Costa Rica Research Articles

Changes in the economics of coffee production between 2008 and 2019: a tale of two Central American countries

Increasing costs of coffee production relative to coffee prices has led to concern across the industry of lack of profitability of coffee production especially for smallholders who comprise a large majority of producers. This study compares coffee production costs and income over a decadal interval of 2008 versus 2019 for coffee farmers in some of the main coffee growing regions of Costa Rica and Guatemala. Costs and income were collected by farmer recall using a standard questionnaire with trained research surveyors. Net income as assessed by EBITDA (earnings before interest, taxes, depreciation and amortization) increased by about 30% in Costa Rica, but declined to a third of its 2008 level in Guatemala. Agronomic costs of production per hectare increased by 31% in Costa Rica and 62% in Guatemala, mostly due to increased labor costs (higher daily wage rates), while fertilizer usage increased but unit costs remained stable. Gross income was stable in Guatemala but increased in Costa Rica due to receiving significantly higher prices for their coffee in 2019 compared to 2008, while in Guatemala prices declined. Nevertheless, the response was not uniform between farms in Costa Rica while high and medium productivity groupings of farms had higher EBITDA, low and very low productivity farms experienced a decline similar to Guatemala. The difference in performance of farm groups in Costa Rica was due to a decline in production per hectare of the lower productivity group; while the difference between Guatemala and Costa Rica was firstly due to price differences, and secondarily due to lower productivity of some farm groups. The investment of Costa Rican farmers was undoubtedly supported by the substantially increased price received by farmers (as compared to Guatemala), reflected in the increase in export price of coffee from Costa Rica relative to Guatemala. This shows the importance of farmers receiving higher prices for their produce in enabling them to cover increasing production costs, invest in increasing productivity and maintain profitability.

Bocconia frutescens little leaf, a new plant disease associated to a ´Candidatus Phytoplasma pruni´ related strain in Costa Rica

Objective/Background. Bocconia frutescens (Papaveraceae) is a small tree distributed naturally from Mexico to Argentina and the Caribbean Bassin. Bocconia trees showing symptoms resembling phytoplasmas infection, such as little leaves and witches´-broom, were found in Cartago province, Costa Rica. Detection and identification of the potential phytoplasmas associated with B. frutescens little leaf symptoms was the objective out of this study. Materials and Methods. Evaluation of leaves tissue using transmission electron microscopy (TEM), nested PCR using universal and specific primers to amplify phytoplasmas 16S rRNA and secA genes. Nucleotidic sequences (Sanger method) were obtained from amplicons, and used for BLASTn, phylogenetic analyses, and in silico RFLP’s. Results. Presence of phytoplasmas into phloem tissue, only in symptomatic trees, was evidenced by TEM. Comparison of partial sequences (16Sr and secA genes) by BLASTn, in silico RFLP´s and phylogenetic analyses, showed the occurrence of a ´Candidatus Phytoplasma pruni´ related strain in the samples evaluated. Conclusion. Phytoplasmas were found only in the symptomatic B. frutescens trees evaluated. The phytoplasmas were identified as a ´Ca. Phytoplasma pruni´ related strain. This is the first report of B. frutescens as a natural host of ´Ca. Phytoplasma pruni´.

Multinational evaluation of anthropometric age (AnthropoAge) as a measure of biological age in the USA, England, Mexico, Costa Rica, and China: a population-based longitudinal study.

To validate AnthropoAge, a new metric of biological age (BA), for prediction of all-cause mortality and age-related outcomes and characterize population-specific aging patterns using multinational longitudinal cohorts. We analyzed harmonized multinational data from the Gateway to Global Aging, including studies from the US, England, Mexico, Costa Rica, and China. We used body mass index and waist-to-height ratio to estimate AnthropoAge and AnthropoAgeAccel in participants aged 50-90 years old as proxies of BA and age acceleration, respectively. We compared the predictive capacity for all-cause mortality of AnthropoAge and chronological age (CA) using Cox models, described aging trends in all countries and explored the utility of longitudinal assessments of AnthropoAgeAccel to predict new-onset functional decline and age-related diseases using generalized estimating equations (GEE). Using data from 55,628 participants, we found AnthropoAge (c-statistic 0.772) outperformed CA (0.76) for prediction of mortality independently of comorbidities, sex, race/ethnicity, education, and lifestyle; this result was replicated in most countries individually except for Mexico. Individuals with accelerated aging had a ∼39% higher risk of death, and AnthropoAge also identified trends of faster biological aging per year. In longitudinal analyses, higher AnthropoAgeAccel values were independently predictive of self-reported health deterioration and new-onset deficits in basic/instrumental activities of daily living (ADL/IADL), diabetes, hypertension, cancer, chronic lung disease, myocardial infarction, and stroke. AnthropoAge is a robust and reproducible BA metric associated with age-related outcomes. Its implementation could facilitate modeling trends of biological aging acceleration in different populations, although recalibration may enhance its utility in underrepresented populations such as individuals from Latin America.

When the predator becomes the prey: new records of intraguild predation among Central American and Caribbean arachnids (Arachnida: Amblypygi, Araneae, Scorpiones)

ABSTRACT Intraguild predation occurs when an organism preys on a competitor. This ecological interaction can have considerable consequences on the population dynamics of predators and indirectly affect their prey. In arachnids, it is a quite common phenomenon, but it has been poorly documented in the Neotropical region. Here, we present records of 16 species feeding on other 14 species of arachnids. The most common families that acted as predators were Buthidae (Scorpiones) and Salticidae (Araneae), while the most common prey were members of the families Araneidae and Ctenidae (Araneae). In our survey, Drake Bay, in Costa Rica, is overrepresented due to years of systematic observations by two of the coauthors.

Regional Workshop on Optimization of Protection in Pediatric Interventional Radiology in Latin American and Caribbean Countries.

The purpose of this article is to describe the activities developed within the framework "Regional Workshop on Optimization of Protection in Pediatric Interventional Radiology in Latin American and Caribbean countries," developed between October 16th and 19th of the year 2023 in the city of San José, Costa Rica. The workshop was carried out as part of a joint work between the Pan American Health Organization (PAHO) and the World Health Organization (WHO), in cooperation with the International Atomic Energy Agency (IAEA). The main objective of the regional workshop was to gather the experiences and future work planning among participants in the Optimization of Protection in Pediatric Interventional Radiology in Latin America and the Caribbean (OPRIPALC) program. It involved professionals from 14 centers across 11 countries in the region, along with 4 experts from PAHO/WHO/IAEA. The work modalities during the workshop consisted of keynote presentations, individual presentations, group work, and general discussions. An online survey was carried out after the workshop, with the objective of knowing the opinion of the event participants and determining the impact and projection of the OPRIPALC program. During the workshop the centers had to present their experiences: the use of the DOLQA dose management system was presented and work was done on the consensus document on good practices. The activities, topics and organization of the workshop were valued positively by the participants. There is unanimity among the centers that the OPRIPALC program has had a positive impact and they wish to continue actively participating in the next biennium.

Description of Babesiacoryicola sp. nov. from Florida pumas (Puma concolor coryi) from southern Florida, USA

Previously, a high prevalence of piroplasms has been reported from Florida pumas (Puma concolor coryi) from southern Florida. In the current study, we describe the biological characteristics of a novel Babesia species in Florida pumas. Ring-stage trophozoites were morphologically similar to trophozoites of numerous small babesids of felids including B. leo, B. felis, and Cytauxzoon felis. Parasitemias in Florida pumas were very low (<1%) and hematologic values of 25 Babesia-infected Florida pumas were within normal ranges for P. concolor. Phylogenetic analysis of near full-length 18S rRNA gene, β-tubulin, cytochrome c oxidase subunit I, cytochrome c oxidase subunit III, and cytochrome b gene sequences indicated that this Babesia species is a member of the Babesia sensu stricto clade and is related to groups of Babesia spp. from carnivores or ungulates, although the closest group varied by gene target. Internal transcribed spacer (ITS)-1 region sequences from this Babesia sp. from 19 Florida pumas were 85.7–99.5% similar to each other and ∼88% similar to B. odocoilei. Similarly, an ITS-2 sequence from one puma was 96% similar to B. bigemina and 92% similar to a Babesia sp. from a red panda (Ailurus fulgens). Infected pumas were positive for antibodies that reacted with B. odocoilei, B. canis, and B. bovis antigens with titers of 1:256, 1:128, and 1:128, respectively. No serologic reactivity was noted for Theileria equi. No molecular evidence of congenital infection was detected in 24 kittens born to 11 Babesia-infected female pumas. Pumas from other populations in the United States [Louisiana (n = 1), North Dakota (n = 5) and Texas (n = 28)], British Columbia, Canada (n = 9), and Costa Rica (n = 2) were negative for this Babesia sp. Collectively, these data provide morphologic, serologic, genetic, and natural history data for this novel Babesia sp. which we propose the name Babesia coryicola sp. nov. sp. This is the first description of a felid-associated Babesia species in North America.

Geomorphology, morphodynamic, and surficial material of the Orosi Valley, Costa Rica

The Orosi Valley in Costa Rica holds significant geomorphological importance due to its diverse landscapes shaped by tectonic activity, river systems, erosion processes, and rich biodiversity. Moreover, this valley is important for agriculture, tourism, water reservoirs, and hydroelectricity production. This study presents a comprehensive geomorphological mapping of the Orosi Valley in Costa Rica. Six distinct geomorphological units have been identified and classified through a combination of field surveys, topographic data analysis, and remote sensing techniques. These units include hillslopes, alluvial fans, terraces at the valley margins, volcanic landforms, colluvial deposits, landslide scars, and fluvial landforms. The results provide valuable insights into the geomorphological processes and landform evolution within the Orosi Valley, contributing to scientific knowledge, land use planning, natural hazard assessment, and environmental management in the region. This study holds global importance by deepening the understanding of how geomorphology, disaster risk, and socio-economic development are interconnected. It informs worldwide initiatives to create resilient communities in response to evolving environmental hazards.

Shaping the future: Proposed bills to regulate new and emerging tobacco and nicotine products in Latin America and the Caribbean amidst public health concerns and industry interests.

To examine legislative efforts to regulate new and emerging tobacco and nicotine products (NETNPs) in Latin America and the Caribbean (LAC) between 2017 and 2022. Analysis of on-line national legislation databases to track NETNP bills using standard snowball search methods. Bills were identified in 15 out of the 33 LAC countries, accounting for a total of 91 bills targeting NETNPs (Table 1). Mexico had the highest number (n=38) of bills, followed by Brazil (n=16), Argentina (n=10), Colombia (n=8) and Chile (n=5). The remaining countries introduced two or fewer NETNP bills. Among the identified 91 bills, the majority (n=60) addressed both electronic cigarettes and HTPs, almost one third of bills (n=29) applied only to electronic cigarettes, and only two focused solely on HTPs. Six of the 15 countries with identified bills, enacted one NETNP law including Barbados, Bolivia, Costa Rica, Guyana, Mexico, and Panama. Since 2019, there has been a steady increase in the amount of NETNP bills introduced in LAC. In 2017 and 2018, 10 NETNP bills were introduced each year followed by 23 NETNP bills introduced in each 2019 and 2020, 19 in 2021, and 16 by August 2022. The substantial increase in the number of NETNP bills introduced since 2019 indicates the growing importance of regulating NETNPs in LAC. As NETNPs continue to evolve and grow it is important to continue monitoring and evaluating tobacco and nicotine regulatory initiatives while also staying ahead of the evolving market of NETNPs. Previous studies have analyzed the regulatory environment of new and emerging tobacco and nicotine products (NETNPs) but it is less known about legislative and regulatory efforts and attempts. This is the first known study to analyze legislative bills to regulate NETNPs in Latin America and the Caribbean (LAC). The substantial increase in the number of NETNP bills introduced in LAC since 2019 indicates the growing importance of regulating NETNPs in LAC. As NETNPs continue to evolve and grow it is important to continue monitoring and evaluating tobacco and nicotine regulatory initiatives while also staying ahead of the evolving market of NETNPs.

Incidence and recurrence of bovine abortion in dairy cattle from Costa Rica

The reproductive efficiency and milk yield of cows are crucial factors in a dairy farm's profitability. However, abortions can have a negative impact on these factors. While the morbidity of abortion has been estimated in many countries, information on the burden on dairy cattle in tropical conditions is limited, and Costa Rica is a good example. This study aims to assess the incidence and recurrence of bovine abortion in dairy cattle from Costa Rica. The study analysed the morbidity of abortion in Costa Rican dairy herds between 2010 and 2022. The incidence rate (IR) and the recurrence rate (ReR) were calculated per 100 cow-months at risk using data from the Veterinary Automated Management and Production Control Programme (VAMPP). The dataset comprised 1032,457 lactations from 330,265 cows in 1134 specialized dairy herds. Abortions were classified either as early foetal mortality (EFM) or late foetal mortality (LFM). Rates were estimated based on cow breed, lactation number, and ecological zone to which the farm belongs. The IR of general abortion, EFM, and LFM cases were 0.98, 0.41, and 0.57 per 100 cow-months at risk, respectively. No statistically significant differences were found in the IR between cow breed, lactation number, and ecological zone, nor for the trend of abortions over calving years. The first ReR (for cows that had one previous abortion during the lactation) was 0.95, and the second ReR (for cows that had two previous abortions during the lactation) was 1.41 per 100 cow-months at risk. These results suggest that bovine abortions are an important ongoing problem in dairy farms in Costa Rica with potentially detrimental effects on the reproductive and productive performance of cows and may be representative of other specialized tropical dairy systems in Latin America.

Traditional rural dietary pattern and all-cause mortality in a prospective cohort study of elderly Costa Ricans: the Costa Rican Longevity and Healthy Aging Study (CRELES)

BackgroundCosta Rica is experiencing a fast demographic aging. Healthy diets may help to ameliorate the burden of aging-related conditions. ObjectiveThis study aimed to investigate the association of a traditional dietary pattern and 2 of its major components (beans and rice) with all-cause mortality among elderly Costa Ricans. MethodsThe Costa Rican Longevity and Healthy Aging Study (CRELES), a prospective cohort study of 2827 elderly Costa Ricans (60+ y at baseline), started in 2004. We used a food frequency questionnaire (FFQ) to assess usual diet. We calculated dietary patterns using principal component analysis. Multivariate energy-adjusted proportional-hazards models were used to estimate hazard ratios (HRs) and 95% confidence intervals (CIs). ResultsOver a 15-y follow-up, encompassing 24,304 person-years, 1667 deaths occurred. The traditional Costa Rican dietary pattern was more frequent in rural parts of the country, and it was inversely associated with all-cause mortality. Subjects in the fifth quintile of intake had 18% lower all-cause mortality than those in the first quintile (HR: 0.82; 95% CI: 0.69, 0.98; P-trend = 0.01), particularly among males (HR: 0.73; 95% CI: 0.56, 0.95). Bean intake was associated with lower all-cause mortality among all subjects (HR: 0.79; 95% CI: 0.68, 0.91, highest compared with lowest tertile) and in sex-stratified analysis. Rice consumption was inversely associated with all-cause mortality solely among males (HR: 0.75; 95% CI: 0.60, 0.94, highest compared with lowest tertile). ConclusionsOur results suggest that a traditional Costa Rican rural dietary pattern is associated with lower all-cause mortality in elderly Costa Ricans. Beans, a major component of this traditional dietary pattern, was also associated with lower all-cause mortality. These findings could have important implications for public health, given the nutritional transition and the reduction of intake of traditional diets in Latin American countries.

Hematology and serum biochemistry of free-ranging mantled howler monkeys (Alouatta palliata) at La Pacifica, Costa Rica.

Hematologic and blood biochemical values are key tools for assessing primate health. A long-term behavioral study of howler monkeys at a single site (La Pacífica, Guanacaste, Costa Rica), afforded the opportunity to develop baseline values for a large group of animals, evaluating differences between adult males and females and comparing to a report in the same population two decades later. In 1998, 64 free-ranging mantled howler monkeys were anesthetized and sampled for hematologic and biochemical analysis. Blood analysis is reported for 29 adult females, 9 juvenile females, 19 adult males and 3 juvenile males. Four adults were excluded due to external injury or disease. There were few significant differences between adult females, juvenile females, and adult males. Baseline blood parameters are useful for determining normal values for howler monkey populations. The values for total protein, blood urea nitrogen, glucose, liver enzymes and potassium differed from a later study in 2019 may indicate changes that are influencing howler monkey health.

Alternatives for the Detection and Diagnosis of Osteoarticular Infections: An Exploratory Review.

The precise diagnosis of osteomyelitis, a bone infection, remains a significant challenge for healthcare professionals. This difficulty stems from the highly variable nature of its clinical presentation and disease course. Patients can exhibit a wide range of symptoms, making it easy to misdiagnose the condition. In turn, inaccurate diagnoses lead to inappropriate treatment regimens, potentially hindering a patient's recovery and causing unnecessary complications. Nuclear medicine offers a ray of hope in this fight against diagnostic ambiguity. It provides valuable tools, such as radiopharmaceutical imaging, that can significantly improve the accuracy of osteomyelitis diagnosis. However, limitations exist. This article explores the need for alternative diagnostic approaches within the specific context of Costa Rica. This exploration is particularly relevant due to the current regional shortage of gallium-67 (⁶⁷Ga), a radiopharmaceutical commonly used in osteomyelitis diagnosis. The article delves into the nature, function, and limitations of various nuclear medicine techniques, encompassing both independent radiopharmaceuticals like ⁶⁷Ga and those conjugated with specific targeting molecules to pinpoint areas of infection within the body. Given the scarcity of ⁶⁷Ga in Costa Rica, it becomes crucial to explore and implement viable alternative diagnostic techniques within the healthcare system. This article emphasizes the need for further investigation into these alternatives, with the goal of improving diagnostic accuracy and ensuring optimal patient care. By implementing these alternatives, healthcare professionals in Costa Rica can effectively combat the challenges posed by osteomyelitis and pave the way for better patient outcomes.

Social disparities in flood exposure and associations with the urban environment in 44,698 neighborhoods in 276 cities in eight Latin American countries.

Climate change is expected to greatly increase exposure to flooding, particularly in urban populations in low- and middle-income countries. We examined within-city social disparities in exposure to flooding in 276 Latin American cities and associated features of the neighborhood urban environment. We used a spatially granular dataset of historical flood events from 2000 to 2018 to describe neighborhood flooding within cities across eight Latin American countries (Argentina, Brazil, Chile, Colombia, Costa Rica, Guatemala, Mexico, and Panama). We estimated the percentage of the population living in flooded neighborhoods, described social disparities in flooding based on neighborhood educational attainment, and compared the magnitude of disparities across and within cities. We used multilevel models to examine how city- and neighborhood-level factors are related to neighborhood flooding. We examined 44,698 neighborhoods in 276 cities from eight countries with a total of 223 million residents and 117 distinct flood events from 2000-2018. One in four residents in neighborhoods in the lowest education quintile lived in neighborhoods with flooding, compared to one in 20 residents of the highest neighborhood education quintile. Greater neighborhood flooding was associated with lower neighborhood-level educational attainment and with neighborhoods that were coastal, less dense (population or intersection), further from the city center, greener, and had steeper slopes. There was no association between city-level educational attainment and flooding. There are large social disparities in neighborhood flooding within Latin American cities. Residents of areas with lower education attainment face substantially higher risks of flooding. Policymakers must prioritize flood adaptation and recovery efforts in neighborhoods with lower socioeconomic position.

Metáfora conceptual y embodiment en la noción «Costa Rica verde» en Esencial Costa Rica

A semantic analysis is presented of the advertising language used by the brand Essential Costa Rica. A measurable strategy based on the conceptual metaphor “green is gold” is evident in these texts. It is examined using two macrocategories present in the words and expressions that make up the brand’s tourism advertising: “Costa Rica awaits a treasure” and “Costa Rica is a paradise.” They both generate an identity image of the region, apparently idyllic and paradisiacal, where socio-environmental problems typical of the reality that the country faces are distorted and become invisible.

A Comparative Approach for Pronunciation Instruction in English

A tool is proposed to reinforce the pronunciation of vowels and consonants for the instruction of adult learners in Costa Rica. A critical review of literature on approaches to teaching vowels and consonants is followed by a comparative discussion of two transcription systems: that of the International Phonetic Association, and those found in textbooks, dictionaries and pronunciation websites. This is complemented by a brief comparison of English and Spanish vowels and consonants, and descriptions of common pronunciation challenges for Spanish speakers. Open problems are explored, as are ethical reflections on the teaching of English pronunciation.

A route towards modern, creative and innovative services as a contribution to sustainable development: The case of Tourism in Costa Rica

Costa Rica, due to the characteristics of its territory, can offer modern, creative and innovative services in its tourism, based on knowledge, that use high technology and/or have qualified labor. Data from the BCCR indicates that, in the last decade, the percentage of tourism contribution to GDP was approximately 5%, therefore, a route is presented for sustainable and inclusive development, which enables quality jobs and promotes better redistribution of wealth among the inhabitants, framed with the commitment to the SDGs, adequate control of the macroeconomic variables of the domestic and international market; and changing the general tourism law, with the aim to have an activity governing body oriented to better coordination and management and its contribution to development.

“An intruder body”. Antidiscrimination law and the justiciability of the economic, social and cultural rights in the Inter-American Court of Human Rights: an analysis based on the Case of Guevara Díaz vs. Costa Rica

This article aims to analyze the Case of Guevara Díaz vs. Costa Rica, judged in June 2022 by the Inter-American Court of Human Rights, especially about Anti-Discrimination Law. The precedent innovates by establishing the normative force of the principle of progressive development provided for in article 26 of the American Convention on Human Rights (ACHR), understood, at least until then, as a mere interpretative principle of the ACHR. The decision also deserves to be highlighted due to the adoption of Anti-Discrimination Law as a backdrop to enable the direct justiciability of Economic, Social and Cultural Rights (DESCA). In terms of methodology, the research is carried out based on a critical-reflexive review of the topics covered and the use of the hermeneutic phenomenology method.

An updated checklist of Endomychidae and Anamorphidae (Coleoptera: Coccinelloidea) from El Salvador, with additional new records from the Neotropical region

The checklist of the species of fungus beetle families Endomychidae and Anamorphidae from El Salvador is updated. Only six species of Endomychidae and one Anamorphidae have been previously recorded from the country, with specific localities reported only for two of them. The study of specimens collected in El Salvador over 60 years ago by Jan and Bohumila Bechyné and held in the Royal Belgian Institute of Natural Sciences, Brussels, allowed us to have a better idea on the diversity and distribution of these beetles in the country. Eight species of Endomychidae and one of Anamorphidae arerep orted here, including three first records for El Salvador: Anidrytus guatemalae Arrow, Epipocus bifidus Gerstaecker andStenotarsus ovalis Arrow. Additionally, first records for species of Endomychidae from countries of the Neotropical region are provided: Anidrytus batesi Strohecker from Peru, A. ovatulus Gerstaecker from Guyana, Ephebus hirtulus Stroheckerfrom Paraguay, Epopterus vacuus Gerstaecker from Costa Rica and Acinaces unicolor Tomaszewska from Peru.

Mycena citricolor isolates from diverse hosts in Costa Rica: Colony aspect, growth dynamics, reproduction in vitro and aggressiveness on Coffea arabica

AbstractThe basidiomycete fungus Mycena citricolor hosts on about 80 plant families. The asexual morph causes the disease known as American leaf spot of coffee (Coffea arabica). We isolated and characterized 15 isolates of M. citricolor collected from coffee plants, shade trees and weeds. Cultures on potato‐dextrose‐agar + yeast extract (PDAY) medium were kept for a week in complete darkness. The cultures were then exposed to light for 7 weeks and co‐cultivated with coffee foliar discs, to stimulate the production of gemmifers. We evaluated the speed of growth, appearance, colour of the mycelium and gemmifers production capacity. All isolates conformed to the typical cultural characteristics of M. citricolor, with beige/white thin and slightly aerial mycelium. The whitest isolate was from Persea americana, which had the slowest growth rate in vitro (6.4 mm/day), while the isolate from weed Kalanchoe pinnata was the fastest, with a speed of 9 mm/day. The most gemmifer‐producing isolates were those isolated from the coffee cultivar Catimor‐Costa Rica 95, Citharexylum donnell‐smithii and Cissus verticillata, with over 200 gemmifers per Petri dish. Isolates from Erythrina and Ipomoea nil produced &lt;50 gemmifers on average. The Composite Aggressiveness Index (CAI) was determined on 2‐year‐old detached coffee branches of the Caturra variety foliage. The least aggressive isolate was from Erythrina poeppigiana (CAI = 0) and the most aggressive was from Kalanchoe pinnata (CAI = 18.87). The isolate from the coffee cultivar Catimor‐Costa Rica 95 was more aggressive than that from the cultivar Caturra (CAI = 9.94 vs. CAI = 3.66). Moreover, successful infection occurred only when the apex of gemmae made direct contact with the coffee leaf when using the inoculum derived from C. verticillata and Anredera cordifolia. These results show the variability in the population of the pathogen. This is the first study that characterizes the growth, morphology, reproduction and aggressiveness of M. citricolor obtained from hosts other than coffee. In addition, it is the first to report the use of coffee foliar discs to induce gemmifer production in vitro and to assess and quantify the effect of gemma position when inoculating coffee leaves.

Daily Intake of Carbohydrates and Added Sugars in Youngsters in Costa Rica

Daily Intake of Carbohydrates and Added Sugars in Youngsters in Costa Rica