Fruit Cracking Research Articles

Genome-wide association study of pineapple breeding population

Pineapples (Ananas comosus (L.) Merr.) are one of the most important tropical fruits. Although pineapple breeding program has been conducted in Japan, sufficient genomic information has not been adopted for breeding. The objective of this study was to identify quantitative trait loci (QTL) for pineapple breeding-related traits that can be applied to different populations. Here, we performed a genome-wide association study using seven breeding populations, including 703 individuals with genome-wide 9574 SNPs. Eleven QTLs were detected for nine traits (flesh color, flesh color values (a*, b*), bolting days, days from bolting to harvest, fruit shell color, soluble solid content, acidity, ascorbic acid content, and fruit cracking). Phenotypic value of each genotype in each population was confirmed for each QTL. Six QTLs (acidity QTL ACID-1, ascorbic acid QTL AsA-1 and AsA-2, flesh color QTL FCOL-1 and FCOLb-1, and fruit-cracking QTL CRACK-1) showed significant intergenotypic variance among all populations with genotype segregation. The obtained QTLs and their genotype information will contribute to the selection of cross-breeding parents and marker-assisted selection in pineapple breeding programs.

CaGAUT1, a main QTL gene, positively regulates fruit cracking in pepper

CaGAUT1, a main QTL gene, positively regulates fruit cracking in pepper

Comparative Effects of Calcium, Boron, and Zinc Inhibiting Physiological Disorders, Improving Yield and Quality of Solanum lycopersicum.

Localized calcium deficiency at the tomato flower end causes a physiological disorder called blossom end rot, resulting in yield losses of up to 50 percent. Fruit cracking is another physiological disorder of tomatoes that most often occurs when the movement of water and solutes to the tomato is protracted or rapid, but the underlying cause of fruit cracking is, again, calcium deficiency. Therefore, the present field experiment was conducted with the aim of increasing yield and reducing physiological disorders in tomatoes with a foliar application of calcium and micronutrients (zinc and boron). Four levels of calcium (0, 0.3, 0.6, and 0.9%), three levels of boron (0, 0.25, and 0.5%), and three levels of Zinc (0, 0.25, and 0.5%) were applied foliarly three times (starting at flowering, the 2nd application was repeated when the fruits set, and the 3rd after a period of 15 days from the fruits set). An addition of 0.6% calcium increased yield and associated traits with a decreased flower drop. Likewise, a 0.9% calcium addition increased fruit Ca content and decreased blossom end rot, fruit cracking, and Zn content. Foliar spraying with 0.25% boron (compound B) improved flowering and production while reducing flower drop and tomato fruit cracking. Similarly, an application of 0.5% B significantly increased Ca and B content with minimal blossom end rot and Zn content. Likewise, a 0.5% Zn application resulted in yield and yield-related traits with increased fruit B and Zn contents while blossom end rot, fruit cracking, and fruit Ca content were lower when 0.5% of foliar Zn was applied. Therefore, it is concluded that a foliar application of Ca, B, and Zn can be used alone or in combination to minimize the physiological disorders, increase production, and improve tomato fruit quality.

Enhancing sweet cherry resilience to spring frost and rain-induced cracking with pre-harvest melatonin treatments

Sweet cherry producers annually confront climatic challenges such as spring frost and fruit cracking. This vulnerability arises primarily from spring frost during bloom or cracking at critical maturity stages during persistent rainfall. With changing climate patterns, innovative strategies are essential to mitigate these adversities. Foliar applications of melatonin (MT) at 0, 0.01, 0.05 and 0.1 mM were tested on the ‘Prime Giant’ and ‘Sweetheart’ cultivars over four different production cycles (2020–2023) to evaluate the effect on frost resilience on flower buds and fruit cracking reduction. MT-treated flower buds showed reduced malondialdehyde content and increased fruit set in most seasons, reducing their vulnerability to extreme weather events. In addition, MT consistently decreased sweet cherry cracking incidence across all studied seasons, indicating a strong effect between the fruit ripening stage and susceptibility to fruit cracking, which was cultivar dependent. Quality parameters at harvest, including fruit firmness, and colour at harvest, were either delayed or unaffected in MT-treated fruits compared with controls. However, other ripening parameters were stimulated by pre-harvest MT applications in several growing cycles, such as total soluble solids, which slightly reduced total acidity in MT-treated fruits. In summary, pre-harvest MT treatments can be a promising strategy for climate change adaptation and stress mitigation, potentially increasing sweet cherry production under extreme weather conditions.

Enhancing the fruit yield and quality of pomegranate in a new niche area: Insights into site specific agronomic practices

The sustained higher profitability of a pomegranate orchard relies heavily on the production of a greater proportion of high-quality fruits, a goal achievable through the implementation of effective management practices. The objective of this study is to provide site-specific supplementary knowledge regarding the response of pomegranate plants to various management practices under arid conditions. With the aim of enhancing both marketable fruit yield and quality of pomegranate in arid regions, four separate and simultaneous experiments were conducted in the same orchard to evaluate (i) the response of planting materials derived from tissue culture, air layering, and cutting; (ii) the impact of crop (fruit) load; (iii) the response of crop regulation; and (iv) the application response of organo-mineral fertilizers containing potassium (OMF-K) and phosphorus (OMF-P). The results obtained for plant growth, fruit yield, and fruit quality did not exhibit significant differences among pomegranate plants raised through the three different methods of vegetative propagation. Striking results in terms of producing superior-grade fruits, higher marketable yield, and overall fruit quality were achieved with crop loads of 80 fruits per plant. Staggering the crop regulation from June to September effectively extended the harvesting season to four months, from the last week of November to March, with eventual benefit of reduced fruit cracking compared to the standard two-month harvesting period obtained from normal regulation. Soil application of the indigenously developed OMF-K in two equal splits, along with the recommended N and P through inorganic sources, significantly reduced fruit cracking (only 6.23%) compared to the recommended NPK through inorganic sources (26.9%), while maintaining similar physicochemical quality attributes.

Correlation between Sweet Cherry Quality Attributes and Fruit Cracking Index

Sweet cherry skin cracking, particularly due to rainfall, presents a significant challenge for commercial production. This issue impacts fruit quality and marketability, making it crucial to identify effective, accessible solutions tailored to local climatic conditions. Understanding the relationship between fruit quality parameters and cracking susceptibility is essential for supporting farmers and improving production practices. However, in an attempt to obtain higher quality fruit, it is possible that sometimes, especially if there is significant rainfall during the fruit ripening period, producers neglect the possibility that the fruit is much more susceptible to cracking. A two-year experience (2022-2023) investigated how certain quality parameters—such as fruit weight, firmness, pH, total soluble solids (TSS), fruit maturity index, and color —affect the cracking of sweet cherries. The research focused on three cherry cultivars (‘Ferrovia’, ‘Kordia’, and ‘Skeena’) grafted onto the ‘IP-C8’ rootstock. By examining these parameters, the study sought to identify factors that contribute to cracking and offer insights for improving fruit quality. The ‘Kordia’ cv. had the highest induced cracking index (1.67), followed by the ‘Skeena’ cv. at 1.27, and ‘Ferrovia’ cv. with a lower value of 0.27. Higher values indicate that ‘Kordia’ and ‘Skeena’ are more susceptible to cracking under stress conditions compared to ‘Ferrovia’. The induced cracking index highlights how cultivars respond to stress conditions, such as heavy rainfall. The ‘Kordia’’s higher value suggests it is more vulnerable to cracking under stress compared to ‘Ferrovia’ cv., which might be more resilient.

Assessment of Hast Bahar (Flowering Season) for Yield and Quality of Pomegranate at Farmer’s Field

An on-farm trail (OFT) on assessment of flowering season of pomegranate was tested. The experiment was conducted with three treatments comprising mrig bahar flowering as a control and hast bahar flowering with and without application of micronutrients and kaolin clay during June to February for three consecutive years from 2019–2022 in the different farmer fields at the Tharad taluka of Banskantha district, Gujarat, India. The treatment T1 Mrig bahar (June-July) flowering recorded the maximum total number of flowers (293.67) hermaphrodite flowers (108.9) and fruit setting (71.85%). Further, T1 exhibited the highest average number of fruits plant-1 (79.0) and maximum fruit yield (11.78 kg plant-1) followed by T3 and T2 treatments (10.14 kg plant-1 and 9.02 kg plant-1) respectively. In terms of total fruit yield, Treatment T1 exhibited the highest fruit yield of (9813 kg ha-1). Whereas, maximum individual fruit weight 179.44 g plant-1 and Total Soluble Solids (15.75°Brix) were found in Treatment T3. Moreover, treatment T3 Hast Bahar (Sept–Oct) flowering with foliar spray of micromix 0.2% and kaolin Clay 4.0%) has the lowest average fruit cracking (6.30%) and the lowest incidence of sunburn (8.80%) across the three years. However, treatment T1 recorded highest average fruit cracking (18.90%). Further, during the Mrig bahar season, incidence of leaf spots and fruit rot was observed higher compared to the Hast bahar flowering. In case of economics, treatment T3 obtained the highest average income of 411813 ha-1 as compared to treatment T2 and T3 ` 353074 ha-1 and 333425 ha-1, respectively.

Ecofriendly treatments to improve pomegranate trees production and tolerance to climate change

BackgroundPomegranate is capable of growing in different agro-climatic conditions ranging from tropical to sub-tropical. Where vegetative growth, nutritional and physiological status of pomegranate plants oscillate at high temperatures waves in the summer season. While we can overcome the cracking and sunburn of fruits, which are the main problems confronting pomegranate cultivars due to their significant impact on the quality of fruits.MethodsThe present research was conducted over the course of two consecutive seasons in 2021 and 2022. The study focused on ten-year-old trees that were cultivated in sandy soil inside a private orchard situated 70 km away from Cairo on the Cairo-Alexandria desert road in Egypt (30o13′31.4″N30o39′09″E). The aim of the present work was to evaluate the effect of foliar spraying of Wonderful pomegranate trees with some nutrients (B, Zn, Fe, Mn, Ca and Cu), amino acids and irradiated licorice on vegetative growth, fruit physical, quality and chemical and leaf chemical parameters. A randomized full-block design with three duplicates was employed in the study. Using the recently computed least significant difference values at the 5% level, averages were compared.ResultsThe results indicated that foliar spraying with nutrients, amino acids and licorice extract (irradiated or not) alone or in combination significantly increased the vegetative growth and leaf total chlorophyll content compared to the control. Also, they significantly increased fruit length, diameter, weight, and number/tree and thus yield. As compared to the control, all treatments significantly reduced the number of sunburned and cracked fruits leading to decrease the percentage of un-marketable fruits. The juice content of anthocyanin, total sugars and total soluble solids increased significantly in response to all treatments compared to the control while the acidity% greatly reduced. When these treatments were used instead of the control, the content of N, P, K, Fe, Zn, and Mn in the leaves was also positively increased.ConclusionsIn conclusion, foliar spraying of “Wonderful” pomegranate trees with a mixture of nutrients (B, Zn, Fe, Mn, Ca, Cu) and amino acids or irradiated licorice extract can be considered as a suitable management of the problem of sunburned or cracked fruits while improving the physical and chemical fruit quality with increased productivity.

Effect of NAA and ZnSO4 on Cracking and Chemical Properties of Litchi Fruits

Effect of NAA and ZnSO4 on Cracking and Chemical Properties of Litchi Fruits

Mitigating citrus fruit cracking: the efficacy of chelated calcium or silicon foliar fertilizers in 'Okitsu no. 58' citrus fruit.

The 'Okitsu No. 58' citrus variety is highly prone to fruit cracking, which jeopardizes yield and results in economic losses. In this study, we investigated the impacts of spraying 5 distinct concentrations (0.1, 0.2, 0.3, 0.4, and 0.5 g/L) of chelated calcium (Ca) or silicon (Si) fertilizers at the young fruit stage (60-90 days after flowering, DAF) on fruit cracking and quality in the citrus variety 'Okitsu No. 58'. The results showed either Ca or Si fertilizer treatments reduced fruit cracking. We found that all Ca and partial Si treatments (0.4 and 0.5 g/L) significantly promoted the accumulation of Ca content in the peel. Notably, Ca or Si treatments significantly reduced polygalacturonase (PG) activity and inhibited the production of water-soluble pectin (WSP) in the peel. Additionally, Ca or Si treatments elevated the superoxide dismutase (SOD) activity and decreased the malondialdehyde (MDA) content of the peels. Changes in these parameters likely contributed to strengthening the durability of peel cell wall constituents, thus enhancing the fruit's resistance to fruit cracking. Overall, except for the C3 (0.3 g/L of Ca), Ca or Si fertilizers contributed to fruit conventional quality, mainly in terms of higher soluble sugars (SS) and SS/TA (titratable acid). Therefore, our findings will provide a reference for the prevention and control of citrus fruit cracking and the development of new fertilizers.

Occurrence of Wetness on the Fruit Surface Modeled Using Spatio-Temporal Temperature Data from Sweet Cherry Tree Canopies

Typically, fruit cracking in sweet cherry is associated with the occurrence of free water at the fruit surface level due to direct (rain and fog) and indirect (cold exposure and dew) mechanisms. Recent advances in close range remote sensing have enabled the monitoring of the temperature distribution with high spatial resolution based on light detection and ranging (LiDAR) and thermal imaging. The fusion of LiDAR-derived geometric 3D point clouds and merged thermal data provides spatially resolved temperature data at the fruit level as LiDAR 4D point clouds. This paper aimed to investigate the thermal behavior of sweet cherry canopies using this new method with emphasis on the surface temperature of fruit around the dew point. Sweet cherry trees were stored in a cold chamber (6 °C) and subsequently scanned at different time intervals at room temperature. A total of 62 sweet cherry LiDAR 4D point clouds were identified. The estimated temperature distribution was validated by means of manual reference readings (n = 40), where average R2 values of 0.70 and 0.94 were found for ideal and real scenarios, respectively. The canopy density was estimated using the ratio of the number of LiDAR points of fruit related to the canopy. The occurrence of wetness on the surface of sweet cherry was visually assessed and compared to an estimated dew point (Ydew) index. At mean Ydew of 1.17, no wetness was observed on the fruit surface. The canopy density ratio had a marginal impact on the thermal kinetics and the occurrence of wetness on the surface of sweet cherry in the slender spindle tree architecture. The modelling of fruit surface wetness based on estimated fruit temperature distribution can support ecophysiological studies on tree architectures considering resilience against climate change and in studies on physiological disorders of fruit.

Investigation on the environmental causes of tomato fruit cracking and its propagation prediction in greenhouse.

In this study, Provence tomato variety was chosen for investigating the environmental causes of tomato fruit cracking, cracks characteristics, and their propagation prediction in a greenhouse. Fruit bagging approach was used to alter the temperature and humidity and to create a microclimate around the fruit to induce fruit cracking for testing. Results showed that the fruit cracking rate increased when the environment temperature exceeded 30°C, and the difference between the highest and lowest temperature values in a day was greater than 20°C. The cracking rate was aggravated when the difference between the highest and lowest humidity values in a day was less than 20%. The proportions of top cracking, longitudinal cracking, ring cracking, radial cracking, and combined cracking were 5.4%, 16.1%, 28.3%, 26.8%, and 32.1%, respectively. The fruit shoulder was the most susceptible region to crack, followed by fruit belly and top regions, whereas longer cracks were observed in the fruit belly region indicating a higher propensity to crack propagation in that region. Finally, the measured data were used to validate an extended finite element method developed to effectively predict cracking susceptibility and propagation in tomato fruit with a relative error of 4.68%.

Physicochemical Characterization and Nutrient Composition Decide Harvest Maturity of Cucumis melo Varieties

Cucumis melo is a polymorphic taxon belonging to the family Cucurbitaceae, with several varieties based on ovary pubescence. Cucumis melo var. momordica and Cucumis melo var. acidulus are popularized and highly cultivated forms in South India. The characteristic of C.melo var.momordica is its fruit-cracking nature, which distinguishes the variety from others when it is ready to harvest. So, it would be a herculean task for the farmers to harvest ripened fruits before it gets cracked. Analysis of the ripening process's physicochemical attributes and nutritional composition is inevitable to understand and establish proper harvest management for the varieties. The current study was conducted in the experimental field of the Department of Botany, University of Kerala using two different melon varieties, Cucumis melo var. momordica (Roxb.) Duthie & Fuller (Snap melon) and Cucumis melo var. acidulus L. Naudin (culinary melon), from January to October 2021. Fruits were harvested at five developmental stages (S1: 5 days after pollination (DAP), S2: 10 DAP, S3:15 DAP, S4: 20 DAP, S5: 25 DAP) and analysed for physical and biochemical characters. Results were analyzed statistically by using one way ANOVA (P≤0.05). Pomological characteristics such as fruit weight and length at different developmental stages showed a tremendous peak from S3 to S5 in both varieties. However, the firmness of the fruits decreased from the S4 to S5 stage in varieties, reducing sugar accumulated sharply from the S2 to S3 stage. Titratable acidity content in Cucumis melo fruits continuously increased from the S1 to S5 stage. On the other hand, the total carbohydrate, cellulose, protein, and amino acid content increased from S1 to S2 but decreased sharply in S3 and S5. Ascorbic acid, total phenolics, lipid peroxidation, and electrolyte leakage levels declined with fruit ripening in Cucumis melo varieties. As a result of all the quality parameters mentioned above, Cucumis melo fruit harvested at the S4 maturity stage was the ideal harvest maturity for long-distance transportation and had higher consumer acceptability before fruit cracking. Our findings showed that the physical-biochemical properties and nutritional composition of Cucumis melo varieties change dynamically during ripening. The study highlighted the significance of maturity stages for fruit quality and provided critical information for optimal harvest management of the fruits of Cucumis melo varieties.

Panicle and Fruit Characteristics Influenced by Plant Age In Litchi cv. Rose Scented

An experiment was conducted during the cropping season of 2018 and 2019 to determine how plant ages affect flowering, fruit quality and incidence of sunburn and fruit cracking in litchi cv. Rose Scented. In a lot consisting of litchi plants aged between 1 and 30 years, 25-30- years-older plants initiated panicle emergence earlier with larger-sized panicles (42.35 cm length and 28.65 cm spread) and thicker peduncle (>6 mm). Fruit retention per panicle was also more in number (15.68) in 25-30- years older plants as compared to 1-5 year younger plants (4.76). An important observation involved fruits being borne closer to leaf in 25-30- years-older plants, which ultimately resulted in lesser incidence of sun burn and fruit cracking. Older plants (25-30-years) produced bigger fruit than 1-5 year-younger ones and highest fruit weight (22.58g) was recorded in 15–20-year-old plant. The time taken for fruit borne in older trees (25-30-years) to attain optimum fruit maturity was extended or delayed by 5 days as compared to younger plants (1-5-year). Total soluble solids was also higher in fruits borne on 25-30 years older litchi plants, while titratable acidity and ascorbic acid contents was more in fruits borne on 1-5 year younger plants. Total phenolics and antioxidant activity of pulp were the highest in young plants and least in older plants. we conclude that heavy bunches and proximity of fruit to leaf are important traits in litchi for consideration in breeding programmes to identify clones or variants tolerant to sun burn and fruit cracking. Bangladesh J. Bot. 53(2): 279-285, 2024 (June)

The β-galactosidase gene AtrBGAL2 regulates Akebia trifoliata fruit cracking

Cracking of Akebia trifoliata fruit at maturity is problematic for the cultivation of the horticultural crop, shortening shelf-life quality and compromising commercial value. However, the molecular mechanisms underlying this feature of A. trifoliata are not known. Genes involved in cell wall metabolism were identified by genome and transcriptome sequencing, which may play important roles in fruit cracking. One of the galactose metabolism related genes, β-galactosidase (AtrBGAL2), was identified in A. trifoliata, and overexpression (OE) of AtrBGAL2 resulted in early fruit cracking, higher water-soluble pectin contents, and lower acid-soluble pectin, cellulose, and hemicellulose content compared to the wild type. Whereas silencing of AtrBGAL2 in trifoliata by virus induced gene silencing showed opposite trends. The levels of AtrBGAL2 transcripts were 24.6 and 66.0-fold higher in OE A. trifoliata and tomato fruits, respectively, and the cell wall-related genes were also gradually greater than in control plants during fruit ripening. Whereas the expression levels of AtrBGAL2 was significantly down-regulated by 54.1 % and 73.7 % in gene silenced A. trifoliata and CRISPR/Cas9 tomato mutant plants, respectively, and cell wall-related genes were also significantly reduced. These results demonstrate that AtrBGAL2 plays important roles in regulating fruit cracking during fruit ripening.

Regulation of plum fruit cracking process during cold storage period by treatment of preharvest chitosan

Cracking fruit is a serious quality change phenomenon in the storage and transportation of postharvest plum fruit. To reduce the cracking rate of plum fruit during cold storage, the 'Guofeng No.7′ plum was sprayed with chitosan with 1.5% concentration 30d from preharvest. The postharvest plum fruit was stored at (0 ± 0.5) °C and (85–90) % relative humidity. The results showed that chitosan treatment could reduce the reactive oxygen species (ROS) level of plum fruit, effectively delaying the fruit cracking. Compared with the control, chitosan treated fruits inhibited the increase of free radical content such as O2−· and H2O2 and delayed the increase of membrane permeability and the accumulation of MDA (from 71.9 mmol/g to 53.5 mmol/g). It also regulates lipoxygenase (LOX), peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) (enzymatic activity from 34.15 g/min to 42.21 g/min) and ascorbate peroxidase (APX) activity and gene expression during fruit cracking to maintain membrane integrity and intracellular compartmentalization, thereby increasing the crack tolerance of plum fruit during cold storage. These results indicate that preharvest chitosan treatment can inhibit the metabolism of active oxygen species, increase the activity of ROS scavenging enzymes and gene expression, and delay the postharvest cracking of plum fruit.

Effect of preharvest biofilm application regimes on cracking and fruit quality traits in ‘0900 Ziraat’ sweet cherry cultivar

BackgroundFruit cracking impacts the quality of sweet cherry, significantly affecting its marketability due to increased susceptibility to injury, aesthetic flaws, and susceptibility to pathogens. The effect of 1% biofilm (Parka™) application regimes on fruit cracking and other quality parameters in the ‘0900 Ziraat’ cherry cultivar was investigated in this study. Fruit sprayed with water were served as control (U1). Fruit treated only once with biofilm three, two and one week before the commercial harvest were considered as U2, U3 and U4, respectively. Fruit treated with biofilm three, two, and one week before harvest were considered as U5; three and two week before harvest as U6; two and one week before harvest as U7; and fruit treated three and one week before harvest as U8.ResultsIn both measurement periods, the lower cracking index was obtained in biofilm-treated sweet cherry fruit. However, the firmness of biofilm-treated fruit was higher than that of the control fruit. The lowest respiration rate was observed in U7, while the highest weight was recorded in U4 and U5 than the control. The biofilm application decreased fruit coloration. The biofilm application also increased the soluble solids content of the fruit. The U2, U3 and U4 applications at harvest showed higher titratable acidity than the control. In both measurement periods, the vitamin C content of the U2, U5, U6, U7 and U8 applications was found to be higher than that of the control. The total monomeric anthocyanin of the U3 and U8 applications was higher than that of the control. Furthermore, the antioxidant activity of the U2, U3 and U5 in the DPPH, and the U7 and U8 in FRAP were measured higher thanthat of the control.ConclusionsThe application of biofilms has the potential to mitigate fruit cracking, prolong postharvest life of sweet cherries, and enhance fruit firmness.

Mitigating Fruit Cracking and Premature Dropping in Bael (Aegle marmelos) cv. NB-9 under Subtropical Conditions: Evaluating the Effects of Bio-formulations and Boric Acid

The present investigation entitled “Mitigating Fruit Cracking and Premature Dropping in Bael (Aegle marmelos) cv. NB-9 under Subtropical Conditions: Evaluating the Effects of Bio-formulations and Boric Acid” was carried out during the year 2022-2023 at Main Experimental Station, Department of Fruit Science, College of Horticulture & Forestry, A.N.D.U.A.&T., Narendra Nagar (Kumarganj), Ayodhya, Uttar Pradesh, India (Lat 26.540849° Long 81.830421°). Application of various bio-formulations and boric acid with eco-friendly materials to study the various vegetative properties, cracking, fruit drop and effect on the vegetative growth of fruit. The experiment was laid down in randomized block design (RBD) with (12) treatments and (03) replications. The experiment consists of 12 treatments including control, T1 (Control), T2 (FYM 50 Kg), T3 (FYM 100 Kg), T4 (Boric acid 200g), T5 (Boric acid 400g), T6 (Biofertilizer 250g/plant [100g Azotobacter + 100g Biovita + 50g PSB]), T7 (FYM 50Kg + Boric acid 200g), T8 (FYM 50Kg + Boric acid 400g), T9 (FYM 50Kg +Biofertilizer 250g/plant [100g Azotobacter + 100g Biovita + 50g PSB]), T10 (FYM 100Kg + Boric acid 200g), T11 (FYM 100 Kg + Boric acid 400g) and T12 (FYM 100Kg +Biofertilizer 250g/plant [100g Azotobacter + 100g Biovita + 50g PSB]) was used for this study. The results revealed that treatment T7 outperformed the rest with minimum fruit cracking (1.53%), fruit drop (76.20%), the maximum number of flowers per plant (171.19), day-to-flowering (31.38) and yield (181.69 q/ha) was most effective in a vegetative quality and yield point of view. Thus, the treatment combination T7 is therefore recommended for application to Bael trees in subtropical conditions to obtain high yields with better quality fruits.

Mechanisms driving fruit cracking in ‘Sinhwa’ pears (Pyrus pyrifolia Nakai) and effect of foliar fertilizer application on fruit quality

Fruit cracking poses a significant challenge to ‘Sinhwa’ pear cultivation and has potential economic repercussions for growers due to its adverse effects on yield and fruit quality. This study delves into the phenomenon of fruit cracking in ‘Sinhwa’ pears by conducting a comprehensive analysis of its causes and exploring the efficacy of foliar fertilization as a mitigation strategy. To reveal the conditions contributing to fruit cracking in ‘Sinhwa’ pears, moisture change regimes were systematically applied to 5-year-old ‘Sinhwa' trees from 2018 to 2020 during post-flowering and harvest periods. Alternating wet and dry conditions resulted in the highest fruit cracking rates, thus highlighting the importance of consistent soil moisture levels for mitigating cracking. Furthermore, the timing of fruit cracking and its histological characteristics in ‘Sinhwa’ pears were examined to understand the underlying causes. Microcracks appeared approximately 60 days after full bloom and preceded visible cracking, although notable changes in epidermis thickness and stone cell layer distribution were observed. Analysis of the nutrient content revealed differences in calcium and potassium levels between intact and cracked fruits, thus providing insights into potential nutritional influences on fruit quality. Potential mitigation strategies included foliar application of 0.3 % calcium chloride and 0.5 % potassium nitrate, and they demonstrated promising results in reducing fruit cracking rates compared to the control. Fruit characteristics, including mass and hardness, varied among the treatment groups, with treated trees showing notable increases in calcium content, which were correlated with decreases in fruit cracking incidence. To understand the molecular mechanisms underlying the effectiveness of foliar fertilization, gene expression levels associated with fruit cracking were analyzed by real-time PCR. Higher expression of heat-related expansion genes (Exp1 and Exp2) was observed in the treated fruits, particularly those in the calcium chloride and potassium nitrate treatments, suggesting the role of these elements in mitigating fruit cracking. This study provides valuable insights into the causes of fruit cracking in ‘Sinhwa’ pears and offers practical mitigation strategies, such as foliar fertilization. Moreover, the findings underscore the importance of maintaining consistent soil moisture levels and optimizing nutrient management practices to enhance fruit quality and minimize economic losses in ‘Sinwha’ pear cultivation.

Integrated transcriptomic, metabolomic, and functional analyses unravel the mechanism of bagging delaying fruit cracking of pomegranate (Punica granatum L.)

The economic impact of fruit cracking in pomegranate products is substantial. In this study, we present the inaugural comprehensive analysis of transcriptome and metabolome in the outermost pericarp of pomegranate fruit in bagging conditions. Our investigation revealed a notable upregulation of differentially expressed genes (DEGs) associated with the calcium signaling pathway (76.92%) and xyloglucan endotransglucosylase/hydrolase (XTH) genes (87.50%) in the fruit peel of non-cracking fruit under bagging. Metabolomic analysis revealed that multiple phenolics, flavonoids, and tannins were identified in pomegranate. Among these, calmodulin-like 23 (PgCML23) exhibited a significant correlation with triterpenoids and demonstrated a marked upregulation under bagging treatment. The transgenic tomatoes overexpressing PgCML23 exhibited significantly higher cellulose content and xyloglucan endotransglucosylase (XET) enzyme activity in the pericarp at the red ripening stage compared to the wild type. Conversely, water-soluble pectin content, polygalacturonase (PG), and β-galactosidase (β-GAL) enzyme activities were significantly lower in the transgenic tomatoes. Importantly, the heterologous expression of PgCML23 led to a substantial reduction in the fruit cracking rate in tomatoes. Our findings highlight the reduction of fruit cracking in bagging conditions through the manipulation of PgCML23 expression.