Using humor as a coping tool in the face of stressful situations increases positive affect and well-being by enabling individuals to evaluate the negative situation from a new perspective. In this study, the mediating role of reappraisal in the relationship between coping through humor and mental well-being was examined. The sample of the study consisted of 505 university students studying at state universities in Turkey in the 2023-2024 academic year. Data were collected from students in state universities in 20 different cities of Turkey. Considering the ease of accessing data, the convenient sampling method was preferred. The Coping with the Humor Scale, the Warwick-Edinburgh Mental Well-Being Scale Short Form, and the Reappraisal subscale of the Emotion Regulation Scale were used as data collection tools in the study. The reliability of the scales used in the study was examined by confirmatory factor analysis. The relationships between variables were examined by Pearson correlation analysis and regression analysis was performed using Process Macro (model 4). In the regression analysis, mediation was tested with the Bootstrap technique. According to the results of correlation and regression analyses, it was seen that there were significant positive relationships between coping through humor, mental well-being, and reappraisal. According to the results of regression-based Bootstrap analysis, coping through humor is a predictor of mental well-being and reappraisal has a partial mediating role in the relationship between coping through humor and mental well-being. It is thought that the results obtained can contribute to research and application examples involving humor and reappraisal in increasing the mental well-being of university students and fill a gap in the literature.