Cottonseed Research Articles

An Analysis of Marketing Status of BT Cotton Seed in Devbhumi Dwarka District, Gujarat, India

The main objectives of the study were to assess the company’s distribution pattern of quality seed material their market share, marketing channels. Multistage sampling technique were adopted. The total 120 farmers and 20 dealers were selected from four taluka i.e., Bhanvad, Khambhalia, Kalyanpur, Okhamandal (Dwarka) of Devbhumi Dwarka district of Gujarat state. The five wholesaler/distributors were selected from district headquarters. The primary data were collected through personal interview with farmers, dealers, wholesaler/distributors using well-structured questionnaires and interview scheduled. Results revealed that most of Bt cotton growers to be middle aged farmers. The pattern of land holding distribution shows that majority of the Bt cotton growers belonged to the small and marginal land holding category. Social participation of Bt cotton growers shows that they are not members of any organization. The well was found to be the main source of irrigation water and most of Bt cotton growers were reported to attained primary level of education From the study, it was found that Rasi seed (P) Ltd. Occupied major market share in Devbhumi Dwarka district. Among different marketing channels, it was observed that marketing channel-I (MC-I) (Company→ Wholesaler/Distributors→ Dealer→ Bt cotton grower) is the most prevalent channel in the sampling area.

Development of an Environmentally Friendly, Economical and Effective Method for Pre-sowing Treatment of Cotton Seeds with a Pulsed Electric Field

The aim of this work is to study the effect of electric pulse gas discharge on cotton seeds in pre-sowing processes in grass-grass-gray soils. The studies were conducted on cotton seed samples brought from the Saatli district of the Azerbaijan Republic. Cotton seed samples were treated with pulse gas discharge at 14 kV for 15 minutes at atmospheric pressure and room temperature. As a result of agrotechnical measures in cotton agrocenosis, the living conditions of microorganisms change dramatically. The number of microorganisms increases. Processing seed in the electric field of pulse discharge helps to reduce the number and harmfulness of phytophages by 46.5-68.3% and 13.1-88.5%, respectively.

GhDOFD45 promotes sucrose accumulation in cotton seeds by transcriptionally activating GhSWEET10 expression.

Cotton seed development and fiber elongation are the inseparable and overlapped development processes requiring the continuous supply of sucrose as the direct carbon source. However, little is known about the molecular mechanism of how sucrose is transported from the source tissues (leaves) into growing cotton seeds. Here, we identify the function of a sucrose transporter gene, Sugars Will Eventually be Exported Transporter 10, GhSWEET10 in cotton seed development. GhSWEET10 encodes a functional sucrose transporter, predominantly expressing in the funiculus, inner seedcoat, and endosperm during fiber elongation. GhSWEET10 RNAi plants (GhSWEET10i) accumulated less sucrose and glucose in growing seeds and that led to shorter fibers and smaller seeds, whereas GhSWEET10 overexpressed plants (GhSWEET10OE) had bigger seeds and longer fibers with more sugar accumulation during fiber elongation. GhSWEET10 gene is transcriptionally controlled by the transcription factor GhDOFD45. GhDOFD45 knockout plants (GhDOFD45-KO) possessed the phenotypes of smaller seeds and shorter fibers like those of GhSWEET10i plants. Furthermore, GhSWEET10 mainly exports the sucrose from the funiculus into developing seeds according to the mimic-analysis of sucrose transporting. Collectively, all these findings show that GhDOFD45 positively regulates GhSWEET10 expression to mainly transport sucrose from leaves into developing cotton seeds. Our findings also imply that the sucrose transport into enlarging seeds benefits fiber development, and thus GhSWEET10 can be selected as a target of breeding novel cotton varieties with larger and more vigorous seeds.

Constraints Faced by Bt Cotton Seed Companies in Gujarat: A Comprehensive Analysis

India's cotton industry is crucial to the economy, providing raw material for 75% of the textile industry and employing millions. Despite having the largest area under cotton cultivation globally, India faces challenges such as low productivity due to rain-fed farming. To address these challenges by enhancing productivity and pest resistance the Bt cotton producer companies were facing different constraints. Data from various stakeholders in Gujarat's Bt cotton seed market was collected and analysed using the Garrett Ranking Method to identify key factors influencing sales. The findings highlight the major constraints faced by Bt cotton producer companies includes the fixed price policy, lack of skilled manpower and seed adulteration. By improving access to quality seeds, enhancing farmer awareness and reforming seed policies, which significantly helps market dynamics in shaping the cotton seed sector.

Water fluxes and nutrient absorption along the midgut of three hemipterans, Mahanarva fimbriolata, Dysdercus peruvianus, and Rhodnius prolixus

Hemiptera Order comprises insect species adapted to different diets regarding water and nutrient content and availability, thus suggesting different combinations of proteins to ensure their absorption. To find out whether hemipterans use the same or distinct set of proteins and whether these differences are related to the phylogeny or the diet, RNAseq analyses were conducted in gut sections of three hemipterans, M. fimbriolata, D. peruvianus, and R. prolixus, with remarkable distinct diet. Since only a few of the selected proteins were functionally characterized, the coded putative proteins were manually curated by bioinformatics to infer their physiological function. The results suggest a relationship between gene expression patterns and water and nutrient dietary content and availability. In contrast, putative gene expansions and deletions are related to phylogeny, corresponding to evolutionary adaptations of ancestral forms to feed on xylem, cotton seeds, and blood, resulting in more resemblances between D. peruvianus and R. prolixus than M. fimbriolata. M. fimbriolata absorbs water through aquaporins Drip and Prip in the filtration chamber by passive diffusion, with a higher contribution of water-selective Drip. D. peruvianus water absorption involves Drip and Prip, but Prip contribution appears to be higher, and they probably cooperate with water-ion cotransporters in the posterior midgut. R. prolixus absorbs water in the anterior midgut involving a sodium transporter and a putative water-urea Prip. Sugars, amino acids, and lipids might be absorbed along the midgut in the three species, with a higher contribution of the posterior midgut for amino acid and lipid absorption in M. fimbriolata and D. peruvianus and the middle midgut in R. prolixus.

Comparative Transcriptome Analysis of Gossypium hirsutum Mutant (xin w 139) and Wild-Type (Xin W 139) Plants During Seed Embryo Development

Background: Cotton seeds are the main byproduct of cotton crops. The phenomenon of plants failing to develop mature and full seeds is called seed embryo abortion, which leads to a decrease in seed yield and potentially causes economic losses. Methods: We report a phenotypic evaluation of seed embryos from G. hirsutum mutant (xin w 139) and wild-type (Xin W 139) lines and a comparative RNA-seq study at four developmental stages. Results: The field results from two years showed that the sterility rate and malformation rate of xin w 139 were significantly lower than those of Xin W 139, and the RNA-seq data revealed that the differences in the development of the seed embryos of the two lines mainly occurred after 20 days post anthesis (DPA). Differential analysis revealed a total of 29,151 differentially expressed genes (DEGs), including 2696 transcription factors (TFs), between the two lines, in which the fatty acid and glucose metabolism-related pathways were significantly enriched. These DEGs were divided into 8 clusters, with the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of each cluster being annotated. Furthermore, a gene regulatory network was built using weighted correlation network analysis (WGCNA), revealing 9 key genes that play crucial roles in shaping the developmental disparities of seed embryos between the two lines, among which 3 are TFs. Conclusions: These findings offer a foundational framework for comprehending the molecular mechanisms underlying cottonseed embryo development, as well as presenting novel genetic reservoirs for further investigations into cottonseed embryo development.

Evaluating the Performance of Agricultural Tractor Engine using Cottonseed Oil Biodiesel

The energy demand has been significantly increasing around the world in the last century. Conventional fossil fuels as one of the sources has been highly demanded due to its negative environmental effect. The growing demand for energy stimulated the development of renewable and eco-friendly alternative energy sources. Produced from vegetable oils and animal fats, biodiesel is a renewable, sustainable, and biodegradable fuel. It can be used as an alternate fuel to conventional diesel (CD) in compression ignition (CI) engines. The Belarus 920 tractor, with a maximum output of 74.5kW, was put through a Power Take Off (PTO) test in this study to assess its performance and emission components when fueled with conventional petroleum diesel, B20, B50, and B100 of Cotton Seed Oil Biodiesel (CSOME). The performance results showed that the power and Break Thermal Efficiency (BTE) fell, while the BSFC improved, when the tractor was fueled with neat CSOME and its mixes.

Nano-enhanced storage of American cotton using metal-oxide nanoparticles for improving seed quality traits.

Cotton seeds have poorer germination than other crops because of their high sensitivity towards insect pests and other biotic and abiotic stresses during the germination process. In the present study, inorganic bulk and nano nutrients of zinc oxide (ZnO) and titanium oxide (TiO2) nanoparticles were synthesized using the chemical reduction method and invigourated with cotton seeds. The characterization of nanoparticles was done by FESEM, HRTEM, UV/Vis analysis and FTIR. The delinted and fuzzy seeds of two American cotton varieties (H 1300 and H 1098-i) were nano-primed for 10h with zinc oxide nanoparticles (ZnONPs) @ 400ppm and titanium dioxide nanoparticles (TiO2NPs) @ 100ppm. After nanoparticle invigouration, the seeds were analyzed for various parameters at different intervals (0months, 3months, 6months, 9months and 12months) such as germination percentage, seedling length, seedling dry weight, electrical conductivity, dehydrogenase activity, antioxidant enzyme activity. The results indicated that that different storage periods and nanopriming treatments had significant effects on all seed quality parameters except the effect of nanopriming treatments on germination percentage (excluding delinted seeds of H 1098-i). It is also revealed that the interaction effect of nanopriming treatment and storage period was non-significant on all parameters except EC. Maximum reduction in seed quality parameters was observed in control treatment and minimum was found when seeds were nanoprimed with ZnONPs @ 400ppm. The differences in the response for both NPs can be attributed to their surface charge, and concentration used. Overall, ZnONPs and TiO2NPs could hold seed quality and vigour during the storage of cotton seeds of American varieties (H 1300 and H 1098-i).

Impact of dielectric barrier discharge plasma and plasma-activated water on cotton seed germination and seedling growth

Unfavorable environmental conditions during planting can reduce seed germination and hinder seedling growth. To address this issue, manufacturers are exploring innovative and cost-effective methods, such as cold plasma discharge. This simple, low-cost, and efficient physical technique induces significant biological responses in seeds and plants without the use of traditional, environmentally hazardous chemicals. This study investigated the impact of dielectric barrier discharge (DBD) plasma and plasma-activated water (PAW), produced by gliding arc plasma, on the germination and seedling growth of My344 cotton seeds. The seeds were pre-treated with 80 W of DBD plasma for 0, 1, 2, and 3 min, and subsequently soaked for 90 min in PAW with varying pH levels of 5.82, 3.88, 3.63, and 3.38. The results showed that plasma treatment positively influenced seed germination and seedling growth. The highest germination percentage (98.89 %) was observed with 1 min of DBD treatment, followed by PAW priming at pH levels of 3.63 and 3.38. Additionally, a 3-min DBD treatment followed by soaking in PAW with a pH of 3.63 led to significant increases in stem length (76.76 %), root length (48.77 %), and wet weight (76.44 %). Furthermore, it was observed that the electrical conductivity of the seeds in all groups decreased significantly with increased PAW acidity. The physical and chemical effects of cold DBD plasma on the seed surface, as well as changes in hydrophilicity, were further examined using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and water contact angle imaging.

Transcriptome Analysis and Identification of Genes Associated with Cotton Seed Size.

Cotton seeds, as the main by-product of cotton, are not only an important raw material for edible oil and feed but also a source of biofuel. The quality of cotton seeds directly affects cotton planting and is closely related to the yield and fiber quality. However, the molecular mechanism governing cotton seed size remains largely unexplored. This study investigates the regulatory mechanisms of cotton seed size by focusing on two cotton genotypes, N10 and N12, which exhibit notable phenotypic variations across multiple environments. Developing seeds were sampled at various stages (5, 20, 30, and 35 DPA) and subjected to RNA-seq. Temporal pattern clustering and WGCNA on differentially expressed genes identified 413 candidate genes, including these related to sugar metabolism that were significantly enriched in transcriptional regulation. A genetic transformation experiment indicated that the overexpression of the GhUXS5 gene encoding UDP-glucuronate decarboxylase 5 significantly increased seed size, suggesting an important role of GhUXS5 in regulating cotton seed size. This discovery provides crucial insights into the molecular mechanisms controlling cotton seed size, helping to unravel the complex regulatory network and offering new strategies and targets for cotton breeding to enhance the economic value of cotton seeds and overall cotton yield.

Performance and Emission Characteristics of VCR Engine Fueled with TYRE Oil and Cotton Seed Oil Blends

Performance and Emission Characteristics of VCR Engine Fueled with TYRE Oil and Cotton Seed Oil Blends

Analysis of influencing factors of raw cotton quality and prospect of optimisation pathway

The paper discusses the issue of raw cotton quality and summarises it in the context of the current state of research. The article highlights the importance of cotton seeds for improving cotton quality and points out the problems in cotton seed selection and breeding. The article also mentions other factors affecting cotton quality, such as planting technology, light factors, defoliation and ripening technology, anisotropic fibre content and cotton picker operation quality. Based on these findings, ways to optimize the quality of raw cotton are proposed, including exploring suitable cotton varieties for machine picking, mitigating differences in raw cotton quality due to differences in geographic location and climatic conditions, and optimizing the use of picking techniques and processing machinery to reduce the impact on raw cotton quality. In conclusion, the paper provides a useful reference for promoting the application of cotton mechanical picking technology.

Low temperature exposure influences nitrogen metabolism resulting in decreased Cry1Ac insecticidal endotoxin content in cotton seeds

BackgroundSudden temperature drops, resulting from extreme weather events, often occur during the boll-setting period of cotton in Xinjiang, China, causing decreased expression of Bacillus thuringiensis (Bt) insecticidal proteins in cotton bolls. The precise threshold temperatures and durations that lead to significant changes in Cry1Ac endotoxin levels under low temperatures remain unclear. To address this, we investigated the effects of different temperatures and stress durations on Cry1Ac endotoxin levels in cotton bolls. In 2020–2021, two Bt transgenic cotton varieties, conventional Sikang1 and hybrid Sikang3, were selected as experimental materials. Various low temperatures (ranging from 16 to 20 °C) with different durations (12 h, 24 h and 48 h) were applied during the peak boll-setting period.ResultsAs the temperature decreased, the Cry1Ac endotoxin content in the boll shell, fiber, and seed exhibited a declining trend. Moreover, the threshold temperature which caused a significant reduction in Cry1Ac endotoxin content increased with the prolonged duration of low-temperature stress. Among the components of cotton bolls, seeds were most affected by low-temperature stress, with the threshold temperature for a significant reduction in Cry1Ac endotoxin content ranging from 17 °C to 19 °C. Correlation analysis indicated that low temperatures led to a decrease in protein synthesis capacity and an increase in degradation ability, resulting in reduced Cry1Ac endotoxin content. Pathway analysis revealed that both free amino acid and peptidase had significant negative effects on Cry1Ac endotoxin content.ConclusionIn summary, when the daily average temperature was ≤ 19 °C, implementing cultural practices to reduce free amino acid content and peptidase activity could serve as effective cold defense strategies for Bt cotton production.

Mammary gland responses to altering the supply of de novo fatty acid substrates and preformed fatty acids on the yields of milk components and milk fatty acids

The objective of our study was to evaluate the effect of altering the dietary supply of acetate, palmitic acid (PA), and cottonseed on the yields of milk components and milk fatty acids (FA) in lactating dairy cows. Thirty-two multiparous Holstein cows (133 ± 57 d in milk, 50.5 ± 7.2 kg/d milk) were used in a 4 × 4 Latin square split plot design with a 2 × 2 factorial arrangement of subplot treatments. Cows were blocked by energy-corrected milk (ECM) yield and allocated to a main plot receiving a basal diet (n = 16) with no supplemental PA (Low PA) or a basal diet (n = 16) with 1.5% inclusion of a FA supplement containing ~85% PA (High PA). In each main plot, the following subplots of treatment diets were fed in a Latin square arrangement consisting of 14-d periods: 1) a control diet (CON), 2) the control diet supplemented with 3% sodium acetate (AC), 3) the control diet supplemented with 12% whole cottonseed (CS), and 4) the control diet supplemented with 3% sodium acetate and 12% whole cottonseed (CS+AC). The PA supplement and sodium acetate replaced soyhulls, and whole cottonseed replaced cottonseed hulls and meal. All diets were balanced for 30% neutral detergent fiber (NDF), 23% forage NDF, 28% starch, and 17% crude protein (CP). Sources of FA were classified as de novo (<16 carbons), mixed (16-carbon), and preformed (>16 carbons). The statistical model included the random effect of cow nested within basal diet and fixed effect of period, basal diet, acetate, cottonseed, and their interactions. Three-way interactions among basal diet, acetate, and cottonseed were observed for the yields of milk fat, 3.5% fat-corrected milk (FCM), and the molar yields of de novo FA, mixed FA, and preformed FA. In the Low PA diets, AC and CS+AC increased the yields of milk fat and FCM compared with CON and CS, whereas, in the High PA diets, CS+AC increased the yields of milk fat and FCM compared with the other treatments and AC increased milk fat yield compared with CON and CS. Compared with Low PA, High PA increased milk fat content, mixed FA yield, and tended to increase C4:0 yield. Diets containing acetate increased DMI and the yields of milk fat, ECM, FCM, de novo FA, mixed FA, and preformed FA compared with diets without acetate. Diets containing cottonseed increased the yields of milk and preformed FA, tended to increase the yields of FCM and protein, and decreased DMI and the yields of de novo FA and mixed FA compared with diets without cottonseed. In summary, in high PA diets, the inclusion of acetate plus cottonseed increased milk fat yield compared with the other treatments. The CON diet in High PA increased milk fat yield to the same extent as AC and CS+AC in Low PA suggesting PA is important for initiating milk TG synthesis. Balancing the supply of de novo FA substrates and preformed FA is important for increasing the synthesis of milk fat triglycerides and milk fat production.

Growth, yield, and fiber quality of cotton plants under drought stress are positively affected by seed priming with potassium nitrate

Growth, productivity, and fiber quality of cotton (Gossypium hirsutum L.), a vital fiber-producing cash crop, are severely affected under drought conditions. Seed priming has a proven role in enhancing crop tolerance to abiotic stress, including drought. The objective of this study was to evaluate the effects of seed priming with KNO3 on cotton productivity and fiber quality under drought stress. A germination experiment was established under laboratory conditions with five treatments of seed priming (non-primed or control treatment, hydropriming, and priming with 2.5, 5.0, and 7.5 g KNO3 L−1). Another experiment under polyhouse conditions based on the same seed priming treatments was conducted under three levels of soil water contents (field capacity [FC] 100%: FC100, 75%: FC75, and 50%: FC50). The results obtained showed that there was a clear reduction in the different parameters tested at FC50 in comparison to FC100 (39–54%, 32–44%, 6–12%, and 7–12% reduction for boll number per plant, seed cotton yield, fiber strength, and leaf relative water content, respectively, across all priming treatments). Seed priming with KNO3 at 5 g L−1 effectively alleviated the detrimental effects originated from drought stress and caused 61–73%, 13–16%, and 16–23% increase in seed cotton yield, fiber length, and fiber strength, respectively, across soil moisture levels when compared with the control treatment. The same KNO3 dose caused an increase of 78% in water productivity in comparison to the control plants at FC50. Priming cotton seeds with 5 g KNO3 L−1 holds promise to obtain synchronized germination, enhance fiber quality, and increase yield, especially under water-limited conditions, thereby promoting economic viability and environmental sustainability. Additionally, this practice enhances water productivity, leading to significant water savings and reduced irrigation costs for cotton farmers. This method could be used as potential technology in advancing sustainable agriculture in water-constraint conditions.

Characterization of Caulimovirid-like Sequences from Upland Cotton (Gossypium hirsutum L.) Exhibiting Terminal Abortion in Georgia, USA.

In this study, we investigated the potential involvement of endogenous viral elements (EVEs) in the development of apical tissue necrosis, resulting in the terminal abortion of upland cotton (Gossypium hirsutum L.) in Georgia. The high-throughput sequence analysis of symptomatic and asymptomatic plant tissue samples revealed near-complete EVE-Georgia (EVE-GA) sequences closely related to caulimoviruses. The analysis of EVE-GA's putative open reading frames (ORFs) compared to cotton virus A and endogenous cotton pararetroviral elements (eCPRVE) revealed their similarity in putative ORFs 1-4. However, in the ORF 5 and ORF 6 encoding putative coat protein and reverse transcriptase, respectively, the sequences from EVE-GA have stop codons similar to eCPRVE sequences from Mississippi. In silico mining of the cotton genome database using EVE-GA as a query uncovered near-complete viral sequence insertions in the genomes of G. hirsutum species (~7 kb) but partial in G. tomentosum (~5.3 kb) and G. mustelinum (~5.1 kb) species. Furthermore, cotton EVEs' episomal forms and messenger RNA (mRNA) transcripts were detected in both symptomatic and asymptomatic plants collected from cotton fields. No significant yield difference was observed between symptomatic and asymptomatic plants of the two varieties evaluated in the experimental plot. Additionally, EVEs were also detected in cotton seeds and seedlings. This study emphasizes the need for future research on EVE sequences, their coding capacity, and any potential role in host immunity or pathogenicity.

Numerical Simulation and Bench Test of Crawler-Type Cotton Time-Delay Hole-Forming Device Based on RecurDyn-EDEM Coupling

In view of the challenges faced by cotton dibbler in Xinjiang under high-speed operation, a novel crawler-type delayed hole-forming device has been designed to address the seed throwing and floating issues in high-speed cotton dibbling in Xinjiang, enhancing the duck bill’s performance. This mechanism increases the sowing speed to 6 km/h by extending the duck bill horizontally. Utilizing agronomic principles, the mechanism’s layout and key components were optimized for efficient hole-forming. DEM and multi-body dynamics simulations were employed to analyze the motion, focusing on the fixed the tilt angle of the duck bill (A), the depth of the duck bill hole-forming into the soil (B), and the angle of rotation of the moving duck bill (C) as factors affecting hole dimensions (longitudinal length of hole Y1 and hole-forming depth of cotton seed hole Y2). Quadratic regression test using RecurDyn-EDEM coupling identified optimal parameter settings for maximum hole-forming performance. When A was 2.4°, B was 42.4 mm, and C was 30.5°, the performance of the hole-forming was the best. Under the optimal parameter combination, the bench verification test was carried out. The error between the bench verification results and the simulation results is small, indicating that the model has high accuracy. The average opening time of the duck bill at a speed of 6 km/h is 0.45 s, which is much longer than the time required for cotton seeds to fall from the duck bill (0.11 s). It meets the requirements of high-speed cotton planting in China and facilitating advancements high-speed planter technology.

Reduction of Emissions in a Single Cylinder Diesel Engine using Blends of Diesel &amp; Cotton Seed Oil with Unconventional Catalytic Converter

This paper investigates the feasibility of mitigating emissions from diesel engines through the combined application of alternative fuels and an unconventional catalytic converter employing cost-effective catalysts. The dwindling reserves of fossil fuels necessitate the exploration of sustainable power sources for internal combustion engines, thereby reducing our dependence on these finite resources. This study evaluated the performance parameters and emissions of a single-cylinder, four-stroke, stationary diesel engine fueled with blends of 10%, 20%, and 30% cottonseed oil in diesel (by volume). Following the identification of the optimal blend, a performance test was conducted again with the inclusion of a custom-designed and fabricated catalytic converter. Exhaust emissions were subsequently measured with and without the converter in operation. The design of the unconventional catalytic converter considered the engine's specifications, incorporating cerium oxide and sponge iron as oxidation catalysts for CO and hydrocarbon (HC) conversion. Charcoal was employed as a reduction catalyst to specifically target NOx emissions.

In vitro and In vivo Antioxidant Effect of Mango, Coconut and Cotton Seed Oils on Hydrogen Peroxide- Induced Oxidative Stress in Wistar Rats

This study is aimed at determining the antioxidant activity of Mango kernel oil (MKO), Coconut oil (CCO) and Cotton seed oil (CSO) in vitro and in vivo. Antioxidant parameters demonstrated by five spectrophotometric methods such as: DPPH, FRAP, MCA, HRSA and SRSA was determined in the oils. Superoxide dismutase activity (SOD), catalase activity (CAT) and malondialdehyde (MDA) were evaluated in the serum of the Wistar rats. The antioxidant assay of the oils showed Coconut oil (75.67±0.21 %) was able to neutralize the DPPH radical more than the other oils followed by Cotton seed oil (38.70±0.23 %) and Mango kernel oil (31.56±0.24 %). Coconut oil exhibited the highest FRAP activity at (1.04 mMolFe2+) while the Cotton seed oil displayed significantly lower (P&lt; 0.05) FRAP activity at (0.18±0.00 mMolFe2+). The Metal chelating activity (MCA) of Coconut oil (52.72±0.24 %) was found to be significantly higher than Mango and Cotton seed oils, Superoxide radical scavenging activity showed Coconut oil (62.36±0.01 %) having a relatively high ability to scavenge superoxide radicals better than the other oils with the standard glutathione being significantly higher (81.36±0.07 %). The result of the in vivo study showed the mean values for the SOD of MKO (118.10±5.39 IU/L), CCO (120.53±4.53 IU/L) and CSO (108.80±3.33 IU/L), CAT of MKO (108.93±11.60 IU/L), CCO (96.85±11.69 IU/L) and CSO (88.28±10.66 IU/L) and MDA of MKO (0.25±0.07 mmol/L), CCO (0.24±0.14 mmol/L) and CSO (0.31±0.02 mmol/L). The oils markedly reduced the amount of MDA while significantly increasing the activities of both CAT and SOD content.

Design and Test of a Crawler-Type On-Film Precision Cotton Seeding Device on DEM-CFD

To solve the limitation of speed in the operation of air-suction cotton precision seeding devices, a new crawler-type precision on-film seeding device was designed using an extended duckbill hole-forming device to produce hole-forming belts and lateral seed collection. Coupling simulation of seeding, and analysis of drag force and movement speed of cotton seeds were conducted on EDEM-CFD. A mechanical model of the operation was established, and the effects of the suction hole diameter, pressure relief block length, and wind pressure on seeding performance were analyzed. Regression models between influencing factors and seeding performance indices were established using response surface methodology. The test data were analyzed using Design Expert 10.0.3. The influence rules for each factor on seeding performance were established. The factors affecting the seeding qualified index exhibited the following order: air pressure &gt; pressure relief block length &gt; suction hole diameter. The optimization goals included defining the maximum value of a qualified single-seed index and the minimum value of the missing index, and the best parameter combination was obtained based on a suction hole diameter of 3.624 mm, pressure relief block length of 63.369 mm, and wind pressure of 3.797 kPa. According to the actual test conditions, the parameters were revised to include a suction hole diameter of 3.6 mm, a relief block length of 63.4 mm, and wind (air) pressure of 3.8 kPa for verification tests. The actual qualified single-seed index is 94.87% ± 0.98 %, the missing index is 1.45% ± 0.32%, and the multiple index is 3.68% ± 0.62%. The difference from the predicted value is within 5%, indicating that this study can provide a reference for the structural design and optimization of crawler-type on-film precision cotton seeding devices.