovery of indirect markers of exercise-induced muscle damage (EIMD) in the elbow flexors after performing MJ and SJ exercises. Twenty-four (n = 24) untrained men were randomized in to the MJ (n = 12) or SJ group (n = 12). Exercise protocol to induce muscle damage consisted of four sets of ten repetitions at 80% of one repetition maximum (1RM) in front pull-down (MJ exercise) or biceps curl (SJ exercise). Maximal voluntary isometric contraction, muscle soreness during elbow extension, ultrasound imaging (muscle thickness and echo intensity) and creatine kinase (CK) were measured before and up to 96 h after exercise. Significant effect of time (p < 0.05) at all times after exercise was observed for isometric strength, muscle soreness, muscle thickness and at 48, 72 and 96 h for echo intensity, with no time × group interaction. However, significant time × group interaction (p = 0.03) was observed only for CK activity at 96 h (MJ = 3348 ± 2911 IU/l vs. SJ = 890 ± 1426 IU/l; p < 0.05). In addition, there was a significant increase in CK after MJ at 48 h, 72 h and 96 h after exercise (p < 0.05), while SJ increased only at 48 h after exercise. Despite a dissimilar time course of CK response, MJ and SJ exercises induced a similar recovery pattern for muscle strength, thickness, echo intensity and soreness.

Factors Influencing Creatine Kinase Response in Youth National Team Soccer Players

Previous studies have examined the relationship between external training load and creatine kinase (CK) response after soccer matches in adults. This study aimed to build training- and match-specific CK prediction models for elite youth national team soccer players. Training and match load will have different effects on the CK response of elite youth soccer players, and there will be position-specific differences in the most influential external and internal load parameters on the CK response. Prospective cohort study. Level 4. Forty-one U16-U17 youth national team soccer players were measured over an 18-month period. Training and match load were monitored with global positioning system devices. Individual CK values were measured from whole blood every morning in training camps. The dataset consisted of 1563 data points. Clustered prediction models were used to examine the relationship between external/internal load and consecutive CK changes. Clusters were built based on the playing position and activity type. The performance of the linear regression models was described by the R2 and the root-mean-square error (RMSE, U/L for CK values). The prediction models fitted similarly during games and training sessions (R2 = 0.38-0.88 vs 0.6-0.77), but there were large differences based on playing positions. In contrast, the accuracy of the models was better during training sessions (RMSE = 81-135 vs 79-209 U/L). Position-specific differences were also found in the external and internal load parameters, which best explained the CK changes. The relationship between external/internal load parameters and CK changes are position specific and might depend on the type of session (training or match). Morning CK values also contributed to the next day's CK values. The relationship between position-specific external/internal load and CK changes can be used to individualize postmatch recovery strategies and weekly training periodization with a view to optimize match performance.