Crab Chitosan Research Articles

Valorization of freshwater crab shell residues to chitosan for postharvest quality of mango (Mangifera indica L.) by fruit coating

Freshwater crab shells (Somanniathelphusa dugasti) are abundant in inland areas and rich in chitosan, which can be extracted and utilized. This study investigated the characteristics of chitosan from freshwater crab shells (FC-chitosan) and its impact on extending the shelf life of Nam Dok Mai mangoes (Mangifera indica L.). The FC-chitosan had a high degree of deacetylation (99.15 %), thereby enhancing solubility and reactivity for a uniform coating, and a low ash content (0.75 %), minimizing the interference effects on mechanical strength and transparency, both of which could contribute to its potential use as a well-adhering coating material. The FC-chitosan film exhibited superior properties as a coating film, with relatively higher transparency (25.09 % T/mm), tensile strength (21.57 MPa), and elongation (76.09 %) compared to commercially available marine crab chitosan. Mango fruits coated with 2.0 % (w/v) FC-chitosan demonstrated appropriate postharvest quality, with lower weight loss and maintained peel color, fruit firmness, titratable acidity, and ascorbic acid during storage at room temperature (30 ± 5 °C) for up to 11 days. The results suggest that FC-chitosan is effective as a coating material for delaying ripening and extending the shelf life of mangoes. The novelty of using chitosan derived from freshwater crab shells lies in its unique properties and its sustainable use of underutilized byproducts.

Chitosan-melanin complex microsphere: A potential colonic delivery system for protein drugs

The characteristics and performance of chitosan-based colon delivery systems are significantly influenced by the method of preparation. Insect chitosan-melanin complex (CMC) may offer superior attributes over traditional shrimp and crab chitosan (CS) for colon-targeted administration. This study used dung beetle CMC as the carrier matrix and comprehensively examined the impact of various crosslinking techniques on the colonic drug delivery efficacy of microspheres, encompassing drug loading, swelling, drug release behavior, adhesion, enzymatic degradation, and absorption enhancement. The results indicate that F-TPPLC microspheres, crosslinked with a combination of formaldehyde and TPP, exhibit superior drug loading capabilities, optimal swelling behavior, and controlled in vitro drug release profiles in the colonic environment, along with excellent adhesion and enzymatic degradation properties within intestinal tract. Notably, these F-TPPLC microspheres increase paracellular permeability, possibly by disrupting the calcium-dependent adhesion junctions. In comparison to commercial CS, CMC demonstrates superior drug encapsulation efficiency, enhanced colonic drug release, adhesion, and absorption promotion, rendering it a favorable candidate as a carrier in colon-targeted drug delivery systems. Consequently, F-TPPLC microspheres derived from CMC are highly suitable for colon drug delivery applications and show promising potential for the oral delivery of peptide and protein-based therapeutics to the colon.

Chitosan extracted from Portunus sanguinolentus (three-spot swimming crab) shells: its physico-chemical and biological potentials

Aim: To summarize the extraction, physio-chemical, antibacterial and antioxidant properties of extracted chitosan from the exoskeleton of three-spot swimming crab, Portunus sanguinolentus as compared to commercial chitosan. Methodology: Chitosan biopolymer was extracted through demineralisation, deproteinization and deacetylation. The physico-chemical characterization of the extracted chitosan was carried out using Fourier Transforms Infrared Spectroscopy (FTIR), Field Emission Scanning Electron Microscopy (FESEM), X-Ray Diffraction (XRD), Thermogravimetric analysis (TGA) and Colorimetric analysis. Antibacterial activity using different concentrations of chitosan against Escherichia coli and Aeromonas hydrophila were tested by disc-diffusion method, while antioxidant activity was estimated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and H2O2 scavenging assay. Results: Crab chitosan (CC) showed the maximal zone of inhibition (17.00±0.50 and 15.16±0.577 mm), while commercial chitosan (CMC) showed 12.67±0.577 and 14.17±0.288 mm) against E. coli and A. hydrophila respectively. The scavenging potential of extracted chitosan ranged from 14 to 17% at different concentrations (0.05 to 0.2 mg ml-1). Crab chitosan efficiency to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals varied from 7.2 to 12.98% at different concentrations (0.05 to 0.2 mg ml-1). Interpretation: Overall, the extracted chitosan exhibited properties to commercial chitosan, suggesting that it could be utilized as a dietary supplement in the fishery sector to protect against bacterial infections. This circular economy approach could significantly benefit sustainable waste management and environmental health. Key words: Antibacterial, Biopolymer, Chitosan, Crab, Portunus sanguinolentus

The Use of Natural Polymers to Enhance Oil Recovery

This paper reports a laboratory study on the use of natural polymers to increase oil recovery. The use of natural polymers compared to synthetic polymers is more economical due to their abundant availability, renewability, biodegradability, and non-toxicity to the environment as they do not use chemicals. The purpose of this research is to measure the aqueous solution stability test and recovery factor produced by natural polymers through core flooding tests. The natural polymers used are Shrimp Chitosan, Crab Chitosan, Cyclea Barbata Miers, Mesona Palustris, and Seaweed. The methods used in this research are aqueous solution stability test and core flooding test. The shrimp chitosan solution used had a concentration from 5,000; 10,000 and 15,000 ppm and 2 salinities between 10,000 and 20,000 ppm. The crab chitosan solution used had a concentration from 5,000; 10,000 and 15,000 ppm with 2 salinities between 10,000 and 20,000 ppm. The Cyclea barbata Miers solution used had a concentration from 1,500; 2,500 and 3,500 ppm and 2 salinities between 7,000 and 15,000 ppm. The Mesona Palustris solution used had a concentration from 500; 1,000 and 2,000 ppm and 3 salinities from 10,000; 15,000 and 20,000 ppm. The Seaweed solution used had a concentration from 1,000; 2,000 and 3,000 ppm and 3 salinities from 5,000; 10,000 and 15,000 ppm. Sandpack is used as a porous media for crab chitosan experiments, while sandstone core is used for Cyclea Barbata Miers and Mesona Palustris with crude oil 44°API. The aqueous solution stability test showed that all polymer solution samples were clear for various concentrations and salinities. Except for the solutions of Cyclea barbata Miers and Masona palustris which are greenish and blackish respectively. Transmittance for shrimp and crab chitosan solutions shows a value of more than 80%. This shows that natural polymers are compatible with brine. Flooding tests were carried out to measure oil recovery related to the use of natural polymers. The experimental results showed that the best additional oil recovery was 10.3% using crab chitosan solution injection. The chitosan solution used had a concentration of 15,000 ppm and a viscosity of 1,729 cp. We can conclude that the addition of polymer can improve recovery factor.

Effect of Black Crab (Scylla Serrata) Chitosan Gel on the Three-Dimensional Socket Response and Fibroblasts after Tooth Extraction in Rattus Norvegicus

Effect of Black Crab (Scylla Serrata) Chitosan Gel on the Three-Dimensional Socket Response and Fibroblasts after Tooth Extraction in Rattus Norvegicus

Comparative Study on Flocculation Efficiency of Shrimp and Crab Chitosan for Irrigation-Ready Sludge

Comparative Study on Flocculation Efficiency of Shrimp and Crab Chitosan for Irrigation-Ready Sludge

High quality, low molecular weight shrimp and crab chitosans obtained by short-time holistic high-power microwave technology

The study sought to investigate the impact of a holistic high-power microwave technology during all stages of the extraction on the quality, time of extraction, and degree of deacetylation (DD) of shrimp chitosan (SC) and crab chitosan (KC). The demineralization and deproteinization stages took 7 and 8 min, at 750 and 875 W, respectively. The deacetylation process was conducted at two powers, 875 W and 1250 W, for 10, 15, and 20 min. It only took 25 min at 875 W to successfully prepare chitosan with a high DD and 30 min to reach the maximum DD. The highest DDs by the potentiometric titration method, FTIR, and 1H NMR of SC were 86.6%, 86.7%, and 83%, compared to 83.8%, 82.7%, and 80% for KC, respectively. Extracted SC had 79% solubility, 14.125 kDa, a 46.57% crystallinity index, 705.40% WBC, and 434.60% FBC, against 74.5%, 16.982 kDa, 74.14%, 689.82%, and 413.20% for KC, respectively. The study proved that 30 min of holistic high-power microwave at 875 W produced low-molecular-weight chitosan with relatively high deacetylation and low content of viscosity, crystallinity, and protein residue. The technique can provide a feasible alternative to the commercial production of low-molecular-weight chitosan in less time and energy.

The Potential of Crab Chitosan Polymer as EOR Injection Fluid

The abundant availability of crab shell waste in Indonesia is still not optimally utilized. Crab shell contains chitin that can be processed into chitosan. Chitosan polymer can be developed to be utilized on Enhanced Oil Recovery. The objective of this study is to measure the amount of biopolymer adsorption potential of crab shell on sandstone both statically and dynamically, in order to obtain oil recovery potential by using crab shell chitosan as injection fluid. Method of this study is categorized as experimental research through direct observation inside a laboratory. The utilized biopolymer in this research was chitosan made from crab shell. Biopolymer solution concentrations in this research are 5,000, 10,000, and 15,000 mg/L and the formation water salinity levels were at 10,000 and 20,000 mg/L. The research was conducted by using variations of formation water compounds namely NaCl and MgCl2. The research conducted consists of viscosity, transmittance, and adsorption measurements on biopolymer and water formation salinity concentrations. The sand pack flooding was conducted to observe oil displacement by selected formation water and chitosan solution. After conducted viscosity test, solution with the highest viscosity levels were chosen as driving fluid in sand pack flooding. The solutions are N2R3 samples which are crab shell chitosan solution with formation water containing NaCl with salinity level 20,000 mg/L and chitosan concentration of 15,000 mg/L. The viscosity value of the sample was at 1728.67 cP. Conclusion: Crab chitosan with 15,000 mg/L concentration, 20,000 mg/L salinity, and viscosity value of 1728.67 cp can be optimally utilized in sand pack flooding by providing incremental recovery factor of 10.27%.

Use of Various Types of Chitosan as Edible Coating to Maintain Physical Quality of Tomatoes (Solanum lycopersicon L.) During Storage at Two Different Temperatures

Increasing tomato production requires special handling to increase longer shelf life by applying chitosan and modifying storage temperatures. Chitosan is a chitin derivative whose function is similar to wax compounds so that it is able to inhibit metabolic activity. Storage temperature of 10°C can extend shelf life and maintain the physical quality of tomatoes. This study aims to determine the effect of chitosan administration, the effect of storage temperature, and the effect of the combination of chitosan and storage temperature on the physical quality of tomatoes. This study used a Factorial Complete Randomized Design, the first factor was tomatoes without chitosan coating (K0), and tomatoes with chitosan coating (K1) = shrimp shell, (K2) = crab shell, (K3) = fish scales using the same concentration of 6%. The second factor is storage temperature (T1) = room temperature (26-32°C) and (T2) = temperature 10°C. The changes observed are shrinkage in weight, color, texture, aroma, taste based on organoleptic test (hedonic test). Data analysis using ANOVA at 5% level and BNJ follow-up test at 5% level. The results showed that the use of crab chitosan (K2) had an effect on weight loss (0.42%). Storage temperature of 10°C (T2) affects weight loss (5.04%). The best treatment combination for weight loss (0.07%) and color (7.40) was crab chitosan and temperature of 10°C (K2T2), while the best treatment combination for texture (7.56), aroma (7.08), and taste (7.16) was fish scales and temperature of 10°C (K3T2). In general, the best treatment is chitosan fish scales and a temperature of 10°C (K3T2).

Chitin and chitosan extraction: A comparison of three crab species from fresh, brackish and marine water environments

Chitin was extracted from three species of crab obtained from biowastes of marine crab (Portunus. pelagicus), brackish water crab (Scylla sp.), and freshwater crab (Gecarcinucidea sp.). The chemical isolation involved 1 M NaOH and 1 M HCl for demineralization and deproteinization, respectively. 50 % NaOH deacetylated chitin to chitosan at 100 °C for 2 h. Highest chitin yield (15.11 %) was obtained from freshwater crab while no significant difference existed between brackish and marine. Chitosan from freshwater exhibited lowest ash content but highest solubility (65.94 %) and DDA (81.17 %). FTIR revealed functional groups peculiar to chitosan and further confirmed its α – group. XRD, SEM as well as bulk and tapped densities showed the smooth and porous nature of the chitosan. It was concluded that the variability of crabs' environment affected their shell formation and development which further impacted on chitin deposit. High solubility and DDA of freshwater crab chitosan makes it suitable as coagulant.

Comparison of the ameliorative roles of crab chitosan nanoparticles and mesenchymal stem cells against cisplatin-triggered nephrotoxicity

AimIn the present investigation, we compared the effects of mesenchymal stem cells extracted from bone marrow (BMSCs) and crab chitosan nanoparticles (CCNPs) on renal fibrosis in cisplatin (CDDP)-induced kidney injury rats. Material and methods90 male Sprague-Dawley (SD) rats were divided into two equal groups and alienated. Group I was set into three subgroups: the control subgroup, the CDDP-infected subgroup (acute kidney injury), and the CCNPs-treated subgroup. Group II was also divided into three subgroups: the control subgroup, the CDDP-infected subgroup (chronic kidney disease), and the BMSCs-treated subgroup. Through biochemical analysis and immunohistochemical research, the protective effects of CCNPs and BMSCs on renal function have been identified. ResultsCCNPs and BMSC treatment resulted in a substantial rise in GSH and albumin and a decrease in KIM-1, MDA, creatinine, urea, and caspase-3 when compared to the infected groups (p < 0.05). ConclusionAccording to the current research, chitosan nanoparticles and BMSCs may be able to reduce renal fibrosis in acute and chronic kidney diseases caused by CDDP administration, with more improvement of kidney damage resembling normal cells after CCNPs administration.

Chitosan/collagen-based hydrogels for sustainable development: Phycocyanin controlled release

Marine biopolymers obtained from fishery by-products, such as chitosan and collagen, are sustainable alternatives to developing eco-friendly hydrogels. This work focused on the development of composite hydrogels based on blue crab chitosan (Cs) and bluefin tuna collagenous protein (BTCP), at different concentrations (10%, 20%, and 50%; w/w Cs), by using a freezing/thawing approach. Cs-BTCP composite hydrogels were characterized in terms of structural, morphological, thermal, mechanical, rheological, and antioxidant properties. A highly interconnected porous structure is shown for all the freeze-dried composite chitosan hydrogels by SEM imaging. Additionally, by increasing the concentration of BTCP, the porosity, and swelling degree of the composite hydrogels were improved. Data also revealed that BTCP addition enhanced the elasticity and mechanical resistance of composite hydrogels, especially HG prepared with 50% of BTCP, which explain its high swelling ability which reaches 2160% compared to 1396% for HG-C, at pH 5.5. HG-50 was selected for the in vitro release study of phycocyanin as a bioactive molecule and the results revealed that the release depends on the medium pH. Kinetic studies show that phycocyanin release is mainly governed by drug diffusion. Therefore, pH-sensitive Cs-BTPC composite hydrogels could provide an appropriate and supportive microenvironment for the loading and administration of drugs.

Extraction and characterization of chitosan from Eupolyphaga sinensis Walker and its application in the preparation of electrospinning nanofiber membranes

Due to its capabilities for wound healing, antimicrobial defense, hemostasis, and biodegradation, chitosan has seen increased use in biomedical disciplines in recent years. In the meantime, efforts have been made to develop and use insect chitosan as a source to address the seasonal, irritating, and regional shortcomings of traditional shrimp and crab chitosan. In this study, a new type of insect chitosan (DCS) was first extracted from Eupolyphaga sinensis Walker by a low-temperature intermittent method and was compared with commercially available pharmaceutical chitosan (CS). Firstly, the degree of deacetylation and molecular weight of DCS were determined, and DCS was characterized by FT-IR, 1H NMR, XRD, and TGA-DTG. On this basis, DCS was mixed with PVA and PEO to create a novel electrospun nanofiber membrane. The air permeability, antibacterial properties, and biocompatibility of the nanofiber membrane were evaluated, as well as the membrane's shape, structure, and mechanical characteristics. Finally, the activity of nanofiber membranes in promoting wound healing was verified with a rat full-thickness skin defect model, hoping to provide a reference for the development of new drug delivery carriers and wound dressings.

Enhanced photocatalytic and antibacterial activity of ZnO with rice field crab chitosan and plectranthus amboinicus extract

Recently, there has been considerable interest in the development of hybrid nanomaterials for health and photocatalytic applications. This study describes the ecofriendly strategy for synthesis of zinc oxide (ZnO) nanoparticles immobilized chitosan (CS) obtained by deacetylation of rice field crab Paratelphusa hydrodromous shells in presence of Plectranthus amboinicus leaf extract (PLE). The resulting CS-ZnO-PLE hybrid nanocomposites were characterized by FT-IR, XRD, UV–Vis-DRS, HR-SEM, TEM, TGA and BET analysis. The HR-SEM study reveals that the CS-ZnO shows hexagonal structure after intercalation of PLE, their structure changed to hierarchical flower-like structure. Presence of reactive functional groups in the chitosan and phytochemicals such as thymol, carvacrol, p-cymene, β-caryophyllene, α-pinene, β-pinene, 1,8-cineole etc in the leaf extract plays a major role in the stabilization of the CS-ZnO-PLE hybrid nanocomposite. Spectroscopic findings reveal that photon energy from sunlight induces an electron that can be transferred from photoexcited chitosan to the conduction band of ZnO NP, which induces the production of reactive oxygen species (ROS) such as H2O2, ●OH and ●O2− are responsible for effective antibacterial and photocatalytic activities. The CS-ZnO-PLE hybrid nanocomposite shows enhanced photocatalytic degradation on methylene blue (∼98.97%) and drimarene red (∼87.43%) than CS-ZnO 67.53% and 30.46% respectively in the duration of 1 h exposure. The antibacterial activity was investigated in terms of inhibition zones using S.aureus and E.Coli bacterial strains and their inhibition zones were found to be 21 and 20 nm. These results provide valuable information for development of alternative chitosan material from rice field crab shells for high efficient photocatalytic and antibacterial activity with metal oxides.

Effect of invasive blue crab chitosan coating on extending the shelf life and quality of fresh strawberry fruits

The diversity of marine biomasses is a set of exploitable and renewable resources with application in several sectors. The blue crab was considered as a threat by the Tunisian fishermen in 2014, since it induces several damages on nets but also on captures. In addition, in 2017, the Tunisian State launched a plan to exploit and promote this species of crab. In the context of valuation, the chitin was isolated by a conventional chemical method from the shell of blue crabs. The efficiency of chitin extraction from Callinectes sapidus (C. sapidus) was achieved with a yield of 27.6 %.Then; the chitosan was prepared by N-deacetylation of chitin with a yield of 16 %, leading to a degree of acetylation (DA) of 89.9%. For Portunus segnis (P. segnis) the yield of chitin was 31.2% while the chitosan yield was 20% and the degree of acetylation was 79.9% After these analyzes, it can be deduced that chitosan has a high degree of purity and that it is considered effective for the preservation of strawberries in favor of its antioxidant and antimicrobial capacity.

EFFECT OF CHITOSAN AND SODIUM CHLORIDE APPLICATION ON THE STORAGE STABILITY OF COOKED WALNUT SEEDS

Samples of cooked walnut (Tetracarpidium conophorum) seeds were subjected to proximate and microbiological analysis. The nuts were divided into eight groups and subjected to various preservative hurdles for 20 days. Proximate analysis showed that the nut had moisture, lipid, protein, ash, carbohydrate and crude fibre content of 46.70±1.50, 6.00±0.05, 53.53±2.31, 2.96±0.06, 32.79±1.50 and 4.72±0.15 respectively. Microbiological analysis showed the presence of bacteria such as Bacillus subtilis, Staphylococcus aureus, Leuconostoc spp, Pseudomonas spp and Escherichia coli. Fungi isolates include Saccharomyces cerevisiae, Candida spp, Aspergillus niger and Aspergillus flavus. Among the bacteria, Staphylococcus aureus, and Leuconostoc spp had the highest percentage (100%) occurrence. Saccharomyces cerevisiae was predominant (80%) among the fungi. Sample preserved with sodium chloride and stored at 4±10C had the least microbial count (3.00log10cfu/g) compared to others. The best preservative hurdle in the study was the application of 1000?g/g of chitosan for 2 weeks. Chitosan preserved walnut not only remained fresh but had chemical composition like the freshly cooked sample. The results show that Tetracarpidium conophorum is a good source of protein for man and animals as it contains nutrients that are necessary for maintenance of body cells and tissues. Successful shelf-life extension can be achieved using crab chitosan

Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from Hermetia illucens Relative to Crab Chitosan.

This study shows the research on the depolymerisation of insect and crab chitosans using novel enzymes. Enzyme preparations containing recombinant chitinase Chi 418 from Trichoderma harzianum, chitinase Chi 403, and chitosanase Chi 402 from Myceliophthora thermophila, all belonging to the family GH18 of glycosyl hydrolases, were used to depolymerise a biopolymer, resulting in a range of chitosans with average molecular weights (Mw) of 6–21 kDa. The depolymerised chitosans obtained from crustaceans and insects were studied, and their antibacterial and antifungal properties were evaluated. The results proved the significance of the chitosan’s origin, showing the potential of Hermetia illucens as a new source of low molecular weight chitosan with an improved biological activity.

Screening factors affecting chitosan extraction from mud crab (Scylla sp.) shell using microwave irradiation for the Response Surface Approach

Extracting chitosan from crab shell using conventional method requires processing in strong acid and alkali conditions under high temperature with long reaction time. Microwave-based extraction proposes shorter processing time, and hence energy and cost efficient. This study intended to screen factors affecting the extraction of chitosan from mud crab shell using microwave irradiation. The factors would be used in Response Surface Methodology (RSM) approach to obtain the optimum conditions for chitosan extraction. Microwave irradiation was employed in all of the three steps of chitosan extraction, the demineralization, deproteination, and deacetylation processes. A fractional factorial design was used to screen eight independent factors to determine the most significant ones to be optimized to determine the maximum value of four responses, which were yield, mineral removal, degree of deacetylation and moisture content. The results show that all of the main independent variables were significant in affecting minimal one of the 4 responses (P < 0.05). Mud crab chitosan had the degree of deacetylation of 87.72 – 95.13 %. Results of the screening analysis concluded, the main independent factors that are going to be applied in the optimization study are NaOH concentration, microwave oven power, and reaction time in deacetylation process.

The Physicochemical Characteristics of Smart Food Bars Enriched with Moringa Leaf Extract And Chitosan as An Emergency Food in Disaster Times

Emergency food products are processed food specifically designed to meet human daily needs of at least 2500 Kcal/day. One of the emergency food products, namely food bars, is a high-calorie food product, enriched with other nutrients and produced in solid form. The packaging of food bars designed in a small size makes their distribution relatively easy. The purpose of this study was to characterize the emergency food products of smart food bars by utilizing local food ingredients, including flour, mackerel fish flour, moringa leaf extract, and coconut crab chitosan at various concentrations. Furthermore, it delved into the formulation and physicochemical characterization of the best smart food bars as an emergency food by substituting cassava flour and skipjack tuna flour enriched with moringa leaf extract and coconut crab chitosan. This research used a completely randomized factorial design by combining treatments with cassava flour and skipjack fish flour as well as a combination of treatment with moringa leaf extract and the addition of coconut crab chitosan (0%, 5%, 10%, 15% 20%). The results showed that the N3 treatment (15% moringa leaf extract and chitosan) with a texture value of 2,768 N/m, color values (L*, a*, b*) were 63.35, 5.30, and 11.91, respectively. The findings also highlighted 13.29% protein, 17.80% fat, 6.55% water, 0.96% ash, 60.76% carbohydrates, 7.41% antioxidants, and total calories of 230.57 kcal. The study has concluded that the use of moringa leaf extract and coconut crab chitosan can enhance the characteristics of smart food bars as emergency food during disasters.

γ-Irradiated Chitosan From Carcinoscorpius rotundicauda (Latreille, 1802) Improves the Shelf Life of Refrigerated Aquatic Products

The γ-irradiated horseshoe crab chitosan was used as food coating to extend the shelf life of marine shrimp and fish. Fourier-transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FE-SEM), and X-ray diffraction (XRD) were used to characterize the γ-irradiated chitosan. After employing control (untreated seafood samples/no preservatives), chemical preservation (treated with 2% glacial acetic acid) and 2% chitosan (0, 10, and 20 kGy γ-irradiation) for the assessment assay, the shrimp (Penaeus merguiensis), pomfret (Pampus argenteus), and hilsa fish (Tenualosa ilisha) samples were examined for pH, thiobarbituric acid reactive substance (TBARS), total viable counts (TVC), and sensory evaluation changes while under 15-day refrigeration at 4°C. The results of FT-IR, XRD, and FE-SEM analysis revealed that irradiated chitosan possessed a crystalline structure with smooth texture on its surface. Analysis of pH, TBARS, TVC, and sensory evaluation demarcated irradiated chitosan with the ability to delay microbial growth and this prolonged the shelf life of refrigerated shrimp and fish. With novelty on γ-irradiated horseshoe crab chitosan use as natural preserving agent, fisheries industries and food packaging practitioners would benefit from its microbial-inert abilities particularly for long distant cold storage transport of packaged marine meats.