Correlation Of Uric Acid Levels Research Articles

Correlation of serum uric acid levels with certain anthropometric parameters in prediabetic and drug-naive diabetic subjects.

Uric acid is produced during the metabolism of nucleotide and adenosine triphosphate and contains the final product of human purine metabolism. It acts both as an antioxidant and pro-inflammatory marker and has a positive association with visceral fat in overweight subjects. The aim of the present study is to find an association of uric acid level with certain anthropometric parameters in subjects having type 2 diabetes. The study included 124 urban drug-naive diabetic Indian subjects above 18 years of age from the general population of the city of North India. Uric acid concentrations were estimated by the uricase method. Fasting plasma glucose (FPG) concentrations were estimated by the glucose oxidase-peroxidase method. Anthropometric measurements and information on lifestyle factors and disease history were collected through in-person meeting. All participants of the study subjects had a body mass index (BMI) of more than 23.5. BMI, waist-to-hip ratio (WHR), waist-to-height ratio, waist circumference, neck circumference, weight, age, sagittal abdominal diameter (SAD), skinfold thickness, and body roundness index were positively correlated with the serum uric acid level. The correlation of weight, BMI, SAD, and WHR was statistically significant. We found that serum uric acid level increases as body fat content increases. Statistical data show remarkable results for a significant correlation of uric acid level with BMI, WHR, SAD, and FPG. Hypertrophy occurs as a result of inflammatory processes and oxidative stress when the supply of energy starts to exceed the storage capacity of adipocytes, as a result, adipokines such as interleukin (IL)-1, IL-6, and tumor-necrosis factor-alpha are released more frequently which lead to low-grade chronic inflammation. Uric acid levels are much lean toward visceral obesity than overall body fat content.

Correlation of Uric Acid Levels with Feto-Maternal Outcomes in Hypertensive Disorders in Pregnancy

Objective: To explore the correlation between uric acid levels and feto-maternal outcomes in women with hypertensive disorders of pregnancy.
 Study Design: Comparative cross-sectional study.
 Place and Duration of Study: Obstetrics & Gynaecology Department, Combined Military Hospital, Rawalpindi Pakistan, from Feb to Aug 2021.
 Methodology: In this cross-sectional study, 90 pregnant women with hypertensive disorders having greater than 26 weeks of gestation were included after seeking Ethical Committee approval. Selected parameters were noted on a structured proforma.
 Results: Among the participants, 38(42.2%) had pregnancy-induced hypertension, 32(35.5%) had pre-eclampsia, 13(14.5%) had chronic hypertension with pre-eclampsia, and 7(7.8%) had eclampsia. Mean Uric acid levels ranged between 363.66±50.45 μmol/L and 451.86±120.62μmol/L, with a significant difference between PIH and eclampsia (p<0.001). Mode of delivery was avaginal, primary cesarean section, and repeat cesarean section in 31(34.4%), 38(42.2%), and 21(23.4%) patients, respectively(p<0.001). Liquor was meconium stained in 49(54.4%) while clear in 41(45.6%) births, with (p<0.001). Early neonatal deaths6(6.7%) participants had significantly higher uric acid levels than no NICU admission 22(24.4%). In maternal outcomes, 83 patients (92.2%) required routine post-operative care, while 7(7.8 %) went to the intensive care unit. Uric acid levels had a negative correlation with gestational age.
 Conclusion: Maternal uric acid levels differ significantly in different hypertensive disorders of pregnancy and affect the mode of delivery and neonatal outcomes.

PS-C10-5: CORRELATION OF SERUM URIC ACID LEVELS WITH CERTAIN ANTHROPOMETRIC PARAMETERS AND ARTERIAL BLOOD PRESSURE IN OVER WEIGHT AND OBESE SUBJECTS

Background: Asymptomatic hyperuricemia without comorbidities predicts the development of hypertension. Moreover, hyperuricemia also contributes to the development of hypertension from prehypertension. Aim: To find out correlation of serum uric acid levels with certain anthropometric parameters and arterial blood pressure in over weight and obese subjects Methodolgy: The study included 224 urban drug naive over weight and obese Indian subjects above 18 years of age from general population of city of north India. Uric acid concentrations were estimated by the uricase method. Blood pressure was measured by using Automatic Digital Sphygnomanometer with mean of 3 readings following all standard pre requisite. Anthropometric measurements and information on lifestyle factors and disease history were collected by inperson meeting. Results: All participants of the study subjects had BMI more than 23.5. Correlation of weight, BMI, SAD, WHR, SBP & DBP was positively correlated & statistically significant. Pearsons Correlation between Anthropometric parameter and Serum Uric Acid Conclusion: Statistical data shows remarkable results for significant correlation of uric acid level with BMI, WHR, SAD, SBP & DBP. Hypertrophy results into and oxidative stress when the supply of energy starts to exceed the storage capacity of adipocytes, as a result adipokines such as interleukin-1, interleukin-6 and tumor-necrosis factor alpha are released more frequently which lead to low-grade chronic inflammation. Evidence indicates that uric acid can cause direct endothelial injury and dysfunction. Uric acid levels are much lean towards visceral obesity than overall body fat content.

The correlation of uric acid levels with biochemical parameters and dietary factors in individuals with asymptomatic hyperuricemia and gouty arthritis

Diet may play an important role in the development of asymptomatic hyperuricemia (ASH) and gouty arthritis (GOUT). However, the association between dietary factors and hyperuricemia remains unclear. Serum uric acid levels are affected by dietary factors. This study aimed to evaluate the correlation of uric acid levels with biochemical parameters and dietary factors in individuals with ASH and GOUT. This study was conducted in 145 individuals with ASH and GOUT. General characteristics of individuals were collected via face-to-face interviews. Food frequency questionnaire was used to obtain energy, macro- and micronutrients intakes. Biochemical parameters were obtained from patient files. The incidence of gout was higher in men comparing to women. Individuals in the GOUT group consumed more alcohol and higher serum levels of vitamin B12, C-reactive protein (CRP), triglyceride, and uric acid. Individuals in the GOUT group had higher intakes of energy, protein, carbohydrate, fat, fructose, vitamin C, and vitamin B12. Triglyceride, uric acid, CRP, vitamin B12, and homeostatic model assessment of insulin resistance were found to be affected by high uric acid levels. Dietary factors can pose a risk for health problems in addition to GOUT and ASH, such as cardiovascular disease, diabetes, and obesity.

Correlation of Uric Acid Levels and Rheumatoid Factor in the Elderly

Elderly people have dysfunctional changes in the immune system. Disease burden will increase due to uric acid particularly in elderly including arthritis. Rheumatoid factor (RF) is an examination that can identify the presence of rheumatoid arthritis. Reactive RF value in RA is 70%, while in people 65 years old is 20%. This research is to define the correlation between uric acid levels and rheumatoid factor in elderly. Methods use cross-sectional study; Participants: twenty-one elderly people. Measurements: whole blood and serum from elderly individuals, Accu Check, Antisera RF, Slide test. The research showed that normal uric acid levels with non-reactive RF were 15 people (93.8%) while with reactive RF was 1 person (6.3%). The results of uric acid levels increased with non-reactive RF were 2 people (40%) while with reactive RF was 3 people (60%). Uric acid levels and rheumatoid factor (RF) were analyzed using the Chi-square test. Both are interrelated (p=0,028, P< 0.05). The conclusion of this research is that uric acid levels are correlated with rheumatoid factor in the elderly.

Effects of Stress and Stress Hormones on Heart Rate Variability Parameters in 1st Year Medical Students

Stress in present world is inevitable where each individual in pursuit of achieving the maximum undergoes through this event. stress in medical education has resulted in decreasing the confidence of students and cause of various abnormal psychological conditions. Anxiety induced stress is common and this exaggerates during the time of examinations. This altered state of mind results in variation of heart rate variability indices and biochemical parameters which needs to be examined. The present study attempts to correlate stress with biochemical and HRV parameters. The present study inducted first year medical graduate course students after obtaining informed consent. 30 students participated in the study among them 18 were male and 12 female students with mean age of 17.9±0.55 years The students were subjected for 5minute recording of ECG under standard conditions for heart rate variability parameters. The recording of these parameters was done once when the students joined the course and the second recording was obtained after 6 months when the 2ndInternal examination Viva-voce were conducted similarly for evaluation of the biochemical physiological stressors was evaluated by collecting 5ml of venous blood and subjected to serum cortisol and uric acid levels analysis immediately using Fully automated Bidirectionally Interfaced Chemi Luminescent Immuno Assay for cortisol and Photometry for uric acid levels twice when the recording of heart rate variability (HRV) was done. Results were subjected to statistical analysis using statistical software sPss 20.0. students ”t” test and Pearson's tests were done. A p value of <0.05 was considered to be statistically significant. Our results showed correlation of uric acid levels with time domain parameters of heart rate variability and LF/HF ratio of frequency domain. The cortisol levels could not directly link with heart rate variability parameters. Serum cortisol and uric acid levels are good indicators for determination of stress as demonstrated in our study of academic environment. Though we could establish uric acid levels correlating with decrease in short term recording of heart rate variability parameters. We would suggest further studies with larger sample size and recordings during university examinations which would predict its usefulness. We suggest academic stress can lead to chronic illness and thus detoriate the quality of life lead by health professionals. Hence we suggest early intervention and change curriculum as well as evaluation process which can reduce the stress perceived by students.

Correlation of uric acid levels and parameters of metabolic syndrome.

Hyperuricemia has been described as associated with the risk of development metabolic syndrome; however the relationship between the uric acid level and particular parameters of metabolic syndrome remained unclear. We performed a cross-sectional study on a cohort of 833 dyslipidemic patients and correlated their levels of uric acid with parameters of insulin resistance, lipid metabolism, C-reactive protein, anthropometric parameters. We also defined patients with hypertriglyceridemic waist phenotype and compered their uric acid levels with those without this phenotype. We found that levels of uric acid are associated with parameters of metabolic syndrome. Specifically, dyslipidemia characteristic for metabolic syndrome (low HDL-cholesterol and high triglycerides) correlates better with uric acid levels than parameters of insulin resistance. Also waist circumference correlates better with uric acid levels than body mass index. Patients with hypertriglyceridemic waist phenotype had higher levels of uric acid when compared with patients without this phenotype. Serum uric acid levels are even in low levels linearly correlated with parameters of metabolic syndrome (better with typical lipid characteristics than with parameters of insulin resistance) and could be associated with higher cardiovascular risk.

Optimization of pH values to formulate the bireagent kit for serum uric acid assay

A new formulation of the bireagent kit for serum uric acid assay was developed based on the effects of pH on enzyme stability. At 4°C, half-lives of uricases from Bacillus fastidious and Arthrobacter globiforms were longer than 15 months at pH 9.2, but became shorter at pH below 8.0; half-lives of ascorbate oxidase and peroxidase were comparable at pH 6.5 and 7.0, but became much shorter at pH higher than 7.4. In the new formulation of the bireagent kit, Reagent A contained peroxidase, 4-aminoantipyrine, and ascorbate oxidase in 50mM phosphate buffer at pH 6.5; Reagent B contained B. fastidious or A. globiforms uricase in 50mM sodium borate buffer at pH 9.2; Reagents A and B were mixed at 4:1 to produce a final pH from 7.2 to 7.6 for developing a stable color. The new bireagent kit consumed smaller quantities of three enzymes for the same shelf life. With the new bireagent kit, there were linear responses of absorbance at 546nm to uric acid up to 34mM in reaction mixtures and a good correlation of uric acid levels in clinical sera with those by a commercial kit, but stronger resistance to ascorbate. Therefore, the new formulation was advantageous.