Cornell Net Carbohydrate And Protein System Research Articles

Protein and Carbohydrate Fractionation to Evaluate Perennial Ryegrass (Lolium perenne L.) Accessions

Precise information about carbohydrates and proteins in relation to their utilization in the rumen is useful for the breeding purposes of perennial ryegrass cultivars used for animal nutrition. The objective of the current study was to evaluate 20 diploid perennial ryegrass accessions from the intermediary heading stage. The ruminal kinetics of different carbohydrate and protein fractions of grasses and legumes are important for forage breeding programs. The Cornell Net Carbohydrate and Protein System (CNCPS) was used to provide such information. Accession-based variation can be observed after considering dynamic degradation processes. Variation among the 20 accessions was observed. Ruminally digested (RDC) and undigested (UDC) carbohydrate and ruminally degraded (RDP) and undegraded (UDP) protein contents, total carbohydrate and total protein contents, and carbohydrate and protein fractions for the first cut and annual averages show significant differences. Although the variation was smaller for the protein fractions, the calculated usable protein content (uCP, sum of UDP and amount of synthesized microbial protein in the rumen) in the small intestine is mainly influenced by decreasing the neutral detergent fiber (NDF) and increasing the sugar content among cultivars. Carbohydrate and protein fractionation is suitable for characterizing perennial grass accessions as it uses parameters relevant to ruminant nutrition, allowing a step forward in forage plant breeding for forage quality. To conclude, using parameters related to ruminal degradation kinetics should favor the selection of accessions with higher amounts of ruminally digested carbohydrates (RDC). The selection of accessions based on protein quality (proportion of UDP) is less favorable for achieving a higher usable protein content.

Effect of growing regions on morphological characteristics, protein subfractions, rumen degradation and molecular structures of various whole-plant silage corn cultivars.

Little information exists on the variation in morphological characteristics, nutritional value, ruminal degradability, and molecular structural makeup of diverse whole-plant silage corn (WPSC) cultivars among different growing regions. This study investigated the between-regions (Beijing, Urumchi, Cangzhou, Liaoyuan, Tianjin) discrepancies in five widely used WPSC cultivars in China (FKBN, YQ889, YQ23, DK301 and ZD958), in terms of 1) morphological characteristics; 2) crude protein (CP) chemical profile; 3) Cornell Net Carbohydrate and Protein System (CNCPS) CP subfractions; 4) in situ CP degradation kinetics; and 5) CP molecular structures. Our results revealed significant growing region and WPSC cultivar interaction for all estimated morphological characteristics (P < 0.001), CP chemical profile (P < 0.001), CNCPS subfractions (P < 0.001) and CP molecular structural features (P < 0.05). Except ear weight (P = 0.18), all measured morphological characteristics varied among different growing regions (P < 0.001). Besides, WPSC cultivars planted in different areas had remarkably different CP chemical profiles and CNCPS subfractions (P < 0.001). All spectral parameters of protein primary structure of WPSC differed (P < 0.05) due to the growing regions, except amide II area (P = 0.28). Finally, the area ratio of amide I to II was negatively correlated with the contents of soluble CP (δ = -0.66; P = 0.002), CP (δ = -0.61; P = 0.006), non-protein nitrogen (δ = -0.56; P = 0.004) and acid detergent insoluble CP (δ = -0.43; P = 0.008), in conjunction with a positive correlation with moderately degradable CP (PB1; δ = 0.58; P = 0.01). In conclusion, the cultivar of DK301 exhibited high and stable CP content. The WPSC planted in Beijing showed high CP, SCP and NPN. The low rumen degradable protein of WPSC was observed in Urumchi. Meanwhile, above changes in protein profiles and digestibility were strongly connected with the ratio of amide I and amide II.

Optimizing corn silage quality during hot summer conditions of the tropics: investigating the effect of additives on in-silo fermentation characteristics, nutrient profiles, digestibility and post-ensiling stability

Corn crop grown and ensiled at high temperature have lower water soluble carbohydrates (WSC), epiphytic lactic acid bacteria (LAB) population, lactic acid concentration, fermentation quality and aerobic stability. This study systematically investigated the effects of heterofermentative LAB (hetLAB), homofermentative LAB (homLAB), molasses and their mixture (MIX) on in-silo fermentation characteristics, chemical profiles, Cornell Net Carbohydrate and Protein System (CNCPS) carbohydrate subfractions, in vitro digestibility (DMD), microbial count, and post-ensiling aerobic stability of whole crop corn silage during hot summer (30 to 45°C) condition. Corn hybrids (P30K08 and DK6789) were ensiled at targeted dry matter (DM) of 330 g/kg for 0, 3, 7, 21, and 150 days in 3 L silos, without additive (CCS) or treated with hetLAB (4×106 cfu/g Lactobacillus buchneri), homLAB (1×106 cfu/g of L. plantarum), molasses (3% of fresh forage) or MIX (half of individual doses of homLAB, hetLAB and molasses) additives. The CCS, homLAB, hetLAB, molasses, or MIX treated chopped material of each hybrid were ensiled in 16 replicate silos at a density of 260 kg of DM/m3. Compared to CCS, the additives significantly improved silage nutritional and fermentation quality, DM digestibility (in vitro), count of LAB, DM recovery and aerobic stability, and decreased counts of yeast and mold. Among the inoculants, the homLAB and MIX inoculated silages had greatest improvement in fermentation quality and nutritional value. The homLAB produced corn silage with the highest (P &amp;lt; 0.05) content of lactic acid, and soluble carbohydrates, and lowest contents of acetic acid, NH3-N and pH, demonstrating desirable and restricted in silo fermentation. On the other hand, the hetLAB inoculated silages had the greatest (P &amp;lt; 0.05) value of acetic acids, highlighting greater aerobic stability. Interestingly, the MIX silages followed the hetLAB in acetic acid value and homLAB in lactic acid value. Notably, without additive stable pH was not achieved during 21 days, with application of molasses, hetLAB and the MIX inoculants stable pH was achieved during 7 days, and with homLAB stable pH was achieved during the first 3 days of ensiling. The greatest numbers of viable LAB were recorded in homLAB (8.13 log cfu/g) and MIX (7.89 log cfu/g) inoculated silages, while the lowest for CCS (6.29 log cfu/g). The lowest yeast (1.48 log cfu/g) and mold (0.22 log cfu/g) were recorded for hetLAB inoculated silage. The greatest (P &amp;lt; 0.05) DM recovery was recorded for hetLAB (97.3%) and MIX (96.9%), and the lowest for the control silage (92.9%). All additives significantly improved the aerobic stability of corn silage, and the greatest value of &amp;gt;72 h was recorded for hetLAB and MIX inoculats, and the lowest for CSC (25 h). In conclusion, additives application can improve fermentation quality, nutritional value, DM recovery and aerobic stability of whole crop corn silage under hot summer conditions of the tropics. The MIX inoculant showed potential to improve in-silo fermentation, and aerobic stability at the same time, however, further investigation are required, particularly with respect of dose rate.

In vitro protein fractionation methods for ruminant feeds

Estimating protein fractions and their degradation rate are vital to ensure optimum protein supply and degradation in the digestive system of ruminants. This study investigated the possibility of using the ANKOM gas production system and preserved rumen fluid to estimate the protein fractions and in vitro degradability of protein-rich feeds. Three in vitro methods: (1) gas production method (2) Cornell Net Carbohydrate and Protein System (CNCPS), and (3) the unavailable nitrogen assay of Ross (uNRoss) were used to quantify protein fractions of four feeds (lupin meal, vetch grain, Desmanthus hay, and soybean meal). Rumen fluid mixed with 5% dimethyl sulfoxide and frozen at −20 °C was also compared against fresh rumen fluid in the gas production and uNRoss methods. All three methods ranked the feeds identically in the proportions of available (degradable or ‘a + b’) protein fractions as vetch grain, soybean meal, lupin meal, and Desmanthus hay in decreasing order. The use of fresh rumen fluid produced greater available protein fractions than preserved rumen fluid in all feeds. However, there was no difference between total gas production from lupin meal and vetch grain fermented for 16 h in either rumen fluid source. The in vitro degradable CP (IVDP) was higher for vetch grain (46 and 70%) at the 4th and 8th hours of incubation than other feeds, whereas soybean meal (85%) exceeded the other feeds after the 16th hour of incubation (P < 0.001). The greatest ammonia-N concentration was from soybean meal (1.27 mg/g) and lupin meal (0.87 mg/g) fermented for four hours using fresh rumen fluid. The proportion of fraction 'b' for soybean (82.1% CP) and lupin meals (39.4% CP) from the CNCPS method were not different (P = 0.001) from the fraction 'b' estimation of the gas production method for the same feeds (r = 0.99). Regardless of the methods, a greater water-soluble protein fraction was found from vetch grain (39.6–46.6% CP), and the proportion of fraction ‘c’ or unavailable protein in Desmanthus hay (39.1–41.5% CP) exceeded other substrates (P < 0.001). The strong positive correlation between fractions across different methods and identical ranking of feeds suggests the possibility of using ANKOM gas production apparatus for protein fractionation.

Predicting orthophosphate in feces and manure from dairy cattle

Dairy cattle excreta are a valuable source of orthophosphate (Ortho-P), an inorganic form of phosphorus (P) that is readily available for microorganisms, plant growth, and development. There is, however, a growing environmental concern about the potential negative environmental impact of excessive amounts of Ortho-P excretion, which can lead to the eutrophication of water bodies. As a result, the development of mathematical equations to quantify and manage Ortho-P excretion on dairy farms could prove valuable for environmental sustainability. This study aimed to use literature data to develop empirical predictions for Ortho-P (g/kg dry matter [DM]) excretion using total P (TP [g/kg DM]) content of dairy cattle feces (Ortho-Pf) and manure (Ortho-Pm). Data sets from studies that evaluated and characterized the different forms of P in feces and manure from dairy cattle were compiled. After outlier exclusion, the final retained database for feces included 37 treatment means from 4 published papers while the manure comprised 23 treatment means from 7 published papers. A linear-mixed model was used to develop the predictive equations, incorporating the random effect of the study. A leave-one-out cross-validation procedure was used to evaluate the predictive ability of the developed models, whereby studies were regarded as folds. The fecal equation was determined as Ortho-Pf (g/kg DM) = −2.447 (0.572) + 0.966 (0.083) × TP (g/kg DM) (R2 = 0.79) and resulted in a root mean square prediction error as a percentage of mean observed value (RMSPE, %) of 32.8% and error due to random sources of 97.6%. Additionally, the manure equation was determined as Ortho-Pm (g/kg) = −0.204 (0.446) + 0.590 (0.065) × TP (g/kg) (R2 = 0.77) and had an RMSPE of 43.3% with a random error of 93.9%. Both models revealed minimal mean and slope biases on feces and manure data. Findings suggest that these sets of equations can be used to estimate excreted Ortho-P from total excreted P, helping nutritionists and farmers to understand the impact of dietary P changes on the environment. Further, these equations can be incorporated into extant models such as the Cornell Net Carbohydrate and Protein System (CNCPS) to aid in understanding and mitigating P and Ortho-P excretion from dairy cattle and to clarify the portion of P that migrates more rapidly into watersheds.

Effect of Mixing Peanut Vine on Fermentation Quality, Nitrogen Fraction and Microbial Community of High-Moisture Alfalfa Silage

Fresh alfalfa is difficult to ensile successfully because of its high moisture content and greater susceptibility to spoilage by Clostridia, Bacilli or Enterobacter. In this study, we evaluated the effects of mixing high-moisture alfalfa with peanut vine in different proportions on the bacterial communities and fermentation characteristics of silage. The high-moisture alfalfa and peanut vine were mixed at ratios of 10:0 (CK), 8:2 (TI), 7:3 (T2), 6:4 (T3) and 5:5 (T4), respectively. For each treatment, silos (25 × 35 cm) were anaerobically fermented in darkness at room temperature and analyzed after 45 days. The results showed that the CK silage was weakly fermented, as indicated by a low lactic acid concentration, a high pH value, and high levels of propionic acid (PA), butyric acid (BA) and ammonia nitrogen (NH3-N). As the proportion of peanut vine in the mixture increased, the pH level decreased, and levels of BA, propionic acid, NH3-N, crude protein(CP), nonprotein nitrogen and soluble protein also declined (p &lt; 0.05), while the lactic acid concentration increased and levels of neutral detergent fiber (NDF) and water-soluble carbohydrates (WSC) also rose (p &lt; 0.05). A protein component analysis of silage mixtures using the Cornell Net Carbohydrate and Protein System (CNCPS) showed that the content of the nonprotein nitrogen component (PA) decreased when the proportion of peanut vine increased, whereas the content of rapidly degraded protein (PB1) increased. Mixing with peanut vine also influenced the distribution of the bacterial community. Compared with the CK silage, the relative abundances of Enterococcus, Garciella and Anaerosporobacter in T2, T3 and T4 were significantly lower, while the relative abundance of Lactobacillus was significantly higher. In the T2, T3 and T4 groups, Garciella and Anaerosporobacter were not detected. In summary, in this study, we ensiled high-moisture alfalfa, which was weakly fermented. We found that mixing with peanut vine improved fermentation quality and optimized the structure of the bacterial community. Therefore, to improve the fermentation quality and nutritional value of silage, high-moisture alfalfa should be ensiled with at least 30% peanut vine.

Impact of processing methods (dry-heating, autoclaving, and (microwave irradiation) on protein-related molecular structure spectral feature and protein nutritive value of cool-seasoned oat varieties in ruminant system in western Canada

The objectives of this study were to reveal the effects of heating method and variety on (1) protein molecular structure spectral features revealed by Attenuated Total Reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and (2) ruminant relevant protein chemical profiles, protein subfractions, rumen degradable protein (RDP), rumen undegradable protein (RUP), and intestinal protein digestibility using the Cornell Net Carbohydrate and Protein System (CNCPS) in oat varieties [CDC Nasser (feed-type oat) and CDC Seabiscuit (milling-type oat)]. Heating method included control (raw), dry heating (DH), moist heating (autoclaving, MH), and microwave irradiation (MIR). The dry matter (DM), crude protein (CP), and neutral detergent fiber (NDF) contents in CDC Nasser were 934, 107, and 252 g/kg DM, respectively; the contents of these nutrients in CDC Seabiscuit were 933, 110, and 268 g/kg DM, respectively. Heat processing significantly affected the total RDP (TRDP, P < 0.001), which was higher for DH than MIR and MH in CDC Nasser (5.46%, 5.01%, and 4.45%DM). Conversely, MIR had a higher value for TRDP than DH and MH in CDC Seabiscuit (5.32%, 5.24%, and 4.51%DM). The total RUP (TRUP) was higher for MH than MIR and DH in both varieties (P < 0.001). The intestinal digestible feed protein was similar between the varieties (P = 0.308); however, DH and MH reduced the value, while MIR increased it compared to the control (P = 0.010) for both varieties. The results from the ATR-FTIR analysis showed an interaction between varieties and heat processing in the protein secondary structures α-helix (P = 0.003), β-sheet (P = 0.031), and their ratio (P = 0.030). Moreover, in the multivariate analyses, no independent groups were observed between the raw and the three heat processing methods in the protein-related molecular structure of oats. In conclusion, heat processing altered the protein subfractions, the TRDP, and TRUP, affecting intestinal protein digestibility. The ATR-FTIR spectroscopy with univariate molecular analysis showed heat processing-induced molecular structure changes in the protein-related spectral profiles in CDC Nasser and CDC Seabiscuit.

Effect of Metabolizable Protein Supply on Milk Performance, Ruminal Fermentation, Apparent Total-Tract Digestibility, Energy and Nitrogen Utilization, and Enteric Methane Production of Ayrshire and Holstein Cows

In North America, the nutrient requirements of dairy cattle are predicted using the Cornell Net Carbohydrate and Protein System (CNCPS) or the National Research Council (NRC). As Holstein is the most predominant dairy cattle breed, these models were developed based on the phenotypic, physiological, and genetic characteristics of this breed. However, these models may not be appropriate to predict the nutrient requirements of other breeds, such as Ayrshire, that are phenotypically and genetically different from Holstein. The objective of this study was to evaluate the effects of increasing the metabolizable protein (MP) supply using CNCPS on milk performance, ruminal fermentation, apparent total-tract digestibility, energy and N utilization, and enteric methane production in Ayrshire vs. Holstein lactating dairy cows. Eighteen (nine Ayrshire; nine Holstein) lactating cows were used in a replicated 3 × 3 Latin square design (35-d periods) and fed diets formulated to meet 85%, 100%, or 115% of MP daily requirement. Except for milk production, no breed × MP supply interaction was observed for the response variables. Dry matter intake (DMI) and the yields of energy-corrected milk (ECM), fat, and protein were less (p < 0.01) in Ayrshire vs. Holstein cows. However, feed efficiency and N use efficiency for milk production did not differ between the two breeds, averaging 1.75 kg ECM/kg DMI and 33.7 g milk N/100 g N intake, respectively. Methane yield and intensity and urinary N also did not differ between the two breeds, averaging 18.8 g CH4 /kg DMI, 10.8 g CH4 /kg ECM, and 27.6 g N/100 g N intake, respectively. Yields of ECM and milk protein increased (p ≤ 0.01) with increasing MP supply from 85% to 100% but no or small increases occurred when MP supply increased from 100 to 115%. Feed efficiency increased linearly with an increasing MP supply. Nitrogen use efficiency (g N milk/100g N intake) decreased linearly (by up to 5.4 percentage units, (p < 0.01) whereas urinary N excretion (g/d or g/100 g N intake) increased linearly (p < 0.01) with an increasing MP supply. Methane yield and emission intensity were not affected by MP supply. This study shows that feed efficiency, N use efficiency, CH4 (yield and intensity), and urinary N losses did not differ between Ayrshire and Holstein cows. Energy-corrected milk yield and feed efficiency increased, but N use efficiency decreased and urinary N losses increased with increasing dietary MP supply regardless of breed. Ayrshire and Holstein breeds responded similarly to increasing MP levels in the diet.

Balancing dairy cattle diets for rumen nitrogen and methionine or all essential amino acids relative to metabolizable energy

Improving the ability of diet formulation models to more accurately predict AA supply while appropriately describing requirements for lactating dairy cattle provides an opportunity to improve animal productivity, reduce feed costs, and reduce N intake. The goal of this study was to evaluate the sensitivity of a new version of the Cornell Net Carbohydrate and Protein System (CNCPS) to formulate diets for rumen N, Met, and all essential AA (EAA). Sixty-four high-producing dairy cattle were randomly assigned to 1 of the 4 following diets in a 14-wk longitudinal study: (1) limited metabolizable protein (MP), Met, and rumen N (Base), (2) adequate Met but limited MP and rumen N (Base + M), (3) adequate Met and rumen N, but limited MP (Base + MU), and (4) adequate MP, rumen N, and balanced for all EAA (Positive). All diets were balanced to exceed requirements for ME relative to maintenance and production, assuming a nonpregnant, 650-kg animal producing 40 kg of milk at 3.05% true protein and 4.0% fat. Dietary MP was 97.2, 97.5, 102.3, and 114.1 g/kg of dry matter intake for the Base, Base + M, Base + MU, and Positive diets, respectively. Differences were observed for dry matter intake and milk yield (24.1 to 24.7 and 39.4 to 41.1 kg/d, among treatments). Energy corrected milk, fat, and true protein yield were greater (2.9, 0.13, and 0.08 kg/d, respectively) in cows fed the Positive compared with the Base diet. Using the updated CNCPS, cattle fed the Base, Base + M, and Base + MU diets were predicted to have a negative MP balance (-231, -310, and -142 g/d, respectively), whereas cattle fed the Positive diet consumed 33 g of MP/d excess to ME supply. Bacterial growth was predicted to be depressed by 16 and 17% relative to adequate N supply for the Base and Base + M diets, respectively, which corresponded with the measured lower apparent total-tract NDF degradation. The study demonstrates that improvements in lactation performances can be achieved when rumen N and Met are properly supplied and further improved when EAA supply are balanced relative to requirements. Formulation using the revised CNCPS provided predictions for these diets, which were sensitive to changes in rumen N, Met, all EAA, and by extension MP supply.

Effect of Spring Maize Genotypes on Fermentation and Nutritional Value of Whole Plant Maize Silage in Northern Pakistan

In the current study, we evaluated the growth, biomass yield, ensiling, and nutritional characteristics of spring maize genotypes grown under the climatic conditions of Northern Pakistan. Six promising spring maize genotypes were grown under uniform standard agronomic conditions in 72 plots (8 m × 10 m), blocked within three replicate fields. Maize crops were harvested at targeted dry matter (DM) content (33 g/100 g DM). Data were collected on plant phonological characteristics and biomass yield, and then the harvested crop of each plot was chopped, and subsamples were ensiled in three replicate 1.5 L laboratory silos (n = 12/genotype). After 90 days of ensiling, subsamples were analyzed for fermentation quality, nutrient composition, Cornell Net Carbohydrate and Protein System (CNCPS) carbohydrate subfractions, digestible nutrients, metabolizable energy (ME), and in vitro dry matter digestibility (DMD). Results revealed large differences (p &lt; 0.001) among maize genotypes in the yields (tons/ha) of DM (13.0 to 17.9), crude protein (CP; 0.83 to 1.24), and starch (4.16 to 6.67). Except for total carbohydrates (CHO) and NH3-N, the contents of all measured chemical components varied (p &lt; 0.001) among the spring maize genotypes. Similarly, all reported CNCPS subfractions varied (p &lt; 0.01) among the genotypes, except for the non-digestible (CC) subfraction. Among the genotypes, there were large variations in the contents (g/100 g DM) of CP (6.60 to 8.05), starch (32.0 to 37.3), non-fiber carbohydrates (40.3 to 43.8), in vitro DMD (61.5 to 67.9), total digestible nutrients (68.1 to 70.6), digestible energy (2.80 to 3.07 Mcal/kg), and metabolizable energy (2.28 to 2.44 Mcal/kg), with genotype QPM300 having the highest values and genotype Azam having the lowest values. It was concluded that QPM300 is the most suitable spring maize genotype for silage production in terms of yields and silage nutritional and fermentation quality under the environmental condition of Northern Pakistan.

Carbohydrate and Protein Fractionations of commonly used forages and agro-industrial byproducts as per Cornell Net Carbohydrate and Protein system (CNCPS)

Carbohydrate and Protein Fractionations of commonly used forages and agro-industrial byproducts as per Cornell Net Carbohydrate and Protein system (CNCPS)

Protein and carbohydrate profiles of a diploid and a tetraploid red clover cultivar

Red clover (Trifolium pratense L.) is characterized by high dry matter (DM) yield and a high nutritive value. It has a higher concentration of soluble carbohydrate than lucerne and its protein is less degradable in the rumen than lucerne protein. The aim of this study was to quantify the main crude protein (CP) and carbohydrate (CHO) fractions of a tetraploid (4n, cv K-32) and a diploid (2n, cv K-39) red clover cultivar harvested at three stages of development in the spring growth. Results showed that the cultivar and stage of development had a large impact on chemical composition and the protein and carbohydrate fractions determined by the Cornell Net Carbohydrate and Protein System (CNCPS). The rapidly degradable protein fraction (PA) was significantly higher in the tetraploid (272 to 287 g kg-1 CP with advancing plant development) than in the diploid red clover cultivar (214 to 268 g kg-1 CP). Rumen degradable carbohydrate concentration was not affected by cultivar but it was significantly influenced by the stage of development. The concentration of rumen degradable protein decreased from 726 to 655 g kg-1 CP with advancing plant development in the diploid red clover cultivar. In conclusion, the tetraploid red clover cultivar was higher in CP, its protein was more rapidly degradable, and it was characterized by lower concentrations of lignin and unavailable carbohydrate fractions than the diploid cultivar.

Quantitation of relationship and development of nutrient prediction with vibrational molecular structure spectral profiles of feedstocks and co-products from canola bio-oil processing.

This program aimed to reveal the association of feed intrinsic molecular structure with nutrient supply to animals from canola feedstocks and co-products from bio-oil processing. The special objective of this study was to quantify the relationship between molecular spectral feature and nutrient availability and develop nutrient prediction equation with vibrational molecular structure spectral profiles. The samples of feedstock (canola oil seeds) and co-products (meals and pellets) from different bio-oil processing plants in Canada (CA) and China (CH) were submitted to this molecular spectroscopic technique and their protein and carbohydrate related molecular spectral features were associated with the nutritional results obtained through the conventional methods of analyses for chemical and nutrient profiles, rumen degradable and intestinal digestible parameters. The results showed that the spectral structural carbohydrates spectral peak area (ca. 1,487.8 to 1,190.8 cm-1) was the carbohydrate structure that was most significant when related to various carbohydrate parameters of canola meals (p<0.05, r>0.50). And spectral total carbohydrate area (ca. 1,198.5 to 934.3 cm-1) was most significant when studying the various carbohydrate parameters of canola seeds (p<0.05, r>0.50). The spectral amide structures (ca. 1,721.2 to 1,480.1 cm-1) were related to a few chemical and nutrient profiles, Cornell Net Carbohydrate and Protein System (CNCPS) fractions, truly absorbable nutrient supply based on the Dutch protein system (DVE/OEB), and NRC systems, and intestinal in vitro protein-related parameters in co-products (canola meals). Besides the spectral amide structures, α-helix height (ca. 1,650.8 to 1,643.1 cm-1) and β-sheet height (ca. 1,633.4 to 1,625.7 cm-1), and the ratio between them have shown to be related to many protein-related parameters in feedstock (canola oil seeds). Multiregression analysis resulted in moderate to high R2 values for some protein related equations for feedstock (canola seeds). Protein related equations for canola meals and carbohydrate related equations for canola meals and seeds resulted in weak R2 and low p values (p<0.05). In conclusion, the attenuated total reflectance Fourier transform infrared spectroscopy vibrational molecular spectroscopy can be a useful resource to predict carbohydrate and protein-relates nutritional aspects of canola seeds and meals.

Protein and carbohydrate fractionation of silages made from maize, &lt;i&gt;Urochloa&lt;/i&gt; species and their mixtures

New feed assessment systems and methodologies for ruminants are being used with the aim of maximizing the use of nutrients by animals. The Cornell Net Carbohydrate and Protein System (CNCPS) considers the dynamics of ruminal fermentation and the potential loss of nitrogen in feed evaluation. We used this system to evaluate the protein and carbohydrate fractionation of silages made from maize and Urochloa species alone and in combination (70:30). The experiment was carried out under a completely randomized experimental design with 4 replications. Treatments comprised silages made from the following forages: maize (Zea mays); Congo grass (Urochloa ruziziensis); Xaraes palisadegrass (U. brizantha 'Xaraés'); Paiaguas palisadegrass (U. brizantha 'BRS Paiaguás'); 70% maize + 30% Congo grass; 70% maize + 30% Xaraes palisadegrass; and 70% maize + 30% Paiaguas palisadegrass. The results showed that despite the Urochloa exclusive silages having higher crude protein concentration than maize and mixed silages, they have a higher proportion of unavailable fractions with slow degradation rates, which can compromise animal performance. The maize silage and mixed silages had higher percentages of protein and carbohydrates with high degradation potential than Urochloa exclusive silages. Therefore, mixed silages represent one more alternative to provide forage with good nutritional value for ruminant feeding in times of feed shortage. Mixing grass and maize at ensiling would increase the volume of silage produced relative to ensiling maize alone without any significant reduction in quality of the silage produced. However, further studies are needed to determine the appropriate combinations of maize and grass at ensiling to produce silage with the desired nutritional value for the particular application and class of animals being fed. Feeding studies with animals would verify production levels achieved with the various silages.

Associations of Protein Molecular Structures with Their Nutrient Supply and Biodegradation Characteristics in Different Byproducts of Seed-Used Pumpkin.

Simple SummaryAt present, the world is faced with a shortage and rising prices of high-quality protein feed. Adequate utilization of unconventional feeds can alleviate this problem. China is the largest producer and exporter of seed-used pumpkin, and many byproducts of seed-used pumpkin need to be effectively utilized. In this study, we analyzed nutrient supply and biodegradation characteristics, and correlation and regression equations between them and protein molecular structure were established. This research provides a data basis for the utilization of byproducts of seed-used pumpkin and establishes a new method for evaluating the nutritional value of unconventional feeds.The purpose of this experiment was to explore the relationship of protein functional groups (including amide I, amide II, α-helix, and β-sheet) in byproducts of seed-used pumpkin (pumpkin seed cake, pumpkin seed coat, and seed-used pumpkin flesh) with their nutrient profiles and biodegradation characteristics. The experiment was designed to use conventional chemical analysis, combining the Cornell Net Carbohydrate and Protein System (CNCPS) and nylon bag technology to assess the nutritional value and biodegradation characteristics of seed-used pumpkin byproducts. Fourier transform infrared spectroscopy (FTIR) was used to analyze the protein molecular structure properties of byproducts of seed-used pumpkin. In this study, we also applied correlation and regression analysis. The results showed that different byproducts of seed-used pumpkin had different in situ biodegradation, nutrient supplies, and spectral structures in the protein region. Among the byproducts of seed-used pumpkin, acid detergent-insoluble crude protein (ADICP) and neutral detergent-insoluble crude protein (NDICP) contents of the pumpkin seed coat were the highest, resulting in the lowest effective degradabilities (EDs) of dry matter and crude protein. The crude protein (CP) ED values were ranked as follows: pumpkin seed cake > seed-used pumpkin flesh > pumpkin seed coat. Significant differences were observed in the peak areas of amide I and amide II and the corresponding peak heights in the two peak areas in the molecular structure of the protein. The peak areas of amide I and amide II and the corresponding peak heights were at the highest levels for pumpkin seed cake, whereas there was no significant difference between the pumpkin seed coat and seed-used pumpkin flesh. Similarly, the peak heights of α-helices and β-sheets were highest for pumpkin seed cake. Correlation and regression results indicated that amide I and amide II area and height, α-helix and β-sheet heights, and area ratios of amide I: amide II, as well as the height ratios of amide I: amide II, and α-helices: β-sheets effectively estimated nutrient supply and that the height ratio of α-helices: β-sheets was mostly sensitive to biodegradation characteristics in different byproducts of seed-used pumpkin. There were significant differences in CP chemical composition and digestibility of different byproducts of seed-used pumpkin that were strongly related to the changes in protein molecular structures.

An evaluation of the validity of an in vitro and an in situ/in vitro procedure for assessing protein digestibility of blood meal, feather meal and a rumen-protected lysine prototype.

In vitro procedures are commonly used to estimate rumen protein degradability and protein digestibility of feed ingredients. However, it is unclear how well these assays correlate to in vivo data. The objectives of this work were to compare postruminal protein availability estimates from one in vitro procedure and one in situ/in vitro procedure with in vivo observations for blood meal (BM), feather meal (FM), and a rumen-protected lysine prototype (RP-Lys). The FM and BM used for this experiment were subsamples of material assessed in vivo by an isotope-based method and the RP-Lys subsamples were of a prototype tested in two in vivo trials: a lactation trial and by plasma appearance. Subsamples of the BM (n = 14) and the FM (n = 22) were sent to each of three different laboratories for in vitro or in situ/in vitro analysis of crude protein (CP) and determination of rumen undegraded protein (RUP) and digested RUP (dRUP). Subsamples of the RP-Lys (n = 5) were sent to one laboratory for in vitro analysis of CP, RUP, and dRUP. Two diets containing BM or FM were assessed using the Cornell Net Carbohydrate and Protein System (CNCPS) v6.55 with ingredient inputs derived from either the CNCPS feed library, the isotope dilution method, or an average of the in vitro results from the three laboratories to determine how much the differences among estimates affected ingredient values. In vitro dRUP estimates for BM from one laboratory closely matched those determined in vivo (66.7% vs. 61.2%, respectively), but no in vitro estimates for FM matched the in vivo values. Surprisingly, there were significant differences in protein digestibility estimates from the modified three-step procedure across the two laboratories for BM (P < 0.0001) and for FM (P < 0.0001) indicating significant variation among laboratories in application of the method. Within all laboratories, BM estimates were reported in a narrow range (CV values of 2.6 or less). However, when testing multiple samples of FM or the RP-Lys prototype, CV values within a laboratory ranged up to 11 and 34, respectively. For the RP-Lys, dRUP estimates from the in vitro method were roughly half of that determined by the in vivo methods suggesting poor concordance between the in vitro and in vivo procedures for this ingredient. The inconsistencies within and among laboratories accompanied with dissimilarities to in vivo data is problematic for application in nutrition models. Additional refinement to the in vitro techniques is warranted.

Fermentation quality and protein fractions of four Pennisetum grass silages

AbstractIn order to make good use of Pennisetum grasses to relieve the shortage of animal feed, the nutrients, silage fermentation quality, and Cornell Net Carbohydrate and Protein System (CNCPS) protein fractions of Pennisetum spp. were evaluated in this study. Four Pennisetum cultivars of Reyan‐4, Mott, Guimu‐1, and MT‐1 were harvested at the first cut and second cut, and they were wilted and ensiled for the analyses of fermentation quality and protein fractions. The first‐cut grasses had higher crude protein (CP), water‐soluble carbohydrate (WSC) contents, and lower detergent fiber contents than the second cut ones. Mott had higher CP content. Reyan‐4 and MT‐1 contained higher WSC contents, and their silages had lower pH and ammonia nitrogen (NH3‐N) content, higher lactic acid content than Mott and Guimu‐1 silages. Wilting increased pH and NH3‐N contents and decreased lactic acid contents of both cut silages. The CNCPS analysis showed that fresh MT‐1 of either first or second cut had more evenly distributed protein fractions than other three grasses. Wilting and ensiling increased nonprotein nitrogen contents and decreased unavailable protein contents of four grasses. Four cultivars at the first cut contained more CP and less fiber than those at the second cut. Reyan‐4 and MT‐1 had better fermentation quality of silage than Mott and Guimu‐1; the latter might need additives to improve fermentation quality at ensiling.

Annual protein yield and extractable protein potentials in three legumes and two grasses.

New protein sources with low environmental and climatic impact are needed. Perennial crops show advantages as compared to annual crops and the upcoming biorefinery technology can extract proteins from the perennial biomass for protein concentrate production. The search for best-suited biomass crops needs to include harvest during the full growing season to support economic viability of biorefinery plants. Here we examined two grasses under increasing N fertilizer regime (175, 350 and 525 kg N ha-1 ) and three legumes, subject to a four-cut strategy. The well-defined Cornell Net Carbohydrate and Protein System (CNCPS) was used to estimate potential extractable protein. A key, previously presented in the literature, was applied in order to translate the CNCPS results into potential extracted protein concentrate. Crude protein (CP) yield per hectare was highest in red clover in 2015 (2907 kg CP ha-1 ) and the fertilized (525 kg N ha-1 ) tall fescue in 2016 (2435 kg CP ha-1 ). When translating the numbers into potential extraction of protein concentrate, the red clover had the highest protein concentrate yield per hectare in 2015 (835 kg CP ha-1 ) and lucerne in 2016 (803 kg CP ha-1 ). The results revealed that the entire season needs attention for optimization and not only the first cut, since both CP yields and quality peaks in different cuts across the five species and 2 years. Further knowledge of CP yield responses to field management and species mixtures are needed in order to advise farmers on the optimal crop for biorefining. © 2021 Society of Chemical Industry.

Lactational performance of dairy cows in response to supplementing N-acetyl-l-methionine as source of rumen-protected methionine

The objective of this experiment was to evaluate the effects of supplementing a rumen-protected source of Met, N-acetyl-l-methionine (NALM), on lactational performance and nitrogen metabolism in early- to mid-lactation dairy cows. Sixty multiparous Holstein dairy cows in early lactation (27 ± 4.3 d in milk, SD) were assigned to 4 treatments in a randomized complete block design. Cows were blocked by actual milk yield. Treatments were as follows: (1) no NALM (control); (2) 15 g/d of NALM (NALM15); (3) 30 g/d of NALM (NALM30); and (4) 45 g/d of NALM (NALM45). Diets were formulated using a Cornell Net Carbohydrate and Protein System (CNCPS) v.6.5 model software to meet or exceed nutritional requirements of lactating dairy cows producing 42 kg/d of milk and to undersupply metabolizable Met (control) or supply incremental amounts of NALM. The digestible Met (dMet) supply for control, NALM15, NALM30, and NALM45 were 54.7, 59.8, 64.7, and 72.2 g/d, respectively. The supply of dMet was 88, 94, 104, and 115% of dMet requirement for control, NALM15, NALM30, and NALM45, respectively. Milk yield data were collected, dry matter intake (DMI) was measured daily, and milk samples were collected twice per week for 22 wk. Blood, ruminal fluid, urine, and fecal samples were collected during the covariate period and during wk 4, 8, and 16. Data were analyzed using the GLIMMIX procedure of SAS (SAS Institute) using covariates in the model for all variables except body weight. Linear, quadratic, and cubic contrasts were also tested. Treatments did not affect DMI, milk yield, and milk component concentration and yield; however, feed efficiency expressed as milk yield per DMI and 3.5% fat-corrected milk per DMI were quadratically affected, with greater response observed for NALM15 and NALM30 compared with control. Acetate proportion linearly increased, whereas propionate proportion linearly decreased with NALM supplementation. Blood urea nitrogen linearly decreased with NALM supplementation. Total plasma essential AA concentrations were quadratically affected, as greater values were observed for control and NALM45 than other treatments. Plasma Met concentration was quadratically affected as lower levels were observed with NALM15, whereas Met concentrations increased with NALM45 compared with control. Nitrogen utilization efficiency and apparent total-tract nutrient digestibility were not affected by treatment. Supplementation of NALM at 15 or 30 g/head per day resulted in the greatest improvements in feed efficiency without affecting N metabolism of early- to mid-lactation dairy cows.

Evaluation of the nutritional value of faba beans with high and low tannin content for use as feed for ruminants.

Faba bean varieties with low or zero tannin content have been developed in Canada to overcome the negative effects of condensed tannins on the utilization by ruminants of crude protein (CP) and starch. However, their nutritional value has not been evaluated for incorporation in dairy rations. The objectives of this study were to investigate (i) the chemical profile; (ii) the Cornell Net Carbohydrate and Protein System (CNCPS) protein and carbohydrate subfractions; (iii) the energy values; (iv) the ruminal, intestinal, and total digestibility of CP; (v) the metabolizable protein (MP) supply to dairy cows; and (vi) the protein-inherent molecular spectral characteristics of brown-seeded (var. Fatima) faba beans with high tannin content and yellow-seeded (var. Snowbird) faba beans with low tannin content. The results revealed that Fatima beans had higher (P < 0.001) CP content than Snowbird (324 versus 295 g kg-1 dry matter (DM)), and lower (P < 0.01) starch content than Snowbird (411 g kg-1 DM versus 444 g kg-1 DM). Fatima had a lower (P=0.001) soluble subfraction (201 g kg-1 DM versus 220 g kg-1 DM) and higher (P < 0.05) slowly degradable fiber-bounded (24.9 g kg-1 DM versus 14.7 g kg-1 DM) and non-degradable (3.24 g kg-1 DM versus 0 g kg-1 DM) CNCPS CP subfractions than Snowbird. Fatima had higher (P=0.03) MP content (117 g kg-1 DM versus 111 gkg-1 DM) and metabolizable energy content (ME) 3.12 Mcal kg-1 versus 3.10 Mcal kg-1 ) than Snowbird. Molecular spectral intensities of amide I and II proteins (height and area) of Fatima were higher (P < 0.05) than those of Snowbird, reflecting their higher CP content. The ratio of protein spectral intensities, the amide I:amide II height ratio, and the α-helix:β-sheet height ratio differed (P < 0.05) between the two types of bean, highlighting differences in their inherent protein molecular structures. The (Fatima) faba beans with high condensed tannin content had higher MP and ME content. On average, both Faba beans had higher ME and MP content than barley grains, highlighting their promising nutritional value for dairy rations. © 2021 Society of Chemical Industry.