Copigmented Anthocyanins Research Articles

Effect of maltodextrin and Persian gum as wall materials and tannic acid as copigment on some properties of encapsulated sour cherry anthocyanin microcapsules

Due to the instability of anthocyanins, their application as natural colorants is limited. To improve their stability, anthocyanins extracted from sour cherry were copigmented with tannic acid at varying molar ratios. The optimal anthocyanin:copigment molar ratio was determined to be 1:0.25. Subsequently, both non-copigmented and copigmented anthocyanins (using the optimal tannic acid molarity) were spray-dried with either maltodextrin alone (T1 and T2) or a combination of maltodextrin and Persian gum (T3 and T4). The anthocyanin retention in T2 and T4 was approximately 53 % and 38 %, respectively, which were higher than in the non-copigmented samples. All powders demonstrated high encapsulation efficiency (>90.37 %). Stability tests on the anthocyanins conducted over 28 days indicated that light exposure had no effect on the reduction of anthocyanin content when maltodextrin was used. Thus, the copigmentation of anthocyanins with tannic acid, combined with encapsulation in maltodextrin, presents a promising method for producing a stable natural colorant.

A new enzymatic clarification method for pomegranate juice: Removal of defects and improvement of quality by tannase, lactonase and papain

Effects of clarification with “tannase,” “tannase+lactonase,” “papain,” “aspartic protease” and “tannase+lactonase+papain” on defects (turbidity, sediment and color loss) and important constituents (phenolics, anthocyanins, proteins and amino acids) of pomegranate juice (PJ) were investigated. Lactonase [human-derived (H-PON-1 and H-PON-2) and rabbit-derived (R-PON-1, R-PON-2 and R-PON-3)] was used during juice clarification for the first time. Phenolics and amino acids were quantified by HPLC-DAD-MS and HPLC. After clarifications with “tannase (1 g/L)+R-PON-1 (0.33 mg/L)” and “tannase (1 g/L)+R-PON-1 (0.33 mg/L)+papain (10 mg/L)” at 30℃ for 90 min, sediment was completely eliminated during storage at 20℃ for a month. Among these treatments, “tannase+R-PON-1” provided the lowest turbidity formation rate. The most effective parameter to prevent sediment was the ratio of “prolamin to trigalloyl-HHDP-glucose-isomer-2” that should be ≥205.4 (r=–1.000). Moreover, “tannase+R-PON-1” caused 4.3 times lower turbidity than control group after storage. To retain low turbidity (≤10 NTU) during storage, galloyl-glucose-isomer and HHDP-galloyl-hexoside-3 should completely be removed from PJ and, punigluconin and trigalloyl-HHDP-glucose-isomer-2 contents should be ≤0.19 and ≤0.04 mg/L, respectively. Furthermore, “tannase+R-PON-1” provided a significant contribution to anthocyanin copigmentation. The most effective copigments in PJ were HHDP-galloyl-glucuronide, punigluconin, punicalagin-2, punicalagin-3, aspartic acid, glutamic acid and alanine. “Tannase+R-PON-1” is recommended to produce a high-quality PJ.

Intermolecular copigmentation of anthocyanins with phenolic compounds improves color stability in the model and real blueberry fermented beverage

To improve the color stability of anthocyanins (ACNs) in blueberry fermented beverage, the intermolecular copigmentation between ACNs and 3 different phenolic compounds, including (−)-epigallocatechin gallate (EGCG), ferulic acid (FA), and gallic acid (GA) as copigments, was compared in the model and the real blueberry fermented beverage, respectively. The copigmented ACNs by EGCG presented a high absorbance (0.34 a.u.) and redness (27.09 ± 0.17) in the model blueberry fermented beverage. The copigmentation by the participation of the 3 different phenolic compounds showed all a spontaneous exothermic reaction, and the Gibbs free energy (ΔG°) of the system was lowest (−5.90 kJ/mol) using EGCG as copigment. Furthermore, the molecular docking model verified that binary complexes formed between ACNs and copigments by hydrogen bonds and π-π stacking. There was a high absorbance (1.02 a.u.), percentage polymeric color (PC%, 68.3 %), and good color saturation (C*ab, 43.28) in the real blueberry fermented beverage aged for 90 days, and more malvidin-3-O-glucoside had been preserved in the wine using EGCG as copigment. This finding may guide future industrial production of blueberry fermented beverage with improved color.

Differences in the Behavior of Anthocyanin Coloration in Wines Made from Vitis vinifera and Non-vinifera Grapes

The skins of Vitis vinifera species contain 3-glucosyl anthocyanins (3G), but some non-vinifera species, such as ‘Yama Sauvignon’ (YS), contain a large amount of 3,5-diglucosyl anthocyanins (35DG), and the behavior of anthocyanin coloration with respect to pH is quite different. The anthocyanins of YS showed a very weak color at a pH of 3 or higher but a very strong color below a pH of 3. Furthermore, when we investigated the effect of co-pigmentation in commercially available wines, we found that YS red wine contained a large amount of co-pigmented anthocyanins, and even wine aged for about 4 years contained a large amount of co-pigmented anthocyanins. Due to concerns regarding disease resistance, many hybrid varieties of V. vinifera and non-vinifera species have been bred, but it is important to take these special properties of 35DG into consideration when producing wine.

Effect of exogenous organic acids on chemical compositions and sensory attributes of fortified sweet wines from dehydrated grapes

In this study, acidity was regulated with the addition of exogenous tartaric acid and citric acid before bottling. The effect of exogenous organic acids on chemical compositions and sensory attributes of fortified sweet wines from dehydrated grapes were investigated. The results indicated that exogenous organic acids promoted the conversion of monomeric anthocyanins to copigmented anthocyanins in wines. Specifically, the combination of malvidin-3-O-glucoside and flavanols (catechin and epicatechin) was facilitated to form copigmented anthocyanins. Sensory analysis suggested that exogenous organic acids improved the balance of sugar and acidity and benefited the harmony in wines on the taste. Wines with a residual sugar and titratable acidity ratio of about 11:1 exhibited the more harmonious taste. In addition, it was also observed changes in the aroma profile related to volatile compounds, namely, more intense fruity aroma in wines with the addition of organic acids.

Co-pigmentation of Pinotage anthocyanins with Cyclopia (honeybush) polyphenols in model wine solutions

Co-pigmentation of an anthocyanin-enriched fraction (AEF) of Pinotage wine with honeybush polyphenols (Cyclopia subternata extract and fractions enriched in specific polyphenols) was studied in model wine solutions. The hyperchromic effects and bathochromic shifts depended on the co-pigment sources and co-pigment/anthocyanin ratio. Fraction 2 (F2), enriched in xanthones (mangiferin and isomangiferin), produced the most pronounced co-pigmentation effect at a co-pigment/anthocyanin ratio of 15:1. Greater co-pigmentation effects were observed in model solutions at pH 3.0 (pH 3–4 evaluated) and in the absence of EtOH (0–16 % EtOH evaluated). During storage of solutions (AEF and/or F2) at 15 °C and 25 °C for 12 weeks, myrtillin, petunin, 3′,5′-di-β-D-glucopyranosyl-3-hydroxyphloretin and mangiferin concentrations decreased following first-order kinetics, while peonin and oenin degradation followed fractional-conversion kinetics. Polymeric anthocyanin formation followed zero-order kinetics. Reaction rates increased with an increase in storage temperature. The addition of F2 to the AEF solution produced a darker, more vivid colour and improved the colour stability, however, the yellow-orange colour of F2 and intensification of its colour during storage contributed to increasing red-orange hues. Further research is necessary to elucidate the effect of honeybush polyphenols on the colour and composition of a complete wine as it is more complex than model solutions.

Stabilization of anthocyanins by simultaneous encapsulation-copigmentation via protein-polysaccharide polyelectrolyte complexes.

This study prepared a series of polyelectrolyte complexes (PECs) composed of heated whey protein isolate (HWPI) and different polysaccharides for simultaneous encapsulation and copigmentation of anthocyanins (ATC) and their ultimate stabilization. Four polysaccharides including chondroitin sulfate, dextran sulfate, gum arabic, and pectin were chosen due to their abilities to simultaneously complex with HWPI and copigment ATC. At pH 4.0, these PECs were formed with an average particle size of 120-360nm, the ATC encapsulation efficiency of 62-80%, and the production yield of 47-68%, depending on the type of polysaccharides. The PECs effectively inhibited the degradation of ATC during storage and when exposed to neutral pH, ascorbic acid, and heat. Pectin had the best protection, followed by gum arabic, chondroitin sulfate, and dextran sulfate. The stabilizing effects were associated with the hydrogen bonding, hydrophobic and electrostatic interactions between HWPI and polysaccharides, conferring dense internal network and hydrophobic microenvironment in the complexes.

Forcing vine regrowth under different irrigation strategies: effect on polyphenolic composition and chromatic characteristics of cv. Tempranillo wines grown in a semiarid climate.

One of the effects of climate change in warm areas is the asynchrony between the dates of the technological and the phenolic maturity of grapes. This is important because the quality and color stability of red wines are directly related to the content and distribution of phenolic compounds. A novel alternative that has been proposed to delay grape ripening and make it coincide with a seasonal period more favorable for the formation of phenolic compounds is crop forcing. This consists of severe green pruning after flowering, when the buds of the following year have already differentiated. In this way, the buds formed during the same season are forced to sprout, initiating a new delayed cycle. The aim of the present work is to study the effect on the phenolic composition and color of wines elaborated from vines fully irrigated (C), grown using conventional non-forcing (NF) and forcing (F) techniques (C-NF and C-F), and wines from vines subjected to regulated irrigation (RI), grown using NF and F techniques (RI-NF and RI-F). The trial was carried out in an experimental vineyard of the Tempranillo variety located in a semi-arid area (Badajoz, Spain) in the 2017-2019 seasons. The wines (four by treatment) were elaborated and stabilized according to the classic methodologies for red wine. All wines had the same alcohol content, and malolactic fermentation was not carried out in any of them. Anthocyanin profiles were analyzed by HPLC, and total polyphenolic content, anthocyanin content, catechin content, the contribution to color due to co-pigmented anthocyanins, and various chromatic parameters were also determined. Although a significant effect of year was found for almost all the parameters analyzed, a general increasing trend in F wines was found for most of them. The anthocyanin profile of F wines was found to differ from that of C wines, especially in delphinidin, cyanidin, petunidin, and peonidin content. These results indicate that by using the forcing technique it was possible to increase the polyphenolic content by ensuring that the synthesis and accumulation of these substances occurred at more suitable temperatures.

Lipote fruit anthocyanin-rich extracts as food colourants: extraction optimization and stability of natural and copigmented anthocyanins

Natural pigments such as anthocyanins are currently being explored for food colouring applications due to safety issues associated with synthetic colourants. Lipote (Syzygium polycephaloides), an underutilized fruit in the Philippines, is found to contain high amounts of this natural pigment. Thus, this study aimed to optimize parameters for maximum anthocyanin extraction from these fruits by mixture design and response surface methodology followed by the evaluation of the extracts’ stability using model beverage systems. Results revealed that the optimum solvent for maximum anthocyanin recovery was 55% acidified ethanol while optimum extraction temperature and time were 45.8°C and 92 mins, respectively. Stability tests showed that the lipote fruit extract, in its natural form, was most stable in the dark, at low pH (pH 3.0) and low storage conditions (4°C) with an extrapolated half-life (t1/2) of 169.06 days. Anthocyanin stability was further enhanced by copigmentation with caffeic acid resulting in an even higher t½ of 223.60 days.

A novel anthocyanin electrospun film by caffeic acid co-pigmentation for real-time fish freshness monitoring.

In recent years, the use of intelligent and efficient food freshness indicators (FFIs) for monitoring food freshness has been studied widely. In this work, we employed polyacrylonitrile as polymer, blueberry anthocyanins as an indicator, and caffeic acid as a co-pigment and fabricated a novel colorimetric sensing film for real-time monitoring the freshness of fish. The total volatile basic nitrogen (TVB-N) level is one of the potential indicators to evaluate meat freshness. Visual observation confirmed that the polyacrylonitrile-anthocyanin-caffeic acid film changed from pink to light purple, and then to dark purple providing a good indication of spoilage, which correlated well with the TVB-N content and pH values in fish. It is because the volatile ammonia combined with water to form NH3·H2O, and then NH3·H2O is hydrolyzed to form OH- and NH4+. The change of the polyacrylonitrile-anthocyanin film was caused by OH-. Compared with the polyacrylonitrile-anthocyanin film without caffeic acid, the addition of the caffeic acid film had enhanced significantly ammonia responsiveness with a total color difference value of 29.897. And it was also observed that caffeic acid obviously improved the storage stability of the film. This study provided a reference for detecting food freshness using co-pigmentation and electrospinning encapsulation technology in combination.

A Case Study for the Extraction, Purification, and Co-Pigmentation of Anthocyanins from Aronia melanocarpa Juice Pomace.

Chokeberry (Aronia melanocarpa) pomace is a by-product from the juice industry very rich in anthocyanins and other bioactive components. Recovery and purification of anthocyanins from the pomace is a viable valorization strategy that can be implemented to produce high-value natural food colorants with antioxidant properties. In this study, chokeberry pomace was subjected to enzyme-assisted extraction using commercial pectinases. The extracts were further purified by adsorption-desorption using an acrylic resin and stabilized by co-pigmentation with ferulic acid. The anthocyanin concentration and antioxidant activity of the extracts were unaffected by the enzymatic treatment at the conditions tested. The total phenolic content of the extracts suffered minor variations depending on the enzyme formulation used, whereas the dissolved solid content increased in all cases. The adsorption-desorption strategy allowed a 96% recovery of the anthocyanins initially present in the extract, whereas the co-pigmentation treatment magnified the intensity of the color in terms of absorbance, and improved the stability during storage up to one month.

Co-Pigmentation Mechanism and Thermal Reaction Kinetics of Mulberry Anthocyanins with Different Phenolic Acids.

Applying the intermolecular co-pigmentation to improve the stability of mulberry anthocyanins is an important co-pigment method. Seven co-pigments, ferulic acid (FA), caffeic acid (CA), p-hydroxybenzoic acid (HBA), protocatechuic acid (PA), gallic acid (GA), vanillic acid (VA) and vanillin (VN) were selected to investigate mulberry anthocyanin co-pigmentation thermal reaction kinetics. The strongest co-pigment reactions were observed for FA at a molar ratio of 1:20, pH 3.5 and 20 °C, with the highest hyperchromic effects (52.94%), equilibrium constant (K) values (3.51) and negative values of Gibbs free energy (ΔG°) (-3.06 KJ/mol). Co-pigments that contained more free hydroxyl groups facilitated the co-pigmentation, and methyl contributed more to color enhancement, with respect to the hydrogen group. Ultra Performance Liquid Chromatography-Quadrupole-Time Of Flight-Mass/Mass Spectrometry (UPLC-Q-TOF-MS/MS) results indicated that FA and CA formed different anthocyanin derivatives with mulberry anthocyanin. The Fourier Transform Infrared Spectroscopy (FTIR) and molecular docking confirmed that hydrogen bonding, π-π stacking and hydrophobic interaction were formed between anthocyanins and three prevalent co-pigments (FA, CA and VA). CA and C3G could form four hydrogen bonds and two π-π stackings; this was the most stable system among three phenolic acid-C3G complexes. Due to the functional effect of phenolic acids, the addition of FA and CA not only enhanced the stability and color intensity of mulberry anthocyanins but also the functionality of the processing product.

Thermal stability enhancement of berry anthocyanins by co-pigmentation with extracts from natural sources

Abstract The suitability of mandarin (MP), orange (OP) and pomegranate peel powders (PP) for co-pigmentation of dewberry, viburnum, red hawthorn, black hawthorn, and barberry anthocyanins was investigated. Spectrophotometric measurements indicated co-pigmentation causing both a hyperchromic effect (ΔABSmax = 5–13) and bathochromic shift (Δλ vis-max up to 13 nm). The degradation kinetics of anthocyanins were estimated at temperatures ranging from 70 to 90 °C. First-order reactions with rate constants of 0.45–2.93 min−1 and 0.30–2.00 min−1 were observed for the reference and PP co-pigmented samples, respectively. The t 1/2 values were 3.90–25.7 h for the reference and 5.8–38.5 h for the co-pigmented samples. The activation energy (E a) values were higher in co-pigmented samples (49.16–77.77 kJ/mol) than in reference samples (41.82–68.75 kJ/mol), except for black hawthorn, which had a lower E a value in the co-pigmented sample. The thermodynamic parameters (enthalpy, free energy, and entropy) evaluated indicated a positive effect of co-pigmentation on the thermal treatment of anthocyanins.

Loading ferulic acid into β-cyclodextrin nanosponges; antibacterial activity, controlled release and application in pomegranate juice as a copigment agent

Ferulic acid (FA) is a natural antioxidant with antimicrobial activity and its application is restricted due to poor water solubility. To overcome this limitation, FA was entrapped into cyclodextrin nanosponges (CD-NSs). The antibacterial activity of FA was assessed using the microdilution method which showed a higher antibacterial activity after encapsulation. A controlled release of FA was observed in simulated gastrointestinal environment and food simulants. Furthermore, the application of FA-loaded nanosponges (FA-NS) was investigated in pomegranate juice (PJ). Anthocyanins are the main phenolic compounds of PJ that are sensitive to different environmental conditions. Copigmentation of anthocyanins with other phenolic acids can help to improve their stabilization. The antioxidant activity and phenolic content of all PJ samples (free PJ and PJ containing pure FA and FA-NS) were enhanced during storage while the anthocyanin content of all PJ samples was lessened during the storage. However, the samples containing FA-NS compared to the other samples could better preserve the anthocyanin content. Enhanced solubility of FA after incorporation into CD-NSs can induce higher interaction of FA with other components in PJ and improve their stability to preserve during the storage. These results may pave the way for the application of FA-NS as a copigment agent in juices containing anthocyanins to improve their stability during storage.

Copigmentation of Anthocyanin Extract from Parijoto Fruit (Medinilla speciosa) and Its Stability at Different Temperatures and Heating Durations

Parijoto fruit (Medinilla speciosa) has a red color and contains anthocyanins. Anthocyanins have low stability due to the effect of heating. Copigmentation can be applied to improve the stability of anthocyanins. This research aims to study the effect of the anthocyanin extract from parijoto fruit ratio and copigment on anthocyanin concentration and color stability during heating at different temperatures and durations. Anthocyanin copigmentation using tannic acid in a ratio of 1:20 and 1:40 with control without copigmentation (1:0) showed that increasing the tannic acid copigment would increase anthocyanin concentrations and reduce anthocyanin losses due to increased temperature and heating time. The use of 1:40 tannic acid copigment increased anthocyanin concentration by 38%. In addition, increasing the concentration of tannic acid increased the ability of anthocyanins to maintain color retention during heating and increased temperatures. The use of tannic acid at a ratio of 1:40 only decreased color retention by 16%, compared to control (54%) after heating at 75ºC heating for 120 minutes. Copigmentation with tannic acid up to a ratio of 1:40 was able to maintain the stability of the lightness (L*), the reddish value (a*), and the yellowish value (b*) of the anthocyanins. This study showed that this system is potential for food coloring application in the food industry.

Co-pigmentation of strawberry anthocyanins with phenolic compounds from rooibos

Anthocyanin-rich strawberry model solutions were co-pigmented with rooibos phenolics to enhance color and heat stability. The addition of green and fermented rooibos extracts at pigment-to-co-pigment molar ratios of 1:10, 1:50, and 1:100 pelargonidin-3-glucoside equivalents: orientin equivalents induced hyper- and bathochromic shifts at room temperature and during thermal processing at 80 °C for an hour. Co-pigmentation effects on hyperchromic shift were up to 96%, and bathochromic shift reached 19 nm when adding flavonoid-rich fractions of green rooibos phenolics. Following the co-pigmentation tests with rooibos extracts, selected pure phenolic co-pigments were tested for their monomeric contribution to the observed co-pigmentation effects. Orientin was identified as a potent co-pigment for pelargonidin-3-glucoside, showing stronger co-pigmentation effects than that of its aglycon luteolin. Additionally, orientin had the most pronounced bathochromic shift in heat-treated solutions. Rooibos extracts, particularly flavonoid-rich fractions composed of luteolin, apigenin, and quercetin glycosides, are suggested as color enhancers and stabilizers for strawberry products.

Addition of Medicinal Plants Increases Antioxidant Activity, Color, and Anthocyanin Stability of Black Chokeberry (Aronia melanocarpa) Functional Beverages.

The present study investigates the effect of the addition of medicinal plants, such as lady’s mantle, lavender, rosehip, and meadowsweet, on the chemical composition, antioxidant activity, and color intensity of ready-to-drink aronia nectar during pasteurization and long-term storage. Pasteurization caused a significant decrease in anthocyanin content of aronia nectar, which reduced to 20% of the initial value after four months of storage. Herbs provided different protection to aronia anthocyanins that degraded more slowly during the four-month storage compared to pasteurized control without herbs. The addition of medicinal plants enriched aronia nectar with phenolic compounds and increased its antioxidant activity by up to 52% in meadowsweet-aronia nectar. Moreover, it was accompanied by a color intensity magnification due to co-pigmentation of aronia anthocyanins and herbal phenolics. In contrast to anthocyanins, which constantly degraded during the whole period, color intensity began to stabilize after 30 days, demonstrating that co-pigmentation was progressively established during the time and rosehip provided the best stabilization of aronia nectar color. Current research demonstrates for the first time that medicinal plants such as lady’s mantle, rosehip, and especially meadowsweet can be used to increase antioxidant activity, color, and anthocyanin stability of black chokeberry functional beverages.

Copigmentation and stabilization of anthocyanins using organic molecules and encapsulation techniques

Natural colorants/pigments such as anthocyanins, carotenoids, betalains, and chlorophylls have been generally used in the food industry as coloring agents. However, there are challenges related to color fading during food processing, storage, and commercialization due to the low stability of natural colorants compared to synthetic ones. Among them, anthocyanins are potential food colorants due to their color, low toxicity, and biological properties. However, the low chemical and thermal stability of anthocyanins have limited their use on a commercial scale. Copigmentation is the most commonly used technique for anthocyanin stabilization. This technique provides many advantages associated with the stability of anthocyanins. Natural anthocyanin pigments and copigments (e.g. polymers, phenolic compounds, and carbohydrates) form non-covalent complexes, which stabilize and modulate colors in a wide range of plants, fruits, and food products derived from them (including wines, jams, purees, and syrups). Over the past decade, research studies have been conducted to understand the molecular structure of copigment and the mechanism of copigmentation. This review summarizes this phenomenon to provide a comprehensive description of the interactions between anthocyanin and copigment (the dispersion and electrostatic components of π − π stacking, the hydrophobic effect, and hydrogen-bonding) occurring in copigmentation complexes. The trend of integrating copigmentation and encapsulation has been recently started to develop. In this paper, we also attempt to provide a comprehensive view of the effective approaches including spray and freeze-drying, emulsification, gelation, and their combinations for encapsulating copigmented anthocyanins.

Co-pigmentation of Anthocyanin Jamblang Fruit (Syzygium cumini) with Acetic Acid and Alum

This study aimed to determine the effect of temperature on the stability of anthocyanin jamblang after co-pigmentation with acetic acid and alum. The extraction of Jamblang fruit used the maceration method with n-hexane, ethyl acetate, and ethanol-HCl 0.5% solvents. The total anthocyanin obtained was 70.64 mg/L. Anthocyanin of jamblang fruit was co-pigmented with acetic acid and alum in a concentration of 2.5%; 5%; 7.5%; and 10%, then be heated at various temperatures and heating times. At a temperature of 80 ℃ for 150 minutes, every 30 minutes, absorbance was measured. At a temperature of 60 ℃, the heating was carried out for 225 minutes, and every 45 minutes, absorbance was measured. At a temperature of 40 ℃, the heating was carried out for 300 minutes, and every 60 minutes, absorbance was measured. Absorbance measurements were taken using a UV-VIS spectrophotometer. The results showed that co-pigmentation of anthocyanin occurred proved by shifting λ max from 470 nm (unco-pigmented extract) to 500 nm (co-pigmented extract acetic acid) and 540 nm (co-pigmented extract alum). Acetic acid and alum co-pigments maintained the stability of anthocyanin jamblang at a heating temperature of 60 ℃. It can be seen from decreased absorbance, which is not so significant compared to the decrease at temperatures of 40 and 80 ℃, accompanied by anthocyanin's fading color, which is co-pigmented with acetic acid and alum during the heating process.

β-Cyclodextrin-assisted extraction of phenolic compounds from pomegranate (Punica granatum L.) peel: A new strategy for anthocyanin copigmentation

The present study aimed to conduct co-pigmentation of anthocyanin in pomegranate juice with the extracted pomegranate peel extract (PPE) by the β-cyclodextrin (β-CD) assisted method. The formation of the complex between phenolic compounds and β-CD was approved by FT-IR, DSC, and SEM analyses. The results showed that fortification with PPE significantly enhanced the stability of total phenolic, total flavonoid content, and antioxidant activity during the storage time. Furthermore, a kinetic study of anthocyanin degradation indicated that fortification of juices with PPE decelerated the degradation rate while enhancing their half-life. At the end of the 60-day storage period, the sample containing 600 mg/L PPE showed a higher a* color index than the control sample. Thus, it can be concluded that the PPE- β-CD complex could be used as a co-pigmentation agent in anthocyanin-rich juices to stabilize the color and phenolic compounds.