Core Populations Research Articles

Using stakeholder knowledge to co-produce research priorities for a raptor species vulnerable to impacts of wind energy facilities

The global energy transition necessitates a rapid increase in infrastructure in developing countries, including for wind energy facilities (WEFs). Concerns arise regarding the negative impacts of WEFs on biodiversity, especially on birds of prey or raptors and other volant animals. In Africa, South Africa has the largest installed wind energy capacity and also hosts several raptor species of conservation importance. One such raptor is the regionally endemic Jackal Buzzard Buteo rufofuscus (family Accipitridae), which has its core population in South Africa. Despite frequent fatalities from collisions with wind turbine blades, this species is understudied. To address this gap, we conducted interviews with key stakeholders in the wind energy sector, including avifaunal specialists, wind energy developers, government representatives, and conservation nongovernmental organisations. The goal was to begin a cooperative process to identify crucial research gaps and to establish a future research agenda for Jackal Buzzards in the context of wind energy development. Stakeholders expressed confidence in current environmental impact assessment (EIA) techniques but highlighted a concern for direct collisions as a significant threat to Jackal Buzzards. The consensus was that WEFs are more likely to cause local rather than national population-level impacts. Although stakeholders generally shared similar opinions, minor differences emerged between groups. Movement ecology studies were identified as a high priority for future research on this species. By collaboratively formulating research priorities, this study aims to improve the relevance of future investigations, fostering stakeholder buy-in for potential policy and EIA practice recommendations.

Does the Colonizing Population Exhibit a Reduced Genetic Diversity and Allele Surfing? A Case Study of the Midday Gerbil (Meriones meridianus Pallas) Expanding Its Range.

Colonizing populations at the leading edge of range expansion are expected to have a reduced genetic diversity and strong genetic structure caused by genetic drift and allele surfing. Until now, few studies have found the genetic signatures of allele surfing in expanding wild populations. Using mtDNA markers, we studied the genetic structure of the population of midday gerbils (Meriones meridianus) expanding their range to the west in Kalmykia (southern Russia) following the new cycle of desertification, re-colonizing areas abandoned in the mid-2010s. In the colonizing population, we found a reduced genetic diversity, the redistribution of haplotype frequencies-in particular, in favor of variants rare in the core population-and strong genetic structure combined with strong differentiation from the core population-patterns suggestive of allele surfing on the wave of expansion. In terms of genetic diversity and spatial structuration, the western edge population sampled in 2008 before its collapse in 2017 occupies the intermediate position between the current colonizing and core population. This suggests that reduced genetic diversity and increased genetic differentiation are general features of marginal populations, enhanced by the founder and allele-surfing effects at the leading edges of expanding ranges.

Evolution of ad hoc and routine data linkage in Australia

Australia has a rich history of utilising linked administrative data for health and human services research. The Population Health Research Network (PHRN), established in 2009, has developed national population-based data linkage capability and infrastructure. Australia-wide, specialist data linkage units have successfully linked their core population health data for over a decade, and within the network over 200 data collections are routinely linked. Data collections are typically classified as either routinely linked or linked on a project-by-project, ad hoc basis. Over time, linkage of data collections that were originally considered ad hoc, for example some longitudinal cohorts and clinical registries, has become ‘routine’. This paper reports on findings of a study that evaluated the volume and proportion of data linkage requests that have been facilitated through the PHRN over the past 5 years that include ad hoc linkage. The paper also reports on the type of data collections that have been linked on an ad hoc basis and details the type of research projects and fields of research currently supported via ad hoc linkage. Quantifying and characterising the type and most sought-after data collections for ad hoc linkage and the breadth of research being undertaken informs future planning and resource allocation for expanding the Australian national linkage capability. This paper explores the integration of ad hoc data linkage within the evolving landscape of expanded master linkage files and national linked data assets, responding to a growing range of data sources and research that could benefit from population-based data linkage.

Activated sludge microbial community assembly: the role of influent microbial community immigration.

Wastewater treatment plants (WWTPs) are host to diverse microbial communities and receive a constant influx of microbes from influent wastewater. However, the impact of immigrants on the structure and activities of the activated sludge (AS) microbial community remains unclear. To gain insight on this phenomenon known as perpetual community coalescence, the current study utilized controlled manipulative experiments that decoupled the influent wastewater composition from the microbial populations to reveal the fundamental mechanisms involved in immigration between sewers and AS-WWTP. The immigration dynamics of heterotrophs were analyzed by harvesting wastewater biomass solids from three different sewer systems and adding to synthetic wastewater. Immigrating influent populations were observed to contribute up to 14% of the sequencing reads in the AS. By modeling the net growth rate of taxa, it was revealed that immigrants primarily exhibited low or negative net growth rates. By developing a protocol to reproducibly grow AS-WWTP communities in the lab, we have laid down the foundational principles for the testing of operational factors creating community variations with low noise and appropriate replication. Understanding the processes that drive microbial community diversity and assembly is a key question in microbial ecology. In the future, this knowledge can be used to manipulate the structure of microbial communities and improve system performance in WWTPs.IMPORTANCEIn biological wastewater treatment processes, the microbial community composition is essential in the performance and stability of the system. This study developed a reproducible protocol to investigate the impact of influent immigration (or perpetual coalescence of the sewer and activated sludge communities) with appropriate reproducibility and controls, allowing intrinsic definitions of core and immigrant populations to be established. The method developed herein will allow sequential manipulative experiments to be performed to test specific hypothesis and optimize wastewater treatment processes to meet new treatment goals.

The genetic consequences of population marginality: A case study in maritime pine

AbstractAimMarginal tree populations, either those located at the edges of the species' range or in suboptimal environments, are often a valuable genetic resource for biological conservation. However, there is a lack of knowledge about the genetic consequences of population marginality, estimated across entire species' ranges. Our study addresses this gap by providing information about several genetic indicators and their variability in marginal and core populations identified using quantitative marginality indices.LocationSouthwestern Europe and North Africa.MethodsUsing 10,185 SNPs across 82 populations of maritime pine (Pinus pinaster Ait.), a widespread conifer characterised by a fragmented range, we modelled the relationship of seven genetic indicators potentially related to population evolutionary resilience, namely genetic diversity (based on both all SNPs and outlier SNPs), inbreeding, genetic differentiation, recessive genetic load and genomic offset, with population geographical, demo‐historical and ecological marginality (as estimated by nine quantitative indices). Models were constructed for both regional (introducing gene pool as a random factor) and range‐wide spatial scales.ResultsWe showed a trend towards decreasing overall genetic diversity and increasing differentiation with geographic marginality, supporting the centre‐periphery hypothesis (CPH). However, we found no correlation between population inbreeding and marginality, while geographically marginal populations had a lower recessive genetic load (only models without the gene pool effect). Ecologically marginal populations had a higher genomic offset, suggesting higher maladaptation to future climate, albeit some of these populations also had high genetic diversity for climate outliers.Main ConclusionsOverall genetic diversity (but not outlier‐based estimates) and differentiation patterns support the CPH. Ecologically marginal populations and those at the southern edge could be more vulnerable to climate change due to higher climate maladaptation, as predicted by genomic offsets, and/or lower potentially adaptive genetic diversity. This risk is exacerbated by typically small effective population sizes and increasing human impact in marginal populations.

High conservation importance of range-edge populations of Hooded Vultures (Necrosyrtes monachus)

Critically endangered Hooded Vultures (Necrosyrtes monachus Temminck, 1823), like many vulture species globally, are experiencing rapid population declines due to anthropogenic factors such as poisonings, human persecution, trading for belief-based use, and habitat loss/degradation. The Hooded Vulture is widespread across sub-Saharan Africa. Although it is considered one of the most abundant vultures in West Africa, this vulture species is less common in East and southern Africa, with the population at the southern-most edge of the distribution (in South Africa and Eswatini) estimated at only 100–200 mature individuals. The distribution of Hooded Vultures has contracted dramatically in southern Africa, with breeding populations largely confined to protected areas such as the Greater Kruger National Park. This study aimed to investigate the genetic diversity of the southern African range-edge population and assess if the recent contraction in the distribution has resulted in the population experiencing a genetic bottleneck. Sixteen microsatellite loci were amplified for samples collected along the Olifants River in the Greater Kruger National Park (n = 30). The genetic diversity in the South African population was compared to samples (n = 30) collected in Ghana, where Hooded Vultures are more abundant. Contrary to expectations, the South African peripheral Hooded Vulture population showed higher levels of heterozygosity (HO = 0.495) than the Ghanaian population (HO = 0.315). Neither population showed signs of recent bottleneck events when tested using demographic modelling and Approximate Bayesian computation (ABC). However, both populations showed high levels of inbreeding and relatedness. Our results suggest that despite being a small peripheral population, the South African Hooded Vulture population showed a similar level of genetic diversity as individuals sampled from a core population within the species distribution (in Ghana). This study supports the need for Hooded Vulture conservation efforts in the southern African region and highlights the evolutionary importance of range-edge populations.

Pushed waves, trailing edges, and extreme events: Eco-evolutionary dynamics of a geographic range shift in the owl limpet, Lottia gigantea.

As climatic variation re-shapes global biodiversity, understanding eco-evolutionary feedbacks during species range shifts is of increasing importance. Theory on range expansions distinguishes between two different forms: "pulled" and "pushed" waves. Pulled waves occur when the source of the expansion comes from low-density peripheral populations, while pushed waves occur when recruitment to the expanding edge is supplied by high-density populations closer to the species' core. How extreme events shape pushed/pulled wave expansion events, as well as trailing-edge declines/contractions, remains largely unexplored. We examined eco-evolutionary responses of a marine invertebrate (the owl limpet, Lottia gigantea) that increased in abundance during the 2014-2016 marine heatwaves near the poleward edge of its geographic range in the northeastern Pacific. We used whole-genome sequencing from 19 populations across >11 degrees of latitude to characterize genomic variation, gene flow, and demographic histories across the species' range. We estimated present-day dispersal potential and past climatic stability to identify how contemporary and historical seascape features shape genomic characteristics. Consistent with expectations of a pushed wave, we found little genomic differentiation between core and leading-edge populations, and higher genomic diversity at range edges. A large and well-mixed population in the northern edge of the species' range is likely a result of ocean current anomalies increasing larval settlement and high-dispersal potential across biogeographic boundaries. Trailing-edge populations have higher differentiation from core populations, possibly driven by local selection and limited gene flow, as well as high genomic diversity likely as a result of climatic stability during the Last Glacial Maximum. Our findings suggest that extreme events can drive poleward range expansions that carry the adaptive potential of core populations, while also cautioning that trailing-edge extirpations may threaten unique evolutionary variation. This work highlights the importance of understanding how both trailing and leading edges respond to global change and extreme events.

Comparative Study of the Kinetic Properties of Proton and Alpha Beams in the Alfvénic Wind Observed by SWA-PAS On Board Solar Orbiter

The problems of heating and acceleration of solar wind particles are of significant and enduring interest in astrophysics. The interactions between waves and particles are crucial in determining the distributions of proton and alpha particles, resulting in non-Maxwellian characteristics, including temperature anisotropies and particle beams. These processes can be better understood as long as the beam can be separated from the core for the two major components of the solar wind. We utilized an alternative numerical approach that leverages the clustering technique employed in machine learning to differentiate the primary populations within the velocity distribution rather than employing the conventional bi-Maxwellian fitting method. Separation of the core and beam revealed new features for protons and alphas. We estimated that the total temperature of the two beams was slightly higher than that of their respective cores, and the temperature anisotropy for the cores and beams was larger than 1. We concluded that the temperature ratio between alphas and protons largely over 4 is due to the presence of a massive alpha beam, which is approximately 50% of the alpha core. We provided evidence that the alpha core and beam populations are sensitive to Alfvénic fluctuations and the surfing effect found in the literature can be recovered only when considering the core and beam as a single population. Several similarities between proton and alpha beams would suggest a common and local generation mechanism not shared with the alpha core, which may not have necessarily been accelerated and heated locally.

Core to ultracompact HII region evolution in the W49A massive protocluster

Aims. We aim to identify and characterize cores in the high-mass protocluster W49A, determine their evolutionary stages, and measure the associated lifetimes. Methods. We built a catalog of 129 cores extracted from an ALMA 1.3 mm continuum image at 0.26″ (2900 au) angular resolution. The association between cores and hypercompact or ultracompact HII (H/UC HII) regions was established from the analysis of VLA 3.3 cm continuum and H30α line observations. We also looked for emission of hot molecular cores (HMCs) using the methyl formate doublet at 218.29 GHz. Results. We identified 40 cores associated with an H/UC HII region and 19 HMCs over the ALMA mosaic. The 52 cores with an H/UC HII region and/or an HMC are assumed to be high-mass protostellar cores, while the rest of the core population likely consists of prestellar cores and low-mass protostellar cores. We found a good agreement between the two tracers of ionized gas, with 23 common detections and only four cores detected at 3.3 cm and not in H30α. The spectral indexes from 3.3 cm to 1.3 mm range from 1, for the youngest cores with partially optically thick free-free emission, to about −0.1, which is for the optically thin free-free emission obtained for cores that are likely more evolved. Conclusions. Using the H/UC HII regions as a reference, we found the statistical lifetimes of the HMC and massive protostellar phases in W49N to be about 6 × 104 yr and 1.4 × 105 yr, respectively. We also showed that HMCs can coexist with H/UC HII regions during a short fraction of the core lifetime, about 2 × 104 yr. This indicates a rapid dispersal of the inner molecule envelope once the HC HII is formed.

An ALMA Search for Substructure and Fragmentation in Starless Cores in Orion B North

We present Atacama Large Millimeter/submillimeter Array (ALMA) Cycle 3 observations of 73 starless and protostellar cores in the Orion B North molecular cloud. We detect a total of 34 continuum sources at 106 GHz, and after comparisons with other data, four of these sources appear to be starless. Three of the four sources are located near groupings of protostellar sources, while one source is an isolated detection. We use synthetic observations of a simulation modeling a collapsing turbulent, magnetized core to compute the expected number of starless cores that should be detectable with our ALMA observations and find at least two (1.52) starless cores should be detectable (at 5σ), consistent with our data. We run a simple virial analysis of the cores to put the Orion B North observations into context with similar previous ALMA surveys of cores in Chamaeleon I and Ophiuchus. We conclude that the Chamaeleon I starless core population is characteristically less bounded than the other two populations, along with external pressure contributions dominating the binding energy of the cores. These differences may explain why the Chamaeleon I cores do not follow turbulent model predictions, while the Ophiuchus and Orion B North cores are consistent with the model.

Pond area and availability safeguard amphibian genetic diversity across Iberia's largest protected wetland

Abstract Wetland ecosystems worldwide are threatened by habitat alteration, climate change and the introduction of invasive species, even within protected areas. Unravelling the reliance of sensitive wetland‐dwelling species, such as amphibians, on habitat characteristics is thus essential to identify conservation targets. Here we assess the distribution of genetic diversity of two strongly aquatic amphibians (Pelophylax perezi and Pleurodeles waltl) in association with habitat features across the most extensive, protected wetland of the Iberian Peninsula: Doñana National Park. Despite inhabiting a protected area free from anthropogenic barriers, the genetic diversity of P. perezi and P. waltl is not homogeneously distributed across the wetland, but instead concentrates in core areas, mainly in the northern zone. Both genetic diversity and connectivity (as opposed to genetic differentiation) showed significant positive associations with the area of the breeding sites and the flooded area surrounding the breeding sites within the dispersal potential of either species, that is nearby pond availability. Large water bodies connected to abundant temporary ponds are key for the maintenance of amphibian genetic diversity. Nevertheless, the core populations of our target species, which show markedly aquatic habits, are concentrated in areas colonised by invasive species, which could compromise their long‐term viability. Our results highlight that maintaining widely connected arrays of ponds of different hydroperiods, including large breeding sites free from invasive predators and competitors, is paramount for amphibian conservation in Mediterranean wetlands.

Massive clumps in W43-main: Structure formation in an extensively shocked molecular cloud

Aims. W43-main is a massive molecular complex undergoing starburst activities, located at the interaction of the Scutum arm and the Galactic bar. We aim to investigate the gas dynamics, in particular, the prevailing shock signatures from cloud to clump scales. We also look to assess the impact of shocks on the formation of dense gas and early-stage cores in OB cluster formation processes. Methods. We carried out NOEMA and IRAM-30 m observations at 3 mm towards five molecular gas clumps in W43 main located within large-scale interacting gas components. We used CH3CCH and H2CS lines to trace the extended gas temperature and CH3OH lines to probe the volume density of the dense gas components (≳105 cm−3). We adopted multiple tracers that are sensitive to different gas density regimes to reflect the global gas motions. The density enhancements constrained by CH3OH and a population of NH2D cores are correlated (in the spatial and velocity domains) with SiO emission, which is a prominent indicator of shock processing in molecular clouds. Results. The emission of SiO (2–1) is extensive across the region (~4 pc) and it is contained within a low-velocity regime, hinting at a large-scale origin for the shocks. Position-velocity maps of multiple tracers show systematic spatio-kinematic offsets supporting the cloud-cloud collision-merging scenario. We identified an additional extended velocity component in the CCH emission, which coincides with one of the velocity components of the larger scale 13CO (2−1) emission, likely representing an outer, less-dense gas layer in the cloud merging process. We find that the ‘V-shaped’, asymmetric SiO wings are tightly correlated with localised gas density enhancements, which is direct evidence of dense gas formation and accumulation in shocks. The dense gas that is formed in this way may facilitate the accretion of the embedded, massive pre-stellar and protostellar cores. We resolved two categories of NH2D cores: those exhibiting only subsonic to transonic velocity dispersions and those with an additional supersonic velocity dispersion. The centroid velocities of the latter cores are correlated with the shock front seen via SiO. The kinematics of the ~0.1 pc NH2D cores are heavily imprinted by shock activities and may represent a population of early-stage cores forming around the shock interface.

Assessing microbial stability and predicting biogas production in full-scale thermophilic dry methane fermentation of municipal solid waste

Compared to typical anaerobic digestion processes, little is known about both sludge microbial compositions and biogas production models for full-scale dry methane fermentation treating municipal solid waste (MSW). The anaerobic sludge composed of one major hydrogenotrophic methanogen (Methanoculleus) and syntrophic acetate oxidizing bacteria (e.g., Caldicoprobacter), besides enrichment of MSW degraders such as Clostridia. The core population remained phylogenetically unchanged during the fermentation process, regardless of amounts of MSW supplied (∼35 ton/d) or biogas produced (∼12000 Nm3/d). Based on the correlations observed between feed amounts of MSW from 6 days in advance to the current day and biogas output (the strongest correlation: r = 0.77), the best multiple linear regression (MLR) model incorporating the temperature factor was developed with a good prediction for validation data (R2 = 0.975). The proposed simple MLR method with only data on the feedstock amounts will help decision-making processes to prevent low-efficient biogas production.

A computational- experimental model of reactor kinetic for investigating the linearity response of in-core neutron detectors of a low power research reactor

In this study, the linear response of fission chamber (FC) neutron detectors in Isfahan miniature neutron source reactor (MNSR) is investigated through experiments and calculations at different powers of the reactor. The linearity of the response of the main neutron detector in the MNSR is important due to its safety functions in reactor power control. Additionally, determining the linear response region of this detector presents a linear neutron dosimetry field even outside of the reactor core, i.e. at the outlet of external beam tubes. Moreover, it is very difficult to remove and calibrate this neutron detector that has been installed in the reactor core for a long time. For this purpose, an alternative method for measuring the linearity of the response of a neutron detector is extended by investigating the kinetic behavior of reactor. The aim of this method is to calculate the delayed neutrons population in the reactor core and compare with the FC detector response. In this method, the numerical solution method is used to solve the point kinetic equation with 15 delayed neutron groups (6 delayed neutron groups and 9 photo-neutron groups) after inserting a large negative reactivity into the MNSR core by considering temperature and xenon reactivity feedbacks during a coupling procedure. To obtain the xenon reactivity feedback, xenon concentration is calculated by coupling the 135Xe and 135I production equations and the εpPFnlPTnl parameter is obtained by an experimental method. Furthermore, the value of fission cross section (Σf) is calculated using a validated model in the Dragon code. The large reactivities are inserted at two initial power of 150 W and 30 kW to investigate the response of the main FC neutron detector in a cold xenon-free condition and by considering temperature and xenon reactivity feedbacks, respectively. The results demonstrate that the response of the MNSR FC detector is linear in the neutron flux range between 5 × 107 to 1 × 1012 n.cm−2.s−1, which is equivalent to reactor power of 1.5 W to 30 kW. This innovative method can be applied to calibrate the neutron detectors in other research and power reactors.

Abstract 2155: The impact of urban residence on symptom duration, treatment initiation, and survival among primary brain tumor patients: A large cohort analysis

Abstract Primary brain tumors (PBT) are rare, accounting for less than 2% of all cancers, present acutely, and often require specialized care to determine treatment approaches. Neuro-Oncology clinics are located in densely populated urban areas resulting in additional access barriers, such as longer travel time, for those in non-urban areas. To better understand how proximity to our facility affects patient outcomes among PBT patients enrolled in the National Institutes of Health (NIH) Natural History Study (NCT02851706, PI: Armstrong), we assessed the association of population density with symptom duration before diagnosis, time to first treatment, and overall survival among a cohort of 666 adult PBT patients. More urbanized areas were defined as areas with a core population density of more than 1,000 persons per square mile. T-tests and chi-squared tests were performed to assess differences in patient and tumor characteristics based on population density (more urbanized vs. less urbanized). We used ordinal logistic regression (odds ratio [OR], 95% confidence intervals [CI]) to assess the association between duration of presenting symptoms (<6 months, 6 months-1 year, ≥1 year) and linear regression (beta coefficient, 95% CI) for time to treatment and population density. We adjusted for age at cancer diagnosis and sex (male, female), and results were stratified according to residential distance to the NIH (short distance=<200 miles, long distance=200+ miles), to control for local referral patterns. Kaplan-Meier estimates were used to assess overall survival by population density levels, overall, and by residential distance. All models were then further stratified by tumor grade (low, high). Among 666 patients diagnosed with PBTs, 401 (60%) lived in more urbanized areas, 40% were long distance, and 24% had low-grade tumors. Patients living in more urbanized areas were more racially diverse (p<0.001), had higher educational attainment (p<0.001), and were more likely to be single (p=0.008). In the overall cohort and when stratified by distance to NIH or tumor grade, there were no associations between population density level and symptom duration prior to diagnosis. Time to treatment for radiation and chemotherapy was longer for PBT patients living in less urbanized areas and longer distances from the NIH (β=0.63; 95%CI=0.08,1.17) compared to those living in more urbanized areas. There were no differences in the 5-year survival rates between more or less urbanized groups. In conclusion, access to care for those in less urbanized areas was demonstrated, although survival was not impacted. Future consideration should be given to novel approaches to increase access to specialized care, particularly for PBT patients living further from urban areas. Citation Format: Macy L. Stockdill, Jacqueline B. Vo, Orieta Celiku, Zuena Karim, Yeonju Kim, Hope Miller, Elizabeth Vera, Terri S. Armstrong. The impact of urban residence on symptom duration, treatment initiation, and survival among primary brain tumor patients: A large cohort analysis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 2155.

Tourist Trampling on a Peripheral Plant Population Restricted to an Urban Natural Area in the Capo Sant’Elia Promontory (Sardinia, W-Mediterranean Basin)

Urban natural areas provide important ecological services such as biodiversity conservation, as well as opportunities for people to connect with nature and preserve cultural heritage. However, the increasing demand for access to natural areas and the expansion of human recreational activities, such as hiking and biking, pose threats to these ecosystems, especially for animal and plant species, finally resulting in biodiversity loss. This study explores the intricate link between human trampling, plant density, and the morphological and reproductive characteristics of Globularia alypum L., a plant with a peripheral population in Sardinia restricted to a natural area within an urban context. The study examined trampling effects on 75 plots along a frequently used path crossing the plant’s core population. Similar environmental conditions were assumed, with differences attributed to human trampling intensity and plant density, and morphological and reproductive traits were measured within each plot. Our results showed that human trampling caused differences in the morphological traits of plants, whereas, in contrast, reproductive traits are less vulnerable to human trampling than morphological ones. As a result, trampled areas may experience decreased plant recruitment, which can have long-term implications for plant population dynamics. Understanding the relationship between trampling effects and the sensitivity of peripheral plant populations is crucial for effective conservation and management strategies.

Evaluation of Current Surgical BPH Interventions for Young and Elderly Men.

Benign prostatic hyperplasia affects the quality of life of a significant number of men, especially as they age. There are continuous innovations in the surgical management of benign prostatic hyperplasia, but many of these innovations are studied in the core population of men 50-70years of age. This review focuses on the outliers of men aged 18-50 and 70 and older. Older populations have more comorbidities, higher rates of antithrombotic medications, and advanced symptoms. Properly selected older men can safely have significant objective and subjective improvement in their symptoms. The literature was scarce when evaluating younger men; however, ejaculatory preserving techniques are promising providing improvement in symptoms and preserving ejaculation. This review demonstrates that in properly selected elderly patients, improvements in quality of life while also providing safe surgical interventions can be achieved. Ejaculatory preservation techniques demonstrate promising results, but further studies are required to elucidate true outcomes.

Distribution, density and trends of the Eurasian eagle owl (Bubo bubo) population breeding in March-Thaya floodplain forests: impact of owlets on sustainability of natural nests

Abstract In this study, we investigated the Eurasian eagle owl (Bubo bubo) breeding in lowland forests in the trans-border area between western Slovakia, eastern Austria, and southern Moravia. The research provides new information on the reuse of nests by eagle owls and presents initial insights into population density and trends of eagle owls in the March-Thaya floodplains. Our 19-year monitoring has shown that the eagle owl has become a widespread breeder over the study area, with an increasing population trend. A total of 151 breeding attempts by eagle owls have been identified, occupying 82 natural nests (originally built by at least nine species of birds) and 12 artificial nests. With an average of 6.2 active nests per 100 km2 and a maximum of 17 active nests found in 2021 (~10.6 pairs per 100 km2), our findings represent one of the highest eagle owl breeding densities found, especially in comparison with core populations nesting in the mountains (the Carpathians, north-eastern Alps and the Bohemian Massif). Regarding the dynamics of nest reuse, our results reveal that only a third of nests used by eagle owls were reused by other raptors or storks (Ciconia sp.). Almost 50% of the natural nests in which eagle owl bred, subsequently disintegrated after the owlets had fledged. Lastly, black stork (Ciconia nigra) nests re-used by eagle owls were twice as likely to have disintegrated after the owlets had fledged than nests built by other bird species. Our results suggest that black stork nests in the March and Thaya floodplain forests are most susceptible to destruction.

Home ranges and hatching success of threatened Eurasian curlew in north-eastern Europe relates to habitat type: Natural vs. agricultural landscapes

Farmland birds are among the most threatened species in Europe, largely as a result of the intensification of agriculture leading to widespread biodiversity losses. Ground-breeding shorebirds in grassland and arable habitats are particularly exposed to human threats and predation in highly modified landscapes; however, parts of their populations still breed in natural habitats and could provide a reservoir for threatened populations. This study aimed to improve our knowledge of the spatio-temporal use of breeding habitats by Eurasian curlew Numenius arquata arquata in a core area of the population in remote north-eastern Europe. A total of 83 adult birds across Europe were tagged with GPS devices between 2014 and 2022 to analyse their home-range sizes, habitat use and hatching success. Birds were distributed at breeding sites from 52°N to 66°N between Finland and the Ural Mountains. Forty-one percent of individuals nested in bogs, 33% in grassland, and 20% in abandoned farmland. Birds nested predominantly in bogs at northern latitudes and in grassland at southern latitudes, while abandoned farmland was mainly used at intermediate latitudes. The mean home range was largest for birds breeding predominantly in grassland while birds in bogs had the highest hatching success rate. Russia and Finland host the core population of the subspecies N. a. arquata during breeding period, which is essential for preserving the species on a European scale. Their survival may depend on well-preserved bogs and the maintenance of grassland areas, given that large areas of abandoned farmland are destined to become either forest or to be reclaimed for cereal crops or fodder grass in the short and medium terms. Given the large breeding populations in both countries, Russia and Finland have major responsibilities to maintain these essential populations on a biogeographical scale.

Real-World Effectiveness and Safety of Glecaprevir/Pibrentasvir for the Treatment of Chronic Hepatitis C: A Prospective Cohort Study in Portugal.

Information about pan-genotypic treatments for hepatitis in Portugal is scarce. We aimed to evaluate the effectiveness and safety of glecaprevir plus pibrentasvir (GLE/PIB) treatment for hepatitis C virus (HCV) infection in real-world clinical practice. An observational prospective study was implemented in six hospitals with 121 adult HCV patients who initiated treatment with GLE/PIB between October 2018 and April 2019, according to clinical practice. Eligible patients had confirmed HCV infection genotype (GT) 1 to 6 and were either treatment-naïve or had experience with interferon-, ribavirin- or sofosbuvir-based regimens, with or without compensated cirrhosis. Baseline sociodemographic and safety data are described for the total population (N = 115). Effectiveness [sustained virologic response 12 weeks after treatment (SVR12)] and patient-reported outcomes are presented for the core population with sufficient follow-up data (n = 97). Most patients were male (83.5%), aged < 65 years (94.8%), with current or former alcohol consumption (77.3%), illicit drug use (72.6%), and HCV acquisition through intravenous drug use (62.0%). HIV co-infection occurred in 22.6% of patients. The prevalence of each GT was: GT1 51.3%, GT2 1.7%, GT3 30.4%, GT4 16.5%, and GT5.6 0%. Most patients were non-cirrhotic (80.9%) and treatment-naïve (93.8%). The SVR12 rates were 97.9% (95% CI: 92.8 - 99.4), and > 95% across cirrhosis status, GT, illicit drug use, alcohol consumption, and HCV treatment experience. The adverse event rate was 2.6%, and no patient discontinued treatment due to adverse events related to GLE/PIB. Consistent with other real-world studies and clinical trials, treatment with GLE/PIB showed high effectiveness and tolerability overall and in difficult-to-treat subgroups (ClinicalTrials.gov: NCT03303599).