Corniculatus Var Research Articles

Distribution Strategies of Vascular Plants at the Reclaimed Lands at the Saemangum Sea Dike

The distribution of plant communities in the reclaimed land of the southwestern coasts of South Korea, along with the environmental or plant factors, was studied through canonical correspondence analysis (CCA) and the competitor (C), stress tolerator (S), and ruderal (R) (CSR) ecological strategies. The coastal reclaimed land plants were classified into three plant-factor groups in the CCA biplot diagram. Axis1 was correlated with LS, SLA, CH, and FS. Axis 2 was correlated with LDMC, FP, and LDW. The reclaimed landplants were classified into three soil-factor groups in the CCA biplot diagram. First, the group factor was correlated with SAND. Second, the group factor was distributed according to T-N and TOC. Third, the group factor was distributed according to Salinity content. To clarify the relative significance of competition, stress, and disturbance in the distribution process of plant communities, the CSR distribution model was adopted. Sixteen of the 19 species were allocated to the factors related to the ruderals strategies such as R/CR and CR including R/CSR, SR/CSR, and CR/CSR, and SC strategies. They displayed ruderal and competition adaptation strategies reflecting the ecological environment. Seven species showed R/CR (ruderal/competitor-ruderal) strategies; Aeschno meneindica, Chenopodium album var. centrorubrum, Lolium perenne, Lotus corniculatus var. japonicus, Phragmites communis, Portulaca oleracea, and Soncous brachyotus and 6 species showed CR (competitor-ruderal) strategies; Bidens frondosa, Echinochloa crus-galli, Echino chloaoryzicola, Erigeron canadensis, Fimbristy lislongispica and Setaria viridis. The three species with R-related strategies were Artemisia princeps, Lolium perenne, and Trifolium repens. The distribution patterns of the CCA diagrams and CSR triangles may provide a useful scientific basis for protecting and restoring reclaimed lands and their valuable ecosystem services, from the increasing disturbances.

Effects of Corrugated Cardboard-based Media on the Physicochemical Properties and Coverage of Plant

The physical and chemical properties of corrugated cardboard-based media were evaluated to assess their suitability as substrates for plants. Samples were collected periodically from the same study area over 5 months to determine any seasonal variabilities. In addition to the corrugated cardboard-based media, the rural soil of the study area was used as the control. The corrugated cardboard-based media showed adequate levels of moisture content and bulk density and correspondingly high porosity values when compared with the rural soil. All corrugated cardboard-based media showed adequate levels of electrical conductivity, organic matter, and correspondingly high cation exchange capacity values when compared with the rural soil in all seasons. The C/N ratio of the corrugated cardboard-based media was similar to the optimal values of 15-20. However, the pH values of the corrugated cardboard-based media were lower than the optimum value(pH 4) for plant growth and rural soil. The moisture content value which cycle bobs up and down according to sampling date, and this means that the moisture content is affected by seasonal changes in the components entering the study area. The corrugated cardboard-based media appear to be acceptable for growing plants(Lotus corniculatus var. japonicus). Finally, the use of this media permits better vegetation establishment than the use of rural soil. Our results prove that corrugated cardboard-based media can exhibit higher plant cover over a long period(September-November) as opposed to rural soil. Our study demonstrates the effectiveness of corrugated cardboard-based media for plant growth, and we recommend that future studies should focus on potential materials relevant to revegetation and management.

Allelopathic Effect of Dodder on Different Varieties of Lucerne and Bird’s Foot-Trefoil

Summary The aim of this study was to evaluate the allelopathic effect of cold aqueous extracts, both fresh and dry biomass of dodder (Cuscuta epithymum L.) on lucerne (Medicago sativa L.) and bird’s foot-trefoil (Lotus corniculatus L.). Four different varieties of lucerne “Pleven 6”, “Dara”, “Roly”, “Multifoliolate”) and bird’s foot-trefoil (“Gran San Gabriele”, “Leo”, “Local population 1”, “Local population 2”) were studied in order to find some varieties with allelopathic tolerance. Ex-situ experiment was carried out as follows: 100 seeds of each variety were put in Petri dishes between filter paper, both cold extracts of parasitic weed biomass were pipetted at a ratio of 1:20 as against the seed mass and then were placed in a thermostat-operated device at a temperature of 22 ± 2°С. Distilled water was used as a control. Percentage inhibition, Index of tolerance and Index of plant development were calculated for assessment of the allelopathic effect of dodder on the early seedling growth, biomass synthesis and initial development of experimental varieties. As a whole, dry weed biomass was found as more toxic for the tested plants than the fresh one. Medicago sativa var. multifoliolate and Lotus corniculatus var. Local population 1 and Local population 2 showed a significant tolerance to the allelopathic influence of Cuscuta epithymum in all studied concentrations of aqueous extract of fresh weed biomass (25, 50 and 100 g l−1) and medium tolerance to aqueous extract of dry weed biomass (concentrations of 25 and 50 g l−1).

훼손비탈면 복원을 위한 콩과목본류로서 참싸리 및 낭아초의 적정파종량에 관한 연구

The purpose of the research is to identify the optimal seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria as leguminous woody plants for the cut-slope revegetation. To investigate the coverage ratio and appearance frequency, we divided Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria into five treatment groups with various quantities of , , , , and . For each treatment group, we mixed the identical quantities of herbaceous flowers (Lotus corniculatus var. japonicus, Dianthus sinensis, Aster yomena and Pennisetum alopecuroides) seeds and cool-season turfgrasses (Festuca arundinacea and Poa pratensis) seeds. In this result, as the seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria increased in the spray, the coverage ratio for leguminous woody plants appeared to increase whereas the coverage ratio decreased in herbaceous flowers and cool-season turfgrasses. However, when the seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria exceeded in the spray, the coverage ratio of leguminous woody plants rather decreased compared to four treatment groups less than seeding ratio. Based on the longitudinal data of coverage ratio in five treatment groups, we observed the gradual process of a short-term succession in which the dominant species shifted in the following order: First, cool-season turfgrasses; Second, herbaceous flowers; Third, leguminous woody plants. Comparing the appearance frequency of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria, Lespedeza cyrtobotrya appeared more frequent in 2014 whereas Indigofera pseudo-tinctoria appeared to be relatively more frequent in 2015. As a result, Indigofera pseudo-tinctoria was discovered to be a dominant species among woody plants. In this study, we observed that the optimal seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria was . Moreover, the coverage ratio of 29.1%~35.4% and appearance frequency of 4.6plants~5.8plants were found in the condition of optimal seeding quantity.

몇몇 지피식물의 비탈면녹화공사 활용성 연구 - 억새, 톨훼스큐, 수크령, 한국잔디 -

Research was initiated to investigate a vegetation characteristics of the species of ground cover plants mixed with herbeceous and woody plants on a cut slope. 4 different ground cover plants (Miscanthus sinensis var. purpurascens, Pennisetum alopecuroides, Festuca arundinacea and Zoysia japonica) were seeded at the same rate of with herbaceous plants (Lotus corniculatus var. japonicus, Dianthus sinensis, Aster yomena, Chrysanthemum zawadskii and Coreopsis drummondii L) at the total seeding rate of and native woody plants (Lespedeza bicolor Turcz, Indigofera pseudo-tinctoria and Albizzia julibrissin) at the total seeding rate of , respectively. This experiment was treated with 3 replications on a randomized block design. Data such as surface coverage rate(%), the germinating number, plant height and visual quality were analyzed. There were no statistic differences observed in the soil hardness and the soil moisture content while a significant difference was observed in the surface coverage rate and the germinating number. The surface coverage rate and the germinating number were significantly high in the Pennisetum alopecuroides plot when compared with the Miscanthus sinensis var. purpurascens plot, the Festuca arundinacea plot and the Zoysia japonica plot, respectively from the viewpoint of ground covers. Especially, the surface coverage rate was considerably low in the Festuca arundinacea plot. However, the surface coverage rate and the germinating number were no statistic differences observed in this treatment from the viewpoint of herbaceous and woody plants. Also, both of the plant height and visual quality were high in the Pennisetum alopecuroides plot when compared with the Miscanthus sinensis var. purpurascens plot, the Festuca arundinacea plot and the Zoysia japonica plot, respectively. We concluded that Pennisetum alopecuroides was the optimum plant from the treated ground covers mixed with native woody and herbaceous plants for the balanced emergence from the viewpoint of the ecological slope vegetation.

Genome-wide analysis of AP2/ERF family genes from Lotus corniculatus shows LcERF054 enhances salt tolerance.

Lotus corniculatus is used in agriculture as a main forage plant. Members of the Apetala2/ethylene response factor (AP2/ERF) family play important roles in regulating gene expression in response to many forms of stress, including drought and salt. Here, starting from database of the L. corniculatus var. japonicus genome, we identified 127 AP2/ERF genes by insilico cloning method. The phylogeny, gene structures, and putative conserved motifs in L. corniculatus var. japonicus ERF proteins were analyzed. Based on the number of AP2/ERF domains and the function of the genes, 127 AP2/ERF genes from L. corniculatus var. japonicus were classified into five subfamilies named the AP2, dehydration-responsive element binding factor (DREB), ERF, RAV, and a soloist. Outside the AP2/ERF domain, many L. corniculatus var. japonicus-specific conserved motifs were detected. Expression profile analysis of AP2/ERF genes by quantitative real-time PCR revealed that 19 LcERF genes, including LcERF054 (KJ004728), were significantly induced by salt stress. The results showed that the LcERF054 gene encodes a nuclear transcription activator. Overexpression of LcERF054 in Arabidopsis enhanced the tolerances to salt stress, showed higher germination ratio of seeds, and had elevated levels of relative moisture contents, soluble sugars, proline, and lower levels of malondialdehyde under stress conditions compared to wild-type plants. The expression of hyperosmotic salinity response genes COR15A, LEA4-5, P5CS1, and RD29A was found to be elevated in the LcERF054-overexpressing Arabidopsis plants compared to wild type. These results revealed that the LcERF genes play important roles in L. corniculatus cv Leo under salt stress and that LcERFs are attractive engineering targets in applied efforts to improve abiotic stress tolerances in L. corniculatus cv Leo or other crops.

Divergence of Flowering‐Related Genes in Three Legume Species

We used a set of approximately 200 Arabidopsis thaliana (L.) Heynh. genes that are involved in the control of flowering time as a reference to identify orthologous (or homologous) counterparts of these genes in three legume species, that is, Lotus corniculatus L. var. japonicus Regel [syn. Lotus japonicus (Regel) K. Larsen], Medicago truncatula Gaertn. (barrel medic), and soybean [Glycine max (L.) Merr.]. A total of 96, 98, and 304 homologs of flowering genes were identified in L. corniculatus var. japonicus, M. truncatula, and G. max, respectively. Most of these genes were categorized into seven different flowering pathways, including photoperiod, vernalization, gibberellins, autonomous pathways, floral pathway integrators (FPIs), and floral meristem identity. Many key genes, including the FPI genes FT, SOC1, and LFY, are conserved in the legumes while CO, FRI, FLC, and FD were not. Eighteen genes were conserved as single copy genes in all three legume species, including GI, VRN2, COP1, and TSF. The chromosomal distribution of paralog‐rich genes revealed differences in the major evolutionary processes affecting flowering genes in legumes, including whole genome duplication in soybean, tandem duplication in M. truncatula, and ectopic duplication in L. corniculatus var. japonicus. High divergence was observed among the members of large gene families, most containing transcription factors, indicating the accumulation of gene copies and gene divergence during evolutionary adaptations to environmental changes.

Deep sequencing of Lotus corniculatus L. reveals key enzymes and potential transcription factors related to the flavonoid biosynthesis pathway

Lotus corniculatus L. is used worldwide as a forage crop due to its abundance of secondary metabolites and its ability to grow in severe environments. Although the entire genome of L. corniculatus var. japonicus R. is being sequenced, the differences in morphology and production of secondary metabolites between these two related species have led us to investigate this variability at the genetic level, in particular the differences in flavonoid biosynthesis. Our goal is to use the resulting information to develop more valuable forage crops and medicinal materials. Here, we conducted Illumina/Solexa sequencing to profile the transcriptome of L. corniculatus. We produced 26,492,952 short reads that corresponded to 2.38 gigabytes of total nucleotides. These reads were then assembled into 45,698 unigenes, of which a large number associated with secondary metabolism were annotated. In addition, we identified 2,998 unigenes based on homology with L. japonicus transcription factors (TFs) and grouped them into 55 families. Meanwhile, a comparison of four tag-based digital gene expression libraries, built from the flowers, pods, leaves, and roots, revealed distinct patterns of spatial expression of candidate unigenes in flavonoid biosynthesis. Based on these results, we identified many key enzymes from L. corniculatus which were different from reference genes of L. japonicus, and five TFs that are potential enhancers in flavonoid biosynthesis. Our results provide initial genetics resources that will be valuable in efforts to manipulate the flavonoid metabolic pathway in plants.

준설토 파종식물의 생육경향 및 관리방안 연구 -낙동강 14공구 중심으로-

This study was launched to verify the effective composition of plant species and its management program most suited for the dredged soiled area near Nakdong River Site 14. The improvement methods of planting base and the composition of plants such as silver grass, reed, and some colonies in aesthetic effect were studied. To search the management methods to decrease the confining pressure risen from the burried seeds which would consequently harm the previously seeded plants, experimental construction process was measured on the site. The purpose of this experiment was to figure out which part of the improvement on the plant base has the most significant effect for the revegetation of infertile, dredged soil, to verify the easily seeded, developing plants among seeded plants, and finally, to find the restoration model using plants near the dredged soil around riverside. 8 seeded plants and 23 invaded species were appeared which among the emerged plants, development of Aster yomena MAKINO, Lotus corniculatus var. japonica Regel, Trifolium repens L, and Dianthus longicalyx Miq were proved to be brought up well. Difference risen from the seed composition were not noticeable until 150day since the germination was proceeded mainly by Aster yomena MAKINO. The experimental plot with dredging sand+organic fertilizer method of construction and dredging sand+soil conditioner method showed most development while the effect of the plot with only the soil base of dredging sand stayed low. Another important method for the management of infertile, dredged soil base would be the removal of disturbing species which the experiment showed the tied relationship between the removal of disturbing species and development of seeding plants. Although this study was carried out focused on the Nakdong River Project, the study suggests the general management program that the removal of disturbing species such as Humulus japonicus Sieboid & Zucc. and Pueraria lobata (Willd.) Ohwi in times around rainy season(60days after seedling) would be effective for the easy growth of revegetation plants.

Comparative Antitumor Activity of Jelly Ear Culinary-Medicinal Mushroom, Auricularia auricula-judae (Bull.) J. Schrot. (Higher Basidiomycetes) Extracts Against Tumor Cells In Vitro

The present study compares the antitumor activity of extracts from Auricularia auricula-judae, Phellinus gilvus, Ganoderma lucidum, and 100 Korean wild plants in the P388D1 macrophage cell line. The antitumor activity of A. auricula-judae extract (44.21%) did not differ significantly (P < 0.05) from those of Ph. Gilvus (39.46%) and G. lucidum (36.64%) at 1 mg/mL of concentration. Among 100 wild plants, Morus bombycis f. kase, Draba nemorosa var. hebecarpa, Sedum oryzifolium, Lotus corniculatus var. japonicus, and Auricularia auricula-judae 70% ethanol extracts inhibited the viability of tumor cells by 41.85%, 37.31%, 30.29%, 31.98%, and 25.40% at 3 mg/mL of concentration, while inhibition concentration (IC50) values were 1.81, 1.49, 1.05, 1.10, and 0.72 mg/mL, respectively. In Sarcoma 180, NCI H358, and SNU 1 cell lines, the inhibitory activities of A. auricula-judae extract were 65.71%, 69.76%, and 68.01%, respectively. Taken together, the results obtained from the present study indicated that four plant extracts (4% of tested wild plants) and A. auricula-judae extract with similar levels of Ph. Gilvus and G. lucidum extracts may be new potential antitumor agents.

Lotus corniculatus Regulates the Inflammation Induced by Bradykinin in a Murine Model of Pleurisy

This study evaluated the anti-inflammatory efficacy of the crude extract (CE), the fractions derived from hexane (HEX), ethyl acetate (AcOEt), n-butanol (BuOH), and aqueous (Aq) and isolated compounds (oleanolic acid or kaempferitrin) obtained from the aerial parts of Lotus corniculatus var. São Gabriel in mice with bradykinin-induced pleurisy. Swiss mice were used for the In Vivo experiments. Inflammatory parameters [leukocytes; exudate concentrations; myeloperoxidase and adenosine-deaminase activities, and nitric oxide and interleukin-17 levels] were evaluated 4 h after pleurisy induction. The crude extract of Lotus corniculatus, its derived fractions, and isolated compounds inhibited leukocytes and the exudate. This inhibitory effect was associated with decreased of myeloperoxidase and adenosine-deaminase activities, nitric oxide products, and IL-17A levels. Lotus corniculatus presented important anti-inflammatory action by inhibiting leukocyte influx and exudate concentrations. This effect was directly related to the inhibition of nitric oxide and interleukinin17 levels. Oleanolic acid and kaempferitrin can account for these anti-inflammatory effects.

Phylogenetic and functional diversity of alkane degrading bacteria associated with Italian ryegrass (Lolium multiflorum) and Birdsfoot trefoil (Lotus corniculatus) in a petroleum oil-contaminated environment

Twenty-six different plant species were analyzed regarding their performance in soil contaminated with petroleum oil. Two well-performing species, Italian ryegrass (Lolium multiflorum var. Taurus), Birdsfoot trefoil (Lotus corniculatus var. Leo) and the combination of these two plants were selected to study the ecology of plant-associated, culturable alkane-degrading bacteria. Hydrocarbon degrading bacteria were isolated from the rhizosphere, root interior and shoot interior and subjected to the analysis of 16S rRNA gene, the 16S and 23S rRNA intergenic spacer region and alkane hydroxylase genes. Furthermore, we investigated whether alkane hydroxylase genes are plasmid located. Higher numbers of culturable, alkane-degrading bacteria were associated with Italian ryegrass, which were also characterized by a higher diversity, particularly in the plant interior. Only half of the isolated bacteria hosted known alkane hydroxylase genes (alkB and cytochrome P153-like). Degradation genes were found both on plasmids as well as in the chromosome. In regard to application of plants for rhizodegradation, where support of numerous degrading bacteria is essential for efficient break-down of pollutants, Italian ryegrass seems to be more appropriate than Birdsfoot trefoil.

Hydrocarbon degradation and plant colonization by selected bacterial strains isolated from Italian ryegrass and birdsfoot trefoil

To assess the degradation potential and plant colonization capacity of four alkane-degrading strains (ITSI10, ITRI15, ITRH76 and BTRH79) in combination with birdsfoot trefoil and Italian ryegrass and to evaluate the diversity of indigenous alkane-degrading soil bacteria in the rhizo- and endosphere. Contaminated soil was prepared by spiking agricultural soil with 10 g diesel fuel per kg soil. Italian ryegrass (Lolium multiflorum var. Taurus) and birdsfoot trefoil (Lotus corniculatus var. Leo) were inoculated with four alkane-degrading strains. Hydrocarbon degradation (up to 57%) was observed in all inoculated treatments of vegetated and unvegetated samples. Italian ryegrass in combination with compost and BTRH79 showed highest degradation, while birdsfoot trefoil performed best with compost and strain ITSI10. Cultivation-based as well as cultivation-independent analysis showed that both strains were competitive colonizers. The combination between vegetation, inoculation with well-performing degrading bacteria and compost amendment was an efficient approach to reduce hydrocarbon contamination. Two Pantoea sp. strains, ITSI10 and BTRH79, established well in the plant environment despite the presence of a variety of other, indigenous alkane-degrading bacteria. This study suggests that the application of degrading bacterial strains, which are able to compete with the native microflora and to tightly associate with plants, are promising candidates to be used for phytoremediation applications.

Isolation and identification of bioactive compounds responsible for the anti-bacterial efficacy of Lotus corniculatus var. São Gabriel.

Lotus corniculatus (Fabaceae) is considered a forage plant utilized as food for ruminants in the south of Brazil. This herb is also actually used to treat intestinal infection in these animals. In our experiments, we evaluated the anti-bacterial activity of crude extract from L. corniculatus var. Sao Gabriel were assayed against Gram-positive and Gram-negative bacterium. The crude extracted did not show any anti-bacterial activity, but the hexane fraction did on Bacillus cereus (MIC=100 μg/mL) and on Enterococcus faecalis, Listeria monocytogenes, Staphylococcus aureus, Staphylococcus epidermidis, Acinetobacter calcoaceticus, and Providencia alcalifaciens (MIC=600, 800 or 1000 μg/mL). The Ethyl acetate fraction (AcOEt) also showed important anti-bacterial activity on Bacillus cereus, E. faecalis, and Acinetobacter calcoaceticus (MIC=800 μg/mL). The oleanolic acid isolated from hexane fraction showed the same effect on Staphylococcus aureus methycillin-resistant (MIC=100 μg/mL), L. monocytogenes (MIC=25 μg/mL), and Bacillus cereus (MIC=25 μg/mL). Further, Kaempferitrin isolated from ethyl acetate fraction has also shown anti-bacterial activity on Shighella flexinerii (MIC=100 μg/mL), Salmonella typhimurium (MIC=100 μg/mL), A. calcoaceticus (MIC=100 μg/mL), E. faecalis (MIC=3.9 μg/mL), and Bacillus cereus (MIC=8.5 μg/mL). This study suggests that L. corniculatus var. Sao Gabriel have potential pharmacological property for a new anti-bacterial drug development. Key words: Anti-bacterial activity, Kaempferitrin, Lotus corniculatus, Oleanolic acid, β-sitosterol.

Isolation and identification of bioactive compounds responsible for the anti-bacterial efficacy of Lotus corniculatus var. Sao Gabriel

Isolation and identification of bioactive compounds responsible for the anti-bacterial efficacy of Lotus corniculatus var. Sao Gabriel

Evaluation of the anti-inflammatory efficacy of Lotus corniculatus

The anti-inflammatory effects of the crude extract (CE) of Lotus corniculatus var. São Gabriel and its derived hexane (HEX), ethyl acetate (AcOEt), n-butanol (BuOH) and aqueous (Aq) fractions and isolated compounds kaempferitrin, oleanolic acid and β-sitosterol, in a mouse model of pleurisy induced by carrageenan were investigated. Swiss mice were used in the in vivo experiments. The crude extract of L. corniculatus and its derived fractions, and also its isolated compounds, inhibited leukocytes, exudation, and myeloperoxidase (MPO) and adenosine-deaminase (ADA) activities, as well as nitrite/nitrate concentration and interleukin-1 beta (IL-1β) level (p < 0.05). L. corniculatus showed important anti-inflammatory activity by inhibition not only of leukocytes and/or exudation, but also of pro-inflammatory enzymes and mediators such as MPO, ADA, and IL-1β. The constituent’s kaempferitrin, oleanolic acid and β-sitosterol may well account for it.

야생화초지의 조성에 관한 연구

본 연구는 야생화초지 조성을 위한 기초연구로 국내산 야생화초지와 외국산 야생화초지의 2처리를 두어 시험하였다. 공시초종은 기본초종으로 잔디형 목초로 Kentucky bluegrass (Midnight) 를 공시하였고, 국내산 야생화는 패랭이꽃 (Dianthus sinensis L.), 별노랑이 (Lotus corniculatus var. Japonicus Regel), 꼬리풀 (Veronica linariaefolia pallas), 별개미취 (Aster koraiensis Nakai), 부처꽃 (Lythrum anceps Makino), 톱풀 (Achillea sibirica Ledeb), 붓꽃 (Iris nertschinskia Lodd) 및 원추 천인국 (Rudbeckia bicolor Nutt.) 등 8종을 공시하였고, 외국산 야생화는 서양말냉이 (Iberis umbellata), 기생초(Coreopsis tinctoria Nutt), 물망초(Myosotis sylvatica Hoffmann), Baby blue eyes (Nemophila menziesii), 참 제비꽃 (Delphinium ajacis), 아프리칸 데이지 (Dimorphotheca aurantiaca), 왜성 수레국화 (Centaurea cyanus), Dwarf godetia (Clarkia amoena), 왜성 기생초 (Coreopsis tinctoria, dwarf), 왜성 적 기생초(Coreopsis tinctoria, red), Spurred snapdragon (Linaria maroccana), 끈끈이대나물(Silene ameria), 러시안 꽃 무우(Cheiranthus allionii), 캘리포니아 양귀비(Eschscholzia californica Cham), 아이스랜드 양귀비 (Papaver nudicaule), 왜성 제비꽃 (Viola cornuta), Bellflower (Campanula carpatica), Sweet alyssum (Lobularia maritima), 흰 점나도나물 (Cerastium biebersteinii), 큰 달맞이꽃 (Oenothera lamarckiana Ser.), 큰 금계국(Coreopsis lanceolata L.), 각시 패랭이(Dianthus deltoids L.), Baby blue eyes (Nemophila menziesii) 및 참 제비꽃 (Delphinium ajacis) 등 24종을 공시하였다. 시험은 충남대학교 농과대학 초지시험포장에서 2006년 9월부터 2007년 12월까지 수행하였으며, 얻어진 시험결과는 다음과 같다. 야생화초지의 꽃 색깔은 외국산 야생화초지가 국내산 야생화초지보다 다양하였으나 개화가 봄철에 편중되는 등 계절분포 및 영속성이 문제시되었다. 국내산 야생화초지는 꽃 색깔은 다양하지 못하였으나 계절분포 및 영속성이 상대적으로 양호한 것으로 나타났다. 그러나 다년간 식생을 유지하기 위한 야생화 초지의 조성은 기본초종인 잔디형 목초는 물론이고 야생화초종을 지역별로 초기생육 및 개화특성 등을 검토하여 초종을 다양화하는 것이 중요하다고 하겠다. 더욱이 야생화초지를 추파하여 조성할 경우, 외국산 야생화초지는 월동전에는 다양한 식생을 유지하였으나 월동 후에는 내한성의 문제로 식생이 단순화되는 양상을 보였다. 국내산 야생화초지는 내한성에는 문제가 없었으나 식생이 다양화하지 못하였다. 따라서 야생화초지를 다년간 유지할 목적이라면 다년생의 야생화를 중심으로 하되 여기에 단년생을 혼합하여 야생화 초종을 다양화하는 것이 좋을 것으로 사료된다. The purpose of this study was to suggest the basic data for establishment of wildflower pastures. The experimental design includes two treatments: Native wildflower pasture (NWP, Kentucky bluegrass + native wildflower 8 species) and Introduced wildflower pasture (IWP, Kentucky bluegass + introduced wildflower 24 species). The field trials were carried out on the experimental pastures plots at Chungnam National University throughout from Sep. 2006 to Dec. 2007. The results obtained are as fellows: In terms of the flower color in the wildflower pastures, the IWP was found more various than the NWP. But such problems as seasonal distribution and continuity were raised and moreover the time of flowering depended mainly upon the spring season. Even though the flower color in the NWP did not show up its variousness, seasonal distribution and continuity were found comparatively satisfactory. To perennially maintain the botanical composition, the variousness of wildflower species are found important after examining locally the beginning rearing and flowering characteristics of wildflowers as well as the basic turfgrass. Moreover, when the wildflower pastures was constructed in autumn, since most of the introduced wildflower were annuals, it maintained various botanical composition before wintering, but after wintering the botanical composition were found simplified due to the winter tolerance. The NWP did not show any problems in the winter tolerance but it could not make the botanical composition diversified. Accordingly, to maintain the wildflower pasture for many years, diversifying wildflower species based upon perennials including annuals seems to be good.

야생화초지 혼파조합을 위한 몇 가지 잔디형 및 야생화 초종의 초기생육과 개화특성에 관한 연구

본 연구는 야생화 초지조성을 위한 잔디형 목초 및 야생화초종의 선정과 혼파조합구성에 대한 기초자료를 탐색하고자 몇 가지 잔디형 목초와 야생화초종을 공시하여 초기생육, 경엽 및 근중, 건물수량, 개화특성 및 기호성 등을 비교분석하였다. 공시초종은 잔디형 목초 5종{Kentucky bluegrass(Midnight), tall fescue(Millennium), perennial ryegrass(Palmer III), redtop(Barricuda) 및 creeping bentgrass(Crenshaw)}과 야생화 11종{Chrysanthemum leucanthemum L., Coreopsis lanceolata L., Rudbeckia bicolor Nutt., Dianthus chinensis L., Chrysanthemum lindicum L., Lotus corniculatus var. Japonicus Regel, Veronica linariaefolia Pallas, Aster koraiensis Nakai., Chrysanthemum zanadskii var. latilobum(Maxim.) Kitamura, Lythrum anceps Makino 및 Iris nertschinskia. Lodd}을 공시하였으며, 충남대학교 농과대학 초지시험포장에서 2005년 1월부터 2006년 12월까지 수행하였다. 공시된 잔디형 목초 중에서 상대적으로 야생화와 혼파가능성이 큰 초종은 Kentucky bluegrass이었으며, Kentucky bluegrass에 비하여 상대적으로 초기생육이 왕성하고 경엽과 근중이 높아 야생화초지를 조성하는 데 유리할 것으로 보이는 야생화는 Chrysanthemum leucanthemum L., Lotus corniculatus var. Japonicus Regel, Chrysanthemum zawadskii var. latilobum (Maxim) Kitamura 및 Lythrum anceps Makino 등으로 조사되었다. 이들 야생화는 꽃색, 개화시기 및 개화지속기간 등이 서로 중복되지 않았으며 가축에 의한 기호성도 대체적으로 양호한 결과를 보였다. 따라서 야생화초지의 조성을 위해서는 잔디형 목초는 야생화와의 경합력이 낮은 초종을, 반대로 야생화는 경합에서 유리한 초종을 선발하되 개화특성이 서로 다른 야생화를 다양화하여 혼파조합을 구성하는 것이 중요하다고 판단된다. The purpose of this study was to compared the early growth pattern, stem, leaf and root weight, DM yield, flowering characteristics and preference for selection of turf type grasses and wild flower species in mixtures combination for wildflower pasture. The experimental design included five species of turf grasses and 11 species of wildflower : turf type grass species{Kentucky bluegrass(Midnight), tall fescue(Millennium), perennial ryegrass(Palmer III), redtop(Barricuda) and creeping bentgrass(Crenshaw)}, wildflower species {Chrysanthemum leucanthemum L. Coreopsis lanceolata L., Rudbeckia bicolor Nutt.., Dianthus chinensis L., Chrysanthemum lindicum L., Lotus corniculatus var. Japonicus Regel, Veronica linariaefolia Pallas, Aster koraiensis Nakai., Chrysanthemum zanadskii var. latilobum (Maxim.) Kitamura, Lythrum anceps Makino and Iris pallassi var. chinensis Lodd}. The field trials were carried out on the experimental pasture plots of Chungnam National University from January 2005 to December 2006. The appropriate species of turf grass which have a mixture possibility with wildflower are thought to be Kentucky bluegrass. Compared with Kentucky bluegrass, on the other hand, species such as Chrysanthemum leucanthemum L., Lotus corniculatus var. Japonicus Regel, Chrysanthemum zanadskii var. latilobum Maxim.) Kitamura and Lythrum anceps Makino have shown advantageous aspects of fast early growth pattern and highly weights of stem, leaf and root. Futhermore, these wild flowers were in harmony and no overlap on flower color, flowering periods and flowering longevity, and highly preference by Korean native goats. For the establishment of wildflower pasture in Korea, therefore, it is important to select the appropriate species of turf glasses, which have a weak competition against wild flowers, and then to make mixture combination of those turf glasses with the various wild flowers which have a strong competition against turf glasses but have a different anthesis characteristics among themselves.

Characterization of Condensed Tannins Purified From Legume Forages: Chromophore Production, Protein Precipitation, and Inhibitory Effects on Cellulose Digestion

To identify simple screening tools for selecting condensed tannin (CT)-containing forages as candidate sources for further study, CT were isolated from nine legumes, and their molecular weights (MW), chromophore production, capacity to precipitate bovine serum albumin (BSA) and Fraction 1 protein (Rubisco) isolated from alfalfa, and inhibition of filter paper digestion were compared. Sources were as follows: leaves of sericea lespedeza (Lespedeza cuneata Dum.-Cours.), crown vetch (Coronilla varia L.), and sainfoin (Onobrychis viciifolia Scop.); stems of hedysarum (Hedysarum alpinum L.); seeds of alfalfa (Medicago sativa L.); and whole plants of birdsfoot trefoil (Lotus corniculatus var. corniculatus L.) and three varieties of big trefoil (Lotus pedunculatus Cav.), viz., Lotus uliginosus Schkuhr, L. uliginosus var. glabriusculus, and L. uliginosus var. villosus. Molecular weights and sizes (degrees of polymerization) of the CT varied considerably within and among plant species. Average MW ranged from 3036 Da (crown vetch) to 7143 Da (lespedeza). All CT exhibited greater capacity (w/w basis) to bind alfalfa Rubisco than BSA. Relative astringencies (microg CT required to precipitate 1 mg protein) against BSA ranged from 262.5 for CT from lespedeza to 435.5 for CT from L. corniculatus, and against Rubisco, from 49.6 (sainfoin) to 108.2 (alfalfa seed). Including CT at 300 microg/ml in cultures of Fibrobacter succinogenes reduced digestion of cellulose filter paper by 19.8% (sainfoin) to 92.4% (crown vetch) and increased the specific activity of cell-associated endoglucanase. There were no correlations between inhibitory effects of CT on filter paper digestion and (1) chromophore formation during CT assay by butanol-HCl, vanillin-HCl, or H2SO4; (2) precipitation of BSA or alfalfa Rubisco; and (3) MW of CT. The most inhibitory CT for cellulose digestion included those with broad and with narrow MW distributions. Sainfoin was the most desirable source of CT, as it had the highest capacity to bind alfalfa protein and was least inhibitory to cellulose digestion by F. succinogenes. This study suggests that these properties are not easily defined via chemical means, and that biological assays using rumen bacteria may help identify those CT with properties of nutritional interest.

Fate of Plants from Buried Seeds on Volcano Usu, Japan, after the 1977-1978 Eruptions

The eruptions of 1977–1978 on Mount Usu in northern Japan resulted in the almost complete destruction of vegetation by a 1–3-m-thick accumulation of volcanic deposits. Erosion created gullies that removed these deposits and frequently exposed the old original soil. I previously confirmed that seedlings of 14 species (eight annual and six perennial herbs) emerged from seeds buried in the original topsoil. To clarify the role of the seed bank on volcanic succession, I monitored seedlings of seed bank species from 1983 to 1992. Nearly all the annuals, such as Polygonum longisetum and Rorippa islandica, originated from the seed bank. However, seed supply from the seed bank declined with time. Because seedling mortality was extremely high and reproductive success was low, due mostly to the instability of ground surface, these annuals emerged for several years but disappeared after 1989. The nitrogen-fixing and stoloniferous perennials, Trifolium repens and Lotus corniculatus var. japonicus, were derived only from the seed bank. They have gradually increased in cover and have become large enough to flower. The other perennials derived from the seed bank, most of which produce short rhizomes, did not increase in cover annually and thus contributed less to revegetation than nitrogen-fixing stoloniferous species. The fate of perennials seems to be dependent on the types of rhizome and stolon systems. Although recruitment from the seed bank was restricted to gullies, the seed bank was the major source of annuals and of nitrogen-fixing perennials in the gullies. Succession in the gullies was substantially different from that which occurred outside the gullies where plants mostly originated from seed immigration and vegetative reproduction from surviving plants, and annual and nitrogen-fixing plants were absent.