Cophenetic Research Articles

IRAP and REMAP based genetic diversity among varieties of Lallemantia iberica.

This study describes the genetic relationships among 34 varieties of Lallemantia iberica using inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP). Samples were collected from Agriculture Research Center of Urmia city (northwest Iran). Ten IRAP and REMAP primers generated 76 scorable electrophoretic bands with 78.94% polymorphism. The pair-wise Jacquard genetic similarity varied from 0.48 to 0.94 for IRAP and REMAP data combined. Average PIC values for IRAP and REMAP markers were 0.38. The retro-elements marker system produced 76 alleles in range of 100- 3000 bp. The cophenetic correlation coefficient between Jaccard's similarity matrix and the plotted dendrogram was 0.66. A dendrogram constructed based on COMPLETE LINKAGE. Cluster analysis of IRAP and REMAP data using the NTSYSpc 2.02 resulted in five clusters. The present study represents high genetic distance at genotype level suggesting that IRAP and REMAP markers are useful for Lallemantia iberica genetic diversity analysis.

Relationship between storage protein banding pattern and growth type of some bread wheat varieties.

Seed storage proteins are one of the most important food resources that play important role on bread making quality, in this respect distinguishing the genetic diversity was crucial in breeding aims. In order to study genetic diversity in 20 wheat commercial varieties with different growth type, we analysed the banding patterns of gliadin proteins by SDS-PAGE technique. Our results showed 11 different bands that were polymorphic in 20 studding varieties. Cluster analysis classified these genotypes into 3 groups. These differentiated groups had different growth types; also cluster analysis based on Jaccard similarity coefficient showed that some lines had higher similarities than the others. Principle coordinate analysis showed that 80% of total variance was determined by the first 3 components and cophenetic correlation coefficient equal to 0.75 was resulted and this confirmed the significant relation between gliadins and growth type. Existence of relation between growth type and gliadin proteins could help breeders in variety discrimination and selection perspectives.